Publications

new method for large-scale growth; and concentration of the epstein-barr viruses.	efficacious systems are described for the large-scale growth in tissue culture and concentration of infectious (p3hr-1) and transforming (b95-8) epstein-barr virus. also recorded here are our updated procedures for growing stock cultures and protocols to harvest fluids containing biologically active virus which is infectious or transforming. various methods of concentrating biologically active epstein-barr virus have been evaluated. cellular debris can be removed efficiently and rapidly from cul ...	1980	6257160
effect of short-chain fatty acids on epstein-barr virus early and viral capsid antigen induction in p3hr-1 cells.	the effects of short-chain fatty acids were assayed for their capacity to induce epstein-barr virus (ebv) from the ebv genome-carrying human lymphoblastoid p3hr-1 cells. not only the n-butyric acid, the activity of which is now well established, but also the n-valeric acid was found to induce ebv-associated early antigen (ea) and viral capsid antigen (vca) at an appreciable level. similarly, i-valeric acid showed a considerably lower, but significant, level of this activity, while the i-butyric ...	1980	6257378
[changes in antibody titre after rubella vaccination in primarily rubella-sera positive parturient women. demonstration of antigen-specific (rubella) and non-specific stimulated antibody titres (author's transl)].		1980	6257468
cellular mechanisms of restricted immunoglobulin formation in the human neonate.	the functional capacity of human neonatal b lymphocytes has been investigated by in vitro methods using t lymphocyte-dependent (pokeweek mitogen, pwm) and -independent (epstein-barr virus, ebv) polyclonal b cell activators. b cell activation of single cells was detected by class-specific immunoglobulin (ig) secretion using a reversed hemolytic plaque assay. it was found that neonatal b cells were triggered to secretion of igm by ebv, with a magnitude comparable to adult levels, but that, in cont ...	1980	6257525
[burkitt's tumor].		1980	6257597
[epstein-barr virus infections in pregnancy. the serological profile of active infections in pregnant women. their possible relationship with perinatal mortality (author's transl)].	a significant number of cases of deaths and of perinatal misfortunes are still of unknown origin. the role of pathological infection is probably important in these cases and particularly those in the torch group of viruses, and among these, as has been shown by epidemiological and virological tests, the epstein-barr virus (e.b.v.). the authors have undertaken a prospective study based on serology, looking for e.b.v. antibodies in pregnancy. 1,164 women were studied and 74 of these had perinatal ...	1980	6257774
[cerebellar encephalitis associated with infectious mononucleosis].		1980	6258125
the frequency of epstein-barr virus infection and burkitt's lymphoma at high and low altitudes in east africa.	burkitt's lymphomas (bl) is frequent in the tropical lowland of africa but rare--even in the tropics--at altitudes higher than 5,000 feet above sea level. serological surveys were carried out in uganda and tanzania to see whether the variation in bl incidence from high to low areas is paralleled by a variation in the extent of infection with the putative epstein-barr virus. sera were collected from samples of the general child population living at high and low altitudes in the west nile district ...	1980	6258200
production of human igm anti-d in tissue culture by eb-virus-transformed lymphocytes.	lymphocytes obtained from two donors who were producing anti-d were transformed with epstein-barr virus and grown in tissue culture. the lymphoblasts from both donors produced anti-d antibody, which agglutinated rh-positive cells in saline. both antibodies belonged to the igm class; the concentration of anti-d in the culture fluid from one of the donors was approximately 250--500 ng/ml and bound firmly to rh-positive cells with a functional affinity constant of approximately 1 x 10(9) m-1.	1980	6258221
[non-a, non-b post-transfusion hepatitis. serological survey of 85 patients with acute hepatitis following blood transfusion].	in a serological survey of 85 adult patients hospitalized for an episode of transfusion-associated hepatitis, 45 were reactive for hepatitis b surface antigen and therefore diagnosed as having hepatitis b. forty hbsag non reactive patients were examined for development of antibody to hepatitis a and b antigens, cytomegalovirus and epstein-barr virus. three patients showed serologic evidence of hepatitis b and one for hepatitis a. none of the remaining 36 subjects developed serologic evidence of ...	1980	6258610
induction of epstein-barr virus antigens in human lymphoblastoid p3hr-1 cells with culture fluid of fusobacterium nucleatum.	epstein-barr virus-associated early antigen and viral capsid antigen were efficiently induced in human lymphoblastoid p3hr-1 cells with culture fluid of fusobacterium nucleatum, a member of the indigenous microbial flora of the human host. this finding may suggest a new approach to assess the possible role of the "cofactor(s)" in the etiology of epstein-barr virus-related diseases.	1980	6258779
anti-phosphorylcholine antibody-producing cells in human lymphoblastoid cell lines established by transformation with epstein-barr virus.		1980	6258807
mechanism of epstein-barr virus-induced human b-lymphocyte activation.		1980	6258810
[human fetal-type thymidine kinase isozyme f: purification and biochemical characterization (author's transl)].		1980	6259039
establishment of a cell line (npc/hk1) from a differentiated squamous carcinoma of the nasopharynx.	a long-term cell culture epithelioid cell line was established from a recurrent squamous carcinoma of the nasopharynx of a chinese male 17 1/2 years after radiation therapy. the cell line, designated npc/hk1, has been passed 72 times over a period 1 year. the cells have been shown by light and electron microscopies to be of the squamous epithelial type. when they were transplanted subcutaneously into the back of athymic nude balb/c (nu/nu) mice, tumors developed at the sites of inoculation, whic ...	1980	6259064
