Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the effect of conventional mechanical periodontal treatment on red complex microorganisms and clinical parameters in down syndrome periodontitis patients: a pilot study. | periodontal disease (pd) is induced by a complex microbiota, such as porphyromonas gingivalis, tannerella forsythia, and treponema denticola (together called the red complex), which triggers intense inflammatory reaction. down syndrome (ds) individuals demonstrate a high prevalence of pd compared with those who are otherwise chromosomally normal (euploids). this pilot study aimed to evaluate the effect of non-surgical periodontal treatment in ds chronic periodontitis patients on clinical and mic ... | 2015 | 25367612 |
| screening and development of dna aptamers specific to several oral pathogens. | aptamers are composed of single-stranded oilgonucleotides that can selectively bind desired molecules. it has been reported that rna or dna could act as not only a genetic messenger but also a catalyst in metabolic pathways. rna aptamers (average sizes 40-50 bp) are smaller than antibodies and have strong binding capacities to target molecules, similar to antigen-antibody interactions. once an aptamer was selected, it can be readily produced in large quantities at low cost. the objectives of thi ... | 2015 | 25269812 |
| electrophoresis of periodontal pathogens in poly(ethyleneoxide) solutions with uncoated capillary. | periodontitis is a prevalent inflammatory disease caused by different species of anaerobic bacteria such as porphyromonas gingivalis (p.g), treponema denticola (t.d), and tannerella forsythia (t.f). we compared the separation result of dna ladders in hydroxyethyl cellulose, poly(ethyleneoxide) (peo), and polyethylene glycol and analyzed the effect of polymer concentration, electric field, and temperature of the background electrolyte on the separation performance. results demonstrated that there ... | 2015 | 25447490 |
| oral microbiome in chinese patients with aggressive periodontitis and their family members. | to investigate the microbiome composition in chinese patients with aggressive periodontitis (agp), and to compare the similarity of bacterial profiles between agp patients and their family members. | 2015 | 26412568 |
| periodontal status and pathogenic bacteria after gastric bypass: a cohort study. | the aim this study was to evaluate the influence of gastric bypass surgery (gbs) on periodontal disease and quantify the periodontopathogenic bacteria in patients undergoing this surgery. | 2015 | 25924695 |
| β2-glycoprotein i-dependent anti-cardiolipin antibodies associated with periodontitis in patients with systemic lupus erythematosus. | it was reported that patients with systemic lupus erythematosus (sle) exhibited increased levels of anticardiolipin (anti-cl) antibodies, a class of antiphospholipid antibodies associated with thrombosis. β2-glycoprotein i (β2gpi) has been considered as the actual target antigen for anti-cl antibodies. this study investigates the association of periodontal infection with anti-cl antibodies in patients with sle. | 2015 | 25817824 |
| pilot study on the clinical and microbiological effect of subgingival glycine powder air polishing using a cannula-like jet. | to assess the efficacy of subgingival glycine powder air polishing (gpap) during supportive periodontal therapy (spt). | 2015 | 25156125 |
| functional biomarkers for chronic periodontitis and insights into the roles of prevotella nigrescens and fusobacterium nucleatum; a metatranscriptome analysis. | periodontitis is the most prevalent inflammatory disease worldwide and is caused by a dysbiotic subgingival biofilm. here we used metatranscriptomics to determine the functional shift from health to periodontitis, the response of individual species to dysbiosis and to discover biomarkers. | 2015 | 28721234 |
| polymerase chain reaction as a prospect for the early diagnosis and prediction of periodontal diseases in adolescents. | this study was to determine the markers representative of pathogenic periodontal microflora [prevotella intermedia (p.i), tannerella forsythia (t.f) [bacteroides forsythus], treponema denticola (t.d), actinobacillus actinomycetemcomitans (a.a), porphyromonas gingivalis (p.g)] in the dental plaque of adolescents with various degrees of severity of periodontium inflammation. | 2015 | 25266040 |
| periodontopathogens and human β-defensin-2 expression in gingival crevicular fluid from patients with periodontal disease in guangxi, china. | periodontal diseases are often induced by periodontopathogens, which are always exposed to certain innate immune factors in gingival crevicular fluid, including human β-defensin-2 (hbd-2). this study aims to investigate the relationship among periodontopathogens, clinical parameters and hbd-2 expression. | 2015 | 25065393 |
| effect of repeated adjunctive antimicrobial photodynamic therapy on subgingival periodontal pathogens in the treatment of chronic periodontitis. | the aim of this study was to compare the effect of subgingival ultrasonic scaling followed by repeated (three times) antimicrobial photodynamic therapy (pdt), ultrasonic scaling alone (us), and scaling and root planing with hand instruments (srp) for initial periodontal treatment. twenty-seven non-smoking systemically healthy chronic periodontitis patients were included. residual pockets ≥4 mm deep and bleeding on probing were debrided either with srp, us alone, or us followed by a single episod ... | 2015 | 25056413 |
| subgingivally applied minocycline microgranules in subjects with chronic periodontitis: a randomized clinical and microbiological trial. | the aim of this study was to evaluate clinical and microbiological effects of subgingival minocycline microgranules when used as an adjunct to scaling and root planing in subjects with chronic periodontitis. twenty-six non-smoker volunteers participated in the study. four opposite sites, clinically standardized, with bleeding on probing (bop) and pocket depth (pd) ≥ 6 mm were selected. baseline bop, pd and clinical attachment level (cal) were measured and microbiological samples were collected f ... | 2015 | 26355881 |
| the association between subgingival periodontal pathogens and systemic inflammation. | to investigate associations between periodontal disease pathogens and levels of systemic inflammation measured by c-reactive protein (crp). | 2015 | 26309048 |
