Publications

[the mode of action of bacteriocin n5 purified from clostridium perfringens].	the purified bacteriocin n5 produced by clostridium perfringens bp6k inhibited simultaneously the syntheses of dna, rna and protein in sensitive cells, without dna degradation. bacteriocin n5 inhibited the accumulation of leucin and caused the exit of the previously accumulated amino acid. the effects of bacteriocin n5 are very similar to those observed for colicins e1, k, a and i.	1975	177225
[hygienic evaluation of the finding of the causative agents of food poisoning in food products].		1975	180687
[effect of antibiotics on the resistance of white mice to c1. perfringens toxin].	the effect of potassium benzylpenicillin, streptomycin sulfate and chlortetracycline on experimental gangrenous intoxication was studied. when the antibiotics were injected before intoxication, the albino mice resistance did not significantly change. when the antibiotics were injected immediately after the toxin administration, the resistance of the test animals markedly decreased.	1975	180880
clostridium perfringens exotoxins. iii. binding of theta-toxin to erythrocyte membrane.	when clostridium perfringens theta-toxin was incubated with sheep erythrocytes the toxin activity disappeared before lysis, the fact of which suggests fixation of the toxin to erythrocyte membranes. 2. theta-toxin lost its activity by binding to cell membranes, and the membrane constituted inhibitor of theta-hemolysis was neither a protein, a carbohydrate nor a phosphatide, but was cholesterol. from these results this report proposes that the theta-toxin binding site of erythrocytes should be ch ...	1975	183032
[studies on intra- and interspecies relationships of bacterial hemolysins. 1. identification of marked and masking toxin relationships of cl. perfringens. efficiency of the serum-unmasking test for the diagnosis of the classical c-subtype].		1975	178124
[sensitivity of pathogenic clostridia to antibiotics].	by the sensitivity levels of the gas infection causative agents, i. e. pathogenic clostridia to antibiotics, the latter were conditionally divided into 4 groups. the 1st group included the most active antibiotics, such as tetracyclines,, penicillins, cephalosporins, rifampicin, 7-chlor-7-desoxylincomycin. their minimum inhibitory and bactericidal concentrations did not usually exceed 2 gamma/ml. for most of the strains the inhibitory and bactericidal concentrations amounted to the tenth and hund ...	1975	178272
[bacteriological studies on alimentary egg paste. hygienic and sanitary aspects of the problem].		1975	179481
[principle and new media for sporulation of clostridium perfringens. preliminary results with an attenuated mutant].		1975	178082
experience with a new agar culture medium for demonstrating the presence of anaerobic bacteria.	in view of the fact that anaerobic diagnostics have become a matter of primary importance, the authors have developed the rcm medium with certain modifications. owing to its good keeping quality, this medium can be used also on smaller laboratories for demonstrating the presence of anaerobic bacteria and for carrying out germ counts. it constitutes and adequate base for the anaerobic blood medium and for the wilson-blair medium, and also for the determination of the antibiotic resistance of the ...	1975	194528
flat bed gel isoelectric focusing of neuraminidases.		1975	234196
grain overload in cattle and sheep: changes in microbial populations in the cecum and rumen.	samples from the rumen and cecum of cattle and sheep were cultured to determine changes in microbial populations resulting from overfeeding with grain. before the animals were overfed, the predominant organisms from both sides were those that grew anaerobically on a relatively nonselective ruminal fluid medium and would not grow on selective mediums designed to culture lactobacilli, streptococci, coliforms, or clostridium perfringens. by 24 hours after overfeeding, lactic acid bacteria had incre ...	1975	234213
1-alkyl-2-nitroimidazol-5-yl derivatives. i.		1975	235267
effect of lysozyme on ionic forms of spores of clostridium perfringens type a.	h spores of clostridium perfringens type a (two strains) were more sensitive to germination by lysozyme than native spores. resistance to lysozyme of h spores was restored by calcium loading.	1975	236284
isoelectric focusing in thin layer polyacrylamide gel combined with a zymogram method for detecting enzyme microheterogeneity: sample application.		1975	236690
[ornithine transcarbamylase in clostridium perfingens].	five strains of a type cl. perfringens of different titres of toxicity (bp6k, sr-12, 2836, 2910, 1) possessed a distinct activity of ornithine transcarbamylase. in cells the maximal activity of the enzyme was observed within 3-5 hrs of growth of the culture. the enzyme was partially purified and some of its physico-chemical properties were studied. ornithine transcarbamylase was termostable. monoiodine acetate, p-chloromercurybenzoate, semicarbazide, hg-2+, cu-2+ and fe-3+ inhibited the enzymati ...	1975	237365
