Publications

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puumala hantavirus excretion kinetics in bank voles (myodes glareolus).puumala hantavirus is present in bank voles (myodes glareolus) and is believed to be spread mainly by contaminated excretions. in this study, we subcutaneously inoculated 10 bank voles with puumala virus and sampled excretions until day 133 postinfection. levels of shed viral rna peaked within 11-28, 14-21, and 11-28 days postinfection for saliva, urine, and feces, respectively. the latest detection of viral rna was 84, 44, and 44 days postinfection in saliva, urine, and feces, respectively. in ...200818680643
a protein that replaces the entire cellular eif4f complex.the eif4f cap-binding complex mediates the initiation of cellular mrna translation. eif4f is composed of eif4e, which binds to the mrna cap, eif4g, which indirectly links the mrna cap with the 43s pre-initiation complex, and eif4a, which is a helicase necessary for initiation. viral nucleocapsid proteins (n) function in both genome replication and rna encapsidation. surprisingly, we find that hantavirus n has multiple intrinsic activities that mimic and substitute for each of the three peptides ...200818971945
genomic organization and phylogenetic utility of deer mouse (peromyscus maniculatus) lymphotoxin-alpha and lymphotoxin-beta.deer mice (peromyscus maniculatus) are among the most common mammals in north america and are important reservoirs of several human pathogens, including sin nombre hantavirus (snv). snv can establish a life-long apathogenic infection in deer mice, which can shed virus in excrement for transmission to humans. patients that die from hantavirus cardiopulmonary syndrome (hcps) have been found to express several proinflammatory cytokines, including lymphotoxin (lt), in the lungs. it is thought that t ...200818976466
first genetic detection of tula hantavirus in wild rodents in the netherlands. 200818977537
hantavirus antibodies in rodents and human cases with pulmonary syndrome, rio negro, argentina.in río negro province, argentina, human cases of hantavirus pulmonary syndrome (hps) appeared in the region of subantarctic forests. the andes virus (andv) has been identified in the region both in oligoryzomys longicaudatus rodents and in humans, with the main transmission being from rodents to humans but also showing the possibility of human to human transmission. between 1996 and 2004, in 40 campaigns, 29.960 night-traps for capturing live rodents were set up. blood samples were obtained from ...200818977707
hantavirus cardiopulmonary syndrome: implications for transport management and care.a 14-year-old boy with cardiorespiratory failure was referred for air medical transport. the complexity of care during air medical transport and subsequent diagnosis of hantavirus warranted a post hoc review of the literature to establish optimal transport management criteria.200818992690
natural host relationships and genetic diversity of rodent-associated hantaviruses in southeastern brazil.hantaviruses are rodent-borne rna viruses that have caused hantavirus cardiopulmonary syndrome in several brazilian regions. in the present study, geographical distribution, seroprevalence, natural host range, and phylogenetic relations of rodent-associated hantaviruses collected from seven counties of southeastern brazil were evaluated.200819001829
a hantavirus causing hemorrhagic fever with renal syndrome requires gc1qr/p32 for efficient cell binding and infection.hantaan virus (htnv) is a pathogenic hantavirus that causes hemorrhagic fever with renal syndrome (hfrs). htnv infection is mediated by alpha v beta3 integrin. we used protein blots of vero e6 cell homogenates to demonstrate that radiolabeled htnv virions bind to gc1qr/p32, the acidic 32-kda protein known as the receptor for the globular head domain of complement c1q. rnai-mediated suppression of gc1qr/p32 markedly reduced htnv binding and infection in human lung epithelial a549 cells. conversel ...200818834607
first detection of tula hantaviruses in microtus arvalis voles in hungary.tula hantavirus (tulv) is a member of the genus hantavirus, family bunyaviridae and is mainly carried by the european common vole (microtus arvalis). in order to detect tulv, we tested microtus arvalis (mar) and microtus subterraneus (msu) voles captured in two different locations of the southern transdanubian region of hungary. the viral genome was detectable in 37% of the tested mar voles but, interestingly, was absent in all msu. phylogenetic analysis performed with a partial coding sequence ...200818836679
hantavirus-induced immunity in rodent reservoirs and humans.summary: hantaviruses are predominantly rodent-borne pathogens, although recently novel shrew-associated hantaviruses were found. within natural reservoir hosts, hantairuses do not cause obvious pathogenetic effects; transmission to humans, however, can lead to hemorrhagic fever with renal syndrome or hantavirus cardiopulmonary syndrome, depending on the virus species involved. this review is focussed on the recent knowledge on hantavirus-induced immune responses in rodent reservoirs and humans ...200818837782
[study on the molecular epidemiology of hantavirus carried by rodent hosts in cixi, zhejiang province].to collect more data on the epidemiology of hantavirus in rodents in cixi, zhejiang province.200818843995
[study on the molecular characteristic of natural infection of rodents with hantaviruses in shenzhen city].in order to investigate hantavirus (hv) infection of captured rodents and to understand the genotypes and the molecular characteristic of hantaviruses in shenzhen.200818844081
molecular phylogeny of a newfound hantavirus in the japanese shrew mole (urotrichus talpoides).recent molecular evidence of genetically distinct hantaviruses in shrews, captured in widely separated geographical regions, corroborates decades-old reports of hantavirus antigens in shrew tissues. apart from challenging the conventional view that rodents are the principal reservoir hosts, the recently identified soricid-borne hantaviruses raise the possibility that other soricomorphs, notably talpids, similarly harbor hantaviruses. in analyzing rna extracts from lung tissues of the japanese sh ...200818854415
