Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| entamoeba histolytica: cytopathogenicity and lectin activity of avirulent mutants. | three clones of entamoeba histolytica (l-6, c93, c919) were isolated by mutagenesis with ethyl methanesulfonate from the axenic strain hm1:imss and were studied for adherence, cytolytic, and soluble galactose inhibitable lectin activity. avirulent clones adhered to and killed fewer chinese hamster ovary cells than hm1:imss (p less than 0.01). however, only c919 was deficient in adherence to red blood cells. galactose (1.0 g) completely inhibited adherence of all the mutants to chinese hamster ov ... | 1987 | 2883020 |
| indications for treatment. | 1987 | 2884194 | |
| inhibition of the adhesion of entamoeba histolytica trophozoites to human erythrocytes by carbohydrates. | we have analyzed the effect of 14 carbohydrates (seven monosaccharides, four disaccharides and three aminosugars) on the adhesion of entamoeba histolytica hk9 trophozoites to human red blood cells (rbc). amebal adhesion was significantly inhibited by five of these carbohydrates with the following order of potency: lactose (lac) greater than n-acetylgalactosamine (galnac) greater than melibiose (mel) greater than galactose (gal) greater than n-acetylglucosamine (glcnac). the mean inhibitory conce ... | 1987 | 2894024 |
| antiamoebic activity of chonemorphine, a steroidal alkaloid, in experimental models. | chonemorphine, a steroidal alkaloid isolated from chonemorpha fragrans moon (apocyanaceae) was identified as an antimoebic principle during the course of a screening programme for novel antiparasitic agents from plant sources. at a dosage of 100 mg/kg x 4 chonemorphine led to a 100% cure of experimental hepatic infection in golden hamsters and cleared 90% of the intestinal infection in weanling wistar rats at 200 mg/kg (x4) dosages. the discovery of chonemorphine as an antiamoebic agent is an ad ... | 1987 | 2894025 |
| acid phosphomonoesterase heterogeneity in axenically grown entamoeba histolytica. | 1987 | 2895609 | |
| amoebicidal activity of nitrovinylindole. | 1987 | 2897332 | |
| [pseudomembranous colitis after antibiotic therapy associated with the presence of entamoeba histolytica histolytica. apropos of 2 cases]. | the authors report on two cases of pseudomembranous colitis (p.m.c.) developed in two senegalese women of 38 and 36 years, and discovered at the 4th and 5th day respectively of an antibiotherapy based on ampicillin. in these two observations, cysts of entamoeba histolytica histolytica were found in both feces and biopsies. they recall the circumstances of the occurrence, diagnosis techniques and treatment. they underline the unfrequency of this disease in africa south of sahara and they discuss ... | 1987 | 2893239 |
| catalytic classes of proteinases of entamoeba histolytica. | endopeptidase inhibitors were used to determine the catalytic classes of proteinases present in extracts of entamoeba histolytica (strain hm 1:imss) axenically grown in vitro. cysteine proteinases account for most of the proteolytic activity; one or more proteinases with different catalytic mechanisms are also present but could not be unambiguously assigned to a particular catalytic class. proteinases in amebic lysates were resolved by polyacrylamide gel electrophoresis with sodium dodecyl sulfa ... | 1987 | 2893258 |
| hla antigens associated to amoebic abscess of the liver in mexican mestizos. | worldwide prevalence of amoebiasis is estimated at 4 x 10(8) cases/year, yet only one of about 300 individuals harbouring entamoeba histolytica suffers tissue invasion and these cases are mostly concentrated in certain areas of asia, africa and latin america. patients with amoebic abscess of the liver (aal) represent only a small fraction of that. these contrasting figures have been tentatively explained on the one hand through variations in sex, immunocompetence, nutritional and other socioecon ... | 1987 | 2893327 |
| amoebic peritonitis in pregnancy in the united kingdom. | we describe a woman who developed hepatic amoebic abscesses during pregnancy, and subsequently amoebic peritonitis. we believe this to be the first such recorded case in the united kingdom, and unusual in that the patient had never been abroad. | 1987 | 2893366 |
| molecular weight analysis of entamoeba histolytica antigens recognized by igg and igm antibodies in the sera of patients with amoebiasis. | the sera of four patients with amoebic liver abscess and two patients with diarrhea caused by entamoeba histolytica were tested on "western blots" prepared from e. histolytica strains a3, sfl-3 and hk-9 separated by sds-page. igg antibodies in the sera from patients with liver abscess were found to react with several "major" (i.e. strongly staining) band of approximate molecular weights 150-250 kd, 100 kd, 80 kd, 60 kd, 35 kd, 30 kd, 20 kd, and 5-15 kd and the 150-250 kd group of bands. a compar ... | 1987 | 2893443 |
| course of entamoeba histolytica infection in nude mice. | the course of intestinal infection by entamoeba histolytica was examined in nu/nu balb/c mice. mice were inoculated intracecally with 2.5 x 10(5) polyxenic trophozoites and killed at various times after injection. there was no significant difference in the number of e. histolytica trophozoites recovered in the ceca of nu/+ and t cell-deficient nu/nu mice for the first 25 days after amebic injection. thirty days following amebic injection the number of trophozoites recovered in the ceca of nu/nu ... | 1987 | 2893444 |