[carbonic acid in the metabolism of bacteria of the genus clostridium].		1975	237586
nutritional requirements for growth and sporulation of clostridium perfringens.		1975	237860
preparation of a glycoprotein fraction from pooled human plasma and its evaluation as a substrate for the assay of clostridium welchii (c. perfringens) neuraminidase.	a glycoprotein fraction (fraction vii) suitable for use as a substrate in assays of microbial neuraminidase was prepared from pooled human plasma. it is pasteurised during preparation to eliminate the risk of transmission of serum hepatitis. this results in polymerisation of some of the gamma1-acid glycoprotein, but fraction vii is shown to be an excellent substrate for the neuraminidase of clostridium welchii (c. perfringens). a sensitive assay procedure is described. a number of factors may in ...	1975	238036
reactions of fluorescent probes with normal and chemically modified myelin.	the fluorescent probes 8-anilino-1-naphthalenesulfonate (ans) and 2-p-toluidinylnaphthalene-6-sulfonate (tns) bind to highly purified myelin membranes obtained from bovine brain white matter. binding of the dyes was markedly increased by environmental conditions which reduce the negative surface potential of the membrane, i.e., cations (la-3+ is greater than ca-2+ is greater than na-+,k-+), h-+, local anesthetics, and the antibiotic polymyxin b. chemical alteration of accessible membrane charged ...	1975	238581
the relationship between different forms of human alpha-mannosidase.	the tissue distribution and some properties of human alpha-mannosidase (alpha-d-mannoside mannohydrolase ec 3.2.1.24) have been studied. the acidic forms of the enzyme were fairly stable, whereas the neutral forms easily lost enzymic activity. the acidic forms were sensitive to neuraminidase but the neutral forms were unaffected. the experiments indicate that the acidic components are closely related to each other, differing only in sialic acid content and possibly conformation. the neutral form ...	1975	238600
purification and characterization of guinea pig lymphotoxin produced by lymph node cells stimulated by phytohemagglutinin.	guinea pig lymphotoxin (lt) produced by stimulation of lymph node cells with phaseolus vulgaris phytohemagglutinin was purified approximately 1,000-fold in specific activity by ammonium sulfate fractionation, deae-sephadex column chromatography, and gel filtration on sephadex g-100. the properties of lt tested at the various stages of purification revealed that the lt was a heat-sensitive, protease-sensitive proteinaceous substance, and its approximate molecular weight was estimated to be 50,000 ...	1975	239474
evaluation of bacteriological transport systems.	seven commercially manufactured bacteriological transport systems, including culturette, trans-cul (with stuart and amies), handiswab, securline (with amies and amies without charcoal) and culture caddy, were evaluated to determine whether these systems were capable of maintaining the viability and constant numbers of mixtures of hardy, fastidious and anaerobic organisms over 72 hours at 25c and 4c. the results of the study indicated that if specimens are maintained in transport systems at 25c t ...	1975	239597
asparagine-linked oligosaccharide chains of igg: a revised structure.		1975	239717
1-alkyl-2-nitroimidazol-5-yl derivatives ii. n-substituted nitrones.		1975	239726
regulation of breakdown and synthesis of l-glutamate decarboxylase in clostridium perfringens.	l-glutamate decarboxylase (gad) activity of clostridium perfringens (atcc 8009) cells grown in various culture conditions was investigated. remarkable variations of gad level occur during the growth cycle in thioglycollate broth. these changes are affected by the ph of the culture medium. addition of alkali to the culture media results in decrease of cell gad activity, whereas increase of enzyme level occurs only in cells growing in unbuffered media. the results indicate that the mechanism regul ...	1975	239920
cytotoxic contaminants in commercial clostridium perfringens neuraminidase preparations purified by affinity chromatography.		1975	239961
[michaelis constants of neuraminidases of pathogenic and apathogenic microorganisms (author's transl)].	the km-values of neuraminidases from different pathogenic and apathogenic microorganisms have been determined on low and high molecular substrates. the substrate specificity and the affinity to the different types of substrates in relation to the pathogenicity of the microorganisms are discussed.	1975	241169
public health importance of clostridium perfringens.		1975	241737
hematological findings in acute infections and septicemias.	this paper is a report on twelve cases of septicemia, the diagnosis made possible by medical technologists observing the degenerative morphological findings in the peripheral blood smears. this led to the finding of microorganisms, prior to the bacteriological confirmation, in an active emergency department. it also includes four case reports in which the organism was identified: a meningococcus, a pneumonococcus, an escherichia coli, and a clostridium perfringens (welchii).	1975	242218
