Publications

cross-reactivity of a monoclonal antibody directed against an estrogen-induced protein in mcf-7 human breast cancer cells with murine leydig cell tumor proteins.	an estrogen-responsive murine leydig cell tumor (m5480a) was examined for the presence of cross-reactive proteins to a monoclonal antibody directed against a mr 24,000 estrogen-regulated protein in human breast cancer cells. human breast tumor biopsies were used as controls for the cytosol preparations, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and western blot conditions used in these experiments. the estrogen-regulated mr 24,000 protein was detected in sodium dodecyl sulfate-p ...	1986	3940199
the addition of nitrous oxide to halothane decreases renal and splanchnic flow and increases cerebral blood flow in rats.	thirteen male sprague-dawley rats were anaesthetized with halothane and catheters were placed in the femoral artery and left ventricle. the animals breathed spontaneously through a tracheostomy throughout the investigation. after the surgical preparation, the inspired halothane concentration was maintained at 1.5 vol% (f/o2 = 0.3). after a 30-min stabilization period, strontium-85 radiolabelled microspheres (15 +/- 1 micron) were injected to the left ventricle for determination of cardiac output ...	1986	3942673
the addition of nitrous oxide to halothane decreases renal and splanchnic flow and increases cerebral blood flow in rats.	thirteen male sprague-dawley rats were anaesthetized with halothane and catheters were placed in the femoral artery and left ventricle. the animals breathed spontaneously through a tracheostomy throughout the investigation. after the surgical preparation, the inspired halothane concentration was maintained at 1.5 vol% (f/o2 = 0.3). after a 30-min stabilization period, strontium-85 radiolabelled microspheres (15 +/- 1 micron) were injected to the left ventricle for determination of cardiac output ...	1986	3942673
fate of n-(2-hydroxypropyl)methacrylamide copolymers with pendent galactosamine residues after intravenous administration to rats.	n-(2-hydroxypropyl)methacrylamide (hpma) copolymers bearing galactosamine residues accumulate in the liver after intravenous administration to rats (duncan, r., kopecek, j., rejmanová, p. and lloyd, j.b. (1983) biochim. biophys. acta 755, 518-521). in this study hpma copolymers bearing pendent galactosamine residues (1.0-11.6 mol%) were injected intravenously into rats and their rates of blood clearance and liver accumulation were measured. a level of substitution of 4 mol% was found to be suffi ...	1986	3942780
temperature-controlled plasmid regulon associated with low calcium response in yersinia pestis.	both the low calcium response and virulence in yersinia pestis strain kim5 are mediated by genes located on the 75.4-kilobase plasmid pcd1. the results presented here demonstrate the existence of two new genetic loci of pcd1 whose expression is regulated in response to temperature. levels of transcription in the trta and trtb loci were elevated 12- to 16-fold at 37 degrees c compared with levels of transcription at 30 degrees c. in addition, the absolute levels of transcription were the highest ...	1986	3944056
septicemic plague in a 14-month-old child.		1986	3945561
plasmid-determined cytotoxicity in yersinia pestis and yersinia pseudotuberculosis.	yersinia pestis kim5 was found to be cytotoxic for the ic21 and p388d1 mouse macrophage cell lines, as well as for resident peritoneal macrophages from c57bl/6 mice. affected cells phagocytosed kim5 inefficiently, became spherical, detached readily from culture dishes, and retained 51cr poorly. the cytotoxic effect was dependent on the presence of the 75-kilobase plasmid pcd1. because this plasmid also encodes the low calcium response (lcr), three mu d1 insertion mutants previously shown to be l ...	1986	3949380
binding of 4(5)-[2-(4-azido-2-nitroanilino)ethyl]imidazole to histamine receptors in guinea pig cerebral cortical membranes.	the interaction of 4(5)-[2-(4-azido-2-nitroanilino)ethyl]imidazole (aah), a photolabile histamine receptor antagonist, with the binding of histamine, mepyramine, and tiotidine to guinea pig cerebral cortical membranes was examined to evaluate the specificity of aah for histamine h1 and h2 receptors. saturable, specific binding of [3h]histamine, [3h]mepyramine, and [3h]tiotidine to the membranes was observed. competition assays were used to assess the relative affinity of aah for h1- and h2-recep ...	1986	3952080
international control of smoking and the us experience.	international data demonstrate convincingly that lung cancer death rates follow and parallel cigarette smoking prevalence rates in both men and women. effective control of lung cancer and the many other diseases associated with smoking can be achieved only by continued reductions in smoking prevalence in developed countries and the arrestment of smoking in developing countries. smoking control strategies can take the form of prevention programs, cessation programs, regulatory and legislative mea ...	1986	3956298
does the pattern of tissue mobilization dictate protein requirements?		1986	3957715
bubonic plague in a cat.	bubonic plague was diagnosed in a cat. the diagnosis was made by isolation of bacteria from a ruptured bubo and by demonstration of an antibody titer to yersinia pestis. the cat was treated successfully with amoxicillin and streptomycin. the entire veterinary staff was treated prophylactically with tetracycline.	1986	3957763
nucleotide sequence of the enterotoxin b gene from staphylococcus aureus.	the complete nucleotide sequence of the enterotoxin b gene from staphylococcus aureus, as well as the 5' and 3' flanking regions, was determined. starting from an atg initiator codon, an open reading frame encoded the enterotoxin b precursor that consisted of 266 amino acids (mr, 31,400). the 5' terminal portion of the gene encodes a signal peptide 27 amino acids long. the deduced amino acid sequence matched, with a few exceptions, the published amino acid sequence of enterotoxin b. the structur ...	1986	3957869