| isoenzyme patterns of entamoeba histolytica isolated from homosexual men. | this is a preliminary report on isoenzyme patterns of entamoeba histolytica which were examined by starch gel electrophoresis. by this means the amoebae isolated from 17 asymptomatic male homosexuals were characterized into zymodemes. no amoebic strain isolated corresponded to a pathogenic zymodeme. | 1987 | 2894714 |
| immunological characterization of axenic entamoeba histolytica related antigens. | 1987 | 2895066 | |
| plant based antiamoebic drugs; part i. antiamoebic activity of phenanthroindolizidine alkaloids; common structural determinants of activity with emetine. | 1987 | 2895482 | |
| entamoeba histolytica in inbred mice: evidence of liver involvement. | following observations that certain mouse strains appeared to be susceptible to liver invasion by entamoeba histolytica, mouse strains cba/hn, cba/hn, nu/nu, cba/hn nu/+, cba/hn dh/+, cba/hn +/+, c3h/hej lps/lps and ab/h, were infected intra-intestinally with strain saw 408 (zymodeme ii) of the amoeba. a number of mice from each group died during the first 14 days with acute lesions. the remainder were killed at 4 and 6 months after infection. cba/hn, cba/hn dh/+, and cba/hn +/+ mice all had amo ... | 1987 | 2895511 |
| the effect of orally administered entamoeba histolytica on wistar and athymic (rnu rnu) rats observed during a 12-month period. | groups of lacp (wistar) and rnu rnu athymic rats were dosed orally with cultured trophozoites of 2 strains of entamoeba histolytica, zymodeme ii, and observed for up to 12 months. one group of wistar rats remained infected but with no apparent disease for 6 months; faecal samples from another became negative after one week. all the athymic rats were still infected at 12 months and amoebae could be cultured from their stools. the effect of even long-term infection was usually only mild erosion of ... | 1987 | 2895512 |
| immunocytochemical detection of entamoeba histolytica. | human anti-entamoeba histolytica immunoglobulin was used to detect entamoeba histolytica in 74 positive samples from several different sources, using an indirect immunoperoxidase method. in 73 samples, the protozoan was easily identified. trophozoites and cysts of all cultured entamoeba strains examined were strongly stained, and as few as 3 trophozoites per microscope slide could be detected. in addition, 51 negative control samples were also tested and non-specific reactions were not observed. ... | 1987 | 2895513 |
| an enzyme-linked immunosorbent assay for the detection of entamoeba histolytica antigens in faecal material. | this paper describes a method for the detection of entamoeba histolytica antigens in stool samples using a multi-layer elisa. the method is sensitive and specific, showing no interference with other intestinal parasites, e.g. e. coli, e. hartmanni, endolimax nana, iodamoeba buetschlii, hymenolepis nana, giardia lamblia, trichomonas and ascaris. the method provides a rapid and simple screening assay for e. histolytica infections and should assist in diagnosis and epidemiological studies of the di ... | 1987 | 2895514 |
| seroepidemiologic study of giardiasis patients and high-risk groups in a midwestern city in the united states. | serum antibodies to giardia lamblia were measured in giardiasis patients, in groups at high risk for intestinal parasite infection, and in controls by an indirect fluorescent antibody (ifa) technique. symptomatic patients had the highest antibody titers, and antibodies remained present for up to 18 months in persons with chronic infection. indochinese refugees and male homosexuals with the acquired immunodeficiency syndrome (aids) and pre-aids had higher mean antibody levels than did healthy con ... | 1987 | 2886069 |
| the role of gamma interferon in the generation of human macrophages cytotoxic for entamoeba histolytica trophozoites. | upon exposure to entamoeba histolytica antigen, lymphocytes from patients treated for amebic liver abscess produce lymphokines which activate monocyte-derived macrophages to kill e. histolytica trophozoites. we now demonstrate that gamma interferon (ifn-gamma) is produced by these stimulated lymphocytes and is sufficient but not exclusively necessary to activate monocyte-derived macrophage amebicidal activity. supernatants from mononuclear cells of 7 patients when stimulated with amebic antigen ... | 1987 | 2886071 |
| evidence for two mechanisms of human erythrocyte endocytosis by entamoeba histolytica-like amoebae (laredo strain). | a study of the ultrastructural aspects of endocytosis of human erythrocytes by entamoeba histolytica-like amoebae (laredo) revealed two different mechanisms of endocytosis. first, there is classical phagocytosis which consists of the formation of a phagocytotic pseudopod. this process begins with the engulfment of the red blood cell followed by its entrapment in a food vacuole of the same size as the erythrocyte. it is then digested in the food vacuole. the second means of endocytosis is achieve ... | 1987 | 2886165 |
