Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| radiation in multidisciplinary treatment of children with malignant ovarian tumors. | thirteen girls with malignant ovarian tumors treated at memorial hospital from august 1971 through october 1975 with extensive surgery, multi-drug chemotherapy (t-2 protocol) and postoperative radiation were reviewed. the plan of radiation was to deliver 3,000 rads to the whole abdomen and boost the para-aortic and pelvic area with 1,500 rads in five to six weeks. in only two patients was the radiation conducted as planned. in others the treatment was either interrupted or discontinued due to co ... | 1998 | 106952 |
| [pancreatic carcinoma on endoscopic retrograde pancreatico-cholangiogram (author's transl)]. | the pancreaticographic appearances of carcinoma of the pancreas have been divided into two types: 1. canalicular carcinoma arising from the duct system. if arising from the main pancreatic duct, it occludes this, or causes displacement or deformity of its branches in the immediate neighbourhood. if arising from branches, it causes cystic ectasia of the minor ducts; these appear fragmented and deformed while there is stenosis and displacement of the main duct. simultaneous origin from the main an ... | 1998 | 124684 |
| genetic and molecular organization of the 5s locus and mutants in d. melanogaster. | the topography of an entire redundant locus was analyzed by both genetic and molecular means. three mutants (min0, min1, min2) allelic to the 5s rrna genetic locus on chromosome 2 of d. melanogaster were isolated. flies exhibit a mutant phenotype when hemizygous for a min allele, but flies having two doses are wild-type. saturation hybridization experiments show that the alleles are gross defieciencies each deleting an equal amount of 5s dna. each of the three mutant min alleles produces a disti ... | 1999 | 103626 |
| primary care approaches to developmental disabilities. | ninety-seven board certified pediatricians who spend at least 75% of their professional working hours involved in the delivery of primary care in new england were interviewed to explore their attitudes and current clinical approaches to developmental disabilities. the majority of the pediatricians relied exclusively on clinical judgment and general observations for assessing developmental problems in their offices. responsibility for preschool screening for potential learning problems and the as ... | 1999 | 91155 |
| otoneurological approach to diagnosis and removal of acoustic neuroma. | 1999 | 91118 | |
| sterilisation issues. | exception is taken to the 2 bases for sterilization of mentally handicapped people as stated by the working group in current medical/ethical problems -- genetic and fitness for parenthood. although parents with certain forms of retardation have a greater genetic propensity to bear mentally retarded offspring, there are considerably more "normal" parents with as high or a greater propensity. 80-90% of mentally handicapped children are born to normal parents. the unfitness for parenthood rationale ... | 1999 | 91007 |
| benzodiazepines and tardive dyskinesia. | 1999 | 90985 | |
| retinol-binding protein in malnutrition. | 1999 | 90984 | |
| [skeletal maturation and its relation to clinical orthodontics]. | 1999 | 96392 | |
| [perception of patients in intensive care units. problems perceived and expectations in relation to nursing care]. | 1999 | 94171 | |
| analysis of the yolk proteins in drosophila melanogaster. translation in a cell free system and peptide analysis. | 1999 | 103753 | |
| [palliative chemotherapy with 5 fu and cpm in cancer of the prostate with bone metastases resistant to oestrogens. a clinical trial (author's transl)]. | the efficacy of an association of cyclophosphamide (cpm) and 5-fluorouracil was studied in 15 patients with prostate cancer not responding to oestrogen therapy, and more particularly its effect on pain due to bone metastases. no objective improvement was noted with this association, but there was a definitite reduction in bone metastases pain in 5 of the patients, with an average remission time of 4 months. half of the patients had nausea and vomiting, but in spite of this digestive intolerance ... | 1999 | 94108 |
| the mesencephalic exterolateral posterior nucleus of the mormyrid fish bryenomyrus niger: efferent connections studied by the hrp method. | 1999 | 91414 | |