endo-beta-n-acetylglucosaminidases acting on carbohydrate moieties of glycoproteins: purification and properties of the two enzymes with different specificities from clostridium perfringens.		1975	242275
responses of clostridium botulinum type b and e progenitor toxins to some clostridial sulfhydryl-dependent proteases.	sulfhydryl-dependent proteases produced by clostridium botulinum types a, b, and f, clostridium histolyticum, clostridium sporogenes and clostridium perfringens activate preferentially type e over type b progenitor toxin but less efficiently than trypsin. the results explain why activable toxin is demonstrable in culture of a strongly proteolytic type b strain.	1975	1104932
clostridium septicemia following biliary surgery in a gastrectomized patient.	a 75-year-old woman was subjected to biliary surgery 38 years after partial gastrectomy for ulcer. there was a history of gallstones of 10 years duration, pentagastrin-resistant achylia, cholecystolithiasis and choledocholithiasis complicated by stenosis of papilla of vater, cholecystitis and pancreatitis. peroperative cholangiography and biliary tract surgery were performed. on the third postoperative day heavy jaundice and hemolysis developed, leading to death of the patient. culture of bile t ...	1975	1124339
purification of two clostridium bacteriocins by procedures appropriate to hydrophobic proteins.	two clostridocins distinguishable by their different modes of action on clostridium pasteurianum have been isolated, namely, butyricin 7423 found in cultures of clostridium butyricum ncib 7423 and perfringocin 11105 produced by clostridium perfringens type a, ncib 11105. both were trypsin-susceptible proteins which were soluble in concentrated aqueous ethanol and were able to bind large amounts of the nonionic detergent triton x-100. in the presence of triton x-100, butyricin 7423 behaved as a h ...	1975	1137378
[purification and chemical study of a collocalia glycoprotein].	a glycoprotein was purified from the aqueous extract of "edible bird's nest" (collocalia) using free flow preparative electrophoresis and represented the main fraction of collocalia glycoproteins. this glycoprotein is homogeneous upon agarose electrophoresis and slightly polydisperse upon ultracentrifugation (s so 20w = 3,0). the carbohydrate moiety contains galactose, mannose, glucosamine, galactosamine and sialic acid, which is completely released by clostridium perfringens or diplococcus pneu ...	1975	1182216
the reduction of n-hydroxy-4-acetylaminobiphenyl by the intestinal microflora of the rat.	the role of the intestinal flora in the conversion of n-hydroxy-4-acetyl-aminobiphenyl (n-oh-aabp) to 4-acetylaminobiphenyl has been examined. this reaction, which reverses the metabolic activation of the parent carcinogen, can be demonstrated in cultures of some bacteria indigenous to the intestinal microflora. these include cultures of clostridium sp., clostridium perfringens, peptostreptococcus productus i, and bacteroides fragilis ss. thetaiotaomicron and ss. vulgatus. in contrast, cultures ...	1975	1182690
antigenic structure of wild clostridium perfringens strain type a and its mutants.		1975	1212625
characterization of two glycoasparagines isolated from the urine of patients with aspartylglycosylaminuria (agu).	two major glycoasparagines (2-acetamido-n-(4'-l-aspartyl)-2-deoxy-beta-d-glycosylamines) were isolated from the urine of patients with aspartylglycosylaminuria (agu). they were composed of equimolar amounts of sialic acid, galactose, glucosamine, and aspartic acid. they were isomeric with respect to the position of sialic acid attachment, since they produced the same glycoasparagine on incubation with the neuraminidase from clostridium perfringens. the structure of the resulting sialic acid-free ...	1975	1213985
isolation of clostridium in human infections: evaluation of 114 cases.	one hundred fifty-two strains of clostridium were isolated from 144 patients over a 14-month-peroid. these included 23 recognized species and 23 strains that were unclassified. soft tissues or abscesses yielded 84 strains of clostridium. intraabdominal sites predominated, but clostridia were recovered from empyema, carcinoma, frostbite with gas gangrene, muscle abscess, aortic graft, and brain abscess. blood cultures yielded 65 strains of clostridium from 49 patients, representing 0.3% of 16,314 ...	1975	805193
competitive effects of intestinal microflora on vibrio cholerae in gnotobiotic mice.	the coexistence of vibrio cholerae and several intestinal bacteria was determined in gnotobiotic mice. the bacteria tested included a bacteroides sp, clostridium difficile, clostridium perfringens type a, 2 separate isolates of escherichia coli, 2 different lactobacilli, 2 separate isolates of proteus mirabilis, pseudomonas maltophilia, and streptococcus faecalis. each species of bacteria became established and was recovered repeatedly from the stools during the studies. no single strain or spec ...	1975	807775
