Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| binding and internalization of rat colonic mucins by the galactose/n-acetyl-d-galactosamine adherence lectin of entamoeba histolytica. | purified rat colonic mucins inhibit entamoeba histolytica in vitro adherence to and lysis of colonic epithelial cells by binding to the amoebic galactose/n-acetyl-d-galactosamine (gal/galnac)-inhibitable adherence lectin. we found that 125i-labeled mucins demonstrated saturable gal-specific binding to e. histolytica trophozoites (strain hm1:imss), with 2.8 x 10(3) binding sites per amoeba and a dissociation constant of 8.20 x 10(-11) m-1. inhibition of parasite protein synthesis completely abrog ... | 1988 | 2900266 |
| efficacy of a single dose of secnidazole in the treatment of acute and chronic amoebiasis. | an open study was carried out to determine the clinical efficacy of secnidazole in the treatment of acute and chronic cases of amoebiasis. patients were given 2 g of secnidazole in a single dose or in two divided doses within a 4 h interval. details were recorded daily by patients of complaints such as fever, lumbar pain, lbm, dizziness, body weakness, bloody mucoid stools, epigastric pains and other symptoms, before and after treatment. results were analysed for 45 chronic and five acute cases ... | 1988 | 2900339 |
| observations on the interpretation of amoebic serology in endemic areas. | antibody to entamoeba histolytica antigen was evaluated in cases of proven and suspected intestinal amoebiasis as well as in extra-intestinal cases from an endemic area. screening methods included the gel diffusion precipitation test (gd), counter immunoelectrophoresis (cie) and the indirect haemagglutination test (iha). control populations consisting of asymptomatic cyst passers, non-amoebic individuals, patients with giardiasis and cases of enteric fever were also screened using the above test ... | 1988 | 2900340 |
| plant based antiamoebic drugs; part ii. amoebicidal activity of parthenin isolated from parthenium hysterophorus. | 1988 | 2900531 | |
| [analysis of parasitic zymodemes]. | 1988 | 2907394 | |
| effect of histamine and serotonin on phagocytic activity of entamoeba histolytica. | 1988 | 2907510 | |
| application of a micro enzyme-linked immunosorbent assay (elisa) to detection of anti-amebic antibody in various forms of amebic infection. | techniques of a micro enzyme-linked immunosorbent assay (elisa) used for the serodiagnosis of schistosomiasis were applied to amebic infection. test sera were divided primary on the basis of serologic diagnosis and stool examination as follows; (i) gel diffusion precipitin test (gdp) positive and stool examination positive: 9 specimens, (ii) gdp positive and stool examination negative: 29 specimens, (iii) gdp negative and stool examination positive: 32 specimens. virtually all of the individuals ... | 1988 | 2907591 |
| activation of macrophages by entamoeba histolytica extracts in mice. | the effect of entamoeba histolytica extracts on the production of inflammatory macrophages and the release of hydrogen peroxide (h2o2) and superoxide (o2-) from these cells was examined in c57bl/6 mice. two different strains of e. histolytica, either virulent (ip:0682:1) or nonvirulent (dkb), were used in this study. the number of macrophages recovered from the peritoneal cavity of mice treated 5 days previously with 150 micrograms of either strain of amoebic extracts was significantly higher th ... | 1988 | 2907598 |
| action of cholesterol on virulence related biochemical functions of entamoeba histolytica. | 1988 | 2907902 | |
| long shelf-life of antigen-sensitised erythrocytes by double aldehyde stabilisation for the serodiagnosis of amoebiasis by indirect haemagglutination. | 1988 | 2908043 | |
| use of microdilution to assess in vitro antiamoebic activities of brucea javanica fruits, simarouba amara stem, and a number of quassinoids. | a microdilution technique for the assessment of in vitro activity against entamoeba histolytica was devised and validated with metronidazole. the test was used to detect the antiamoebic activities of plant extracts prepared from the traditional remedies brucea javanica fruits and simarouba amara stems. the activity was associated with quassinoid-containing fractions. the 50% inhibitory concentrations for some quassinoids against amoebae were determined by using the microdilution method. these co ... | 1988 | 2908094 |
| [studies on surface electricity of entamoeba histolytica]. | 1988 | 2908101 | |
| biological evidence of genetic exchange in entamoeba histolytica. | the demonstration of a new zymodeme of entamoeba histolytica produced in culture from cloned isolates suggested possible genetic exchange in this parasite. we have attempted to substantiate that finding by using rats as biological hosts. clones were made from 3 separate isolates of e. histolytica, each established in culture from liver pus or faeces. after enzyme characterization these clones, of zymodemes ii, xiv and xix, were paired in each of the 3 possible combinations and the mixtures injec ... | 1988 | 2908287 |
| intestinal lumen and mucosal microclimate h+ and nh3 concentrations as factors in the etiology of experimental amebiasis. | axenically cultivated entamoeba histolytica, trophozoites, strains hm-1:imss and hm-38, were suspended in solutions of nacl, 330 mosm, of varying ph and ammonium concentrations. short-term viability was inversely proportional to the ammonia concentration of the medium and was independent of the ammonium concentration and ph. the nh3-induced ameba killing was associated with a cellular alkalosis and cell swelling. while short-term trophozoite viability was unaffected by changes in the medium ph o ... | 1988 | 2908580 |
| [the incidence of amebiasis in abortion]. | 1988 | 2908694 | |
| movement of entamoeba histolytica trophozoites in rat cecum and colon intact mucus blankets and harvested mucus gels. | entamoeba histolytica trophozoites were centrifuged into mucus gels harvested from in vivo loops of rat cecum and proximal colon. frank ameba movement was not detected in the colonic mucus, but attenuated motility was measured in the cecal mucus. the harvested rat cecal mucus had significantly lower apparent viscosity and neutral glycoprotein concentration values than the colonic mucus. a shape factor method was developed to assess the motility of amebae in mucus gels and intact mucus blankets. ... | 1988 | 2902807 |
