Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [new directions in the study of immunity in dysentery]. | 1983 | 6346759 | |
| local iga-memory response to bacterial antigens. | 1983 | 6347004 | |
| fulminating encephalopathy and shigella flexneri infection. | 1983 | 6347094 | |
| gain of ornithine decarboxylase activity by a strain of shigella flexneri serotype 6 on the introduction of an r plasmid. | the introduction of r plasmid s-a into a strain of shigella flexneri serotype 6 resulted in the strain gaining the ability to decarboxylate ornithine. the possible reasons for the phenomenon are discussed and the result is compared with that obtained previously when the introduction of r plasmids into a different strain of the same species resulted in enhanced gas production from a range of carbohydrates. | 1983 | 6348292 |
| [joint use of antibiotics and antibiotic-resistant bifidobacteria in treating acute intestinal infections]. | acute dysentery and other acute intestinal diseases with obscure etiology are accompanied by pronounced intestinal dysbacteriosis which is manifested, on one hand, by the increased levels of escherichia, enterobacteria, staphylococci and by the appearance of klebsiella and proteus in large amounts and, on the other hand, by an essential decrease in the number of lactic acid streptococci, lactobacteria, bifidobacteria. the treatment of dysentery and other intestinal diseases with obscure etiology ... | 1983 | 6349186 |
| [causes of sequential changes in the etiological structure of dysentery and the other epidemiologic patterns of this infection]. | 1983 | 6349187 | |
| synthesis of plasmid-coded heat-labile enterotoxin in wild-type and hypertoxinogenic strains of escherichia coli and in other genera of enterobacteriaceae. | the effect of host determinants on expression of plasmid-coded heat-labile enterotoxin (lt) was examined. a collection of lt plasmids was introduced into isogenic strains of escherichia coli k-12 strains containing the wild type or hypertoxinogenic (htx-2) allele. for each plasmid tested, production of lt increased by approximately 1.5- to 3-fold in the host containing htx-2, indicating that the htx-2 allele affects a regulatory function for lt production that is common to many different enterot ... | 1983 | 6350177 |
| the influence of bacterial exotoxins and endotoxins on the phagocytic activity of human macrophages in culture. | the effect of bacterial exotoxins and endotoxins on phagocytosis was tested on human macrophages in monolayer cultures by determining the rate of zymosan particle ingestion at different toxin concentrations and incubation times. the exotoxins tested were staphylococcal alpha-toxin and diphtheria-toxin. the endotoxins used were lipopolysaccharides from salmonella typhi, salmonella typhimurium, shigella flexneri and serratia marcescens. phagocytosis was significantly impaired after prolonged incub ... | 1983 | 6350191 |
| [importation of shigella and salmonella into east germany]. | the international tourist traffic and the import of provisions also involves the importation of shigella and salmonella into the gdr. in 1980 9% of the shigella sonnei infections and 50% of the shigella flexneri infections were imported from 22 or 26 countries, in most cases from tourist countries, typical for the gdr. the salmonella are imported mainly by feed-stuff. but also the spreading particularly of polyresistant salmonella by epidemic processes independent of provisions, above all among ... | 1983 | 6351449 |
| [liquid-phase electrophoresis in the analysis of antigen-antibody interactions in erythrocyte diagnostic reagent kits]. | 1983 | 6351504 | |
| flies as a source of enteric pathogens in a rural village in thailand. | the village of ban pong in northeastern thailand was studied from january through december 1981 to determine the importance of flies as a source of enteric pathogens. the number of flies (predominantly musca domestica) increased in kitchens and animal pens in the hot dry spring, when the incidence of diarrhea was highest in the village. enterotoxigenic escherichia coli, shigella spp., non-o1 vibrio cholerae, and vibrio fluvalis were isolated from fly pools in yards (69%), animal pens (38%), bath ... | 1983 | 6351748 |
| effect of parenteral immunization on the local immunoglobulin a response of the intestine to shigella flexneri antigens. | most traditional methods of immunization involve parenteral vaccines. using a chronically isolated ileal loop model as the probe, we examined the effect of a primary parenteral immunization on the local immune response of the intestine. secretions from isolated ileal loops of rabbits given a primary parenteral immunization with shigella flexneri without adjuvant showed a small, but definite, specific immunoglobulin a (iga) response locally, whereas a vigorous serum antigen-specific igg response ... | 1983 | 6352492 |
| bacterial pathogens from the intestinal tracts of various species of cockroaches. | 1983 | 6353184 | |