[normal status of bac. cereus and cl. perfringens in the body of healthy slaughter animals].	studied were cattle, pigs, sheep, and young calves intended for slaughter. the experiments were carried out under productional conditions, strictly observing the routinely adopted practice of preslaughter handling. the blood of the animals was sampled prior to slaughter. samples from the meat (musculature), spleen, kidneys, liver, mesenterial and body lymph nodes as well as feces were taken immediately after slaughter. it was established that bacillus cereus and clostridium perfringens were almo ...	1976	816051
the effect of phospholipase c on human blood platelets.	the effect of phospholipase c (ec 3.1.4.3) on human blood platelets has been studied. phospholipase c from bacillus cereus was purified to homogeneity as judged by analytical and sodium dodecyl sulphate disc gel electrophoresis and by immunoelectrophoresis. human platelets isolated from platelet-rich plasma by gel filtration or by centrifugation and washing were incubated with phospholipase c. a loss of 20-45% of the total platelet phospholipid was observed, whereas 88% was hydrolyzed when plate ...	1976	819457
clostridium perfringens infection complicating chemotherapy for choriocarcinoma.	a case of clostridium perfringens sepsis and gas gangrene complicating chemotherapy for gestational choriocarcinoma is reported. the infection was eradicated using antibiotics, surgery, and hyperbaric oxygen therapy. the pathophysiology, diagnosis, and treatment of this unusual but often lethal complication are reviewed.	1976	943077
anaerobic infections in hospital practice.	the role of anaerobes in the pathogenesis of infections was investigated. anaerobes were isolated from 0,25% of blood cultures and from 15,8% of other specimens tested; in 15,1% of cases where anaerobes were isolated, no aerobes were found. the strains most commonly encountered were bacteroides fragilis, b. melaninogenicus, peptostreptococcus anaerobius and clostridium perfringens. sensitivity tests in vitro showed all organisms to be sensitive to clindamycin, metronidazole and chloramphenicol, ...	1976	973160
[gas gangrene panophthalmitis (author's transl)].	gas gangrene panophthalmitis is a rare condition of penetrating injury to the globe. the infecting organism is usually clostridium perfringens. characteristic symptoms are a brawny swelling of the lids, marked chemosis, coffee-coloured discharge, hypopyon, ring abscess of the cornea, formation of gas bubbles in the anterior chamber, rise of intraocular tension and early amaurosis. treatment consists in the evisceration or enucleation of the globe, rarely in the exenteration of the orbit. antibio ...	1976	979027
[studies on the composition of the fecal flora of healthy dogs with the special references of lactobacillus flora and bifidobacterium flora (author's transl)].	with the new method the fecal flora of 13 clinically healthy dogs aged 3 to 42 months was analysed qualitatively and quantitatively. it was characterized by that bacteroidaceae constituted the most prodominant flora, catenabacteria, streptococci, peptostreptococci, lactobacilli and bifidobacteria were the next most numerous, enterobacteria consisted the accompanying flora. the numbers of clostridium perfringens were remarkably fluctuated and seem to be influenced by the composition of the food i ...	1976	983534
histone redistribution and conformational effect on chromatin induced by formaldehyde.	histone redistributions between endogenous dna in calf thymus chromatin and exogenous dna from clostridium perfringens (69% a + t) or from micrococcus luteus (30% a + t) induced by 0.6 m nacl or by 2% formaldehyde were studied by thermal denaturation. the observed redistribution occurred on histone hl when the exogenous dna was (a + t)-richer than the dna in chromatin, and when the mixture was exposed to 0.6 m nacl or formaldehyde. when a (g + c)-richer dna was added as the acceptor for histones ...	1976	987800
pathophysiology in the acute afferent loop syndrome. a study in rats.	the afferent loop syndrome, i.e. occlusion of an afferent intestinal loop after a billroth ii partial gastrectomy, was induced in rats. after various time intervals the animals were killed and the haematocrit and serum osmolality were determined. the content of the occluded loop was analysed with respect to volume, bacterial flora and osmolality. in some cases a sample of the content was incubated at 37 degrees c and the osmolality determined at regular intervals. groups of animals were studied ...	1976	1007793
the importance of clostridia in experimental intestinal strangulation.	hemorrhagic and ischemic strangulation of closed intestinal segments was sutdied in germ-free rats individually monocontaminated with one of eight separate species of clostridia. ischemic strangulation was found to be as deadly as hemorrhagic strangulation in the presence of clostridia. some clostridia were very toxic whereas others were relatively innocuous. the clostridia, ranked in the order of decreasing lethality, were: clostridium perfringens type a, c. septicum, c. histolyticum, c. haemol ...	1976	1085711