| clinical and microbiologic evaluation of scaling and root planing per quadrant and one-stage full-mouth disinfection associated with azithromycin or chlorhexidine: a clinical randomized controlled trial. | conflicting data about the protocol of choice for non-surgical periodontal therapy with adjuvant use are still reported. this study aims to evaluate, through clinical and microbiologic parameters, the systemic use of azithromycin (az) and chlorhexidine (chx) as adjuvants to non-surgical periodontal treatment performed by one-stage full-mouth disinfection (fmd) within 24 hours or conventional quadrant scaling (qs) in four weekly sections. | 2015 | 26252751 |
| occurrence of periodontal pathogens in ethnic groups from a native brazilian reservation. | the present study was designed to evaluate the occurrence of periodontal pathogens in the subgingival biofilm of 100 native brazilians living at the umutina indian reservation, mato grosso state, brazil. | 2015 | 25847068 |
| antimicrobial photodynamic effect to treat residual pockets in periodontal patients: a randomized controlled clinical trial. | a randomized controlled clinical trial was designed to evaluate the efficacy of the photodynamic therapy (pdt) in the treatment of residual pockets of chronic periodontitis patients. | 2015 | 25808980 |
| a. actinomycetemcomitans profile and red complex bacterial species of an afro-brazilian community: a comparative study. | the primary aim of this cross-sectional study was to compare the levels of red complex bacteria between afro-brazilian and non afro-brazilian cohort. the secondary aim was to compare the distribution of both aggregatibacter actinomycetemcomitans serotype b and its jp2 strains among participants who harboured this bacterial species. | 2015 | 25757149 |
| inter- and intra-test agreement of three commercially available molecular diagnostic tests for the identification of periodontal pathogens. | the aim of the study was to compare the agreement and reproducibility of three test kits (iai pado test 4⋅5®, micro-ident®, and carpegen® perio diagnostik) regarding the detection of porphyromonas gingivalis, tannerella forsythia, treponema denticola, and aggregatibacter actinomycetemcomitans. | 2015 | 25680706 |
| short-term effect of removal of fixed orthodontic appliances on gingival health and subgingival microbiota: a prospective cohort study. | the aim of this prospective longitudinal study was to assess and compare the microbiological and clinical parameters of patients wearing a fixed orthodontic appliance, as opposed to 10 days after the bracket had been removed following treatment. | 2015 | 25631494 |
| periodontopathogen levels following the use of an er:yag laser in the treatment of chronic periodontitis. | inflammatory periodontal diseases are initiated by microbial biofilms. the reduction of the biofilm is important in the management of the disease. this study compares periodontopathogen levels following the treatment of chronic periodontitis using er:yag laser debridement and mechanical scaling and root planing. | 2015 | 25630495 |
| microbial leakage at the implant-abutment connection due to implant insertion maneuvers: cross-sectional study 5 years postloading in healthy patients. | the aim of this study was to test if stress on the prosthetic connection during insertion maneuvers can induce micro-warping at the implant connection. from september 2011 to july 2013, patients with implants loaded for at least 5 years that were placed with 2 different insertion implant mounters-mp (conventional) and me (mountless)-were selected from all of those who had received dental implant therapy in the past and were attending routine check-up or spontaneous visits during the study period ... | 2015 | 25536224 |
| distribution of 8 periodontal microorganisms in family members of chinese patients with aggressive periodontitis. | to date, no information on the distribution of periodontal microorganisms among family members of chinese patients with aggressive peridontitis (agp) is available. the aim of the present study was to investigate the probability of transmission of eight periodontal microorganisms between patients with aggressive periodontitis and their family members. | 2015 | 25526624 |
| construction of coa-dependent 1-butanol synthetic pathway functions under aerobic conditions in escherichia coli. | 1-butanol is an important industrial platform chemical and an advanced biofuel. while various groups have attempted to construct synthetic pathways for 1-butanol production, efforts to construct a pathway that functions under aerobic conditions have met with limited success. here, we constructed a coa-dependent 1-butanol synthetic pathway that functions under aerobic conditions in escherichia coli, by expanding the previously reported (r)-1,3-butanediol synthetic pathway. the pathway consists of ... | 2015 | 25865277 |
| periodontopathogen and epstein-barr virus-associated periapical periodontitis may be the source of retrograde infectious peri-implantitis. | herpesviral-bacterial synergism may play a role in periodontitis and peri-implantitis etiopathogenesis. periapical periodontitis (pp) lesions can predict future apical peri-implantitis complications. | 2015 | 23675952 |
| microbiological assessment of the implant-abutment interface in different connections: cross-sectional study after 5 years of functional loading. | to evaluate the bacterial microflora present inside the implant connection and in the peri-implant sulcus fluid of healthy implants, and to analyze the relationships between these harboring sites for four different implant systems after at least 5 years of functional loading. | 2015 | 24665919 |
| peri-implant response and microflora in organ transplant patients 1 year after prosthetic loading: a prospective controlled study. | a recent study conducted in humans demonstrated for the first time that bone loss in the immediate period after implant insertion before loading did not significantly differ in organ transplant recipients with respect to normal subjects. | 2015 | 24612063 |
| subgingival plaque sampling after combined mechanical and antibiotic nonsurgical periodontal therapy. | this study aimed to make a comparison of two sampling strategies of subgingival plaque after combined mechanical-antibiotic periodontal therapy. | 2015 | 24554003 |
| internal bacterial colonization of implants: association with peri-implant bone loss. | the aim of the present longitudinal study was to investigate bacterial colonization of the internal implant cavity and to evaluate a possible association with peri-implant bone loss. | 2015 | 24861845 |