| entamoeba histolytica: chemoattractant activity for gerbil neutrophils in vivo and in vitro. | the kinetics of the host's cellular response in the peritoneal cavity of gerbils toward axenic pathogenic and nonpathogenic entamoeba histolytica strains were examined. amebae contained in diffusion chambers or free in the peritoneum elicited a neutrophilic response accompanied by decreased levels of macrophages and lymphocytes. pathogenic amebae (ip:0682:1 strain) elicited a neutrophilic response greater than the nonpathogenic dkb and "entamoeba-like" laredo amebae. the neutrophil eliciting fac ... | 1987 | 2886359 |
| cytopathogenicity of entamoeba histolytica: the role of amebic adherence and contact-dependent cytolysis in pathogenesis. | the mechanisms by which entamoeba histolytica trophozoites adhere to and lyse target cells are reviewed from the perspective of pathogenesis. adherence via the galactose and n-acetyl-galactosamine inhibitable amebic lectin and possible additional amebic adhesin molecules is followed by target cell death. inhibition of the gal/galnac lectin with galnac inhibits amebic cytolysis of target cells. amebic activities implicated in the cytolytic event include vesicle exocytosis and maintenance of an ac ... | 1987 | 2886360 |
| [synthesis of new diheterocyclic derivatives of imidazole with antiparasitic activity]. | a description is given of potential cyclic imidazoles such as 3,3-dichloro-2-methylenimino-acrylonitrile derived from nitro or non-nitro heterocycles, as well as the hydrazone, semicarbazone and arylpropenone derivatives of the 4-chloro-2-(5-nitro-2-thienyl) (v), 5-imidazole carboxaldehyde (iv) and the 5-bromothienyl analogue. the antiparasitic activity was tested in vitro against trichomonas vaginalis and entameba histolytica. some of the nitro containing compounds showed trichomonacidal activi ... | 1987 | 2886364 |
| stimulation by target cell membrane lipid of actin polymerization and phagocytosis by entamoeba histolytica. | the identity of molecules of mammalian target cells that stimulate contact-dependent attack by entamoeba histolytica was sought using human erythrocytes (rbc) as a model. protein-free liposomes prepared from rbc membrane lipids stimulated the same rapid e. histolytica actin polymerization and phagocytosis as did whole target cells. liposomes constructed from the major phospholipids of rbc stimulated these responses but only if a negatively charged phospholipid was included. the addition to these ... | 1987 | 2886434 |
| enzyme linked protein-a: an elisa for detection of amoebic antibody. | enzyme linked protein-a was used to develop an enzyme linked immunosorbent assay (elisa) system for the detection of circulating antibodies to amoebic antigen. the specificity of protein-a to bind igg only through fc receptors, makes the test more specific for the detection of igg antibodies to amoebic antigen. the elisa system was used to detect amoebic antibody in control subjects (56), patients with amoebic liver abscess (79) and entamoeba histolytica cyst-passers (10) and the results compare ... | 1987 | 2887049 |
| physiochemical alteration of surface membrane of axenic entamoeba histolytica during repeated cholesterol passage. | 1987 | 2887508 | |
| elicitation of protective immunity to entamoeba histolytica--an experimental study. | immunization of hamsters with plasma membrane (pm) antigens of virulent subline of axenic entamoeba histolytica (nih: 200 v) entrapped in multilamellar phosphatidyl choline liposomes conferred 100% protection to a subsequent intrahepatic amoebic challenge. in contrast, vaccination of hamsters with live amoebic trophozoites injected intradermally failed to protect any of the animals. the protected animals had significantly high levels of anti-pm anti-amoebic antibodies, cellular sensitization and ... | 1987 | 2887509 |
| blastocystis hominis and human disease. | 1987 | 2887585 | |
| vaccination against experimental hepatic amoebic infection--an evaluation of phosphatidylcholine liposomes as immunopotentiator. | 1987 | 2887647 | |
| entamoeba histolytica: cloning and characterization of actin cdna. | in order to study gene expression in the human parasite entamoeba histolytica, a cdna library of e. histolytica strain 200:nih was constructed using the phage vector lambda gt10. three cdna clones (a, b and c) were selected for further analysis. each of the three clones hybridized to a distinct mrna. two of these mrnas were translated in vitro after hybrid selection, and yielded distinct translation products. one of these mrnas, selected by hybridization to clone a, encodes the most abundantly e ... | 1987 | 2888016 |
| [amebiasis: recent progress]. | 1987 | 2888181 | |
| a double-antibody sandwich elisa for the detection of entamoeba histolytica antigen in stool samples of humans. | a double-antibody sandwich elisa was developed to detect detergent-solubilized antigens of entamoeba histolytica in stool samples of humans. the test system was evaluated for its methodical and diagnostic sensitivity and specificity. in recovery experiments the lower limit of detection was 400 ng e. histolytica (hk9) protein/ml stool, corresponding to approximately 2000 amoebic trophozoites/ml stool. samples of 97 patients with suspected intestinal amoebiasis were examined. specific antigens wer ... | 1987 | 2888183 |
| [effect of the biotope and the intensity of invasion on the activity of various oxidation-reduction enzymes in entamoeba histolytica]. | 1987 | 2888240 | |