| hexacarbon neuropathy. | 1999 | 91030 | |
| [distribution of dental plaque accumulation on permanent tooth surfaces in primary school children]. | 1999 | 91630 | |
| the use of visual feedback to control vocal intensity and nasalization. | a device which provides visual feedback of vocal intensity, nasalization, or a ratio of nasalization to vocal intensity, is described. the device was used in several experiments in which control of vocal intensity was a necessary component. normal and hypernasal speakers were able to control their vocal intensities successfully when using the visual display. the display also was used in an experiment designed to determine how well normal speakers can manipulate their nasalization without visual ... | 1999 | 89126 |
| the relationship between intracellular and extracellular potassium in normal and malnourished subjects as studied in leukocytes. | leukocyte potassium has been measured in normal subjects and in malnourished children with and without edema both on admission and during recovery. in nonedematous malnourished children the potassium content was low (328 mmol/kg dry solids) and took 2-3 weeks of rehabilitation to return to normal (374 mmol/kg dry solids). leukocytes from edematous children had normal intracellular potassium values. however, 5 days on a maintenance diet reduced the intracellular potassium in the children with kwa ... | 1999 | 99717 |
| captopril in a hyponatraemic hypertensive: need for caution in initiating therapy. | 1999 | 85145 | |
| large decompressive craniectomy in management of severe cerebral contusion. a review of 207 cases. | 1999 | 91129 | |
| group systems of serum proteins and erythrocyte enzymes in malignant lymphoma. | four group systems of serum proteins (hp, gc, gm, km) and five group systems of erythrocyte enzymes (ap, pgm1, gpt, ak, esd) were determined in 63 patients with malignant lymphoma. statistical analysis of the distribution of the above mentioned systems in patients and polish population samples did not reveal any significant differences, which points to the lack of any correlation between the disease and the group systems under examination. | 1999 | 85572 |
| plasma arginine vasopressin in diabetic ketoacidosis. | plasma arginine vasopressin (avp) was measured serially in 10 patients during the first 24 hours of treatment of diabetic ketoacidosis. avp was elevated in all cases initially, ranging from 4.0--122.0 pmol/l, the basal level in healthy hydrated subjects being 1.57 +/- 0.59 pmol/l (+/- 1 sd). the levels fell progressively during the course of treatment. while there was no evidence that the massive increases in avp noted in this study were associated with water overload it remains to be determined ... | 1999 | 103767 |
| [treatment of acute diabetic ketoacidosis by administering small insulin doses into the serum]. | 1999 | 99774 | |
| tetralogy of fallot in adults. | 1999 | 91440 | |
| prostaglandin e, pessaries for induction of labour. | vaginal pessaries containing 3 mg of prostaglandin e2 were used to induce labour in 200 patients with variable induction features. prostaglandin-induced labour was augmented where necessary by synthetic oxytocin. there was on failed induction. only 23% of patients with favourable induction features and 53% of patients with unfavourable features needed oxytocin. there were no adverse fetal or maternal effects. the prostaglandin e2 pessary was as effective in inducing labour as 350 microgram extra ... | 1999 | 85163 |
| biochemical changes in the skin of mink with ehlers-danlos syndrome: increased collagen biosynthesis in the dermis of affected mink. | 1999 | 21930 | |
| chido, rodgers and c4. in vivo and in vitro coating of red blood cells, grouping and antibody detection. | c4 sucrose/low ionic strength (lis)-coated red blood cells (rbc) are excellent for the detection of the previously 'nebulous' antibodies, anti-chido and anti-rodgers, as well as for serum/plasma typing of these antigens by an inhibition technique. by enzyme treatment of such cells, it is confirmed that the ch and rg antigens reside on the c4d part of the c4 molecule. freshly taken rbc from normal individuals were examined with a sensitive auto-analyzer technique with anti-chido, anti-c4 and anti ... | 1999 | 91269 |
| [evaluation of blood-liquor barrier by protein gradients in congenital hydrocephalus in infancy]. | 1999 | 88669 | |
