Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
cl(-) secretion in colonic epithelial cells induced by the vibrio parahaemolyticus hemolytic toxin related to thermostable direct hemolysin.a hemolytic toxin related to thermostable direct hemolysin (tdh), tdh-related hemolysin (trh), produced by kanagawa-phenomenon-negative vibrio parahaemolyticus is suspected of playing an important, but yet-to-be-elucidated role in diarrhea caused by this organism. in cultured human colonic epithelial cells, trh increases cl(-) secretion, followed by elevation of intracellular calcium.200010948178
mechanisms of chloride secretion induced by thermostable direct haemolysin of vibrio parahaemolyticus in human colonic tissue and a human intestinal epithelial cell line.thermostable direct haemolysin (tdh) produced by vibrio parahaemolyticus is thought to play an important role in the severe diarrhoea caused by this organism. this study investigated the enterotoxicity of tdh for human intestinal cells. addition of tdh to the mucosal side of human colonic tissue in ussing chambers caused increased short circuit currents (isc), a process that was inhibited by 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (dids), an inhibitor of ca2+ -activated chloride (cl- ...200010966228
characteristics of vibrio parahaemolyticus o3:k6 from asia.a variety of serovars of the food-borne pathogen vibrio parahaemolyticus normally cause infection. since 1996, the o3:k6 strains of this pathogen have caused pandemics in many asian countries, including taiwan. for a better understanding of these pandemic strains, the recently isolated clinical o3:k6 strains from india, japan, korea, and taiwan were examined in terms of pulsed-field gel electrophoresis (pfge) typing and other biological characteristics. after pfge and cluster analysis, all the o ...200010966418
reduction of endogenous bacteria associated with catfish fillets using the grovac process.fresh catfish (ictalurus punctatus) fillets are known to be contaminated with a large number of spoilage and pathogenic bacteria. the grovac method, a new patented (u.s. 5,543,163) process, was evaluated for its efficacy in reducing the number of pathogens and spoilage microorganisms associated with food. this process involves using a processing solution containing ascorbic acid (aa) and sodium chloride (nacl), vacuum, and tumbling. a total of 51 bacterial isolates were isolated and identified f ...200010983798
relation of capsular polysaccharide production and colonial cell organization to colony morphology in vibrio parahaemolyticus.vibrio parahaemolyticus is a ubiquitous, gram-negative marine bacterium that undergoes phase variation between opaque and translucent colony morphologies. the purpose of this study was to determine the factor(s) responsible for the opaque and translucent phenotypes and to examine cell organization within both colony types. examination of thin sections of ruthenium red-stained bacterial cells by electron microscopy revealed a thick, electron-dense layer surrounding the opaque cells that was absen ...200010986256
antibiotic- and metal-resistant strains of vibrio parahaemolyticus isolated from shrimps.enteropathogenic strains of vibrio parahaemolyticus were isolated from shrimps, penaeus monodon collected from the region of the deltaic sundarbans (west bengal, india). about 63% of the isolated strains were resistant to ampicillin, cephalexin, and kanamycin. however, all these strains were sensitive to nitrofurantoin, nalidixic acid, tetracycline, and norfloxacin. the isolated strains were resistant to ni2+] (75%), cu2+ (87%), and co2+ (37%), but all the strains were resistant against cd2+, zn ...200010990273
genetic characterization of dna region containing the trh and ure genes of vibrio parahaemolyticus.we have demonstrated that possession of the gene for thermostable direct hemolysin-related hemolysin (trh) coincides with the presence of the urease gene among clinical vibrio parahaemolyticus strains and that the location of the two genes are in close proximity on the chromosome. here, we cloned and sequenced the 15,754-bp dna region containing the trh gene and the gene cluster for urease production from the chromosome of clinical v. parahaemolyticus (th3996). we found 16 open reading frames (o ...200010992480
emergence of a new vibrio parahaemolyticus serotype in raw oysters: a prevention quandary.in may and june 1998, reported vibrio parahaemolyticus infections increased sharply in texas.200011000648
a novel action of the proton pump inhibitor rabeprazole and its thioether derivative against the motility of helicobacter pylori.the motility of helicobacter pylori was maximum at 37 degrees c and at ph 6. a newly developed proton pump inhibitor, rabeprazole (rpz), and its thioether derivative (rpz-th) markedly inhibited the motility of h. pylori. the concentrations of the drug necessary to inhibit 50% of the motility were 0.25, 16, 16, and >64 microgram/ml for rpz-th, rpz, lansoprazole, and omeprazole, respectively. no such inhibitory effects were observed with h(2) blockers or anti-h. pylori agents. the motilities of ca ...200011036024