retinal constraints on orientation specificity in cat visual cortex.	most retinal ganglion cells (levick and thibos, 1982) and cortical cells (leventhal, 1983; leventhal et al., 1984) subserving peripheral vision respond best to stimuli that are oriented radially, i.e., like the spokes of a wheel with the area centralis at the hub. we have extended this work by comparing directly the distributions of orientations represented in topographically corresponding regions of retina and visual cortex. both central and peripheral regions were studied. the relations betwee ...	1986	3958796
[the systematic evaluation of medical procedures. apropos of the effectiveness of parallel medicine].		1986	3961360
[ratio of the frequency of chromosome aberrations and sister chromatid exchanges to the dose of the mutagenic exposure to cyclophosphamide in vivo and in vitro].	cyclophosphamide (cp) and its metabolites were used to compare the rate of chromosomal aberrations (ca) and sister chromatid exchange (sce) in the rabbit lymphocytes in vivo and in vitro. the dose-dependent increase of cytogenetic effects rate appeared to be of linear and exponential dependence for sce and ca, respectively, both in vivo and in vitro. the regression equation coefficients coincided in in vivo and in vitro experiments.	1986	3697494
toxicity of essential oils on toxigenic and nontoxigenic fungi.		1986	3697529
evidence for the presence of a novel biosynthetic pathway for norspermidine in vibrio.	enzymatic studies of the cell extracts of vibrio alginolyticus and v. parahaemolyticus provided evidence that there exists a novel biosynthetic pathway for norspermidine (nh2(ch2)3nh(ch2)3nh2), a major polyamine species. in this pathway, the schiff base formed between aspartic beta-semialdehyde and 1,3-diaminopropane is first reduced by a nadph-dependent enzyme to yield "carboxynorspermidine" (nh2(ch2)3nh(ch2)2ch(nh2)cooh), which is in turn decarboxylated by a pyridoxal phosphate dependent enzym ...	1986	3697846
prevention of doxorubicin myocardial toxicity in mice by reduced glutathione.	the effect of reduced glutathione (gsh) on acute myocardial toxicity due to doxorubicin (dxr) was investigated. at 20 mg/kg i.p. dxr was 100% lethal to balb/c x dba/2 f1 mice. at 500 or 1000 mg/kg i.p. gsh significantly decreased the lethality (p less than 0.01). electron micrographs of the myocardium from dxr-treated mice showed narrowing of myofibrils, edematous cytoplasm, and mitochondrial swelling which were detectable at day 2, was strongest at day 14, but had disappeared by day 56. light m ...	1986	3697994
destruction and repair of mammary gland parenchyma of cows infected with foot-and-mouth disease.	bovine mammary gland secretory epithelial cells show signs of infection as early as three days after exposure of lactating host dairy cows to pigs infected with foot-and-mouth disease. isolated foci of necrotic alveoli, as observed by scanning electron microscopy, increased in area and number with time. infection of individual milk synthesizing cells progressed to involve the entire secretory epithelium. alveolar luminal contents, in contrast to those in control preparations, consisted of heavy ...	1986	3700734
experimental plague infection in south african wild rodents.	susceptibility studies were undertaken to determine the response of some south african wild rodent species to experimental plague (yersinia pestis) infection. a degree of plague resistance was found in three gerbil species captured in the plague enzootic region of the northern cape province, these being the namaqua gerbil, desmodillus auricularis, (ld50 1 x 10(6) organisms), the bushveld gerbil, tatera leucogaster, (ld50 9.1 x 10(5)) and the highveld gerbil, t. brantsii (ld50 4 x 10(2)). animals ...	1986	3701038
[pathogenic action of the plague microbe on the flea xenopsylla cheopis and the ultrastructure of the causative agent at various times of its stay in the vector].	pathology of gastro-intestinal tract of xenopsylla cheopis fleas infected with plague microbe was determined by means of electron microscopy. ultrastructure of plague microbe during different periods of its stay in the vector was studied.	1986	3703571
in vivo transfer of antibiotic resistance to a strain of salmonella arizonae.	two trials were conducted to determine the transferability of antibiotic resistance in vivo between two strains of enteric bacteria. newly hatched turkey poults were inoculated per os with a strain of escherichia coli resistant to kanamycin, tetracycline, and ampicillin, a strain of salmonella arizonae resistant to nalidixic acid and streptomycin, or both strains. kanamycin was added to the drinking water of some poults. to test for s. arizonae cells which had received transferable resistance de ...	1986	3703777
cutaneous leishmaniasis from belize--treatment with ketoconazole.		1986	3708895
preparation of [3h]collagen for studies of the biologic fate of xenogenic collagen implants in vivo.	reduction of a commercially available, pepsin-solubilized, bovine dermal collagen (vitrogen 100) with sodium [3h]borohydride provided radiolabeled collagen preparations with specific activities ranging from 7.1-12.0 muci/mg collagen. these specific activities were 2-3 times greater than those obtained by reduction of intact rat tail tendon collagen under similar conditions. the alpha, beta, and higher aggregate components of type i collagen were radiolabeled as well as the alpha component of a s ...	1986	3711680
further investigation of the effect of chronic ethanol on the electric membrane properties of adult drg neurons in cell culture.	neural cell cultures were used to investigate the effects of acute and chronic ethanol exposure on electric membrane properties (emp) of adult mouse dorsal root ganglion (drg) neurons. these results in combination with results from additional experiments involving chronic exposure to hyposomotic medium provided further support for the hypothesis that the abnormal emp caused by chronic ethanol exposure involved neuronal membrane adaptation to an ethanol-induced membrane expansion.	1986	3714130
deformability and other rheological interactions of red blood cells in electronic cell sizing.	the red blood cell apparent-size spectrum, obtained using resistive pulse spectroscopy (rps) with typical electrical response times, is characterized by a bimodality, which in turn is quantified by a bimodality index. the magnitude of the index reflects the nonuniformity in distribution of particle trajectories within the orifice, itself a function of cell deformability. in measurements of mixed populations of glutaraldehyde-fixed and native cells, the index is found to be linearly dependent on ...	1986	3719088