hantaviruses and tnf-alpha act synergistically to induce erk1/2 inactivation in vero e6 cells.we have previously reported that the apathogenic tula hantavirus induces apoptosis in vero e6 epithelial cells. to assess the molecular mechanisms behind the induced apoptosis we studied the effects of hantavirus infection on cellular signaling pathways which promote cell survival. we previously also observed that the tula virus-induced cell death process is augmented by external tnf-alpha. since tnf-alpha is involved in the pathogenesis of hantavirus-caused hemorrhagic fever with renal syndrome ...200818822184
corticosteroids modulate seoul virus infection, regulatory t-cell responses and matrix metalloprotease 9 expression in male, but not female, norway rats.human hantaviral disease is mediated by excessive proinflammatory and cd8+ t-cell responses, which can be alleviated by administration of corticosteroids. in contrast with humans, male rats that are infected with their species-specific hantavirus, seoul virus (seov), have reduced proinflammatory and elevated regulatory t-cell responses in tissues where virus persists. to determine the effects of glucocorticoids on seov persistence and immune responses during infection, male and female norway rat ...200818931068
surveillance of vector-borne diseases in germany: trends and challenges in the view of disease emergence and climate change.the changing epidemiology of vector-borne diseases represents a growing threat to human health. contemporary surveillance systems have to adapt to these changes. we describe temporal trends and geographic origins of vector-borne diseases in germany with regard to strengths of existing disease surveillance and to areas marked for improvement. we focused on hantavirus infection (endemic in germany), chikungunya fever (recently emerging in europe) and dengue fever (imported from tropical regions), ...200819030882
network "rodent-borne pathogens" in germany: longitudinal studies on the geographical distribution and prevalence of hantavirus infections.hantavirus infections are known in germany since the 1980s. while the overall antibody prevalence against hantaviruses in the general human population was estimated to be about 1-2%, an average of 100-200 clinical cases are recorded annually. in the years 2005 and 2007 in particular, a large increase of the number of human hantavirus infections in germany was observed. the most affected regions were located in the federal states of baden-wuerttemberg, bavaria, north rhine westphalia, and lower s ...200819030894
emergence of hantavirus in south germany: rodents, climate and human infections.human hantavirus (serotype puumala) infections are prevalent throughout europe. the bank vole is the main reservoir of the puumala virus (puuv). between 2001 and 2006, the annual incidences in germany ranged from 0.1 to 0.5 per 100,000 inhabitants. about half of the cases were reported from the state of baden-württemberg (bw) in southwest germany. in 2007, 1,089 puuv infections were reported from bw. this reflects an incidence of 10.1:100,000, which is more than 11 times higher than the mean inc ...200819030895
[hantavirus mucosal vaccine through different mucosal with heat-labile enterotoxin b subunit as adjuvants].to evaluation the effect of different mucosal vaccination pathway on hantavirus with the recombinant e. coli heat-labile enterotoxin b subunit (rltb) as adjuvant.200819031694
[the reactivity of sera from hemorrhagic fever in patients with renal syndromes to the recombination nucleotide proteins from european hantaviruses in hubei province].five yeast-expressed recombination nucleotide proteins of european hantaviruses were prepared as coated antigens to detect hantavirus-specific antibodies in sera from hemorrhagic fever with renal syndromes (hfrs) in hubei province, through elisa assay. the relativity among hantaviruses prevailing in different areas was investigated.200819040041
antiviral effect of human saliva against hantavirus.hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome are zoonotic diseases caused by rodent borne hantaviruses. transmission to humans occurs usually by inhalation of aerozolized virus-contaminated rodent excreta. although human-to-human transmission of andes hantavirus has been observed, the mode of transmission is currently not known. saliva from puumala hantavirus (puuv)-infected patients was shown recently to contain viral rna. to test if human saliva interferes wit ...200819040288
immunological mechanisms mediating hantavirus persistence in rodent reservoirs.hantaviruses, similar to several emerging zoonotic viruses, persistently infect their natural reservoir hosts, without causing overt signs of disease. spillover to incidental human hosts results in morbidity and mortality mediated by excessive proinflammatory and cellular immune responses. the mechanisms mediating the persistence of hantaviruses and the absence of clinical symptoms in rodent reservoirs are only starting to be uncovered. recent studies indicate that during hantavirus infection, p ...200819043585
sin nombre virus infection in deer mice, channel islands, california. 200819046540
storage of cellular 5' mrna caps in p bodies for viral cap-snatching.the minus strand and ambisense segmented rna viruses include multiple important human pathogens and are divided into three families, the orthomyxoviridae, the bunyaviridae, and the arenaviridae. these viruses all initiate viral transcription through the process of "cap-snatching," which involves the acquisition of capped 5' oligonucleotides from cellular mrna. hantaviruses are emerging pathogenic viruses of the bunyaviridae family that replicate in the cytoplasm of infected cells. cellular mrnas ...200819047634
[increased incidence of hantavirus infection in germany and austria]. 200818937155
genetic immunization with hantavirus vaccine combining expression of g2 glycoprotein and fused interleukin-2.in this research, we developed a novel chimeric htnv-il-2-g2 dna vaccine plasmid by genetically linking il-2 gene to the g2 segment dna and tested whether it could be a candidate vaccine. chimeric gene was first expressed in eukaryotic expression system pcdna3.1 (+). the htnv-il-2-g2 expressed a 72 kda fusion protein in cos-7 cells. meanwhile, the fusion protein kept the activity of its parental proteins. furthermore, balb/c mice were vaccinated by the chimeric gene. elisa, cell microculture neu ...200818940009
roles for the recycling endosome, rab8, and rab11 in hantavirus release from epithelial cells.hantavirus structural proteins are believed to localize to intracellular membranes often identified as golgi membranes, in virus-infected cells. after virus budding into the golgi luminal space, virus-containing vesicles are transported to the plasma membrane via trafficking pathways that are not well defined. using the new world hantavirus, andes virus, we have investigated the role of various rab proteins in the release of hantavirus particles from infected cells. rabs 8 and 11 were found to c ...200818951604