| relationship between expression of human gingival beta-defensins and levels of periodontopathogens in subgingival plaque. | human beta-defensins (hbds) are a group of antimicrobial peptides important in epithelial innate immunity, and their differential expression is associated with periodontal diseases. the aim of this study was to explore relationships among hbds, total subgingival bacteria and periodontopathogens in healthy subjects and in patients with chronic periodontitis. | 2015 | 24814979 |
| assessment of periodontal and opportunistic flora in patients with peri-implantitis. | to assess the presence of periodontal and opportunistic organisms in patients with peri-implantitis. | 2015 | 24720498 |
| occurrence of red complex microorganisms and aggregatibacter actinomycetemcomitans in patients with diabetes. | the aim of the present study was to analyze the occurrence of porphyromonas gingivalis (p. gingivalis), tannerella forsythia (t. forsythia), treponema denticola (t. denticola), and aggregatibacter actinomycetemcomitans (a. actinomycetemcomitans) in patients with diabetes. | 2015 | 23857867 |
| quantitative analysis of classical and new putative periodontal pathogens in subgingival biofilm: a case-control study. | a number of species/phylotypes have been newly implicated as putative periopathogens. the objective of this study was to explore associations among classical and new pathogens in subgingival biofilm and to assess their relative importance to chronic periodontitis. | 2015 | 25040261 |
| antimicrobial effectiveness of cetylpyridinium chloride and zinc chloride-containing mouthrinses on bacteria of halitosis and peri-implant disease. | to clarify the antimicrobial efficacy of zinc chloride (zncl2) and cetylpyridinium chloride (cpc) by testing their impact on the growth of seven bacterial strains known to be involved in the pathophysiology of both peri-implant disease and halitosis-staphylococcus aureus, streptococcus mutans, porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, treponema denticola, and tannerella forsythia. | 2015 | 26478974 |
| microbial diversity of the supra- and subgingival biofilm of healthy individuals after brushing with chlorhexidine- or silver-coated toothbrush bristles. | nanoparticulate silver has recently been reported as an effective antimicrobial agent. the aim of this clinical study was to investigate the potential changes on the oral microbiota of healthy individuals after controlled brushing with chlorhexidine- or silver-coated toothbrush bristles. twenty-four healthy participants were enrolled in this investigation and randomly submitted to 3 interventions. all the participants received, in a crossover format, the following toothbrushing interventions: (i ... | 2015 | 25581046 |
| active invasion of porphyromonas gingivalis and infection-induced complement activation in apoe-/- mice brains. | periodontal disease is a polymicrobial inflammatory disease that leads to chronic systemic inflammation and direct infiltration of bacteria/bacterial components, which may contribute to the development of alzheimer's disease. apoe-/- mice were orally infected (n = 12) with porphyromonas gingivalis, treponema denticola, tannerella forsythia, and fusobacterium nucleatum as mono- and polymicrobial infections. apoe-/- mice were sacrificed following 12 and 24 weeks of chronic infection. bacterial gen ... | 2015 | 25061055 |
| oral cavity anaerobic pathogens in biofilm formation on voice prostheses. | a polymerase chain reaction (pcr)-based method has been used to identify oral anaerobic pathogens in biofilms on voice prostheses. the purpose of the present study was to determine the location of those pathogens inside the biofilms. | 2015 | 24677258 |
| association of treponeme species with atypical foot lesions in goats. | five uk goat farms with high levels of lameness (prevalence 14-67 per cent) were investigated. on two farms (farms 1 and 2), the animals presented with typical footrot lesions. the remaining three farms (farms 3, 4 and 5) presented with infected lesions on the foot that did not resemble footrot. these lesions were observed to start from the white line or sole but the interdigital space was rarely affected. swabs were processed by pcr to assess the presence of dichelobacter nodosus and three spec ... | 2015 | 25977404 |
| epstein-barr virus associated peri-implantitis: a split-mouth study. | herpesviral-bacterial synergism may play a potential role in periodontitis and peri-implantitis (pi) etiopathogenesis. pi lesions can worsen depending on specific microbial challenge and host susceptibility. this cross-sectional split-mouth study aimed to substantiate herpesviral-bacterial co-infection in pi patients and assess associations with periodontopathogen salivary contamination. | 2015 | 24802631 |
| expression of alzheimer-type neurofibrillary epitopes in primary rat cortical neurons following infection with enterococcus faecalis. | the neurofibrillary tau pathology and amyloid deposits seen in alzheimer's disease (ad) also have been seen in bacteria-infected brains. however, few studies have examined the role of these bacteria in the generation of tau pathology. one suggested link between infection and ad is edentulism, the complete loss of teeth. edentulism can result from chronic periodontal disease due to infection by enterococcus faecalis. the current study assessed the ability to generate early alzheimer-like neurofib ... | 2015 | 26834627 |
| expression of alzheimer-type neurofibrillary epitopes in primary rat cortical neurons following infection with enterococcus faecalis. | the neurofibrillary tau pathology and amyloid deposits seen in alzheimer's disease (ad) also have been seen in bacteria-infected brains. however, few studies have examined the role of these bacteria in the generation of tau pathology. one suggested link between infection and ad is edentulism, the complete loss of teeth. edentulism can result from chronic periodontal disease due to infection by enterococcus faecalis. the current study assessed the ability to generate early alzheimer-like neurofib ... | 2015 | 26834627 |
| intestinal spirochaetes (genus brachyspira) colonise wild birds in the southern atlantic region and antarctica. | the genus brachyspira contains well-known enteric pathogens of veterinary significance, suggested agents of colonic disease in humans, and one potentially zoonotic agent. there are recent studies showing that brachyspira are more widespread in the wildlife community than previously thought. there are no records of this genus in wildlife from the southern atlantic region and antarctica. our aim was therefore, to determine whether intestinal spirochaetes of genus brachyspira colonise marine and co ... | 2015 | 26584828 |