structural variation in the 16s-23s rrna intergenic spacers of vibrio parahaemolyticus.the 16s-23s rrna intergenic spacers (igs) of vibrio parahaemolyticus were pcr-amplified and cloned with pt7blue t vector. a total of six clones were isolated dependent on size difference. the clones were different with respect to both the number and the composition of the trna genes included, and were designated igs-0, igs-e, igs-ia, igs-ae, igs-ekv and igs-ekav. igs-ekav included the cluster of trna(glu)-trna(lys)-trna(ala)-trna(val); igs-ekv, trna(glu)-trna(lys)-trna(val); igs-ae, trna(ala)-tr ...200011040431
environmental investigations of vibrio parahaemolyticus in oysters after outbreaks in washington, texas, and new york (1997 and 1998).total vibrio parahaemolyticus densities and the occurrence of pathogenic strains in shellfish were determined following outbreaks in washington, texas, and new york. recently developed nonradioactive dna probes were utilized for the first time for direct enumeration of v. parahaemolyticus in environmental shellfish samples. v. parahaemolyticus was prevalent in oysters from puget sound, wash.; galveston bay, tex.; and long island sound, n.y., in the weeks following shellfish-associated outbreaks ...200011055906
clonal dissemination of vibrio parahaemolyticus displaying similar dna fingerprint but belonging to two different serovars (o3:k6 and o4:k68) in thailand and india.active surveillance of vibrio parahaemolyticus infection among hospitalized patients in calcutta, india, showed the appearance of the o4:k68 serovar for the first time in march 1998 alongside the continued predominant incidence of the o3:k6 serovar. strains belonging to both these serovars have been reported to possess pandemic potential. the genomes of o3:k6 and o4:k68 strains and for comparison, non-o3:k6 and non-o4:k68 strains isolated from two different countries, india and thailand, were ex ...200011057955
a unique and common restriction fragment pattern of the nucleotide sequences homologous to the genome of vf33, a filamentous bacteriophage, in pandemic strains of vibrio parahaemolyticus o3:k6 o4:k68, and o1:k untypeable.vf33 is a filamentous bacteriophage isolated from vibrio parahaemolyticus. we performed southern blot hybridization analysis to examine the distribution of vf33-related genetic elements in the pandemic strains (o3:k6 strains isolated between 1995 and 1997, o4:k68 and o1:k untypeable strains isolated between 1997 and 1999) of v. parahaemolyticus. nucleotide sequences homologous to the vf33 dna were detected in all 57 test strains including pandemic and non-pandemic strains. however, the profiles ...200011064200
norm of vibrio parahaemolyticus is an na(+)-driven multidrug efflux pump.norm of vibrio parahaemolyticus apparently is a new type of multidrug efflux protein, with no significant sequence similarity to any known transport proteins. based on the following experimental results, we conclude that norm is an na(+)-driven na(+)/drug antiporter. (i) energy-dependent ethidium efflux from cells possessing norm was observed in the presence of na(+) but not of k(+). (ii) an artificially imposed, inwardly directed na(+) gradient elicited ethidium efflux from cells. (iii) the add ...200011073914
molecular evidence of clonal vibrio parahaemolyticus pandemic strains.the upsurge in worldwide incidence of vibrio parahaemolyticus infection in the last 5 years has been attributed to the recent appearance of three serotypes with pandemic potential: o3:k6, o4:k68, and o1:k untypeable (kut). thirty-five strains of these serotypes, isolated from different countries over 4 years, were characterized by ribotyping and pulsed-field gel electrophoresis to determine their origin. the ribotypes of the strains of these serotypes were indistinguishable, except for a japanes ...200011076722
phylogeny of vibrio cholerae based on reca sequence.we sequenced a 705-bp fragment of the reca gene from 113 vibrio cholerae strains and closely related species. one hundred eighty-seven nucleotides were phylogenetically informative, 55 were phylogenetically uninformative, and 463 were invariant. not unexpectedly, vibrio parahaemolyticus and vibrio vulnificus strains formed out-groups; we also identified isolates which resembled v. cholerae biochemically but which did not cluster with v. cholerae. in many instances, v. cholerae serogroup designat ...200011083852
