Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| recommendations for use of the polymerase chain reaction in the diagnosis of bordetella pertussis infections. | 1994 | 7911841 | |
| lewis antigen expression on human monocytes and binding of pyrogenic toxins. | toxigenic bacteria such as bordetella pertussis and staphylococcus aureus have been implicated in some cases of sudden infant death syndrome (sids). we have previously demonstrated that the lewis(a) antigen is an epithelial cell receptor for s. aureus, and this study demonstrated that lewis(a) on human monocytes is also a receptor for staphylococcal enterotoxin b (seb). values obtained in assays for production of tnf-alpha and nitric oxide were greater for monocytes treated with seb compared wit ... | 1994 | 7915870 |
| update of the rapid diagnosis of infectious diseases. i: bacteria, fungi and parasites. | the application of monoclonal antibodies and dna probes in the clinical microbiology laboratory has resulted in an array of rapid diagnostic tests. the immunofluorescent assay or enzyme-linked immunoassay is widely used in the rapid diagnosis of bacteria eg group a streptococcus, legionella pneumophila, mycoplasma pneumoniae, bordetella pertussis; parasites eg chlamydia tachomatis, cryptosporidium species; and fungi eg pneumocystis carinii. the bactec system was first introduced to detect bacter ... | 1994 | 7997914 |
| analysis of immunogenic properties of nonapeptide tvgrgdphq from bordetella pertussis filamentous hemagglutinin. | immunogenic properties of tvgrgdphq nonapeptide which is correspondent to the region 1094-1102 of b. pertussis filamentous hemagglutinin (fha) were studied. the conjugate of bovine serum albumin with nonapeptide was used for immunization of balb/c and cba mice. antisera of the both lines of mice cross-reacted with a number of antigens, but using affinity chromatography peptide and fha specific antibodies were extracted. affinity purified rabbit antibodies to tvgrgphq which recognize fha were als ... | 1994 | 7998347 |
| characterization of murine lung inflammation after infection with parental bordetella pertussis and mutants deficient in adhesins or toxins. | bordetella pertussis expresses factors such as filamentous hemagglutinin, agglutinogens, pertactin, and pertussis toxin, which participate in bacterial adhesion; pertussis toxin, dermonecrotic toxin, lipopolysaccharide, and tracheal cytotoxin, which are responsible for toxic effects; and adenylate cyclase-hemolysin, which is required to initiate infection. by using a murine respiratory model, we showed that the rgd sequences of filamentous hemagglutinin and pertactin are important for bacterial ... | 1994 | 7999145 |
| epithelial cell invasion and survival of bordetella bronchiseptica. | wild-type bordetella bronchiseptica and a bvg mutant strain were used for invasion and survival experiments in human caco-2 and a549 epithelial cells. both bacterial strains were able to enter and persist within the host cells for at least a week. a significant proportion of the bacteria from both b. bronchiseptica strains but not from bordetella pertussis were found free in the cytoplasm, suggesting different invasion and survival strategies of the two species in epithelial cells. | 1994 | 8005690 |
| bordetella pertussis diagnosed by polymerase chain reaction. | the object of this work was to test the polymerase chain reaction (pcr) for demonstration of bordetella pertussis (bp) in nasopharyngeal secretions. the method was applied to patients with recently diagnosed pertussis, as verified by bp culture. in order to test the sensitivity and specificity of pcr for the diagnosis of bp, we used known concentrations of bp, bordetella parapertussis and bordetella bronchiseptica in aqueous solutions. pcr was furthermore carried out on species of bacteria that ... | 1994 | 8011307 |
| inhibition of pcr-based assay for bordetella pertussis by using calcium alginate fiber and aluminum shaft components of a nasopharyngeal swab. | a pcr-based assay for bordetella pertussis was inhibited by using a calcium alginate fiber-tipped swab with an aluminum shaft but not by using a dacron fiber-tipped swab with a plastic shaft. the calcium alginate fiber component inhibited the assay following storage for less than 1 min in a suspension of 10(3) cfu of b. pertussis per ml, whereas the aluminum shaft component required storage for at least 48 h in order to cause inhibition. we recommend the dacron swab over the calcium alginate swa ... | 1994 | 8027309 |
| identification of bordetella pertussis in nasopharyngeal swabs using the polymerase chain reaction: evaluation of detection methods. | a 183 base pairs or 153 base pairs dna fragment from a repetitive region of the bordetella pertussis genome was amplified in a polymerase chain reaction. the sensitivities of three different detection methods (enzymun test, silver stained polyacrylamide gel, ethidium bromide stained agarose gel) after amplification by polymerase chain reaction showed that both a one-time polymerase chain reaction (35 cycles) with enzymun testing as well as a nested polymerase chain reaction with either of the el ... | 1994 | 8031967 |
| clinical characteristics of illness caused by bordetella parapertussis compared with illness caused by bordetella pertussis. | in conjunction with a pertussis vaccine efficacy trial in germany, nasopharyngeal specimens were collected from may, 1992, to march, 1993, from patients with cough illnesses. clinical data were obtained by initial and follow-up questionnaires. bordetella parapertussis was isolated from 38 patients (mean age, 3.5 years; 68% girls). clinical characteristics in these cases were compared with those of 76 patients (matched by age and sex) with illness caused by bordetella pertussis during the same pe ... | 1994 | 8036048 |
| genes encoding high-molecular-weight adhesion proteins of nontypeable haemophilus influenzae are part of gene clusters. | we previously reported the cloning and sequencing of genes designated hmw1 and hmw2 from a prototype nontypeable haemophilus influenzae strain. the genes encode proteins which are related to filamentous hemagglutinin of bordetella pertussis and promote attachment of the nontypeable h. influenzae strain to human epithelial cells (j. w. st. geme iii, s. falkow, and s. j. barenkamp, proc. natl. acad. sci. usa 90:2875-2879, 1993). subcloning studies suggested that correct processing of these high-mo ... | 1994 | 8039903 |
| bvgas-mediated signal transduction: analysis of phase-locked regulatory mutants of bordetella bronchiseptica in a rabbit model. | members of the bordetella genus alternate between two distinct phenotypic phases in response to changes in their environment. this switch, termed phenotypic modulation, is mediated by the bvgas sensory transduction system. we developed an animal model based on the interaction of bordetella bronchiseptica with one of its natural hosts, the rabbit. to investigate the importance of bvgas signal transduction, we constructed constitutive (rb53) and bvg- (rb54) phase-locked derivatives of a wild-type ... | 1994 | 8039908 |