epstein-barr virus-specific antibody titers in seven alaskan natives before and after diagnosis of nasopharyngeal carcinoma.	results are presented of epstein-barr virus-specific serologic tests for seven alaskan native patients with nasopharyngeal carcinoma (npc) from whom serum had been collected 2-10 years before diagnosis and up to 6 years after diagnosis. the pre-illness antibody spectra and titers of the npc patients did not differ from those of controls. however, broadened antibody spectra and elevated titers were associated with the emergence of npc disease, which in one case was present as early as 22 months b ...	1980	6259065
epstein-barr virus-related serology in marrow transplant recipients.	serial sera from 50 marrow transplant recipients were examined for their spectra and titers of antibodies to ebv-specific antigens. immediately before or after transplant, blood products passively transferred antibodies to eb viral capsid antigen (vca) and ebv nuclear antigen (ebna). in most recipients, passively-transferred antibodies were replaced by endogenous antibodies regardless of whether donor or recipient had ebv antibodies before transplantation. commencement or resumption of endogenou ...	1980	6259066
biochemical comparison of hla-dr molecules derived from autologous human t and b lymphoblasts.	earlier findings that human activated t blasts display hla-dr determinants have been confirmed. after phytohemagglutinin activation, percoll gradient-purified blast cells were cultured for two weeks in mitogen-derived supernatants from human lymphocyte cultures. using purified t blasts and internal labeling procedures, it was established that t blasts actively produced hla-dr-like chains, as assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. further, hla-dr chains obtained fr ...	1980	6258924
cytomegalovirus-specific immune response and the cellular immune status of malignant lymphoma.	cytomegalovirus (cmv)-specific immune responses and the general cellular immune status of 24 untreated patients with hodgkin's disease (hd) and 22 with non-hodgkin lymphoma (nhl) were evaluated in comparison with matched controls. the incidence of antibodies against cmv-induced early (ea) or late (la) antigens was not different between patients and controls. la antibody titres were markedly lower in the lymphoma patients (p = 0.01), especially in the nhl subgroup (p = 0.008), but not in hd patie ...	1980	6259357
histological and immunopathological studies in a case of chronic epstein-barr virus infection terminating in a burkitt-like lymphoma.	a 6-year-old boy suffered from a severe lymphadenopathy, characterized histologically by a fulminant polyclonal immunoblast proliferation simulating malignant lymphoma with many immunoblasts resembling reed-sternberg cells. he had no history of infectious mononucleosis but epstein-barr virus (ebv) infection was evident from serological findings, and ebv-associated nuclear antigen (ebna) was demonstrated in a high percentage of lymphocytes of blood and lymph nodes. an adequate humoral response to ...	1980	6259716
aspects of specific immunity.		1980	6259719
the activities of thymidine metabolising enzymes during the cell cycle of a human lymphocyte cell line laz-007 synchronised by centrifugal elutriation.	the activities throughout the cell cycle of thymidine kinase (ec 2.7.1.21), dihydrothymine dehydrogenase (ec 1.3.1.2), thymidine phosphorylase (ec 2.4.2.4) and dtmp phosphatase (ec 3.3.3.35) were measured in the epstein-barr virally transformed human b lymphocyte line laz-007. cells were synchronised at different stages of the cell cycle using the technique of centrifugal elutriation. the degree of synchrony in each cycle-stage cell population was determined by flow microfluorimetric analysis of ...	1980	6260157
functional 'immaturity' of isolated b cells from patients with hypogammaglobulinaemia.	the membrane characteristics and in vitro function of the circulating b cells from patients with late-onset hypogammaglobulinaemia (cvid) and from patients with sex-linked hypogammaglobulinaemia (xla) have been investigated using ox red blood cells coated with goat anti-human ig. all seven patients with xla had less than 2.0% smigm + ve cells and attempts to isolate these cells were unsuccessful. the majority of the 27 patients with cvid had greater than 3.0% smigm + ve cells. when cultured eith ...	1980	6260401
infectious mononucleosis and epstein-barr virus in japan. i. clinical and serological aspects.		1980	6261511
non-a, non-b hepatitis.		1980	6261548
variations among isolates of epstein-barr virus.		1980	6261649
epidemiologic aspects of epstein-barr virus (ebv)-associated diseases.		1980	6261650
epstein-barr virus-induced increase in the concanavalin-a receptor density of established ebv-negative lymphoma lines in vitro.	infection of two human ebv-genome negative lymphoma lines with the ebv-variants p3hr-i and b95-8 converts the lines to permanent carriers of ebv-dna and the ebv-determined nuclear antigen. this process is accompanied by a series of biologic changes similar to those in oncogenic transformation by known tumor viruses (e.g. polyoma, sv40, rous sv). they include altered growth properties and membrane changes, one of which is expressed in an increased agglutinability by con-a. although most transform ...	1980	6262102
comparison of in vitro lymphocyte proliferations induced by cytomegalovirus-infected human fibroblasts and cell-free cytomegalovirus.	in vitro lymphocyte reactivity (lr) to cytomegalovirus (cmv)-infected human fetal fibroblasts (cmvff) and cell-free cmv were measured by using lymphocytes from healthy donors. lymphocytes from all seropositive donors were stimulated by cmvff, whereas lymphocytes from negative donors were not. the optimal stimulator cell-to-lymphocyte ratio was in the range of 1:5 to 1:50, dependent on the virus dose used. lr to cell-free cmv was positive for 15 out of 18 seropositive donors and negative for 14 o ...	1980	6262238
on the continuous culturing of b.95-8 cells in the presence of phosphonoacetic acid.	lymphoblastoid b.95-8 cells were cultured for four months and three weeks in the presence of increasing concentrations (50--200 microgram/ml) of phosphonoacetic acid (paa). several weeks after removal of the paa, the cultures, in parallel with untreated b.95-8 cells, were tested for the presence of: 1) epstein-barr virus (ebv) viral capsid antigen (vca), and b) transformation of human cord blood lymphocytes. there was no difference in the percentage of cells exhibiting vca in the b.95-8 paa trea ...	1980	6262340