the role of heterogeneity in the persistence and prevalence of sin nombre virus in deer mice.many diseases persist at a relatively low prevalence, seemingly close to extinction. for a chronic disease in a homogeneous population, reducing the transmission rate by a fraction proportional to the prevalence would be sufficient to eradicate the disease. this study examines how higher prevalence of the sin nombre virus in male deer mice (peromyscus maniculatus) might contribute to disease persistence. analyzing data from over 2,000 individual mice captured in 19 sites over 4 years, we found p ...200818959490
[the dynamics of t lymphocyte subsets in hemorrhagic fever with renal syndrome].to investigate the t cell subsets changes in hemorrhagic fever with renal syndrome (hfrs) patients.200819080298
genetic characterization and phylogeny of a hantavirus from western mexico.hantaviruses can cause two serious illnesses when transmitted from their rodent reservoirs to humans; hantavirus pulmonary syndrome (hps) in the new world and hemorrhagic fever with renal syndrome in the old world. cases of hps were first recognized in the americas in small, focal outbreaks in rural populations in the southwestern usa in 1993. since that time, outbreaks as well as sporadic cases of hps have been recognized throughout the americas. remarkably, hps cases have not been reported in ...200817963942
a soluble and heat-resistant form of hantavirus nucleocapsid protein for the serodiagnosis of hfrs.hemorrhagic fever with renal syndrome (hfrs) is a rodent-borne viral zoonosis characterized by fever, hemorrhagic manifestations, and renal disorder. the causative agent of hfrs has been identified as a hantavirus. hantavirus nucleocapsid proteins have recently been shown to be immunodominant antigens in hfrs, inducing an early and long-lasting immune response, and their amino termini are sensitive tools for the detection of specific antibodies in hfrs patient sera. previous work has demonstrate ...200817826847
kinship, dispersal and hantavirus transmission in bank and common voles.hantaviruses are among the main emerging infectious agents in europe. their mode of transmission in natura is still not well known. in particular, social features and behaviours could be crucial for understanding the persistence and the spread of hantaviruses in rodent populations. here, we investigated the importance of kinclustering and dispersal in hantavirus transmission by combining a fine-scale spatiotemporal survey (4 km2) and a population genetics approach. two specific host-hantavirus s ...200818071626
differential regulation of pathogens: the role of habitat disturbance in predicting prevalence of sin nombre virus.deer mice (peromyscus maniculatus) are the primary reservoir for sin nombre virus (snv), a north american hantavirus that causes disease with high mortality in humans. recent studies have proposed that habitat disturbance affects prevalence of snv in deer mice; however, the outcomes proposed in these studies are in opposition to each other. our objectives were to test these divergent hypotheses by: (1) measuring snv infection in deer mice within a patchwork of disturbance; and (2) evaluating the ...200818064494
seroepidemiological study on hantavirus infections in india.hantaviruses are etiological agents of hemorrhagic fever with renal syndrome in many parts of asia and europe. there has been no documented case of hantavirus disease from india, although serological evidence exists. we investigated the prevalence of hantavirus in the indian population and tried to identify potential risk groups for hantavirus infections. the presence of hantavirus-specific igg antibodies was prospectively evaluated in 661 subjects belonging to different groups, i.e. patients wi ...200817996913
immune serum produced by dna vaccination protects hamsters against lethal respiratory challenge with andes virus.hantavirus pulmonary syndrome (hps) is a highly pathogenic disease (40% case fatality rate) carried by rodents chronically infected with certain viruses within the genus hantavirus of the family bunyaviridae. the primary mode of transmission to humans is thought to be inhalation of excreta from infected rodents; however, ingestion of contaminated material and rodent bites are also possible modes of transmission. person-to-person transmission of hps caused by one species of hantavirus, andes viru ...200818032485
profiling of external metabolites during production of hantavirus nucleocapsid protein with recombinant saccharomyces cerevisiae.recombinant strains of saccharomyces cerevisiae, producing hantavirus puumala nucleocapsid protein for diagnostics and as a candidate vaccine were analyzed for uptake and excretion of intermediary metabolites during process optimization studies of fed-batch bioreactor cultures. concentrations of glucose, maltose, galactose, pyruvate, acetaldehyde, ethanol, acetate, succinate and formaldehyde (used as a selection agent) were measured in the culture medium in order to find a metabolite pattern, in ...200817985082
how host population dynamics translate into time-lagged prevalence: an investigation of sin nombre virus in deer mice.human cases of hantavirus pulmonary syndrome caused by sin nombre virus are the endpoint of complex ecological cascade from weather conditions, population dynamics of deer mice, to prevalence of snv in deer mice. using population trajectories from the literature and mathematical modeling, we analyze the time lag between deer mouse population peaks and peaks in snv antibody prevalence in deer mice. because the virus is not transmitted vertically, rapid population growth can lead initially to redu ...200817701378
association between the dqa mhc class ii gene and puumala virus infection in myodes glareolus, the bank vole.puumala virus (puuv) is a hantavirus specifically harboured by the bank vole, myodes (earlier clethrionomys) glareolus. it causes a mild form of hemorrhagic fever with renal syndrome (hfrs) in humans, called nephropathia epidemica (ne). the clinical severity of ne is variable among patients and depends on their major histocompatibility complex (mhc) genetic background. in this study we investigated the potential role of class ii mhc gene polymorphism in the susceptibility/resistance to puuv in t ...200817693139