| on the specificity of a cysteine proteinase from entamoeba histolytica. | a cysteine proteinase has been isolated from the virulent strain hm1:imss of entamoeba histolytica by two gel chromatography steps, ion exchange chromatography on deae-cellulose and affinity chromatography on organomercurial-sepharose. the purified proteinase was homogeneous, as judged by polyacrylamide gel electrophoresis in the presence and in the absence of sodium dodecyl sulphate, and was a monomeric protein with a relative molecular mass of mr 27000 +/- 2000 possessing an n-terminal alanine ... | 1988 | 2902852 |
| outcome of untreated infection with entamoeba histolytica in homosexual men with and without hiv antibody. | among homosexual men the prevalence of infection with entamoeba histolytica is high. to determine the clinical importance of this infection 55 homosexual men carrying the parasite were investigated in detail. no clinical, serological, or histological evidence of invasive amoebiasis was found in any of them. the patients were not treated and were followed up for 12 to 29 months (mean 21.6 months), during which period none developed symptoms that could be attributed to e histolytica. spontaneous l ... | 1988 | 2902889 |
| enzyme-linked immunosorbent assay in the detection of antigen in amoebic liver abscess. | 1988 | 2903119 | |
| in vitro and in vivo studies of macrophage functions in amebiasis. | experimental intrahepatic inoculation of the gerbil with entamoeba histolytica trophozoites was used as a model of liver amebiasis to study the cellular immune response elicited by the parasite. it was shown that abscess-derived macrophages (5 to 20 days old) were deficient in their capacity to develop a respiratory burst, to secrete and express membrane-bound interleukin-1-like activity, and to kill e. histolytica trophozoites as well as to respond to lymphokines in vitro. however, macrophages ... | 1988 | 2903124 |
| amoebic liver abscess in a bisexual man. | a bisexual man who had not travelled outside the u.k. had an amoebic liver abscess. although it is reported that entamoeba histolytica is usually of a non-pathogenic type in homosexual men in the u.k., it is important to diagnose invasive amoebiasis in this high-risk group so that appropriate treatment can be given. | 1988 | 2903199 |
| nucleotide sequence analysis of an entamoeba histolytica ferredoxin gene. | a cdna clone (subclone b) previously isolated from the human parasite entamoeba histolytica was characterized. dna sequence analysis of subclone b identified the dna as that encoding apoferredoxin. e. histolytica ferredoxin cdna contains unusually short 5' and 3' noncoding regions of 9 and 25 nucleotides, respectively. a genomic ferredoxin clone was isolated from e. histolytica dna, and comparison of genomic and cdna sequences revealed that the ferredoxin gene is unspliced. the deduced amino aci ... | 1988 | 2903444 |
| pehps medium: an alternative for axenic cultivation of entamoeba histolytica and e. invadens. | knowledge of entamoeba histolytica biology in the last 17 years has been acquired largely as a consequence of this parasite's axenic cultivation in tps-1 or tyi-s-33 media. unfortunately, there are often low yields in these media, due to variability of their main components, panmede and yeast extract. we describe a medium, pehps, of which the main components are extracts of ox liver, and ox and swine pancreas (ehp). 5 strains of e. histolytica and 2 of e. invadens were quickly and easily adapted ... | 1988 | 2903589 |
| invasive amebiasis in naturally infected new world and old world monkeys with and without clinical disease. | histopathological preparations of cecum and colon from monkeys naturally infected with invasive entamoeba histolytica were examined to determine the distribution of amebae in the tissues and the types of lesions, if any, associated with them. infections were studied in 3 new world species (10 callicebus moloch, 1 c. torquatus, and 2 aotus trivirgatus) and 3 old world species (8 macaca mulatta, 6 erythrocebus patas, and 1 cercopithecus aethiops). amebiasis was recorded as the principal or a contr ... | 1988 | 2903689 |
| entamoeba histolytica: role of amebic proteinases and polymorphonuclear leukocytes in acute experimental amebiasis in the rat. | the injection of 1 x 10(6) trophozoites of axenically grown entamoeba histolytica strain hm-1 in the subcutaneous tissue of the rat results in an acute and self-limited inflammatory process, characterized by the early onset of conspicuous tissue necrosis and focal hemorrhage in the vicinity of the parasites, followed by infiltration with polymorphonuclear leukocytes. the process develops for 5-10 hr but during that period amebic trophozoites progressively disappear, leukocytes undergo degenerati ... | 1988 | 2903831 |
| the prevalence of entamoeba histolytica in lactating women and in their infants in bangladesh. | entamoeba histolytica was studied in 33 lactating women and their infants in a periurban village in bangladesh. infant-mother pairs were followed for a period of 10-15 months: 67% of mothers excreted e. histolytica during the observation period, the majority for 3 months or more. only one mother was symptomatic, with a mild, non-dysenteric diarrhoea. 58% of mothers were seropositive, several of them continuously and with a high titre, indicating past invasive infection. 67% had detectable antibo ... | 1988 | 2902705 |
| immunological differentiation of pathogenic and non-pathogenic isolates of entamoeba histolytica. | entamoeba histolytica can act as a harmless commensal organism in the lumen of the large intestine, or can cause invasive amoebiasis. some workers have suggested that there are two distinct subspecies of this organism, and that only one of these is associated with invasive disease. present isoenzyme tests to identify the subspecies take several days to analyse: we report a technique that uses immunofluorescence with monoclonal antibodies, takes two days to perform, and may, therefore, assist in ... | 1988 | 2894495 |
| parasitic pneumonia. | with few exceptions, the parasitic pneumonias most commonly encountered in the western hemisphere are diseases of compromised hosts; patients with aids are at particular risk. pneumocystis carinii pneumonia occurs eventually in 80% of aids patients; bronchoalveolar lavage is quite sensitive in establishing this diagnosis. toxoplasma gondii pneumonia, seen most often in the patient with aids, is characterized by multisystem involvement. strongyloides stercoralis infection is endemic in the southe ... | 1988 | 3041517 |