comparative studies of herpesvirus papio (baboon herpesvirus) dna and epstein-barr virus dna.	an epstein-barr virus (ebv)-like herpesvirus has been isolated from a baboon cell line (594s/f9) by induction with the tumour promoter 12-o-tetradecanoyl-phorbol-13-acetate (tpa). the herpesvirus papio (hvp) dna was mixed with b95 ebv dna and was characterized by sedimentation in neutral glycerol gradient as 55s dna, with a buoyant density of approx. 1.718 g/ml after equilibrium centrifugation in caesium chloride. dna-dna reassociation kinetics between b95 ebv and hvp dna showed that hvp dna sha ...	1980	6262436
effect of actinomycin d and cycloheximide on epstein-barr virus early antigen induction in lymphoblastoid cells.	the effect of inhibitors of rna (actinomycin d, act. d) and protein synthesis (cycloheximide, ch) on induction of epstein-barr virus (ebv) antigens by the tumour promoter tpa and by ebv superinfection has been analysed. the data indicate that (i) concomitant treatment of cells with act. d and tpa leads to complete suppression of virus antigen induction. subsequent treatment of the cells with act. d after prior exposure to tpa results in some virus antigen induction, the amount depending on the t ...	1980	6262437
kaposi's sarcoma and its relationship to cytomegalovirus (cmnv). iii. cmv dna and cmv early antigens in kaposi's sarcoma.	dna-dna reassociation kinetics, anti-complement immunofluorescence (acif) and acif-blocking tests were used to search for cytomegalovirus (cmv) gene products in kaposi's sarcoma (ds) biopsies and early cell cultures deriving from them. three of eight tumor biopsies were positive for cmv dna; two of them at 0.35 genome/cell and one at one copy 25% genome/cell. cmv-related antigens, mainly localized in the nucleus, were found in cryostat sections of seven of 31 tumor biopsies and four of 12 ks cel ...	1980	6263803
changes in the expression of two epstein-barr virus-associated antigens, ebna and rana, during the cell cycle of transformed human b lymphoblasts.	two methods of cell sychronization, density-dependent arrest and double thymidine block, were used to assign two epstein-barr virus-associated antigens to different parts of the growth cycle of the human b lymphblastoid cell lines, wi-l2 and raji. the epstein-barr nuclear antigen (ebna), as detected by anti-complement immunofluorescence, was maximally expressed during early s phase, decreased during the g2 and m phases, and was absent in early g1. in contrast, the rheumatoid arthritis nuclear an ...	1980	6263806
establishment of a lymphoid cell line from leukemic cells of a patient with chronic lymphocytic leukemia.	two lymphoid cell lines were established from a patient with chronic lymphocytic leukemia by infecting blood cells with epstein-barr virus (ebv). studies of morphology, glucose-6-phosphate dehydrogenase, malic enzyme, immunoglobulin, and chromosomes of the two lines indicated that one of them originated from leukemic cells while the other arose from residual normal blood cells. the morphology and capacity for immunoglobulin secretion in the line that arose from leukemic cells were similar to tho ...	1980	6263808
[an outbreak type a hepatitis in a family group living in a village near parma. epidemiologic evaluation and prophylaxis (author's transl)].	the most recent data concerning the characteristics of hepatitis a virus, as well as epidemiology and prophylaxis of type a hepatitis are described. an epidemic of hepatitis a which occurred in a village near parma is analysed. the diagnosis was based on the detection, by radioimmunoassay, of specific antibody to hepatitis a antigen (anti-hav) at the beginning of the infection and during the convalescence. in addition the occurrence of high levels of specific immunoglobulins type m anti hav, dur ...	1980	6263293
effect of retinoic acid (ra) on the epstein-barr virus (ebv)-inducing effect of sodium butyrate.	sodium butyrate is a powerful inducer of the epstein-barr virus (ebv) cycle in the p3hr-1 cell line. retinoic acid (ra) was found to reduce the butyrate induction of early antigen (ea) and viral capsid antigen (vca) by 26-41%. this is similar to the previously reported effect of ra on iudr and tpa induction, with certain quantitative differences between the systems.	1980	6261939
[studies on transformation of human lymphocytes by epstein-barr virus (author's transl)].	a simple method for simultaneous comparative observations of immunofluorescence, cellular morphology and autoradiography has been developed, and this method was applied to investigate the early transformation events of human cord blood lymphocytes by epstein-barr virus (ebv). the results indicated that transformation occurred sequentially from ebv-determined nuclear antigen (ebna) synthesis, to blastogenesis, to dna synthesis, and consistently resulted in unlimited growth of the antigen-positive ...	1980	6263773
molecular theology, immunophilosophy, and autoimmune disease. ii. speculation on the aetiology and pathogenesis of rheumatoid arthritis.		1980	6264588
immunoregulatory functions of cultured human t lymphocytes.	twenty continuous cultures of human t cells (ctc) (15 from normal individuals and five from patients with t cell malignancy) growing in the presence of pha-stimulated lymphocyte conditioned medium were studied for their ability to participate in the regulation of the in vitro immunoglobulin (ig) production induced by five polyclonal activators, pokeweed mitogen (pwm), epstein-barr virus (ebv), nocardia opaca water-soluble mitogen (nwsm), streptolysin o (slo), and staphylococcl phage lysate (spl) ...	1980	6264658
natural antibodies to ebv-vca antigens in common marmosets (callithrix jacchus) and response after ebv inoculation.	healthy common marmosets from two separate colonies, one in england and one in france, were found to have antibodies cross-reacting with epstein-barr virus structural antigens (ebv-vca). all seropositive animals were at least 2 years old. experimental ebv infection of marmosets of different ages led to seroconversion of inoculated weanlings. adult animals either developed antibody for the first time or showed an increase in their existing titers. both control and infected animals developed a pro ...	1980	6265400