| [synthesis and antiparasitic activity of new nitro-5-imidazole derivatives]. | syntheses are given for derivatives of the type: 2-amino-1-[(1-methyl-5-nitro-2-imidazolyl) methyleneimino] imidazole, of new hydrazones of 1-methyl-5-nitroimidazole-2-carboxaldehyde and also for semicarbazones and a hydrazone of 1-methyl-5-nitro-2-(4-formyl-styryl)imidazole. | 1987 | 2888689 |
| recognition of the galactose- or n-acetylgalactosamine-binding lectin of entamoeba histolytica by human immune sera. | cure of amebic liver abscess is associated with resistance to recurrent invasive amebiasis and the development of a humoral and cell-mediated immune response. we determined whether human immune sera contain blocking antibody for the 170-kilodalton (kda) galactose or n-acetylgalactosamine (gal/galnac)-binding lectin of entamoeba histolytica. by western blot (immunoblot) of whole amebae subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, all eight immune sera studied here promi ... | 1987 | 2888730 |
| complement activation in patients with amebic liver abscess. | serum or plasma concentrations of components of the classical (c1q, c4) and alternative (c3, factor b) pathways, regulatory protein factor h, and one of the c3 products of degradation, c3d, were determined in 19 patients with amebic liver abscess (ala). patients were divided into two groups. thirteen patients that recovered under medical treatment who had a significantly shorter clinical course on admission (p less than 0.05) (group 1) exhibited either normal (c1q; c4; factor b; c3d) or increase ... | 1987 | 2888804 |
| isolation of a 220-kilodalton protein with lectin properties from a virulent strain of entamoeba histolytica. | a 220-kilodalton (kda) protein with lectin properties was isolated from entamoeba histolytica strain hm1:imss and was purified by sepharose 4b chromatography and electroelution from 5% sds-polyacrylamide gels. the protein contains 9% carbohydrate by weight; is rich in hydrophobic residues; and is very immunogenic in mice, hamsters, and rabbits. the protein binds to fixed monolayers of mdck cells and inhibits trophozoite attachment to the cultured cells. the 220-kda protein agglutinates human ery ... | 1987 | 2888825 |
| use of antibodies to characterize a 220-kilodalton surface protein from entamoeba histolytica. | antibodies were prepared against a 220-kilodalton (kda) protein partially purified by sepharose 4b chromatography of entamoeba histolytica strain hm38:imss homogenates, and the protein was found to have lectin properties. the antibodies specifically recognized this protein in trophozoite homogenates. immunologically related molecules with the same molecular weight were identified by polyclonal antibodies in strains hm1:imss, entamoeba invadens, and entamoeba histolytica laredo. six monoclonal an ... | 1987 | 2888826 |
| unloaded polyisobutylcyanoacrylate nanoparticles: efficiency against bloodstream trypanosomes. | the potential use of polyisobutylcyanoacrylate nanoparticles in antiparasitic chemotherapy is described. the nanoparticles on their own proved to have trypanocidal activity against trypanosoma brucei brucei in-vitro but not against trichomonas vaginalis or entamoeba histolytica. the trypanocidal activity was partly confirmed in-vivo with t. brucei-infected mice. | 1987 | 2888859 |
| a fast electrophoretic isoenzyme technique for the identification of invasive and non-invasive entamoeba histolytica and "e. histolytica-like" organisms. | cellulose acetate electrophoresis (cae) was used to separate glucosephosphate isomerase, hexokinase, malic enzyme, and phosphoglucomutase extracted from invasive and non-invasive entamoeba histolytica and "e. histolytica-like" organisms. each of these morphologically similar organisms possessed a unique cae isoenzyme profile that can be used as an aid in their identification. the cae technique used to obtain these isoenzyme profiles is rapid, simple, and economical, and it requires neither speci ... | 1987 | 2888886 |
| in vitro evaluation of the activity of entamoeba histolytica studies on motility. | the tube migration test for leukocyte function studies has been modified to test the motility of entamoeba histolytica. the test was more sensitive to the effect of antiamebic drugs than was a test for phagocytic activity. a 50% reduction in amebic motility was seen instantly after exposure to metronidazole (40 mg/l). a similar reduction of phagocytosis at the same concentration of the drug was seen only after two hours' preexposure. the extent of amebic migration in the test tube is dependent o ... | 1987 | 2889170 |
| immunological studies in amebiasis: isotypic characterization of specific antibodies by enzyme-linked immunofiltration assay. | fifty sera from 17 cases of invasive amebiasis (15 hepatic localizations, 1 ameboma, 1 diffused colic localization) were studied by enzyme-linked immunofiltration assay (elifa). igm and ige anti-entamoeba histolytica were detected in 10 and 15, respectively, of the 17 patients studied. igm and ige antibodies were found simultaneously in 10 cases; these 2 isotypes were only recognized twice in the same serum by the same antigenic components. during post-therapy monitoring, in less than 90 days ig ... | 1987 | 2889384 |