production of serine protease of aeromonas sobria is controlled by the protein encoded by the gene lying adjacent to the 3' end of the protease gene.we cloned a protease gene of aeromonas sobria and determined its nucleotide sequence. the protease is composed of 624 amino acid residues and its calculated molecular weight is 66,737.7. the amino acid sequence showed the characteristic features of a bacterial serine protease. we expressed the protease gene in vibrio parahaemolyticus from which the synthesized protease is secreted into the culture medium as the mature form, and purified the mature protease by successive column chromatographies. ...200011092244
vibrio parahaemolyticus serovar o3:k6 as cause of unusually high incidence of food-borne disease outbreaks in taiwan from 1996 to 1999.the occurrence of food-borne disease outbreaks in taiwan increased dramatically in 1996, and the incidence has since remained elevated. this increase in outbreaks is correlated with a high rate of isolation of vibrio parahaemolyticus, which caused between 61 and 71% of the total outbreaks for the period 1996 to 1999. by serotyping, 40 serovars were identified from 3743 v. parahaemolyticus isolates, of which o3:k6 was the most frequently detected. the o3:k6 serovar could have emerged in taiwan as ...200011101606
virulence of vibrio parahaemolyticus isolated from cultured small abalone, haliotis diversicolor supertexta, with withering syndrome.outbreaks of mass mortality among cultured small abalone haliotis diversicolor supertexta with withering syndrome occurred in may and september 1998 in kao-hsiung, taiwan. bacterial strains ch-1 and b4 were isolated from the haemolymph of the moribund small abalone using tryptic soy agar supplemented with 3% nacl and/or thiosulphate citrate bile salt sucrose agar. these two strains were characterized and identified as vibrio parahaemolyticus on the basis of various biochemical tests. the b4 stra ...200011123551
characterization of the pili isolated from vibrio parahaemolyticus o3:k6.pilus of vibrio parahaemolyticus o3:k6 strain lvp9 belonging to the newly identified clone was purified and characterized. the molecular mass of the pilin was estimated to be about 18 kda by sds-page, and the isoelectric point of the pilin was 5.0 +/- 0.2. the lvp9 pili were antigenically different from the other v. parahaemolyticus na2 pili and ha7 pili as previously reported, nevertheless all three had indistinguishable morphology and shared a high degree of homology in their n-terminal amino ...200011127340
metal content and biochemical analyses of a recombinant collagenase prtv from vibrio parahaemolyticus.prtv is an extracellular metalloprotease of vibrio parahaemolyticus and regarded as a collagenase. inductively coupled plasma-optical emission spectrometry analysis indicated that the recombinant prtv contains 1 mol of zinc per mol of the native enzyme. on the basis of a kinetic study using 2-furanacryloyl-leu-gly-pro-ala (falgpa, the specific substrate for bacterial collagenase) as a substrate, it was suggested that metal ions may play a significant role in the binding and catalytic steps of th ...200011128063
evaluation of nonisotopic dna hybridization methods for detection of the tdh gene of vibrio parahaemolyticus.production of the thermostable direct hemolysin (tdh) by vibrio parahaemolyticus is associated with pathogenicity of the organism and is encoded by the tdh gene. the timely resolution of seafood-associated outbreaks requires rapid and accurate detection of pathogenic v. parahaemolyticus. the specificity of alkaline phosphatase- and digoxigenin-labeled tdh gene probes was evaluated against 61 strains of v. parahaemolyticus (including isolates from recent outbreaks involving oysters from the pacif ...200011131887
characterization of a novel vibrio parahaemolyticus phage, kvp241, and its relatives frequently isolated from seawater.a vibriophage, kvp241, and six of its relatives were isolated independently from seawater using vibrio parahaemolyticus as the host. all of the phages had the same morphology (a hexagonal head and a tail with a contractile sheath) and the same host range (specific for some v. parahaemolyticus strains). dna-dna hybridization experiments elucidated that their genomes are highly homologous to each other. analyses of amino acid sequences of putative major capsid proteins indicated that kvp241 may be ...200011145278
detection of bacterial pathogens by phage antibody display. 200011360836
distribution of vibrio cholerae virulence genes among different vibrio species isolated in sardinia, italy.the members of the genus vibrio include harmless aquatic strains as well as strains capable of causing epidemics of cholera. diarrhoea caused by vibrio cholerae is attributed to cholerae enterotoxin (ct) codified by the ctx operon and regulated by a number of virulence genes such as toxt, toxr and toxs. fifty-two vibrio strains were isolated from different aquatic environments in and around sardinia and searched by pcr for the presence of ctxa, zot, ace, toxr, toxs, toxt, tcpa and vpi virulence ...200010747228