quantitative analysis of cell surface hla structures by means of monoclonal antibodies.	quantitative data on the binding of murine monoclonal antibodies ot whole human lymphoblastoid lines and peripheral blood lymphocytes (pbl) are reported. antibodies reacting with beta 2m or a common part of the hla heavy chains and nonpolymorphic determinants of the dr dimer were used. the equilibrium constant (k) of the reaction and the total number of antigenic determinants was graphically estimated. for the above-mentioned antibodies, k ranged between 5 x 10(8) and 4 c 10(9) l/mole at 0 degre ...	1980	6266984
immunoglobulin production in epstein-barr virus-induced human monoclonal lymphoblastoid cell lines.	in order to establish clonal human lymphoblastoid cell lines, human tonsillar lymphocytes infected with epstein-barr virus were directly seeded in semi-solid agar. after four weeks, resulting colonies were randomly isolated and transferred to suspension culture. at around three months after the initiation of the culture, immunoglobulin (ig) production of 17 clones thus established was determined. cytoplasmic and membrane ig were detected by immunofluorescence staining and secretory ig in the cul ...	1980	6266070
investigations of the presence of serum antibodies to epstein-barr virus in patients with various tumors.	serum samples from 150 patients with various malignant or benign tumors and from 20 clinically healthy subjects were tested by indirect immunofluorescence (if) reaction for the presence of specific antibodies to epstein-barr-virus-determined viral capsid antigen (eb vca). the if test-carried out in multitest-slide eb3 and raji cell preparations-revealed antibodies to eb vca in 102 of the patients with tumors and in 4 of the healthy persons.	1980	6266130
burkitt's lymphoma cell line bearing surface iga and negative for nuclear antigen of epstein-barr virus (ebna).	a new continuous cell line, tl-1, was established in tissue culture from burkitt's lymphoma arising at the ileocecal region. it has been maintained for the past two years. the cells were small and round, and grew in a suspension culture. population doubling time was approximately 36 hours, the cells revealed the chromosomal abnormality i.e. translocation between 8 and 14 chromosomes, which was reported on some burkitt's lymphomas. this line was negative for nuclear antigen of eb virus (ebna) and ...	1980	6268866
re-evaluation of a transforming strain of epstein-barr virus from the burkitt lymphoma cell line, jijoye.	the biological properties of epstein-barr virus (ebv) from a burkitt lymphoma cell line, jijoye, were examined. the synthesis of virus capsid antigen (vca) and early antigen (ea) in jijoye cells was markedly enhanced by shift-down of the temperature of incubation from 37 degrees c to 33 degrees c. cultures of jijoye cells at 33 degrees c released a large amount of transforming ebv (10(5.2) of 50% transforming doses/ml) into the culture fluid. however, no infectious virus was produced in all cult ...	1980	6270005
multiplicity-dependent biological and biochemical properties of epstein-barr virus (ebv) rescued from non-producer lines after superinfection with p3hr-1 ebv.	superinfection of lymphoblastoid cells of ebv non-producer lines with non-transforming p3hr-1 ebv leads to the rescue of transforming virus. at least part of the recovered molecules represent recombinant dna between superinfecting p3hr-1 ebv and resident viral genomes (fresen et al., 1979, 1980). with high titer stocks of superinfecting p3hr-1 ebv, viral particles with early antigen (ea)-inducing properties can be rescued, indicating that under these conditions of infection input viral genomes m ...	1980	6270006
induction of the ebv cycle in b-lymphocyte-derived lines is accompanied by increased natural killer (nk) sensitivity and the expression of ebv-related antigen(s) detected by the adcc reaction.	human b-lymphocyte-derived lines were forced to enter the ebv-cycle by superinfection with the p3hr-1 substrain of ebv or sodium butyrate treatment. the induced cells were used as targets for natural killing (nk) and ebv-specific, antibody-dependent cellular cytotoxicity (adcc). two burkitt lymphoma lines, raji and daudi, and one normal adult derived lymphoblastoid cell line, nad-7, were comparable in their adcc-sensitivity after induction, but only the burkitt lymphoma-derived lines showed a ma ...	1980	6270007
[application of anticomplement immunoenzymatic method for the detection of ebna in carcinoma cells and normal epithelial cells from nasopharynx (author's transl)].		1980	6273001
[antibodies to eb viral capsid antigen in rhesus monkey sera (author's transl)].		1980	6273003
a case of malignant lymphoma, undifferentiated, burkitt's type with high antibodies to epstein-barr virus.	a 5-year-4-month-old girl was admitted to taipei municipal jen-ai hospital because of a rapidly enlarging right submandibular tumor. the diagnosis of malignant lymphoma, undifferentiated, burkitt's type was made by distinctive histopathological features. positive serological findings related to epstein-barr virus infection were disclosed. complete remission has been achieved since the treatment with high-dose cyclophosphamide was given, and no signs of relapse have been found yet.	1980	6265160
herpesvirus saimiri: protective effect of attenuated strain against lymphoma induction.	an attenuated strain of herpesvirus saimiri (a-hvs) induced a persistent, latent infection in common marmosets. virus was recovered from circulating lymphocytes of infected animals and antibody response to virus-specified antigens was demonstrated over 3-12 months pi. a-hvs-infected common marmosets and animals without previous a-hvs exposure were challenged with oncogenic hvs (125-825 pfu); marmosets without a-hvs infection developed malignant lymphoproliferative disease and died 3 weeks pi whe ...	1980	6265382
colony morphology, cell growth and viral radiosensitivity as parameters for detecting strain differences in epstein-barr virus (ebv).	several parameters of two lymphocyte-transforming epstein-barr viruses (ebvs), b95-8 and olare, derived from infectious mononucleosis (im) and african burkitt's lymphoma (bl), respectively, were compared to determine if there were biological differences between epstein-barr viral strains. morphology of transformed colonies, growth of transformed cell lines, sensitivity to uv light and x-irradiation, as well as ebv-related, antigens were investigated in autochthonous cell lines. the morphology of ...	1980	6265384