| the electrophoretic isoenzyme patterns of strains of entamoeba histolytica isolated in two major cities in canada. | the isoenzyme patterns of 92 isolates of entamoeba histolytica from british columbia and 28 from ontario were determined. seropositivity for e. histolytica was assessed by indirect hemagglutination and enzyme-linked immunosorbent assay in the one center and by elisa and amebic gel diffusion in the other. in both british columbia and ontario nonpathogenic zymodemes i and iii were most common. a newly described isoenzyme pattern was identified in ontario. only 9 of 120 zymodeme patterns identified ... | 1987 | 2889385 |
| cholesterol induced changes in glucose-6-phosphate generating enzymes, concanavalin a agglutinability and haemolytic activity of axenic entamoeba histolytica. | repeated passage of the 200-nih strain of entamoeba histolytica through cholesterol-enriched axenic growth medium induced marked increases in cholesterol, phosphoglucomutase and hexokinase levels and a less prominent rise in the protein content of amoebic cells. there was also pronounced enhancement of haemolytic activity and concanavalin a (con a) agglutinability of the culture, but no significant change was observed in glucose phosphate isomerase. these cholesterol-induced effects persisted to ... | 1987 | 2889429 |
| a simplified in vitro assay correlating amebicidal activity with chelation by 8-hydroxyquinoline and related compounds. | 1987 | 2889432 | |
| interaction of pathogenic and nonpathogenic entamoeba histolytica with human complement. | 1987 | 2889433 | |
| isoenzyme patterns of strains of entamoeba histolytica isolated in british columbia. | 1987 | 2889434 | |
| the reliability of entamoeba histolytica zymodemes in clinical laboratory diagnosis. | 1987 | 2889435 | |
| specificity of a cysteine proteinase of entamoeba histolytica towards the alpha 1-cb2 peptide of bovine collagen type i. | the cysteine proteinase of entamoeba histolytica was shown to hydrolyze the cyanogen bromide peptide alpha 1-cb2 of calf skin collagen type i yielding two split products. amino acid analyses of the formed peptides and estimation of the amino-terminal residues revealed that the alpha 1-cb2 peptide was exclusively cleaved between gly10 and leu11 within the sequence -arg9-gly10-leu11-yielding two peptides with 7 and 29 amino acids, respectively. under identical conditions the ratio of hydrolysis of ... | 1987 | 2890103 |
| isolation and partial characterization of the hexokinase isoenzymes from pathogenic and non-pathogenic strains of entamoeba histolytica. | isoenzyme electrophoretic patterns (zymodemes) are increasingly used to distinguish between pathogenic and non-pathogenic strains of entamoeba histolytica. isolates of e. histolytica from asymptomatic and symptomatic cases have been shown to differ in the electrophoretic mobility of their hexokinase and phosphoglucomutase isoenzymes. the hexokinase isoenzymes from a non-pathogenic strain and from a pathogenic strain of e. histolytica were purified by fast protein liquid chromatography in several ... | 1987 | 2890104 |
| [confirmation of the identity of intestinal amoebae and evaluation of the virulence of entamoeba histolytica by isoenzyme electrophoresis]. | 1987 | 2890195 | |
| alterations of humoral, cell mediated and antibody dependent cell mediated cytotoxic responses during the course of amoebic infection in guinea pigs. | the cellular and antibody dependent cellular cytotoxic (adcc) responses of splenic lymphocytes and peritoneal macrophages obtained from animals at variable intervals after inoculation were studied against trophozoites of axenic e histolytica (nih:200). cytotoxic responses of effector cells from infected animals were compared with those of effector cells from vaccine stimulated and unstimulated uninfected control animals. cellular and antibody-dependent cellular cytotoxic responses of the effecto ... | 1987 | 2890558 |
| isolation of the galactose-binding lectin that mediates the in vitro adherence of entamoeba histolytica. | entamoeba histolytica adheres to human colonic mucus, colonic epithelial cells, and other target cells via a galactose (gal) or n-acetyl-d-galactosamine (galnac) inhibitable surface lectin. blockade of this adherence lectin with gal or galnac in vitro prevents amebic killing of target cells. we have identified and purified the adherence lectin by two methods: affinity columns derivatized with galactose monomers or galactose terminal glycoproteins, and affinity columns and immunoblots prepared wi ... | 1987 | 2890654 |
| rat and human colonic mucins bind to and inhibit adherence lectin of entamoeba histolytica. | establishment of adherence by entamoeba histolytica is mediated by a 170-kd gal/galnac inhibitable lectin and is required for cytolysis and phagocytosis of mammalian target cells. we studied the biochemical mechanisms of the in vitro interaction between rat and human colonic mucins and axenic e. histolytica trophozoites. crude mucus prevented amebic adherence to chinese hamster ovary (cho) cells by up to 70%. purification of the colonic mucins by sepharose 4b chromatography, nuclease digestion, ... | 1987 | 2890655 |
| entamoeba histolytica cyst passers--to treat or not to treat? | 1987 | 2891196 | |