| spirochetal motility and chemotaxis in the natural enzootic cycle and development of lyme disease. | two-thirds of all bacterial genomes sequenced to-date possess an organelle for locomotion, referred to as flagella, periplasmic flagella or type iv pili. these genomes may also contain a chemotaxis-signaling system which governs flagellar rotation, thus leading a coordinated function for motility. motility and chemotaxis are often crucial for infection or disease process caused by pathogenic bacteria. although motility-associated genes are well-characterized in some organisms, the highly orchest ... | 2015 | 26519910 |
| flagellar motility of the pathogenic spirochetes. | bacterial pathogens are often classified by their toxicity and invasiveness. the invasiveness of a given bacterium is determined by how capable the bacterium is at invading a broad range of tissues in its host. of mammalian pathogens, some of the most invasive come from a group of bacteria known as the spirochetes, which cause diseases, such as syphilis, lyme disease, relapsing fever and leptospirosis. most of the spirochetes are characterized by their distinct shapes and unique motility. they a ... | 2015 | 26481969 |
| brachyspira suanatina sp. nov., an enteropathogenic intestinal spirochaete isolated from pigs and mallards: genomic and phenotypic characteristics. | the genus brachyspira currently encompasses seven valid species that colonize the intestines of mammals and birds. in a previous study a group of strongly haemolytic isolates from pigs and mallards was provisionally described as a new species within genus brachyspira, "b. suanatina", and enteropathogenic properties were demonstrated in a porcine challenge model. | 2015 | 26458507 |
| can oral infection be a risk factor for alzheimer's disease? | alzheimer's disease (ad) is a scourge of longevity that will drain enormous resources from public health budgets in the future. currently, there is no diagnostic biomarker and/or treatment for this most common form of dementia in humans. ad can be of early familial-onset or sporadic with a late-onset. apart from the two main hallmarks, amyloid-beta and neurofibrillary tangles, inflammation is a characteristic feature of ad neuropathology. inflammation may be caused by a local central nervous sys ... | 2015 | 26385886 |
| individuality, phenotypic differentiation, dormancy and 'persistence' in culturable bacterial systems: commonalities shared by environmental, laboratory, and clinical microbiology. | for bacteria, replication mainly involves growth by binary fission. however, in a very great many natural environments there are examples of phenotypically dormant, non-growing cells that do not replicate immediately and that are phenotypically 'nonculturable' on media that normally admit their growth. they thereby evade detection by conventional culture-based methods. such dormant cells may also be observed in laboratory cultures and in clinical microbiology. they are usually more tolerant to s ... | 2015 | 26629334 |
| digital dermatitis in dairy cows: a review of risk factors and potential sources of between-animal variation in susceptibility. | digital dermatitis (dd) is a bacterial disease that primarily affects the skin on the heels of cattle. it is a major cause of lameness in dairy cows and a significant problem for the dairy industry in many countries, causing reduced animal welfare and economic loss. a wide range of infection levels has been found on infected farms, prompting investigations into both farm level and animal level risk factors for dd occurrence. there also appears to be individual variation between animals in suscep ... | 2015 | 26479371 |
| association between periodontitis and alzheimer's disease. | alzheimer's disease (ad) is a neurodegenerative disease which significantly increases with age. its onset can be either early or late. ad is characterized by the salient inflammatory features, microglial activation, and increased levels of proinflammatory cytokines which contribute to the inflammatory status of the central nervous system (cns). whereas, periodontitis is a common oral infection associated with the gram negative anaerobic bacteria. periodontitis can be marked as a "low-grade syste ... | 2015 | 26199919 |
| complement system part ii: role in immunity. | the complement system has been considered for a long time as a simple lytic cascade, aimed to kill bacteria infecting the host organism. nowadays, this vision has changed and it is well accepted that complement is a complex innate immune surveillance system, playing a key role in host homeostasis, inflammation, and in the defense against pathogens. this review discusses recent advances in the understanding of the role of complement in physiology and pathology. it starts with a description of com ... | 2015 | 26074922 |
| porphyromonas gingivalis periodontal infection and its putative links with alzheimer's disease. | periodontal disease (pd) and alzheimer's disease (ad) are inflammatory conditions affecting the global adult population. in the pathogenesis of pd, subgingival complex bacterial biofilm induces inflammation that leads to connective tissue degradation and alveolar bone resorption around the teeth. in health, junctional epithelium seals the gingiva to the tooth enamel, thus preventing bacteria from entering the gingivae. chronic pd involves major pathogens (porphyromonas gingivalis, treponema dent ... | 2015 | 26063967 |
| intracellular concentrations of borrelia burgdorferi cyclic di-amp are not changed by altered expression of the cdaa synthase. | the second messenger nucleotide cyclic diadenylate monophosphate (c-di-amp) has been identified in several species of gram positive bacteria and chlamydia trachomatis. this molecule has been associated with bacterial cell division, cell wall biosynthesis and phosphate metabolism, and with induction of type i interferon responses by host cells. we demonstrate that b. burgdorferi produces a c-di-amp synthase, which we designated cdaa. both cdaa and c-di-amp levels are very low in cultured b. burgd ... | 2015 | 25906393 |
| exploring the association between morgellons disease and lyme disease: identification of borrelia burgdorferi in morgellons disease patients. | morgellons disease (md) is a complex skin disorder characterized by ulcerating lesions that have protruding or embedded filaments. many clinicians refer to this condition as delusional parasitosis or delusional infestation and consider the filaments to be introduced textile fibers. in contrast, recent studies indicate that md is a true somatic illness associated with tickborne infection, that the filaments are keratin and collagen in composition and that they result from proliferation and activa ... | 2015 | 25879673 |
