Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| chlamydia trachomatis: probable cause of prostatitis. | seventy-eight men with symptoms of chronic or subacute prostatitis were enrolled. investigations for the presence of chlamydia trachomatis in urethral swabs were carried out. the expressed prostatic secretions were additionally examined for mycoplasma hominis, ureaplasma urealyticum, gardnerella vaginalis, other gram-negative and gram-positive bacteria, trichomonas vaginalis, yeast-like fungi and leucocyte count. furthermore, all patients were evaluated for the presence of serum anti-chlamydial ... | 1998 | 9671250 |
| audit of diagnostic criteria for trichomonas vaginalis in a genitourinary medicine clinic. | a comparison between high vaginal swab (hvs) wet prep examination within the genitourinary medicine (gum) clinic and that made from an hvs transported in amies charcoal medium to the laboratory for the diagnosis of trichomonas vaginalis was made in a prospective study. clinic wet prep compared with amies had a sensitivity of 68% and specificity of nearly 100%. using the clinic wet prep alone, 9/30 (30%) cases would have been missed. | 1998 | 9671254 |
| purification and partial characterization of glyceraldehyde-phosphate dehydrogenase from electric organ of electrophorus electricus (l.). | the glyceraldehyde-phosphate dehydrogenase (gapdh, ec 1.2.1.12) was purified to homogeneity from electric organ of electrophorus electricus (l.) by a hydrophobic chromatography method on deacetylcolchicine-sepharose. the purification resulted in a 162 fold increase in specific activity of the gapdh and final yield was approximately 37%. the purified enzyme showed a single band in sds-page, with an apparent molecular mass of 36 kda. the purity of the colchicine-sepharose isolated material was ana ... | 1998 | 9679332 |
| cytologic identification of trichomonas vaginalis in urine from a male with long-standing sterile pyuria. | 1998 | 9684611 | |
| trichomonas vaginalis thymidine kinase: purification, characterization and search for inhibitors. | we report that a thymidine kinase (tk) activity is present in trichomonas vaginalis and can be separated from the deoxyribonucleoside phosphotransferase. t. vaginalis tk, purified 11200-fold to apparent homogeneity, has a molecular mass of 31500 da. it phosphorylates not only thymidine (km 0.18 microm) but also deoxycytidine (km 0.88 microm) and deoxyuridine (km 0.14 microm). in contrast with t. vaginalis deoxyribonucleoside phosphotransferase, the tk activity is strongly inhibited by novel deox ... | 1998 | 9693096 |
| correlation between aerobic and anaerobic resistance to metronidazole in trichomonads: application of a new computer program for permutation tests. | an indispensable step of any comparative study is testing the concordance between the distribution of phenetic traits and the evolutionary history of the taxa under study. here we present a computer program treept which can perform these tasks on the basis of a permutation test. the use of the program was demonstrated on trichomonad drug sensitivity data. the program can also perform permutation tests analogous to the t-test, analysis of variance (anova) and correlation analysis and is available ... | 1998 | 9694378 |
| phylogenetic relationships of the glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase, from parabasalid flagellates. | over 90% of the open reading frame of gap genes for glycolytic glyceraldehyde-3-phosphate dehydrogenase (gapdh; ec 1.2.1.12) was obtained with pcr from five species of parabasala. with gap1 from trichomonas vaginalis obtained earlier, the data include two sequences each for three species. all sequences were colinear with t. vaginalis gap1 and shared with it as a synapomorphy a 10- to 11-residue insertion not found in any other gap and an s-loop with characteristic features of eubacterial gapdh. ... | 1998 | 9694668 |
| analysis of the uptake of the fluorescent marker 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (bcecf) by hydrogenosomes in trichomonas vaginalis. | the fluorescent dye 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (bcecf) has been widely used as an indicator of cytosolic ph. here we report that bcecf localizes to hydrogenosomes (hydrogen-generating organelles found in several phylogenetically separate groups of anaerobic protists) in trichomonas vaginalis, where it was observable by fluorescence microscopy. its cellular location was confirmed by treatment of bcecf-loaded cells with diaminobenzidine and hydrogen peroxide together wi ... | 1998 | 9696354 |
| prevalence and effects of trichomoniasis in pregnancy. | 1998 | 9707712 | |
| testing promoter activity in the trypanosome genome: isolation of a metacyclic-type vsg promoter, and unexpected insights into rna polymerase ii transcription. | in trypanosomes, most genes are arranged in polycistronic transcription units. individual mrnas are generated by 5'-trans splicing and 3' polyadenylation. remarkably, no regulation of rna polymerase ii transcription has been detected although many rnas are differentially expressed during kinetoplastid life cycles. demonstration of specific class ii promoters is complicated by the difficulty in distinguishing between genuine promoter activity and stimulation of trans splicing. using vectors that ... | 1998 | 9709032 |
| sexually transmitted infections among sex workers in kwazulu-natal, south africa. | the purpose of this study was to investigate the prevalence of sexually transmitted infections, including human immunodeficiency virus (hiv), among female sex workers operating at truckstops in the kwazulu-natal midlands of south africa. | 1998 | 9713913 |
| a clinicobacteriological study on leucorrhoea. | the pathogens like trichomonas vaginalis (4.5%), n gonorrhoeae (2.7%) and c albicans (6.7%) were exclusively present in leucorrhoea. the other potential agents with their respective percentages in normal women and cases of leucorrhoea were u urealyticum (21.2% and 50.2%), actinomyces (29.7% and 41.6%), chlamydia trachomatis (17% and 48.8%), candida-like organisms (clo) (1.2% and 9.5%) and non-group b streptococci (4.2% and 16.7%). the percentages of urethral syndrome (65.8%), vaginal irritation ... | 1998 | 9715025 |