effect of predators of juvenile rodents on the spread of the hantavirus epidemic.effects of predators of juvenile mice on the spread of the hantavirus are analyzed in the context of a recently proposed model. two critical values of the predation probability are identified. when the smaller of them is exceeded, the hantavirus infection vanishes without extinguishing the mice population. when the larger is exceeded, the entire mice population vanishes. these results suggest the possibility of control of the spread of the epidemic by introducing predators in areas of mice colon ...200817694347
[detection of anti-hantavirus antibodies in forest workers in the north-east of poland].to estimate exposition to hantaviruses in a high-risk population (forest workers) in the north-east of poland.200819108515
[molecular epidemiological study on the host and role of the hantavirus and orientia tsutsugamushi in the same epidemic area].to investigate whether hantavirus (hv) and orientia tsutsugamushi ( ot) can naturally infect and coexist in their host and role.200819115620
the effect of habitat fragmentation and species diversity loss on hantavirus prevalence in panama.habitat fragmentation and diversity loss due to increased conversion of natural habitats to agricultural uses influence the distribution and abundance of wildlife species and thus may change the ecology of pathogen transmission. we used hantaviruses in panama as a research model to determine whether anthropogenic environmental change is associated with changes in the dynamics of viral transmission. specifically, we wanted to determine whether hantavirus infection was correlated with spatial attr ...200819120179
recent discoveries of new hantaviruses widen their range and question their origins.hantaviruses belong to the bunyaviridae family. while usually hosted by wild mammals, they are potentially pathogenic for humans, and several serologically distinct groups associated with different syndromes have been identified. yet, investigations have mostly been conducted where human infections by hantaviruses constitute a real and well-identified public health problem, i.e., the holarctic and neotropical areas. some hantaviruses have also been described from a suncus murinus in india and a ...200819120180
expression of l protein of hantaan virus 84fli strain and its application for recovery of minigenomes.hantaan virus (htnv) is an old world hantavirus associated with hemorrhagic fever with renal syndrome (hfrs). to visualize the localization of the l protein of htnv strain 84fli within cells, a fusion protein composed of enhanced green fluorescent protein and l protein, egfp-l, was expressed in vero cells. the 273 kda expressed fusion protein of egfp-l localized in the perinuclear region. we also described the development of a reverse genetics system for htnv strain 84fli. the rna polymerase i ( ...200819133012
[investigation of modes of hantavirus infection transmission from rodents to humans].hemorrhagic fever with renal syndrome (hfrs) is a focal zoonosis of viral etiology that appears in many countries of the world. investigation demonstrated that for epidemic of hfrs is necessary to have intensity of epizootic process. the increased number of mice-like rodents in enzootic foci of hfrs is the most important factor. this investigation pointed out to high risk of direct contact with rodents secrets, from visible urine and feces traces of rodents that are present in large number of pa ...200819145808
[the molecular epidemiologic investigation of hantavirus carried by rodent hosts in wenzhou, zhejiang province].to study the epidemiological features of hantavirus in rodents in wenzhou, zhejiang province.200819173853
ecological surveillance of small mammals at firing points 10 and 60, gyeonggi province, republic of korea, 2001-2005.throughout korea, small mammals are hosts to a number of disease-causing agents that pose a health threat to u.s. and korean military forces while they conduct field-training exercises. a seasonal rodent-borne disease surveillance program was established at two firing points (fp), fp-10, and fp-60, and conducted over five years from 2001 through 2005 in response to hantavirus cases among u.s. soldiers. the ecology of these sites consisted primarily of tall grasses associated with semi-permanent ...200819263858
new genetic lineage of tula hantavirus in microtus arvalis obscurus in eastern kazakhstan.genomic sequences of tula (tulv) hantavirus were recovered from tissue samples of european common voles microtus arvalis (subspecies obscurus) captured in kazakhstan, central asia. phylogenetic analysis of the s genomic segment of kazakh tulv strains showed that they form distinct, well supported genetic lineage and share a more ancient common ancestor with two russian lineages of tulv. the deduced sequence of the nucleocapsid (n) protein of kazakh tulv strains carried specific amino acid signat ...200819440462
hantaviruses: an emerging public health threat in india? a review.the emerging viral diseases haemorrhagic fever with renal syndrome (hfrs) and hantavirus cardiopulmonary syndrome (hcps) are a cause of global concern as they are increasingly reported from newer regions of the world. the hantavirus species causing hfrs include hantaan virus,seoul virus, puumala virus, and dobrava-belgrade virus while sin nombre virus was responsible for the 1993 outbreak of hcps in the four corners region of the us. humans are accidental hosts and get infected by aerosols gener ...200819208975
[molecular characterization and sequence comparison of the m and s segments of hantavirus zj5 strain].in order to understand the molecular characters of hantavirus zj5 strain, its complete m and s genome were sequenced and compared with that of other hantavirus strains.200819544637
[identification and analysis of an seo strain of hemorrhagic fever with renal syndrome hantavirus from guangxi].to identify hantavirus from lung specimens of rats captured in guangxi.200819544640
hantavirus infection: a review and global update.hantaviruses have the potential to cause two different types of diseases in human: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). hfrs, initially described clinically at the turn of the 20th century, occurs endemically in the asian and european continents, while hps, recognized as a clinical entity since 1993, represents the prototype of emerging diseases occurring in the western hemisphere. approximately 150,000 to 200,000 cases of hfrs are hospitalized ea ...200819736383