| a longitudinal study of asymptomatic carriers of pathogenic zymodemes of entamoeba histolytica. | the value of treating subjects who pass entamoeba histolytica cysts in their faeces is currently in question. in the endemic durban area iso-enzyme electrophoresis of e. histolytica isolates indicated that 1% of the asymptomatic population are infected with pathogenic zymodemes. the outcome of these potentially invasive infections was established by means of a longitudinal survey. all subjects had strongly positive serological responses--10% of them developed amoebic colitis while the rest remai ... | 1987 | 2891197 |
| cellular and humoral mechanisms of cytotoxicity: structural and functional analogies. | 1987 | 2891261 | |
| longitudinal study on the health status of children in a rural tanzanian community: parasitoses and nutrition following control measures against intestinal parasites. | three repeated cross-sectional surveys were undertaken among children (1 month to 15 years) of a rural community in southeastern tanzania. the study was part of a longitudinal project on the interactions among nutrition, parasitic infections and immunity within a primary health care programme emphasizing village health workers. all children underwent interviews and parasitological, anthropometric, anamnestic and clinical examinations. out of 550-590 children examined each year, a cohort of 170 c ... | 1987 | 2891267 |
| immunoprotective behaviour of plasma-membrane-associated antigens of axenic entamoeba histolytica. | immunisation of golden hamsters with plasma-membrane-associated antigens of a virulent subline of axenic entamoeba histolytica strain nih 200 v, entrapped in multilamellar phosphatidylcholine liposomes (mpl) or freund's complete adjuvant (fca), afforded protection against intrahepatic challenge with axenic amoebic trophozoites of the same strain. amoebic liver abscess developed in 86% and 80% of the animals that received empty liposomes or buffer emulsified in fca but in none of the animals that ... | 1987 | 2891855 |
| iga protease in entamoeba histolytica trophozoites. | 1987 | 2892356 | |
| antiamoebic properties of human colostrum. | 1987 | 2892357 | |
| [entamoeba histolytica surface antigens that interact with specific human iga immune sera]. | 1987 | 2892474 | |
| two-year survey of etiologic agents of diarrheal disease at san lazaro hospital, manila, republic of the philippines. | the prevalence of bacterial pathogens and rotavirus in 2,908 patients with diarrhea who were admitted to san lazaro hospital in manila in 1983 and 1984 was determined. one or more enteric pathogens were isolated or detected in samples from 1,698 (58.4%) patients. isolation rates for the various enteropathogens were as follows: rotavirus, 30.6%; shigella spp., 11.6%; salmonella spp., 9.2%; enterotoxigenic escherichia coli (1983 only), 7.8%; vibrio cholerae biotype eltor, 3.8%; non-o1 v. cholerae, ... | 1987 | 3038946 |
| increase in activity and biosynthesis of phospholipase a of entamoeba histolytica by cholesterol passage. | 1987 | 2894368 | |
| low susceptibility of trophozoites of virulent entamoeba histolytica to cellular and antibody-dependent cellular cytotoxicity by guinea-pig effector cells. | cytotoxic potentialities of lymphocytes and macrophages were determined against trophozoites of virulent and avirulent sublines of entamoeba histolytica in vitro. guinea-pigs were immunized with antigens of both sublines to obtain stimulated effector cells and antiamoebic antibodies. trophozoites of an avirulent/attenuated subline of e. histolytica (nih200) were significantly more susceptible to killing by lymphocytes and macrophages through cellular and antibody-dependent cellular cytotoxic mec ... | 1987 | 2902704 |
| [endoscopic diagnosis of a cecal ameboma successfully treated with antibiotic therapy]. | 1987 | 2902725 | |
| immunosuppression and splenomegaly in entamoeba histolytica infection in mice. | intestinal amoebiasis caused by entamoeba histolytica trophozoites in mice is accompanied by a depression in the ability of this host to develop an immune response to sheep red blood cells. the number of splenic plaque-forming cells was reduced in mice inoculated intracecally with 2.5 x 10(5) trophozoites at 15, 25, 40, 65 and 75 days after infection when compared with non-infected mice. it was found that there was no significant difference between the spleen weight of the infected and non-infec ... | 1987 | 2907086 |
| [interaction of entamoeba histolytica and antibodies in situ]. | 1987 | 2899424 | |
| [histochemical studies of lipid metabolism in entamoeba histolytica (schaudinn, 1903)]. | 1987 | 2900579 | |
| development of inflammation and augmented chemotactic responsiveness of murine peritoneal macrophages following treatment with entamoeba histolytica trophozoites. | the accumulation of inflammatory cells in the peritoneal cavity of c57bl/6 mice was examined following intraperitoneal injection of entamoeba histolytica trophozoites. two different strains of e. histolytica were used: a virulent strain (ip:0682:1) and a non-virulent strain (dkb). injection of 10(6) trophozoites of either strain resulted in significant increases in the numbers of total peritoneal cells, macrophages and polymorphonuclear cells as compared to either saline-injected control mice or ... | 1987 | 2904110 |