| cytokine and antibody responses in women infected with neisseria gonorrhoeae: effects of concomitant infections. | the levels of interleukin (il)-1, il-6, il-8, il-10, and transforming growth factor-beta in sera and genital tract secretions from women with gonococcal cervicitis and other genital infections were examined. cytokines were not elevated in genital secretions from gonococcus-infected compared with uninfected patients. the level of serum il-6 was higher in gonococcus-infected than in uninfected patients at recruitment. serum, but not local, il-1 and il-6 levels were elevated in patients concomitant ... | 1998 | 9728543 |
| cysteine proteases of trichomonas vaginalis degrade secretory leukocyte protease inhibitor. | sexually transmitted diseases, including trichomoniasis, are risk factors for acquisition of human immunodeficiency virus (hiv) infection. enhancement mechanisms are unknown. secretory leukocyte protease inhibitor (slpi) from saliva appears to prevent transmission of hiv through inhibition of virus entry into monocytic cells in vitro. this study was undertaken to determine if secreted cysteine proteases of trichomonas vaginalis degrade slpi and render it nonfunctional. it was determined if slpi ... | 1998 | 9728551 |
| evidence of independent gene duplications during the evolution of archaeal and eukaryotic family b dna polymerases. | eukaryotes and archaea both possess multiple genes coding for family b dna polymerases. in animals and fungi, three family b dna polymerases, alpha, delta, and epsilon, are responsible for replication of nuclear dna. we used a pcr-based approach to amplify and sequence phylogenetically conserved regions of these three dna polymerases from giardia intestinalis and trichomonas vaginalis, representatives of early-diverging eukaryotic lineages. phylogenetic analysis of eukaryotic and archaeal paralo ... | 1998 | 9729885 |
| cloning and molecular characterization of a cdna clone coding for trichomonas vaginalis alpha-actinin and intracellular localization of the protein. | we have identified and sequenced a cdna clone coding for trichomonas vaginalis alpha-actinin. analysis of the obtained sequence revealed that the 2,857-nucleotide-long cdna contained an open reading frame encoding 849 amino acids which showed consistent homology with alpha-actinins of different species. such homology was particularly significant in regions which have been reported to represent the actin-binding and ca2+-binding domains in other alpha-actinins. the deduced protein was also charac ... | 1998 | 9746598 |
| mycoplasma hominis parasitism of trichomonas vaginalis. | 1998 | 9788469 | |
| high expression, purification, and properties of recombinant homocysteine alpha, gamma-lyase. | homocysteine alpha,gamma-lyase from the anaerobic protozoan parasite trichomonas vaginalis has been cloned from genomic dna using pcr methods and expressed in escherichia coli with a vector containing the t7 promoter. the recombinant homocysteine alpha,gamma-lyase (rhcyase) is expressed as the major protein in the host e. coli cells. the enzyme was purified to approximately 90% purity using heat treatment at 50 degreesc, precipitation steps with polyethyleneimine, polyethylene glycol 8000, and h ... | 1998 | 9790890 |
| isolation and characterization of flagella from trichomonas vaginalis. | as a first step in the biochemical and biomedical analyses of flagella from trichomonas vaginalis the flagella were isolated, purified, and analyzed. the flagella were detached by mechanical shearing and a crude flagellar preparation was obtained by low-speed differential centrifugation. the crude flagellar preparation was subjected to further purification by discontinuous sucrose density-gradient centrifugation. electron micrographs (em) of the purified flagella showed the typical 9 + 2 axonema ... | 1998 | 9797064 |
| heat shock protein 70 family: multiple sequence comparisons, function, and evolution. | the heat shock protein 70 kda sequences (hsp70) are of great importance as molecular chaperones in protein folding and transport. they are abundant under conditions of cellular stress. they are highly conserved in all domains of life: archaea, eubacteria, eukaryotes, and organelles (mitochondria, chloroplasts). a multiple alignment of a large collection of these sequences was obtained employing our symmetric-iterative iteralign program (brocchieri and karlin 1998). assessments of conservation ar ... | 1998 | 9797407 |
| evidence for an uncommon alpha-actinin protein in trichomonas vaginalis. | as part of our ongoing project of identification of actin-binding proteins implicated in the cell transition (flagellate to amoeboid/adherent) of trichomonas vaginalis, we have characterized an alpha-actinin-related protein in this parasite. the protein (p100) has a molecular mass of 100 kda and an isoelectric point of 5.5. a monoclonal antibody raised against this protein co-localizes with the actin network. p100 gene transcripts are co-expressed with actin throughout the cell cycle. analysis o ... | 1998 | 9803416 |
| screening for sexually transmitted diseases in rural women in papua new guinea: are who therapeutic algorithms appropriate for case detection? | the presence of a large reservoir of untreated sexually transmitted diseases (stds) in developing countries has prompted a number of suggestions for improving case detection, including the use of clinical algorithms and risk assessments to identify women likely to be infected when they present to clinics for other reasons. we used data from a community-based study of stds to develop and evaluate algorithms for detection of cervical infection with chlamydia trachomatis or neisseria gonorrhoeae, a ... | 1998 | 9803591 |
| cervical cytology in women with bacterial vaginosis. | 398 symptomatic cases of vaginitis and 43 asymptomatic healthy women were subjected to cervical cytology and microbiological investigations for detection of bacterial vaginosis, g. vaginalis, t. vaginalis, c. albicans and m. hominis. cervical inflammatory changes were reported in 81.4% of cases and 60.4% of controls (p < 0.05) and were significantly associated with bacterial vaginosis and g. vaginalis infection (p < 0.01). cervical inflammation was detected in all cases of trichomonasis. | 1998 | 9805847 |