morphogenesis of sclerotome and neural crest in avian embryos. in vivo and in vitro studies on the role of notochordal extracellular material.	the distribution of sclerotome and neural crest cells of avian embryos was studied by light and electron microscopy. sclerotome cells radiated from the somites towards the notochord, to occupy the perichordal space. neural crest cells, at least initially, also entered cell-free spaces. at the cranial somitic levels they moved chiefly dorsal to the somites, favouring the rostral part of each somite. these cells did not approach the perichordal space. more caudally (i.e. trunk levels), neural cres ...	1986	3719666
relating extracellular potentials and their derivatives to anisotropic propagation at a microscopic level in human cardiac muscle. evidence for electrical uncoupling of side-to-side fiber connections with increasing age.	elucidation of the mechanisms of cardiac conduction disturbances leading to reentry will require resolution of the details of multidimensional propagation at a microscopic size scale (less than 200 micron). in practice, this will necessitate the combined analysis of extracellular and transmembrane action potentials. the purpose of this paper is to demonstrate the relationships between the time derivatives of the extracellular waveforms and the underlying action potentials in the experimental ana ...	1986	3719925
a secretory phospholipase c-like enzyme in the bovine epididymal caput.	an enzyme hydrolysing the synthetic substrate p-nitrophenylphosphorylcholine was highly active in the epididymal caput and very low or absent in other parts of the bovine reproductive organs. this enzyme was also found in the secretory fluid obtained from the epididymal caput but much lower activities were encountered in cauda epididymis and seminal plasma. the enzyme displayed an optimum at ph 6.5, an mr of about 66000, a pi value of 5.0 and was suppressed by chelating agents and reactivated by ...	1986	3720958
renal function in the fetal lamb: a chronic model to study physiological effects of ureteral ligation and deligation.	between 53 and 98 days of gestation 11 fetal lambs were subjected to unilateral ureteral ligation. in 8 fetuses re-exploration was performed 35 to 41 days after ligation. chronic catheterization of the obstructed kidney, bladder and fetal vascular system was used for analysis of physiological data. average creatinine clearance was 1.35 +/- 0.067 ml. per minute in the control kidney and 0.226 +/- 0.057 ml. per minute in the obstructed kidney after diversion. free water clearance, osmolar clearanc ...	1986	3723669
isolation and characterization of apolipoprotein b-100.		1986	3724504
[experiments to overcome lactation anestrus in dairy sheep].	an experiment was carried out with three groups of ewes (f1 crosses--black face plevenska x east friesian) used for milk to overcome the lactation anestrus. use was made of megestrol acetate and pms during the mating season. the animals of the 1st (control) group were dried off one month prior to the service-period. those of the iind group were dried off at the beginning of the insemination period; and those of the iiird one were milked during the season and up to the third month of pregnancy. t ...	1986	3727389
directionality and alignment of the foveal receptors, assessed with light scattered from the human fundus in vivo.	the directionality of the foveal receptors of four subjects is assessed with a technique based on light scattering, and a psychophysical technique. the width and orientation of the absorbance diagram (difference between the scattering diagram in a bleached and unbleached state of the visual pigment) are compared with the stiles-crawford function. results show that the directionality in the scattering diagram points into approximately the same direction as the s-c function, but the width of the s ...	1986	3727414
multiple lung cavities in a 12-year-old girl with bubonic plague, sepsis, and secondary pneumonia.	after one week of nonspecific symptoms, pneumonia and an extremely tender and enlarged cervical lymph node developed in a 12-year-old girl who lived in an area of new mexico known to have plague-infected rodents. cultures from an aspirate of the node, her sputum, and blood all showed growth of yersinia pestis. she was treated successfully with aminoglycosides and tetracycline. as the pneumonia resolved, areas of consolidation were replaced by cavitary lesions.	1986	3728514
a microplate enzyme-linked immunosorbent assay for measuring antibody to anaplasma marginale in cattle serum.	an enzyme-linked immunosorbent assay (elisa) method is described for measuring antibody against anaplasma marginale in cattle serum. this method was more sensitive and objective than a previously described elisa method for a. marginale and possible reasons for this are discussed. all 83 cattle experimentally infected with a. marginale (81) or a. centrale (2) developed demonstrable specific antibody but the serums of 98.8% of 839 cattle from cattle tick-free areas did not react by elisa; 378 seru ...	1986	3729834
a drug interaction study of ceftriaxone and frusemide in healthy volunteers.	ceftriaxone, a recently developed cephalosporin significantly reduced the diuretic activity of frusemide in rats. for this reason and because an interaction of unknown mechanism is well established between frusemide and some cephalosporins, we studied the interference of ceftriaxone with the diuretic effect of frusemide in healthy volunteers. twelve subjects received frusemide (40 mg p.o.) or placebo in combination with ceftriaxone (2 g i.v.) or saline on 4 different days (cross-over, randomized ...	1986	3733274
a drug interaction study of ceftriaxone and frusemide in healthy volunteers.	ceftriaxone, a recently developed cephalosporin significantly reduced the diuretic activity of frusemide in rats. for this reason and because an interaction of unknown mechanism is well established between frusemide and some cephalosporins, we studied the interference of ceftriaxone with the diuretic effect of frusemide in healthy volunteers. twelve subjects received frusemide (40 mg p.o.) or placebo in combination with ceftriaxone (2 g i.v.) or saline on 4 different days (cross-over, randomized ...	1986	3733274
