Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| safety evaluation of a heavy oil-degrading bacterium, rhodococcus erythropolis c2. | the safety of an oil-degrading bacterium, c2 strain, was evaluated for utilization in an open system for bioremediation of oil-contaminated environments. the c2 strain was identified as rhodococcus erythropolis by performing an alignment analysis of the whole 16s rrna sequence. r. erythropolis was classified as a nonpathogenic (category 1) bacterium. biological and biochemical properties of the c2 strain also confirmed its nonpathogenicity. the pathogenicity and basic ecotoxicity were studied in ... | 2007 | 17327695 |
| structural and mechanistic analyses of endo-glycoceramidase ii, a membrane-associated family 5 glycosidase in the apo and gm3 ganglioside-bound forms. | endo-glycoceramidase, a membrane-associated family 5 glycosidase, deviates from the typical polysaccharide substrate specificity of other soluble members of the family, preferentially hydrolyzing glycosidic linkages between the oligosaccharide and ceramide moieties of gangliosides. here we report the first x-ray crystal structures of an endo-glycoceramidase from rhodococcus sp., in the apo form, in complex with the ganglioside g(m3) (svennerholm ganglioside nomenclature (svennerholm, l. (1964) j ... | 2007 | 17329247 |
| purification and characterization of the enantioselective nitrile hydratase from rhodococcus sp. aj270. | nitrile hydratase (nhase, ec 4.2.1.84) from rhodococcus sp. aj270 was purified with 23.96% yield after sonication, ammonium sulfate fractionation, ion exchange, hydrophobic and gel-filtration column chromatography. the enzyme showed intriguing characteristics: it hydrated not only aliphatic and heterocyclic nitriles but also aromatic ones. some substrates were also hydrated enantioselectively to the corresponding amides. the enantiomeric excess (ee) value of the enzyme hydrating trans-2,2-dimeth ... | 2007 | 17330219 |
| comparative studies of phenotypic and genetic characteristics between two desulfurizing isolates of rhodococcus erythropolis and the well-characterized r. erythropolis strain igts8. | two rhodococcus erythropolis isolates, named a66 and a69, together with the well-characterized r. erythropolis strain igts8 were compared biochemically and genetically. both isolates, like strain igts8, desulfurized dbt to 2-hydroxybiphenyl (2-hbp), following the 4s pathway of desulfurization. strain igts8 showed the highest (81.5%) desulfurization activity in a medium containing dbt at 30 degrees c. strain a66 showed approximately the same desulfurization activity either when incubated at 30 de ... | 2007 | 17333091 |
| diversity of cultivated and uncultivated actinobacterial endophytes in the stems and roots of rice. | a dual approach consisting of cultivation and molecular retrieval of actinobacterial 16s rrna genes was used to characterize the diversity of actinobacterial community inhabiting interior of rice stems and roots. streptomyces is the most frequently isolated genus from rice stems and roots. forty-five clones chosen randomly among 250 clones in the 16s rrna gene clone library from roots were affiliated with nine genera of actinobacteria and uncultured actinobacteria (mycobacterium, streptomyces, m ... | 2007 | 17334856 |
| bacteria transport and deposition under unsaturated conditions: the role of the matrix grain size and the bacteria surface protein. | unsaturated (80% water saturated) packed column experiments were conducted to investigate the influence of grain size distribution and bacteria surface macromolecules on bacteria (rhodococcus rhodochrous) transport and deposition mechanisms. three sizes of silica sands were used in these transport experiments, and their median grain sizes were 607, 567, and 330 microm. the amount of retained bacteria increased with decreasing sand size, and most of the deposited bacteria were found adjacent to t ... | 2007 | 17337313 |
| characterization of the dszabc genes of gordonia amicalis f.5.25.8 and identification of conserved protein and dna sequences. | gordonia amicalis f.5.25.8 has the unique ability to desulfurize dibenzothiophene and to metabolize carbazole [santos et al., appl microbiol biotechnol 71:355-362, 2006]. efforts to amplify the dsz genes from g. amicalis f.5.25.8 based on polymerase chain reaction (pcr) primers designed using the dsz gene sequences of rhodococcus erythropolis igts8 were mostly unsuccessful. a comparison of the protein sequences of dissimilar desulfurization enzymes (dszabc, bdsabc, and tdsabc) revealed multiple ... | 2007 | 17342529 |
| steroid 9alpha-hydroxylation during testosterone degradation by resting rhodococcus equi cells. | the conversion pathway of testosterone to androst-4-ene-3,17-dione and 9alpha-hydroxy androstane metabolites, 9alpha-hydroxyandrost-4-ene-3,17-dione and 9alpha,17beta-dihydroxyandrost-4-en-3-one was proposed for the ring degradation in steroids by a minimal liquid medium (nmmp)-dispersed rhodococcus equi atcc 14887. the microorganism produced 9alpha-hydroxy androstane metabolites from testosterone at high conversion ratio without the addition of ring degradation inhibitory agents. several nmmp-b ... | 2007 | 17351967 |
| genotypic characterization of vapa positive rhodococcus equi in foals with pulmonary affection and their soil environment on a warmblood horse breeding farm in germany. | pulsotypes of vapa positive rhodococcus equi isolated from foals and soil on a farm in germany were characterized on the basis of nasal and tracheal samples simultaneously collected in 2003 from 217 foals with sonographic evidence of pneumonia or pulmonary abscesses. of the 217 double samples, r. equi was isolated in 118 (54%) of the tracheal samples and in 52 of the nasal swab samples (24%) (p<0.001). furthermore, 37 and 55 isolates were also randomly selected from nasal swabs and the tracheal ... | 2007 | 17360011 |
| improved outcome of trypanosoma cruzi infection in rats following treatment in early life with suspensions of heat-killed environmental actinomycetales. | the well-established model of chagas' disease in "l" rats was used to evaluate the effects of three injections of heat-killed gordonia bronchialis, rhodococcus coprophilus or saline on trypanosoma cruzi parasitaemia and acute and chronic myocarditis, sequelae of the infection. two vaccinating injections were given prior to challenge with t. cruzi, and the third, immunotherapeutic, injection was given 7 days after challenge. treatment with either actinomycete significantly reduced acute parasitae ... | 2007 | 17368877 |
| bioaugmentation and coexistence of two functionally similar bacterial strains in aerobic granules. | the survival of the inoculated microbial culture is critical for successful bioaugmentation but impossible to predict precisely. as an alternative strategy, bioaugmentation of a group of microorganisms may improve reliability of bioaugmentation. this study evaluated simultaneous bioaugmentation of two functionally similar bacterial strains in aerobic granules. the two strains, pandoraea sp. pg-01 and rhodococcus erythropolis pg-03, showed high phenol degradation and growth rates in phenol medium ... | 2007 | 17370069 |
| functional identification of p-cumate operons in the terpene-degrading rhodococcus sp. strain t104. | we identified a p-cumate degrading gene cluster (designated cmt) with a novel organization in our genomic studies of the terpene-degrading rhodococcus sp. strain t104. the mutant strain sn140, isolated for the inability to grow on p-cumate, accumulates 2,3-dihydroxy-p-cumate as identified by liquid chromatography-mass spectrometry and 300 mhz proton nuclear magnetic resonance spectroscopy. reverse transcriptase polymerase chain reaction experiments showed that the cmt genes are operonic and indu ... | 2007 | 17233717 |