| the sensitivity of a syndromic management approach in detecting sexually transmitted diseases in patients at a public health clinic in cape town. | to evaluate the sensitivity of a syndromic diagnostic procedure in detecting and treating sexually transmitted diseases (stds) and genital tract infections (gtis). | 1998 | 9807192 |
| case report of balantidium coli in human from south of tehran, iran. | a patient with balantidiasis in an urinary bladder is reported. trichomonas vaginalis were identified and no other cause of the haematuria was apparent. this condition didn't common and may be linked to ectopic balantidium in urinary bladder. | 1998 | 9808912 |
| results of clinically downstaging cervical cancer in a cytological screening programme. | the study was aimed at clinically downstaging cancer of the cervix during a cytological screening programme to find out whether such a strategy may help in yielding a large number of early cases of cancer. a cohort of 6,178 women with different cervical lesions were cytologically examined from april 1971 at queen mary's hospital, lucknow, india. the 8,718 asymptomatic women with healthy cervices were taken as controls. the incidence of cervical dysplasia and malignancy in the study group was fou ... | 1998 | 9812227 |
| chitinolytic activities in trichomonas vaginalis. | chitinolytic activities were identified in the protozoa trichomonas vaginalis. overall chitinase activity assessed using chitine-azure as substrate was 10.93 +/- 1.21 nmoles/min/mg prot. end nonreducing chitobiosidase (exochitinase) and chitotriosidase (endochitinase) activities were shown using p-nitrophenyl-substrates and had specific activities of 4.55 +/- 0.53 and 0.47 +/- 0.06 nmol/min/mg prot, kmapp. = 1.32 mm and 5 microm and ph optimum = 7.0 and 6.1 respectively. beta-n-acetylhexosaminid ... | 1998 | 9754300 |
| [cytoskeletal actin and its associated proteins. some examples in protista]. | many processes, cell motility being an example, require cells to remodel the actin cytoskeleton in response to both intracellular and extracellular signals. reorganization of the actin cytoskeleton involves the rapid disassembly and reassembly of actin filaments, a phenomenon regulated by the action of particular actin-binding proteins. in recent years, an interest in studying actin regulation in unicellular organisms has arisen. parasitic protozoan are among these organisms and studies of the c ... | 1998 | 9754306 |
| evaluation of vaginal infections in adolescent women: can it be done without a speculum? | given that highly sensitive urine-based nucleic acid amplification tests may eliminate the need for speculum exam to diagnose gonorrhea and chlamydia cervicitis, we sought to determine if vaginal infections could be diagnosed without using a speculum. | 1998 | 9755269 |
| genetic variance of trichomonas vaginalis isolates by southern hybridization. | in the present study, genomic dnas were purified from korean isolates (kt8, kt6, kt-kim and kt-lee) and foreign strains (cdc85, ir78 and nyh 286) of trichomonas vaginalis, and hybridized with a probe based on the repetitive sequence cloned from t. vaginalis to observe the genetic differences. by southern hybridization, all isolates of t. vaginalis except the nyh286 strain had 11 bands. therefore all isolates examined were distinguishable into 3 groups according to their banding patterns; i) kt8, ... | 1998 | 9755593 |
| motile organisms in the epididymis? | 1998 | 9756322 | |
| trichomonas vaginalis: expression and characterisation of recombinant s-adenosylhomocysteinase. | the gene encoding s-adenosylhomocysteinase activity (s-adenosylhomocysteine hydrolase, sahh; ec 3.3.1.1) in trichomonas vaginalis has been expressed in escherichia coli to facilitate the characterisation of the enzyme. expression of this gene using the pqe-30 (6xhis n-terminal tag) expression system (qiagen) has enabled the one-step purification of 6 mg of active recombinant enzyme from a 100-ml bacterial culture by affinity chromatography using a nickel-nta matrix. the recombinant enzyme has a ... | 1998 | 9769247 |
| diagnosis of trichomonas vaginalis infection by pcr using vaginal swab samples. | trichomonas vaginalis infection is the most prevalent nonviral sexually transmitted disease (std) in the world. a pcr test using vaginal swab samples for the detection of t. vaginalis was developed to add t. vaginalis infection to the growing list of stds that can be detected by dna amplification techniques. a primer set, btub 9/2, was designed to target a well-conserved region in the beta-tubulin genes of t. vaginalis. all strains (15 of 15) of t. vaginalis tested were successfully detected by ... | 1998 | 9774566 |
| [anti-trichomonas activity of metronidazole in the presence of immunobiological preparations]. | the minimal doses of metronidazole, capable of the irreversible immobilization of trichomonas in the presence of one antiprotozoan preparation and after the addition of recombinant tumor necrosis factor (tnf), recombinant gamma-interferon and complex immunoglobulin preparation (cip) into the culture medium, were determined on 4 t.vaginalis isolates, obtained on day 2 after the inoculation of material from the mucous membrane of the urogenital organs of patients (incubation at 37 degrees c). afte ... | 1998 | 9825505 |
| in vitro anti-trichomonic activity of castela texana. | 1998 | 9836284 | |
| polymerase chain reaction amplification of trichomonas vaginalis dna from papanicolaou-stained smears. | dna from papanicolaou-stained smears was successfully amplified using the polymerase chain reaction (pcr) to investigate if it could be used for retrospective genome studies, such as for the detection of trichomonas vaginalis. dna was isolated from 20 archival papanicolaou-stained smears (19 cervical and 1 seminal fluid samples) and purified by treatment with proteinase k, phenol/chloroform, and ethanol. the method of recovering dna from the papanicolaou-stained smear is very important in the su ... | 1998 | 9839133 |