| fulminant hepatic failure and shigella bacteremia. | 1983 | 6353185 | |
| [autonomic nervous system disorders in patients with sonne and flexner's dysentery]. | 1983 | 6353759 | |
| [current state of dysentery epidemiology (review of the literature)]. | 1983 | 6353765 | |
| [synthesis of beta-l-rhamnoside linked oligosaccharides of lipopolysaccharides from shigella flexneri serotype 6]. | the synthesis of the trisaccharide o-beta-l-rhamnopyranosyl-(1 leads to 4)-o-beta-l-rhamnopyranosyl-(1 leads to 2)-l-rhamnopyranose (14) and the tetrasaccharide o-2-acetamido-2-deoxy-beta-d-galactopyranosyl)-(1 leads to 2)-o-[beta-l-rhamnopyranosyl-(1 leads to 4)]-o-beta-l-rhamnopyranosyl-(1 leads to 2)-l-rhamnopyranose (21) is described. the latter structure has been proposed as the repeating unit of the o-specific side-chain of the lipopolysaccharide obtained from shigella flexneri serotype 6. ... | 1983 | 6354443 |
| sexually transmitted enteritis. | 1983 | 6355677 | |
| [biochemical aspects of the pathogenicity of shigella. protein components of aqueous extracts of virulent and non-virulent strains of shigella flexneri]. | 1983 | 6355709 | |
| invasive enteric pathogens. | invasive enteric pathogens of the salmonella or shigella genera initiate infections by invading the intestinal epithelium. depending on the species, salmonellae either translocate across the mucosa of the small intestine and cause a systemic febrile disease or they evoke a localized inflammatory response in discreet areas of the infected mucosa. the latter type of infection is characterized by gastroenteritis, and a choleragen-like enterotoxin may contribute to the symptomology. shigellae can al ... | 1983 | 6356290 |
| [trends in the spread of boyd 4 dysentery and the characteristics of its clinical picture and of the biology of its causative agent]. | the data on the proportion of s. boydii 4 in the general structure of the causative agents of dysentery, as well as in the intraspecific structure of s. boydii, in some areas of the ussr in 1977-1981 are presented. s. boydii strains 4, circulating in one of the areas where their proportion considerably increased in 1980-1981, corresponded to their taxonomic position in their biological properties, while forming a single biochemical variant according to the character of the fermentation of sorbit ... | 1983 | 6356722 |
| [dynamics of enterobacterial multiplication in a mouse model of intraperitoneal infection in the presence of iron cations as dependent on the availability and type of k antigens]. | in this work the results of research on the influence of iron cations on the characteristics of the infectious process caused by different enterobacteria (shigella sonnei, shigella flexneri, escherichia coli) are analyzed. in the experimental intraperitoneal infection of mice in the presence of saccharose and iron cations s. sonnei in phase i showed the decrease of 1g ld50 by 3-4 orders, while s. flexneri in the s-form, by not more than 1 order. the absence of correlation between the virulence o ... | 1983 | 6356728 |
| effect of human leukocyte interferon on invasiveness of salmonella species in hep-2 cell cultures. | the effect of human leukocyte interferon on the invasiveness of salmonella and shigella species in hep-2 cell cultures was examined. the intracellular and extracellular bacteria were identified by a combination of nomarski differential interference contrast microscopy and uv incident light microscopy applied on the same microscope. pretreatment of hep-2 cells with human leukocyte interferon reduced the number of salmonella typhimurium and salmonella paratyphi-b bacteria per cell and the proporti ... | 1983 | 6358041 |
| [differentiation of shigella flexneri serotype 6 from serotypes 1-5 by electrophoresis and isoelectric focusing of their elastases]. | among shigella flexneri serotypes, serotype 6 (28 strains) was individualized from serotypes 1 to 5 (43 strains) by electrophoresis and isoelectric focusing of esterases. the taxonomic status of s. flexneri serotype 6 should be reconsidered in light of this work. | 1983 | 6360018 |
| genetic transfer of a mucosal adherence factor (r1) from an enteropathogenic escherichia coli strain into a shigella flexneri strain and the phenotypic suppression of this adherence factor. | escherichia coli strain rdec-1 avidly adheres to rabbit ileal brush borders. two separate experiments were designed to determine whether pili promote this adherence. (1) adherence of strain rdec-1 was phenotypically suppressed by changing the culture medium. loss of adherence was correlated with the absence of pili. thus, growth of strain rdec-1 in penassay broth (difco laboratories, detroit, mich.) promoted both adherence and expression of pili on greater than or equal to 90% of organisms, wher ... | 1983 | 6132950 |
| antigenic relationship among virulent enteroinvasive escherichia coli, shigella flexneri, and shigella sonnei detected by elisa. | 1983 | 6134933 | |