| the structure of a bacterial l-amino acid oxidase from rhodococcus opacus gives new evidence for the hydride mechanism for dehydrogenation. | l-amino acid oxidase from rhodococcus opacus (rolaao) is classified as a member of the gr(2)-family of flavin-dependent oxidoreductases according to a highly conserved sequence motif for the cofactor binding. the monomer of the homodimeric enzyme consists of three well-defined domains: the fad-binding domain corresponding to a general topology throughout the whole gr(2)-family; a substrate-binding domain with almost the same topology as the snake venom laao and a helical domain exclusively respo ... | 2007 | 17234209 |
| a novel endoglycoceramidase hydrolyzes oligogalactosylceramides to produce galactooligosaccharides and ceramides. | enzymes capable of hydrolyzing the beta-glycosidic linkage between oligosaccharides and ceramides in various glycosphingolipids has been found in microorganisms and invertebrates and designated endoglycoceramidase (ec 3.2.1.123) or ceramide glycanase. here we report the molecular cloning, characterization, and homology modeling of a novel endoglycoceramidase that hydrolyzes oligogalactosylceramides to produce galactooligosaccharides and ceramides. the novel enzyme was purified from a culture sup ... | 2007 | 17244618 |
| biodegradation of methylthio-s-triazines by rhodococcus sp. strain fj1117yt, and production of the corresponding methylsulfinyl, methylsulfonyl and hydroxy analogues. | a novel bacterial strain fj1117yt was isolated from an enrichment culture with the herbicide simetryn. the isolate was capable of degrading the herbicide supplied as the sole sulfur source in an aquatic batch culture. the strain fj1117yt was identified as that belonging to rhodococcus sp. on the basis of comparative morphology, physiological characteristics and comparison of the 16s rrna gene sequence. the biodegradation pathway of simetryn was established by isolating the methylsulfinyl analogu ... | 2007 | 17245693 |
| bacterial preparation of enantiopure unactivated aziridine-2-carboxamides and their transformation into enantiopure nonnatural amino acids and vic-diamines. | [reaction: see text] enantiopure (1r,2s)-1-benzyl- and 1-arylaziridine-2-carboxamides were obtained by kinetic resolution of their racemates by rhodococcus rhodochrous ifo 15564 catalyzed hydrolysis. several regio- and enantioselective nucleophilic ring openings of (1r,2s)-1-benzylaziridine-2-carboxamide or its lah-reduced product led to a series of enantiopure products, such as o-methyl-l-serine and some vicinal diamines. | 2007 | 17249802 |
| a gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into mycobacterium tuberculosis survival in macrophages. | rhodococcus sp. strain rha1, a soil bacterium related to mycobacterium tuberculosis, degrades an exceptionally broad range of organic compounds. transcriptomic analysis of cholesterol-grown rha1 revealed a catabolic pathway predicted to proceed via 4-androstene-3,17-dione and 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (3,4-dhsa). inactivation of each of the hsac, supab, and mce4 genes in rha1 substantiated their roles in cholesterol catabolism. moreover, the hsac(-) mutant accum ... | 2007 | 17264217 |
| daptomycin susceptibility of unusual gram-positive bacteria: comparison of results obtained by the etest and the broth microdilution method. | mics of daptomycin, linezolid, and vancomycin against 212 isolates, including listeria monocytogenes and pediococcus, leuconostoc, rhodococcus, and nocardia spp., were determined by the broth microdilution method; daptomycin mics were also determined by the etest. except with those for leuconostoc spp., daptomycin etest mics showed >90% agreement with mics obtained by the broth microdilution method. | 2007 | 17220420 |
| bioconversion of butyronitrile to butyramide using whole cells of rhodococcus rhodochrous pa-34. | butyramide is an important chemical commodity, which is used for the synthesis of hydroxamic acids and electrorheological fluids and for the preparation of beta-amodoorganotin compounds. the nitrile hydratase (nhase) of rhodococcus rhodochrous pa-34 catalyzed the conversion of butyronitrile to butyramide. the maximum nhase activity [18 u/mg dry cell weight (dcw)] of whole cells of r. rhodochrous pa-34 was observed at ph 7.0 with 10% (v/v) butyronitrile and 1 mg cells (dcw)/ml reaction mixture at ... | 2007 | 17216468 |
| transcriptomic analysis reveals a bifurcated terephthalate degradation pathway in rhodococcus sp. strain rha1. | phthalate isomers and their esters are important pollutants whose biodegradation is not well understood. rhodococcus sp. strain rha1 is notable for its ability to degrade a wide range of aromatic compounds. rha1 was previously shown to degrade phthalate (pth) and to have genes putatively encoding terephthalate (tpa) degradation. transcriptomic analysis of 8,213 genes indicated that 150 were up-regulated during growth on pth and that 521 were up-regulated during growth on tpa. distinct ring cleav ... | 2007 | 17142403 |
| enzymatic and genetic profiles in environmental strains grown on polycyclic aromatic hydrocarbons. | the possible generation of oxidative stress induced by aromatic hydrocarbon degradation suggests that ancillary enzyme activities could facilitate the utilization of polycyclic aromatic hydrocarbons as sole carbon source. to investigate the metabolic profiles of low molecular weight polycyclic aromatic hydrocarbon-degrading strains of sphingobium chlorophenolicum, rhodococcus aetherovorans, rhodococcus opacus and mycobacterium smegmatis, the determination of the activity of putative detoxifying ... | 2007 | 17109059 |
| benzoate catabolite repression of the phthalate degradation pathway in rhodococcus sp. strain dk17. | rhodococcus sp. strain dk17 exhibits a catabolite repression-like response when provided simultaneously with benzoate and phthalate as carbon and energy sources. benzoate in the medium is depleted to detection limits before the utilization of phthalate begins. the transcription of the genes encoding benzoate and phthalate dioxygenase paralleled the substrate utilization profile. two mutant strains with defective benzoate dioxygenases were unable to utilize phthalate in the presence of benzoate, ... | 2007 | 17158614 |
| malakoplakia of the prostate on needle core biopsy: a case report and review of the literature. | malakoplakia is a rare inflammatory disorder that results from an infectious process and that may be associated with immunosuppression. it most often occurs in the genitourinary tract; however, isolated cases have been reported in many other organs, including colon, stomach, lung, liver, bone, uterus, and skin. in the bladder, which is the most common site of involvement, it may be mistaken for malignancy on cystoscopic examination or computerized topography scan. gram-negative bacteria such as ... | 2007 | 17172507 |
| multiple-stress tolerance of ionizing radiation-resistant bacterial isolates obtained from various habitats: correlation between stresses. | isolation of five ionizing radiation (ir)-resistant bacteria by screening of isolates from various habitats classified as common and stressed is reported. ir-resistant isolates exhibited varying degrees of resistance to gamma-radiation and were classified as highly and moderately radiation resistant. resistance to ultraviolet (uv) radiation correlated well with gamma-radiation resistance, whereas a comparable desiccation resistance for all the highly and moderately radiation-resistant isolates w ... | 2007 | 17180747 |