increased 5-hydroxytryptamine and norepinephrine release from rat brain slices by the red sea flatfish toxin pardaxin.	the effect of the red sea flatfish toxin pardaxin was examined on k+-evoked and on basal release of either [3h]norepinephrine or [3h]5-hydroxytryptamine from preloaded rat cortical slices. the k+-induced release of the neurotransmitters was stimulated in a dose-related manner at concentrations ranging from 0.5 to 4 micrograms/ml. basal release of the two transmitters was elevated to a lesser extent. although the stimulation of evoked release was approximately equivalent for the two neurotransmit ...	1986	3734799
neuropeptide investigations with an ovine surgical model.	an ovine surgical model for peptide investigations is presented. techniques for exteriorization of the carotid artery, catheterization of the sagittal sinus, hypophysectomy via a transnasopharyngeal approach, and ventricular and cisternal cannulation in the sheep are employed within the model. several experimental applications in neurosurgical research are presented.	1986	3736803
[bactericidal activity of immune serum against y. pestis].		1986	3742576
heterogeneity of the enzymatic apparatus of the cells of the sinusoidal system of the spleen--a histochemical study.		1986	3744174
effect of training on plasma anabolic and catabolic steroid hormones and their response during physical exercise.		1986	3744643
probing the phagolysosomal environment of human macrophages with a ca2+-responsive operon fusion in yersinia pestis.	several microorganisms, including yersinia sp., salmonella sp., brucella sp., mycobacterium sp. and leishmania sp., have successfully adapted to grow within macrophage phagolysosomes. infections caused by these intracellular pathogens are among the most difficult to treat. as part of an antimicrobial strategy directed at modifying the phagolysosomal environment to the disadvantage of these important pathogens, we are defining the ambient conditions within the organism-containing phagolysosome. t ...	1986	3748170
aldehyde-induced inhibition of dna repair and potentiation of n-nitrosocompound-induced mutagenesis in cultured human cells.		1986	3749045
myocardial mechanics in allylamine-induced myocardial fibrosis.	to examine the effect of fibrosis on myocardial mechanics, we studied isolated left ventricular papillary muscles from 18 rats given 0.1% allylamine, an agent known to cause myocardial fibrosis, in drinking water for 4-8 wk. six control rats were given tap water. left ventricular hydroxyproline concentration was higher in the allylamine-treated group [3.47 +/- 2.12 vs. 2.10 +/- 0.66 (sd) micrograms/mg dry wt; p less than 0.01]. because of variable and heterogeneous involvement of the ventricle b ...	1986	3752275
symptomatic osteoporosis in a man with hyperprolactinemic hypogonadism.	a 66-year-old man presented with height loss, back pain due to an l4 vertebral compression deformity, and marked osteopenia shown by radial photon absorptiometry. the patient had small testes and a long history of infertility and impotence. plasma testosterone was low (130 ng/dl; normal, 300 to 1000 ng/dl) with marked elevation of serum prolactin (590 ng/ml; normal, 0 to 15 ng/ml). high-resolution computed tomography showed an invasive pituitary macroadenoma with a secondary empty sella turcica. ...	1986	3752759
open lung biopsy in mycoplasma pneumoniae pneumonia.	pathologic descriptions of mycoplasma pneumoniae pneumonia in humans are scarce, and most descriptions are from autopsy studies. we studied open lung biopsy specimens from six patients with proved or probable m pneumoniae pneumonia and reviewed the findings on m pneumoniae pneumonia that have been reported in the literature, as well as the electron microscopic characteristics of the organism, to document the morphologic spectrum. five of our six cases showed a typical but nonspecific polymorphon ...	1986	3753567
is reactive gliosis a property of a distinct subpopulation of astrocytes?	we have shown previously that the a2b5 monoclonal antibody distinguishes two types of glial fibrillary acidic protein-containing astrocytes in semithin frozen sections of adult rat optic nerve: a2b5- (type-1) astrocytes are found mainly at the periphery of the nerve, where they form the glial limiting membrane, while a2b5+ (type-2) astrocytes are found mainly in the interior of the nerve and constitute more than 65% of the astrocytes in the adult optic nerve. in the present study we show that al ...	1986	3753719
the effect of arylazido aminopropionyl atp (anapp3) on inhibition of pelvic nerve evoked contractions of the cat urinary bladder.	previously reported work has provided evidence that arylazido aminopropionyl atp (anapp3), a p2-receptor antagonist, blocks the effects of atp and other purine analogs, in the urinary bladder of the cat. this antagonism appeared limited to those effects mediated by p2-receptors, such as contraction of the urinary bladder. however, it was noted that anapp3 did alter the effects of adenosine, mediated through p1-receptors, in some preliminary experiments. a series of experiments was undertaken to ...	1986	3753940
evidence of a role for calmodulin in the regulation of calcium release from skeletal muscle sarcoplasmic reticulum.	the effect of calmodulin and calmodulin inhibitors on the "ca2+ release channel" of "heavy" skeletal muscle sarcoplasmic reticulum (sr) vesicles was investigated. sr vesicles were passively loaded with 45ca2+ in the presence of calmodulin and its inhibitors, followed by measurement of 45ca2+ release rates by means of a rapid-quench-millipore filtration method. calmodulin at a concentration of 2-10 microm reduced 45ca2+ efflux rates from passively loaded vesicles by a factor of 2-3 in media conta ...	1986	3754148