| immunologic observations in macaques with shigella-associated periodontal disease. | 1983 | 6135769 | |
| changes in phage-sensitivity of shigella flexneri strains. ii. the f-like character of ms2-sensitive variants. | variants sensitive to male specific phage ms2 appeared among ms2 non-sensitive shigella flexneri cultures in the course of liquid medium passages. sensitivity to ms2 was lost on acridine orange treatment and was transferable into ms2 non-sensitive variants. the ms2 sensitive variants had fimbrial antigen. electron microscopy showed that ms2 phages were adsorbed on the f-like fimbriae of these variants. it was assumed that f-like plasmids determining f-like fimbrial antigen were carried by these ... | 1983 | 6140808 |
| hela cell invasiveness and o antigen of shigella flexneri as separate and prerequisite attributes of virulence to evoke keratoconjunctivitis in guinea pigs. | many rough mutants selected from isogenic smooth virulent and avirulent strains of shigella flexneri were examined for virulence, using tissue culture infection and sereny tests. many of the rough mutants isolated from a virulent smooth strain were capable of penetrating tissue culture cells but incapable of producing a positive sereny test. in contrast, we could not obtain from smooth avirulent strains any rough mutants capable of penetrating hela cells. chemical analysis of lipopolysaccharide ... | 1983 | 6187681 |
| [stimulation of replication and increase in virulence of shigella growing in rna-containing media]. | 1983 | 6188298 | |
| [causes of the nonspecific reactions with antigenic erythrocyte diagnostic reagents]. | the causes leading to the appearance of positive reactions in diagnostic titers (1:200 and higher), registered in the sera of healthy persons in tests carried out with the use of experimental and commercial lots of shigella sonnei and shigella flexneri antigenic diagnostic reagents, are analyzed. the study has shown that the appearance of nonspecific reactions may be due to the influence of the initial state of the microbial culture (the biomass used as raw material for the production of sensiti ... | 1983 | 6191477 |
| [a new antigenic variety of shigella flexneri]. | 1983 | 6194615 | |
| [use of the coagglutination reaction for identifying shigella flexneri]. | the data on the use of the coagglutination test for the identification of sh. flexneri are presented. the influence of different methods for the treatment of antisera on their heterologous reactions was studied and the advantage of coagglutinating reagents was revealed. 9 variants of coagglutinating preparations were obtained; their use made it possible to reduce the consumption of antisera and in some cases avoid their absorption. the problem of sh. flexneri classification is discussed. | 1983 | 6194634 |
| [quantitative determination of the soluble antigens of intestinal bacteria using a method of immunoenzyme analysis. i. processing of the parameters of the method]. | the parameters of the assay based on the quantitative evaluation of the neutralization of specific antibodies by the antigen under study and the subsequent detection of free antibodies on the fixed reference antigen with the aim of the quantitative determination of the specific o-antigens of salmonella, groups d, b, c1, as well as those of shigella sonnei and shigella flexneri, have been developed. the proposed method makes it possible to detect the o-antigen of the causative agent at concentrat ... | 1983 | 6195847 |
| monoclonal antibodies specific for o-antigenic polysaccharides of shigella flexneri: clones binding to ii, ii:3,4, and 7,8 epitopes. | hybrid cells producing monoclonal antibodies against the o-antigens of shigella flexneri were obtained by polyethylene glycol-mediated fusion of myeloma cells and lymphocytes from balb/c mice immunized with whole heat-killed s. flexneri bacteria of serotypes 2a and 2b. clones were selected for their binding specificity to structurally defined s. flexneri lipopolysaccharides (lps). the following three groups were identified as recognizing three different epitopes: monoclonal antibodies binding to ... | 1983 | 6196376 |
| the dental assistant: guest editorial. | 1983 | 264854 | |
| early clinical recognition of disseminated candidiasis by muscle and skin biopsy. | disseminated candidiasis was clinically diagnosed by muscle and skin biopsies in two patients with hematologic malignancies. one patient with acute promyelocytic leukemia presented with skin lesions as the sole manifestation of disseminated candidiasis. the other patient had leukemic reticuloendotheliosis and developed fever, severe myalgias, and maculopapular rash while receiving corticosteroid therapy; this patient is the first to have antemortem documentation of candida myositis as the initia ... | 1983 | 272864 |
| an improved diagnostic point to aid in radiographic interpretation. | 1983 | 264543 | |