isolation and identification of anaerobic bacteria.	anaerobic bacteria make up a predominant part of the normal human flora. adequate specimen collection must avoid contamination with this flora. suitable methods include thoracentesis, transtracheal aspiration, needle and syringe aspiration of closed abscesses, and endocervical aspiration of intrauterine pus. swabs are generally unsuitable. sputum, voided urine, vaginal secretions, and specimens contaminated with feces are not cultured anaerobically. specimens should be transported in an oxygen-f ...	1976	770306
a modified plate holding system for anaerobic cultures.	a modified plate holding system for anaerobes has been developed which: 1) effectively reduces the volume of oxygen-free gas required, 2) promotes earlier bacterial growth, and 3) monitors the anaerobic atmosphere of the holding vessel and the rate of gas flow. cultures of bacteroides fragilis, clostridium perfringens, c. septicum, fusobacterium nucleatum, peptococcus sp. and propionibacterium sp. were evaluated for optimal growth. all cultures grew as well as or better when preincubated in the ...	1976	782243
a rapid glutamic decarboxylase test for identification of bacteria.	a simple rapid glutamic decarboxylase test is described. this test was found useful in the identification of escherichia coli, shigella sp., providencia alcalifaciens, clostridium perfringens and bacteroides fragilis.	1976	793504
properties of ecto-(inoganic) pyrophosphatase of nervous system cells in culture. activation upon partial release of sialic acid from the cell surface.	clonal line nn hamster astroblasts and clonal line n18 neuroblasts were treated with phospholipase c-free, protease-free, and hemolysin-free clostridium perfringens sialidase, at a low level (5 x 10(-3) units/ml) so as to maintain cell intactness and to avoid spurious protein effects. a rapid, regular release of sialic acid was achieved. an approximately 9-fold increase in ecto-pyrophosphatase activity could be brought about by action of c. perfringens sialidase for 10 min. since the sialidase p ...	1976	1245485
cytotoxic effects on the plasma membrane of human diploid fibroblasts--a comparative study of leakage tests.	confluent monolayers of human diploid lung fibroblasts were treated with cytolytic agents. the induced membrane damage was investigated by different test systems. changes of membrane permeability were compared with morphological alterations. four tests employed leakage of cytoplasmic markers of different sizes as criteria of membrane damage. radioactive markers of the following decreasing size order were used: [3h]rna (mw greater than 200,000) greater than 51cr greater than [3h]nucleotides great ...	1976	1263610
pellet-gun brain wound complicated by clostridium perfringens meningitis.	a ten-year-old male was hospitalized for a pellet-gun wound to the brain. he developed clostridial meningitis within eighteen hours in spite of radical debridement and prophylactic antibiotics. however, successful recovery was obtained with high levels of penicillin and chloromycetin antibiotic therapy.	1976	1265638
the possible welchihin activity of clostridium perfringens type a enterotoxin.		1976	190663
[identification of two plasmids isolated from a bacteriocinogenic strain of clostridium perfringens].	two plasmids were found and studied in the bacteriocinogenic strain n5 of clostridium perfringens: one is a bacteriocinogenic factor (mw=5.7 x 10(6)) and the other a cryptic plasmid (mw 32.4 x 10(6)). simultaneous loss of the ability to produce bacteriocin and of the bacteriocin resistance in a "cured" variant corresponds to the loss of the bacteriocinogenic plasmid dna. the syntheses of haemolysin 0 and phospholipase c do not seem to be coded for by the cryptic plasmid.	1976	190933
[experimental reproduction of necrotic enteritis in the chicken. 1. mono- and polyinfections with clostridium perfringens and coccidia with reference to cage managemen].	experimental mono-infection and poly-infection, using vegetative germs of a cl. perfringens type a strain 1663 and its toxin as well as e. acervulina, necatrix or mitis oocysts, were applied to 100 spf chicken aged seven days. while clostridial or coccidial mono-infection did not cause any loss and only unspecific pathologic-anatomic changes, polyinfection was accompanied by diarrhoea and slower weight increase, with 55.4 per cent of the chicken having been lost three to nine days from the outbr ...	1976	190968
[experimental reproduction of necrotic enteritis in the chicken. 2. further mono- and polyinfections with cl. perfringens and coccidia with special reference to ground-kept chickens].	coccidial oocysts and/or vegetative germs, spores, and toxins of cl. perfringens type a were used in mono-infection and poly-infection experiments on spf chicken aged seven and 56 days and kept under different conditions. necrotic enteritis was regularly reproduced in all experimental groups with polyinfections. in chicken aged seven days necrotic enteritis was reproduced even after repeated mono-infections with 4 x 10(9) gerus and toxin of cl. perfringens. the loss figures recorded from ground- ...	1976	190969