| aetiologic agents of diarrhoeal diseases in hospitalised children in rawalpindi, pakistan. | the causes of diarrhoea were diagnosed in 250 children and 250 controls, a sample of 3,500 children who reported to the rawalpindi general hospital, pakistan between may 1983 and april 1984. the pathogens identified in children with diarrhoea were enteropathogenic escherichia coli (epec) (43.2%), enterotoxigenic e. coli (14%), rotavirus (9.6%), shigella spp (3.6%), salmonella spp (3.2%) and giardia lamblia (1.2%). mixed infections were found in 2.4% of patients. the following pathogens were dete ... | 1988 | 3077947 |
| evaluation of an enzyme-linked immunosorbent assay in the serodiagnosis of amoebic liver abscess. | an indirect enzyme-linked immunosorbent assay (elisa) was evaluated for the detection of anti-amoebic igg and igm antibodies to assess its value in distinguishing past from current infection in invasive amoebiasis, particularly in amoebic liver abscess (ala) patients. using sera from 295 individuals, the elisa was also compared with the amoebic gel diffusion (agd) test. in 100 patients the igg-elisa at a single test dilution of 1/6,400 had a sensitivity of 99% for clinically diagnosed ala. in th ... | 1988 | 3144760 |
| [acute idiopathic ulcerative rectocolitis in asymptomatic intestinal amebiasis caused by entamoeba histolytica. diagnostic and therapeutic problems]. | 1988 | 3175271 | |
| a survey of gastrointestinal parasites in ogun state, southwest nigeria. | to study the epidemiology of intestinal parasites in ogun state, nigeria, 479 stool specimens were examined at a hospital in abeokuta during the rainy season in june 1986. intestinal helminths and/or protozoa were present in 297 samples (62%). 41.1% of the specimens showed a single infection, 34% showed double, 20% triple and 5% quadruple infection. the most commonly found worm was ascaris lumbricoides (in 40% of specimens), followed by trichuris trichiura (23.2%), hookworms (19.2%), strongyloid ... | 1988 | 3178337 |
| enzyme immunoassay for detection of giardia lamblia cyst antigens in formalin-fixed and unfixed human stool. | an antigen-capture enzyme-linked immunosorbent assay employing rabbit and mouse antisera to giardia lamblia cyst antigens was developed for the diagnosis of giardia infection through detection of g. lamblia-specific stool antigens in cell-free aqueous eluates of human stool. this is the first report of the use of anti-cyst antibodies in an enzyme immunoassay for g. lamblia. the assay gave a positive result with 54 of 59 stools from patients with symptomatic, clinically diagnosed giardiasis, givi ... | 1988 | 3183015 |
| the microbial causes of diarrhoea in patients infected with the human immunodeficiency virus. | diarrhoea is common in patients infected with the human immunodeficiency virus (hiv). we sought the cause of diarrhoea in all hiv antibody-positive patients with diarrhoea who presented at st stephen's hospital, london over a period of 15 months. altogether, 441 specimens from 179 patients were examined. infective causes were found in 86 (48%) patients. protozoa were the most common infecting organisms (30% patients). of these, cryptosporidium sp. was the most frequent (9.5% patients), followed ... | 1988 | 3183401 |
| zoonotic parasite infections of the arabian sacred baboon papio hamadryas arabicus thomas in asir province, saudi arabia. | evidence of zoonotic parasite infection was recorded in the faeces of the arabian sacred baboon in human populated residential and non-residential areas in asir, southwestern saudi arabia. cysts of giardia intestinalis and entamoeba histolytica, and ova of hymenolepis nana, enterobius vermicularis, ascaris sp., trichuris sp., and a hookworm were encountered in 45 (about 39%) of 115 faecal samples examined. a higher rate of infection was observed in the residential areas. most of the parasites re ... | 1988 | 3232917 |
| intestinal parasites in children with diarrhea in el salvador. | in a study of parasitological prevalence in el salvador, stool specimens of 210 children with diarrhea were examined for intestinal parasites. in 104 cases (49%), intestinal helminths and protozoa were found. 53.4% of the specimens showed single infestation, 31.7% showed double. 3% triple and 1% quadruple infestation. of the helminths, 31% were trichuris trichiura, 18% ascaris lumbricoides and 5% hymenolepis nana. of the pathogenic protozoa, 7% were identified as giardia intestinalis, 6% entamoe ... | 1988 | 3262555 |
| inferior vena caval and right atrial thrombosis complicating an amebic hepatic abscess. | we describe a 50-yr-old black laborer who presented with right lower chest pain, weight loss, and pedal edema. ultrasonography and computed tomograms showed a large abscess cavity in the right lobe of the liver which extended very close to the inferior vena cava. the lumen of the adjacent inferior vena cava was partially occluded by thrombus, which could be traced up into the cavity of the right atrium. the hepatic veins were normally patent. sterile blood-stained pus was aspirated from the absc ... | 1988 | 3289381 |
| ova, larvae and cysts in fingernail contents. | 1988 | 2892676 | |
| epidemiologic and clinical features of invasive amebiasis in bangladesh: a case-control comparison with other diarrheal diseases and postmortem findings. | to describe the epidemiologic and clinical features associated with invasive amebiasis in bangladesh, 85 hospitalized diarrheal patients with hematophagous trophozoites of entamoeba histolytica in their stools were compared to a control group of 84 hospitalized diarrheal patients without amebiasis. postmortem examinations were carried out in 22 deaths due to amebiasis. for the patients with amebiasis, there was a bimodal age distribution with peaks at 2-3 years and greater than 40 years, whereas ... | 1988 | 2895590 |
| antigens of entamoeba histolytica recognized by immune sera from liver abscess patients. | immune sera from 11 patients cured of amebic liver abscess was used to identify antigens of entamoeba histolytica. strain hm1-imss, among the most virulent in axenic culture, was used. the 37 and 90 kd antigens were surface glycoproteins as indicated by lactoperoxidase iodination and by concanavalin a blotting; the 59 kd antigen was a mannose containing glycoprotein that did not appear to be on the cell surface. western blots of 11 different immune sera revealed specific binding of immune igg to ... | 1988 | 2893553 |