| quantitative somatosensory thermotest. a key method for functional evaluation of small calibre afferent channels. | the quantitative somatosensory thermotest (qst) assesses the function of afferent channels concerned with sensory submodalities served by small calibre fibres. measured ramps of ascending or descending temperature are applied to the skin through a peltier contact thermode, and detection thresholds are recorded as the subject signals the onset of a particular sensation. the present study describes underlying principles, methodological aspects and normal reference values for the qst. in patients, ... | 1983 | 1628207 |
| use of igm enriched intravenous immunoglobulin (pentaglobin) in bone marrow transplantation. | in a study of 63 allogeneic and autologous bone marrow transplants, patients were randomized to receive the igm and iga enriched intravenous immunoglobulin (ivig) preparation (pentaglobin). pentaglobin has been postulated to have anti-endotoxin properties and one of the aims of the study was to measure endotoxin levels in these patients together with the clinical sequelae of infection. the anti-endotoxin effects of pentaglobin were found to reside in the igm fraction. those patients who received ... | 1983 | 1628130 |
| health manpower in new zealand. the swinging pendulum. | 1983 | 272578 | |
| family medicine residency program in semirural setting. development of a plan. | 1983 | 264607 | |
| the management of ruptured intracranial aneurysms. | 1984 | 263409 | |
| [nursing audit]. | 1984 | 261416 | |
| factors influencing small bowel changes in dermatitis herpetiformis. | factors influencing small bowel morphology in dermatitis herpetiformis (dh) were investigated by comparing patients with dh and normal small bowel biopsies to patients with dh and abnormal small bowel biopsies. the mean age of 18 patients with morphological changes in small bowel (38 years) was significantly lower (p less than 0-001) than the mean age of nine patients with normal bowel mucosa (60 years). hla typing confirmed the high frequency of hla-b8 in dh (64%) but hla-b8 was unrelated to th ... | 1984 | 270984 |
| autosomal dominant fanconi syndrome with early renal failure. | the "idiopathic" fanconi syndrome occurs mostly sporadically, occasionally as an autosomal recessive trait. however, few instances of autosomal dominant inheritance have been reported. we described a father and son with the fanconi syndrome, ie, with renal glycosuria, generalized aminoaciduria, phosphaturia, metabolic acidosis, and bone disease. no other causes of the fanconi syndrome were found. both father and son developed end stage renal disease. aminoaciduria in excess of that seen in renal ... | 1984 | 263440 |
| women hold the key to their liberation. | 1984 | 261556 | |
| [comparison of 2 methods of evaluation of skeletal maturation. 2. analysis of the correlation with another biological variable]. | 1984 | 214694 | |
| [life's holiness contra individual autonomy. medical ethics concerning blood transfusions to members of the jehovah's witnesses]. | 1984 | 425188 | |
| coherent anti-stokes raman scattering (cars) spectra, with resonance enhancement, of cytochrome c and vitamin b12 in dilute aqueous solution. | coherent anti-stokes raman scattering (cars) spectra have been obtained for ferrocytochrome c and cyano cobalamin in aqueous solution at millimolar concentrations, using a pair of tunable dye lasers pumped by a pulsed nitrogen laser. resonance enhancement was obtained by tuning the omega1 laser to the visible absorption bands of the samples. the spectral features correspond to those observed in the conventional resonance raman spectra. it appears that cars spectroscopy, with its advantageous flu ... | 1984 | 185608 |
| [advances in fibrinolysis mediated by vascular endothelial cells]. | 1984 | 2129251 | |
| surgical correction of the long face syndrome. | the combined efforts of different specialists are needed for the successful treatment of patients with the long face syndrome. both surgeons and orthodontists who recognize their own capabilities and limitations must combine their skills to achieve the best possible occlusion and facial esthetics. the surgical and orthodontic plan of therapy is designed to correct the patient's dentofacial deformity. surgical reduction of facial height and proper alignment of the teeth by orthodontic means are c ... | 1984 | 264364 |
| the shigella flexneri o-antigenic polysaccharide chain. nature of the biological repeating unit. | the sequence of monosaccharides in the biological repeating tetrasaccharide unit of shigella flexneri variant y o-antigenic polysaccharide chain was determined by subjecting three oligosaccharides of the polysaccharide, obtained by phage-sf6-mediated enzymatic hydrolysis, to methylation analysis and proton nuclear magnetic resonance spectroscopy. the smallest saccharide was shown to be a tetrasaccharide with the structure alpha-l-rhap-(1-2)-l-rha. the next saccharide, an octasaccharide, was show ... | 1984 | 6199198 |