barn owl (tyto alba) predation on small mammals and its role in the control of hantavirus natural reservoirs in a periurban area in southeastern brazil.the aim of this study was to inventory the species of small mammals in uberlândia, minas gerais, brazil, based on regurgitated pellets of the barn owl and to compare the frequency of rodent species in the diet and in the environment. since in the region there is a high incidence of hantavirus infection, we also evaluate the importance of the barn owl in the control of rodents that transmit the hantavirus. data on richness and relative abundance of rodents in the municipality were provided by the ...200819197490
infections in the natural environment of british columbia, canada.the canadian province of british columbia has a luxurious environment, complete with the multitude of wildlife and insects, and would at first glance appear to be suitable for the transmission of diseases in nature communicable to humans. despite this potential, such diseases are relatively uncommon, although several have the potential for serious consequences. attention has been recently focused on hantavirus infection, water-borne toxoplasmosis and parasitic diarrheal diseases, cryptococcosis ...200820701841
isolation and characterization of microsatellite markers in oligoryzomys longicaudatus (muridae, sigmodontinae, oryzomini), the natural reservoir of genotype andes hantavirus.the rodent oligoryzomys longicaudatus or long-tailed pygmy rice rat is the reservoir of the aetiological agent of the hantavirus pulmonary syndrome in southern argentina and chile. we characterize 11 polymorphic microsatellite loci which would be useful for studies on microgeographical population structure in the species. amplification of these loci in 42 individuals from four natural populations revealed four to 21 alleles per locus, and values of observed heterozygosities ranging from 0.371 to ...200821586078
[expression of partial s gene of hantavirus z10 in pichia pastoris].the purpose of this study is to express partial s gene of hantavirus z10.200920718348
an experimental test of factors attracting deer mice into buildings.deer mice (peromyscus maniculatus) are the principal reservoir host of sin nombre virus (snv). deer mice use a wide variety of habitats including peridomestic settings in and around human dwellings, their presence in and around homes has been implicated as a risk factor for acquiring hantavirus pulmonary syndrome. deer mice are believed to enter buildings in order to gain access to a variety of resources including food, bedding material, and better thermal microclimates. however, no one has expe ...200920628475
bayou virus detected in non-oryzomyine rodent hosts: an assessment of habitat composition, reservoir community structure, and marsh rice rat social dynamics.in the united states, bayou virus (bayv) ranks second only to sin nombre virus (snv) in terms of hantavirus pulmonary syndrome (hps) incidents, having been confirmed in cases from texas and louisiana since its discovery in 1994. this study on bayv infection among sympatric, non-oryzomyine rodents ("spillover") in freeport, tx, is the first to link patterns of hantavirus interspecific spillover with the spatiotemporal ecology of the primary host (marsh rice rat, oryzomys palustris). mark-recaptur ...200920836801
microhabitat characteristics of akodon montensis, a reservoir for hantavirus, and hantaviral seroprevalence in an atlantic forest site in eastern paraguay.hantaviruses may cause serious disease when transmitted to humans by their rodent hosts. since their emergence in the americas in 1993, there have been extensive efforts to understand the role of environmental factors on the presence of these viruses in their host rodent populations. hps outbreaks have been linked to precipitation, but climatic factors alone have not been sufficient to predict the spatial-temporal dynamics of the environment-reservoir-virus system. using a series of mark-recaptu ...200920836810
simultaneous detection of cdc category "a" dna and rna bioterrorism agents by use of multiplex pcr & rt-pcr enzyme hybridization assays.assays to simultaneously detect multiple potential agents of bioterrorism are limited. two multiplex pcr and rt-pcr enzyme hybridization assays (mpcr-eha, mrt-pcr-eha) were developed to simultaneously detect many of the cdc category "a" bioterrorism agents. the "bio t" dna assay was developed to detect: variola major (vm), bacillus anthracis (ba), yersinia pestis (yp), francisella tularensis (ft) and varicella zoster virus (vzv). the "bio t" rna assay (mrt-pcr-eha) was developed to detect: ebola ...200920224751
healthcare-associated atypical pneumonia.atypical pneumonia was first described in 1938, and over time, mycoplasma, legionella, and chlamydophila were the agents commonly linked with community-associated atypical pneumonia. however, as technology has improved, so has our understanding of this clinical entity. it is now known that there are many agents linked with atypical pneumonia in the community, and many of these agents are also major causes of healthcare-associated pneumonia. this article discusses the history, epidemiology, and p ...200919199189
survey for antibody to hantaviruses in tamaulipas, mexico.wild rodents (n=248) were trapped in two ecologically distinct sites at el cielo biosphere reserve in the state of tamaulipas, mexico, during the summer of 2003. samples from 199 individuals were tested for hantavirus antibodies by an indirect enzyme-linked immunosorbent assay (elisa). hantavirus antibodies to recombinant sin nombre virus nucleocapsid protein were found in seven rodents (3.5%) of a single species, peromyscus levipes. antibody-positive rodents were found only in the cloud forest ...200919204351
molecular diagnostics of hemorrhagic fever with renal syndrome during a dobrava virus infection outbreak in the european part of russia.a large outbreak of hemorrhagic fever with renal syndrome (hfrs) occurred in the winter of 2006-2007 in a region southeast of moscow in central european russia. of the 422 patients with hfrs investigated in this study, 58 patients were found to be infected by puumala virus, whereas as many as 364 were infected by dobrava-belgrade virus (dobv). early serum samples from 10 dobv-infected patients were used for nucleic acid amplification, which was successful for 5 patients. molecular analyses demon ...200919828747