| ectopic pregnancy in southern iran: a statistical review of 96 cases. | a review is presented of 96 cases of ectopic pregnacy over a 7-year period. it is noted that in recent years the incidence of this problem has increased. inadequately diagnosed and treated pelvic inflammatory disease has been found to be the main predisposing factor (40%). the rate of extra-uterine gestation was 1:336 deliveries. in this study, previous fertility was high, and primigravidae constituted only 10.4% of the total. the effect of previous pelvic operations and pelvic infection is disc ... | 1999 | 15916 |
| histochemical study of orcein-positive hepatocellular material in paraffin sections of liver biopsy samples in the course of various liver diseases. | a histochemical study was carried out on orcein-positive hepatocellular material in 111 cases, 27 of which were stained positively by orcein. orcein-positive material was very frequently found in protracted viral hepatitis and in chronic active hepatitis, as well as in other liver diseases with or without cholestasis; it was absent in liver cirrhosis. in all the cases considered the orcein-positive material was mainly formed of proteins, rich in amino acids with sulfhydryl and disulphide radical ... | 1999 | 91188 |
| [the mechanism of antigen-antibody reaction at the basement membrane zone in bullous pemphigoid. the question of interaction of complement factors and bivalent cations (author's transl)]. | 1. in a medium poorly cationic indirect immunfluorescence of the basement membrane zone normally observed with preparations of sera from patients with pemphigoid has not be seen. 2. the fluorescence of basement membrane zone takes place if fe2+ ions are added together with the antibasement zone antibodies. evidently fe2+ ions are essential for fixing antibodies. 3. with the method of indirect fluorescence the c3 component of complement could be observed after fixation of pemphigoid antibodies to ... | 1999 | 56158 |
| evidence for an hla-linked resistance gene in buerger's disease. | the incidence of thromboangiitis obliterans in brothers and the high prevalence in some ethnic groups have led us to investigate the histocompatibility hla-a, b and dr antigens of 46 buerger's disease patients. the main result indicates a marked decreased freqeuncy of the b12 antigen: 2.2% vs 28% in controls. our study suggests that buerger's disease is, on the basis of hla antigens, a distinct immunogenetic entity and not a particular form of arteriosclerosis. moreover, this disorder may repres ... | 1999 | 91227 |
| sodium iron nafeedta as an iron fortification compound in central america. absorption studies. | studies were performed in seven children and 98 adults to compare the proportion of iron absorbed when administered as ferric sulfate (fe2(so4)3), nafeedta, hemoglobin (hb), and ferrous ascorbate. studies in children (mostly iron deficient) showed that when the compounds were given with a milk-rice-sugar formula totalling 5 mg fe, iron from hemoglobin was absorbed best, followed by nafeedta and by fe2(so4)3 (mean percent absorption +/-sd = 34.5 +/- 1.5, 8.6 +/- 1.9 and 3.3 +/- 1.5, respectively) ... | 1999 | 96688 |
| secondary mania. | 1999 | 84956 | |
| [role of axoplasmc transport in the pathogenesis of cottony exudates]. | 1999 | 91450 | |
| two effects of phosphodiesterase inhibitors on limulus ventral photoreceptors. | 1999 | 91407 | |
| [treatment of allergic non-seasonal rhinitis with intal]. | 1999 | 91152 | |
| geographical features of estuaries for neritid gastropods including clithon retropictus to preserve thermostable direct hemolysin-producing vibrio parahaemolyticus. | thermostable direct hemolysin-producing strain of vibrio parahaemolyticus was not detected from the alimentary tract of 7 neritid gastropods including clithon retropictus at 9 estuaries of southwest islands in japan in the present study. the strain has been detected from c. retropictus at 2 estuaries facing the sea of japan but not at 2 estuaries facing the seto inland sea and the pacific ocean in western japan in our previous studies. in comparison with geographical features of the estuaries wh ... | 1999 | 10423701 |
