Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| ai-2 quorum-sensing inhibitors affect the starvation response and reduce virulence in several vibrio species, most likely by interfering with luxpq. | the increase of disease outbreaks caused by vibrio species in aquatic organisms as well as in humans, together with the emergence of antibiotic resistance in vibrio species, has led to a growing interest in alternative disease control measures. quorum sensing (qs) is a mechanism for regulating microbial gene expression in a cell density-dependent way. while there is good evidence for the involvement of auto-inducer 2 (ai-2)-based interspecies qs in the control of virulence in multiple vibrio spe ... | 2009 | 19778962 |
| effect of antimicrobial agents on the toll-like receptors and inflammatory cytokines in liver tissue of the alcohol-induced liver disease in rats with vibrio vulnificus sepsis. | septicemia and inflammation-mediated septic shock caused by vibrio vulnificus (vv) is strongly associated with chronic liver disease. this study examined the effects of antimicrobial therapy on expression of hepatic toll-like receptors and inflammatory cytokines in rats with alcohol-induced liver disease complicated by vv sepsis. | 2009 | 19781370 |
| the extracellular metalloprotease of vibrio tubiashii directly inhibits its extracellular haemolysin. | vibrio tubiashii is a re-emerging pathogen of molluscs that secretes a variety of extracellular products (ecps), including a metalloprotease and a cytolysin/haemolysin. previously, we reported that the v. tubiashii haemolysin locus consists of two orfs (vthb and vtha), similar to that of the homologous haemolysin genes (vvhb and vvha) found in vibrio vulnificus. here, we demonstrate that the concomitant expression of both v. tubiashii genes resulted in significantly higher haemolytic activity th ... | 2009 | 19389767 |
| an amphioxus tlr with dynamic embryonic expression pattern responses to pathogens and activates nf-kappab pathway via myd88. | a big bang expansion of the vertebrate-type (v-type) tlrs was reported in amphioxus. to shed lights on its implications, a unique tlr which is reversely inserted into an intron of amphioxus psmb7-10 by retrotransposition in the highly polymorphic proto-mhc region was cloned from chinese amphioxus (branchiostoma belcheri tsingtauense) and named as bbttlr1. in situ assays showed that bbttlr1 was predominantly expressed in pharynx and gut from larva to adult stages, which are considered as the firs ... | 2009 | 19394698 |
| dynamic expressions of liver tissue apoptosis-related genes of vibrio vulnificus sepsis rats and the effects of antibacterial agents. | dynamic changes in mrna expressions of liver tissue apoptosis-promoting genes fas and bax and apoptosis-inhibiting gene bcl-2 of vibrio vulnificus sepsis rats were detected and the effects of antibacterial agents were examined. the rat model with vibrio vulnificus sepsis (vv group) was established and some of the vibrio vulnificus sepsis rats were treated with antibacterial agents (aa group). the mrna expressions of fas, bax and bcl-2 were measured by reverse transcription polymerase chain react ... | 2009 | 19399403 |
| comparison of the effects of deferasirox, deferiprone, and deferoxamine on the growth and virulence of vibrio vulnificus. | since deferoxamine (dfo), a standard iron-chelating agent that is widely used in patients with iron overload such as hemochromatosis or thalassemia, is a kind of hydroxamine siderophore of streptomyces species, it can accelerate the in vitro growth of ferophilic organisms such as vibrio vulnificus, yersinia enterocolitica, and mucorales. | 2009 | 19413741 |
| role of calcium/calmodulin signaling pathway in vibrio vulnificus cytolysin-induced hyperpermeability. | endothelial hyperpermeability, a hallmark of septicemia, is induced by stress fiber formation, which is primarily regulated by the calcium/calmodulin signaling pathway in endothelial cells. we previously reported that trifluoperazine, a calcium/calmodulin antagonist, blocks vibrio vulnificus cytolysin (vvc) -induced lethality at in vivo animal model. the object of this study was therefore to examine whether vvc induces stress fiber formation through calcium/calmodulin signaling in endothelial ce ... | 2009 | 19447166 |
| vibrio vulnificus produces quorum sensing signals of the ahl-class. | vibrio vulnificus is an aquatic pathogenic bacterium that can cause vibriosis in humans and fish. the species is subdivided into three biotypes with the fish-virulent strains belonging to biotype 2. the quorum sensing (qs) phenomenon mediated by furanosyl borate diester or autoinducer 2 (ai-2) has been described in human strains of biotype 1, and here we show that the luxs gene which encodes ai-2 is present in all strains of v. vulnificus regardless of origin, biotype or serovar. in this study, ... | 2009 | 19453744 |
| the capability of catabolic utilization of n-acetylneuraminic acid, a sialic acid, is essential for vibrio vulnificus pathogenesis. | n-acetylneuraminic acid (neu5ac, sialic acid) could provide a good substrate for enteropathogenic bacteria in the intestine, when the bacteria invade and colonize in human gut. in order to analyze the role of neu5ac catabolism in vibrio vulnificus pathogenesis, a mutant with disruption of the nana gene encoding neu5ac lyase was constructed by allelic exchanges. the nana mutant was not able to utilize neu5ac as a sole carbon source and revealed an altered colony morphotype with reduced opacity in ... | 2009 | 19487477 |
| [effects of antimicrobial agents on the toll like receptors and myeloid differentiation protein-2 in liver tissue of alcohol-induced liver disease: experiment with rats]. | to investigate the effects of antimicrobial agents on the toll like receptors (tlrs) and myeloid differentiation protein (md)-2 in liver tissue of alcohol-induced liver disease with vibrio vulnificus (vv) sepsis. | 2009 | 19489280 |
| user friendly cloning coupled with chitin-based natural transformation enables rapid mutagenesis of vibrio vulnificus. | vibrio vulnificus is a bacterial contaminant of shellfish and causes highly lethal sepsis and destructive wound infections. a definitive identification of virulence factors using the molecular version of koch's postulates has been hindered because of difficulties in performing molecular genetic analysis of this opportunistic pathogen. for example, conjugation is required to introduce plasmid dna, and allelic exchange suicide vectors that rely on sucrose sensitivity for counterselection are not e ... | 2009 | 19502446 |