| peyer's patch lymphoid follicle epithelial adherence of a rabbit enteropathogenic escherichia coli (strain rdec-1). role of plasmid-mediated pili in initial adherence. | escherichia coli (strain rdec-1) adheres to m cells of rabbit peyer's patch lymphoid follicle epithelium. the rdec-1 strain contains an 85 x 10(6) d plasmid that codes for pili, which, when purified, adhere to gut absorptive epithelium. this study compared the in vivo lymphoid follicle adherence of the rdec-1 strain with that of a shigella flexneri (shd15) that contained the 85 x 10(6) d plasmid and expressed the rdec-1 pili, a control e. coli, and a control s. flexneri (shd12). the bacteria wer ... | 1984 | 6145724 |
| [the conjugation system determined by plasmid r477 and other plasmids belonging to the h2 incompatibility group]. | 1984 | 6148780 | |
| [immunochemical analysis of oligosaccharide fragments of o-specific shigella flexneri polysaccharides]. | comparison of inhibitory properties of several synthetic oligosaccharides related to sh. flexneri o-specific polysaccharides, namely glc alpha 1-3rha alpha 1-ome, glc alpha 1-3rha alpha 1-2rha alpha 1-ome, rha alpha 1-2(glc alpha 1-3)rha alpha 1-ome, glcnac beta 1-2(glc alpha 1-3)rha alpha 1-ome, and glcnac beta 1-2(glc alpha 1-3) rha alpha 1-2rha alpha 1-ome, using passive haemagglutination reaction demonstrated the tetrasaccharide to possess the highest activity in v; 7,8-anti-7,8 immune syste ... | 1984 | 6083795 |
| glycocalyx visualization in shigella flexneri. | 1984 | 6085609 | |
| [in vitro activity of a new cephalosporin (ceftriaxone) on recently isolated strains of shigella]. | 1984 | 6088342 | |
| virulence of entamoeba histolytica trophozoites. effects of bacteria, microaerobic conditions, and metronidazole. | the association of axenically grown trophozoites of entamoeba histolytica strains hk-9 or hm-1:imss with various types of gram-negative bacteria for relatively short periods markedly increased their virulence, as evidenced by their ability to destroy monolayers of tissue-cultured cells. interaction of trophozoites with bacteria that were heat inactivated, glutaraldehyde fixed, or disrupted by sonication, or bacteria treated with inhibitors of protein synthesis, did not augment amebic virulence. ... | 1984 | 6088660 |
| cloning and characterization of reca genes from proteus vulgaris, erwinia carotovora, shigella flexneri, and escherichia coli b/r. | the reca genes of proteus vulgaris, erwinia carotovora, shigella flexneri and escherichia coli b/r have been isolated and introduced into escherichia coli k-12. all the heterologous genes restore resistance to killing by uv irradiation and the mutagen 4-nitroquinoline-1-oxide in reca- e. coli k-12 hosts. recombination proficiency is also restored as measured by formation of lac+ recombinants from duplicated mutant lacz genes and the ability to propagate phage lambda derivatives requiring host re ... | 1984 | 6090408 |
| aerobactin genes in shigella spp. | aerobactin, a hydroxamate iron transport compound, is synthesized by some, but not all, shigella species. conjugation and hybridization studies indicated that the genes for the synthesis and transport of aerobactin are linked and are found on the chromosome of shigella flexneri, s. boydii, and s. sonnei. the genes were not found in s. dysenteriae. a number of aerobactin synthesis mutants and transport mutants have been isolated. the most common mutations are deletions of the biosynthesis or bios ... | 1984 | 6090419 |
| mercuric ion-resistance operons of plasmid r100 and transposon tn501: the beginning of the operon including the regulatory region and the first two structural genes. | the mercuric ion-resistance operons of plasmid r100 (originally from shigella) and transposon tn501 (originally from a plasmid isolated in pseudomonas) have been compared by dna sequence analysis. the sequences for the first 1340 base pairs of tn501 are given with the best alignment with the comparable 1319 base pairs of r100. the homology between the two sequences starts at base 58 after the end of the insertion sequence is-1 of r100. the sequences include the transcriptional regulatory region, ... | 1984 | 6091128 |
| evidence for clustering of rna polymerase and ribosomal protein genes in six species of enterobacteria. | 32p-labelled dna fragments from the e. coli beta operon were used as hybridisation probes for homologous dna sequences in chromosomal digests of six species of enterobacteria. in all species the hybridisation pattern suggested clustering of the genes comprising the beta operon. | 1984 | 6092848 |
| temperature-dependent expression of virulence genes in shigella species. | the pathogenicity of shigella spp. involves the ability of the bacteria to penetrate and replicate within the epithelial cells of the large intestine. model systems for examining the virulence of shigellae employ henle intestinal epithelial cells in tissue culture and an in vivo assay for virulence in guinea pig eyes (sereny test). using these systems, we studied the genetic and physiological bases for the ability of shigellae to invade epithelial cells. we found that expression of virulence in ... | 1984 | 6360895 |