the chemical and enzymatic oxidation of hematohemin ix. identification of hematobiliverdin ix alpha as the sole product of the enzymatic oxidation.	oxidative cleavage of hematohemin ix in pyridine solution in the presence of ascorbic acid (coupled oxidation), followed by esterification of the products with boron trifluoride/methanol produced the four possible hematobiliverdin dimethyl esters in 11.1% overall yield. transetherifications took place simultaneously with the esterification reaction and resulted in the formation of the dimethyl ester of hematobiliverdin ix gamma 8a,13a-dimethyl ether (1.8%), the dimethyl ester of hematobiliverdin ...	1986	3754208
complement-dependent lysis of erhlich ascites tumor cells by human serum (ascitolysin) is lowered in cancer patients and raised in pregnant women.	normal human serum has been shown to contain igm antibodies that are lytic to ehrlich ascites tumor cells in the presence of complement. we call this activity ascitolysin (al). using a 51cr release assay, the al titers of 164 normal sera, 211 sera from patients with non-malignant disease, 62 sera from 25 pregnant women and 170 sera from cancer patients were estimated. the patient sera were taken preoperatively, and were assigned to the cancer or non-cancer group after histological diagnosis. in ...	1986	3754211
nucleotide sequence of a full-length cdna for mouse cytoskeletal beta-actin mrna.		1986	3754329
stabilization of intracellular recordings in the cat spinal cord using high-frequency ventilation.	stabilization of intracellular recordings by a substantial reduction of respiratory motion may be accomplished by the use of high-frequency ventilation (600-800 breaths/min). the technique is a simple one and enables maintenance of relatively constant blood gas tensions. although the improved stability was demonstrated in experiments performed on alpha motoneurones in the thoracic spinal cord, it is anticipated that a reduced respiratory movement should benefit recordings in other spinal and sup ...	1986	3756629
yersinia enterocolitica inguinal lymphadenitis.	inguinal lymphadenitis is associated with a well-defined group of etiologic agents including many sexually transmitted diseases and nonvenereal agents including yersinia pestis (bubonic plague). we report herein the first case of a second yersinia species--yersinia enterocolitica--presenting like bubonic plague with bilateral inguinal lymphadenitis.	1986	3757476
acute desensitization to angiotensin ii: evidence for a requirement of agonist-induced diacylglycerol production during tonic contraction of rat aorta.	a possible role for protein kinase c during the tonic phase of arterial contraction was examined in rat aorta by observing the effects of the phorbol ester, 12-o-tetradecanoylphorbol-13-acetate (tpa), on angiotensin ii (aii)-induced responses. the ability of aii and phenylephrine (pe) to induce diacylglycerol (dag) production was monitored as agonist-stimulated 32p-labelling of phosphatidic acid (pa). aii (5 x 10(-7) m) causes only a transient contractile response, while pe (10(-5) m) causes a s ...	1986	3758157
prevalence of toxoplasma antibodies in southern louisiana swine.	sera from 1,219 pigs slaughtered in acadian louisiana in 1980 and 1981 were tested using the indirect hemagglutination test and microtiter system for toxoplasma antibodies and 19.2% had titers of 1:64 or higher. pigs slaughtered in the colder months, november to march, had significantly higher seroprevalences than pigs slaughtered in the warmer months. 24.6% of 236 farm bled sows and gilts had similarly high titers.	1986	3759353
progesterone receptor synthesis and degradation in mcf-7 human breast cancer cells as studied by dense amino acid incorporation. evidence for a non-hormone binding receptor precursor.	we have used the technique of density labeling of proteins by biosynthetic incorporation of 2h, 13c, 15n (dense) amino acids to study the synthesis and degradation rates of the progesterone receptor in mcf-7 human breast cancer cells. in cells grown in the absence of progestin, sucrose gradient shift analyses reveal that it takes 17 h for the normal density progesterone receptor levels to be reduced to half the initial value, whereas in the presence of 10 nm of the synthetic progestin [3h]r5020, ...	1986	3759961
the organization of the reciprocal connections between the subiculum and the entorhinal cortex in the cat: i. a neuroanatomical tracing study.	the connections between the subiculum (sub) and the entorhinal cortex (ec) were studied in the cat with retrograde and anterograde tracing techniques. injections of the retrogradely transported tracer wga-hrp at different levels along the septotemporal axis of the subiculum result in labeled neurons predominantly in the medial entorhinal cortex (mea) in the superficial layers ii and iii. in the deep layers labeled cells are found more widespread over the ec. the superficially located labeled ec ...	1986	3760250
cardiopulmonary hypoxic response 5 years postpneumonectomy in beagles.	ventilatory hypoxia was used to study the effects on pneumonectomy on the right heart and pulmonary vascular bed in purebred beagle dogs. operated animals underwent pneumonectomy at 6 to 10 weeks of age (six, group i) or at 1 year (eight, group ii). eight unoperated adult beagles served as controls (group iii). five years following pneumonectomy, exposure to 10% o2 ventilation indicated that group i animals were more reactive to hypoxia than either group ii or iii. increases in pulmonary flow we ...	1986	3762129
high chronic ambient temperature stress effects on broiler acid-base balance and their response to supplemental ammonium chloride, potassium chloride, and potassium carbonate.	four experiments were conducted to evaluate the effect of heat stress on acid-base status and the birds' response to supplemental kcl and kco3. the corn-soybean meal fish-meal basal ration (.73% k+) contained more k+ and cl- than is recommended by the national academy of sciences for chicks reared under near optimal conditions (24 c and 55% relative humidity). chicks reared under continuous thermostress (35 c, 70% relative humidity) exhibited panting phase blood alkalosis (ph of 7.46). supplemen ...	1986	3774744