| septic shock from penetrating leg injury with vibrio vulnificus infection. | 2009 | 19527215 | |
| benthic ecology of vibrio spp. and pathogenic vibrio species in a coastal mediterranean environment (la spezia gulf, italy). | we carried out a 16-month in situ study to investigate the ecology of vibrio spp. and pathogenic vibrio species in coastal sediments of the mediterranean sea, employing multiple-regression analysis to reveal the major environmental factors controlling their occurrence in the benthic environment. in addition, association between vibrios and sediment-inhabiting meiofauna, which is a major component of benthic ecosystems, was investigated. culturable and total vibrio spp. estimates by most-probable ... | 2009 | 19543938 |
| infections in patients with aplastic anemia. | infection is a major cause of death in patients with aplastic anemia (aa). there are differences between the immunocompromised state of a patient with aa and the patient who is neutropenic due to chemotherapy and this leads to a difference in the infections that they incur. prolonged neutropenia is one of the largest risk factors for the development of infections with the invasive mycoses and bacteria. recovery from neutropenia is directly related to survival, and supportive care plays a large r ... | 2009 | 19549579 |
| comparative evolutionary analysis of the major structural subunit of vibrio vulnificus type iv pili. | type iv pili contribute to virulence in vibrio vulnificus, the bacterium responsible for the majority of fatal seafood-related infections. here, we performed within- and between-species evolutionary analysis of the gene that encodes the major structural subunit of the pilus, pila, by comparing it with pild and gyrb, the genes encoding the type iv prepilin peptidase and beta subunit of dna gyrase, respectively. although the diversity in pild and gyrb is similar to each other and likely to have ac ... | 2009 | 19556347 |
| roles of rseb, sigmae, and degp in virulence and phase variation of colony morphotype of vibrio vulnificus. | vibrio vulnificus is an estuarine bacterium capable of causing serious and often fatal wound infections and primary septicemia. we used alkaline phosphatase insertion mutagenesis to identify genes necessary for the virulence of this pathogen. one mutant had an in-frame fusion of 'phoa to the gene encoding rseb, a periplasmic negative regulator of the alternative sigma factor sigma(e). sigma(e) controls an extensive regulon involved in responding to cell envelope stresses. colonies of the rseb mu ... | 2009 | 19564391 |
| incidence of vibrio vulnificus in estuarine waters of the south texas coastal bend region. | to determine the occurrence of the human pathogen, vibrio vulnificus, in south texas coastal waters. | 2009 | 19566724 |
| iron differentially regulates gene expression and extracellular secretion of vibrio vulnificus cytolysin-hemolysin. | in a previous study, we showed that vibrio vulnificus is a ferrophilic bacterium that requires high levels of available iron for growth. in the present study, we show that iron stimulates, in an unusual manner, the production of cytolysin-hemolysin (vvha), the most potent exotoxin produced by v. vulnificus. the vvha gene possesses a putative ferric uptake regulator (fur)-binding box in the regulatory region, andvvha transcription was repressed by iron and de-repressed by fur mutation. however, e ... | 2009 | 19586415 |
| septic arthritis as the initial manifestation of fatal vibrio vulnificus septicemia in a patient with thalassemia and iron overload. | vibrio vulnificus infection is an uncommon but potentially fatal disease in children such that prompt recognition has prognostic implications. we describe here the case of a 9-year-old female with thalassemia and iron overload who presented with septic arthritis as an atypical initial manifestation of fatal v. vulnificus septicemia. this report underscores the possibility of septic arthritis as an early manifestation of v. vulnificus septicemia. pediatricians should be alert to this extremely in ... | 2009 | 19591224 |
| integrase-directed recovery of functional genes from genomic libraries. | large population sizes, rapid growth and 3.8 billion years of evolution firmly establish microorganisms as a major source of the planet's biological and genetic diversity. however, up to 99% of the microorganisms in a given environment cannot be cultured. culture-independent methods that directly access the genetic potential of an environmental sample can unveil new proteins with diverse functions, but the sequencing of random dna can generate enormous amounts of extraneous data. integrons are r ... | 2009 | 19596808 |
| the fragment structure of a putative hsdr subunit of a type i restriction enzyme from vibrio vulnificus yj016: implications for dna restriction and translocation activity. | among four types of bacterial restriction enzymes that cleave a foreign dna depending on its methylation status, type i enzymes composed of three subunits are interesting because of their unique dna cleavage and translocation mechanisms performed by the restriction subunit (hsdr). the elucidated n-terminal fragment structure of a putative hsdr subunit from vibrio vulnificus yj016 reveals three globular domains. the nucleolytic core within an n-terminal nuclease domain (ntd) is composed of one ba ... | 2009 | 19625490 |
| in vitro and in vivo antimicrobial activity of water-soluble chitosan oligosaccharides against vibrio vulnificus. | vibrio vulnificus is a gram-negative bacterium that induces severely rapid pathological progress. in this study, we evaluated the antibacterial activity of two water-soluble chitosan oligosaccharides, cos a (mw, 10,000 da) and cos b (mw, 1,000 da), from 90-95% deacetylated chitosan, against v. vulnificus in vitro and in vivo. treatment with cos a resulted in significantly higher suppressive effects on the growth of v. vulnificus than treatment with cos b. the growth of v. vulnificus was inhibite ... | 2009 | 19639224 |