| structure of a hexasaccharide proximal to the hydrophobic region of lipopolysaccharides present in bordetella pertussis endotoxin preparations. | a branched-chain hexasaccharide containing 3-deoxy-d-manno-oct-2-ulosonic acid was released by detergent-promoted hydrolysis from bordetella pertussis endotoxin preparations that were first dephosphorylated with aqueous hf and then treated with nitrous acid. its structure (2) [formula: see text] was determined by chemical and physical methods. this hexasaccharide is present in all four lipopolysaccharides that make up the b. pertussis strain 1414 (phase 1) endotoxin preparations analysed, and is ... | 1994 | 8050099 |
| activation of gi protein by peptide structures of the muscarinic m2 receptor second intracellular loop. | the muscarinic m2 receptor that normally couples via gi to inhibit adenylyl cyclase was made to couple to gs by exchange of its third intracellular loop for the comparable domain of the beta 2-adrenoceptor. in hela cells transfected with the recombinant m2 beta i-3 cdna, the chimaeric receptor showed carbachol-mediated activation of adenylyl cyclase (ec50 = 73 nm) that was blocked by atropine, but not by propranolol. the chimaeric receptor was shown to mediate a carbachol-stimulated, bordetella ... | 1994 | 8050479 |
| structural characterization of the lipid a of bordetella pertussis 1414 endotoxin. | the structure of bordetella pertussis 1414 lipid a was investigated by classical methods of chemical analysis as well as plasma desorption mass spectrometry and fast atom bombardment mass spectrometry. previous analysis showed that it contained a bisphosphorylated beta-(1-->6)-linked d-glucosamine disaccharide with hydroxytetradecanoic acid in amide linkage. the presence of two main molecular species as seen by thin-layer chromatography was confirmed by plasma desorption mass spectrometry, in wh ... | 1994 | 8051033 |
| mutations in the bordetella pertussis bvgs gene that confer altered expression of the fhab gene in escherichia coli. | the bvg locus of bordetella pertussis, required for coordinate regulation of virulence genes in response to environmental signals, encodes two proteins, bvgs and bvga, that belong to the bacterial two-component signal transduction systems. we have isolated spontaneous mutations of the bvg locus in escherichia coli and analyzed their effects on the expression of fhab::laczy transcriptional fusions. the mutations, localized in the linker and transmitter domain of bvgs, result in increased activati ... | 1994 | 8051035 |
| characterization of the dermonecrotic toxin in members of the genus bordetella. | all members of the genus bordetella and pasteurella multocida (a gram-negative bacillus genetically unrelated to bordetella spp., yet often sharing the same ecological niche) produce a dermonecrotic toxin (dnt). the amount of toxin produced and the time required for appearance of the lesions are identical for bordetella pertussis, b. parapertussis, and b. bronchiseptica but different for p. multocida and b. avium. dnt has been reported to act by promoting vasoconstriction; however, vasoactive co ... | 1994 | 8063398 |
| shuttle mutagenesis of legionella pneumophila: identification of a gene associated with host cell cytopathicity. | we performed shuttle mutagenesis of legionella pneumophila. mutants were screened for reduced cellular infectivity. approximately 10% of the mutants had decreased cytopathicity. the dna sequence of one locus was determined; the inferred amino acid sequence revealed homology with transport proteins including escherichia coli tolc, bordetella pertussis cyae, and alcaligenes eutrophus czcc and cnrc. | 1994 | 8063428 |
| the modular architecture of bacterial response regulators. insights into the activation mechanism of the bvga transactivator of bordetella pertussis. | control of virulence factor expression in bordetella pertussis is mediated by the products of the bvg operon. the bvgs membrane protein responds to certain environmental cues by activating the bvga protein, which in turn modulates the expression of the target virulence factor genes. the bvga and bvgs proteins are members of a large family of sensory transduction proteins called the two-component systems. we show that bvga fusion proteins can activate transcription of a reporter gene containing t ... | 1994 | 8064853 |
| surveillance for bordetella pertussis infection in victoria. | our aims were to describe the epidemiology of bordetella pertussis infection in victoria during the last decade and to evaluate surveillance of b. pertussis by comparing notifications with laboratory isolations and hospital diagnoses. whooping cough was once a leading cause of childhood morbidity and mortality but there was a dramatic reduction in the 1940s because of immunisation. during the last two decades, controversy about the vaccine's toxicity has resulted in waning immunisation rates and ... | 1994 | 8068787 |
| bordetella pertussis as a cause of chronic respiratory infection in an aids patient. | a 60-year-old heterosexual man with aids was admitted to hospital with dyspnea, a severe paroxysmal non-productive cough of two months' duration, low-grade fever and exhaustion. bordetella pertussis was cultured from a bronchoalveolar lavage specimen. after erythromycin therapy (500 mg q.i.d. for two weeks) all respiratory symptoms resolved progressively over a four-week period. bordetella pertussis should be added to the long list of pathogens that may cause respiratory disease in persons with ... | 1994 | 8070437 |
| the product of the virb4 gene of agrobacterium tumefaciens promotes accumulation of virb3 protein. | the process of t-dna transfer from agrobacterium tumefaciens to plant cells is thought to involve passage of a dna-protein complex through a specialized structure in the bacterial membrane. the virb operon of a. tumefaciens encodes 11 proteins, of which 9 are known to be located in the membranes and 10 have been shown to be essential for virulence. sequence comparisons between proteins encoded by the virb operon and those encoded by operons from conjugative plasmids indicated that virb proteins ... | 1994 | 8071199 |
| detection and subcellular localization of three ptl proteins involved in the secretion of pertussis toxin from bordetella pertussis. | the ptl locus of bordetella pertussis contains eight open reading frames which are predicted to encode proteins (ptla to ptlh) that are essential for secretion of pertussis toxin from the bacterium and which are members of a family of transport proteins found in other types of bacteria. we have detected ptle, ptlf, and ptlg in immunoblots of extracts of b. pertussis by using antibodies raised to fusion proteins consisting of maltose-binding protein and the individual ptl proteins. these proteins ... | 1994 | 8071211 |
| the crystal structure of pertussis toxin. | pertussis toxin is an exotoxin of the a-b class produced by bordetella pertussis. the holotoxin comprises 952 residues forming six subunits (five different sequences, s1-s5). it plays an important role in the development of protective immunity to whooping cough, and is an essential component of new acellular vaccines. it is also widely used as a biochemical tool to adp-ribosylate gtp-binding proteins in the study of signal transduction. | 1994 | 8075982 |