| amebic liver abscess and circulating immune complexes of entamoeba histolytica proteins. | an enzyme-linked immunosorbent assay (elisa) has been developed to detect amebic antigen in polyethylene-glycol-precipitable circulating immune complexes. seventy-nine percent of 191 patients with amebic liver abscesses and 46% of 26 entamoeba histolytica cyst passers had positive tests. none of the samples from 43 apparently healthy controls, 8 patients with past amebic liver abscesses or 31 patients with giardia lamblia infections were positive. one patient out of 32 with viral hepatitis and 1 ... | 1988 | 2904229 |
| a simple & rapid dot-elisa dipstick technique for detection of antibodies to entamoeba histolytica in amoebic liver abscess. | 1988 | 2904412 | |
| maintenance of integrity, viability, and adhesion of entamoeba histolytica trophozoites in different incubation media. | we have determined the integrity, viability and adhesion of entamoeba histolytica hk9 and hm1 trophozoites during their incubation in two basal culture media (tp and tyi) and three saline media ("maintenance medium" mm-1 and two others buffered with hepes). in basal culture media, more than 70% of the trophozoites maintained their integrity and adhesion to human red blood cells (rbc) for up to 4 h, and the proportion of those excluding trypan blue decreased slowly after 2 h. in saline media, the ... | 1988 | 2904497 |
| [synthesis and antiparasitic activity of arylproprenones in the nitro-5-imidazole series]. | the synthesis of new arylpropenones derived from 1-methyl-5-nitro imidazole is described. the investigation of some antiprotozoal properties has shown that compounds with the groups 4-hydroxyphenyl, 4-chlorophenyl or thiophenyl have trichomonacidal activity similar to that of metronidazole. | 1988 | 2904887 |
| clinical, microbiological and antibiotic susceptibility patterns of diarrhoea in korem, ethiopia. | two hundred patients with diarrhoea in a rehabilitation camp in ethiopia were studied in october 1985 to determine the presence of pathogens in the stool and their susceptibility to antibiotics. a total of 42 (21.1%) patients had a positive culture with enterobacteriaceae, the isolation rate was 15.6% for escherichia coli, 3.5% for shigella spp. and 2.01% for salmonella spp. in-vitro antibiotic resistance was frequent among the 42 isolates: 53% of e. coli strains were found to be resistant to am ... | 1988 | 2905012 |
| [mexican contributions to vaccines]. | 1988 | 2905313 | |
| [breast-feeding and intestinal parasites]. | the extent of intestinal parasitic infection was determined using 4 methods of fecal examination in 400 gabonese infants aged 0.5 to 24 months (86 exclusively and 151 partially breast-fed and 163 artificially fed) and in 170 of their mothers in an african urban community. parasitic prevalence was the same in the stools of the mothers from the 3 infant groups, providing evidence for the same level of exposure to parasites in all infants. exclusively and partially breast-fed infants excreted paras ... | 1988 | 2905884 |
| meleney's synergistic gangrene. | 1988 | 2905915 | |
| entamoeba histolytica infection in a rehabilitation center for mentally retarded persons in japan. | 1988 | 2906172 | |
| [intracellular location of vacuoles with acid phosphatase activity in trophozoites of entamoeba histolytica adhered to guinea pig cecal epithelium]. | 1988 | 2906533 | |
| [seroepidemiological study on amoebiasis]. | 1988 | 2906569 | |
| modulation of pathogenicity of entamoeba histolytica by histamine. | 1988 | 2906629 | |
| role of beta-glucuronidase, a lysosomal enzyme, in the pathogenesis of intestinal amoebiasis: an experimental study. | the role of beta-glucuronidase (bg), released by blood monocytes and kupffer cells, in the pathogenesis of intestinal amoebiasis was studied. guinea-pigs, infected intracaecally with entamoeba histolytica, were killed 0, 3, 7, 14 and 21 d after infection. enhanced levels of bg were observed in the cell lysates as well as supernatants of both blood monocytes and kupffer cells in the infected animals, from the 3rd day after infection, as compared to those in controls. the rise in bg levels was mor ... | 1988 | 2906765 |
| abdominal vein distension in amoebic liver abscess. | 1988 | 2906766 | |
| distribution of pathogenic zymodemes of entamoeba histolytica. | 1988 | 2906767 | |
| analysis of soluble entamoeba histolytica antigen using enzyme-l inked electroimmunotransfer blots. | soluble antigens of ten strains of e. histolytica were studied by sodium dodecylsulphate-polyacrylamide gel electrophoresis (sds-page) and enzyme-linked electroimmunotransfer blots (eitb). no relations of immune replicas to virulence, geographical origin and method of cultivation (xenic or axenic culture) were found. antigens of all ten strains tested precipitated with anti-e. histolytica human serum in the area of 30-43 kd. antigen of hk-9 strain created in this area a characteristic pattern wi ... | 1988 | 2906894 |
| [the morphology of amebiasis]. | amebiasis--a protozoan human disease caused by entamoeba histolytica--occurs in the ussr sporadically in particular geographic regions and in particular populations. the paper reports morphologic findings made in patients with amebiasis involving the intestine or other organs. in typical cases, amebiasis affects the large intestine, particularly the cecum and ascending colon, and has the development of distinctive ulcers as its characteristic feature. as the disease progresses, these appear in a ... | 1988 | 2900628 |