| calcium is essential for the major pseudopilin in the type 2 secretion system. | the pseudopilus is a key feature of the type 2 secretion system (t2ss) and is made up of multiple pseudopilins that are similar in fold to the type 4 pilins. however, pilins have disulfide bridges, whereas the major pseudopilins of t2ss do not. a key question is therefore how the pseudopilins, and in particular, the most abundant major pseudopilin, gspg, obtain sufficient stability to perform their function. crystal structures of vibrio cholerae, vibrio vulnificus, and enterohemorrhagic escheric ... | 2009 | 19640838 |
| phase variation, capsular polysaccharide, pilus and flagella contribute to uptake of vibrio vulnificus by the eastern oyster (crassostrea virginica). | vibrio vulnificus infections are associated with raw oyster consumption, and disease reservoirs are determined by the ability of this bacterium to infect and persist in oysters. surface structures, such as capsular polysaccharide (cps), pili and flagella, function as virulence factors in mouse infection models. furthermore, virulence is related to phase variation in colony morphology, which reflects cps expression and includes opaque (encapsulated, virulent), translucent (reduced encapsulation, ... | 2009 | 19689704 |
| structure of a polysaccharide from the lipopolysaccharides of vibrio vulnificus strains cect 5198 and s3-i2-36, which is remarkably similar to the o-polysaccharide of pseudoalteromonas rubra atcc 29570. | high-molecular-mass polysaccharides were released by mild acid degradation of the lipopolysaccharides of two wild-type vibrio vulnificus strain, a flagellated motile strain cect 5198 and a non-flagellated non-motile strain s3-i2-36. studies by sugar analysis and partial acid hydrolysis along with (1)h and (13)c nmr spectroscopies showed that the polysaccharides from both strains have the same trisaccharide repeating unit of the following structure: -->4)-beta-d-glcpnac3nacylan-(1-->4)-alpha-l-ga ... | 2009 | 19695563 |
| rigorous vibrio vulnificus soft tissue infection of the lower leg in a renal transplant patient managed by vacuum therapy and autologous growth factors. | vibrio vulnificus is a gram-negative marine bacterium that grows well in coastal waters. it is an opportunistic pathogen that can cause serious life-threatening infections in patients with certain health conditions. vibrio-induced wound infections in immunosuppressed patients are difficult to treat because the healing process may be significantly delayed. reconstructive surgery may not be successful in early treatment as skin grafts are likely to fail, and there may be increased morbidity of don ... | 2009 | 19706229 |
| knowledge of vibrio vulnificus infection among japanese patients with liver diseases: a prospective multicenter study. | vibrio vulnificus (v. vulnificus) is a seafood-borne infectious pathogen that can be lethal to humans. the infection has been correlated with pre-existing liver disease, particularly liver cirrhosis. awareness of v. vulnificus infection among japanese citizens is low, despite the increasing number of patients with hepatocellular carcinoma (hcc). the present study was conducted to assess the level of knowledge of patients with liver disease regarding v. vulnificus infection. | 2009 | 19789520 |
| evaluation of dna colony hybridization and real-time pcr for detection of vibrio parahaemolyticus and vibrio vulnificus in postharvest-processed oysters. | the applicability of real-time pcr was examined for detection of vibrios from postharvest-processed (php) oysters to allow for a more rapid assay and higher sample throughput than currently used. during june to october 2004, 68 php oyster samples were collected directly from php firms or from retail markets across the united states. php oysters were examined to determine the effectiveness of treatments in the reduction of vibrio levels and to compare the analytical methods utilized. the latter i ... | 2009 | 19833033 |
| survey of postharvest-processed oysters in the united states for levels of vibrio vulnificus and vibrio parahaemolyticus. | from june through october 2004, the u.s. food and drug administration collected oysters (61 samples) that had been subjected to postharvest processing (php) methods, including mild heat treatment, freezing, and high hydrostatic pressure, from processors and retail markets in various states to determine vibrio vulnificus and v. parahaemolyticus levels. presence in a 25-g sample and most probable number (mpn) using standard enrichment and selective isolation procedures were utilized. suspect colon ... | 2009 | 19833034 |
| tropical dermatology: marine and aquatic dermatology. | dermatoses caused by marine organisms are frequently seen in dermatology clinics worldwide. cutaneous injuries after exposure to marine environments include bacterial and fungal infections and lesions caused by aquatic plants and protists. some of these diseases are well known by dermatologists, such as vibrio vulnificus septicemia and erysipeloid, but others are uncommon, such as envenomation caused by ingestion or contact with certain dinoflagellates or cyanobacteria, which are associated with ... | 2009 | 19836641 |
| identification of the vibrio vulnificus ahpcl gene and its influence on survival under oxidative stress and virulence. | pathogens have evolved sophisticated mechanisms to survive oxidative stresses imposed by host defense systems, and the mechanisms are closely linked to their virulence. in the present study, ahpcl, a homologue of escherichia coli ahpc encoding a peroxiredoxin, was identified among the vibrio vulnificus genes specifically induced by exposure to h(2)o(2). in order to analyze the role of ahpcl in the pathogenesis of v. vulnificus, a mutant, in which the ahpcl gene was disrupted, was constructed by ... | 2009 | 19851736 |
| vibrio vulnificus infection: a case infected by contaminated fishing gear. | 2009 | 19878399 | |
| cadaverine is transported into vibrio vulnificus through its cadb in alkaline environment. | the exogenously-added cadaverine is effective in protecting vibrio vulnificus from methyl viologen (mv)-induced superoxide stress at ph 8.5. such a protective effect by cadaverine was not observed at ph 7.5. consistently, the accumulated level of intracellular cadaverine at ph 8.5 is approximately four times as much as that of the control cell at ph 7.5. cadaverine accumulation is not affected by mv. the protection of v. vulnificus by cadaverine from superoxide stress was abolished when cadb cod ... | 2009 | 19884768 |