effects of concanavalin a and neuraminidase on cyclic amp levels and 14c-1-glucose oxidation in dog thyroid slices.	treatment with concanavalin a at 100 micron/ml or higher concentrations significantly increased 14c-1-glucose oxidation in dog thyroid slices as reported in other tissues. this treatment exerted no effect on tissue cyclic amp levels. neuraminidase at the same concentrations also had similar effects on these parameters. neither concanavalin a nor neuraminidase at the concentrations up to 100 microng/ml had the tsh effect on both tissue cyclic amp and 14c-1-glucose oxidation. these results indicat ...	1976	191247
[characteristics of the proteolytic complex of clostridium perfringens type a].		1976	191802
[ovine enterotoxemia caused by cl. perfringens type a].		1976	191981
[experience in treating sheep with clostridial infections].		1976	191982
[purification and some properties of "clostridium perfringens" delta toxin (author's transl)].	delta toxin, a hemolytic exocellular protein excreted by c. perfringens type c has been purified to homogeneity, assessed by polyacrylamide disc gel electrophoresis. purification steps involved successively calcium phosphate gel formation in culture supernatant fluid, salting-out of unadsorbed material by ammonium sulfate to 50 % saturation, isoelectric focusing and gel filtration on sephadex g75. purified toxin appears as a basic protein occuring in two forms with isoelectric points of 8.8 and ...	1976	192118
aerobic and anaerobic flora in bladder urine of patients with indwelling urethral catheters.	twenty-five bladder urine specimens from 13 patients with long-standing indwelling catheters were obtained by percutaneous suprapubic needle aspiration to avoid contamination by extraneous flora and cultured aerobically and anaerobically. twenty different species of organism were isolated from twenty-four positive specimens. the isolates were predominantly gram-negative aerobic or facultatively anaerobic bacilli, with occasional gram-positive aerobic cocci and yeasts. one patient had clostridium ...	1976	178084
"phoenix phenomenon" in the growth of clostridium perfringens.	the "phoenix phenomenon" was observed with clostridium perfringens hobbs' serological type 9 (ht9) in a cooked-meat medium at 81.7 degrees c by a decrease in plate count (phase i), followed by an increase in count to the intiial level (phase ii) and a continued increase above the initial count (phase iii). the effects of sporulation, age of inoculum, assay medium, anaerobiosis, diluent, and growth inhibitor were studied. this phenomenon was reproduced in experiments with sporulation-negative mut ...	1976	188384
[ultrastructure of micropredators of gram-positive bacilli and the degeneration of c perfringens].		1976	188562
[properties of ionic forms of spores of a clostridium perfringens].		1976	189102
in vitro aggregation of platelets induced by alpha-toxin (phospholipase c) of clostridium perfringens.	highly purified alpha-toxin (phospholipase c) of clostridium perfringens prepared by affinity chromatography on agarose-linked egg-yolk lipoprotein induced the in vitro aggregation of platelets of an irreversible type. the aggregation started after a time lag, the length of which depended on the concentration of the toxin; the reciprocal of the time lag was found to be directly proportional to the toxin concentration. using this assay method, we demonstrated that the platelet-aggregating activit ...	1976	189107
[immunologic indicators in patients with suppurative processes in the abdominal cavity].		1976	189120
parasitic nematode larvae as agents of transmission of pathogenic bacteria.		1976	189513
[use of a jet injector for administration of sorbed typhoid vaccine with polytoxoid].		1976	189534
[researches on the water quality of the river po and its tributaries between cremona and casalmaggiore. i. materials and methods (author's transl)].	during 1971 a research program about the status of pollution of the river po and its tributaries was started by six institutes of hygiene altogether (turin, pavia, milan, parma, modena and ferrara universities) with the institute for water research of c.n.r. and hydrographic office of magistracy of the po. the role of the parma university hygiene institute was to study the water qualities of the river po between cremona and casalmaggiore and of two right side tributaries, arda-ongina and taro. t ...	1976	189776
[changes in the electrical activity of the cerebral cortex under the combined influence of cl. perfringens type a toxin and products of cl. butyricum activity].	a study of the changes of the electrocorticogram (ecog) and the electrocardiogram (ecg) in combined action of cl. perfringens, type a, and of the cl. butyricum broth culture filtrate showed that desynchronization of the cortical electrical activity and its subsequent depression occurred at earlier periods than in the case of isolated administration of cl. perfringens toxin. the general character of the changes in the cortical rhythmic activity remained the same as in intoxication caused by cl. b ...	1976	189855