endosulfan induced inhibition of 3h-5-hydroxytryptamine uptake in platelets.	endosulfan, a chlorinated pesticide, is widely used to control various insect pests. rats exposed to 1 mg and 3 mg endosulfan/kg for periods of 10, 30, and 60 days showed significant (p less than 0.05) inhibition of [3h]5-hydroxytryptamine (5-ht) uptake by platelet-rich plasma (prp) in an ex vivo study. rats treated with endosulfan (1 and 3 mg/kg) up to 60 days elucidated a marked inhibition of adp-induced aggregatory responses of the platelets. incubation of prp with 10 microm and 100 microm en ...	1986	3775804
role of glucagon in splanchnic hyperemia of chronic portal hypertension.	the role of glucagon as a blood-borne mediator of the hyperdynamic circulation associated with chronic portal venous hypertension was assessed in the rat portal vein stenosis model. selective removal of pancreatic glucagon from the circulation was achieved by intravenous infusion of a highly specific glucagon antiserum. blood flow to splanchnic organs, kidneys, and testicles was measured with radioactive microspheres, and the reference-sample method. glucagon antiserum had no effect on blood flo ...	1986	3777172
effects of sex steroids on the development of two granule cell subpopulations in the rat accessory olfactory bulb.	the effects of postnatal male castration on day 1 (d1) after birth and female androgenization on accessory olfactory bulb (aob) light and dark granule cell populations were studied. control males showed a greater number of both light and dark aob granule cells than females. postnatal treatment reversed these differences in the light granule cells. female androgenization on d1 does not affect the number of aob dark granule cells. however, male orchidectomy also on d1 significantly decreases the n ...	1986	3779445
[effect of plasmid r245 on induced mutagenesis in yersinia pestis ev76].		1986	3785255
the nucleotide sequence of the 5 s rrna of rhodobacter capsulatus atcc 23782.		1986	3786137
nucleotide sequence of the gene encoding the matrix protein of newcastle disease virus.	the nucleotide sequence of the gene encoding the matrix (m) protein of the beaudette c strain of newcastle disease virus (ndv) has been determined from overlapping cdna clones. control sequences typical of paramyxovirus mrna start and polyadenylation signals have been identified. assuming that the m gene starts and finishes at these sequences, the m gene is 1241 nucleotides long and encodes one long open reading frame of 364 amino acids, corresponding to a polypeptide of molecular weight 39605, ...	1986	3786143
nucleotide sequence of the gene encoding the matrix protein of newcastle disease virus.	the nucleotide sequence of the gene encoding the matrix (m) protein of the beaudette c strain of newcastle disease virus (ndv) has been determined from overlapping cdna clones. control sequences typical of paramyxovirus mrna start and polyadenylation signals have been identified. assuming that the m gene starts and finishes at these sequences, the m gene is 1241 nucleotides long and encodes one long open reading frame of 364 amino acids, corresponding to a polypeptide of molecular weight 39605, ...	1986	3786143
coagulant versus amidolytic properties of human and bovine thrombins: implications in standardization and diagnostic usage.	since the introduction of synthetic peptide substrates for thrombin, many amidolytic methods for the determination of at iii, heparin cofactor ii, prothrombin, thrombin, platelet factor 4, and absolute levels of heparin have been proposed. all of these methods utilize thrombin that has been standardized in coagulant assays using either fibrinogen (human or bovine) or citrated plasma substrates. these thrombin preparations may contain noncoagulant forms of thrombin, prothrombin fragments, and oth ...	1986	3787268
failure of norfloxacin to eradicate chlamydia trachomatis in nongonococcal urethritis.	norfloxacin has some activity in vitro against chlamydia trachomatis and ureaplasma urealyticum, although not at levels attainable in serum. in this study, norfloxacin was administered (400 mg orally twice daily for 10 days) to men with acute nongonococcal urethritis. of 25 men from whom c. trachomatis was initially isolated, 21 had the organism reisolated at the first follow-up visit posttreatment, and there were minimal changes in the number of inclusion-forming units in culture. ultimately, a ...	1986	3789692
[restoration of immunologic responsiveness by psk in tumor-bearing animals].	psk is a protein-bound polysaccharide prepared from cultured mycelium of the basidiomycete coriolus versicolor. effects of psk on the immunologic responsiveness in tumor-bearing animals were investigated using syngeneic or allogeneic tumors in mice (lewis lung carcinoma, b16 melanoma, meth a fibrosarcoma, adenocarcinoma 755, x5563 plasmacytoma, colon 26, mopc 31c myeloma, sarcoma 180 and ehrlich carcinoma), rats (bc47 bladder carcinoma, walker 256 sarcoma and ah7974 hepatoma), hamsters (ha-1t tu ...	1986	3789758
scleredema in an infant.	a 3-month-old infant with scleredema had associated cytomegalovirus pneumonia with a rapidly fatal course. because differentiation of scleredema from scleredema neonatorum, subcutaneous fat necrosis, and scleroderma may be difficult, histologic diagnosis is important. cetylpyridinium chloride was used successfully as a fixative to demonstrate acid mucopolysaccharides histologically.	1986	3789776
comparison of histones in normal and xeroderma pigmentosum lymphoblastoid cells.	histones from normal human and xeroderma pigmentosum, complementation group a (xpa), lymphoblastoid cells were compared both quantitatively, qualitatively and for binding affinity for dna. electrophoretic examination of the histones showed that all five major histone species were present in both cell groups and that there were no quantitative differences between normal and xpa histones. binding affinity to [3h] mammalian dna of the histones was determined. no significant differences were observe ...	1986	3791423
factors affecting prerace dehydration and performance of racing greyhounds.	several factors, including age, sex, race number, post position, and race class, were evaluated for relationships to prerace percentage body weight loss and performance in all greyhounds racing during august 1983 in oregon. the incidence of percentage body weight loss greater than or equal to 2.5% was low (5%). race number had a direct effect on weight loss; the later the dogs raced, the more weight loss they experienced. female dogs were 3 times more likely to lose greater than 2.4% of body wei ...	1986	3793595