| enhancing cell survival of atrazine degrading rhodococcus erythropolis ni86/21 cells encapsulated in alginate beads. | to develop a method to produce beads with encapsulated rhodococcus erythropolis ni86/21 with high cell density, extended shelf life, ease of handling and good atrazine degradation capabilities in both liquid and in agricultural soil. | 2007 | 17184337 |
| mutation and virulence assessment of chromosomal genes of rhodococcus equi 103. | rhodococcus equi can cause severe or fatal pneumonia in foals as well as in immunocompromised animals and humans. its ability to persist in macrophages is fundamental to how it causes disease, but the basis of this is poorly understood. to examine further the general application of a recently developed system of targeted gene mutation and to assess the importance of different genes in resistance to innate immune defenses, we disrupted the genes encoding high-temperature requirement a (htra), nit ... | 2007 | 17193875 |
| structural characterization of a beta-diketone hydrolase from the cyanobacterium anabaena sp. pcc 7120 in native and product-bound forms, a coenzyme a-independent member of the crotonase suprafamily. | the gene alr4455 from the well-studied cyanobacterium anabaena sp. pcc 7120 encodes a crotonase orthologue that displays beta-diketone hydrolase activity. anabaena beta-diketone hydrolase (abdh), in common with 6-oxocamphor hydrolase (och) from rhodococcus sp. ncimb 9784, catalyzes the desymmetrization of bicyclo[2.2.2]octane-2,6-dione to yield [(s)-3-oxocyclohexyl]acetic acid, a reaction unusual among the crotonase superfamily as the substrate is not an acyl-coa thioester. the structure of abdh ... | 2007 | 17198383 |
| evaluation of fecal samples from mares as a source of rhodococcus equi for their foals by use of quantitative bacteriologic culture and colony immunoblot analyses. | to determine whether mares are a clinically important source of rhodococcus equi for their foals. | 2007 | 17199420 |
| degradation of 2,5-dimethylpyrazine by rhodococcus erythropolis strain dp-45 isolated from a waste gas treatment plant of a fishmeal processing company. | a bacterium, strain dp-45, capable of degrading 2,5-dimethylpyrazine (2,5-dmp) was isolated and identified as rhodococcus erythropolis. the strain also grew on many other pyrazines found in the waste gases of food industries, like 2,3-dimethylpyrazine (2,3-dmp), 2,6-dimethylpyrazine (2,6-dmp), 2-ethyl-5(6)-dimethylpyrazine (emp), 2-ethylpyrazine (ep), 2-methylpyrazine (mp), and 2,3,5-trimethylpyrazine (tmp). the strain utilized 2,5-dmp as sole source of carbon and nitrogen and grew optimally at ... | 2007 | 17120096 |
| utilization of hydrophobic bacterium rhodococcus opacus b-4 as whole-cell catalyst in anhydrous organic solvents. | rhodococcus opacus strain b-4, which has recently been isolated as an organic solvent-tolerant bacterium, has a high hydrophobicity and exhibits a high affinity for hydrocarbons. this bacterium was able to survive for at least 5 days in organic solvents, including n-tetradecane, oleyl alcohol, and bis(2-ethylhexyl) phthalate (behp), which contained water less than 1% (w/v). the biocatalytic ability of r. opacus b-4 was demonstrated in the essentially nonaqueous behp using indigo production from ... | 2007 | 17123076 |
| granulomatous lesions in lymph nodes of slaughter pigs bacteriologically negative for mycobacterium avium subsp. avium and positive for rhodococcus equi. | the prevalence of granulomatous lesions in lymph nodes of pigs was studied. from january till august 2004 in two slaughterhouses in the netherlands 2,116,536 pigs were examined for the presence of granulomatous lesions in the sub-maxillary lymph nodes. in 15,900 (0.75%) of these pigs, lesions could be detected. nine farms with the highest incidence of lesions were selected for a more detailed pathological and bacteriological examination. on these farms, the prevalence of lesions in sub-maxillary ... | 2007 | 17126501 |
| remaining acetamide in acetonitrile degradation using nitrile hydratase- and amidase-producing microorganisms. | the tandem conversion process involving nitrile hydratase- and amidase-producing microorganisms has potential for use in the treatment of acetonitrile-containing wastes. in that process, the acetamide hydrolysis step catalyzed by amidase is very slow compared with the acetonitrile hydration step catalyzed by nitrile hydratase, and a small amount of acetamide remains in the resulting solution. this study aimed to improve the efficiency of the acetamide hydrolysis step. an amidase-producing microo ... | 2007 | 17136368 |
| sulfur from benzothiophene and alkylbenzothiophenes supports growth of rhodococcus sp. strain jvh1. | rhodococcus sp. strain jvh1 was previously reported to use a number of compounds with aliphatic sulfide bridges as sulfur sources for growth. we have shown that although jvh1 does not use the three-ring thiophenic sulfur compound dibenzothiophene, this strain can use the two-ring compound benzothiophene as its sole sulfur source, resulting in growth of the culture and loss of benzothiophene. addition of inorganic sulfate to the medium reduced the conversion of benzothiophene, indicating that ben ... | 2007 | 17091342 |
| selectivity among organic sulfur compounds in one- and two-liquid-phase cultures of rhodococcus sp. strain jvh1. | the selectivity of rhodococcus sp. strain jvh1 among selected sulfidic and thiophenic compounds was investigated in both single-liquid-phase (aqueous) cultures and in two-liquid-phase cultures, where the sulfur compounds were dissolved in 2,2,4,4,6,8,8-heptamethylnonane as the immiscible organic carrier phase. in the single-liquid-phase cultures, rhodococcus sp. strain jvh1 showed a preference for benzyl sulfide over both 1,4-dithiane and benzothiophene. an increased lag was observed in the degr ... | 2007 | 17091345 |
| biodegradation kinetics of the nitramine explosive cl-20 in soil and microbial cultures. | the cyclic nitramine explosive cl-20 (c(6)h(6)n(12)o(12), 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12 -hexaazaisowurtzitane) is a relatively new energetic compound which could be a persistent organic pollutant. to follow its biodegradation dynamics, cl-20 was added to soil alone or together with organic co-substrates and n-source and incubated under oxic and anoxic conditions. without co-substrates, the cl-20 degradation was detectable only under anoxic conditions. the highest degradation rate was fou ... | 2007 | 17091356 |
| extensive desulfurization of diesel by rhodococcus erythropolis. | the resting cells of a new isolate of rhodococcus erythropolis fsd-2 were used to desulfurize diesel fuels. about 97% of the total sulfur content in the hydrodesulfurized diesel was removed by the two consecutive biodesulfurization (bds) processes with the majority ( approximately 94%) being removed in the first treatment, resulting in diesel with a sulfur content of 5.7 microg ml(-1). | 2007 | 17091384 |