| appearance of sialoglycoproteins in encysting cells of entamoeba histolytica. | amoeba-bacterium cultures of entamoeba histolytica transferred to a hypoosmotic medium depleted of nutrients changed morphologically and biochemically. the cells ejected grains of rice starch, rounded up, and formed a distinct cell wall that was resistant to detergent, bound the sialic acid-specific lectin from limulus polyphemus, and became fluorescent with calcofluor m2r. a subpopulation of these cells displayed more than one nucleus. all these signs are characteristic of encysting cells and w ... | 1988 | 2893775 |
| amebic liver abscess in a european patient: zymodeme classification of entamoeba histolytica. | this is a case report of a 36-year-old patient who developed an amebic liver abscess after a stay in the sudan. he was first misdiagnosed as having pneumonia of the right lower lobe. following establishment of the correct diagnosis, the patient recovered fully after metronidazole treatment. the fecal culture in robinson's medium yielded extensive growth of entamoeba histolytica. electrophoretic characterization proved it to be a zymodeme xix, which is one of the zymodemes associated with pathoge ... | 1988 | 2893858 |
| comparison of repeated dna from strains of entamoeba histolytica and other entamoeba. | restriction enzyme digestion patterns of total genomic dna revealed the presence of highly repeated dna in entamoeba histolytica and e. histolytica-like (laredo type) amoebae of humans, e. invadens and e. invadens and e. terrapinae of reptiles, and e. moshkovskii isolated from sewage polluted waters. homology with plasmodium berghei ribosomal dna was striking. northern blot analysis of e. histolytica rna provided further evidence that the highly repeated dna codes for ribosomal rna. the distribu ... | 1988 | 2893976 |
| phorbol esters specifically enhance the cytolytic activity of entamoeba histolytica. | entamoeba histolytica causes invasive amebiasis by lysis of host tissue and inflammatory cells. the in vitro cytolysis of target chinese hamster ovary (cho) cells by axenic e. histolytica trophozoites (strain hm1:imss) is a calcium- and phospholipase a-dependent event initiated by the binding to the target cell of the galactose-inhibitable surface lectin of the parasite. we utilized phorbol esters as a probe to determine whether an amebic protein kinase c has a role in the cytolytic event. the a ... | 1988 | 2897334 |
| relationship of free intracellular calcium to the cytolytic activity of entamoeba histolytica. | entamoeba histolytica adherence and destruction of host cells is required for in vivo pathogenicity; amebic in vitro adherence is mediated by a galactose- or n-acetyl-d-galactosamine-inhibitable surface lectin (gal/galnac adherence lectin). free intracellular ca2+ concentration [( ca2+]i) was measured in living amebae and target cells during amebic cytolysis of chinese hamster ovary (cho) cells and human polymorphonuclear neutrophils by utilizing the ca2+ probe fura-2 and computer-enhanced digit ... | 1988 | 2897335 |
| interaction between pathogenic amebas and fibronectin: substrate degradation and changes in cytoskeleton organization. | invasion of human tissues by the parasitic protozoan entamoeba histolytica is a multistep process involving, as a first step, the recognition of surface molecules on target tissues by the amebas or trophozoites. this initial contact is followed by the release of proteolytic and other activities that lyse target cells and degrade the extracellular matrix. in other parasitic diseases, as well as in certain cancers, the interaction of invasive organisms or cells with fibronectin (fn) through specif ... | 1988 | 2897372 |
| the immune response in amoebiasis. | 1988 | 2897401 | |
| amebiasis. the ancient scourge is still with us. | amebiasis is usually contracted in geographic areas where sanitation is poor, but outbreaks can still occur anywhere that drinking water becomes contaminated with sewage. in the majority of persons infected with the parasite, colonization of the intestine is asymptomatic. in others, symptoms of gastrointestinal distress can appear within a week. in rare cases, extra-intestinal amebiasis can cause abscesses in the liver or elsewhere. many questions about the disease course in different patients r ... | 1988 | 2897682 |
| [colitis--recent trends and countermeasures. mycoses, protozoan and parasitic diseases]. | 1988 | 2898028 | |
| factors influencing the pathogenicity of entamoeba histolytica. | 1988 | 2898182 | |
| amoebic pericardial effusion. | 1988 | 2898540 | |
| [possible entry of fragments of a bacterial genome into the trophozoite nucleus of the dysentery amoeba]. | the use of fölgen-like electron microscopy reaction has revealed particles in the cytoplasm and nucleus of the dysentery amoeba trophozoites which in their size and resistance to acidic hydrolysis and in their affinity for uranilacetate are similar to tightly packed elements of the bacterial genome. on this basis a conclusion was drawn that some fragments of genomes of phagocytic bacteria can be preserved in the amoebal cell. the community of microorganisms consisting of trophozoites of the dyse ... | 1988 | 2898747 |
| affinity purification and biochemical characterization of histolysin, the major cysteine proteinase of entamoeba histolytica. | we report a one-step method for the purification to homogeneity of a cysteine proteinase of entamoeba histolytica (histolysin) by affinity chromatography of the soluble extract of the parasite on immobilized phenylalanyl(2-phenyl)aminoacetaldehyde semicarbazone. the enzyme has an apparent mr of 26,000 by sds/polyacrylamide-gel electrophoresis and 29,000 by gel chromatography. its ph optimum varies widely, from 5.5 with azocasein to approx. 7 with other protein substrates and benzyloxycarbonylphe ... | 1988 | 2898937 |