| oligomerization is essential for apoptotic activity of vibrio vulnificus hemolysin. | vibrio vulnificus hemolysin (vvh), a pore forming toxin, is thought to be a virulence factor of this bacterium. it is well known that vvh induces apoptosis as well as cell lysis in susceptible target cells. although pore formation is an essential step in cell lysis, it is unknown whether this step is necessary for vvh-induced apoptosis. in this study, chinese hamster ovary (cho) cells were exposed to non-oligomerized mutant f334i, in which phenylalanine 334 was replaced by isoleucine. the rate o ... | 2009 | 19887751 |
| [development of taqman real-time pcr in detection of aeromonas hydrophila]. | to develop a taqman real-time pcr for the detection of aeromonas hydrophila. | 2009 | 19954074 |
| thrombocytopenia and thrombosis in disseminated intravascular coagulation (dic). | disseminated intravascular coagulation (dic) is the physiologic result of pathologic overstimulation of the coagulation system. despite multiple triggers, a myriad of laboratory abnormalities, and a clinical presentation ranging from gross hemostatic failure to life-threatening thrombosis, or even both simultaneously, a simplified clinical approach augmented by a few readily available tests allows prompt identification of the process and elucidation of treatment opportunities. platelet counts in ... | 2009 | 20008204 |
| [vibrio vulnificus infection outside of the fish ponds in northern israel: acquisition in the "einot tzukim" springs near the dead sea]. | vibrio vulnificus is a gram-negative bacterium that may cause severe skin and systemic infection after exposure of open wounds to contaminated water, especially in patients with underlying disease such as immune-deficiency, iron overload or end stage liver or renal disease. the v. vulnificus infection has been reported in israel almost exclusively after exposure to tilapia fish cultivated in fresh water fish ponds in northern israel. the authors report the first case of v. vulnificus infection a ... | 2009 | 20027981 |
| [real-time pcr in rapid diagnosis of aeromonas hydrophila necrotizing soft tissue infections]. | we report a case of rapidly progressive necrotizing soft tissue infection and sepsis followed by a patient's death. we suspected vibrio vulnificus infection because the patient's underlying disease was cirrhosis and the course extremely rapid. no microbe had been detected at death. we extracted dna from a blood culture bottle. sybr green i real-time pcr was conducted but could not detect v. vulnificus vvh in the dna sample. aeromonas hydrophila was cultured and identified in blood and necrotized ... | 2009 | 20034324 |
| expression, crystallization and preliminary x-ray diffraction analysis of a modification subunit of a putative type i restriction enzyme from vibrio vulnificus yj016. | modification (hsdm) and specificity (hsds) subunits are constituents of an active methyltransferase (mtase) of multifunctional type i restriction enzymes. to provide a molecular background on hsdm, a putative hsdm gene from vibrio vulnificus yj016 (hsdm_vv) was cloned and the expressed protein was purified and crystallized from 22%(w/v) polyethylene glycol 8000, 0.02 m imidazole ph 7.5 and 5 mm beta-mercaptoethanol. diffraction data were collected to 1.86 a resolution using synchrotron radiation ... | 2009 | 20054126 |
| dietary medicinal plant extracts improve growth, immune activity and survival of tilapia oreochromis mossambicus. | the effects of supplementing diets with acetone extract (1% w/w) from four medicinal plants (bermuda grass cynodon dactylon, h(1), beal aegle marmelos, h(2), winter cherry withania somnifera, h(3) and ginger zingiber officinale, h(4)) on growth, the non-specific immune response and ability to resist pathogen infection in tilapia oreochromis mossambicus were assessed. in addition, the antimicrobial properties of the extract were assessed against vibrio alginolyticus, vibrioparahaemolyticus, vibri ... | 2009 | 20735646 |
| necrotizing soft-tissue infections and primary sepsis caused by vibrio vulnificus and vibrio cholerae non-o1. | vibrio species are a rare cause of necrotizing soft-tissue infections and primary septicemia, which are likely to occur in patients with hepatic disease, diabetes, adrenal insufficiency, and immunocompromised conditions. these organisms thrive in warm seawater and are often present in raw oysters, shellfish, and other seafood. this study examined fulminating clinical characteristics of vibrio vulnificus and vibrio cholerae non-o1 soft-tissue infections and identified outcome predictors. | 2009 | 19276771 |
| characterization of a new plasmid-like prophage in a pandemic vibrio parahaemolyticus o3:k6 strain. | vibrio parahaemolyticus is a common food-borne pathogen that is normally associated with seafood. in 1996, a pandemic o3:k6 strain abruptly appeared and caused the first pandemic of this pathogen to spread throughout many asian countries, america, europe, and africa. the role of temperate bacteriophages in the evolution of this pathogen is of great interest. in this work, a new temperate phage, vp882, from a pandemic o3:k6 strain of v. parahaemolyticus was purified and characterized after mitomy ... | 2009 | 19286788 |
| hlyu acts as an h-ns antirepressor in the regulation of the rtx toxin gene essential for the virulence of the human pathogen vibrio vulnificus cmcp6. | in vibrio vulnificus, hlyu upregulates the expression of the large rtx toxin gene. in this work we identified the binding site of hlyu to -417 to -376 bp of the rtxa1 operon transcription start site. lacz fusions for a series of progressive deletions from the rtxa1 operon promoter showed that transcriptional activity increased independently of hlyu when its binding site was absent. thus hlyu must regulate the rtxa1 operon expression by antagonizing a negative regulator. concomitantly we found th ... | 2009 | 19320834 |
| temperature effects on the depuration of vibrio parahaemolyticus and vibrio vulnificus from the american oyster (crassostrea virginica). | this study investigated temperature effects on depuration for reducing vibrio parahaemolyticus and vibrio vulnificus in american oyster (crassostrea virginica). raw oysters were inoculated with 5-strain cocktail of v. parahaemolyticus or v. vulnificus to levels of 10(4) to 10(5) mpn (most probable number)/g and depurated in artificial seawater (asw) at 22, 15, 10, and 5 degrees c. depuration of oysters at 22 degrees c had limited effects on reducing v. parahaemolyticus or v. vulnificus in the oy ... | 2009 | 19323759 |