using satellite images of environmental changes to predict infectious disease outbreaks.recent events clearly illustrate a continued vulnerability of large populations to infectious diseases, which is related to our changing human-constructed and natural environments. a single person with multidrug-resistant tuberculosis in 2007 provided a wake-up call to the united states and global public health infrastructure, as the health professionals and the public realized that today's ease of airline travel can potentially expose hundreds of persons to an untreatable disease associated wit ...200919788799
hantavirus infection in the republic of georgia.we describe a laboratory-confirmed case of hantavirus infection in the republic of georgia. limited information is available about hantavirus infections in the caucasus, although the infection has been reported throughout europe and russia. increasing awareness and active disease surveillance contribute to our improved understanding of the geographic range of this pathogen.200919788822
hantavirus pulmonary syndrome in brasilia periphery: a diagnostic challenge.hantavirus pulmonary syndrome (hps) is an emerging rodent-borne disease in the american continent, characterized by acute respiratory distress and a high case-fatality ratio. the present work describes a case of hps, with favorable outcome, whose initial features were mistaken for leptospirosis or other less severe acute infections.200919801809
hantavirus species in india: a retrospective study.hantaviruses cause hemorrhagic fever with renal syndrome in europe and asia. there are about 20 documented hantavirus species and newer species are being described worldwide, especially in non-rodent reservoirs, i.e shrews. focus reduction neutralization test is the classical serotyping technique for hantavirus. however, this study employs a previously established serotyping elisa, to retrospectively analyze known hantavirus igg reactive samples for infecting serotypes. the result suggests prese ...200919736405
[serological characteristics of hantaviruses from clinical specimens analyzed in 1998-2008 in the department of virology, public health institute, ostrava].in 1998 - 2008, serum samples from 464 patients, predominantly residents of the moravia-silesian region, were examined in the department of virology, public health institute in ostrava, using enzyme-linked immunosorbent assay (elisa) for the detection of specific anti-hantavirus antibodies. seropositivity was ascertained in 41 persons (8.8%). more patients tested seropositive against hantaan virus (24, i.e. 58.5%) than against puumala virus (13, i.e. 31.7%). four persons were reactive against bo ...200919750821
inhibition of tnf-alpha-induced activation of nf-kappab by hantavirus nucleocapsid proteins.hantaviruses cause two human diseases thought to be immune-mediated: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). we recently reported that the nucleocapsid (n) protein of hfrs-causing hantaan virus (htnv) inhibits tumor necrosis factor-alpha (tnf-alpha) activation of nuclear factor-kappab (nf-kappab). here we measured the ability of other hantaviral n proteins to similarly interfere with the inflammatory process of tnf-alpha. we found that like htnv n, t ...200919751407
identification of oligopeptides mimicking the receptor-binding domain of hantaan virus envelope glycoprotein from a phage-displayed peptide library.attachment of enveloped viruses to cells is triggered by the receptor-binding domain (rbd) on envelope glycoproteins (gp) binding to receptors located on the cell surface. to date, recognized receptors and rbd of hantaan virus (htnv) have not been exactly defined. in this study, one monoclonal antibody (mab) 3g1 possessing high neutralizing activity, which is directed against htnv envelope glycoprotein g2, was used to determine the crucial motif of rbd. peptide ligands binding to mab 3g1 were se ...200919767836
complete nucleotide sequence of four rna segments of fig mosaic virus.the complete sequence of four viral rna segments of fig mosaic virus (fmv) was determined. each of the four rnas comprises a single open reading frame (orf) 7,093, 2,252, 1,490 and 1,472 nucleotides in size, respectively. these orfs encode the following proteins in the order: rna-dependent rna polymerase (p1 264 kda), a putative glycoprotein (p2 73 kda), a putative nucleocapsid protein (p3 35 kda) and a protein with unknown function (p4 40.5 kda). all rna segments possess untranslated regions co ...200919777155
evaluation of an enzyme-linked immunosorbent assay based on araraquara virus recombinant nucleocapsid protein.laboratory diagnosis of hantavirus cardiopulmonary syndrome (hcps) in brazil has been performed mostly by detection of igm antibodies to recombinant antigen purified from sin nombre virus and andes virus (andv). recently, a recombinant nucleocapsid (rn) protein of araraquara virus (arav), a brazilian hantavirus, was obtained in escherichia coli. to evaluate arav rn as antigen for antibody detection, serum samples from 30 patients from argentina seropositive for hantavirus were tested. all sample ...200919635882
[survey on host animal and molecular epidemiology of hantavirus in chuxiong prefecture, yunnan province].to determine the hosts of hantavirus (hv) and its molecular epidemiological characteristics, to provide evidence for prevention and control on hemorrhagic fever with renal syndrome (hfrs).200919642377
production and characterization of monoclonal antibodies against the recombinant nucleoprotein of araucaria hantavirus.hantaviruses are rodent-borne rna viruses that cause hemorrhagic fever with renal syndrome (hfrs) or hantavirus pulmonary syndrome (hps). from the first detection of infection in brazil in 1993 until 2009, 1161 cases of hps have been reported, with mortality rates of around approximately 40%. currently, due to the absence of a vaccine or specific antiviral therapy, the only way to reduce mortality by hantavirus infection is a fast and precise diagnosis that allows for supportive clinical health ...200919654026
genetic characterization of a juquitiba-like viral lineage in oligoryzomys nigripes in rio de janeiro, brazil.hantaviruses, family bunyaviridae, are rodent-borne rna viruses that have caused cases of hantavirus cardiopulmonary syndrome (hcps) in various regions of the americas. there are five hantaviral lineages associated with hcps in brazil: juquitiba virus (juqv), araraquara virus (arav), laguna negra-like virus (lnv), castelo dos sonhos virus (casv), and anajatuba virus (anajv). three additional hantaviruses have been described in rodents alone: rio mearim virus, jaborá virus, and a hantavirus linea ...200919660427