| in vitro killing of entamoeba histolytica trophozoites by interferon-gamma-activated mouse macrophages. | the effect of murine interferon gamma (ifn-gamma) on macrophage activation for amoebicidal activity was examined. peritoneal macrophages were harvested from c57bl/6 mice and preincubated with ifn-gamma and/or lipopolysaccharide (lps). in vitro amoebicidal activity of these macrophages was determined by trypan blue exclusion test against a virulent strain of e. histolytica (ip:0682:1). it was found that in vitro amoebicidal activity was evident in macrophage monolayers treated with both ifn-gamma ... | 1988 | 2899056 |
| [the importance of entamoeba histolytica in persons returning from the tropics]. | stools from 1000 travellers returning from the tropics were examined for intestinal parasites. the most frequently isolated intestinal pathogens were giardia lamblia (5%) and e. histolytica (3.6%). for 21 out of 36 e. histolytica isolates iso-enzyme differentiation by starch-gel electrophoresis was possible, yielding a pathogenic pattern in four. this finding correlated closely with clinical symptoms and positive serology, while e. histolytica with non-pathogenic zymodeme should not be considere ... | 1988 | 2896113 |
| amoebic hepatitis as a precursor of suppurative amoebic liver abscess. | 1988 | 2896166 | |
| travelers' diarrhea in west africa and mexico: fecal transport systems and liquid bismuth subsalicylate for self-therapy. | the goals of this study were threefold: to compare the etiology of travelers' diarrhea in west africa and mexico, to evaluate two fecal transport systems for the recovery of enteropathogens, and to verify the efficacy of liquid bismuth subsalicylate (bss) in different locations and under different entrance criteria for disease severity. the study populations consisted of 133 european tourists in west africa and 112 american students in mexico who had suffered from travelers' diarrhea. in 60% and ... | 1988 | 2896219 |
| use of chinese hamster ovary cells with altered glycosylation patterns to define the carbohydrate specificity of entamoeba histolytica adhesion. | we compared the adherence of e. histolytica trophozoites with a panel of lectin-resistant cho mutants with altered glycosylation patterns. our results coupled with data from saccharide inhibition studies indicate that terminal n-acetyllactosamine units on asn-linked complex type oligosaccharides provide the major determinants on the cellular receptor for e. histolytica adhesion. | 1988 | 2896759 |
| pathogenic entamoeba. | 1988 | 2896984 | |
| free fatty acids released from phospholipids are the major heat-stable hemolytic factor of entamoeba histolytica trophozoites. | the major hemolytic activity of entamoeba histolytica trophozoites is located in a vesicular fraction called p30 and known to be due to heat-labile and heat-stable hemolytic components whose effect increases up to 100 times during preincubation at 36 degrees c. the heat-stable hemolytic activity (hsha) was found in the chloroform-methanol extract of preincubated p30, whose partition with 2 m kcl yielded a lipid fraction, an interphase, and an aqueous phase. hsha was detected only in the lipid fr ... | 1988 | 2894362 |
| investigation of human stool samples from the cape verde islands (district santa cruz/santiago). | based on the rate of occurrence of diseases associated with diarrhoea, stool samples were also investigated; parasitologically, using the mifc-enrichment method for intestinal parasites; serologically, using elisa for rotavirus and bacteriologically for shigella and salmonella on the cape verde islands. 90% of the samples came from children aged between 6 and 14 years. entamoeba histolytica, the causal agent of amoebic dysentery was demonstrated in 17.4%, lamblia intestinalis in 28.7% and rotavi ... | 1988 | 2844628 |
| the cellular regulation of vesicle exocytosis by entamoeba histolytica. | we studied the cellular regulation of vesicle exocytosis by entamoeba histolytica utilizing release of endocytosed 125iodine (125i) labeled tyrosine conjugated dextran; 125i-dextran entered the acid ph vesicles of the amebae and was not degraded during these studies. exocytosis was temperature dependent with 74%, 36%, 4%, and 0% of 125i-dextran released after 120 min at 37 degrees c, 31 degrees c, 25 degrees c, and 4 degrees c, respectively (p less than 0.01 for each). exocytosis at 37 degrees c ... | 1988 | 2452880 |
| evaluation of faecal preservation and staining methods in the diagnosis of acute amoebiasis and giardiasis. | the use of a faecal preservative and several staining methods, together with formalin ether concentration, were evaluated for the improved diagnosis of intestinal amoebiasis and giardiasis in 1285 patients with diarrhoea or dysentery and from asymptomatic controls. all samples were screened by three wet mount techniques. thirty eight specimens of diarrhoeal or dysenteric stool were preserved in polyvinyl alcohol (pva) and stained by trichrome and spencer and monroe short iron haematoxylin stain. ... | 1988 | 2454958 |
| secretory hydrolases of entamoeba histolytica. | cells of entamoeba histolytica grown over a period of four days contained nadp+-dependent alcohol dehydrogenase exclusively inside the cells. no activity of this enzyme could be found in the growth medium after harvesting the cells. under the same conditions, acid phosphatase, beta-n-acetylglucosaminidase, esterase, alpha-glucosidase, and different amylases of the parasite were found both inside the cells and in the medium. the activities present in the cell homogenate and in the medium before a ... | 1988 | 2456386 |
| bacterial, parasitic and viral enteropathogens associated with diarrhoea in saudi children. | in a 2-year study of stools from saudi children with gastro-enteritis and from controls, rotavirus was the pathogen most frequently detected, either alone (44.3%) or in combination with other enteropathogens (7%). there were two peaks for rotavirus isolates, one during the cold months and the other during the dry, hot season. salmonella spp. and campylobacter jejuni were the second and third most frequently isolated enteropathogens. enteropathogenic escherichia coli did not contribute significan ... | 1988 | 2456718 |
| pathogenic and non-pathogenic entamoeba: pore formation and hemolytic activity. | pore-forming activity in planar lipid bilayers and liposomes of extracts from differentially pathogenic entamoeba and the capacity of trophozoites and subcellular fractions to lyse human red blood cells (hrbc) were investigated. in all amebas studied, the two activities paralleled each other. they were high in e. histolytica irrespective of the virulence of the particular strain, but low in non-pathogenic e. histolytica-like amebas of human origin as well as in e. invadens, which is pathogenic f ... | 1988 | 2460621 |