| effect of introduced phthalate-degrading bacteria on the diversity of indigenous bacterial communities during di-(2-ethylhexyl) phthalate (dehp) degradation in a soil microcosm. | four previously isolated di-butyl-phthalate (dbp) degraders were tested for their abilities to degrade di-(2-ethylhexyl) phthalate (dehp). in aqueous medium supplemented with 100mg/l of dehp, both isolate g1 and rhodococcus rhodochrous g2 showed excellent degradative activity; in three days they were able to degrade more than 97% of the added dehp. rhodococcus rhodochrous g7 degraded 32.5% of the added dehp and corynebacterium nitrilophilus g11 showed the least amount of dehp degradation. the ad ... | 2007 | 17092544 |
| a multipurpose transposon-based vector system mediates protein expression in rhodococcus erythropolis. | in the current study we developed two transposon-based vectors; namely ptnr-ka and ptnr-ta and utilized them for expression of proteasome complex, derived from streptomyces coelicolor, in rhodococcus erythropolis. the two vectors can be transposed into rhodococcus cells by means of electroporation, either individually in two consecutive processes or in combinations by a single step. during transposition, each of the two vectors liberates its transposable-marker gene, which integrated in a single ... | 2007 | 17098379 |
| comparison of two selective media for the recovery, isolation, enumeration and differentiation of rhodococcus equi. | the use of selective media to facilitate the isolation of rhodococcus equi from environmental and clinical samples has aided studies of the ecology of r. equi and the epidemiology of disease caused by r. equi. here, we compared the efficacy of two selective media (nanat and modified caz-nb) for the recovery of six defined strains of r. equi and for the isolation and enumeration of both avirulent and virulent r. equi from 60 paired soil samples from horse farms using colony blotting and dna hybri ... | 2007 | 17084043 |
| in vitro activities of the lipopeptides palmitoyl (pal)-lys-lys-nh(2) and pal-lys-lys alone and in combination with antimicrobial agents against multiresistant gram-positive cocci. | the in vitro activities of the lipopeptides palmitoyl (pal)-lys-lys-nh(2) and pal-lys-lys against gram-positive cocci were investigated. enterococci and streptococci demonstrated higher susceptibilities than staphylococci and rhodococcus equi. a positive interaction was shown when the lipopeptides were combined with beta-lactams and vancomycin. these results suggest that lipopeptides are promising candidates for antimicrobial therapy for infections caused by gram-positive organisms. | 2007 | 17060532 |
| a screening system for active and enantioselective amidase based on its acyl transfer activity. | a novel enantioselective amidase screening system was developed and proved to be efficient and accurate. this screening system employed acyl transfer activity of amidase in the presence of hydroxylamine, leading to the formation of hydroxamic acids, followed by spectrophotometric quantification of hydroxamic acid/iron(iii) complexes. the enantioselectivities of amidase were evaluated by employing (r, s)-2, 2-dimethyl cyclopropanecarboxamide (1), (s)-2, 2-dimethyl cyclopropanecarboxamide and thei ... | 2007 | 17033773 |
| analysis of the 7.6-kb cryptic plasmid pnc500 from rhodococcus rhodochrous b-276 and construction of rhodococcus-e. coli shuttle vector. | four circular cryptic plasmids were detected from propene-degrading rhodococcus rhodochrous (formerly nocardia corallina) b-276 and the smallest 7.6-kb plasmid, named pnc500 was used to construct rhodococcus-e. coli shuttle vector, pnc5403. sequence analysis of pnc500 revealed that the plasmid contains eight potential orfs, namely 1 through 8. the deduced amino acid sequences for orfs 3, 4, 6, and 7 show homology with those of rep a, rep b, dna methyl-transferase (m.xami), and restriction nuclea ... | 2007 | 17043815 |
| cloning, sequencing, and expression of a novel epoxide hydrolase gene from rhodococcus opacus in escherichia coli and characterization of enzyme. | an epoxide hydrolase gene of about 0.8 kb was cloned from rhodococcus opacus ml-0004, and the open reading frame (orf) sequence predicted a protein of 253 amino acids with a molecular mass of about 28 kda. an expression plasmid carrying the gene under the control of the tac promotor was introduced into escherichia coli, and the epoxide hydrolase gene was successfully expressed in the recombinant strains. some characteristics of purified recombinant epoxide hydrolase were also studied. epoxide hy ... | 2007 | 17043819 |
| a taqman polymerase chain reaction method for monitoring rdx-degrading bacteria based on the xpla functional gene. | hexahydro-1,3,5-trinitro-1,3,5,-triazine (rdx) is a cyclic nitramine explosive that is a major component in many military high-explosive formulations. in this study, we developed a real-time taqman polymerase chain reaction (pcr) that targets the xpla functional gene involved in the breakdown/transformation of rdx. the xpla gene, described previously [seth-smith, h.m., rosser, s.j., basran, a., travis, e.r., dabbs, e.r., nicklin s., bruce, n.c., 2002. cloning, sequencing, and characterization of ... | 2007 | 17010461 |
| rhodococcus equi infection during treatment of a c-anca positive vasculitis: a case report. | rhodococcus equi is a rare form of opportunistic infection in humans, more common in the immunocompromised. we present a case of pulmonary infection and subsequent cerebral abscess secondary to r. equi in a patient receiving immunosuppression for a c-anca positive vasculitis. heightened awareness of r. equi infection is important when considering immunocompromised patients presenting with sepsis, especially those with cavitating pulmonary lesions and normal respiratory commensals on culture. del ... | 2007 | 17013608 |
| post-translational cleavage of recombinantly expressed nitrilase from rhodococcus rhodochrous j1 yields a stable, active helical form. | nitrilases convert nitriles to the corresponding carboxylic acids and ammonia. the nitrilase from rhodococcus rhodochrous j1 is known to be inactive as a dimer but to become active on oligomerization. the recombinant enzyme undergoes post-translational cleavage at approximately residue 327, resulting in the formation of active, helical homo-oligomers. determining the 3d structure of these helices using electron microscopy, followed by fitting the stain envelope with a model based on homology wit ... | 2007 | 17371547 |
| [rhodococcus equi infection in subjects infected with human immunodeficiencv virus (hiv)]. | disease caused by rhodococcus equi is a rare complication in subjects infected with human immunodeficiency virus (hiv) and it is associated with severe cellular immunodeficiency. the agent is gram-positive rod of the group non-diphtheric corynebacteria. the genus rhodococcus belongs to the family nocardiaceae and order actinomycetales. the principle of pathogenicity is the survival inside macrophages. formation of necrotising granulomas is a characteristic feature. malacoplakia can be a specific ... | 2007 | 17373114 |
| adult onset of t-cell deficiency with impaired cd2 expression complicated by rhodococcus infection: a case report. | the functional importance of cd2 in vivo is currently the subject of discussion. | 2007 | 17378264 |
| culturable bacteria present in the fluid of the hooded-pitcher plant sarracenia minor based on 16s rdna gene sequence data. | the culturable microbial community within the pitcher fluid of 93 sarracenia minor carnivorous plants was examined over a 2-year study. many aspects of the plant/bacterial/insect interaction within the pitcher fluid are minimally understood because the bacterial taxa present in these pitchers have not been identified. thirteen isolates were characterized by 16s rdna sequencing and subsequent phylogenetic analysis. the proteobacteria were the most abundant taxa and included representatives from s ... | 2007 | 17380356 |