epstein-barr virus infection in children entering a paediatric unit.		1980	6309968
chromosome 14q+ in a japanese patient with burkitt's lymphoma.	cytogenetic studies were performed on a biopsy specimen of a jaw tumor and on a bone marrow aspirate from a japanese patient with epstein-barr virus-negative burkitt's lymphoma. a 14q + chromosome was found in cells from either source, although each contained a different clone. other karyotypic abnormalities present in common included 2dir dup (1q) (q21 leads to q32), 3q+, 6p--, +12, +mar.	1980	6446841
multiple hepatitis attacks in drug addicts.	radioimmunoassays for detection of hepatitis b surface antigen (hbsag), antibody to hbsag (anti-hbs), antibody to hepatitis b core antigen (anti-hbc), antibody to hepatitis a virus (anti-hav), and anti-hav of igm class were used to verify hepatitis a and hepatitis b infection in 33 drug addicts with multiple attacks of hepatitis. hepatitis a was confirmed serologically in 23 (32%) of 71 total hepatitis episodes, while hepatitis b was confirmed in 30 episodes (42%). the remaining 18 hepatitis epi ...	1980	6766507
interaction of herpesvirus ateles and hepesvirus saimiri with primate lymphocytes. i. selective adsorption of virus by lymphoid cells.	a semiquantitative infectious bioassay, described by sairenji and hinuma for the measurement of epstein-barr virus (ebv) receptors, was adapted to study the adsorption of the lymphotropic new world herpesvirus ateles (hva) to established lymphoid lines and lymphocytes of human versus marmoset origin. all marmoset t-cell lines [hva- or hvs (herpesvirus saimiri)-carrying] had a high adsorbing capacity. ebv-carrying marmoset or human b-cell lines, one ebv-negative human b-lymphoma line, and one hum ...	1980	6766914
etiological characterization of hepatitis b surface antigen-negative hepatitis among adult patients in athens, greece.	in a 4-month period, 216 cases of acute viral hepatitis were diagnosed in adults at the infectious diseases hospital, athens, greece. twenty-six percent of these were hepatitis b surface antigen negative. a full set of clinical specimens was obtained from 19 of these patients, who were studied in depth for the etiology of their hepatitis. a total of 7 of the 19 patients had serological evidence of hepatitis a virus infection, and 2 had evidence of recent hepatitis b virus infection. the remainin ...	1980	6769949
[viral hepatitis. occupational hazard for anaesthesists (author's transl)].	hepatitis has been recognized as an occupational hazard of health professionals for many years. the recent findings concerning the structure of hepatitis a, b, non-a non-b, as well as refined testing procedures have increased the ability and thus the frequency of its diagnosis in occupationally exposed medical personnel. other viruses such as epstein barr virus (ebv) and cytomegalovirus (cmv) can also be involved. among the inferences to be drawn from recent research is that hepatitis b virus ha ...	1980	6775557
prevalence of type b and non-a, non-b hepatitis in hemophilia: relationship to chronic liver disease.	to assess the relationship of liver dysfunction and hepatitis markers in hemophilic patients treated with factor viii or ix concentrates, we studied 103 patients with hemophilia a and b for 6-36 mo. elevated serum alanine aminotransferase was noted in 79% of the patients, with 51% of the patients showing persistent elevation for longer than 6 mo. thirteen patients (12%) were hbsag-positive, with 8 patients showing persistence of hbsag and abnormal serum alanine aminotransferase for more than 6 m ...	1980	6777232
a non-a non-b hepatitis epidemic in a hb antigen-free haemodialysis unit. demonstration of serological markers of non-a non-b virus.	one hundred and thirty six patients receiving haemodialysis in a hb antigen-free unit were prospectively studied over a period of 29 months for evidence of hepatitis. twelve/one hundred and eleven patients who were dialysed in this unit for at least one month developed elevation of alt which proved to be related to neither toxic hepatitis nor hepatitis due to any of the following viruses: hepatitis b virus (hbv), hepatitis a virus (hav), cytomegalovirus (cmv) or epstein-barr virus (ebv). therefo ...	1980	6787582
hepatitis non-a, non-b.	evidence for the existence of hepatitis non-a, non-b includes epidemiologic data and results of transmission studies indicating the presence of hepatitis that could not be explained by known causative agents. the diagnosis is suggested in patients who have multiple episodes of acute hepatitis or who contract hepatitis after transfusion, hemodialysis or drug abuse. sporadic cases are common. three such cases are described to illustrate that the disease is clinically indistinguishable from hepatit ...	1980	6790149
transplantability of human lymphoid cell line, lymphoma, and leukemia in splenectomized and/or irradiated nude mice.	the effects of splenectomy and/or whole-body irradiation of nude mice before xenotransplantation of lymphoid cell lines, lymphoma, and leukemia were studied. transplantation after whole-body irradiation resulted in the increased "take" rate of three cultured cell lines (two of t-cell-derived acute lymphocytic leukemia and one of b-cell derived acute lymphocytic leukemia) and in the tumorous growth of burkitt-derived raji and spontaneously transformed lymphoblastoid cell lines. with splenectomy p ...	1980	6930325
establishment and characterization of a human acute monocytic leukemia cell line (thp-1).	a human leukemic cell line (thp-1) cultured from the blood of a boy with acute monocytic leukemia is described. this cell line had fc and c3b receptors, but no surface or cytoplasmic immunoglobulins. hla haplotypes of thp-1 were hla-a2, -a9, -b5, -drw1 and -drw2. the monocytic nature of the cell line was characterized by: (1) the presence of alpha-naphthyl butyrate esterase activities which could be inhibited by naf; (2) lysozyme production; (3) the phagocytosis of latex particles and sensitized ...	1980	6970727