| adenylate cyclase toxin from bordetella pertussis produces ion conductance across artificial lipid bilayers in a calcium- and polarity-dependent manner. | adenylate cyclase toxin (ac toxin) from bordetella pertussis enters target cells to produce supraphysiologic levels of camp and, by a camp-independent process, is hemolytic. in the present study, we show for the first time that this toxin also produces ion-permeable, cation-selective pores in phospholipid bilayers. the resulting membrane conductance is absolutely calcium-dependent, as are the intoxication and hemolytic activities. it is strongly affected by the polarity and magnitude of the memb ... | 1994 | 8077197 |
| viability of bordetella pertussis in four suspending solutions at three temperatures. | we studied the survival of bordetella pertussis in four suspending solutions (casamino acids broth, deionized water, phosphate-buffered saline, and serum inositol), subjected to three storage temperatures (4, -20, and -70 degrees c) and two freezing methods (direct freezing and fast-freezing in an ethanol-dry-ice bath). recovery rates were higher for longer periods for suspensions stored at -70 degrees c than those stored at -20 or 4 degrees c. serum inositol showed the highest recovery rates fo ... | 1994 | 8077402 |
| antibodies to filamentous hemagglutinin of bordetella pertussis and protection against whooping cough in schoolchildren. | a pertussis outbreak was studied prospectively in an elementary school with 39 pupils. all had been immunized with at least three doses of finnish diphtheria-tetanus toxoid-pertussis vaccine. diagnosis of pertussis was based on culture, polymerase chain reaction results, and eia serology using filamentous hemagglutinin (fha), pertussis toxin, and 69-kda outer membrane protein as antigens. at the first sampling, 21 children had symptoms suggestive of pertussis, and 18 were healthy. of the latter, ... | 1994 | 8077734 |
| effects of thermocyclers and primers on the reproducibility of banding patterns in randomly amplified polymorphic dna analysis. | the effects of thermocyclers and primers on the reproducibility of banding patterns in randomly amplified polymorphic dna analysis were tested. purified bordetella pertussis dna was analysed with four primers (12-mer), which did not differ from each other in their gc-content. three different thermocycler models from two manufacturers were tested. three of the primers produced consistent banding patterns in separate wells of the reaction plates of individual thermocyclers, and in different thermo ... | 1994 | 7935514 |
| internal lysine palmitoylation in adenylate cyclase toxin from bordetella pertussis. | a number of bacterial protein toxins, including adenylate cyclase (ac) toxin from bordetella pertussis, require the product of an accessory gene in order to express their biological activities. in this study, mass spectrometry was used to demonstrate that activated, wild-type ac toxin was modified by amide-linked palmitoylation on the epsilon-amino group of lysine 983. this modification was absent from a mutant in which the accessory gene had been disrupted. a synthetic palmitoylated peptide cor ... | 1994 | 7939682 |
| [an investigation of factors in the pathogenesis of experimental autoimmune uveoretinitis (eau) in congenic mice]. | s-antigen or interphotoreceptor retinoid-binding protein (irbp), when injected with freund's complete adjuvant into mice, does not easily cause experimental autoimmune uveoretinitis (eau). in this report, we describe the results of injecting irbp with freund's complete adjuvant, together with the intraperitoneal administration of bordetella pertussis, into several types of congenic mice (b10, b10a, b10br, b10d2). these congenic mice, of c57bl/10 (b10) origin, differ at the h-2 locus on chromosom ... | 1994 | 7942337 |
| yersinia-specific antibodies in serum and synovial fluid in patients with yersinia triggered reactive arthritis. | to further evaluate the role of bacterial antigens in triggering inflammation in the joint in patients with reactive arthritis by studying local antibody synthesis in the joint. | 1994 | 7944640 |
| immunoelectron microscopy of antigens of bordetella pertussis using monoclonal antibodies to agglutinogens 2 and 3, filamentous haemagglutinin, pertussis toxin, pertactin and adenylate cyclase toxin. | immunogold electron microscopy and monoclonal antibodies (mabs) were used to localize surface-related antigens of bordetella pertussis. unfixed organisms of b. pertussis strains which are included in the danish whole-cell pertussis vaccine and fixed cells from a vial of vaccine were examined. mabs to agglutinogens 2 and 3 labelled fimbria-like structures on both live and fixed cells in a serotype-specific manner. mab against pertactin, a 69 kda outer membrane protein, produced intense labelling ... | 1994 | 7946271 |
| primers are decisive for sensitivity of pcr. | a sufficient sensitivity of pcr is a prerequisite for its use in the diagnosis of infectious diseases. we have used pcr for detecting gene elements of borrelia burgdorferi, mycobacteria and bordetella pertussis. with all these microbe groups, difficulties were encountered in achieving the demanded sensitivity with the primer pairs primarily selected. an extensive testing of various reaction parameters did not improve the sensitivity. subsequently, we synthesized more primers derived from slightl ... | 1994 | 7946322 |
| activation of phospholipase d by interleukin-8 in human neutrophils. | interleukin 8 (il-8), a member of the c-x-c branch of the chemokine superfamily, stimulated the breakdown of 1-o-[3h]alkyl-2-acyl-sn-glycero-3-phosphocholine ([3h]eapc) and the formation of 1-o-[3h]alkyl-2-acyl-phosphatidic acid ([3h]-eapa) in human polymorphonuclear leukocytes (pmn) in the presence of cytochalasin b. in addition, the mass of diradyl-pa was increased with similar kinetics. in the presence of ethanol, 1-o-[3h]alkyl-2-acyl-phosphatidylethanol ([3h]eapet) was formed at the expense ... | 1994 | 7949145 |
| recombinant thyrotropin receptor and the induction of autoimmune thyroid disease in balb/c mice: a new animal model. | in a preliminary study, we observed the production of tsh binding-inhibiting (tbii) and thyroid-blocking (tbab) antibodies accompanied by lymphocytic infiltration of the thyroid in a pool of male balb/c mice immunized with the extracellular domain (ecd) of the human tsh receptor (tshr) expressed as a maltose-binding protein (mbp) fusion in bacteria. in the present study we evaluated the humoral response to the same antigenic preparation in a new series of individual male and female balb/c mice i ... | 1994 | 7956939 |
| role of pertussis toxin in causing symptoms of bordetella parapertussis infection. | whooping cough can be caused by either bordetella pertussis or bordetella parapertussis. although the two species share an almost complete dna identity, bordetella parapertussis does not produce pertussis toxin, which is thought to be the main virulence factor of bordetella pertussis. in order to elucidate the role of pertussis toxin in causing the typical symptoms of whooping cough, clinical information from 33 patients with culture-positive bordetella parapertussis infection was collected and ... | 1994 | 7957264 |
| mutations in the linker region of bvgs abolish response to environmental signals for the regulation of the virulence factors in bordetella pertussis. | expression of virulence factors of bordetella pertussis is coordinately regulated by the products of the bvg locus, which codes for a sensory protein (bvgs) and a positive regulator of transcription (bvga), a pair in the family of bacterial 'two-component' regulators. transcription of the bvg-regulated promoters is repressed by modulating environmental factors such as 50 mm mgso4, 10 mm nicotinic acid (na) or low temperature (25 degrees c). we have isolated a spontaneous mutant (sk170) which exp ... | 1994 | 7959037 |