potassium uptake in the mouse submandibular gland is dependent on chloride and sodium and abolished by piretanide.	nervous or hormonal stimulation of salivary secretion in vivo is associated with a pronounced efflux of k+ from the secretory, acinar cells into the blood. this k+ efflux is followed in the post-stimulus period by a reuptake of k+ into the glandular tissue. in the present study we monitor the changes in [k+] of physiological solutions perfusing a flow chamber containing isolated segments of mouse submandibular glands. nervous stimulation or the application of exogenous acetylcholine (ach, 10(-5) ...	1986	3795114
[a subacute oral toxicity study of t-2588 in juvenile rats].	a subacute toxicity study on t-2588 was carried out in juvenile rats (6 days-old 48 males and 48 females) by oral administrations at dose levels of 250, 500 and 1,000 mg/kg/day for 1 month. the results obtained were summarized below. during the study, there were no clinical signs considered to be related to the treatment. there were no t-2588-related abnormalities in urinalysis, hematological examinations, biochemical examinations and ophthalmological examinations. slight increases in relative l ...	1986	3795478
pathology of the vertebral column of horses with cervical static stenosis.	specimens of ligamentum flavum, joint capsule, and dorsal lamina were collected at surgery or necropsy from 25 horses with cervical static stenosis. all horses had myelographic evidence of dorsal compression of the spinal cord caused by soft tissue and/or bone in the caudal cervical area, primarily at c6-7. most horses also had radiographic evidence of degenerative joint disease of articular facets. histologically 19 horses had osteosclerosis and cartilage retention in the dorsal lamina, and 24 ...	1986	3750733
[virulence, viability and antibiotic sensitivity of the causative agent of plague growing on nutrient media at 28 and 37 degrees c].	a comparative study of virulence, viability and antibiotic sensitivity of y. pestis strains grown at 28 degrees c and 37 degrees c in yeast-casein medium, yeast medium with hottinger's meat digest and yeast medium with protein hydrolysate obtained from sunflower seed groats has been made. these media have been found to be suitable for the prolonged cultivation of y. pestis at 28 degrees c and 37 degrees c, for the determination of its sensitivity to antibiotics, as well as for the preservation o ...	1986	3727879
[incidence of ureaplasma urealyticum and mycoplasma hominis in persons examined for sterility].		1986	3719732
plasmid-like properties of the four virulence-associated factors of yersinia pestis.	the virulence-associated factors of yersinia pestis, which determine the abilities to produce pesticin i (pst+), capsular fraction i antigen (fra+), v and w antigen complex (vwa+) and a cell-surface component for adsorption of exogenous pigments (pgm+), were independently eliminated by cultivation of the cells in the presence of acridine orange, ethidium bromide or sodium dodecyl sulfate at a subinhibitory concentration. a virulent y. pestis strain, yreka, harbored at least five extrachromosomal ...	1986	3796319
[mobilization of plasmid determinants of yersinia pestis using the plasmid pka7].	yersinia pestis plasmids having inserted tn-movable elements and determining pesticinogenicity, ca2+-dependence, production of mice toxin and fraction i have been mobilized for conjugal transfer into y. pestis, y. pseudotuberculosis or e. coli cells by plasmid pka7. plasmid determinants have been transferred with the frequencies 10-4-10-7 per donor cell. the plasmid dna of high molecular mass has been identified in transconjugant cells. plasmid mobilization in yersinia pestis is supposed to be d ...	1986	3796653
the structure of the cuticle and sheath of the infective juvenile of trichostrongylus colubriformis.	the infective third-stage juvenile of trichostrongylus colubriformis is surrounded by its own cuticle as well as the incompletely moulted cuticle of the second-stage juvenile, which is referred to as the sheath. the sheath comprises an outer epicuticle, an amorphous cortical zone, a fibrous basal zone and an inner electron-dense layer. the basal zone of the sheath consists of three layers of fibres; the fibres are parallel within each layer, but the fibre direction of the middle layer is at an a ...	1986	3799009
[changes in the characteristics of glucocorticoid hormone binding in human peripheral blood lymphocytes during myocardial infarct].	competitive binding was used to determine the number of binding sites to glucocorticoid hormones in peripheral blood leukocytes of normal subjects, patients with unstable angina pectoris and patients with myocardial infarction. a sharp (2-3-fold) increase in the number of binding sites was observed in patients with myocardial infarction during the first 24 hours after the onset of the disease.	1986	3801148
reduced number of [3h]nicotine and [3h]acetylcholine binding sites in the frontal cortex of alzheimer brains.	nicotinic cholinergic receptors were measured in human frontal cortex using [3h]nicotine and [3h]acetylcholine (in the presence of atropine) as receptor ligands. a parallel marked reduction in number of [3h]nicotine (52%; p less than 0.01) and [3h]acetylcholine (-55%; p less than 0.05) binding was found in the frontal cortex of alzheimer brains (ad/sdat) when compared to age-matched control brains. as a comparison the number of muscarinic receptors was quantified using [3h]quinuclidinyl benzilat ...	1986	3808458
[bacillus gordonae sp. nov., a new species belonging to the second morphological group, degrading various aromatic compounds].	thirty strains were isolated from pasteurized soil samples by enrichment culture in aerobiosis at 32 degrees c in a minimal medium containing one of the following compounds as sole source of carbon and energy: quinate, p-hydroxybenzoate, phthalate, isophthalate or trimellitate. these bacteria were rods (0.8 x 2-7 micron), motile by peritrichous flagella. endospores were oval (1.4-1.8 x 2 micron) and distinctly swelled the sporangia. the gram reaction was variable but the gram type was positive. ...	1986	3674781