| localization and characterization of vva0331, a 489-kda rtx-like protein, in vibrio vulnificus yj016. | vibrio vulnificus yj016 contains three genes encoding proteins homologous to repeats-in-toxin proteins. one of these genes, vva0331, possesses a long open reading frame of 13,971 bp in length and resides on the small chromosome between two gene clusters encoding a type i secretion system and several regulatory proteins, respectively. bioinformatic analysis revealed that vva0331 consist of nineteen 87-amino acid repeats, two arg-gly-asp motifs, four cysteine residues, an outer membrane protein do ... | 2009 | 19326097 |
| immune response and inhibition of bacterial growth by electrotransfer of plasmid dna containing the antimicrobial peptide, epinecidin-1, into zebrafish muscle. | bacterial infections represent serious diseases in aquaculture, rapidly leading to fish death by septicemia. we investigated whether the electrotransfer of green fluorescent protein gene fusion epinecidin-1 (cmv-gfp-epi) dna into zebrafish muscle could regulate the fish immune response and inhibit bacterial growth. electroporation parameters such as the number of pulses, voltage, and amount of plasmid dna were analyzed, and results demonstrated the greatest mrna expression level of gfp-epi relat ... | 2009 | 19340938 |
| [necrotizing fasciitis caused by a vibrio vulnificus infection in an eel fishery]. | 2009 | 19348100 | |
| hemochromatosis and vibrio vulnificus wound infections. | there are several reports of persons with hemochromatosis and vibrio vulnificus primary septicemia, but few accounts of persons with hemochromatosis and v. vulnificus wound infection. a 58-year-old white man developed infection of a forearm injury exposed to seawater in the gulf of mexico near the alabama coast. at age 66, he was diagnosed to have hemochromatosis. transferrin saturation was 89% and serum ferritin was 4761 pmol/l. hfe genotype was c282y/c282y. serum levels of hepatic enzymes, glu ... | 2009 | 19349902 |
| [vibrio species in the waters of southern sweden caused bath-wound fever. increased bacteria frequency according to studies on clams]. | 2009 | 19350770 | |
| structure of a polysaccharide from the lipopolysaccharide of vibrio vulnificus clinical isolate yj016 containing 2-acetimidoylamino-2-deoxy-l-galacturonic acid. | a polysaccharide isolated after mild acid degradation of the lipopolysaccharide of vibrio vulnificus clinical isolate yj016 was found to contain l-fuc, d-glcpnac, 2,4-diacetamido-2,4,6-trideoxy-d-glucose (di-n-acetylbacillosamine, d-quinac4nac), and 2-acetimidoylamino-2-deoxy-l-galacturonic acid (l-galnama). the last sugar derivative was confirmed by correlations for nitrogen-linked protons in 2d tocsy and roesy spectra measured in a h(2)o-d(2)o mixture. the following structure of the polysaccha ... | 2009 | 19368903 |
| detection and enumeration of vibrio vulnificus by direct colony immunoblot. | a direct colony immunoblot method (dci) for the enumeration of vibrio vulnificus was developed. bacterial colonies were transferred from agar plates to membranes, which were then dried and blocked with bovine serum albumin. subsequently, the membranes were treated with anti-v. vulnificus h antibodies, washed and incubated with peroxidase-conjugated goat anti-rabbit igg. after a final wash, the membranes were exposed to a substrate mixture containing h(2)o(2) which resulted in the development of ... | 2009 | 19200106 |
| in vivo gene expression of cold shock and other stress-related genes in vibrio vulnificus during shellstock temperature control conditions in oysters. | to determine vibrio vulnificus response to shellstock refrigeration conditions while the bacterium was embedded in oysters (in vivo). | 2009 | 19200328 |
| plasmid diversity in vibrio vulnificus biotypes. | vibrio vulnificus is a heterogeneous bacterial species that can be virulent for humans and fish. virulence in fish seems to rely on a recently described plasmid that can be transmitted between strains, aided by a conjugative plasmid. the main objective of this work was to analyse the plasmid content of a wide collection of strains from the three biotypes of the species, as well as to identify putative conjugative and virulence plasmids by means of southern hybridization with specific probes and ... | 2009 | 19202097 |
| effects of flash freezing, followed by frozen storage, on reducing vibrio parahaemolyticus in pacific raw oysters (crassostrea gigas). | this study investigated the effects of flash freezing, followed by frozen storage, on reducing vibrio parahaemolyticus in pacific raw oysters. raw pacific oysters were inoculated with a five-strain cocktail of v. parahaemolyticus at a total level of approximately 3.5 x 10(5) most probable number (mpn) per gram. inoculated oysters were subjected to an ultralow flash-freezing process (-95.5 degrees c for 12 min) and stored at -10, -20, and -30 degrees c for 6 months. populations of v. parahaemolyt ... | 2009 | 19205481 |
| crystal structures of substrate and inhibitor complexes of ribose 5-phosphate isomerase a from vibrio vulnificus yj016. | ribose-5-phosphate isomerase a (rpia) plays an important role in interconverting between ribose-5-phosphate (r5p) and ribulose-5-phosphate in the pentose phosphate pathway and the calvin cycle. we have determined the crystal structures of the open form rpia from vibrio vulnificus yj106 (vvrpia) in complex with the r5p and the closed form with arabinose-5-phosphate (a5p) in parallel with the apo vvrpia at 2.0 a resolution. vvrpia is highly similar to eschericihia colirpia, and the vvrpia-r5p comp ... | 2009 | 19214439 |
| reduction of vibrio vulnificus in pure culture, half shell and whole shell oysters (crassostrea virginica) by x-ray. | the purpose of this investigation was to study the inactivation effect of x-ray treatments on inoculated vibrio vulnificus in pure culture, half shell and whole shell oysters to achieve a 5.0 log reduction, which is recommended by the interstate shellfish sanitation conference and the food and drug administration. a mixed culture of three v. vulnificus strains was used to prepare the pure culture and inoculated oysters. the pure culture and inoculated oysters were treated with 0.0, 0.1, 0.5, 0.7 ... | 2009 | 19217681 |