| invasion and intracellular survival of bordetella bronchiseptica in mouse dendritic cells. | we have studied the interaction between the respiratory pathogen bordetella bronchiseptica and murine spleen dendritic cells, important antigen-presenting cells that are found in the airway epithelium. wild-type b. bronchiseptica 5376 attached very efficiently to dendritic cells, whereas the bvg mutant atcc 10580, wild-type strain bb7865, and its spontaneous delta bvgs mutant bb7866 bound less efficiently. however, all tested b. bronchiseptica strains were able to invade dendritic cells and surv ... | 1994 | 7960135 |
| hfr mapping of mutations in bordetella pertussis that define a genetic locus involved in virulence gene regulation. | we report the development of techniques for the genetic mapping of point mutations in the bacterial pathogen bordetella pertussis. a plasmid vector which is self-transmissible by conjugation and which, by insertion into the b. pertussis chromosome, can mobilize chromosomal sequences during conjugation with a recipient b. pertussis bacterium has been constructed. this vector is used in conjunction with a set of strains containing kanamycin resistance gene insertions at defined physical locations ... | 1994 | 7961497 |
| effect of mutations causing overexpression of rna polymerase alpha subunit on regulation of virulence factors in bordetella pertussis. | in bordetella pertussis, expression of virulence factors is controlled by the bvg proteins, which comprise a sensor-regulator two-component signal transduction system. previously, we described a mutant strain of b. pertussis that had reduced transcription of pertussis toxin and adenylate cyclase toxin genes, while other virulence factors were relatively unaffected. we obtained a b. pertussis clone that repaired the defect in both this strain and an independent mutant strain with a similar phenot ... | 1994 | 7961498 |
| circulating fibronectin and fibronectin receptor in children with pertussis. | to determine concentrations of fibronectin and fibronectin receptor in children with pertussis. | 1994 | 7962645 |
| erythromycin-resistant bordetella pertussis--yuma county, arizona, may-october 1994. | in 1993, a total of 6586 cases of pertussis was reported in the united states, including 70 in arizona. on june 27, 1994, a case of bordetella pertussis disease caused by a strain resistant to erythromycin was reported to the arizona department of health services (adhs) from yuma county (1990 population: 106,895). susceptibility testing at cdc confirmed that the isolate was highly resistant to erythromycin with a minimum inhibitory concentration (mic) > 64 micrograms/ml. the mic of erythromycin ... | 1994 | 7968996 |
| inhibition of anaphylactic shock by gadolinium chloride-induced kupffer cell blockade. | data in the literature concerning the role of macrophages in anaphylaxis are contradictory. in the present study, the effect of macrophage blockade induced by gadolinium chloride (gdcl3) on anaphylactic shock is investigated. our observations show that gdcl3 prevents lethal anaphylactic shock in mice sensitized to ovalbumin. gadolinium chloride given i.v. in a dose of 1 mg/100 g body weight 24 or 48 h before the elicitation of anaphylactic shock resulted in 80% survival, compared with the 43% su ... | 1994 | 7976819 |
| pertussis toxin stimulates hypersensitivity and enhances nerve-mediated antigen uptake in rat intestine. | we previously reported that intestine from rats sensitized to ovalbumin (ova), using bordetella pertussis vaccine as adjuvant, demonstrated a rapid secretory response [increase in short-circuit current (isc)] to ova upon secondary challenge. here, we examined the role of pertussis toxin, the active component of the vaccine, in the response. sensitization of sprague-dawley rats by intraperitoneal injection of recombinant wild-type pertussis toxin (wpt) plus ova enhanced intestinal responses (at d ... | 1994 | 7977735 |
| effect of erythromycin treatment on antibody responses in pertussis. | the effect of erythromycin treatment on antibody responses to bordetella pertussis filamentous haemagglutinin (fha) and pertussis toxin (pt) was investigated in convalescent blood samples from 105 children with pertussis. erythromycin had been given to 59 children, median age 3.2 years (range 0.3-9.9) on median day 7 (range 11-14) after onset of disease while the remaining 46 children, age 3.45 (0.6-8.1) were untreated. no significant differences in igg antibody concentration were noted to fha b ... | 1994 | 7984978 |
| d2 inhibition of stimulated fos immunoreactivity in cultured tyrosine hydroxylase-ir hypothalamic neurons. | we have previously demonstrated that fos immunoreactivity can be stimulated by kcl, forskolin or glutamate in cultured tyrosine hydroxylase-immunoreactive (th-ir) hypothalamic neurons. the present study was performed to determine whether agents that regulate dopaminergic activity, particularly d1 and d2 receptor agonists, modulate the intracellular cascade leading to fos expression. dissociated hypothalamic cultures were prepared from neonatal rats. the cultures were treated with d1- or d2-speci ... | 1994 | 7922580 |
| monocyte chemotactic proteins mcp-1, mcp-2, and mcp-3 are major attractants for human cd4+ and cd8+ t lymphocytes. | the responses of lymphocytes to six cc chemokines--mcp-1, mcp-2, mcp-3, mip-1 alpha, mip-1 beta, and rantes--were studied using cloned human cd4+ and cd8+ t cells. all cc chemokines tested induced migration of both types of lymphocytes, whereas two cxc chemokines used as controls, il-8 and ip-10, were inactive. the monocyte chemotactic proteins (mcp-1, mcp-2, and mcp-3) showed a typically bimodal concentration dependence, and were considerably more effective than mip-1 alpha, mip-1 beta, or rant ... | 1994 | 7926371 |
| [enhanced antibody production by lung lymphocytes after oral immunization with bordetella pertussis surface antigens]. | the purpose of our study was to evaluate the effect of oral vaccination with bordetella pertussis surface antigens on the immune response at the site of antigen application. we orally immunized female balb/c mice on five consecutive days and repeated this procedure after a free interval of 10 days. lymphocytes of the lung (ll), peyer's patches (ppl) and lamina propria of the gut (lpl) were isolated and the immunoglobulin secretion rate was measured with time-resolved immunofluorescence. oral imm ... | 1994 | 7927468 |
| surface-associated filamentous hemagglutinin induces autoagglutination of bordetella pertussis. | filamentous hemagglutinin (fha) is a major adhesin produced by bordetella pertussis, the etiologic agent of whooping cough. fha has been shown to be surface associated but is also secreted by virulent bacteria. microscopic observations of lungs of mice infected with b. pertussis showed that the bacteria grow as clusters within the alveolar lumen. when b. pertussis was cultivated in vitro with chemically defined medium, bacteria grew as aggregates, mimicking growth observed in vivo. this aggregat ... | 1994 | 7927683 |