| current and past strategies for bacterial culture in clinical microbiology. | a pure bacterial culture remains essential for the study of its virulence, its antibiotic susceptibility, and its genome sequence in order to facilitate the understanding and treatment of caused diseases. the first culture conditions empirically varied incubation time, nutrients, atmosphere, and temperature; culture was then gradually abandoned in favor of molecular methods. the rebirth of culture in clinical microbiology was prompted by microbiologists specializing in intracellular bacteria. th ... | 2015 | 25567228 |
| oral bacteria in placental tissues: increased molecular detection in pregnant periodontitis patients. | the objective of this study was to identify the dna of oral bacteria in placental samples from women with and without periodontitis who had or had not had preterm births and/or low birthweight (pb/lbw) neonates. | 2015 | 26259070 |
| periodontal therapy effects on nitrite related to oral bacteria: a 6-month randomized clinical trial. | nitrite is a biologic factor relevant to oral and systemic homeostasis. through an oral bacteria reduction process, it was suggested that periodontal therapy and chlorhexidine (chx) rinse could affect nitrite levels, leading to negative effects, such as an increase in blood pressure. this 6-month randomized clinical trial evaluated the effects of periodontal therapeutic protocols on salivary nitrite and its relation to subgingival bacteria. | 2015 | 25811847 |
| mesaconase/fumarase fumd in escherichia coli o157:h7 and promiscuity of escherichia coli class i fumarases fuma and fumb. | mesaconase catalyzes the hydration of mesaconate (methylfumarate) to (s)-citramalate. the enzyme participates in the methylaspartate pathway of glutamate fermentation as well as in the metabolism of various c5-dicarboxylic acids such as mesaconate or l-threo-β-methylmalate. we have recently shown that burkholderia xenovorans uses a promiscuous class i fumarase to catalyze this reaction in the course of mesaconate utilization. here we show that classical escherichia coli class i fumarases a and b ... | 2015 | 26658641 |
| the treponema denticola fhbb protein is a dominant early antigen that elicits fhbb variant-specific antibodies that block factor h binding and cleavage by dentilisin. | the treponema denticola fhbb protein contributes to immune evasion by binding factor h (fh). cleavage of fh by the t. denticola protease, dentilisin, may contribute to the local immune dysregulation that is characteristic of periodontal disease (pd). although three fhbb phyletic types have been defined (fhbb1, fhbb2, and fhbb3), the in vivo expression patterns and antigenic heterogeneity of fhbb have not been assessed. here, we demonstrate that fhbb is a dominant early antigen that elicits fhbb ... | 2016 | 27113359 |
| microbial dynamics during conversion from supragingival to subgingival biofilms in an in vitro model. | the development of dental caries and periodontal diseases result from distinct shifts in the microbiota of the tooth-associated biofilm. this in vitro study aimed to investigate changes in biofilm composition and structure, during the shift from a 'supragingival' aerobic profile to a 'subgingival' anaerobic profile. biofilms consisting of actinomyces oris, candida albicans, fusobacterium nucleatum, streptococcus oralis, streptococcus mutans and veillonella dispar were aerobically grown in saliva ... | 2016 | 26033167 |
| arthritis-induced alveolar bone loss is associated with changes in the composition of oral microbiota. | rheumatoid arthritis (ra) and periodontitis (pd) are chronic inflammatory disorders that cause bone loss. pd tends to be more prevalent and severe in ra patients. previous experimental studies demonstrated that ra triggers alveolar bone loss similarly to pd. the aim of this study was to investigate if arthritis-induced alveolar bone loss is associated with modification in the oral microbiota. checkerboard dna-dna hybridization was employed to analyze forty oral bacterial species in 3 groups of c ... | 2016 | 26996070 |
| influence of highly-active antiretroviral therapy on the subgingival biofilm in hiv-infected patients. | highly-active antiretroviral therapy (haart) has been associated with alterations in subgingival biofilm and periodontal disease. the purpose of the present study was to investigate the association between different haart regimens and the prevalence of periodontal pathogens in hiv-infected patients with chronic periodontitis. | 2016 | 25283543 |
| pg1058 is a novel multidomain protein component of the bacterial type ix secretion system. | porphyromonas gingivalis utilises the bacteroidetes-specific type ix secretion system (t9ss) to export proteins across the outer membrane (om), including virulence factors such as the gingipains. the secreted proteins have a conserved carboxy-terminal domain essential for type ix secretion that is cleaved upon export. in p. gingivalis the t9ss substrates undergo glycosylation with anionic lipopolysaccharide (a-lps) and are attached to the om. in this study, comparative analyses of 24 bacteroidet ... | 2016 | 27711252 |
| a novel approach to probe host-pathogen interactions of bovine digital dermatitis, a model of a complex polymicrobial infection. | polymicrobial infections represent a great challenge for the clarification of disease etiology and the development of comprehensive diagnostic or therapeutic tools, particularly for fastidious and difficult-to-cultivate bacteria. using bovine digital dermatitis (dd) as a disease model, we introduce a novel strategy to study the pathogenesis of complex infections. | 2016 | 27908274 |
| lyme disease spirochaete borrelia burgdorferi does not require thiamin. | thiamin pyrophosphate (thdp), the active form of thiamin (vitamin b1), is believed to be an essential cofactor for all living organisms(1,2). here, we report the unprecedented result that thiamin is dispensable for the growth of the lyme disease pathogen borrelia burgdorferi (bb)(3). bb lacks genes for thiamin biosynthesis and transport as well as known thdp-dependent enzymes(4), and we were unable to detect thiamin or its derivatives in bb cells. we showed that eliminating thiamin in vitro and ... | 2016 | 27869793 |
| morgellons disease: a filamentous borrelial dermatitis. | morgellons disease (md) is a dermopathy characterized by multicolored filaments that lie under, are embedded in, or project from skin. although md was initially considered to be a delusional disorder, recent studies have demonstrated that the dermopathy is associated with tickborne infection, that the filaments are composed of keratin and collagen, and that they result from proliferation of keratinocytes and fibroblasts in epithelial tissue. culture, histopathological and molecular evidence of s ... | 2016 | 27789971 |