| characterization of trichomonas vaginalis ap33 adhesin and cell surface interactive domains. | adherence to host target cells is a critical step in establishing infection with the sexually transmitted pathogen trichomonas vaginalis. four parasite surface proteins mediating attachment to vaginal epithelial cells have been identified. one surface protein, termed ap33, was characterized further to identify domains interactive with previously generated antibodies and with host surface sites. n- and c-terminal deletion subclones were generated and tested for reactivity with both mab and rabbit ... | 1998 | 9846736 |
| evolutionary relationship between translation initiation factor eif-2gamma and selenocysteine-specific elongation factor selb: change of function in translation factors. | eubacterial and eukaryotic translation initiation systems have very little in common, and therefore the evolutionary events that gave rise to these two disparate systems are difficult to ascertain. one common feature is the presence of initiation, elongation, and release factors belonging to a large gtpase superfamily. one of these initiation factors, the gamma subunit of initiation factor 2 (eif-2gamma), is found only in eukaryotes and archaebacteria. we have sequenced eif-2gamma gene fragments ... | 1998 | 9847405 |
| the pyrophosphate-dependent phosphofructokinase of the protist, trichomonas vaginalis, and the evolutionary relationships of protist phosphofructokinases. | the pyrophosphate-dependent phosphofructokinase (ppi-pfk) of the amitochondriate protist trichomonas vaginalis has been purified. the enzyme is a homotetramer of about 50 kda subunits and is not subject to allosteric regulation. the protein was fragmented and a number of peptides were sequenced. based on this information a pcr product was obtained from t. vaginalis gdna and used to isolate corresponding cdna and gdna clones. southern analysis indicated the presence of five genes. one open readin ... | 1998 | 9847416 |
| [the treatment of vaginal infections with macmiror and macmiror complex]. | the aim of the present study was to examine the therapeutic possibilities of the wide-spectrum medicament macmiror & macmiror complex for the treatment of the vaginal infections. | 1998 | 9859542 |
| cytodiagnosis of discharge per vaginum. | simple, rapid, inexpensive methods such as ph determination, wet mount, koh mount, amine test, gram staining of vaginal discharge were undertaken prior to pap stained smears of 158 patients of leucorrhoea. we were able to detect non-specific vaginitis (44.30%), trichomoniasis (16.45%), candida vaginitis (9.49%), gonococcal vaginitis (0.63%) and senile vaginitis (12 cases). no specific pathology was shown in 26 cases. 1.69% of the cases were of cervical erosion and 3.79% cases suggestive of squam ... | 1998 | 9866899 |
| biological and biochemical modulation of trichomonas vaginalis kt9 isolate after shifting of culture medium from tps-1 into tym. | to evaluate the biological and biochemical characteristics of trichomonas vaginalis kt9 isolate, the growth and size of trichomonads, pathogenicity in mouse, protein profiles and proteinase activity were examined after shifting the medium from tps-1 into tym. generation time of trichomonads in tym medium was 4.5 hr in comparison to tps-1 with 7.1 hr. size of trichomonads cultured in tps-1 medium (8.5 +/- 0.9 x 6.0 +/- 0.9 microns) was significantly smaller than those in tym medium (10.9 +/- 1.4 ... | 1998 | 9868891 |
| degradations of human immunoglobulins and hemoglobin by a 60 kda cysteine proteinase of trichomonas vaginalis. | the present study was undertaken to investigate the role of cysteine proteinase of trichomonas vaginalis in escaping from host defense mechanism. a cysteine proteinase of t. vaginalis was purified by affinity chromatography and gel filtration. optimum ph for the purified proteinase activity was 6.0. the proteinase was inhibited by cysteine and serine proteinase inhibitors such as e-64, nem, iaa, leupeptin, tpck and tlck, and also by hg2+, but not affected by serine-, metallo-, and aspartic prote ... | 1998 | 9868892 |
| mycoplasma hominis parasitism of trichomonas vaginalis. | 1998 | 9872279 | |
| sexually transmitted organisms in sexually abused children. | to establish the prevalence of sexually transmitted organisms and other genital organisms in potentially sexually abused children. | 1998 | 9875049 |
| growth and survival of trichomonas vaginalis. | this study confirmed that feinberg and whittington medium was suitable for the cultivation and detailed study of the growth cycle of two clinical strains of trichomonas vaginalis under anaerobic conditions. both strains showed a similar growth pattern characterised by early but slow growth, extended duration of the logarithmic phase and limited survival never exceeding 144 h. duration of survival and growth rate were inversely proportional to the inoculum density. growth rate was ph dependent; p ... | 1998 | 9879976 |
| etiology of nongonococcal nonchlamydial urethritis. | the majority of cases of acute nongonococcal urethritis (ngu) are due to causes other than infection with chlamydia trachomatis. pathogens implicated as causes of nonchlamydial nongonococcal urethritis (ncngu) include mycoplasma genitalium, ureaplasma urealyticum, trichomonas vaginalis, and primary infection with herpes simplex virus. in a majority of cases of acute ncngu, no pathogen can be isolated. the etiology of chronic ncngu is unknown. | 1998 | 9891672 |
| urethritis treatment. | this article reviews most common sexually transmitted causes of urethritis in men. outlined are clinical presentation, approach, diagnostic work-up, and treatment for the most common pathogens, as well as the cost issues related to antibiotic therapy. common management problems in urethritis are also discussed. | 1998 | 9891673 |