| comparison of effects of wasabia japonica and allyl isothiocyanate on the growth of four strains of vibrio parahaemolyticus in lean and fatty tuna meat suspensions. | lean tuna meat suspensions (lean), with a fat content of 0.006%, and fatty tuna meat suspension (fatty), with a fat content of 3.0% were inoculated with four strains of vibrio parahaemolyticus and wasabi (wasabia japonica matsumura) or allyl isothiocyanate (ait) was added before incubation at 37 degrees c. during the incubation, viable vibrio counts were determined on tcbs agar plates. both lean and fatty suspensions were inoculated with v. parahaemolyticus aoto-81, (1.28+/-0.20) x 10(2) cfu/ml, ... | 1999 | 10477067 |
| on the in-vitro antimicrobial activity of oleuropein and hydroxytyrosol. | secoiridoides (oleuropein and derivatives), one of the major classes of polyphenol contained in olives and olive oil, have recently been shown to inhibit or delay the rate of growth of a range of bacteria and microfungi but there are no data in the literature concerning the possible employment of these secoiridoides as antimicrobial agents against pathogenic bacteria in man. in this study five atcc standard bacterial strains (haemophilus influenzae atcc 9006, moraxella catarrhalis atcc 8176, sal ... | 1999 | 10504039 |
| ribotyping of vibrio parahaemolyticus isolates obtained from food poisoning outbreaks in taiwan. | vibrio parahaemolyticus is a prevalent food-borne pathogen in taiwan, japan and other asian countries. this work presents a novel ribotyping method for the molecular epidemiological examination of this pathogen. genomic dna was fragmented by hindiii digestion and hybridized with cdna probe for escherichia coli 16s and 23s rna genes. a total of 121 isolates obtained from outbreaks during 1992 and 1994 in taiwan were characterized by this ribotyping method. four to seventeen restricted fragments w ... | 1999 | 10529103 |
| update on emerging infections from the centers for disease control and prevention. outbreak of vibrio parahaemolyticus infection associated with eating raw oysters and clams harvested from long island sound--connecticut, new jersey, and new york, 1998. | this article is part of a regular series on emerging infections from the centers for disease control and prevention (cdc) and the emergency id net, an emergency department-based and cdc-collaborative surveillance network. important infectious disease public health information with relevance to emergency physicians is reported. the goal of this series is to advance knowledge about communicable diseases in emergency medicine, and foster cooperation between the front line of clinical medicine and p ... | 1999 | 10533020 |
| vibriophage kvp40 and coliphage t4 genomes share a homologous 7-kbp region immediately upstream of the gene encoding the major capsid protein. | vibriophage kvp40, a large tailed dna phage morphologically similar to t-even coliphages, has a major capsid protein (mcp) homologous to the equivalent protein, gp23(*), of coliphage t4. the sequence analysis was extended to a 7-kbp region immediately upstream of the mcp gene encoding the precursor of mcp. the region as a whole was fairly homologous to the corresponding region of the t4 genome and contained 8 orfs homologous to t4 genes 17, 18, 19, 20, 67, 68, 21, and 22 in the same order as in ... | 1999 | 10550672 |
| siderophore production of vibrio parahaemolyticus strains from different sources. | vibrio parahaemolyticus strains isolated from different sources were assayed for their ability to produce a siderophore, vibrioferrin, under iron-limited growth conditions. the mean value +/- standard error of mean (microm vibrioferrin in spent culture supernatant/optical density at 660 nm) was 832.3 +/- 66.9 for clinical isolates (n=44), which was significantly higher (p<0.01) than those for food isolates (461.0 +/- 66.5; n=37) and coastal isolates (378.8 +/- 37.2; n=26). this suggests that gre ... | 1999 | 10553685 |
| [analysis of thermostable direct hemolysin-producing vibrio parahaemolyticus by pulsed-field gel electrophoresis]. | we investigated the source of thermostable direct hemolysin-producing vibrio parahaemolticus infection (positive strains) that causes vibrio parahaemolticus food poisoning. | 1999 | 10565185 |