| motility and ultrastructure of spirochaeta thermophila. | we analyze here for the first time the swimming behavior of a thermophilic, strictly anaerobic spirochete, namely spirochaeta thermophila using high temperature light microscopy. our data show that s. thermophila very rapidly can change its morphology during swimming, resulting in cells appearing nearly linear, in cells possessing three different spiral forms, and in cells being linear at one end and spiral at the other end. in addition cells can rapidly bend by up to 180°, with their ends comin ... | 2016 | 27790206 |
| cloning, purification and characterization of the collagenase cola expressed by bacillus cereus atcc 14579. | bacterial collagenases differ considerably in their structure and functions. the collagenases colh and colg from clostridium histolyticum and cola expressed by clostridium perfringens are well-characterized collagenases that cleave triple-helical collagen, which were therefore termed as ´true´ collagenases. cola from bacillus cereus (b. cereus) has been added to the collection of true collagenases. however, the molecular characteristics of b. cereus cola are less understood. in this study, we id ... | 2016 | 27588686 |
| two different virulence-related regulatory pathways in borrelia burgdorferi are directly affected by osmotic fluxes in the blood meal of feeding ixodes ticks. | lyme disease, caused by borrelia burgdorferi, is a vector-borne illness that requires the bacteria to adapt to distinctly different environments in its tick vector and various mammalian hosts. effective colonization (acquisition phase) of a tick requires the bacteria to adapt to tick midgut physiology. successful transmission (transmission phase) to a mammal requires the bacteria to sense and respond to the midgut environmental cues and up-regulate key virulence factors before transmission to a ... | 2016 | 27525653 |
| diurnal dynamic behavior of microglia in response to infected bacteria through the udp-p2y6 receptor system. | it has long been believed that microglia morphologically transform into the activated state by retracting their long processes and consuming pathogens when bacteria infect into the brain parenchyma. in the present study, however, we showed for the first time that murine cortical microglia extend their processes towards focally injected porphyromonas gingivalis. this p. gingivalis-induced microglial process extension was significantly increased during the light (sleeping) phase than the dark (wak ... | 2016 | 27445174 |
| multilocus sequence typing of pathogenic treponemes isolated from cloven-hoofed animals and comparison to treponemes isolated from humans. | treponema species are implicated in many diseases of humans and animals. digital dermatitis (dd) treponemes are reported to cause severe lesions in cattle, sheep, pigs, goats, and wild elk, causing substantial global animal welfare issues and economic losses. the fastidiousness of these spirochetes has previously precluded studies investigating within-phylogroup genetic diversity. an archive of treponemes that we isolated enabled multilocus sequence typing to quantify the diversity and populatio ... | 2016 | 27208135 |
| evidence of in vivo existence of borrelia biofilm in borrelial lymphocytomas. | lyme borreliosis, caused by the spirochete borrelia burgdorferi sensu lato, has grown into a major public health problem. we recently identified a novel morphological form of b. burgdorferi, called biofilm, a structure that is well known to be highly resistant to antibiotics. however, there is no evidence of the existence of borrelia biofilm in vivo; therefore, the main goal of this study was to determine the presence of borrelia biofilm in infected human skin tissues. archived skin biopsy tissu ... | 2016 | 27141311 |
| cyclic di-gmp regulates multiple cellular functions in the symbiotic alphaproteobacterium sinorhizobium meliloti. | sinorhizobium meliloti undergoes major lifestyle changes between planktonic states, biofilm formation, and symbiosis with leguminous plant hosts. in many bacteria, the second messenger 3',5'-cyclic di-gmp (c-di-gmp, or cdg) promotes a sessile lifestyle by regulating a plethora of processes involved in biofilm formation, including motility and biosynthesis of exopolysaccharides (eps). here, we systematically investigated the role of cdg in s. meliloti rm2011 encoding 22 proteins putatively associ ... | 2016 | 26574513 |
| bacteria in the apical root canals of teeth with apical periodontitis. | bacteria in the tooth root canal may cause apical periodontitis. this study examined the bacterial species present in the apical root canal of teeth with apical periodontitis. antibiotic sensitivity tests were performed to evaluate whether these identified bacterial species were susceptible to specific kinds of antibiotics. | 2016 | 27745799 |
| influence of the periodontal status on the initial-biofilm formation on titanium surfaces. | dental implants will be exposed to a complex ecosystem once they are placed in the oral cavity. the bacterial colonization and biofilm formation on these devices will depend not only on the physicochemical surface implant properties but also on the periodontal health conditions of the patients, as these devices are exposed. | 2016 | 24995517 |
| the current weight of evidence of the microbiologic profile associated with peri-implantitis: a systematic review. | there is currently no consensus regarding microorganisms that may be considered true peri-implant pathogens. therefore, the aim of this systematic review is to determine the weight of evidence for microorganisms related to peri-implantitis based on results of association studies. | 2016 | 27420109 |
| microbiota at teeth and implants in partially edentulous patients. a 10-year retrospective study. | to determine the microbiota at implants and adjacent teeth 10 years after placement of implants with a sandblasted and acid-etched surface. | 2016 | 25827437 |
| spirochaete flagella hook proteins self-catalyse a lysinoalanine covalent crosslink for motility. | spirochaetes are bacteria responsible for several serious diseases, including lyme disease (borrelia burgdorferi), syphilis (treponema pallidum) and leptospirosis (leptospira interrogans), and contribute to periodontal diseases (treponema denticola)(1). these spirochaetes employ an unusual form of flagella-based motility necessary for pathogenicity; indeed, spirochaete flagella (periplasmic flagella) reside and rotate within the periplasmic space(2-11). the universal joint or hook that links the ... | 2016 | 27670115 |