| loss of pigmentation in shigella flexneri 2a is correlated with loss of virulence and virulence-associated plasmid. | in this study, we examined the relationship between the virulence of shigella flexneri 2a and the ability of strains of s. flexneri 2a to absorb congo red. spontaneous nonpigmented (i.e., unable to bind congo red [pcr-]) derivatives of a virulent, pigmented (pcr+) strain of s. flexneri 2a were isolated and assayed for virulence as determined by their ability to invade epithelial cells. all pcr- mutants examined lost the ability to invade epithelial cells and were thus avirulent. agarose gel elec ... | 1984 | 6360906 |
| splenic abscess caused by shigella flexneri and bacteroides fragilis. | we report a case of splenic abscess from which only shigella flexneri (serotype 1a) and bacteroides fragilis were isolated. the patient was a 59-year-old diabetic female who displayed minimal gastrointestinal symptoms. the s. flexneri isolate was resistant to chloramphenicol and tetracycline. the possible synergistic relationship between the two organisms is discussed. | 1984 | 6361064 |
| entamoeba histolytica: virulence enhancement of isoenzyme-stable parasites. | pathogenic entamoeba histolytica isolated from patients with clinical amoebiasis can be differentiated from nonpathogenic e. histolytica obtained from asymptomatic carriers on the basis of the electrophoretic pattern of their isoenzymes. virulence of different strains of axenically grown trophozoites of entamoeba histolytica, as determined by various laboratory tests, such as damage to tissue culture monolayers, or their ability to cause an hepatic abscess in a hamster, are known to vary conside ... | 1984 | 6325226 |
| comparative efficacies of pivmecillinam and ampicillin in acute shigellosis. | the clinical efficacies of pivmecillinam and ampicillin were compared in a randomized double-blind trial in the treatment of acute shigellosis. of 44 adult male patients, all culture positive for shigella strains, 22 patients received 400 mg of pivmecillinam and 22 patients received 500 mg of ampicillin every 6 h. both drugs were administered orally for 5 days. four patients receiving ampicillin were infected with shigella strains that were resistant to ampicillin but susceptible to pivmecillina ... | 1984 | 6329092 |
| lipid a fractions analyzed by a technique involving thin-layer chromatography and enzyme-linked immunosorbent assay. | a modified enzyme-linked immunosorbent assay (elisa), with alkaline phosphatase as enzyme, was used for the study of antigenicity of lipid a fractions directly on thin-layer chromatographic (tlc) plates. for visualization a gel slab containing the enzyme substrate was placed on the plate containing enzyme-conjugated antibodies. the plate was read by a thin-layer chromatogram spectrophotometer. the immunoassay was both highly specific and quite sensitive. sensitivity was superior to levels obtain ... | 1984 | 6365543 |
| [food infection caused by shrimp]. | 1984 | 6366590 | |
| [chromosome markers of drug resistance as the selectors of potential vaccinal strains]. | mutations resulting in acquisition of the resistance to rifampicin, streptomycin, neamine, polymyxin m, mecillinam and high concentrations of nalidixic acid were studied with respect to their effect on virulence of shigella flexneri by transduction of the markers from the spontaneous avirulent mutants to the initial strain. it was shown that the acquisition of the determinants of the resistance to rifampicin, streptomycin, neamine (nea a) and high concentration of nalidixic acid resulted in 100 ... | 1984 | 6367629 |
| [transfer of resistance in shigellae to azlocillin and other antibiotics]. | 1984 | 6367893 | |
| r plasmids conferring multiple drug resistance from shigella isolated in korea. | the majority (85%) of shigella isolated in 1980 and 1981 in korea were shigella flexneri, the others were sh. sonnei (14%) with only a small number of sh. dysenteriae. only 14 of the 459 strains of shigella isolated were susceptible to all 12 drugs tested, and 445 were resistant to three or more drugs. strains multiply resistant to the six drugs, chloramphenicol (cm), tetracycline (tc), streptomycin (sm), sulfisomidine (su), ampicillin (ap) and trimethoprim (tp) were most frequently encountered, ... | 1984 | 6368684 |
| [preventive effectiveness of dried polyvalent shigella bacteriophage in organized collective farms]. | 1984 | 6235671 | |
| bacteriophage mu d1(apr lac) generates vir-lac operon fusions in shigella flexneri 2a. | previous studies have demonstrated that expression of virulence in shigella spp. is controlled by growth temperature. to study the regulation of virulence (vir) genes, we set out to develop a rapid, easily-assayed phenotype with which to measure expression of virulence. this report described a procedure for isolating vir-lac operon fusions in s. flexneri 2a by using the specialized transducing bacteriophage mu d1(apr lac) of casadaban and cohen (m. casadaban and s. n. cohen, proc. natl. acad. sc ... | 1984 | 6236150 |