effect of mg(2+) ion in protein secretion by magnesium-resistant strains of pseudomonas aeruginosa and vibrio parahaemolyticus isolated from the coastal water of haldia port.a rapidly growing industrial complex including oil refineries and chemical industries has developed around the coastal area of haldia port in the district of midnapore, west bengal, india. the coastal water is highly polluted with industrial wastes along with petroleum hydrocarbons. the bacteria isolated from the different sites of the coastal waters were escherichia coli, alcaligenes, acinetobacter, klebsiella spp., micrococcus spp., vibrio spp., pseudomonas aeruginosa and vibrio parahaemolytic ...200010754240
proteomics on full-length membrane proteins using mass spectrometry.a general technique has been developed that allows rapid mass spectrometric analysis of full-length membrane proteins [whitelegge, j. p., le coutre, j., et al. (1999) proc. natl. acad. sci. u.s.a. 96, 10695-10698]. using in-line hplc electrospray ionization mass spectrometry (lc-ms), different native and recombinant bacterial membrane proteins of up to 61 kda are characterized. mass spectrometric data of four entirely different membrane proteins from three bacterial organisms, two transporters, ...200010757971
resuscitation of viable but nonculturable cells of vibrio parahaemolyticus induced at low temperature under starvation.vibrio parahaemolyticus is known to exist in a viable but nonculturable state when incubated at low temperature under starvation. it has long been debated whether the culturable cells which appear after temperature upshift are the result of true resuscitation or regrowth of a few residual culturable cells. starved v. parahaemolyticus cells at 4 degrees c reached the nonculturable stage in about 12 days. the true resuscitation of nonculturable cells of v. parahaemolyticus occurred after spreading ...200010779722
nhaa, an na(+)/h(+) antiporter involved in environmental survival of vibrio cholerae.vibrio cholerae, the agent of cholera, is a normal inhabitant of aquatic environments, in which it survives under a wide range of conditions of ph and salinity. in this work, we identified the nhaa gene in a wild-type epidemic strain of v. cholerae o1. nhaa encodes a protein of 382 amino acids that is very similar to the proteins nhaa of vibrio parahaemolyticus, vibrio alginolyticus ( approximately 87% identity), and escherichia coli (56% identity). v. cholerae nhaa complements an e. coli nhaa m ...200010781565
the marine pathogen vibrio vulnificus encodes a putative homologue of the vibrio harveyi regulatory gene, luxr: a genetic and phylogenetic comparison.vibrio vulnificus is an opportunistic pathogen that exhibits numerous virulence factors, including the secretion of a zinc metalloprotease and the production of a capsule. we have cloned and sequenced a gene from v. vulnificus that is a homologue of the positive transcriptional regulator, luxr, of the lux operon in vibrio harveyi. this gene encodes a putative, single complete open reading frame designated smcr, which shares greater than 75% nucleotide identity with luxr of v. harveyi. the deduce ...200010806366
application of pulsed-field gel electrophoresis to the investigation of a nosocomial outbreak of vibrio parahaemolyticus.the method of pulsed-field gel electrophoresis (pfge) for vibrio parahaemolyticus was first published in 1996. since then, its application has been seldom reported in the literature. a food poisoning outbreak due to v. parahaemolyticus occurred in five wards of a hospital was investigated using this method. twenty-five patients were involved and all of them had eaten food supplied by the hospital. of the 15 cases whose stools were available for culture, only four cultures yielded v. parahaemolyt ...200010806961
insertional inactivation of genes encoding components of the sodium-type flagellar motor and switch of vibrio parahaemolyticus.vibrio parahaemolyticus possesses two types of flagella, polar and lateral, powered by distinct energy sources, which are derived from the sodium and proton motive forces, respectively. although proton-powered flagella in escherichia coli and salmonella enterica serovar typhimurium have been extensively studied, the mechanism of torque generation is still not understood. molecular knowledge of the structure of the sodium-driven motor is only now being developed. in this work, we identify the swi ...200010648530
pandemic spread of an o3:k6 clone of vibrio parahaemolyticus and emergence of related strains evidenced by arbitrarily primed pcr and toxrs sequence analyses.vibrio parahaemolyticus o3:k6 strains responsible for the increase in the number of cases of diarrhea in calcutta, india, beginning in february 1996 and those isolated from southeast asian travelers beginning in 1995 were shown to belong to a unique clone characterized by possession of the tdh gene but not the trh gene and by unique arbitrarily primed pcr (ap-pcr) profiles (j. okuda, m. ishibashi, e. hayakawa, t. nishino, y. takeda, a. k. mukhopadhyay, s. garg, s. k. bhattacharya, g. b. nair, an ...200010655349