establishment of autoantibody-producing cell lines from peripheral blood lymphocytes of patients with systemic lupus erythematosus.	autoantibody-producing b cell lines were established from peripheral blood lymphocytes of patients with systemic lupus erythematosus. peripheral blood lymphocytes obtained from five of seven patients were successfully transformed by epstein-barr virus. two of four established b lymphoblastoid cell lines examined in this study produced anti-nuclear factor antibodies and one of them produced anti-single-stranded dna and anti-double-stranded dna antibodies. these results indicate that b cell clones ...	1980	6971392
infections in leukemic children: a prospective analysis.	a 28-month prospective study of 54 leukemic children was carried out to determine the incidence and type of infection associated with febrile episodes. fever was caused by infections in 84 of 199 episodes (71%). two-thirds of the febrile episodes and 57% of the documented infections occurred when leukemic activity was demonstrable. however, only nine of 29 febrile episodes which occurred at the time of initial diagnosis of acute leukemia were due to infection. all serious bacterial infections oc ...	1980	6988557
t lymphocytes expressing human ia-like antigens in infectious mononucleosis (im).	in six patients with epstein-barr virus (ebv) induced infectious mononucleosis (im) and in two patients with im-like syndrome, peripheral blood lymphocytes in the acute and convalescent phase were tested for human la-like antigens as well as other cell surface markers. the major population of t lymphocytes in the acute phase showed la-like antigens, as detected by indirect immunofluorescence with heteroantisera, and the number of la-like antigen-positive t lymphocytes decreased with convalescenc ...	1980	6996766
primary epstein-barr virus infection associated with a rejection episode in a human renal allograft recipient.	five weeks after receiving a kidney donated by his brother, a 33 year old man showed signs of graft rejection concurrent with an epstein-barr virus infection. primary infection with this virus has not been previousy documented in a renal transplant recipient and the possibility of a relationship between the infection and the kidney rejection is discussed.	1980	6996877
chromosome aberrations acquired in vitro by human b-cell lines. ii. distribution of break points.	among 165 human b-cell lines examined, 57 were found to have karyotypic abnormalities that involved chromosome breakage. the sites of breakage were identified with quinacrine-, giemsa-, and reverse-banding techniques, and the distribution of 239 break points was plotted. a pronounced excess of telomeric and, to a lesser extent, centromeric breaks was observed. chromosomes no. 7, 8, 9, 11, and 14 were involved in structural rearrangements more often and chromosomes no. 2, 5, 10, 20, and x less of ...	1980	6967123
involvement of the b-lymphoid system in chronic myelogenous leukaemia.	studies with glucose-6-phosphate dehydrogenase (g6pd) isoenzymes have demonstrated that chronic myelogenous leukaemia (cml) is a clonal disorder of pluripotent haematopoietic stem cells which are capable of differentiation to myeloid cells, monocytes, erthrocytes and platelets. it has been observed recently in g6pd heterozygous patients with chronic phase cml that the non-e-rosetting lymphocytes were restricted to a single enzyme type, indicating that some lymphoid cells must also arise from the ...	1980	6968038
nonrandom chromosomal aberrations in chronic lymphocytic leukemia revealed by polyclonal b-cell-mitogen stimulation.	peripheral blood lymphocytes from 11 patients with chronic lymphocytic leukemia were stimulated by epstein-barr virus (ebv), lipopolysaccharide from escherichia (lps), and phytohemagglutinin (pha). chromosome analysis with the q-banding technique after 5 days incubation revealed an extra chromosome 12 in 5 of the patients and a translocation between chromosome 11 and chromosome 14 in 1. two patients had a deletion of chromosome 6, and only 3 patients had a normal karyotype. in most patients, the ...	1980	6968231
polyclonal b-cell lymphoma during infection with epstein-barr virus.		1980	6968402
x-linked lymphoproliferative syndrome registry report.	immune deficiency, especially to the epstein-barr virus, and increased susceptibility to fatal infectious mononucleosis, acquired agammoglobulinemia, and lymphoma are the cardinal features of the x-linked lymphoproliferative syndrome. since the establishment of the xlp registry in september, 1978, 59 affected males in seven unrelated kindreds were comprehensively studied. a spectrum of lymphoproliferative phenotypes was observed. thirty-four patients (57%) died from infectious mononucleosis, eig ...	1980	7188959
diethylstilbestrol-exposed monozygotic twins discordant for cervicovaginal clear cell adenocarcinoma.	monozygotic, 17-year-old, female twins exposed to diethylstilbestrol (des) in utero and discordant for cervicovaginal clear cell adenocarcinoma are reported. benign teratologic changes were present in both. the twin with cancer was treated by extensive operation and postoperative irradiation. no tumor has appeared in either twin in the 4 years subsequent to therapy. discordance for cervicovaginal clear cell adenocarcinoma in this situation suggests that factors other than embryonic exposure to d ...	1980	7189642
nasopharyngeal carcinoma: epidemiology, histopathology and aetiology.	the highest incidence of nasopharyngeal carcinoma (around 20 per 100,000 per year) is found in chinese populations in many countries. elevated rates (5--15) are found in eskimos, and in several racial groups in south-east asia. the incidence rates in malta and in some parts of northern africa are also moderately elevated. the incidence rates are low (less than 1) in virtually all other parts of the world. in singapore, the incidence rates are high in chinese (18.4), intermediate in malays (4.7) ...	1980	7212609
viruses and cancer risk in man.	the present state of tumor virus research in man is analysed in order to evaluate the cancer risk by viruses in man. for most of the viruses isolated from human tumorous or normal tissues and similar to oncogenic viruses of animals the participation as causative agent in the multifactorial process of cancer induction, promotion and realization in man is not yet established. this holds true for dna viruses (paillomaviruses, sv 40-related viruses, herpesvirus 2, hepatitis b virus) as well as for t ...	1980	7224804
acute cerebellar ataxia associated with epstein-barr virus infection.		1980	6892515