| inflammatory reaction in experimental hepatic amebiasis. an ultrastructural study. | one of the hallmarks of tissue necrosis produced by the human protozoan parasite entamoeba histolytica, the causative agent of human amebiasis, appeared to be the lack of inflammatory reaction to the invading trophozoites. recent evidence suggests, however, that inflammatory cells do appear during early stages of amebic destructive lesions and that they contribute to the establishment of foci of tissue necrosis in intestinal and liver lesions. the present analysis of the fine-structural changes ... | 1988 | 3337207 |
| diagnosis of meleney's synergistic gangrene. | a retrospective and comparative study of 127 case reports of meleney's postoperative progressive synergistic gangrene and of 62 examples of postoperative amoebic skin gangrene, showed that these two entities were clinically indistinguishable and that therefore a purely clinical diagnosis of meleney's gangrene could not be made. furthermore, a critical appraisal of the bacteriological data indicated that a certain diagnosis of meleney's gangrene cannot be provided by the clinical bacteriologist. ... | 1988 | 3349337 |
| potential impact of colonic irrigation on the indigenous intestinal microflora. | under normal physiological conditions, the indigenous intestinal microflora is stable. the stability protects the host from colonization of nonindigenous microorganisms, including pathogens. indigenous microorganisms capable of producing toxins are held in check by competing microflora, but any accidentally absorbed toxins are efficiently detoxified by the liver in the absence of hepatic pathology. antibiotics are known to disrupt the normal flora leading to increased susceptibility to infection ... | 1988 | 3351397 |
| etiology of diarrhea among travelers and foreign residents in nepal. | a bacterial pathogen was isolated from 47% of 328 expatriate patients with diarrhea seen at two medical clinics in nepal in 1986. enterotoxigenic escherichia coli (24%), shigella (14%), and campylobacter species (9%) were isolated most frequently. enteroinvasive and adherence factor-positive e coli were isolated from 2% and 1% of patients, respectively. giardia lamblia was detected in 12% of patients, rotavirus in 8%, and cryptosporidium and entamoeba histolytica each in 5%. blastocystis hominis ... | 1988 | 3404637 |
| acute diarrhoea in adults: aetiology, clinical appearance and therapeutic aspects. | a prospective study of acute diarrhoea was performed during 15 months 1981/1982 and included 731 patients and 240 controls. 43% had been infected abroad. a cluster of travellers with bacterial pathogens was diagnosed in july-august. the following pathogens were found: campylobacter (18%), enterotoxigenic e. coli (6%), salmonella spp. (5%), rotavirus (4%), yersinia enterocolitica (3%), giardia lamblia (3%), shigella spp. (2%), clostridium difficile (2%), enteroviruses (2%) and entamoeba histolyti ... | 1988 | 3406670 |
| blastocystis hominis, a long-misunderstood intestinal parasite. | for over 50 years, blastocystis hominis has been held to be a harmless intestinal yeast-probably frequent in stool samples from man and other primates, but usually ignored except as a possible source of confusion with entamoeba histolytica. more recently, its status as a protozoan parasite has been accepted, and it is now increasingly recognized as an agent of intestinal disease - usually self-limiting but occasionally fatal in monkeys. here, charles zierdt reviews the status o f this intriguing ... | 1988 | 15462991 |
| reducing agents and entamoeba histolytica. | entamoeba histolytica remains an important but enigmatic parasite. it displays both non-pathogenic and invasive pathogenic types, which can be distinguished clinically and by isoenzyme markers. yet as debated in parasitology today last year(1), the relationship between these two forms remains unclear. bacterial associates and reducing agents are known to play on important role in the culture of e. histolytica, and possibly in its differentiation and invasive mechanisms. this article briefly revi ... | 1988 | 15463049 |
| entamoeba histolytica: from intestine to liver. | 1988 | 15463107 | |
| immunopathology of entamoeba histolytica infections. | entamoeba histolytica infects almost 10% of the world's population and results in about 100 000 deaths annually(1). relatively little information is available concerning the immune response and the immunopathology elicited by this parasite, probably due in part to the lack of a truly appropriate animal model(2-4). however, there has been some progress - particularly concerning the interaction of this parasite with cells of the immune system(5,6). this review summarizes the salient features of th ... | 1988 | 15463113 |
| status of intestinal parasitic infections in a remote island, yondo, jeonranam-do: province. | this study was performed to observe the present status of intestinal parasitic infections in a remote island, yondo, located in southern part of jeonranam-do(province), korea. in february and may 1988, total 1,011 individual stool samples were collected and examined for intestinal helminths and protozoa using formalin-ether centrifugal sedimentation technique. the results are summarized as follows: of 1,011 ingabitants examined, 398(39.4%) were positive for intestinal parasites. helminth positiv ... | 1988 | 12811041 |
| entamoeba histolytica: evasion and invasion. | 1989 | 15463124 | |
| murine t-cell clones against entamoeba histolytica: in vivo and in vitro characterization. | eleven t-cell clones were raised from the spleens of balb/c mice hyperimmunized against a crude soluble extract of entamoeba histolytica trophozoites. seven clones were of the lyt-1+, and four of the lyt-23+ phenotype. all clones proliferated in the presence of e. histolytica antigens but not to a purified protein derivative; five clones proliferated to a crude extract of the e. histolytica-like laredo amoebae. ten clones secreted t-cell growth factors in response to e. histolytica antigens. two ... | 1989 | 15493266 |