| spontaneous quinolone resistance in the zoonotic serovar of vibrio vulnificus. | this work demonstrates that vibrio vulnificus biotype 2, serovar e, an eel pathogen able to infect humans, can become resistant to quinolone by specific mutations in gyra (substitution of isoleucine for serine at position 83) and to some fluoroquinolones by additional mutations in parc (substitution of lysine for serine at position 85). thus, to avoid the selection of resistant strains that are potentially pathogenic for humans, antibiotics other than quinolones must be used to treat vibriosis o ... | 2009 | 19218407 |
| vibrio biofilms: so much the same yet so different. | vibrios are natural inhabitants of aquatic environments and form symbiotic or pathogenic relationships with eukaryotic hosts. recent studies reveal that the ability of vibrios to form biofilms (i.e. matrix-enclosed, surface-associated communities) depends upon specific structural genes (flagella, pili and exopolysaccharide biosynthesis) and regulatory processes (two-component regulators, quorum sensing and c-di-gmp signaling). here, we compare and contrast mechanisms and regulation of biofilm fo ... | 2009 | 19231189 |
| vibrio vulnificus: disease and pathogenesis. | 2009 | 19255188 | |
| enhancement of immunity and disease resistance in the white shrimp, litopenaeus vannamei, by the probiotic, bacillus subtilis e20. | effects of bacillus subtilis e20 isolated from fermented soybean on immune parameters and the disease resistance of the white shrimp (litopenaeus vannamei) after 98 days of b. subtilis e20 feeding were evaluated in this study. shrimp fed b. subtilis e20-containing diets at concentrations of 10(6) (e206), 10(7) (e207), and 10(8) (e208)cfu kg(-1), respectively, had significantly increased survival rates of 13.3%, 16.7%, and 20%, compared to the control (fed no probiotic) after being challenged wit ... | 2009 | 19111620 |
| nanobody-aided structure determination of the epsi:epsj pseudopilin heterodimer from vibrio vulnificus. | pseudopilins form the central pseudopilus of the sophisticated bacterial type 2 secretion systems. the crystallization of the epsi:epsj pseudopilin heterodimer from vibrio vulnificus was greatly accelerated by the use of nanobodies, which are the smallest antigen-binding fragments derived from heavy-chain only camelid antibodies. seven anti-epsi:epsj nanobodies were generated and co-crystallization of epsi:epsj nanobody complexes yielded several crystal forms very rapidly. in the structure solve ... | 2009 | 19118632 |
| structure of a polysaccharide from the lipopolysaccharide of vibrio vulnificus cect4602 containing 2-acetamido-2,3,6-trideoxy-3-[(s)- and (r)-3-hydroxybutanoylamino]-l-mannose. | a polysaccharide was isolated by gpc after mild acid treatment of the lipopolysaccharide of vibrio vulnificus cect4602 and found to contain l-rha, d-glcpnac and 2-acetamido-2,3,6-trideoxy-3-(3-hydroxybutanoylamino)-l-mannose (l-rhanac3nhb). glc analysis of the trifluoroacetylated (s)-2-octyl esters derived by full acid hydrolysis of the polysaccharide showed that approximately 80% of the 3-hydroxybutanoic acid has the s configuration and approximately 20% the r configuration. the following struc ... | 2009 | 19128797 |
| regulation of the vibrio vulnificus hupa gene by temperature alteration and cyclic amp receptor protein and evaluation of its role in virulence. | availability of free iron is extremely limited in the mammalian host, and the acquisition of iron in the host is essential for successful infection by pathogenic bacteria. expression of many genes involved in acquiring iron is regulated in response to the level of iron availability, and iron regulation is mediated by fur. in this study, cellular levels of vibrio vulnificus hupa, a heme receptor protein, and the hupa transcript were found to increase in cells grown at 40 degrees c compared to cel ... | 2009 | 19139193 |
| evaluation of genotypic and phenotypic methods to distinguish clinical from environmental vibrio vulnificus strains. | vibrio vulnificus is a heterogeneous bacterial species that comprises virulent and avirulent strains from environmental and clinical sources that have been grouped into three biotypes. to validate the typing methods proposed to distinguish clinical from environmental isolates, we performed phenotypic (api 20e, api 20ne, and biolog tests) and genetic (ribotyping and dna polymorphism at several loci) studies with a large strain collection representing different biotypes, origins, and host ranges. ... | 2009 | 19139234 |
| vibrio vulnificus cytolysin induces apoptosis in huvec, sgc-7901 and smmc-7721 cells via caspase-9/3-dependent pathway. | vibrio vulnificus cytolysin (vvc) is known to be a pore-forming toxin which shows cytotoxicity for mammalian cells in culture and induces apoptosis in endothelial cells. in order to determine whether vvc induces apoptosis in vascular endothelial cells and tumor cells, the cytotoxicity induced by recombinant vvc (rvvc) and its potential mechanism in huvec, sgc-7901 and smmc-7721 cells were investigated. our study demonstrated that rvvc induced the release of intracellular k(+) from all the target ... | 2009 | 19167479 |
| vibrio vulnificus-induced cell death of human mononuclear cells requires ros-dependent activation of p38 and erk 1/2 mapks. | vibrio vulnificus is a gram-negative bacterium that multiplies rapidly in host tissue and causes extensive tissue damage. human peripheral blood mononuclear cells (pbmc) were shown to be readily killed by exposure to live v. vulnificus. v. vulnificus induced production of intracellular reactive oxygen species (ros) and nitric oxide (no) in pbmc. pretreatment of pbmc with diphenyleneiodonium chloride (dpi) abolished ros generation upon exposure to v. vulnificus and decreased the bacterial ability ... | 2009 | 19172484 |
| complete sequence of plasmid pmp1 from the marine environmental vibrio vulnificus and location of its replication origin. | a novel cryptic plasmid, pmp1, from an environmental vibrio vulnificus mp-4 isolated from mai po nature reserve in hong kong, has been characterized. the 7.6-kb plasmid had guanine-cytosine content of 40.03% and encoded four open reading frames (orfs) with >100 amino acids. the predicted protein of orf1 contained 478 amino acids showing 29% identity and 50% similarity over 309 amino acids to the integrase of vibrio cholerae phage vp2. orf2 encoded a putative protein of 596 amino acids, which wer ... | 2009 | 19009320 |