| [survey of sexually transmitted diseases in the region of rio cuarto]. | sexually transmitted diseases (std) are acquired mainly through sexual intercourse, being one of the most frequent groups of infectious diseases worldwide and consequently an important public health problem. the aim of this paper was to determine the current state of std and to compare different diagnostic methods in the population studied. a total of 1060 samples from vaginal flows, endocervical material and urethral discharge were studied during 3 years. of the total samples, 583 were positive ... | 1998 | 9922478 |
| [the incidence of microbial and fungal species associated with trichomonas vaginalis infection]. | 1998 | 9932007 | |
| trichomonas vaginalis: signs, tests and treatment. | 1998 | 9934153 | |
| the relationship of cocaine use and human immunodeficiency virus serostatus to incident sexually transmitted diseases among women. | to assess the incidence of sexually transmitted diseases (std) in a group of heterosexual women as a function of human immunodeficiency virus (hiv) serostatus and to ascertain the effect of crack cocaine use on these relationships. | 1998 | 9518381 |
| observation of membrane fusion on the interaction of trichomonas vaginalis with human vaginal epithelial cells. | the in vitro cytopathic effect of trichomonas vaginalis on epithelial cells was analyzed through the interaction of two parasite strains with freshly collected human vaginal epithelial cells (hvecs) from normal women. videomicroscopy, light and electron microscopy (scanning and transmission), freeze-fracture, the tracer lanthanum nitrate, and the periodic acid-thio-semicarbazide-silver proteinate techniques were used to analyze regions of close contact between the hvecs and t. vaginalis. after 2 ... | 1998 | 9521011 |
| the primitive protozoon trichomonas vaginalis contains two methionine gamma-lyase genes that encode members of the gamma-family of pyridoxal 5'-phosphate-dependent enzymes. | methionine gamma-lyase, the enzyme that catalyzes the breakdown of methionine by an alpha,gamma-elimination reaction and is a member of the gamma-family of pyridoxal 5'-phosphate-dependent enzymes, is present in high activity in the primitive protozoan parasite trichomonas vaginalis but is absent from mammals. two genes, mgl1 and mgl2, encoding methionine gamma-lyase, have now been isolated from t. vaginalis. they are both single copy, encode predicted proteins (mgl1 and mgl2) of 43 kda, have 69 ... | 1998 | 9488680 |
| sexually transmitted diseases in adolescents: what's new? | sexually transmitted diseases (stds) are an important cause of morbidity and, potentially, mortality for adolescents in the united states. each year, 2.5 million teenagers in this country will contract an std. an understanding of recent advances in risk factors, epidemiology, diagnosis, and treatment of these important infections is vital for clinicians providing care for adolescents. | 1998 | 9496862 |
| epidemiology and outcomes associated with moderate to heavy candida colonization during pregnancy. vaginal infections and prematurity study group. | our purpose was to determine the risk factors, physical findings, microflora, and pregnancy outcome among pregnant women with moderate to heavy vaginal growth of candida albicans and other candida species. | 1998 | 9500502 |
| diagnosis of trichomonas vaginalis in adolescent females: inpouch tv culture versus wet-mount microscopy. | this study compared the inpouch tv culture to wet-mount, diamond's culture medium, and papanicolaou (pap) smear for the diagnosis of trichomonas infection in sexually active adolescents. | 1998 | 9502007 |
| cloning and expression of an iron-containing superoxide dismutase in the parasitic protist, trichomonas vaginalis. | a superoxide dismutase (sod) gene of the parasitic protist trichomonas vaginalis was cloned, sequenced, expressed in escherichia coli, and its gene product characterized. it is an iron-containing dimeric protein with a monomeric mass of 22,067 da. southern blots analyses suggested the presence of seven iron-containing (fesod) gene copies. hydrophobic cluster analysis revealed some peculiarities in the 2d structure of the fesod from t. vaginalis and a strong structural conservation between prokar ... | 1998 | 9561738 |
| yigsr is the preferential laminin-1 residing adhesion sequence for trichomonas vaginalis. | 1998 | 9562428 | |
| clinical and microbiological aspects of trichomonas vaginalis. | trichomonas vaginalis, a parasitic protozoan, is the etiologic agent of trichomoniasis, a sexually transmitted disease (std) of worldwide importance. trichomoniasis is the most common nonviral std, and it is associated with many perinatal complications, male and female genitourinary tract infections, and an increased incidence of hiv transmission. diagnosis is difficult, since the symptoms of trichomoniasis mimic those of other stds and detection methods lack precision. although current treatmen ... | 1998 | 9564565 |
| a pilot study of metronidazole vaginal gel versus oral metronidazole for the treatment of trichomonas vaginalis vaginitis. | trichomonas vaginalis is a common sexually transmitted pathogen. in the united states, oral metronidazole is the only officially sanctioned treatment option. | 1998 | 9524997 |
| female reproductive tract immunity in bovine trichomoniasis. | mechanisms of protective immunity in the female reproductive tract are poorly understood. for sexually transmitted diseases, bovine trichomoniasis is a useful model because it resembles human trichomoniasis to some extent, and antibodies play an important role in protection against these extracellular parasites. protective efficacy was compared in animals with genital responses of predominantly immunoglobulin g (igg) or predominantly iga antibodies to a purified surface antigen of tritrichomonas ... | 1998 | 9526608 |
| collaborative approach to improve the detection and management of trichomoniasis in a low prevalence district. | we describe a simple collaborative approach developed by the departments of cytology, microbiology and genitourinary (gu) medicine for the detection, diagnosis and management of microbiologically confirmed trichomonas vaginalis (tv) infection. over a 2-year period, 54 (0.1%) of 52,440 cervical smears were reported to show tv, but microbiological confirmation was made in only 76% of 34 patients from whom a vaginal swab was subsequently taken. trichomoniasis should not be diagnosed by cytology alo ... | 1998 | 9530903 |