| separation of bacteria in temperature gradient: micro-thermal focusing field-flow fractionation. | the separation of staphylococcus epidermidis and rhodococcus erythropolis bacteria was achieved with the use of micro-thermal focusing field-flow fractionation. this is the first performance of separation exploiting the ludwig-soret effect (thermal diffusion) of living biological cells, combined with lift forces and resulting in the focusing mechanism of separation. the experiments were carried out under carefully chosen experimental conditions preventing the denaturation of the bacteria. | 2007 | 17391770 |
| experimental infection of neonatal foals with rhodococcus equi triggers adult-like gamma interferon induction. | rhodococcus equi is a facultative intracellular pathogen that causes pneumonia in young foals but does not induce disease in immunocompetent adult horses. clearance of r. equi depends mainly on gamma interferon (ifn-gamma) production by t lymphocytes, whereas the predominance of interleukin 4 (il-4) is detrimental. young foals, like neonates of many other species, are generally deficient in the ability to produce ifn-gamma. the objective of this study was to compare the cytokine profiles, as wel ... | 2007 | 17409222 |
| variations in 13c/12c and d/h enrichment factors of aerobic bacterial fuel oxygenate degradation. | reliable compound-specific isotope enrichment factors are needed for a quantitative assessment of in situ biodegradation in contaminated groundwater. to obtain information on the variability on carbon and hydrogen enrichment factors (epsilonc, epsilonh) the isotope fractionation of methyl tertiary (tert-) butyl ether (mtbe) and ethyl tert-butyl ether (etbe) upon aerobic degradation was studied with different bacterial isolates. methylibium sp. r8 showed a carbon and hydrogen isotope enrichment u ... | 2007 | 17410802 |
| characterization of two beta-carotene ketolases, crto and crtw, by complementation analysis in escherichia coli. | the pathways from beta-carotene to astaxanthin are crucial key steps for producing astaxanthin, one of industrially useful carotenoids, in heterologous hosts. two beta-carotene ketolases (beta-carotene 4,4'-oxygenase), crto and crtw, with different structure are known up to the present. in this paper, we compared the catalytic functions of a crto ketolase that was obtained from a marine bacterium rhodococcus erythropolis strain pr4, crto derived from cyanobacterium synechosistis sp. pcc6803, and ... | 2007 | 17415558 |
| [detection of rhodococcus equi by microbiological culture and by polymerase chain reaction in samples of tracheobronchial secretions of foals]. | the goal of the present study was to investigate whether new pcr-methods would improve diagnostic of r. equi. in a first step, sensitivity and specificity of the pcr-methods in respect to the"gold standard" microbiological culture were determined. secondly, sensitivity and specificity of both microbiological methods were evaluated in respect to the clinical diagnosis. the tracheobronchial secretions of 48 foals with pulmonary abscesses and of 37 healthy foals were evaluated by bacteriological cu ... | 2007 | 17416135 |
| characterization of a c-c bond hydrolase from sphingomonas wittichii rw1 with novel specificities towards polychlorinated biphenyl metabolites. | sphingomonas wittichii rw1 degrades chlorinated dibenzofurans and dibenzo-p-dioxins via meta cleavage. we used inverse pcr to amplify dxnb2, a gene encoding one of three meta-cleavage product (mcp) hydrolases identified in the organism that are homologues of bphd involved in biphenyl catabolism. purified dxnb2 catalyzed the hydrolysis of 8-oh 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (hopda) approximately six times faster than for hopda at saturating substrate concentrations. moreover, the speci ... | 2007 | 17416660 |
| discovery of a eugenol oxidase from rhodococcus sp. strain rha1. | a gene encoding a eugenol oxidase was identified in the genome from rhodococcus sp. strain rha1. the bacterial fad-containing oxidase shares 45% amino acid sequence identity with vanillyl alcohol oxidase from the fungus penicillium simplicissimum. eugenol oxidase could be expressed at high levels in escherichia coli, which allowed purification of 160 mg of eugenol oxidase from 1 l of culture. gel permeation experiments and macromolecular ms revealed that the enzyme forms homodimers. eugenol oxid ... | 2007 | 17419730 |
| characterization of two biphenyl dioxygenases for biphenyl/pcb degradation in a pcb degrader, rhodococcus sp. strain rha1. | rhodococcus sp. rha1 induces two biphenyl dioxygenases, the bpha and etba/ebda dioxygenases, during growth on biphenyl. their subunit genes were expressed in r. erythropolis iam1399 to investigate the involvement of each subunit gene in their activity and their substrate preferences. the recombinant expressing ebda1a2a3etba4 and that expressing bpha1a2a3a4 exhibited 4-chlorobiphenyl (4-cb) transformation activity, suggesting that these gene sets are responsible for the etba/ebda and bpha dioxyge ... | 2007 | 17420585 |
| improvement of dibenzothiophene desulfurization activity by removing the gene overlap in the dsz operon. | dibenzothiophene (dbt) and its derivatives can be microbially desulfurized by dsz enzymes. we investigated the expressional characteristics of the dsz operon. the result revealed that the ratio of mrna quantity of dsza, dszb, and dszc was 11:3.3:1; however, western blot analysis indicated that the expression level of dszb is far lower than that of dszc. gene analysis revealed that the termination codon of dsza and the initiation codon of dszb overlapped, whereas there was a 13-bp gap between dsz ... | 2007 | 17420595 |
| an aerobic sequencing batch reactor for 2,4,6-trinitrophenol (picric acid) biodegradation. | a bench scale sequencing batch reactor (sbr) was designed and tested for degradation of 2,4,6-trinitrophenol (tnp) or picric acid-contaminated wastewater or groundwater. under varying temperatures (25, 15 and 10 degrees c) and influent concentrations (40-200 mg/l tnp) a stable biomass was developed that was consistently capable of degrading the explosive compound to below regulatory drinking water limits (0.057 mg/l). the reactor was initially seeded with a nitroaromatic and nitramine degrading ... | 2007 | 17286267 |
| nitrile biotransformations for the efficient synthesis of highly enantiopure 1-arylaziridine-2-carboxylic acid derivatives and their stereoselective ring-opening reactions. | catalyzed by the rhodococcus erythropolis aj270 whole cell catalyst under very mild conditions, biotransformations of racemic 1-arylaziridine-2-carbonitriles proceeded efficiently and enantioselectively to produce highly enantiopure s-1-arylaziridine-2-carboxamides and r-1-arylaziridine-2-carboxylic acids in excellent yields. although the nitrile hydratase exhibits no selectivity against all nitrile substrates, the amidase is highly r-enantioselective towards 1-arylaziridine-2-carboxamides. when ... | 2007 | 17286438 |
| enrichment and isolation of endosulfan degrading and detoxifying bacteria. | in the present study, degradation of endosulfan by a mixed culture isolated from a pesticide-contaminated soil was studied in batch experiments. after two weeks of incubation, the mixed culture was able to degrade 73% and 81% of alpha and beta endosulfan respectively. endodiol was identified by gc/ms as degradation intermediate. the toxicity studies of endosulfan before and after degradation were carried out using micronucleus assay on human polymorphonuclear cells. the findings suggested that t ... | 2007 | 17289112 |