degradation and aggresome formation of the gn tail of the apathogenic tula hantavirus.the cytoplasmic tails of envelope glycoprotein gn of pathogenic hantaviruses but not of the apathogenic prospect hill virus (phv) were recently reported to be proteasomally degraded in simian cos7 cells. here, we show that the cytoplasmic tails of the glycoproteins of the apathogenic hantaviruses tula virus (tulv) and phv are also degraded through the ubiquitin-proteasome pathway, both in human hek-293 and in simian vero e6 cells. tulv gn tails formed aggresomes in cells with proteasomal inhibit ...200919675185
[pulmonary and cardiovascular syndrome due to hantavirus: clinical aspects of an emerging disease in southeastern brazil].pulmonary and cardiovascular syndrome due to hantavirus is a disease caused by inhalation of aerosols from the excreta of wild rodents contaminated by viruses of the bunyaviridae family. we studied the clinical and laboratory manifestations of 70 cases that occurred in the region of ribeirão preto, sp, brazil, between 1998 and 2007. the frequency of symptoms was as follows: dyspnea (87%), fever (81%), coughing (44%), headache (34%), tachycardia (81%), low arterial blood pressure (56%), metabolic ...200919684976
domestically acquired seoul virus causing hemorrhagic fever with renal syndrome-maryland, 2008.hantaviruses are rodent-borne viruses capable of causing human disease. the seoul virus is a hantavirus that causes hemorrhagic fever with renal syndrome in east asia. to our knowledge, we report the first domestically acquired case of hemorrhagic fever with renal syndrome caused by the seoul virus, confirmed by serology testing, reverse-transcriptase polymerase chain reaction, and nucleotide sequence analysis. the patient presented with myalgias and fever, and developed acute renal failure.200919848600
co-circulation of three pathogenic hantaviruses: puumala, dobrava, and saaremaa in hungary.hantaviruses (bunyaviridae) cause hemorrhagic fever with renal syndrome (hfrs) in eurasia and hantavirus (cardio)pulmonary syndrome (hcps) in the americas. hfrs is caused by hantaan virus (htnv), seoul virus (seov), dobrava virus (dobv), saaremaa virus (saav), and puumala virus (puuv). of those, only htnv is not present in europe. in recent years, hantaviruses, described in other parts of europe, were also detected at various locations in hungary. to study the genetic properties of hungarian han ...200919856478
[infectious diseases and climate change].climate changes will likely have an impact on the spectrum of infectious diseases in europe. we may see an increase in vector-borne diseases, diseases spread by rodents such as hantavirus, and food- and water-borne diseases. as the effects of climate changes are likely to occur gradually, a modern industrialised country such as denmark will have the opportunity to adapt to the expected changes.200919857396
vaccines for hantaviruses.hantaviruses are assigned to categories a and c on the national institute of allergy and infectious diseases (niaid) priority pathogens list and to category c on the centers for disease control and prevention (cdc) emergency preparedness and response list of boterrorism agents/diseases. these rodent-borne viruses are members of the family bunyaviridae and are transmitted to humans in aerosols of rodent excreta. there are more than 20 recognized hantaviruses, some of which are associated with one ...200919837289
molecular epidemiological and serological studies of hantavirus infection in northern vietnam.the distribution of anti-hantavirus antibodies in humans and rodents in northern vietnam was examined. in total, 837 serum samples from healthy humans (617) and patients with fever (220), living in six different areas were screened for igg antibodies against hantaan or seoul virus (seov) by elisa, ifa, and western blot analysis. antibody-positive sera were identified in 7/617 (1.1%) healthy donors, 5/150 port workers in the port of hai phong, and 2/185 residents of ha nam province. in comparison ...200919887743
[research on hantanvirus infection of rodents in junan county, shandong province]. 200919957614
sympatry of 2 hantavirus strains, paraguay, 2003-2007.to explore geographic and host-taxonomic patterns of hantaviruses in paraguay, we established sampling sites in the mbaracayu biosphere reserve. we detected jabora virus and itapua37/juquitiba-related virus in locations approximately 20 m apart in different years, which suggested sympatry of 2 distinct hantaviruses.200919961679
cross-sectional survey of hantavirus infection, brazil.a cross-sectional serosurvey was conducted to assess the proportion of persons exposed to hantaviruses in a virus-endemic area of the state of minas gerais, brazil. findings of this study suggested the presence of > or =1 hantaviruses circulating in this region causing hantavirus pulmonary syndrome, mild disease, or asymptomatic infection.200919961680
dobrava-belgrade virus spillover infections, germany.we present the molecular identification of apodemus agrarius (striped field mouse) as reservoir host of the dobrava-belgrade virus (dobv) lineage dobv-aa in 3 federal states of germany. phylogenetic analyses provided evidence for multiple spillover of dobv-aa to a. flavicollis, a crucial prerequisite for host switch and genetic reassortment.200919961690
hantavirus regulation of endothelial cell functions.hantaviruses cause two vascular permeability-based diseases and primarily infect endothelial cells which form the primary fluid barrier of the vasculature. since hantavirus infections are not lytic, the mechanisms by which hantaviruses cause haemorrhagic fever with renal syndrome (hfrs) or hantavirus pulmonary syndrome (hps) are indeterminate. hps is associated with acute pulmonary oedema and hfrs with moderate haemorrhage and renal sequelae, perhaps reflecting the location of vast microvascular ...200919967132
[thrombocytopenia and acute kidney injury].a 31-year old man is addressed to the emergency department for fever, abdominal pain and vomiting. laboratory tests reveal an inflammatory syndrome, thrombocytopenia, acute kidney injury associated with major proteinuria (more than 3 g/24 h). evaluation will conclude to an acute interstitial nephritis and the serology is positive for hantavirus. this clinical case illustrates differential diagnosis of acute renal failure and reminds the theory of hemorrhagic fever with renal syndrome : usual cli ...200919998799