| changes in the trend of shigellosis in dhaka: family study on secondary infection, clinical manifestation and sensitivity pattern: 1980. | the incidence of shigellosis and the death rate have increased and the resistance of shigellae to antibiotics has changed in dhaka during our experiences. in 1980, we investigated the secondary infection and case rates, infection to case ratio, duration of illness, excretion of shigellae and antibiotic sensitivity pattern in 100 families with cases of shigellosis, culturing rectal swabs obtained by home visits for a 10-day period. standard methods were used for culture and sensitivity tests. the ... | 1984 | 6380011 |
| [structure of a shigella flexneri population in the process of forming microcolonies]. | the study of the adaptation period of the s- and r-forms of s. flexneri in a nutritionally sufficient medium has revealed that the population structure characteristic of the s-form and ra-chemotype comprises two classes of cells: actively growing and nonviable. besides these two classes, the class of spontaneously lysing bacteria is present in the population of mutants with various degrees of lipopolysaccharide (lps) lesions. the number of bacteria subject to autolysis increases with the progres ... | 1984 | 6380164 |
| purification and properties of carboxylesterase b of escherichia coli. | carboxylesterase b produced by escherichia coli was purified 1,350-fold with a recovery of 12% by successive gel filtrations, deae-trisacryl, phenyl-sepharose chromatography and preparative electrophoresis. the purified enzyme was found to be homogeneous, as judged by a single precipitation line in ouchterlony double diffusion in an experiment with homologous antiserum. the apparent molecular weight determined by gel filtration and the isoelectric point determined by electrofocusing were 57,000 ... | 1984 | 6380369 |
| cytotoxicity of human peripheral blood and colostral leukocytes against shigella species. | we examined the ability of human peripheral blood leukocytes to kill strains of shigella sonnei and shigella flexneri by using a modified bactericidal assay. antibody-dependent cellular cytotoxicity (adcc) was demonstrated in the presence of specific rabbit immune serum directed against s. sonnei. with peripheral blood leukocytes from adults, adcc was found only in the mononuclear cell and purified lymphocyte populations. monocyte-macrophages and polymorphonuclear leukocytes were unable to demon ... | 1984 | 6384045 |
| heterogeneity of lipid a: comparison of lipid a types from different gram-negative bacteria. | chloroform-soluble purified lipid a preparations from 10 sources, including five escherichia coli strains (eh100, k-12, o127, o111, rcdc), two salmonella strains (salmonella typhimurium, salmonella minnesota r595), shigella sonnei ii, and a hybrid of shigella flexneri and e. coli k-12, were compared with lipid a from s. flexneri. purified lipid a from s. flexneri was earlier found to be composed of eight fractions. the various lipid a preparations were assayed by thin-layer chromatography. chrom ... | 1984 | 6384185 |
| effect of cytochalasin b and dihydrocytochalasin b on invasiveness of entero-invasive bacteria in hep-2 cell cultures. | the effect of cytochalasin b (cb) and di-hydrocytochalasin b (h2cb) on the invasiveness of salmonella typhimurium, shigella flexneri and yersinia enterocolotica serotype 0:3 in hep-2 cell cultures was examined. the intra-cellular and extra-cellular bacteria were identified by a combination of nomarski interference contrast microscopy and uv incident light microscopy applied on the same light microscope. pre-treatment of cells with cb and h2cb inhibited the uptake of s. typhimurium and s. flexner ... | 1984 | 6385614 |
| usefulness of plasmid profiles for differentiation of shigella isolates in bangladesh. | we studied the plasmid profiles of 136 shigella isolates in bangladesh to determine whether plasmid profiles could be used for differentiation of strains for epidemiological studies. many different plasmid patterns were observed within each species, indicating that many genetically different strains of shigella are responsible for illness in bangladesh. | 1984 | 6386850 |
| oral vaccination of monkeys with an invasive escherichia coli k-12 hybrid expressing shigella flexneri 2a somatic antigen. | a living oral vaccine, designed to protect against shigella flexneri 2a infections, was constructed by using escherichia coli k-12 as a carrier strain. the hybrid strain, designated ec104, contained both chromosomal and plasmid genes from s. flexneri donor strains. in addition to expressing the s. flexneri 2a somatic antigen, it had inherited the property of epithelial-cell invasion. after the oral administration to rhesus monkeys, ec104 was isolated from the feces for up to 3 days, but by day 4 ... | 1984 | 6389344 |