| vibrio vulnificus: an unusual mode of acquisition and novel use of rapid susceptibility testing. | infection with vibrio vulnificus is uncommon in europe but is associated with necrotising wound infections and life-threatening septicaemia. this case is one of infection most likely to have been acquired from a thermal pool in turkey without preceding exposure to seawater or shellfish. the report also describes how early management was optimised using gradient diffusion antibiotic strips to provide rapid susceptibility data. | 2009 | 19028760 |
| expression of the cpda gene, encoding a 3',5'-cyclic amp (camp) phosphodiesterase, is positively regulated by the camp-camp receptor protein complex. | the intracellular level of cyclic 3',5'-amp (camp), a signaling molecule that mediates a variety of cellular processes, is finely modulated by the regulation of its synthesis, excretion, and degradation. in this study, camp phosphodiesterase (cpda), an enzyme that catalyzes the conversion of camp to amp, was characterized in a pathogenic bacterium, vibrio vulnificus. the cpda gene exists in an operon composed of mutt, yqib, cpda, and yqia, the transcription of which was initiated at position -22 ... | 2009 | 19028903 |
| multi-site analysis reveals widespread antibiotic resistance in the marine pathogen vibrio vulnificus. | vibrio vulnificus is a serious opportunistic human pathogen commonly found in subtropical coastal waters, and is the leading cause of seafood-borne mortality in the usa. this taxon does not sustain prolonged presence in clinical or agricultural settings, where it would undergo human-induced selection for antibiotic resistance. therefore, few studies have verified the effectiveness of commonly prescribed antibiotics in v. vulnificus treatment. here we screened 151 coastal isolates and 10 primary ... | 2009 | 18642041 |
| effect of in vitro exposure to vibrio vulnificus on hydroelectrolytic transport and structural changes of sea bream (sparus aurata l.) intestine. | the everted gut sac technique has been used to investigate the effect of vibrio vulnificus on water and electrolyte (na(+), k(+), cl(-), hco(3)(-)) transport on the intestine of sea bream (sparus aurata l.). both the anterior and the posterior intestine were incubated in a medium containing 10(8) v. vulnificus cells ml(-1) at 25 degrees c for 2 h. the presence of v. vulnificus resulted in a significant reduction (p < 0.05) of water absorption in the anterior intestine, while sodium absorption in ... | 2009 | 18825505 |
| sensitive and rapid identification of vibrio vulnificus by loop-mediated isothermal amplification. | vibrio vulnificus is a serious bacterial pathogen for humans and aquatic animals. we developed a rapid, sensitive and specific identification method for v. vulnificus using loop-mediated isothermal amplification (lamp) technique. a set of primers, composed of two outer primers and two inner primers, was designed based on the cytolysin gene sequence of v. vulnificus. the lamp reaction was processed in a heat block at 65 degrees c for 60 min. the amplification products were detected by visual insp ... | 2009 | 18585020 |
| safety of intraaortic balloon counterpulsation in patients with acute myocardial infarction receiving streptokinase intravenously. | 2009 | 2801563 | |
| [the results of an investigation of the various factors influencing the late patency ratio of coronary bypass grafting]. | the purpose of this communication was to find out the factors influencing the late patency ratio of coronary artery bypass grafts of 42 patients in our institution. the following results were obtained: 1. the size of the measured diameters of coronary arteries and the graft flow rates were correlated well to the late patency ratio (p less than 0.05). 2. the regular physical exercise trainings were significantly important to keep patent grafts (p less than 0.01). 3. in blood serum studies, serum ... | 2009 | 2786538 |
| a photographic study of enamel defects among south australian school children. | colour photographs were taken of the anterior teeth of 1757 consenting 8-9 year old primary school children in fluoridated and non-fluoridated areas of south australia. children with a lifelong exposure to fluoridated water were significantly more likely to have blemished permanent incisor teeth than the children who had never resided in a fluoridated area. children who resided in a non-fluoridated area but had taken fluoride supplements at some stage in their first five years had a prevalence o ... | 2009 | 2818303 |
| pilomatrix carcinoma: role played by mr imaging. | we report the magnetic resonance imaging (mri) of a pilomatrix carcinoma. we found a soft tissue tumor of the back entering the spinal canal and compressing the spinal cord and we monitored a good response to radiotherapy and chemotherapy. we have concluded that mri played an important role in determination of the volume, extension and management of this rare malignant tumor. | 2009 | 2747902 |
| changing the school system. | 2009 | 2738019 | |
| proliferating giant pigmented nevus: a report of an unusual tumor occurring in association with a congenital giant pigmented nevus. | a 32-year-old japanese woman with a giant pigmented congenital nevus of the torso presented with a massive pigmented tumor mass of the vulva which grew over an 8-year period. histologically, the tumor was composed of benign appearing nevus-like cells with focal areas of extensive fibrous response. the tumor cells were positive for s-100 protein and with an antihuman melanoma antibody (moab 225, 28s) stain. electron microscopy confirmed the nevomelanocytic nature of the tumor cells and demonstrat ... | 2009 | 2715665 |
| [conventional insulin therapy, infusion pumps, injector syringes. different methods of administration]. | 2009 | 2665113 | |
| [occupational diseases and their reporting]. | 2009 | 2734931 | |
| stereotactic evacuation of hypertensive cerebellar hemorrhage using plasminogen activator. | treatment for hypertensive cerebellar hemorrhage still remains controversial as to whether direct surgical procedure is indicated or not. this is so even after the introduction of ct scan which easily demonstrates the location and size of the hematoma and the presence of hydrocephalus. in this paper, we present our experience of 20 patients with cerebellar hemorrhage treated by stereotactic evacuation using komai's ct-stereotactic apparatus. all the patients had vertigo, cerebellar symptoms, dys ... | 2009 | 2674756 |