[from mice to men: the threat of hantavirus infections].hantaviruses are naturally hosted by small mammals. virus transmission to humans can result in the development of hemorrhagic fever with renal syndrome (asia, europe) or hantavirus cardiopulmonary syndrome (the americas). certain hantaviruses can cause life-treatening infections with case fatality indices up to about 50%. different virus types have evolved which use different species of small mammals (mainly but not exclusively rodent species) as natural reservoirs. it is still unknown why these ...200919999378
seasonal dispersal patterns of sylvan deer mice (peromyscus maniculatus) within montana rangelands.we examined seasonal dispersal patterns and timing of new infections of sin nombre virus (snv), as determined by recent acquisition of antibodies (seroconversion), in deer mice (peromyscus maniculatus) at two montana rangeland study sites over three years, 2004-2007. one study site was located in grassland habitat, and the other was located in shrub-steppe. in montana, both of these habitats are commonly associated with peridomestic environments (in and around buildings). peridomestic environmen ...200919901376
genetic diversity and phylogeography of seewis virus in the eurasian common shrew in finland and hungary.recent identification of a newfound hantavirus, designated seewis virus (swsv), in the eurasian common shrew (sorex araneus), captured in switzerland, corroborates decades-old reports of hantaviral antigens in this shrew species from russia. to ascertain the spatial or geographic variation of swsv, archival liver tissues from 88 eurasian common shrews, trapped in finland in 1982 and in hungary during 1997, 1999 and 2000, were analyzed for hantavirus rnas by reverse transcription-polymerase chain ...200919930716
hantavirus pulmonary syndrome in five pediatric patients - four states, 2009.hantavirus pulmonary syndrome (hps) is a reportable infectious disease with a high case-fatality rate, transmitted to humans by exposure to rodents. each year, 20--40 cases of hps occur in the united states; cases in persons aged <17 years make up fewer than 7% of those cases, and cases in children aged <10 years are exceptionally rare. cdc received reports of five pediatric cases of hps occurring during may 16--november 25, 2009, among children aged 6--14 years from arizona, california, colorad ...200920032925
epidemiological study of hantavirus infection in the samara region of european russia.european russia is a highly endemic area of hemorrhagic fever with renal syndrome (hfrs), a rodent-borne zoonotic disease, caused by hantaviruses. in total, 145 small mammals of four species (myodes glareolus, apodemus flavicollis, a. agrarius, and a. uralensis) were trapped in the samara region of european russia in august 2005 and examined for the presence of hantavirus (hv). anti-hv antibodies were found in six of 68 (8.8%) m. glareolus and in one of 19 (5.3%) a. flavicollis by indirect immun ...200920046023
milder winters in northern scandinavia may contribute to larger outbreaks of haemorrhagic fever virus.the spread of zoonotic infectious diseases may increase due to climate factors such as temperature, humidity and precipitation. this is also true for hantaviruses, which are globally spread haemorrhagic fever viruses carried by rodents. hantaviruses are frequently transmitted to humans all over the world and regarded as emerging viral diseases. climate variations affect the rodent reservoir populations and rodent population peaks coincide with increased number of human cases of hantavirus infect ...200920052429
[geographic expansion of hantavirus pulmonary syndrome in argentina. the southernest case report].since 1995 more than 1000 cases of hantavirus pulmonary syndrome (hps) were reported in argentina, a severe disease and often fatal to humans. most cases were associated with andes virus (and) that caused few events of person-to-person transmission. several lineages of pathogenic and viruses have been described, including and south, hosted by the rodent oligoryzomys longicaudatus which affects the patagonian region of argentina and chile. we studied the clinical and epidemiological characteristi ...200920053606
hsp70 induced by hantavirus infection interacts with viral nucleocapsid protein and its overexpression suppresses virus infection in vero e6 cells.hantavirus (htv) infection is known to induce innate cellular response, a more specified cellular response in the host cells. however, whether it stimulates synthesis of stress proteins, particularly associations of viral proteins, is entirely unknown. the primary focus of this research is using vero e6 cells infected with hantaan 76-118 (htnv) as an in vitro infection model to examine the individual contribution of htv infection to heat shock response. this study shows that htnv infection rapid ...200919956449
perspectives on scrub typhus, tick-borne pathogens, and hantavirus in the republic of korea. 200920088033
[genotyping and sequence analysis on g2 segment of hantavirus carried by rat in hebei province].to know the genotype and subtype of hantavirus (hv) carried by host animals in hfrs natural epidemic area of hebei province.200920104766
the occurrence of hemorrhagic fever with renal syndrome in southern parts of bosnia and herzegovina.bosnia and herzegovina (b&h) has been known as an endemic region for hemorrhagic fever with renal syndrome (hfrs) for over 50 years. multiple epidemics of this disease have been registered so far, especially in endemic parts of central and northeastern bosnia, as well as the sarajevo region. seroepidemiological investigations demonstrate naturalization of hantaviruses and their wide spread in b&h. however, there are no studies from the southern areas of b&h, and endemic foci of this disease are ...200920120399
[construction of a recombinant adenovirus carrying the hantaan virus glycoprotein g2 gene and its expression in hela cells].we constructed the recombinant adenovirus expressing the glycoprotein g2 of hantaan virus. firstly we obtained coding gene fragment of g2 by pcr, and subsequently inserted the gene of interest into the adenoviral pshuttle vector pad5-cmv. then we co-transfected the recombinant pshuttle vector and adenovirus skeleton plasmid into hek293 cells by calcium phosphate precipitation method. after the recombinant adenovirus were packaged and amplified in hek293 cells, we observed the expression of repor ...200920112705
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