| enhancement of biodesulfurization in two-liquid systems by heterogeneous expression of vitreoscilla hemoglobin. | the vgb gene, encoding vitreoscilla hemoglobin (vhb), was introduced into a specific desulfurization bacterium, rhodococcus erythropolis lsse8-1. the vhb-specific spectrum was observed for the recombinant. compared to the wild type, the strain bearing vgb showed a higher biomass yield and desulfurizing activity. | 2007 | 17293512 |
| cutaneous pyogranuloma in a cat caused by virulent rhodococcus equi containing an 87 kb type i plasmid. | a 2-year-old intact male domestic shorthaired cat presented with a chronic, nodular, ulcerated, cutaneous lesion on the right thoracic limb. histological and cytological examination revealed a pyogranulomatous inflammation with basophilic organisms in the macrophages. a virulent form of rhodococcus equi containing an 87 kb type i (vapa) virulence plasmid was identified from cultures of biopsy samples. this report describes the clinicopathological features, plasmid profile and virulence of this c ... | 2007 | 17300450 |
| in vivo expression of and cell-mediated immune responses to the plasmid-encoded virulence-associated proteins of rhodococcus equi in foals. | rhodococcus equi is a facultative intracellular pathogen that causes pneumonia in foals but does not induce disease in adult horses. virulence of r. equi depends on the presence of a large plasmid, which encodes a family of seven virulence-associated proteins (vapa and vapc to vaph). eradication of r. equi from the lungs depends on gamma interferon (ifn-gamma) production by t lymphocytes. the objectives of the present study were to determine the relative in vivo expression of the vap genes of r. ... | 2007 | 17301216 |
| what is your diagnosis? rhodococcus equi. | 2007 | 17302546 | |
| oral administration of a live attenuated salmonella vaccine strain expressing the vapa protein induces protection against infection by rhodococcus equi. | rhodococcus equi remains one of the most important pathogens of foals and vaccination strategies to prevent rhodococcosis are under increasing investigation. attenuated salmonella strains carrying heterologous antigens offer an advantageous alternative to conventional vaccines, especially because they induce mucosal and systemic immunity. in this work, we expressed the vapa antigen from r. equi in a salmonella enterica typhimurium strain, which was able to colonize and persist in the lymphoid ti ... | 2007 | 17307012 |
| linalool production from the leaves of bursera aloexylon and its antimicrobial activity. | the oil of the leaves of bursera aloexylon was found to contain a high linalool level (96.7 %). the antimicrobial activity tests indicated that the oil was effective against rhodococcus equi (0.60 mg/ml) and staphylococcus epidermides (0.15 mg/ml). | 2007 | 17482377 |
| analysis of catrabc operon for catechol degradation from phenol-degrading rhodococcus erythropolis. | the gene cluster catrabc, involved in catechol degradation, was isolated from rhodococcus erythropolis ccm2595. the genes cata, catb, catc, and the divergently transcribed catr code for catechol 1,2-dioxygenase, cis,cis-muconate cycloisomerase, muconolactone isomerase, and an iclr-type transcriptional regulator, respectively. measurements of catechol 1,2-dioxygenase activity showed that the expression of cata is induced by phenol but not by catechol or cis,cis-muconate. the activity of catechol ... | 2007 | 17483937 |
| polychlorinated biphenyl/biphenyl degrading gene clusters in rhodococcus sp. k37, ha99, and ta431 are different from well-known bph gene clusters of rhodococci. | four kinds of polychlorinated biphenyl (pcb)-degrading rhodococcus sp. (ta421, ta431, ha99, and k37) have been isolated from termite ecosystem and under alkaline condition. the bph gene cluster involved in the degradation of pcb/biphenyl has been analyzed in strain ta421. this gene cluster was highly homologous to bph gene clusters in r. globerulus p6 and rhodococcus sp. rha1. in this study, we cloned and analyzed the bph gene cluster essential to pcb/biphenyl degradation from r. rhodochrous k37 ... | 2007 | 17485846 |
| [degradation of p-nitrophenol by a mangrove bacterial rhodococcus sp. ns]. | a bacterium capable of utilizing p-nitrophenol (pnp) as the sole nitrogen, carbon, and energy source was isolated from mangrove sediment. this bacterium was confirmed based on 16s rdna sequence analysis and taxonomic analysis as being a member of the genus rhodococcus and was designated strain ns. the strain ns could tolerate high concentrations of pnp up to 1.5 mmol/l and complete degradation was achieved in four days at 30 degrees c in the dark under aerobic conditions. biodegradation of pnp q ... | 2007 | 17489212 |
| transcriptional regulation of the virr operon of the intracellular pathogen rhodococcus equi. | the virr operon, located on the virulence plasmid of the intracellular pathogen rhodococcus equi, contains five genes, two of which (virr and orf8) encode transcriptional regulators. the first gene of the operon (virr), encoding a lysr-type transcriptional regulator, is transcribed at a constitutive low level, whereas the four downstream genes are induced by low ph and high growth temperature. differential regulation of the virr operon genes could not be explained by differential mrna stability, ... | 2007 | 17496078 |
| physiological, numerical and molecular characterization of alkyl ether-utilizing rhodococci. | twenty-seven gram-positive strains were characterized physiologically and numerically and classified them into four groups according to their specific activities for utilization of linear alkyl ethers (aes), cyclic aes, monoalkoxybenzenes and 1,4-diethoxybenzene. the comparative analysis of the 16s ribosomal rna gene and 16s-23s intergenic spacer region showed that they belonged to the genera rhodococcus and gordonia. alkyl ether-utilizing rhodococci appeared to involve various and diverse cytoc ... | 2007 | 17504487 |
| growth promotion of quorum-quenching bacteria in the rhizosphere of solanum tuberosum. | among 17 molecules structurally related to n-acylhomoserine lactone (nahl), gamma-caprolactone (gcl), 6-caprolactone (6cl) and 4-heptanolide (htn) were found to stimulate the degradation of nahl by bacterial communities recovered from bulk and rhizospheric soils. in the 6cl-, gcl- and htn-treated bacterial consortia, the nahl-degrading bacteria were more abundant than in control (mannitol-treated) consortia. moreover, the gcl- and htn-consortia showed a biocontrol activity against pectobacterium ... | 2007 | 17504488 |
| intradiol pathway of para-cresol conversion by rhodococcus opacus 1cp. | the growth of rhodococcus opacus 1cp in medium with different concentrations of p-cresol as the sole source of carbon and energy was studied. it was shown that the optimal concentration of p-cresol was 600 mg/l. the ability of this strain to transform practically all amounts of p-cresol to 4-methylcatechol followed by its utilization through ortho-pathway was shown. new enzymes (4-methylcatechol 1,2-dioxygenase, catechol 1,2-dioxygenase, and methylmuconate cycloisomerase) were purified to homoge ... | 2007 | 17506026 |