temperate phages of clostridium perfringens type c1.	four phages isolated from carrier strains of clostridium perfringens type c belong to two classes. the three phages of class i, c1, c3, and c4, and homoimmune and serologically closely related. the phage of class ii, c5, is heteroimmune to the class i phages and not related to them serologically. transduction experiments with several of the phages were negative. mutants of the indicator strain with surface alterations occurred spontaneously in stock cultures. electron micrographs show the phages ...	1976	179685
action of clostridium perfringens neuraminidase on gangliosides gm1 and gm2 above and below the critical micelle concentration of substrate.		1976	179891
letter: cephaloridine penetration of bone.		1976	179971
clostridium perfringens exotoxins. iv. inhibition of the theta-toxin induced hemolysis by steroids and related compounds.	1. the inhibitory powers of thirty two samples of steroids and their related compounds on the theta-toxin induced hemolysis were assayed. apparent i50 value of cholesterol, the most potent inhibitor amoung them, was 0.022 mum. 2. inhibitory powers of sterol acetates were not more than one tenth of those of the corresponding sterol, and steroids without 3-hydroxyl group hardly inhibited the hemolysis. these results suggest that 3-hydroxyl group of sterols plays an important role in the inhibition ...	1976	180313
evolution of the digestive microflora in a unit of specified-pathogen-free mice: efficiency of the barrier.	rhoico strain], barrier-maintained since 1970, is described. some "contaminants" appeared spontaneously during the period 1971-1973, but microorganisms belonging to the genera lactobacillus streptococcus, enterobacteria, escherichia and bacillus remained stable. the methods of investigation used were not suitable for the assessment of strictly anaerobic strains. the stability of the digestive microflora durine the last 2 years of this study is believed to be related to the skill and conscientiou ...	1976	180327
[clostridium perfringens infection. problems posed excluding resuscitation].	the authors report 18 cases of clostridium perfringens infection and discuss the main clinical and etiological aspects apart from gynecological forms and those usually requiring intensive care. pathological foci, digestive infection and the problem of bacteremia are discussed together with appropriate treatment.	1976	188174
[the interspecies masking between staphylococcus pyogenes and clostridium perfringens toxins].		1976	188273
[study of leukotoxic activity of cl. perfringens type a toxin and its fractions obtained by gel filtration and ion exchange chromatography].	in gel-filtration of cl. perfringens type a toxin on sephadex f-100 and f-50 there was revealed relationship between the leukotoxic activity and a high-molecular component. a method of ion-exchange chromatography on a column with deae-sephadex a-25 from the cl. perfringens toxin there were obtained 8 fractions 3 of which possessed a marked leukotoxic activity: the capacity to destroy neutrophils in the svejcar and vancurik test and to-depress the phagocytic activity of leukocytes. lecithinase an ...	1976	188277
metronidazole bioassay.	urine from patients receiving metronidazole [1-(2-hydroxyethyl)-2-methyl-5-nitromidazole] orally or per vagina were subjected to paper chromatographic fractionation and examined for anticlostridial activity. unmodified metronidazole and several metabolites were detected. these findings indicate that the clostridial bioassay may be limited in its applicability to the study of the pharmacodynamics of metronidazole as it does not differentiate between the parent substance and some of its metabolite ...	1976	178274
effect of delayed evisceration on the microbial quality of meat.	the postomortem invasion of muscle and other tissues by bacteria from the intestinal tract was studied with the use of radioactive tracers. the injection of 14c-labeled bacteria or spores into the intestines of guinea pig carcasses within 24 h of death resulted in the rapid spread of 14c throughout carcasses. when live bacteria were injected along with the labeled cells, it was not possible to isolate viable organisms from the body tissues if the living animal had been exposed to the bacteria. i ...	1976	178276
inhibition by zinc of hemolysis induced by bacterial and other cytolytic agents.	zinc, cupric, and cadmium ions, in that order of effectiveness, inhibited lysis of washed, rabbit erythrocytes by the toxic bacterial product aerolysin. hemolysis induced by a variety of other lytic agents was also inhibited by zn2+ in approximately the same concentration as that, 0.33 mm, needed to inhibit aerolysin-induced hemolysis. zinc ions did not inhibit osmotic lysis. inhibition requires the continues presence of zn2+ and apparently involves a readily reversible binding of zn2+ to the ce ...	1976	178604