| a population based case-control teratologic study of oral metronidazole treatment during pregnancy. | to study human teratogenic risk of metronidazole. | 1998 | 9532994 |
| molecular analysis of the gene encoding the immunodominant phenotypically varying p270 protein of trichomonas vaginalis. | trichomonas vaginalisis a flagellated protozoan responsible for the most common non-viral sexually transmitted disease. the immunogen p270 was previously found to be up-regulated in expression and to undergo phenotypic variation between surface versus cytoplasmic localization in trichoimonads harbouring a dsrna virus. in this report, we characterize the entire p270 open reading frame (orf) and the unknown flanking 5;- and 3;-unique, non-repeat coding sequences of the gene in addition to untransl ... | 1998 | 9533894 |
| trichomonas vaginalis and amplification of hiv-1 transmission. | 1998 | 9449891 | |
| only two of the trichomonas vaginalis triplet ap51 adhesins are regulated by iron. | the sexually transmitted parasite trichomonas vaginalis cytoadheres to the vaginal epithelium, and four candidate trichomonad adhesins have been identified. one such protein, termed ap51, was characterized further. to do this, we studied a 1 kb cdna clone (ap51.2) isolated from a phagemid expression library, which encoded a fusion protein of approximately 38 kda that was immuno-crossreactive with anti-ap51 serum and retained functional adhesive properties. we performed 5'-pcr amplification to re ... | 1998 | 9466942 |
| syndromic management of sexually transmitted diseases. part 2--the management of genital discharge. | in men urethral discharge is commonly caused by neisseria gonorrhoeae and chlamydia trachomatis. both organisms cause an acute anterior urethritis and infected men usually present with symptoms of urethritis. a proportion of men with urethral infection may remain asymptomatic. amongst women the common cause of vaginal discharge is vaginitis caused by trichomonas vaginalis, candida albicans and anaerobic bacterial infection (bacterial vaginosis). however, cervicitis caused by n. gonorrhoeae and c ... | 1998 | 10101431 |
| nmr spectroscopic studies of the hydrogenosomal [2fe-2s] ferredoxin from trichomonas vaginalis: hyperfine-shifted 1h resonances. | the hyperfine-shifted 1h nmr resonances of oxidized and reduced trichomonas vaginalis ferredoxin, a functionally unique [2fe-2s] ferredoxin, have been studied. the oxidized protein spectrum displayed a pattern of six broad upfield-shifted resonances between 13 and 40 ppm with chemical shifts distinct from those of other [2fe-2s] ferredoxins. all hyperfine 1h resonances of the oxidized ferredoxin displayed anti-curie temperature dependences. reduced t. vaginalis ferredoxin displayed hyperfine res ... | 1998 | 10065531 |
| activity of disulfiram (bis(diethylthiocarbamoyl)disulphide) and ditiocarb (diethyldithiocarbamate) against metronidazole-sensitive and -resistant trichomonas vaginalis and tritrichomonas foetus. | clinical resistance of trichomonas vaginalis to metronidazole is best correlated with mic values measured under aerobic conditions. under these conditions both disulfiram (bis(diethylthiocarbamoyl)disulphide), and its first mammalian metabolite, ditiocarb (diethyldithiocarbamate), showed high levels of activity against metronidazole-sensitive (disulfiram mic, 0.1-0.7 microm; ditiocarb mic, 0.3-9 microm) and -resistant (mics 0.2-1.3 microm and 1.2-9 microm respectively) isolates. tritrichomonas f ... | 1998 | 10052908 |
| validation of a who algorithm with risk assessment for the clinical management of vaginal discharge in mwanza, tanzania. | (i) to determine the microbial aetiologies of vaginal discharge in std clinic and antenatal clinic (anc) attenders; (ii) to evaluate the performance and costs of a new who algorithm for the detection of gonococcal and chlamydial infections in women complaining of vaginal discharge and/or genital itching, using a risk assessment. | 1998 | 10023356 |
| evaluation of sexually transmitted diseases diagnostic algorithms among family planning clients in dar es salaam, tanzania. | to determine the prevalence of sexually transmitted diseases (stds) and to assess the validity of std screening approaches among family planning clients in dar es salaam, tanzania. | 1998 | 10023364 |
| risk scores to detect cervical infections in urban antenatal clinic attenders in mwanza, tanzania. | detection and management of gonococcal and/or chlamydial infections in women is a challenge, particularly in developing countries where laboratory tests are not always available. the world health organisation (who) has developed a risk assessment approach to identify cervical infections among women complaining of vaginal discharge. we have evaluated this approach as a screening strategy among women attending an urban antenatal clinic (anc) in tanzania. | 1998 | 10023365 |
| screening for sexually transmitted diseases in rural south african women. | this paper reports on a study undertaken in a rural area of south africa, to develop a non-laboratory tool to screen for sexually transmitted diseases (stds) among family planning clients. | 1998 | 10023366 |
| synthesis and biological evaluation of ureido and thioureido derivatives of 2-amino-2-deoxy-d-glucose and related aminoalcohols as n-acetyl-beta-d-hexosaminidase inhibitors. | ureido and thioureido derivatives of 2-acetamido-2-deoxy-beta-d-glucose, 1-amino-1-deoxy-d-glucitol and 2-(2-aminoethoxy)ethanol were prepared as n-acetyl-beta-d-hexosaminidase (nahase) inhibitors and were evaluated on trichomonas vaginalis nahase. although none showed complete inhibition of the enzyme at 100 microm, 1-amino-1-deoxy-d-glucitol derivatives acted as competitive inhibitors of the nahase of t. vaginalis. | 1998 | 10230037 |
| [electrophoretic analysis of soluble antigens of a pathogenic isolate of trichomonas vaginalis]. | to analyze the parasite soluble antigens of a pathogenic isolate of trichomonas vaginalis. | 1998 | 12078262 |