| assessment of three-dimensional biofilm structure using an optical microscope. | a method allowing the evaluation of the structure and the calculation of the volume of a biofilm, using an optical microscope, is proposed based on the linear relation between the intensity of a pixel in biofilm images grabbed on the x-y plane and the corresponding number of cells in the z direction, which allows the calculation of the biofilm thickness. the method is intended to overcome the need for expensive microscopes to study biofilms. | 2007 | 17515200 |
| biotransformation of d-methionine into l-methionine in the cascade of four enzymes. | d-methionine was converted to l-methionine in a reaction system where four enzymes were used. d-amino acid oxidase (d-aao) from arthrobacter protophormiae was used for the complete conversion of d-methionine to 2-oxo-4-methylthiobutyric acid. catalase was added to prevent 2-oxo-4-methylthiobutyric acid decarboxylation. in the second reaction step, l-phenylalanine dehydrogenase (l-phedh) from rhodococcus sp. was used to convert 2- oxo-4-methylthiobutyric acid to l-methionine, and formate dehydrog ... | 2007 | 17534960 |
| rhodococcus fascians infection accelerates progression of tobacco by-2 cells into mitosis through rapid changes in plant gene expression. | * to characterize plant cell cycle activation following rhodococcus fascians infection, bacterial impact on cell cycle progression of tobacco by-2 cells was investigated. * s-phase-synchronized by-2 cells were cocultivated with r. fascians and cell cycle progression was monitored by measuring mitotic index, cell cycle gene expression and flow cytometry parameters. cell cycle alteration was further investigated by cdna-aflp (amplified fragment length polymorphism). * it was shown that cell cycle ... | 2007 | 17547674 |
| [function analysis of the effective strain rhodococcus ruber em1 in wastewater treatment system by quantitative competitive pcr]. | a quantitative competitive pcr (qc-pcr) system was developed to quantify the number and analyze the function of the rhodococcus ruber em1 strain in a wastewater treatment system in nanchong oil refinery plant. strain em1 was able to degrade various kinds of hydrocarbons and aromatic compounds with high efficiency and produce bioemulsifier, so it was introduced into the waste liquid petroleum-disposing system. the sediment samples were collected from the disposing system in the range of 5 months, ... | 2007 | 17552240 |
| arabidopsis amidase 1, a member of the amidase signature family. | amidase 1 (ami1), a specific indole-3-acetamide amidohydrolase, is an arabidopsis thaliana amidase signature enzyme that catalyzes the synthesis of indole-3-acetic acid from indole-3-acetamide. amidase signature family members catalyze a diverse range of enzymatic reactions and are found widespread in nature, for instance in bacteria, mammals, and plants. at the protein level, the family members share a conserved stretch of approximately 50-130 amino acids, the name-giving amidase signature. elu ... | 2007 | 17555521 |
| monitoring source water for microbial contamination: evaluation of water quality measures. | watershed management programs often rely on monitoring for a large number of water quality parameters to define contaminant issues. while coliforms have traditionally been used to identify microbial contamination, these indicators cannot discriminate among potential contaminant sources. microbial source tracking (mst) can provide the missing link that implicates the sources of contamination. the objective of this study was to use a weight-of-evidence approach (land use analysis using gis, sanita ... | 2007 | 17560623 |
| immunization by intrabronchial administration to 1-week-old foals of an unmarked double gene disruption strain of rhodococcus equi strain 103+. | rhodococcus equi causes fatal granulomatous pneumonia in foals and immunocompromised animals and humans. however, there is no effective vaccine against this infection. in this study, the chromosomal genes isocitrate lyase (icl) and cholesterol oxidase (choe) were chosen as targets for mutation and assessment of the double mutant as an intrabronchial vaccine in 1-week-old foals. using a modification of a suicide plasmid previously developed in this laboratory, we developed a choe-icl unmarked del ... | 2007 | 17560744 |
| enzymatic reduction of ketones in "micro-aqueous" media catalyzed by adh-a from rhodococcus ruber. | mono- and biphasic aqueous-organic solvent systems (50% v v-1) as well as micro-aqueous organic systems (99% v v-1) were successfully employed for the biocatalytic reduction of ketones catalyzed by alcohol dehydrogenase adh-a from rhodococcus ruber via hydrogen transfer. a clear correlation between the log p of the organic solvent and the enzyme activity--the higher, the better--was found. the use of organic solvents allowed highly stereoselective enzymatic carbonyl reductions at substrate conce ... | 2007 | 17469836 |
| pharmacokinetics of oral doxycycline and concentrations in body fluids and bronchoalveolar cells of foals. | the objective of this study was to determine the disposition of orally administered doxycycline in foals. six healthy 4- to 8-week-old foals were used. doxycycline was administered to each foal via the intragastric (ig) route at dosages of 10 and 20 mg/kg, in a cross-over design. after the first 10 mg/kg dose, five additional doses were administered at 12-h intervals. a microbiological assay was used to measure doxycycline activity in serum, urine, peritoneal fluid, synovial fluid, cerebrospinal ... | 2007 | 17472649 |
| [immobilization of rhodococcus ruber strain gt1, possessing nitrile hydratase activity, on carbon sorbents]. | rhodococcus ruber strain gtl, possessing nitrile hydratase activity, was immobilized by adsorption on carbon supports differing in structure and porosity. the adsorption capacity of the supports towards cells, the substrate of the nitrile hydratase reaction (acrylonitrile), and the product (acrylamide) was studied. also, the effect of immobilization and nitrile hydratase activity of bacteria was investigated, and the operational stability of the immobilized biocatalyst was determined. it was sho ... | 2007 | 17476805 |
| biotransformation of heterocyclic dinitriles by rhodococcus erythropolis and fungal nitrilases. | 2,6-pyridinedicarbonitrile (1a) and 2,4-pyridinedicarbonitrile (2a) were hydrated by rhodococcus erythropolis a4 to 6-cyanopyridine-2-carboxamide (1b; 83% yield) and 2-cyanopyridine-4-carboxamide (2b; 97% yield), respectively, after 10 min. after 118 h, the intermediates 1b or 2b were transformed into 2,6-pyridinedicarboxamide (1c; 35% yield) and 2,6-pyridinedicarboxylic acid (1d; 60% yield) or 2-cyanopyridine-4-carboxylic acid (2c; 64% yield), respectively. the nitrilase from fusarium solani af ... | 2007 | 17479225 |
| a 40-year-old man with cough and fever. | 2007 | 17479943 | |
| an oil-degrading bacterium: rhodococcus erythropolis strain 3c-9 and its biosurfactants. | to isolate a biosurfactant-producing bacterium and find new products within its culture. | 2007 | 17578426 |
| [cerebral abscess by rhodococcus equi in a hiv-negative patient]. | rhodococcus equi is an unusual cause of infection in humans. infection in immunocompetent host is extremely rare. this report describes a case of 57 years old patient rhodococcus brain and pulmonary infection in a patient who did not have hiv or otherwise immunocompromised (either from disease, immunosuppressive medications, or both). he was admitted in the hospital due to neurological symptoms, and diagnosed after undergoing medical tests (brain ct scan and mr) with an intracranial neoplasia. n ... | 2007 | 17590093 |