| interaction of spirochetes with the host fibrinolytic system and potential roles in pathogenesis. | the pathogenic spirochetes borrelia burgdorferi, b. hermsii, b. recurrentis, treponema denticola and leptospira spp. are the etiologic agents of lyme disease, relapsing fever, periodontitis and leptospirosis, respectively. lyme borreliosis is a multi-systemic disorder and the most prevalent tick-borne disease in the northern hemisphere. tick-borne relapsing fever is persistent in endemic areas worldwide, representing a significant burden in some african regions. periodontal disease, a chronic in ... | 2016 | 25914944 |
| immunologic characteristics of human gingival fibroblasts in response to oral bacteria. | there is ample evidence that gingival fibroblasts (gfs) participate in the immune response to oral bacteria and serve as immune-regulatory cells. the objective of this study was to investigate the innate immune response of gfs to oral bacteria. | 2016 | 27558278 |
| bacteria prevalence in a large italian population sample: a clinical and microbiological study. | the present study detects those bacterial species which are more strongly related to bleeding on probing, suppuration and smoking in periodontal-affected patients. nine hundred and fifty-one patients with periodontal diseases were admitted to the department of periodontology and implantology, dental school of bologna university where they underwent microbiological tests for six periodontal pathogens (actinomyces actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, treponema de ... | 2016 | 27469569 |
| impact of implant-abutment connection on osteoimmunological and microbiological parameters in short implants: a randomized controlled clinical trial. | the study aimed to determine the levels of soluble receptor activator of nuclear factor-кb ligand (srankl) and osteoprotegerin (opg) as well as their relative calculated ratio in peri-implant crevicular fluid (picf) obtained around two different types of implant-abutment connection on short implants following a 12-month monitoring period. moreover, the levels of a number of oral bacterial species were investigated in the corresponding submucosal biofilm samples. | 2016 | 27469171 |
| evaluation of efficacy of photodynamic therapy as an adjunct to nonsurgical periodontal therapy in treatment of chronic periodontitis patients: a clinico-microbiological study. | photodynamic therapy (pdt) is a local noninvasive treatment modality without side effects caused by antibiotics. the aim of this study was to evaluate the efficacy of adjunctive use of pdt with scaling and root planing as compared with srp alone in the treatment of chronic periodontitis. | 2016 | 27966504 |
| impact of heavy smoking on the clinical, microbiological and immunological parameters of patients with dental implants: a prospective cross-sectional study. | the aim of the present study was to investigate how heavy smoking influences the clinical, microbiological, and host-response characteristics in peri-implant sulcus fluid of patients with healthy dental implants. | 2016 | 26171870 |
| rapid identification and quantitation for oral bacteria based on short-end capillary electrophoresis. | high-speed capillary electrophoresis (hsce) is a promising technology applied in ultra-rapid and high-performance analysis of biomolecules (such as nucleic acids, protein). in present study, the short-end capillary electrophoresis coupled with one novel space domain internal standard method (sdis) was employed for the rapid and simultaneous analysis of specific genes from three oral bacteria (porphyromonas gingivalis (p.g), treponema denticola (t.d) and tannerela forsythia (t.f)). the reliabilit ... | 2016 | 27591633 |
| periodontitis contributes to adipose tissue inflammation through the nf-<kappa>b, jnk and erk pathways to promote insulin resistance in a rat model. | this study aimed to investigate the mechanism by which periodontitis affects the inflammatory response and systemic insulin resistance in the white adipose and liver tissues in an obese rat model. the obese model was generated by feeding rats a high fat diet. the periodontitis model was induced by ligatures and injection of "red complex", which consisted of porphyromonas gingivalis, treponema denticola, and tannerella forsythia, for two weeks. when compared with rats without periodontitis, fasti ... | 2016 | 27565999 |
| periodontal and peri-implant microbiota in patients with healthy and inflamed periodontal and peri-implant tissues. | to compare the prevalence and levels of six bacterial pathogens within the subgingival/submucosal microbiota at teeth versus implants with various clinical conditions. | 2016 | 25399962 |
| generalized aggressive periodontitis: microbiological composition and clinical parameters in non-surgical therapy. | the aim of this study was to determine the variations in periodontal parameters and microbiological composition in periodontal pockets at the baseline and 3 and 6 months post treatmentin patients with generalized aggressive periodontitis(gap) undergoing non surgical periodontal treatment combined with chlorhexidine and systemic antibiotics. medical and dental history was taken from 10 subjects, average age 30.6±2.7 years, diagnosed with gap. a non surgical periodontal treatment combined with 0.1 ... | 2016 | 28383606 |
| prevalence of periodontal pathogens as predictor of the evolution of periodontal status. | the aim of this study was to determine the relationship between the prevalence of porphyromonas gingivalis, its fima genotypes, aggregatibacter actinomycetemcomitans, tannerella forsythia, and treponema denticola and the evolution of periodontal health. in a longitudinal prospective study, samples of subgingival plaque were taken from 114 patients (37 with chronic periodontitis, 17 with gingivitis, and 60 periodontally healthy) in the course of a full periodontal examination. pcr was employed to ... | 2016 | 27888369 |
| association of porphyromonas gingivalis with high levels of stress-induced hormone cortisol in chronic periodontitis patients. | the aim of the present study was to evaluate the association between the occurrence of periodontopathogens with cortisol levels in chronic periodontitis patients. | 2016 | 26194628 |