| entamoeba histolytica antigen-specific induction of human immunodeficiency virus replication. | replication of human immunodeficiency virus (hiv) may be initiated in infected lymphocytes by antigenic or mitogenic stimulation. a soluble protein derived from an invasive strain of entamoeba histolytica (amoebic antigen [aa]) was used to study the lymphoblastic responses of t lymphocytes derived from 8 hiv-seronegative homosexual men (controls) and 15 hiv-seropositive homosexual men (patients). the soluble protein was also used in long-term cultures as a stimulus for hiv replication. no contro ... | 1988 | 2900844 |
| trophozoite and nuclear size, dna base composition, and nucleotide sequence homology of several entamoeba strains in axenic culture. | we carried out a comparative study of nuclear and trophozoite diameters and of dna thermal denaturation in eight entamoeba strains cultured axenically (four of them e. histolytica, two initially designated as e. invadens, one e. moshkovskii, and one e. histolytica-like), as well as an analysis of the overall dna sequence homology of the non-e. histolytica strains. the average nuclear (n) and trophozoite (t) diameters (in micron) were, respectively: global averages +/- sd for e. histolytica strai ... | 1988 | 2901079 |
| [a modified tanabe-chiba medium for detection of entamoeba histolytica]. | tanabe-chiba's (t-c) medium, which has been routinely used for detection of entamoeba histolytica in feces in japan, was modified by adding some commercially available enrichments to the liquid part and by eliminating the agar slant. this is tentatively called m medium. m medium and t-c medium were compared for their efficiency for detecting e. histolytica in the feces of monkeys. no significant difference in the detection rate was found between the two media. however the rate of multiplication ... | 1988 | 2901365 |
| evaluation of enzyme-linked immunosorbent assay (elisa), indirect hemagglutination (iha), counterimmunoelectrophoresis (ciep), and immunodiffusion (id) in the serodiagnosis of amebiasis. | 1988 | 2901446 | |
| isolated amebic appendicitis: a pathologic rarity. | 1988 | 2901812 | |
| entamoeba histolytica: cell cycle and nuclear division. | the cell cycle of entamoeba histolytica, the duration of its phases, and the details of the nuclear division stages are described in this paper. trophozoites from clone l-6, strain hm1:imss, were synchronized by colchicine. synchrony was observed immediately after treatment and cultures remained synchronous for at least three replicative cycles with synchrony indexes between 13 and 15 hr. the stages of nuclear division were studied by light and electron microscopy. four stages of the nuclear div ... | 1988 | 2901981 |
| entamoeba histolytica: virulence potential and sensitivity to metronidazole and emetine of four isolates possessing nonpathogenic zymodemes. | the pathogenic potential of four entamoeba histolytica isolates obtained from asymptomatic carriers and possessing nonpathogenic zymodemes was compared to four e. histolytica strains obtained from invasive cases of amebiasis and having pathogenic zymodemes. both xenic and axenic cultures of a number of strains were tested. determinations of cytopathogenicity were done in vitro by measuring the rates of destruction of tissue cultured monolayers of baby hamster kidney cells by intact amebae or by ... | 1988 | 2899517 |
| entamoeba histolytica: potent in vitro antiamoebic effect of gossypol. | 1988 | 2899518 | |
| freeze-fracture study of the trophozoite and the cyst of entamoeba histolytica. | the fine structure of the trophozoite and cyst of entamoeba histolytica from the stool of a patient was compared using the freeze-fracture method. the intramembranous particles (imp's) were heterogeneously distributed on the plasma membrane of the trophozoite and their density was 1139 +/- 105/micron 2 on the p face. particle-rich depressions and linear particle arrays, reported by other investigators on cultured trophozoites, were also observed on the p face while on the e face such special par ... | 1988 | 2899642 |
| susceptibility of hamsters to caecal amoebiasis by oral infection. | a method is described for successfully establishing caecal amoebiasis in hamsters which were not fed and which were pretreated with 1 ml of magnesium sulphate every 24 hours for three days and then given 12 x 10(5) trophozoites of the hm-1 axenic strain or 18 x 10(5) trophozoites of the hk-9 axenic strain of entamoeba histolytica by the oral route. all the animals developed diarrhoea within 24 hours of infection. when the animals were killed on the fifth day after infection the caecum was swolle ... | 1988 | 2899994 |
| entamoeba histolytica antigens as possible vaccinogens? a short review. | a few key papers which have recently been published on the characterization of amoeba antigens are reviewed. immunofluorescence tests and immunoelectron microscopy have demonstrated the localization of certain surface antigens on axenically cultured trophozoites. most of the surface antigens have largely been shown to elicit a humoral response. the elicitation of cellular response has not been well illustrated. the localization of a large number of antigens in cytoplasmic vacuoles and plasma mem ... | 1988 | 2900249 |