can the family physician avoid conflict of interest in the gatekeeper role? an affirmative view. 20002723596
[growth and development of children with cerebral palsy and related nursing action. 4. practice of assistance for the child. 1]. 200097455
adduct formation between the carcinogen n-acetoxy-2-acetylaminofluorene and synthetic polydeoxyribonucleotides.the chemical carcinogen n-acetoxy-2-acetylaminofluorene (na-aaf) was reacted with poly(dg-dc) - poly(dg-dc); poly dg - poly dc; poly(da-dt) - poly (da-dt); and poly da - poly dt under a variety of conditions. poly (dg-homo gc polymer and 10--20 more reactive the a + t polymers. lowering the ionic strength increased the extent of reaction, while ph change (8.9 vs. 5.5) had only a small effect. if ionic strength was adjusted so that the two guanine-containing polymers showed equal thermal stabilit ...200018291
[pathways to new antibiotics]. 2000101812
[therapy with beta adrenergic agents]. 20002869565
notes on the spectrum of opiate use. 200097557
characterization of fractional mutations in drosophila: role of rna, protein & repair synthesis. 200094307
[economic crisis in public health in the capitalist countries]. 200097790
[therapy of cronh's disease in the acute stage, using ornidazole (tiberal). preliminary results].9 patients with acute exacerbations of crohn's disease were treated over 2.5 months or longer with ornidazole (nitroimidazole). while 3 patients showed no response at all to 5 cycles of ornidazole treatment, 6 patients improved markedly within 2-3 months. in all but 1 patient, the improvement lasted during long-term therapy (average 9 months). therapy was discontinued in 5 of these patients after 2.5-14 months; 2 patients had severe recurrences within 2-10 weeks, while 3 patients remained well d ...200097778
hypertensive complications in patients with aldosterone-producing adenomas. 200087647
early detection of cancer of oesophagus along caspian littoral. report of a pilot project.a simple mass-screening procedure for the early detection by brush cytology of oesophageal cancer in 280 adults in high-incidence villages of the caspian littoral is described. the presence of atypical cells in 7%, and the discovery of frank carcinoma in 3 symptom-free people encourages continuation of the project.200087555
[treatment of pain with psychotropic drugs (author's transl)].30 patients suffering from therapy-resistant chronic pain were divided into three groups according to drive and mood: i. psychomotor inhibition combined with depressive mood, ii. agitation and depressive mood, iii. agitation combined normal mood. the psychopharmacological treatment of pain showed sufficient results, when the drugs were directed to the target symptoms "agitation", "lack of drive", and "depression". the differentiated administration of activating or sedative antidepressants and ne ...200027829
clinical trial of topical disodium chromoglycate in vernal keratoconjunctivitis.the clinical picture of spring catarrh was studied in 96 cases. the duration of the disease was found to be 3.5 +/- 2.6 years. it exists all the year round with a higher frequency between april and august. male children were affected about three times more frequently than females. the effect of dscg was studied in 20 cases. it had a significant therapeutic effect. we advise a combined dscg and corticosteroid treatment in the period of exacerbation.2000101923
lack of uniformity in colour matching.1. the fraction of red in a red--green mixture matched to yellow increased as the intensities of the match constituents were increased sufficiently to bleach appreciable chlorolabe and erythrolabe. 2. all changes in matching found for a given normal trichromat, (i) with increase in the intensities of the matching components, (ii) as a function of time after the onset of very intense components, (iii) with change in the pupil region through which light enters the eye, and (iv) with change in the ...2000313984
[arrhythmias in direct fetal electrocardiography]. 200092107
genital herpes zoster: a case resembling primary herpes progenitalis with distal urethral involvement. 2000101907
[t-lymphocytes in protein-calorie malnutrition in infants]. 2000101962
in vitro analysis of allogeneic lymphocyte interaction. iv. dual recognition of b cell-associated mls locus and i-region determinants by a helper allogeneic effect factor (aef) generated across a minor h locus disparity.a helper allogeneic effect factor (aef) was produced across an incompatibility at the minor histocompatibility loci. this aef is genetically restricted in its activity since it helps b cells only of the stimulator haplotype and of haplotypes that share both an mls and i-region identity with the stimulator haplotype. the i-region genes involved here map to the i-a and/or i-c subregions. an anti-lym immunoadsorbent column but neither an anti-h-2 nor an anti-ia column absorbed aef helper activity. ...200087470