| the distribution of porphyromonas gingivalis, tannerella forsythia, treponema denticola and aggregatibacter actinomycetemcomitans in patients with alcoholic disease: a pilot study. | both drinking and periodontal disease are serious health and social problems. findings on the effect of alcohol consumption on periodontal disease are inconclusive. | 2016 | 27627556 |
| herpesviral-bacterial co-infection in mandibular third molar pericoronitis. | the aim of this study was to assess the presence of herpesviruses and periodontopathic bacteria and to establish their potential association with pericoronitis. | 2016 | 27620215 |
| effect of helicobacter pylori infection on chronic periodontitis by the change of microecology and inflammation. | helicobacter pylori (h. pylori), a pathogen inducing peptic disease, is recently found to be binding to the progress of periodontitis. most previous studies are case-controlled, and they investigate the risk of h. pylori infection in disease the development of while few studies evaluate the correlation between h. pylori and periodontal pathogens. therefore, we investigated the correlation between h. pylori infection with periodontal parameters, periodontal pathogens and inflammation. the results ... | 2016 | 27602578 |
| longitudinal study on clinical and microbial analysis of periodontal status in pregnancy. | this study was aimed to provide a longitudinal overview of the subgingival bacterial microbiome using fluorescence in situ hybridization (fish) technique, in women in the second trimester of pregnancy (between 14 and 24 weeks), and 48 h and 8 weeks postpartum. of 31 women evaluated during pregnancy, 24 returned for the 48-h and 18 for their 8-week exams postpartum. probing depth (pd), bleeding on probing, clinical attachment level, and presence of calculus were recorded. subgingival plaque sampl ... | 2016 | 27556678 |
| bacterial load of periodontal pathogens among italian patients with chronic periodontitis: a comparative study of three different areas. | the aim of the present study was to evaluate the mean bacterial load of some periodontal pathogenic bacteria in italian patients affected by chronic periodontitis. the sample consisted of 1,762 patients with a clinical diagnosis of chronic periodontitis based on the criteria of the american academy of periodontology sampled in the period 2013-2015; 1,323 patients were from northern italy, 317 from central italy and 122 from southern italy. samples for microbiological analysis were collected from ... | 2016 | 27469562 |
| evaluation of the efficacy of a new oral gel containing carvacrol and thymol for home oral care in the management of chronic periodontitis using pcr analysis: a microbiological pilot study. | the use of chemical devices for domestic oral hygiene in periodontal patients has led to new treatment strategies aiming primarily at a control of infection. over the last few years, carvacrol and thymol (ct) have been subjected to many scientific and medical studies. the purpose of the present study was to assess the effect of ct on the red complex bacteria using polymerase chain reaction (pcr) for microbiological analysis. five patients with a diagnosis of chronic periodontitis in the age grou ... | 2016 | 27469559 |
| [study of mutual dependence of periodontal and colonic microbiome in health and pathology using nsg-sequencing]. | by using ngs-sequencing libraries of dna from periodontal swabs with primers specific to v6 region of 16s rdna prevalence of bacterial genera and species in periodontal and colonic microbiota of patients with periodontitis of different severity and healthy donors was analyzed. hyper-colonization of the colon with akkermansia muciniphila was found to be the most important maker of negative predisposition to periodontitis (t=133,7 at р=10(-6)). this result is in a good agreement with communication ... | 2016 | 27239990 |
| [use of the real-time pcr for study of the periodontal microbiome in patients with combined pathology of gastroduodenal zone and chronic periodontitis]. | the total of 54 patients with chronic periodontitis of different severity was tested using real-time pcr (dentoflor kit). the group included 38 patients with chronic gastritis. for the first time, a higher prevalence of treponema denticola in periodontium of males in comparison with females was demonstrated. the patients with chronic gastritis had more human genome dna at their periodontium than healthy individuals. non-parametric statistical analysis demonstrated high association of periodontiu ... | 2016 | 27183718 |
| detection of five potentially periodontal pathogenic bacteria in peri-implant disease: a comparison of pcr and real-time pcr. | the aim of this study was to compare the microbial analysis methods of polymerase chain reaction (pcr) and real-time pcr (rt-pcr) in terms of detection of five selected potentially periodontal pathogenic bacteria in peri-implant disease. therefore 45 samples of healthy, mucositis and peri-implantitis (n = 15 each) were assessed according to presence of the following bacteria using pcr (dna-strip technology) and rt-pcr (fluorescent dye sybr green-system): aggregatibacter actinomycetemcomitans (aa ... | 2016 | 27142589 |
| quantitative pcr studies of aggregatibacter actinomycetemcomitans and treponema denticola/tanerella forsythensis complex as etiological factors of chronic periodontitis. | real-time quantitative pcr (dentofl or kit) was used to detect dna of periodontal pathogens in specimens from 92 patients with chronic periodontitis and from a control sample of 12 normal subjects. a bimodal distribution of patients by periodontium colonization with a. actinomycetemcomitans, p. gingivalis, p. intermedia, t. forsythensis, and t. denticola was demonstrated. a new approach to interpretation of the results of quantitative evaluation of periodontal pathogens, including the notion of ... | 2016 | 26899837 |
| microbiological distribution of six periodontal pathogens between untreated italian and dutch periodontal patients. | to compare prevalence and microbial load of six periodontal pathogens between italian and dutch patients affected by chronic periodontitis, using oligonucleotide probe technology. | 2016 | 26870849 |
| antibacterial activity of curcumin against periodontopathic bacteria. | curcumin is a polyphenol extracted from root of turmeric and known to possess multifunctional properties, including antibacterial activity. although previous studies have investigated the effects of curcumin on microorganisms, available knowledge on the effects of curcumin on periodontopathic bacteria is still limited. in this study, the antibacterial effect of curcumin on periodontopathic bacteria is investigated, particularly porphyromonas gingivalis. | 2016 | 26447754 |