chromosome analyses of lymphoid cell lines derived from patients with chronic lymphocytic leukemia.	to study chromosome complements of chronic lymphocytic leukemia cells, six epstein-barr virus-transformed lymphoid lines were established from two patients with this disease who were heterozygous for the x-chromosome-linked enzyme glucose-6-phosphate dehydrogenase (g6pd). immunoglobulin and g6pd were used as markers of the leukemic versus normal cell origin of the cell lines. the two lines, derived from putative normal cells, had no chromosomal changes. in contrast, chomosome abnormalities were ...	1980	7239716
[burkitt lymphoma of african type in europe (author's transl)].	a 34-year-old german woman who had never been to africa developed a burkitt lymphoma of african type during the fifth month of pregnancy. the diagnosis was confirmed during life by cytological and virological tests. there was a markedly increased antibody titre against the epstein-barr virus in serum as well as virus antigen in the tumour tissue. there was extensive involvement of almost all abdominal organs and of the breast. after spontaneous abortion of a female foetus in the sixth month of p ...	1980	7363786
[burkitt's lymphoma with leukemic evolution].	an account of the clinical, histopathological and aetiopathogenetic aspects of burkitt's lymphoma is followed by the presentation of a personal case, in which the typical clinical picture of the african form progressed to terminal leukaemia. the aetiological and pathogenetic rôle of the epstein-barr virus is also briefly discussed.	1980	7383398
production of predominantly polymeric iga by human peripheral blood lymphocytes stimulated in vitro with mitogens.	human peripheral blood lymphocytes (pbl) were cultured for various time periods (up to 8 d) in the presence of pokeweed mitogen (pwm), lipopolysaccharide, or epstein-barr virus. cell-free supernates were fractionated on a standardized ultrogel aca 22 column and the proportion of polymeric and monomeric iga was determined by radioimmunoassay. the results demonstrate that pbl stimulated with these mitogens produce igm and igg with molecular characteristics identical to those found in serum, but th ...	1980	7430951
[agranulocytosis in the course of infectious mononucleosis. demonstration of leukoagglutinins (author's transl)].	a case of agranulocytosis, quickly and spontaneously reversible, occurring in the fourth week of infectious mononucleosis in a 7-year old girl, is reported. the interest of the case lies in the importance of the symptomatology (jaundice, eruption, fever for 40 days), the serological detection of the role of the epstein-barr virus and, in particular, in the presence of leukoagglutinins judged to be responsible for the agranulocytosis.	1980	7469685
a fatal case of epstein-barr virus infection with jaundice and renal failure.	a fatal case of epstein-barr virus infection in a 17-year-old male is described. the patient presented with an illness clinically typical of infectious mononucleosis but death followed development of renal failure, jaundice and pulmonary failure. there was no absolute lymphocytosis nor a significant number of atypical mononuclear cells in his peripheral blood. however, heterophile antibody and epstein-barr virus-specific igm were present.	1980	7267486
human lymphoblastoid cell line producing specific antibody against rh-antigen d.	a lymphoblastoid cell line producing specific anti-rh antibody against d determinant was established by epstein-barr virus (ebv) infection. the lymphocytes were from an immunized male donor with high titre of anti-d antibody. the cells were preselected by rosetting them with rh-positive erythrocytes before ebv infection. the cell line produced specific antibody of igg class, namely igg1 subclass, but it also produced nonspecific igm and igg. by rerosetting the cell line, the specific antibody-pr ...	1980	9537031
epstein-barr virus-induced lymphoblastoid cell lines from patients with primary immunodeficiency diseases.	peripheral lymphocytes from eight patients with congenital immunodeficiency diseases were infected with epstein-barr virus (ebv) in an attempt to establish b lymphoblastoid cell lines (lcl). these patients included three boys with congenital agammaglobulinaemia, two girls with hypogammaglobulinaemia, one boy with common variable immunodeficiency, one boy with severe combined immunodeficiency with adenosine deaminase deficiency, and one boy with digeorge syndrome. five of the patients bore no sur ...	1980	9537041
spontaneous cell-mediated cytotoxicity (scmc) in various mammalian species and chickens: selective reaction pattern and different mechanisms.	cultured cell lines derived from donors of various species served as 75se- or 51cr-labelled targets in microcytotoxicity assays. as in human donors, considerable spontaneous cell-mediated cytotoxicity (scmc) was exhibited by peripheral blood lymphoid effector cells from healthy chickens, mink, swine, cattle, horses and tigers. although all target lines tested could be lysed in scmc, there was no 'general scmc target' for all species or all individuals of one species. the selectivity, the kinetic ...	1980	9537048
histocompatibility antigens and humoral immunity to epstein-barr virus.	to explore possible genetic determinants of immunity to epstein-barr (eb) virus infection, prevalence rates and geometric mean titers (gmt) of antibody against eb viral capsid antigen (vca) were measured in 422 adults of known hla and abo histocompatibility types. deviation from the overall prevalence (87%) of anti-vca titers > 10 was limited to subjects with hla-a10 (95%) and those with a "blank" hla-a locus (78%). the latter deviation was largely accounted for by the 13 subjects of hla-a1/blan ...	1980	12735311
structural polypeptides of choristoneura biennis entomopoxvirus.	the structural polypeptides of choristoneura biennis (free) entomopoxvirus (epv) were analyzed. the virion contained at least 40 polypeptides ranging from 12,000 to 250,000 in molecular weight. twelve major proteins constitute 95% of the total virus protein. core particles generated by treatment with nonidet p-40, under conditions during which endogenous alkaline protease is active, display a smooth exterior similar to those obtained for orthopoxviruses. the major component of these cores is a p ...	1980	18631663