the production of hydroxyl radical by tallysomycin and copper(ii). 200087348
the nursing care and management of coronary artery grafts (revised). 200087189
radiation biophysical studies with mammalian cells and a modulated carbon ion beam. 200097728
the ultrastructure of cell division in euglena gracilis.the ultrastructure of mitosis in euglena gracilis was investigated. at preprophase the nucleus migrates anteriorly and associates with the basal bodies. flagella and basal bodies replicate at preprophase. cells retain motility throughout division. the reservoir and the prophase nucleus elongate perpendicular to the incipient cleavage furrow. one basal body pair surrounded by a ribosome-free zone is found at each of the nuclear poles. the spindle forms within the intact nuclear envelope- polar fe ...200097303
frontiers of quantitative cytochemistry: a review of recent developments and potentials.this article attempts to describe the current state of quantitative cytochemical methods and to point out areas where, in the author's opinion, the greatest progress is either being made or can be expected. nearly 300 references to the current literature are cited.200094776
long term survival following firearm injury to the head. 2000110996
hla antigens in a psoriatic family: comparative studies with mlc, htc, plt and serological hla-dr determinations.hla genotypes were characterized in a large family of 48 individuals in three generations. in this family, carriers of the proband's disease-predisposing haplotype commonly expressed clinical signs of illness, manifested as psoriasis and/or arthritic lesions. from these data, and from other family studies, presented previously, we have concluded that cutaneous and/or joint lesions may be signs of disease in carriers of the predisposing hla haplotype. we have investigated hla-a, b, c and d/dr ant ...200094702
toward the future in educational systems. 200092186
results of cytostatic therapy of metastasizing testicular tumors. 2000111320
the use of acupuncture needles as sphenoidal electrodes in electroencephalography: a reappraisal. 2000110550
mesonephric duct remnants (gartner's duct).in a review of 820 hysterosalpingograms, unilateral opacification of the mesonephric duct (gartner's duct) was observed in five individuals. no associated anomalies of the genitourinary tract or definite evidence of pelvic inflammatory disease was found in any of these patients. the embryology of the genitourinary tract is discussed in some detail, and histologic studies of the uterine cervix are reviewed to explain the radiographic observations.200098997
beta thromboglobulin and glycosylated haemoglobin in diabetes mellitus.no correlation has been demonstrated between the elevated levels of plasma beta-thromboglobulin and glycosylated haemoglobin found in a group of 97 diabetic patients. the abnormality in diabetic platelets which is reflected by beta-thromboglobulin levels may not therefore be a direct result of poor metabolic control. the highest levels of beta-thromboglobulin were found in those patients with a raised blood urea. glycosylated haemoglobin levels correlated with prevailing blood glucose and with t ...200094458
[effect of phosphoinositides on the structure and permeability of bilayer phospholipid vesicles]. 200092396
the effect of native and sonicated double-stranded polyribonucleotides on the course of spontaneous autoimmune disease in nzb and nzb/swiss f1 mice.the administration of sonicated fractions of f2 phage polyribonucleotides caused an increased weight loss and deterioration of the clinical state in female nzb mice. discontinuance of the treatment resulted in an improvement of both the clinical state and the genetically determined autoimmune disorders of these mice. some potential explanations of this effect are discussed.200094768
[the 38th congress of the japan neurosurgical society. abstracts]. 200094653
debugging the system. 200088713
reduced plasma viscosity among joggers compared with non-joggers. 200092344
use of the fluorochrome 4'6-diamidino-2-phenylindole in genetic and developmental studies of chloroplast dna.use of the dna-specific fluorochrome 4'6-diamidino-2-phenylindole (dapi) makes it possible to examine in situ the structure of chloroplast dna (chdna) with the fluorescence microscope. this simplifies the study of genetic and developmental changes in chloroplast dna. three examples are presented. (a) wild-type euglena gracilis b contains several chloroplast dna nucleoids per chloroplast. a yellow mutant lacking functional chloroplasts is similar, but such nucleoids are absent in an aplastidic mu ...200090051