| localization of high-molecular-weight adhesion proteins of nontypeable haemophilus influenzae by immunoelectron microscopy. | a family of high-molecular-weight (hmw) surface-exposed proteins important in the attachment of nontypeable haemophilus influenzae (nthi) to human epithelial cells was previously identified (j. w. st. geme iii, s. falkow, and s. j. barenkamp, proc. natl. acad. sci. usa 90:2875-2879, 1993). in the present investigation, indirect immunogold labeling and electron microscopy were used to localize these proteins on three clinical isolates of nthi, mutants deficient in expression of one or both hmw pr ... | 1994 | 7927710 |
| cloning and sequencing of a bordetella pertussis serum resistance locus. | we have characterized a new virulence factor in bordetella pertussis: serum resistance. compared with escherichia coli hb101, wild-type b. pertussis was relatively resistant to classical-pathway, complement-dependent killing by normal human serum. however, a mutant of b. pertussis (bpm2041) which is less virulent in mice and which has tn5 lac inserted in a previously uncharacterized bvg-regulated gene was found to be at least 10-fold more susceptible to serum killing than the wild type. we have ... | 1994 | 7927748 |
| virulence factors determine attachment and ingestion of nonopsonized and opsonized bordetella pertussis by human monocytes. | in the present study, the role of virulence factors in and the effect of opsonization on the interactions between bordetella pertussis and human monocytes were investigated. the methods used facilitated the distinction between attachment and ingestion of bacteria by monocytes. nonopsonized virulent b. pertussis cells attached to monocytes. nonopsonized b. pertussis mutant strains deficient in filamentous hemagglutinin, fimbriae, or pertactin exhibited a reduced adherence to monocytes compared wi ... | 1994 | 7927760 |
| sulfated glycoconjugate receptors for the bordetella pertussis adhesin filamentous hemagglutinin (fha) and mapping of the heparin-binding domain on fha. | filamentous hemagglutinin (fha) is a major adhesin present on the surface of the gram-negative respiratory pathogen bordetella pertussis. a number of binding mechanisms have been described for the interaction of fha with eukaryotic cells. we have focused on its function as a sulfated polysaccharide-binding protein and on identifying potential receptors for fha on the epithelial cell surface. using a thin-layer overlay technique, we found that fha binds specifically to sulfated glycolipids but no ... | 1994 | 7927782 |
| outcomes of bordetella pertussis infection in different age groups of an immunized population. | outcomes of bordetella pertussis infection were studied in 3 age groups (1-3, 4-6, and 7-15 years) during outbreaks in one day care center (n = 29) and in two elementary schools (n = 210). a total of 76 children were confirmed as having b. pertussis infection; 74 were confirmed by polymerase chain reaction (pcr) and 18 by culture. a positive pcr result was less common in children 1-3 years old than in those 4-6 (p = .006). asymptomatic b. pertussis infection was more common in preschool children ... | 1994 | 7930729 |
| bordetella pertussis filamentous hemagglutinin interacts with a leukocyte signal transduction complex and stimulates bacterial adherence to monocyte cr3 (cd11b/cd18). | bordetella pertussis, the causative agent of whooping cough, adheres to human monocytes/macrophages by means of a bacterial surface-associated protein, filamentous hemagglutinin (fha) and the leukocyte integrin, complement receptor 3 (cr3, alpha m beta 2, cd11b/cd18). we show that an fha arg-gly-asp site induces enhanced b. pertussis binding to monocytes, and that this enhancement is blocked by antibodies directed against cr3. enhancement requires a monocyte signal transduction complex, composed ... | 1994 | 7931059 |
| [bacteriological and clinical studies of biapenem (l-627) in pediatrics]. | bacteriological and clinical studies in the pediatric field have been performed on biapenem (l-627), a newly-developed carbapenem antibiotic, and the following results were obtained. 1. in the pharmacokinetic study, the plasma concentration of l-627 showed dose-dependant change: cmax was 14.6 micrograms/ml and auc was 15.4 micrograms.hr/ml with the administration of 6 mg/kg, while cmax was 49.2 micrograms/ml and auc was 60.1 micrograms.hr/ml with the administration of 12 mg/kg. after the adminis ... | 1994 | 7933527 |
| interphotoreceptor retinoid-binding protein derived peptide can induce experimental autoimmune uveoretinitis in various rat strains. | experimental autoimmune uveoretinitis (eau) is an intraocular inflammatory disease model induced by retinal specific antigens such as s-antigen and interphotoreceptor retinoid-binding protein (irbp). the present study was aimed at testing the uveitogenicity of irbp and an irbp-derived peptide in various strains of rats with different rt1 (major histocompatibility complex in rats) haplotypes. immunization with irbp induced distinct eau in lew (rt1l), wkah (rt1k) w/m (rt1k), lej (rt1j), and buf (r ... | 1994 | 7851121 |
| mechanism of the antioxidant effect of bordetella pertussis extract. | in order to gain insight into the antioxidant effect of the cell extract from bordetella pertussis (strain 18-323, phase i) pancreatic antioxidant enzymes, glutathione (gsh), lipid conjugated dienes, dna strand breaks in islet cells and the in vitro rom scavenging potential of the extract were studied in 18 hr-fasted mice after administration (i.v.) of the extract (1 mg/kg body wt) 1 hr before alloxan treatment. the antioxidant enzymes activities as well as the glutathione content, which were se ... | 1994 | 7851940 |
| unexpected isolation of bordetella pertussis from a blood culture. | bordetella pertussis was isolated from a culture of blood from a 31-year-old man with wegener's granulomatosis. the organism was detected with the bactec 9240 system after 6 days of incubation and was confirmed as b. pertussis by the centers for disease control and prevention. to our knowledge, this is the first published report of the recovery of b. pertussis from blood. | 1994 | 7852585 |
| characterization of immunological activity of a low toxicity antitumor lipopolysaccharide from bordetella pertussis. | immunological properties of a low toxicity lipopolysaccharide (bp-lps) extracted from bordetella pertussis (tohama strain) which was reported to have high antitumor activity against murine tumors were examined and compared with those of lps extracted from other enterobacteria. the activation or stimulation of murine macrophages and lymphocytes by these lps, including tnf induction, was found to be similar. however, bp-lps was clearly less active in its stimulation of murine and human neutrophils ... | 1994 | 7854214 |
| enhancing effects of pertussis toxin b oligomer on the immunogenicity of influenza vaccine administered intranasally. | influenza vaccines together with pertussis toxin b oligomer (ptb) purified from a culture supernatant of bordetella pertussis were administered intranasally into mice to test for an adjuvant effect of the ptb. an inactivated virus vaccine and an ether-treated ha vaccine prepared from influenza virus a/yamagata/120/86 (h1n1) and formulated with ptb, stimulated production of serum haemagglutinin inhibition (hi) antibody and pulmonary and endotracheal secretory iga antibody to high titres. in addit ... | 1994 | 7856288 |