| characterization of a thiol proteinase in giardia lamblia. | sonicated preparations of giardia lamblia hydrolyze a variety of protein substrates including human immunoglobulin. activity is increased by thiol-activating agents and inhibited by thiol proteinase inhibitors. about 55% of activity remains in the soluble fraction after high-speed centrifugation, and pretreatment with a nonionic detergent results in increased soluble activity. this suggests that the enzyme is membrane bound or associated with subcellular particles. activity elutes as a major pea ... | 1989 | 2584755 |
| exotic diarrhoeal problems and poliomyelitis. | exotic gastrointestinal infections continue to increase as world travel expands. many are debilitating and some are life-threatening. a heightened awareness of their significance and symptomatology could help to prevent unnecessary suffering or death. | 1989 | 2594659 |
| prevalence and seasonal fluctuations of intestinal parasitic infections in the nablus area, west bank of jordan. | a total of 22,970 stool specimens collected from patients attending the central medical laboratory in the city of nablus in the period of 1981-1986 were examined for intestinal parasites. of these 7412 (32.3%) were positive. entamoeba histolytica (22.9%), giardia lamblia (7.3%), and ascaris lumbricoides (5.7%) were the most prevalent intestinal parasites found. other intestinal parasites present included hymenolepis nana, trichomonas hominis, trichuris trichiura, taenia saginata, enterobius verm ... | 1989 | 2596904 |
| comparison of four nitroimidazole compounds for treatment of symptomatic amoebiasis in kenya. | four antiamoebic drugs currently used in many kenyan hospitals and health centres were compared for their efficacy on symptomatic luminal amoebiasis in kiambu, kilifi, and machakos hospitals during this study. the drugs were; the brand metronidazole (flagyl, may & baker, kenya ltd.), the generic metronidazole (metrozol, cosmos ltd., nairobi, kenya), the brand tinidazole (fasigyn, pfizer laboratories ltd.) and the generic tinidazole (tynazole laboratory and allied equipments, kenya ltd). clinical ... | 1989 | 2606014 |
| [synthesis of 2-aryl benzothiazoles and research on its antiparasitic activity]. | several 2-aryl benzothiazoles (with phenyl, naphthyl, thienyl and some oxygenated heterocycles) were synthesized. the anti-parasitic properties of these compounds were evaluated, in vitro and in vivo, against one nematode nippostrongylus brasiliensis and, in vitro against two protozoaires entamoeba histolytica and trichomonas vaginalis. only two compounds exhibited, in vitro a low nematicidal activity. | 1989 | 2610470 |
| a model of hepatic amoebiasis in random bred mice. | this study describes a model of hepatic amoebiasis in random bred mice (mf2). mice were infected by introducing liver tissue from hamsters (mesocricetus auratus), containing about 5 x 10(4) trophozoites of entamoeba histolytica, between adjacent liver lobes. direct inoculation of xenic e. histolytica resulted in mortality within 24 h, whereas monoxenic and axenic strains failed to produce any lesions. serial mouse liver passage resulted in increased lesion score, number of metastatic abscesses, ... | 1989 | 2617583 |
| experimental production of new zymodemes of entamoeba histolytica supports the hypothesis of genetic exchange. | by mixing in culture zymodemes of entamoeba histolytica, clones showing a different pattern from the 2 parents have been obtained on 3 occasions. two new zymodemes (xi alpha- and xxi) have thus been produced, which belong to the third generation of a family of e. histolytica zymodemes apparently obtained by genetic exchange. a hypothesis for the molecular basis of those exchanges is discussed. we report also the observation of probable mutation. | 1989 | 2617647 |
| determination of antiamoebic igg, igm, iga and igg subclasses in sera from patients with amoebiasis by enzyme-linked immunosorbent assay. | the hk-9 strain of entamoeba histolytica was cultured axenically. a crude extract of the trophozoites was used as antigen in the enzyme-linked immunosorbent assay (elisa) for the determination of antiamoebic igg, igm, iga and igg subclasses in sera of patients with e. histolytica infection. sera from amoebiasis patients had significantly higher mean elisa values for all classes and subclasses of immunoglobulins examined compared with healthy controls. the elisa for igg, igm and iga was positive ... | 1989 | 2633348 |
| [prevalence of entamoeba histolytica and other intestinal protozoa among the inhabitants of poznan province over a period of 30 years]. | the aim of the study was to present the past and actual prevalence of entamoeba histolytica and other intestinal protozoa in poznań province. although in poland there are only few accurate data concerning the prevalence of intestinal protozoa, the results of some studies were found reliable. in a survey conducted in 1958 in an orphanage in poznań (kasprzak and karlewiczowa) the intestinal protozoa were found with high prevalence (table 1). after six years this prevalence decreased considerably ( ... | 1989 | 2633499 |
| [pathogenic agents in acute non-enterocolic diarrheal syndrome]. | along a one year period 112 infants admitted with non enterocolic acute diarrhea were studied for isolation of potentially ethiologic agents, namely enteropathogenic bacteria (salmonella, shigella, campylobacter, classic enteropathogenic, enteroinvasive and enterotoxigenic escherichia coli), rotavirus (viral rna electrophoresis) and enteroparasites (telemann and pafs). the most frequently identified pathogen was rotavirus (57.8%), followed by thermo labile toxin producing escherichia coli (19.7% ... | 1989 | 2634863 |
| descriptive epidemiology of persistent diarrhoea among young children in rural northern india. | in order to determine the descriptive epidemiology of persistent diarrhoea in rural northern india, a cohort of 963 children aged 0-71 months was followed prospectively for 12 months through weekly household visits. the incidence of persistent diarrhoea was 6.3 per 100 child-years among those aged 0-71 months, and was highest (31 per 100 child-years) among those aged 0-11 months. there were no significant sex-related differences in the incidence of the disease, and the overall seasonal distribut ... | 1989 | 2670297 |