interaction of alcohol and reward in an achievement situation.effects of psychological stress on acute alcohol intoxication following intake of 2 ml whisky/kg body weight were examined in 12 healthy subjects. each subject performed a 45-min psychomotor task under four conditions, two of which increased achievement stress by promising the subject a monetary reward for rapid and accurate performance. the conditions were (a) task only, (b) task + reward, (c) task + alcohol, and (d) task + reward + alcohol. in condition (b) reward improved performance and heig ...2000111286
biliary duct ultrasound. 200099016
colitis cystica profunda. 200099009
drug-induced lung disease.since there are no diagnostic studies to confirm the presence of a drug-induced lung reaction the physician will make a correct diagnosis only if he is aware of the drugs which have been identified to cause pulmonary reactions and their specific manifestations. failure to recognize a drug-induced lung disease can lead to significant morbidity and in some cases mortality. the major drug-induced lung diseases are reviewed, the drugs being presented in the context of their clinical use and the reac ...2000102005
deafness due to hypervitaminosis d. 200087663
esophagogastrectomy for carcinoma of the middle third of the esophagus.elective esophagogastrectomy and reconstruction by esophagogastrostomy were performed on 55 patients with malignant tumors of the midesophagus, despite invasion of contiguous structures in 60% and regional lymph node involvement in 75%. the operations were invariably palliative. two patients died within thirty days of operation. dysphagia was relieved and oral alimentation resumed in the other 53. twenty-nine patients who had experienced painful swallowing and 16 who had vomiting obtained relief ...200092220
loin pain/haematuria syndrome. 200086752
[a life with psychiatric patients. 8. a patient who wanted to see the blue sky]. 200097451
detection of specific rnas or specific fragments of dna by fractionation in gels and transfer to diazobenzyloxymethyl paper. 200094421
steroids in asthma. 200091910
severe skin pain after puva treatment.severe skin pain lasting one or two months occurred in 8 of 210 patients treated with puva. the pain started 4--8 weeks after the initial dose, mostly about one week after discontinuation of the treatment. it was a prickling, burning pain, usually coming in bouts and confined to limited areas "deep under the skin". in some respects the pain was related to itching, but the patients could easily distinguish between the two sensations. a variety of drugs was tried, but none had any noteworthy effec ...200193376
training of doctors in developing countries.developing areas like bangladesh continue to suffer from the discriminatory provision of health care. health care is controlled by urban elites to the detriment of the poor urban masses as well as the rural population as a whole. that is to say, health care in most developing countries is urban oriented and class structured. it is not so much a question of improving efficiency as of restructuring power relationships, of which health care is but one part. in bangladesh at the end of 1971, approxi ...200190840
peripheral neuropathy in a spray-painter. 200190835
complications associated with aphakic contact lenses.complications associated with rigid contact lenses are more frequent in aphakia. the limited dexterity of older patients not only may prove an insurmountable barrier to the mastery of daily contact lens insertion and removal, but also may produce significant direct eye trauma, its seriousness determined by the integrity of the cataract wound. since prolonged wear of aphakic contact lenses is the rule rather than the exception, the risk of irreversible corneal changes such as vascularization and ...200193260
[changes in physicochemical parameters and coagulation factors in hemodilution with dextran of molecular weight 60,000].in 18 subjects undergoing aorto-bifemoral by-pass, pre-operative isovalaemic hemodilution was carried out (using a dextran of molecular weight 60,000) at the same time as venous section of a mean value of 1087 ml reducing the hematocrit to 0.30. the following were studied before, immediately after and 24 hours after hemodilution: blood viscosity (at variable shearing rates), oncotic pressure, sedimentation rate, clotting factors, plasma electrolytes. these results showed that at the end of hemod ...200195471
the lorica of dinobryon. 200190737
alpha1-fetoprotein measurement in blood spotted on paper: discriminating test for hereditary tyrosinemia in neonatal mass screening.we describe an electroimmunodiffusion technique for measuring alpha1-fetoprotein in blood spotted on chromatography paper. the system is being used as a complementary test in a neonatal mass-screening program for detection of inborn metabolic diseases in the province of quebec. in a series of 102 cases of neonatal hypertyrosinemia, the test has proven to be highly discriminative for hereditary tyrosinemia. it has permitted early detection of eight cases of this disease, including two that would ...200158743