| using self-administered tampons to diagnose stds. | the new nucleic acid amplification techniques (e.g., lcr and pcr) have allowed the introduction of non- and minimally-invasive techniques for the detection of sexually transmitted diseases (stds). in this article, we describe one of these-the self-administrated tampon method-which we have used to detect the presence of chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis, and human papillomavirus in women. | 1998 | 11361882 |
| trichomonas vaginalis - an indicator for other sexually transmitted infecting agents. | the present study is based on 350 women having sexually transmitted diseases and 68 men counterparts. trichomonas vaginalis was a significant contributor in 216 (61.7%) out of 350 female sid cases' and in 56 (82.3%) out of 68 male counterparts. further, out of 216 cases of t.vaginalis, 41 cases (32.5%) were associated with infection with candida species; 29 (23%) with neisseria gonorrhoea. 18 cases (14.3%) with haemophilus ducreyi and 11 cases (8.7%), chlamydia trachomatis. treponema pallidum wa ... | 1998 | 20921795 |
| gene transcription in trichomonas vaginalis. | since the cloning of the first gene from the flagellated, parasitic protist trichomonas vaginalis in 1990, at least a partial sequence has been obtained from over 100 genes. molecular and biochemical analyses using these genes have enhanced our understanding of metabolism, organelle biogenesis, drug susceptibility, phylogeny and basic properties of transcription in trichomonads. here, david liston and patricia johnson discuss the available data on the regulation of transcription of protein-codin ... | 1998 | 17040780 |
| new insights into the phylogeny of trichomonads inferred from small subunit rrna sequences. | small subunit ribosomal dna sequences were obtained by polymerase chain reaction from four trichomonad species: a frog endosymbiont trichomitus batrachorum, an intestinal endosymbiont of a squamate reptile, hypotrichomonas acosta and two free-living isolates, monotrichomonas carabina and monotrichomonas sp. molecular trees inferred by distance, parsimony and likelihood techniques identify three well-resolved clusters within the trichomonads, however bootstrap values do not strongly support a par ... | 1998 | 23194718 |
| infection and cervical intraepithelial neoplasia. | the impact of sexually transmitted diseases (stds) on the development of cervical intraepithelial neoplasia (cin) has been increasingly recognized over the last 20 years. much attention has been focused on human papillomavirus (hpv) and the potential for screening for certain hpv types alongside standard cervical cytology in the hope of identifying those females at particular risk of developing high grade cin or invasive carcinoma. some infections, for example herpes simplex virus (hsv), have be ... | 1999 | 11240764 |
| sequence analysis of genes encoding ribosomal proteins of amitochondriate protists: l1 of trichomonas vaginalis and l29 of giardia lamblia. | two genes encoding the ribosomal proteins were cloned and sequenced from amitochondriate protists, l1 (l10a in mammalian nomenclature) from trichomonas vaginalis and l29 (l35 in mammalian nomenclature) from giardia lamblia. the deduced amino acid sequences were analyzed by sequence alignments and phylogenetic reconstructions. both the t. vaginalis l1 and the g. lamblia l29 displayed eukaryotic sequence features, when compared with all the homologs from the three primary kingdoms. | 1999 | 11269274 |
| preliminary studies on target antigens for the diagnosis of trichomonas vaginalis infection. | trichomonas vaginalis, a parasitic protozoa residing in the human urogenital tract, causes one of the most common sexually transmitted diseases, trichomoniasis. clinical diagnosis of t. vaginalis infection mainly involves a wet-mount microscopic examination, and a culture method, and both of which are either laborious or time-consuming. an immunodiagnostic strategy is under development, which is based on the fact that t. vaginalis releases various protein factors, notably proteinases, into the c ... | 1999 | 11561565 |
| [a clinical and microbiological study of bacterial vaginosis and vaginitis in pregnant women]. | infectious complications in pregnancy and delivery are still y serious problem in obstetrical, gynecological and neonatological practice. the main part of them are caused from the anaerobic organisms associated with bacterial vaginosis as well from sexually transmitted pathogens. in this study we present data about the frequency of spreading of bacterial vaginosis and vaginitis in pregnant women at different stages of pregnancy. we held y study of 106 pregnant women aged between 15 and 35, regis ... | 1999 | 11965718 |
| [the preoperative preparation of the vagina with betadine before abortion on demand]. | recent evidence has associated bacterial vaginosis and trichomoniasis with several postoperative complications. we carried out a prospective study aiming to estimate the frequency of vaginitis in women wanting to make an artificial abortion and the possibility to influence this infections by local application of vaginal betadine suppositories in all forms of infectious vaginitis: candida albicans, trichomonas vaginalis and bacterial vaginosis. | 1999 | 10734676 |
| [study on etiologic agents of vaginitis]. | in order to provide scientific basis for preventing and curing vaginitis, etiologic agents were examined in 354 patients with vaginitis and 115 healthy women using rapid and reliable laboratory methods. the inducements of vaginitis were analysed. the results showed that candida was only detected for the normal control group. bacteria, candida and trichomonas were the commonest causes of vaginitis. with the increasing ages of patients with vaginitis, the incidence rates of all kinds of vaginitis ... | 1999 | 11938750 |
| acetarsol pessaries in the treatment of metronidazole resistant trichomonas vaginalis. | 1999 | 12035784 | |