| [a different view of sykstus lewkowicz]. | 2009 | 2698489 | |
| [the place of provisional tissues in the histologic classification, used for pedagogical purposes]. | 2009 | 2730350 | |
| new system helps prevent medi-cal claim delays. | 2009 | 2649431 | |
| [the link between cholesterol and atherosclerosis: intervention trials]. | intervention trials aimed at influencing the course of atherosclerosis by reducing blood cholesterol levels of two types: some of them evaluate the clinical consequences of atherosclerosis, while the others study the disease itself by means of arteriography. several trials of the first type have convincingly shown that the incidence of coronary disease decreases after at least 5 years of medicinal treatment. as for direct visualization trials, they seem to indicate that the development of athero ... | 2009 | 2662362 |
| no association between post-transplant erythrocytosis, thromboembolic events, and cyclosporine therapy. | 2009 | 2652691 | |
| the conversion of [35s] ptc-angiotensin i to ptc-angiotensin ii in plasma of normotensive and hypertensive subjects. | 2009 | 4313539 | |
| [relapsing polychondritis. an infrequent cartilage disease]. | report of a case affecting a middle-age woman, 48, involving the e.n.t. area only. the aa comment on the features of the disease and report on the good results with medical treatment (prednisolone, indomethacin and dapsone). | 2009 | 2655492 |
| lead toxicity and iron deficiency in utah migrant children. | we determined the frequency of presumptive iron deficiency and lead toxicity in 198 utah migrant children, ages 9-72 months, during the summer of 1985. there were no confirmed cases of lead toxicity. thirteen per cent of those tested and 30 per cent of the children ages 9-23 months were iron deficient. hematocrit determinations accurately predicted iron deficiency in only 35 per cent of the children confirmed to have this disorder via erythrocyte protoporphyrin screening. | 2009 | 2650572 |
| lead toxicity and iron deficiency in utah migrant children. | we determined the frequency of presumptive iron deficiency and lead toxicity in 198 utah migrant children, ages 9-72 months, during the summer of 1985. there were no confirmed cases of lead toxicity. thirteen per cent of those tested and 30 per cent of the children ages 9-23 months were iron deficient. hematocrit determinations accurately predicted iron deficiency in only 35 per cent of the children confirmed to have this disorder via erythrocyte protoporphyrin screening. | 2009 | 2650572 |
| diagnostic and therapeutic technology assessment (datta). noninvasive electrical stimulation for nonunited bone fracture. | 2009 | 2783614 | |
| mode of processing and hemisphere differences in the judgement of musical stimuli. | the relationship between processing strategy and ear asymmetry was examined in two experiments in which subjects were required to make judgements about monoaurally presented musical chords. in the first experiment, subjects without musical training showed a significant left ear advantage while in those with musical training a significant shift towards a right ear advantage was observed. performance on the embedded figures test (eft) suggested a strong relationship between analytic processing sty ... | 2009 | 2736338 |
| how accurate are faculty evaluations of clinical competence? | to determine the degree and sources of variability faculty evaluations of residents for the american board of internal medicine (abim) clinical evaluation exercise (cex). | 2009 | 2723833 |
| the incidence and significance of seminal vesicle invasion in patients with adenocarcinoma of the prostate. | the incidence and significance of seminal vesicle invasion in patients with adenocarcinoma of the prostate were determined in 139 patients who underwent radical prostatectomy. of the 36 patients who had seminal vesicle invasion, 27 (75%) also had capsular invasion and 13 (43%) had lymph node metastases. disease progression rates were 31% and 58% at 5 and 8 years, respectively. survival rate at 5 years was 90.6% and at 8 years it was 83.7%. of the 103 patients without seminal vesicle invasion, 21 ... | 2009 | 3815316 |
| comparative dna reassociation kinetics of cranes. | 2009 | 2775167 | |
| comparative dna reassociation kinetics of cranes. | 2009 | 2775167 | |
| effects of public day-care: a longitudinal study. | 119 swedish children were followed from their first year of life up to the age of 8. most could be classified according to (a) type of day-care they had experienced during their first 7 years of life and (b) time of first entrance into day-care. at 8 years, the children were tested with aptitude tests and rated by their teachers on school performance and social and personal development. hierarchical regression analyses and mancovas were used in the statistical treatment. time of entrance into da ... | 2009 | 2758882 |
| [ultrasonic diagnosis of tumorous lesions of the liver]. | 2009 | 2698513 | |
| ultrastructural analysis of axosomatic contacts on functionally identified primate spinothalamic tract neurons. | the morphology and frequency of axosomatic contacts on three functionally identified primate spinothalamic tract (stt) cells were analyzed at the electron microscopic level. the stt cells analyzed were wide-dynamic-range neurons responsive to activation of low- and high-threshold cutaneous afferents innervating the foot. the somas were located in the lateral border of lamina v; the dendritic trees were oriented dorsally and were very extensive. numerous spinelike appendages were observed emanati ... | 2009 | 2708581 |
| short-chain fatty acid transport in the intestine of a herbivorous teleost. | short-chain fatty acid (scfa) concentrations of intestinal contents from the herbivorous tilapia, oreochromis mossambicus, were analysed by gas and high-performance liquid chromatography. concentrations of acetate ranging from 3 to 18 mmol l-1 were found along the full length of the intestinal tract, as were trace amounts of propionate. characteristics of [3h]acetate transport by the intestinal brush-border membrane were analysed in isolated brush-border membrane vesicles (bbmv). influx of [3h]a ... | 2009 | 2836545 |