dynamic model of the equine hindlimb during the swing phase.	a dynamic model is developed to describe the swing phase of the hindlimb of a normally walking horse. the limb was represented by four rigid segments constrained to move in a sagittal plane only. the mathematical equations of motion of this four-element pendulum were formulated using lagrange's theorem. the morphometric parameters from the hindlimb segments of 3 horses were determined using high-speed film analysis. five muscle groups were incorporated in the model. muscle activity was derived f ...	1986	3811820
human term placenta releases placental protein 10 (pp10) in tissue culture.	placenta, decidua, chorion and amnion were studied to determine the tissue concentration of placental protein 10 (pp10). as measured by radio-immunoassay, all tissues studied were found to contain approximately the same concentration of pp10, in both early and late pregnancy. the release of pp10 was studied in tissue culture, first by using explants of placenta, decidua, amnion and chorion in four preliminary studies. only the placenta released significant amounts of pp10 into the culture medium ...	1986	3811843
antibacterial activity and ototoxicity in guinea pigs, and nephrotoxicity in rats of arbekacin.	arbekacin (hbk), 1-n[(s)-4-amino-2-hydroxybutyl]-3',4'-dideoxykanamycin b, showed broad antibacterial spectra against gram-positive and gram-negative bacteria including pseudomonas aeruginosa. it was also effective against gentamycin- or tobramycin-resistant bacteria. hbk was resistant to various aminoglycoside-inactivating enzymes except for aac (2') and aac (6')-iv, both of which slowly inactivated it. even at higher dosages (150 mg/kg i.m. or greater, which resulted in some deaths), hbk never ...	1986	3814212
lysosomal membrane stabilization by enfenamic acid.		1986	3583334
development of highly immunogenic variants of a rat fibrosarcoma line during in vitro cultivation.	rat fibrosarcoma kmt-17 cells decreased in tumorigenicity when cultured in vitro. eight clones derived from cultured kmt-17 cell lines (c-kmt-17) were examined for their tumorigenicity, immunosensitivity, and immunogenicity. all the clones were less or nontumorigenic in normal syngeneic rats than kmt-17 cells maintained in vivo. all eight clones produced tumors in rats immunosuppressed with 600 rad 60co; differences in degree of tumorigenicity were seen among clones in rats irradiated with 250 r ...	1986	3632916
comparative evaluation of identification systems for testing methicillin-resistant strains of staphylococcus aureus.	several commercial systems are available to distinguish between staphylococcus aureus and the coagulase-negative species of the micrococcaceae family. four latex agglutination systems (accu-staph, serostat, staphaurex, and staphylatex) and two hemagglutination systems (hemastaph and staphyloslide) were compared for their performance in the rapid identification of 232 isolates of staphylococci, including 114 of methicillin-resistant s. aureus. accu-staph, staphaurex, and staphyloslide correctly i ...	1986	3638308
[comparative study of the activity of arginase isoenzymes in brain tumors of humans and experimental animals].	isoenzyme spectrum and total activity of arginase were studied in rat brain during growth of transplanted neurinoma and glioma as well as in malignant tissues of human brain. after transplantation of the tumors two peaks of arginase activation were observed within 2-4 days in rat brain tissues and within 16 days in tumoral tissue. positively charged isoenzyme i of arginase was mainly activated but activity of neutral isoenzyme ii was unaltered or slightly decreased. in human brain tumors activit ...	1986	3811280
[escherichia coli neuraminidase].	the intracellular form of neuraminidase has been detected in e. coli and proteus vulgaris. neuraminidase has been isolated from e. coli hb 101 cells and purified 118-fold. some physico-chemical properties of this enzyme have been studied.	1986	2422854
the response of the lung to foreign compounds that produce free radicals.		1986	2423026
characterization of receptors regulating secretory function in the fundic mucosa.	a model for a present view of the major pathways and receptors mediating function in the canine fundic mucosa is depicted in figure 1. gastrin has direct actions on the parietal cell and on the somatostatin cell; action on the parietal cell, but not somatostatin cell, is potentiated by histamine. in contrast, gastrin action on the somatostatin cell is potentiated by beta-adrenergic agonists. the potency of h2 blockers against gastrin may reflect blockage by these inhibitors of the stimulatory (p ...	1986	2423027
separation and characterization of four different amylases of entamoeba histolytica. ii. characterization of amylases.	purified e. histolytica amylases iii to vi were characterized by their hydrolytic behaviour towards 4-nitrophenyl alpha-malto-oligosaccharides, malto-oligosaccharides, amylose, amylopectin, glycogen and y-cyclodextrin. the influence of specific inhibitors on the amylase activity of e. histolytica was examined and compared with typical alpha- and beta-amylases. amylases iii and iv showed alpha-glucosidase and glucosyltransferase activity by cleaving terminal non-reducing glucose from pnpg1 (iii, ...	1986	2423098
the wellcome trust lecture. genes for antigens of plasmodium falciparum.		1986	2423947
estimation of gene expression in heterocysts of anabaena variabilis by using dna-rna hybridization.	in the filamentous cyanobacterium anabaena variabilis, specialized cells called heterocysts occur in a regular pattern along the filament and are the sites of nitrogen fixation. we used two different types of dna-excess rna hybridization techniques to estimate the number of genes expressed in recently differentiated, mature heterocysts. in the first, rna and dna were incubated in a phosphate buffer at 60 degrees c, and the hybrids were separated from the unhybridized material by hydroxylapatite ...	1986	2427500
aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia.	we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ...	1986	2430056
aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia.	we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ...	1986	2430056