| translocation of a hybrid yope-adenylate cyclase from yersinia enterocolitica into hela cells. | pathogenic bacteria of the genus yersinia release in vitro a set of antihost proteins called yops. upon infection of cultured epithelial cells, extracellular yersinia pseudotuberculosis transfers yope across the host cell plasma membrane. to facilitate the study of this translocation process, we constructed a recombinant yersinia enterocolitica strain producing yope fused to a reporter enzyme. as a reporter, we selected the calmodulin-dependent adenylate cyclase of bordetella pertussis and we mo ... | 1994 | 7885236 |
| obtention of ovine ige from heterohybridoma. | mesenteric and bronchial lymph node cells from sheep immunized with ascaris suum antigens in combination with bordetella pertussis vaccine were fused with mouse myeloma cell lines, p3-x63-ag8.653, nso.u and ns1.1.ag1.1. one heterohybridoma cell line (ns1.1.ag1.1 x sheep) producing ovine immunoglobulin e was detected by the passive cutaneous anaphylaxis test. | 1994 | 7889039 |
| recombinant microbial adp-ribosylating toxins of bordetella pertussis, vibrio cholerae, and enterotoxigenic escherichia coli: structure, function, and toxoid vaccine development. | 1994 | 7764758 | |
| effect of temperature and host factors on the activities of pertussis toxin and bordetella adenylate cyclase. | pertussis toxin and adenylate cyclase toxin both contribute to the pathogenesis of whooping cough. production of these proteins is controlled by the bvg locus, which is inactive at 25 degrees c, but at 37 degrees c produces a vir+ phenotype. in view of the temperature dependence of virulence factor synthesis, the effects of temperature and host factors on their action were examined. the nad glycohydrolase activity of the s1 subunit of pertussis toxin was enhanced by chaps, a zwitterionic deterge ... | 1994 | 7803392 |
| use of cyclodextrin as an agent to induce excretion of bordetella pertussis antigens. | this paper attempts to provide an explanation for the effect of cyclodextrin on the yield of bordetella pertussis soluble antigens. it was demonstrated that the addition of cyclodextrin to the synthetic stainer-scholte liquid medium enhances the level of the intracellular form of adenylate cyclase (200 kda) in the supernate. in addition to this effect, it has been reported that cyclodextrin also enhances the levels of two other extracellular proteins, pertussis toxin and filamentous hemagglutini ... | 1994 | 7804162 |
| induction of natural killer cell migration by monocyte chemotactic protein-1, -2 and -3. | under certain physiological and pathological conditions, natural killer (nk) cells rapidly accumulate in tissues. chemokines are an essential component of the current paradigm of leukocyte recruitment. the present study was designed to investigate the responsiveness of nk cells to the prototypic c-c chemokine, monocyte chemotactic protein-1 (mcp-1). mcp-1 induced migration across filters of interleukin (il)-2-activated nk cells, whereas it was a weak attractant for unstimulated cells. maximal in ... | 1994 | 7805752 |
| chemical fatty acylation confers hemolytic and toxic activities to adenylate cyclase protoxin of bordetella pertussis. | adenylate cyclase toxin (act), a virulence factor of bordetella pertussis, acquires hemolytic and toxic activities after post-translational modification of the cyaa gene product, cyaa. the exact nature of this modification is unknown, but homology to the related repeat toxin alpha-hemolysin of escherichia coli suggests that fatty acylation of a lysine residue may be involved. in the present study, we used an in vitro chemical approach to acylate unmodified, inactive adenylate cyclase protoxin by ... | 1994 | 7806509 |
| development of pertussis serological potency test. serological assessment of antibody response induced by whole cell vaccine as an alternative to mouse protection in an intracerebral challenge model. | the current potency test for pertussis vaccines, the mouse protection test (mpt), has many disadvantages. however, no alternative is yet available. the purpose of this study is to develop a serological alternative for the mpt based on in vitro assessment of the humoral immune response against pertussis in mice. after immunization with pertussis whole cell vaccine, the mpt shows a normal primary and secondary antibody response. moreover, the i.c. challenge has a distinct booster effect on the per ... | 1994 | 7811457 |
| effects of recombinant human gamma interferon on intracellular survival of bordetella pertussis in human phagocytic cells. | several studies have demonstrated that bordetella pertussis has the ability to enter and survive intracellularly within human polymorphonuclear leukocytes (pmnl) and human monocytes/macrophages. the effects of human recombinant gamma interferon (ifn-gamma) on the survival of b. pertussis in pmnl and human monocytes, and on the oxidative burst activity of pmnl and human monocytes in response to b. pertussis were assessed in this study. ifn-gamma partially increased intracellular killing of phagoc ... | 1994 | 7812266 |
| nested pcr optimized for detection of bordetella pertussis in clinical nasopharyngeal samples. | several genes and sequences in bordetella pertussis have been used as targets in diagnostic pcr assays. a previously developed single-step pcr assay for the detection of b. pertussis was based on an insertion sequence, is480, that is present in about 70 to 80 copies in each genome. the diagnostic sensitivity, specificity, and reliability of this assay with aspirated and heat-treated samples from the nasopharynx of patients and their contacts was improved by the use of a nested pcr configuration. ... | 1994 | 7814495 |
| molecular characterization of catalase from bordetella pertussis: identification of the kata promoter in an upstream insertion sequence. | in this report we evaluate the role of catalase in the survival of bordetella pertussis within human polymorphonuclear leukocytes (pmns). crude extracts of b. pertussis exhibited a single catalase activity when subjected to non-denaturing polyacrylamide gel electrophoresis and assayed for catalase activity. a plasmid containing b. pertussis kata was identified by complementation of um255, a catalase-deficient strain of escherichia coli. the nucleotide sequence of kata predicts a 55 kda protein t ... | 1994 | 7830550 |
| [macrolides. new therapeutic prospects]. | the aim of the development of semisynthetic derivatives was to overcome the problem of chemical stability of erythromycin a in acid medium, with less variability in gastro-intestinal absorption and leading to renewed interest in macrolides. the new macrolides have the same antibacterial spectrum as erythromycin a including gram-positive and gram-negative cocci, intracellular bacteria, mycoplasma, campylobacter sp., helicobacter pylori, mycobacteria spp., gram-negative bacilli including haemophil ... | 1994 | 7831266 |