| [production and characterization of monoclonal antibodies to echinococcus multilocularis]. | spleen cells from balb/c mice immunized against echinococcus multilocularis were fused with mouse myeloma cells (p3x6.5.3.) in the presence of polyethylene glycol (peg 1000), and the hybridomas were obtained. of the 85 hybridomas, 23 were found to produce antibodies. of the 23 cell lines, 13 hybridomas which produced a high titer of antibodies were cultured for cloning the cells by the limiting dilution method. then 5 monoclonal antibodies were obtained. the immunoglobulin class of the monoclona ... | 1989 | 2684834 |
| an outbreak of amebiasis in an institution for the mentally retarded in japan. | the results of an epidemiological survey in a 190-patient institution for mentally retarded were reported. twenty percent of the patients had either cysts or trophozoites of entamoeba histolytica in their stools, and 38% were positive serologically. the amebic outbreak revealed a sex-independent but age-dependent distribution; younger patients had more serious symptoms in cases invasive amebiasis. a high prevalence of amebic infection was found in the heavily retarded patients, and the positive ... | 1989 | 2698965 |
| ige level in parasitic diseases before and after specific therapy. | fifty patients (3-12 years old) with parasitic infestation vz: e. histolytica, oxyuris, h. nana, ascaris and mixed infection were compared to ten healthy age and sex matched normal school children. they were subjected to stool and urine analysis total and differential white count, serum protein and serum ige estimation before and after the specific treatment. the absolute eosinophilic count was high during infestation in all groups and significantly decreased after treatment (p less than 0.01). ... | 1989 | 2708864 |
| [invasive amebiasis as a public health problem]. | amebiasis, an infection of humans with the protozoan entamoeba histolytica, has a wide distribution in mexico. the lumenal, asymptomatic infection, as measured by the presence of cysts in stools has been recorded from 2.4% at ometepec, guerrero to over 55% at mixquic, d.f., but only a small percentage of those having intestinal infection will develop invasive amebiasis, the main clinical forms being dysentery and liver abscess. in mexico city, from 0.8 up to 14% of cases of acute diarrhoea in ch ... | 1989 | 2713060 |
| intestinal infections in patients with acquired immunodeficiency syndrome. a prospective study in 132 patients. | we studied prospectively 132 patients with acquired immunodeficiency syndrome to define the spectrum of enteric pathogens during this disease, with special reference to the correlation between the lesions, the infections, and the symptoms. forty-four percent of the patients harbored at least one enteric pathogen: the most frequently recovered were cryptosporidium (28), cytomegalovirus (16), entamoeba histolytica (13), giardia lamblia (9), and mycobacterium avium intracellulare (7). patients harb ... | 1989 | 2714152 |
| [blastocystis hominis, a parasitic cause of diarrhea]. | the frequency of detection of blastocystis hominis in the stools of patients with gastroenteritis is reported. over a twelve-month period b. hominis was identified in the stools of 69 (4.72%) out of 1460 patients. of these 69 positive samples, 45 (65%) contained b. hominis alone and in 24 (35%) it was present together with other parasites such as lamblia intestinalis, entamoeba histolytica, entamoeba coli, endolimax nana, iodamoeba buetschlii, ancylostoma duodenale, ascaris lumbricoides, trichur ... | 1989 | 2717900 |
| the vitamin b1 status among young egyptian males in relation to infection with parasites. | erythrocytic transketolase activity and thiamin pyrophosphate effect (tpp%) were used to define the nutritional thiamin status of an age stratified sample of 66 young males with parasitic infection. the results revealed that 6% of the control group were biochemically deficient in vitamin b1 compared with a percentage incidence of 42% among the parasitized subjects. intestinal infection with entamoeba histolytica, hymenolepsis nana or with more than one parasite had the most depleting effect on t ... | 1989 | 2722425 |
| entamoeba histolytica: diffuse liver inflammation in gerbils (meriones unguiculatus) with experimentally induced amebic liver abscess. | the histopathological changes that occur in the uninfected liver lobes of gerbils with a localized amebic liver abscess (left lobe) were studied from 5 to 60 days after inoculation. early parenchymal changes (10 days) in the right and median liver lobes showed a lymphocytic infiltrate and bile duct proliferation in the portal areas. maximum alterations occurred at 20 to 30 days after inoculation and were characterized by a dense portal infiltrate, hepatocyte degeneration, granulocytic infiltrate ... | 1989 | 2724182 |
| squamous cell carcinoma associated with amoebic cervicitis. report of a case. | a case of squamous cell carcinoma associated with amebiasis is presented. protozoa identified as entamoeba histolytica were found in a routine papanicolaou smear and in the cervical biopsy of a young woman following the initial diagnosis of cervical cancer and the institution of a radiation therapy regimen. no amoebae were found in repeated stool specimens. the late detection and diagnosis of amebiasis precluded any possible changes in the management of the case. initial follow-up for the carcin ... | 1989 | 2728784 |
| [severe forms of hemorrhagic rectocolitis. predisposing role of the amebic graft?]. | severe forms of ulcerative colitis are somewhat rare but have a severe prognosis. apart from the usual predisposing factors, the amebic graft must be especially emphasized, at least in those countries where the parasite is endemic. entamoeba histolytica was consistently identified in 19 cases of the severe form that we found out of a total of 126 cases of ulcerative colitis. usually the parasite was found in the shape of massive infestation and in most cases it proved to be resistant to the ameb ... | 1989 | 2764495 |
| pattern of intestinal parasitic infection in preschool children in riyadh, saudi arabia. | a total of 1,167 stool specimens collected from 0.6-6 years old patients attending king abdel aziz university hospital (kauh) in riyadh, were examined for intestinal parasites. of these 243 (20.8%) were positive. giardia lamblia (13.5) and enterobius vermicularis (4.2%), were the commonest parasites found. other parasites present include ascaris lumbricoides, entamoeba histolytica and hymenolepis nana. abdominal pain (38.6%) and diarrhoea (27.6%) were the most common causes of referral presented ... | 1989 | 2809181 |