[medical management of tachycardias (author's transl)].guidelines for a step-wise plan of treatment of tachycardias have been compiled based on clinical empirical experience and with the aid of surface electrocardiograms, intracardial electrograms and stimulation techniques. the plan is primarily with the aid of surface electrocardiograms, intracardial electrograms and stimulation techniques. the plan is primarily oriented with respect to the antiarrhythmic efficacy, the adverse reactions and the practicability of the respective agents. any type of ...200193075
[psychological diagnosis problems in children between 0 and 3 years of age]. 200195256
[biochemical and coagulation-physiology examinations before and after amniocentesis in early pregnancy].in 46 women the alpha1-fetoprotein content, the soluble fibrin monomer complexes (lfmk), and the coagulation status were determined in the maternal serum at the beginning of the second trimester before and after amniocentesis. increases of afp by more than 100% of the original level pointed to fetomaternal microtransfusion, the quantity of which was calculated by means of a formula. overall coagulation tests did not show any change after the puncture. a moderate lfmk increase was seen (p less th ...200190435
[questions to television from psychopathology].progress is expected in psychopathological diagnosis by using audiovisual procedures. nowadays, a valid psychopathology concept should be broadly planned and the important method of description as well as understanding procedures should be considered. for such a model of psychopathology it seems appropriate to investigate the different psychopathological phenomenons as expression of a disturbed communication ability. concerning this outlook, new strategies are to be developed or procedures of ot ...200190381
visual aids in nutrition education. a guide to their preparation and use. 200195011
differential inhibition of prostacyclin production and platelet aggregation by aspirin. 200192623
[volvulus of the afferent loop after billroth ii operation (author's transl)].a case report is given of a patient, who had a volvulus of the afferent loop after billroth ii operation; on relaparotomy it was found, that this loop had slipped cranially through the mesocolic slit, twisted, and become gangreneous, due to bad adjustement of the slit. increased levels of serum amylase initially had given rise to the suspicion, that the deteriorating condition of the patient was due to pancreatitis. the patient recovered fully after resection had been done and an y-roux-anastomo ...200194934
[combination radio and chemotherapy in plasmocytoma. roentgen diagnostic and protein chemistry development prognosis]. 200195496
tumor-specific immunity induced by somatic hybrids. ii. elicitation of enhanced immunity against the parent plasmacytoma.hybrid cells derived from fusion of a balb/c plasmacytoma (tepc-15) and l cells (c3h origin) were used to stimulate tumor-specific immunity against the parental plasmacytoma cells. live hybrid cells induced tumor-specific immunity against tepc-15 more effectively than mitomycin-treated hybrid or tepc-15 tumor cells. adoptive transfer of immunity with spleen cells of mice immunized with hybrid cells was also more effective than that with mitomycin-treated tumor cells. the immunity induced by the ...200188483
quantitative succinate-dehydrogenase histochemistry. iii. variations in histochemical succinatedehydrogenase activity in different cross-sections of the same muscle fibre.the variation in histochemical sdh-activity at different levels in the same muscle fibre was determined in muscle fibre cross-sections both by visual classification and quantitative determination of the formazan-deposits. this work resulted in a confirmation of the earlier micro-biochemical studies of spamer and pette (1977, 1979) and lowrey et al. (1978) that the activity of enzymes of the citric acid cycle is not homogeneously distributed in a muscle fibre over its entire length. in addition i ...200193101
lifestyle adjustments in elderly patients after burn injury.although survival of older burn victims has improved significantly in recent years, controversy remains over the care that should be given to this group and the functional recovery of survivors. to assess the impact of burn injury on lifestyle in the elderly, we conducted a mail-in survey among surviving patients ages 45 years or older who were treated in our burn center from 1978 to the middle of 1987. the survey asked questions about living arrangements, personal care, family relations, social ...20011572857
perinatal mortality. 200193220
haploid cell cultures of drosophila melanogaster. 200198714
family life cycle resource center in the elementary school. 200198495
Refresh
firstprev
...21222324252627282930...
nextlast
Displaying items 2401 - 2500 of 6009