| the fnr family of transcriptional regulators. | homologues of the transcriptional regulator fnr from escherichia coli have been identified in a variety of taxonomically diverse bacterial species. despite being structurally very similar, members of the fnr family have disparate regulatory roles. those from shewanella putrefaciens, pseudomonas aeruginosa, pseudomonas stutzeri and rhodopseudomonas palustris are functionally similar to fnr in that they regulate anaerobic respiration or carbon metabolism. four rhizobial proteins (from rhizobium me ... | 1994 | 7747934 |
| expression and mutagenesis of recombinant cholera toxin a subunit. | adp-ribosylating protein exotoxins from vibrio cholerae (ct) and escherichia coli (lt-i) share two short regions of sequence similarity with bordetella pertussis toxin (pt). previous studies have indicated that substitution of arginine for lysine 7 within the first region of ct drastically decreases adp ribosyltransferase activity. we have more closely defined the role of other amino acids in this region by generating modified proteins in which arginine 7 was replaced with lysine (r7k), aspartat ... | 1994 | 7723660 |
| antibodies against some bacterial antigens in children. | the prevalence of bacterial antibodies was determined in 173 children aged 0-15 years. the prevalence of igg borrelia burgdorferi antibodies in titres > 500 in children less than 8 years of age was 6% while none of the older children had these antibodies in titres > 400. igg helicobacter pylori antibodies were detected only in children older than 6 years of age, with a prevalence of 6.5%, as were iga h. pylori antibodies, with a prevalence of 3.7%. the prevalence of high-titre igg campylobacter ... | 1994 | 7841725 |
| regulation of dth and ige responses by il-4 and ifn-gamma in immunized mice given pertussis toxin. | interferon-gamma (ifn-gamma) and interleukin-4 (il-4) are cytokines with important functions in regulating immune responses. ifn-gamma may be produced by cells responsible for delayed-type hypersensitivity (dth), whereas il-4 is essential for ige production. pertussis toxin (pt) from bordetella pertussis enhances both dth and ige responses, and causes enhancement of both ifn-gamma and il-4 secretion in immunized mice. in the present study, the effects of neutralizing monoclonal antibodies agains ... | 1994 | 7875744 |
| [isolation of bordetella pertussis from patients with pertussis-like symptoms and estimation of vaccine efficacy]. | in order to investigate the distribution of bordetella pertussis in nagoya city and estimate efficacy of the vaccine, we tried to isolate b. pertussis from patients with pertussis-like symptoms who went to the department of pediatric general hospitals in nagoya city from 1989 to 1992. b. pertussis were isolated from 43 patients among 164 patients with pertussis-like symptoms. all of these isolates were classified into 1, 3, 6 serotype. it was impossible to isolate any b. pertussis in 1992 becaus ... | 1994 | 7876665 |
| [the effect of the components of bordetella pertussis lipopolysaccharides on rauscher virus-induced leukosis in mice]. | 1994 | 7879511 | |
| [the interrelation of the composition of the nutrient media with the growth and biological properties of bordetella pertussis]. | 1994 | 7879517 | |
| [the determination of antibodies to bordetella pertussis exotoxin in donor sera and in the raw material for obtaining normal human immunoglobulin]. | 1994 | 7879557 | |
| expression of the bordetella pertussis p.69 pertactin adhesin in escherichia coli: fate of the carboxy-terminal domain. | the mature pertactin protein (p.69) of bordetella pertussis can be isolated from the bacterial cell surface as a polypeptide with an apparent molecular mass of 69,000 da as determined by sodium dodecyl sulphate gel electrophoresis. however the open reading frame of prn, the pertactin gene, encodes a polypeptide with a predicted molecular mass of 93,478 da, referred to as p.93. expression of the prn gene in escherichia coli leads to the synthesis of the full-length p.93 polypeptide, which is rapi ... | 1994 | 7881548 |
| negative staining can cause clumping of bordetella pertussis fimbriae. | the state of fimbriae type 2 (fim 2) and fimbriae type 3 (fim 3) preparations from bordetella pertussis were examined by negative stain electron microscopy. uranyl acetate induced clumping of fim 3 regardless of ph and was unsuitable as a stain for establishing the state of fimbriae. both ammonium molybdate and sodium phosphotungstate were able to show the differences in fim 3 stored at ph 7.2 and ph 9.5. | 1994 | 7881899 |
| isotype and antigen specificity of pertussis agglutinins following whole-cell pertussis vaccination and infection with bordetella pertussis. | elevated agglutinin titers have been shown to correlate with protection from disease following whole-cell pertussis vaccination, but the isotype and antigen specificity of human agglutinating antibodies is unknown. in 13 immunoassays, immunoglobulin g antifimbria antibodies had the strongest correlation with agglutinin titers following culture-proven infection with bordetella pertussis (r' = 0.79; p < 0.0001) and following whole-cell pertussis vaccination (r' = 0.87, p < 0.0001). | 1994 | 7509316 |
| structural relationship between the s1 and s4 subunits of pertussis toxin. | pertussis toxin, the most important protective antigen of bordetella pertussis, is a 106-kda hexameric protein composed of an a-protomer (subunit s1) and a pentameric b-oligomer (s2 + s3 + 2s4 + s5). the most potent mouse-protective monoclonal antibodies against both respiratory and intracerebral infections were specified for either s1 or s4 and competed with each other in binding to epitopes of native pertussis toxin captured by haptoglobin or in solution, although they did not compete on unfol ... | 1994 | 7510256 |
| localization of antigenic domains on the major subunits of bordetella pertussis serotype 2 and 3 fimbriae. | antibody-binding domains on the major subunits of bordetella pertussis serotype 2 (fim2) and 3 fimbriae (fim3) have been identified using synthetic peptides which were screened for recognition by anti-protein monoclonal antibodies (mabs). the presence of non-contiguous fimbrial epitopes was demonstrated by both anti-fim2 and anti-fim3 mabs, several of which recognized at least two peptides that were discontinuous in the amino acid sequence of the corresponding subunits. the specificity of one ma ... | 1994 | 7512870 |
| formaldehyde treatment of proteins can constrain presentation to t cells by limiting antigen processing. | proteins to be used as vaccines are frequently treated with formaldehyde, although little is known about the effects of this treatment on protein antigenicity. to investigate the effect of formaldehyde treatment on antigen recognition by t cells, we compared the in vitro t-cell response to proteins that have been formaldehyde treated with the response to untreated proteins. we found that peripheral blood mononuclear cells from individuals vaccinated with three formaldehyde-treated proteins (pert ... | 1994 | 7513307 |
| effect of antibiotics on bordetella pertussis adhering activity: hypothesis regarding mechanism of action. | microbial adherence to epithelial cell surfaces has been implicated as the first step in the initiation of several infectious diseases. the ability of antibiotics to affect the properties of bacterial adherence to cell surfaces may be a criterion in selecting antibiotics for therapy. this study was performed in order to investigate the activity of amoxicillin, chloramphenicol, and clarithromycin in modifying the adhering activity of bordetella pertussis to human epithelial cells. the actions of ... | 1994 | 7515782 |