| novel aspects of symbiotic (polyvinyl alcohol) biodegradation. | a new polyvinyl alcohol (pva)-degrading bacterium was isolated from activated sludge sampled during a waste water treatment process and identified as sphingomonas sp. its pva oxidase activity and alcohol dehydrogenase activity for various low-molecular-weight secondary alcohols were detected. both activities were associated with cells of the degrader, and they were not extracellular. under optimal conditions, the isolate was able to degrade 500 mg of pva per litre in 2 weeks. the strain required ... | 2007 | 17594087 |
| chronic granulomatous disease in pediatric patients: 25 years of experience. | chronic granulomatous disease (cgd) is an uncommon primary immune deficiency (affecting 1/200,000 newborn infants) caused by a defect in phagocyte production of oxygen metabolites, and resulting in bacterial infections produced by catalase-positive microorganisms and fungal diseases that occasionally may prove fatal. | 2007 | 17594870 |
| streptomyces turgidiscabies possesses a functional cytokinin biosynthetic pathway and produces leafy galls. | streptomyces turgidiscabies, a cause of potato scab, possesses a mobilizable pathogenicity island containing multiple virulence genes and a cytokinin biosynthetic pathway. these biosynthetic genes are homologous and collinear with the fas operon in rhodococcus fascians. reverse-transcriptase polymerase chain reaction of s. turgidiscabies demonstrated that all six genes were transcribed in oat bran broth with and without glucose, though transcription was partially repressed by glucose. the supern ... | 2007 | 17601163 |
| remarkable electronic and steric effects in the nitrile biotransformations for the preparation of enantiopure functionalized carboxylic acids and amides: implication for an unsaturated carbon-carbon bond binding domain of the amidase. | biotransformations of various functionalized racemic nitriles catalyzed by rhodococcus erythropolis aj270, a nitrile hydratase/amidase-containing microbial whole-cell catalyst, were studied. while the nitrile hydratase exhibits high catalytic efficiency but very low enantioselectivity against almost all nitrile substrates examined, the amidase is very sensitive toward the structure of the amides. the release of the steric crowdedness around the stereocenter of the substrates and the introduction ... | 2007 | 17604398 |
| purification and characterization of rhodobactin: a mixed ligand siderophore from rhodococcus rhodochrous strain ofs. | the siderophore produced by rhodococcus rhodochrous strain ofs, rhodobactin, was isolated from iron-deficient cultures and purified by a combination of xad-7 absorptive/partition resin column and semi-preparative hplc. the siderophore structure was characterized using 1d and 2d (1)h, (13)c and (15)n nmr techniques (dqfcosy, tocsy, noesy, hsqc and lr-hsqc) and was confirmed using esi-ms and ms/ms experiments. the structural characterization revealed that the siderophore, rhodobactin, is a mixed l ... | 2007 | 17273817 |
| mass spectrometry reveals the missing links in the assembly pathway of the bacterial 20 s proteasome. | the 20 s proteasome is an essential proteolytic particle, responsible for degrading short-lived and abnormal intracellular proteins. the 700-kda assembly is comprised of 14 alpha-type and 14 beta-type subunits, which form a cylindrical architecture composed of four stacked heptameric rings (alpha7beta7beta7alpha7). the formation of the 20 s proteasome is a complex process that involves a cascade of folding, assembly, and processing events. to date, the understanding of the assembly pathway is in ... | 2007 | 17430901 |
| continuous production of chiral 1,3-butanediol using immobilized biocatalysts in a packed bed reactor: promising biocatalysis method with an asymmetric hydrogen-transfer bioreduction. | an asymmetric hydrogen-transfer biocatalyst consisting of mutated rhodococcus phenylacetaldehyde reductase (par) or leifsonia alcohol dehydrogenase (lsadh) was applied for some water-soluble ketone substrates. among them, 4-hydroxy-2-butanone was reduced to (s)/(r)-1,3-butanediol, a useful intermediate for pharmaceuticals, with a high yield and stereoselectivity. intact escherichia coli cells overexpressing mutated par (sar268) or lsadh were directly immobilized with polyethyleneimine or 1,6-dia ... | 2007 | 17443321 |
| requirement of duplicated operons for maximal metabolism of phthalate by rhodococcus sp. strain dk17. | the operons encoding the transformation of phthalate to protocatechuate are duplicated and present on two different megaplasmids [pdk2 (330 kb) and pdk3 (750 kb)] in rhodococcus sp. strain dk17. rt-pcr experiments using gene-specific primers showed that both the pdk2- and the pdk3-encoded dihydroxyphthalate decarboxylase genes are simultaneously expressed during growth on phthalate. the doubling time of the pdk2-cured mutant strain dk176 in minimal liquid medium with 5mm phthalate is 52.5% of th ... | 2007 | 17449009 |
| bacterial decolorization of acid orange 7 in the presence of ionic and non-ionic surfactants. | the effects of the non-ionic surfactant triton x-100, the cationic surfactant cetyltri-methylammonium bromide (ctab) and the anionic surfactant sodium n-lauroyl sarcosinate (sls) on the decolorization of the reaction medium containing the monoazo dye acid orange 7 (ao7) by alcaligenes faecalis and rhodococcus erythropolis were studied. it was found that the surfactants influenced in different ways the rate of decolorization. at all concentrations tested the non-ionic surfactant triton x-100 decr ... | 2007 | 17425112 |
| plasmid vectors for testing in vivo promoter activities in corynebacterium glutamicum and rhodococcus erythropolis. | novel shuttle promoter-probe vectors replicating in escherichia coli, corynebacterium glutamicum, and rhodococcus erythropolis were constructed on the basis of the c. glutamicum plasmid pcg1. the vectors carry reporter genes coding for fluorescent proteins, which allow the measurement of promoter activities in vivo. the promoter-probe vector ppre11 contains the rsgfp reporter gene, coding for a variant of green fluorescent protein (gfp) with a red-shifted excitation maximum. to ensure efficient ... | 2007 | 17657537 |
| domain formation by a rhodococcus sp. biosurfactant trehalose lipid incorporated into phosphatidylcholine membranes. | the study of the interaction of biosurfactants with biological membranes is of great interest in order to gain insight into the molecular mechanisms of their biological actions. in this work we report on the interaction of a bacterial trehalose lipid produced by rhodococcus sp. with phosphatidylcholine membranes. differential scanning calorimetry measurements show a good miscibility of the glycolipid in the gel state and immiscibility in the fluid state, suggesting domain formation. these domain ... | 2007 | 17662234 |
| [lignite microorganisms]. | the first demonstration that samples of lignite at a depth of 10 m are considerably enriched in bacteria is reported. according to direct microscopy, the abundance of bacteria was about 10(7) cells/g. about 70% of cells had intact cell membranes and small size, which points to their anabiotic state. the fungal mycelium length was no more than 1 m. lignite inoculation onto solid glucose-yeast-peptone medium allowed as to isolate bacteria of the genera bacillus, rhodococcus, arthrobacter, micrococ ... | 2007 | 17668464 |