| [pathogen spectrum of urethritis in the man]. | 545 male patients with a tentative diagnosis "urethritis" were examined between november 1984 and december 1994 in the department of dermatology and venerology of the military hospital in ulm. the patients, aged from 18 to 58 years (mean age 24.1 years), were examined according to a standardized diagnostic procedure: smear preparations from the urethra with subsequent gram staining, bacterial cultures for aerobic bacteria, neisseria gonorrhoeae (cultures and phadebact gonococcus test), mycoplasm ... | 1999 | 10231688 |
| convergent evolution of trichomonas vaginalis lactate dehydrogenase from malate dehydrogenase. | lactate dehydrogenase (ldh) is present in the amitochondriate parasitic protist trichomonas vaginalis and some but not all other trichomonad species. the derived amino acid sequence of t. vaginalis ldh (tvldh) was found to be more closely related to the cytosolic malate dehydrogenase (mdh) of the same species than to any other ldh. a key difference between the two t. vaginalis sequences was that arg91 of mdh, known to be important in coordinating the c-4 carboxyl of oxalacetate/malate, was repla ... | 1999 | 10339579 |
| trichomonas vaginalis possesses a gene encoding the essential spliceosomal component, prp8. | 1999 | 10340492 | |
| complement-mediated regulation of trichomonas vaginalis infection in mice. | trichomonas vaginalis is a flagellated protozoan which causes trichomoniasis, a sexually transmitted disease of the human genitourinary tract. the importance of the alternative complement pathway in host defence against t. vaginalis was investigated in vitro. kinetic studies utilising immunofixation following electrophoresis showed that both a strongly and weakly virulent strain of t. vaginalis activated murine serum c3. in vivo studies with congenic-resistant, c5-deficient, b10.d2/osn- and c5-s ... | 1999 | 10343161 |
| antibiotics: treatment of preterm labor. | our intention is to review recent data and provide recommendations for the use of antibiotics in cases of preterm labor or preterm premature rupture of the membranes (pprom). various studies assessing antibiotics as treatment for preterm labor demonstrate neonatal or maternal benefits only in certain circumstances. antibiotic treatment should be given to patients with bacterial vaginosis and trichomonas vaginalis. currently, antibiotics should not be applied routinely to prolong pregnancy in wom ... | 1999 | 10343932 |
| urticaria from trichomonas vaginalis infection. | we report the case of a 32-year-old woman who had pruritic urticarial skin lesions associated with episodes of arthralgia. the first site affected by the eruption was the inside surface of the thighs; the patient also reported the presence of leukorrhea. the woman had previously been treated with h1 antagonist with moderate and transitory results; skin lesions reappeared just after the interruption of the treatment. her biochemical data showed increased levels of erythrocyte sedimentation rate, ... | 1999 | 10353101 |
| regiospecific synthesis of pyrido[3,4-b]- and pyrido[4,3-b]carbazole-5,11-dione derivatives. evaluation of their in vitro antifungal or antiprotozoological activities. | hetero diels-alder reactions between 2- or 3-bromocarbazolequinones 1a or 1b and azadiene 5 afford regiospecifically pyrido[3,4-b]- and pyrido[4,3-b]carbazole-3,5,11-triones 6a and 6b. the regiochemistry of the cycloadditions is controlled by the position of the bromine atom at c-2 or c-3 of the bromoquinone. the corresponding n- and o-methyl derivatives 7 and 8 are prepared. structural assignment of the regioisomers is made by 1h-nmr nuclear overhauser effect difference experiments performed on ... | 1999 | 10361697 |
| critical analysis of eukaryotic phylogeny: a case study based on the hsp70 family. | trichomonads, together with diplomonads and microsporidia, emerge at the base of the eukaryotic tree, on the basis of the small subunit rrna phylogeny. however, phylogenies based on protein sequences such as tubulin are markedly different with these protists emerging much later. we have investigated 70 kda heat-shock protein (hsp70), which could be a reliable phylogenetic marker. in eukaryotes, hsp70s are found in cytosol, endoplasmic reticulum, and organelles (mitochondria and chloroplasts). in ... | 1999 | 10361733 |
| delayed versus immediate bedside inoculation of culture media for diagnosis of vaginal trichomonosis. | a comparison of delayed versus immediate inoculation of culture medium for the diagnosis of trichomonosis was conducted. the sensitivities of the two methods were 100 and 97.4%, respectively. delayed inoculation of culture medium for women without evidence of trichomonosis on direct microscopic examination is a valid diagnostic procedure. | 1999 | 10364619 |
| desulfovibrio desulfuricans iron hydrogenase: the structure shows unusual coordination to an active site fe binuclear center. | many microorganisms have the ability to either oxidize molecular hydrogen to generate reducing power or to produce hydrogen in order to remove low-potential electrons. these reactions are catalyzed by two unrelated enzymes: the ni-fe hydrogenases and the fe-only hydrogenases. | 1999 | 10368269 |
| [microflora of the female genitalia and its sensitivity to antibacterial drugs]. | 1999 | 10382037 | |
| pharmacokinetics and pharmacodynamics of the nitroimidazole antimicrobials. | metronidazole, the prototype nitroimidazole antimicrobial, was originally introduced to treat trichomonas vaginalis, but is now used for the treatment of anaerobic and protozoal infections. the nitroimidazoles are bactericidal through toxic metabolites which cause dna strand breakage. resistance, both clinical and microbiological, has been described only rarely. metronidazole given orally is absorbed almost completely, with bioavailability > 90% for tablets; absorption is unaffected by infection ... | 1999 | 10384859 |
| investigation of a suspected outbreak of vaginal trichomoniasis among female inmates. | female inmates have high rates of sexually transmitted diseases (stds), and many incarcerated women and jail providers believe stds are acquired within the jail. we investigated a suspected outbreak of trichomoniasis among female inmates and described the epidemiology of trichomonas infection. goals of this study: to determine the likelihood of within-jail acquisition of trichomoniasis. | 1999 | 10417021 |