| filamentous hemagglutinin of bordetella pertussis. a bacterial adhesin formed as a 50-nm monomeric rigid rod based on a 19-residue repeat motif rich in beta strands and turns. | the filamentous hemagglutinin (fha) of bordetella pertussis is an adhesin that binds the bacteria to cells of the respiratory epithelium in whooping-cough infections. mature fha is a 220 kda secretory protein that is highly immunogenic and has been included in acellular vaccines. we have investigated its structure by combining electron microscopy and circular dichroism spectroscopy (cd) with computational analysis of its amino acid sequence. the fha molecule is 50 nm in length and has the shape ... | 1994 | 7519681 |
| modulation of hepatic mrna levels after administration of lipopolysaccharide and diphtheria and tetanus toxoids and pertussis vaccine adsorbed (dtp vaccine) to mice. | administration of whole-cell diphtheria and tetanus toxoids and pertussis vaccine adsorbed (dtp vaccine) caused marked depression in the expression of mrna for isozymes of cytochrome p-450 in the livers of endotoxin-responsive and nonresponsive mice. the levels of expression of mrna for a polycyclic aromatic hydrocarbon-inducible (cyp1a2) and an ethanol-inducible (cyp2e1) form of p-450 were reduced by 70% to 80% 8 to 12 hr after vaccination or bordetella pertussis endotoxin administration. these ... | 1994 | 7523268 |
| adenylate cyclase toxin (cyaa) of bordetella pertussis. evidence for the formation of small ion-permeable channels and comparison with hlya of escherichia coli. | the interaction between the adenylate cyclase toxin (cyaa) of bordetella pertussis and lipid was studied using the lipid bilayer assay. the addition of cyaa to the aqueous phase bathing lipid bilayer membranes composed of different lipids resulted in the increase of the membrane conductance. this increase was rather small for membranes formed of pure lipids as compared with lipid mixtures such as asolectin. the toxin formed in asolectin membranes small transient ion-permeable channels with a sin ... | 1994 | 7525549 |
| g-proteins in alpha 1-adrenoceptor mediated prostatic smooth muscle contraction. | the role of signal transducing guanine-nucleotide binding proteins (g-proteins) in alpha 1-receptor mediated smooth muscle contractions was investigated in human hyperplastic prostatic tissue. the selective alpha 1-receptor agonist phenylephrine (pe) evoked dose dependent contractions antagonized by the alpha 1-receptor blockers prazosin (ec50 10 nm) and ym617 (ec50 3 nm). application of nifedipine (1-10,000 nm), a blocker of voltage-dependent l-type ca(2+)-channels (vdcc), inhibited the pe evok ... | 1994 | 7527606 |
| lectin domains in the toxin of bordetella pertussis: selectin mimicry linked to microbial pathogenesis. | the pathogenesis of many infectious diseases is critically determined by prokaryotic lectins which enable differential recognition and activation of targeted eukaryotic cells. some bacterial adhesins mimic and co-opt eukaryotic cell-cell adhesion motifs. this is illustrated by the toxin of bordetella pertussis. pertussis toxin mediates intoxication of eukaryotic cells by elevation of camp and it serves as an adhesin binding the bacteria to ciliated cells and respiratory macrophages. these activi ... | 1994 | 7535138 |
| elucidation of linear epitopes of pertussis toxin using overlapping synthetic decapeptides: identification of a human b-cell determinant in the s1 subunit indicative of acute infections. | to identify relevant linear epitopes within the immunodominant adp-ribosyl transferase (s1 subunit) of pertussis toxin (pt), its complete amino acid sequence was synthesized as consecutive, overlapping decapeptides on solid phase and probed for seroreactivity with pertussis specific human antisera in 'peptide scans'. comparison of the resulting antigenic profiles revealed two distinct types of human antisera, though amino acids 140-200 could not be assessed as the corresponding peptides reacted ... | 1994 | 7536288 |
| a bordetella pertussis acellular vaccine candidate: antigenic characterization and antibody induction. | a single-step chromatography on matrex-gel blue a has been employed to obtain soluble extracts containing some of the most important antigens of bordetella pertussis, pertussis toxin (pt), filamentous hemagglutinin (fha), pertactin (69-kda outer membrane protein), fimbriae (fim2 and fim3) and adenylate cyclase (ac). two supernatants, p19 (48.8 mg pt, 6.8 mg fha, 17.3 mg ac, 13 mg fim2 and 4.9 mg fim3 per liter) and p21 (0.1 mg pt, 0.07 mg fha, 0.46 mg fim2 and 0.94 mg fim3 per liter), resulting ... | 1994 | 7549983 |
| [bordetella pertussis: a case of whooping cough in a newborn]. | 1994 | 7624981 | |
| structure of a pertussis toxin-sugar complex as a model for receptor binding. | pertussis toxin is an exotoxin from the bacterium bordetella pertussis which is important the pathogenesis of whooping cough and the generation of a protective immune response. the diverse biological activities of the toxin depend on its ability to recognize carbohydrate-containing receptors on a wide variety of eukaryotic cells. we present here the crystal structure of pertussis toxin complexed with a soluble oligosaccharide from transferrin. binding sites for the terminal sialic acid-galactose ... | 1994 | 7634099 |
| effect of tracheal cytotoxin from bordetella pertussis on human neutrophil function in vitro. | the infiltration of neutrophils which phagocytose and kill microorganisms is an important defense mechanism against infections of the airways. bordetella pertussis is a human respiratory pathogen which colonizes ciliated epithelium, causing whooping cough. we have investigated the effects of the peptidoglycan fragment tracheal cytotoxin (tct) of b. pertussis on human neutrophil function in vitro. tct (10(-6) to 10(-8) m) was toxic for human neutrophils, as measured by lactate dehydrogenase relea ... | 1994 | 8300220 |
| analysis of protective and nonprotective monoclonal antibodies specific for bordetella pertussis lipooligosaccharide. | in this study, it has been determined that immunoglobulin g1 (igg1) and igg3 monoclonal antibodies directed to the lipooligosaccharide a of bordetella pertussis were able to protect mice from fatal aerosol infection. no correlation was found between the bactericidal activity in vitro in the presence of complement and the protection in mice, since a bactericidal igg3 did not elicit protection. in addition, no significant difference in protective capacity was observed with bactericidal and nonbact ... | 1994 | 8300232 |
| autophosphorylation and phosphotransfer in the bordetella pertussis bvgas signal transduction cascade. | expression of adhesins, toxins, and other virulence factors of bordetella pertussis is under control of the bvga and bvgs proteins, members of a bacterial two-component signal transduction family. bvga bears sequence similarity to regulator components, whereas bvgs shows similarity to both sensor and regulator components. bvga and the cytoplasmic portion of bvgs ('bvgs) were overexpressed and purified. 'bvgs autophosphorylated with the gamma-phosphate from [gamma-32p]atp and phosphorylated bvga. ... | 1994 | 8302847 |