initiation points for cellular deoxyribonucleic acid replication in human lymphoid cells converted by epstein-barr virus.	replicon size was estimated in two epstein-barr virus (ebv)-negative human lymphoma lines, bjab and ramos, and four ebv-positive lines derived from the former ones by infection (conversion) with two viral strains, b95-8 and p3hr-1. logarithmic cultures were pulse-labeled with [3h]thymidine, and the deoxyribonucleic acid was spread on microscopic slides and autoradiographed by the method of huberman and riggs. after developing, replication forks were visualized as silver grain tracks on the autor ...	1981	9279388
no activation of new initiation points for deoxyribonucleic acid replication in balb/c 3t3 cells transformed by kirsten sarcoma virus.	balb/c 3t3 cells were transformed by kirsten sarcoma virus, and five clones were isolated in soft agar. average replicon sizes of the transformed cell lines were estimated by the method of fiber-autoradiography (j. a. huberman and a. d. riggs, j. mol. biol.32:327-341, 1968) and found to be the same size as the nontransformed 3t3 cells, analyzed in parallel. the results indicate that, unlike simian virus 40 and epstein-barr virus, kirsten sarcoma virus does not activate new initiation points for ...	1981	9279389
striking similarities are exhibited by two small epstein-barr virus-encoded ribonucleic acids and the adenovirus-associated ribonucleic acids vai and vaii.	the nucleotide sequence of the region of the epstein-barr virus genome that specifies two small ribonucleic acids (rnas), eber 1 and eber 2, has been determined. both of these rnas are encoded by the right-hand 1,000 base pairs of the ecori j fragment of ebv deoxyribonucleic acid. eber 1 is 166 (167) nucleotides long and eber 2 is 172 +/- 1 nucleotides long; the heterogeneity resides at the 3' termini. the eber genes are separated by 161 base pairs and are transcribed from the same deoxyribonucl ...	1981	9279391
[gianotti-crosti acropapulo-vesicular syndrome in a primary infection caused by epstein-barr virus].		1981	7290959
detection of specific iga antibodies in serum of patients with varicella and zoster infections.	a sensitive solid-phase, enzyme-linked immunosorbent assay (elisa) was developed for detection of serum iga antibodies to varicella-zoster virus (vzv). the antigen consisted of a sonically disrupted extract of vzv-infected human embryo cells. rabbit antihuman iga peroxidase conjugate was used to detect human iga bound to viral antigens. in parallel, igm and igg antibodies to vzv were studied by elisa and by an immunoperoxidase antibody to membrane antigen technique, respectively. vzv iga antibod ...	1981	7298297
intrafamilial transmission of epstein-barr virus infections.	intrafamilial transmission of epstein-barr virus infections was studied in 35 familial. after infectious mononucleosis was diagnosed in an index child, all siblings were tested for antibodies to ebv. susceptible children were retested in four to eight weeks. in five families, a concurrent seroconversion was detected in an asymptomatic younger child. two of 35 seronegative siblings developed clinical im, confirmed serologically, during the study period. overall, there evidence of spread in 7 (20% ...	1981	7452392
interactions between human lymphoblastoid cells and human fibroblast feeder layers in vitro.	lymphoblastoid (lb) cells interact in vitro with human fibroblasts. cell complexes can be dissociated by trypsin treatment. binding on fibroblasts is higher for epstein-barr virus-producer lb cells than for nonproducers, and depends on the origin of the fibroblastic cells. stromal fibroblasts isolated from a metastatic lymph node tumor exhibited a highly increased potency of binding lb cells in comparison with fibroblasts isolated from normal breast epidermis or the primary breast tumor from the ...	1981	7250480
cytogenetic studies on african burkitt's lymphoma cell lines: t(8;14), t(2;8) and t(8;22) translocations.	cytogenetic studies on ten african epstein-barr virus (ebv) positive burkitt's lymphoma (bl) cell lines were performed. the usual translocation t(8;14) (q24;q32) was found in five of them, a deletion del(8) (q24 leads to qter) in another one, while four variants were observed, two of these having a t(2;8) (p12;q24) translocation and two a t(8;22) (q24;q11) translocation. other chromosome abnormalities were seen in seven of the cell lines, but these varied from one cell line to another. thus, var ...	1981	7260888
x-linked lymphoproliferative syndrome. an immunodeficiency disorder with acquired agammaglobulinemia, fatal infectious mononucleosis, or malignant lymphoma.	the x-linked lymphoproliferative syndrome (xlp) is characterized by a combined variable immunodeficiency with vulnerability to epstein-barr virus (ebv)-induced fatal or chronic infectious mononucleosis, acquired agammaglobulinemia, aplastic anemia, or malignant b cell lymphomas. diagnosis of xlp requires documentation of two or more maternally related males with these phenotypes. epstein-barr virus must be demonstrated in circulating blood, lymphoid tissues, or saliva of infected males. characte ...	1981	6894075
fatal reaction to dapsone during treatment of leprosy.	a burmese boy being treated with dapsone (diaminodiphenylsulfone [dds]), 100 mg daily, for lepromatous leprosy had a fatal reaction to the drug 3 weeks after therapy was started. the clinical symptoms and progression of illness conform well to a "dds syndrome" first described in the early 1950s. although the syndrome clinically resembles infectious mononucleosis, neither epstein-barr virus nor cytomegalovirus was implicated as an etiologic agent in this case. the syndrome has been recognized dur ...	1981	7235421
phylogeny of nk cell reactivity against human and nonhuman primate lymphoblastoid cell lines: evolving and conserved target antigens.	both human and nonhuman primate natural killer (nk) cells display little or no killing against allogeneic b lymphoblastoid cell lines. however, the same b cell lines are killed in baboon-human (b alpha h) or human-baboon (h alpha b) xenogeneic combinations. competition-inhibition experiments indicate that the xenogeneic determinants recognized by nk cells are found principally if not exclusively on b rather than t target cells. cell lines from closely related chimpanzee or orang-utan species can ...	1981	6970228
[fatal primary epstein-barr virus infections with or without infectious mononucleosis (author's transl)].		1981	7013603