collaborative study of an improved method for the enumeration and confirmation of clostridium perfringens in foods.	a collaborative study was conducted in 10 laboratories to evaluate the performance of a new method for the enumeration of vegetative cells of clostridium perfringens in foods. results obtained by the new method were compared with results from the official first action method, 46.049-46.053. per cent recoveries of 4 c. perfringens strains from inoculated roast beef samples were higher and more consistent in tryptose-sulfite-cycloserine (tsc) agar with or without added egg yolk than in sulfite-pol ...	1976	178636
[symptomatology of diseases caused by c1. perfringens].		1976	178949
[letter: acute non-lithiasic welchia perfringens cholecystitis. an unusual cause of pneumoperitoneum].		1976	179057
bacillus cereus food poisoning.		1976	179147
[about the influence of beta-toxin of clostridium perfringens (type c) on the motorics of intestine segments (in vitro). i. communication].	the beta-toxin of clostridium perfringens (type c) was introduced intra lumen to jejunum and ileum segments of rabbits, then examined by pharmacologic method (in vitro). the beta-toxin showed a paralysing activity on the motorics of the intestine which fact may be in relation to the paralysing activity of this toxin in dysenteria in piglets.	1976	179254
[study of c1. perfringens resistance to lincomycin].	a possibility of developing resistant forms of c1. perfringens during treatment of experimental anaerobic (gaseous) infection with lincomycin was studied. it was shown that treatment of the animals for 7 days resulted in an increase in the resistance by 33-41 times. it was noted that strains with decreased sensitivity to lincomycins had changed morphology and biochemical activity (decreased lecitinase activity, changed biochemical properties), decreased virulence and pathogenicity for animals. s ...	1976	179463
[changes in the electric activity of the cerebral cortex in poisoning caused by cl. perfringens toxin type a].	the electrical activity of the cerebral cortex was recorded in cats under mild nembutal anesthesia (15-20 mg/kg of body weight) during the development of cl. perfringens, type a, toxin poisoning (the toxin was injected intramuscularly in a dose of 100 mld per kg of body weight). two phases of the changes in the electrical activity of the cerebral cortex were noted. the first phase was attended by the desynchronization of the electrical activity, persistence of the induced potentials and of the r ...	1976	196701
antibody formation in mice in various genotypes after immunization with highly purified perfringens alpha-toxoid.		1976	199137
[demonstration and type determination of clostridium perfringens isolated from meat semipreserves].	investigated were a total of 92 samples of pasteurized cans of different batches. six strains of clostridium perfringens were isolated from a sample of pasteurized ham. to demonstrate a toxin and present its type differentiation a strain was investigated with the use of dermonecrotic test after williams along with the cross toxin neutralization after the internationally accepted method, and the neutralization of the lecithinase activity after williams. the use of native toxins at the type differ ...	1976	193241
[alpha-toxin (phospholipase c) of clostridium perfringens (author's transl)].		1976	194279
[properties and hemolytic action of clostridium perfringens theta-toxin (author's transl)].		1976	194280
[complementation of theta-and kappa-toxin production between two group mutants of clostridium perfringens (author's transl)].		1976	194281
production and properties of theta-toxin of clostridium perfringens with special reference to lethal activity.	theta-toxin of clostridium perfringens produced in a synthetic medium showed high toxicity. the mouse was killed with 5-10 hemolytic units of the toxin. neutralization experiments showed that lethal and hemolytic activities were due to the same toxic entity. the amount of theta-toxin expressed in lethal activity reached more than 30% that of alpha-toxin in the synthetic medium sm67. although the activities of alpha-and theta-toxins were not additive in terms of ld50, increase in the ratio of the ...	1976	192930
[prevalence of clostridium perfringen (welchii) in the bovine udder].		1976	204967
[necrotizing enteritis and geophagia].	two cases of intestinal perforation due to necrotising enteritis in patients with pica are presented. the aetiopathogenesis of pica is discussed as well as its role in the development of necrotising enteritis.	1976	183183
[further experimental investigations on the therapy of gas gangrene with ozone and oxygen (author's transl)].	in continuation of a recently-published investigation on the effect of intramuscular injections of an ozone-oxygen mixture on experimental gas gangrene in the guinea pig, 2 additional experiments were performed in order to establish the efficacy of the therapy when given twice daily instead of only once. futhermore, the effect of an analogous therapy was investigated with the use of pure oxygen. the ozone-oxygen therapy reduced the death rate from 100% in untreated control animals to 73.6 and 70 ...	1976	183374