| the prevalence of gastro-intestinal parasites and bacteria in the game scouts at the kainji lake national park of nigeria. | the faeces of twenty scouts working at borgu games were examined for the presence of parasites and bacteria. cysts of entamoeba histolytica, giardia lamblia and trichomonas hominis were found. helminth ova encountered included those of ancylostoma duodenale, ascaris lumbricoides, stronglyloides stercoralis and trichuris trichiura. escherichia coli and shigella flexneri were isolated from one faecal sample each. in all, parasites were found in the faeces of only 9 scouts. the epidemiological sign ... | 1984 | 6389403 |
| [bacillary dysentery 1982]. | 1984 | 6390523 | |
| [clinical and treatment characteristics of acute dysentery caused by shigella boydii and newcastle]. | 1984 | 6390958 | |
| [further decrease in intestinal infection morbidity in the ukrainian ssr]. | 1984 | 6390962 | |
| [conditions and factors determining the etiological structure of dysentery and its alterations. ii. concepts, hypotheses and points of view yesterday and today]. | 1984 | 6391040 | |
| [conditions and factors determining the etiological structure of dysentery and its alterations. iii. the epidemiological assessment of the biological properties of shigella and their importance in the development of the epidemic process in different nosological forms of dysentery]. | 1984 | 6391041 | |
| dna hybridization technique to detect shigella species and enteroinvasive escherichia coli. | a dna hybridization method for the detection of shigella species and enteroinvasive escherichia coli is described. it is based upon the high degree of homology shared by the virulence plasmids present in all pathogenic strains. after 32p labeling, a 17-kilobase ecori restriction fragment of a virulence plasmid belonging to shigella flexneri serotype 5 was used as a probe to detect shigella species and enteroinvasive e. coli in isolated colonies. this method proved highly specific as well as sens ... | 1984 | 6392327 |
| shigella vulvovaginitis in a three-year-old child: a case report. | 1984 | 6392838 | |
| [identification of specific antibodies in dysentery]. | 1984 | 6393359 | |
| shigellemia. | 1984 | 6394497 | |
| association of pneumonia with undernutrition and shigellosis. | 1984 | 6394498 | |
| the effect of postnatal age on the adherence of shigella flexneri, escherichia coli 0124, and e. coli 0128 to guinea pig intestinal cells. | since postnatal development of the gastrointestinal tract has an important effect on its microbial flora and may influence the types of intestinal infections, we examined the effect of age on bacterial adherence to intestinal epithelial cells. radiolabeled bacteria were incubated with guinea pig enterocytes released by treating loops of the intestine with solutions containing edta, dithiothreitol, and citrate. nonbound bacteria were separated from intestinal cells by sedimentation on a percoll g ... | 1984 | 6395082 |
| [species of shigella isolated in a 4-year period and antimicrobial sensitivity]. | 1984 | 6395307 | |
| [structure of the causative agents of shigellosis in areas of the ussr 1979-1981]. | summarized data on the specific structure of shigella strains isolated in different territories of the ussr in 1979-1981 are presented. these data indicate that in the intra-specific structure of s. flexneri strains the proportion of serological subvariant 2a increased and that of serological variant 6 and subvariants 16 and 4a decreased during this period. using one of the base territories of the all-union shigellosis center as an example, the spread of an unusual serological variant of s. flex ... | 1984 | 6395590 |
| [cathepsin d activity in splenocytes in relation to shigella virulence in an experiment]. | the overtime study of changes in the activity of cathepsin d, a lysosomal enzyme, in the splenocytes of cba mice after their infection with virulent and avirulent shigella strains of the same origin and with the same antigenic structure has been made. as the result of two months of observations, changes in the activity of this enzyme in the cytoplasmic and lysosomal cell fractions have been found to occur in phases. the activity of cathepsin d has been shown to depend on the virulence of shigell ... | 1984 | 6395592 |
| shigellosis: an old disease with new faces. | 1984 | 6397507 | |
| can erythema nodosum and reactive arthritis be a sequel to shigella flexneri gastroenteritis? | erythema nodosum and seronegative arthritis associated with diarrhoea was previously regarded as implying the presence of inflammatory bowel disease. recently, however, erythema nodosum has been described as a sequel to diarrhoea caused by infection with salmonella typhimurium, yersinia enterocolitica and campylobacter jejuni. we report two patients in whom erythema nodosum and reactive arthritis followed acute shigella flexneri gastroenteritis, a rare association. | 1984 | 6398514 |