| vibrio parahemolyticus bacteremia: case report. | vibrio parahemolyticus (v. parahemolyticus) is a halophilic gram-negative bacillus that lives in the ocean. it is the leading cause of infectious diarrhea in taiwan and sometimes produces soft tissue infections, but it is rarely a cause of bacteremia. there have been only 11 cases reported in the literature. most of the cases involved a history of ingestion of seafood or exposure to seawater. in addition, those patients were all immunosuppressed, especially with leukemia and cirrhosis. we report ... | 1999 | 10584427 |
| molecular typing of vibrio parahaemolyticus isolates, obtained from patients involved in food poisoning outbreaks in taiwan, by random amplified polymorphic dna analysis. | vibrio parahaemolyticus is one of the most important food-borne pathogens in taiwan, japan, and other countries with long coastlines. this paper reports on the development of a new random amplified polymorphic dna (rapd) method for the molecular typing of this pathogen. the 10-mer primer 284 (5'-cag gcg cac a-3') was selected to generate polymorphic amplification profiles of the genomic dna at an annealing temperature of 38 degrees c. a total of 308 clinical isolates of v. parahaemolyticus colle ... | 1999 | 10325328 |
| disappearance of glyceraldehyde 3-phosphate dehydrogenase from erythrocyte membrane by hemolysis with thermostable direct hemolysin of vibrio parahaemolyticus or vibrio cholerae el tor hemolysin. | it is believed that the thermostable direct hemolysin (tdh) of vibrio parahaemolyticus and el tor hemolysin (eth) of vibrio cholerae damage erythrocytes and other cells by acting as pore-forming toxins. in this study, we found that a protein band with a molecular weight of 37,000 daltons specifically disappeared from erythrocyte membrane after hemolysis by tdh and eth, but not after hypotonic hemolysis. the 37 kda band was identified as glyceraldehyde 3-phosphate dehydrogenase (g3pd), a glycolyt ... | 1999 | 10338202 |
| characterization of the antiseptic-resistance gene qace delta 1 isolated from clinical and environmental isolates of vibrio parahaemolyticus and vibrio cholerae non-o1. | the nucleotide sequence and mechanism of action were examined on the antiseptic-resistance gene qace delta 1 that had been isolated from pseudomonas aeruginosa, vibrio parahaemolyticus and vibrio cholerae non-o1. the nucleotide sequences of qace delta 1 genes isolated from environmental isolates of v. cholerae non-o1 and v. parahaemolyticus differed by one base from that of the gene from p. aeruginosa. escherichia coli c600 that harbored qace delta 1 genes from several strains of vibrio spp. exh ... | 1999 | 10339831 |
| occurrence, diversity, and pathogenicity of halophilic vibrio spp. and non-o1 vibrio cholerae from estuarine waters along the italian adriatic coast. | the occurrence, diversity, and pathogenicity of vibrio spp. were investigated in two estuaries along the italian adriatic coast. vibrio alginolyticus was the predominant species, followed by vibrio parahaemolyticus, non-o1 vibrio cholerae, and vibrio vulnificus. by using a biochemical fingerprinting method, all isolates were grouped into nine phenotypes with similarity levels of 75 to 97.5%. the production of toxins capable of causing cytoskeleton-dependent changes was detected in a large number ... | 1999 | 10347072 |
| response of pathogenic vibrio species to high hydrostatic pressure. | vibrio parahaemolyticus atcc 17802, vibrio vulnificus atcc 27562, vibrio cholerae o:1 atcc 14035, vibrio cholerae non-o:1 atcc 14547, vibrio hollisae atcc 33564, and vibrio mimicus atcc 33653 were treated with 200 to 300 mpa for 5 to 15 min at 25 degrees c. high hydrostatic pressure inactivated all strains of pathogenic vibrio without triggering a viable but nonculturable (vbnc) state; however, cells already existing in a vbnc state appeared to possess greater pressure resistance. | 1999 | 10347079 |
| comparison of the heme iron utilization systems of pathogenic vibrios. | vibrio alginolyticus, vibrio fluvialis, and vibrio parahaemolyticus utilized heme and hemoglobin as iron sources and contained chromosomal dna similar to several vibrio cholerae heme iron utilization genes. a v. parahaemolyticus gene that performed the function of v. cholerae huta was isolated. a portion of the tonb1 locus of v. parahaemolyticus was sequenced and found to encode proteins similar in amino acid sequence to v. cholerae hutw, tonb1, and exbb1. a recombinant plasmid containing the v. ... | 1999 | 10348876 |
| sequence of the v. parahaemolyticus gene for cytoplasmic n, n'-diacetylchitobiase and homology with related enzymes. | the nucleotide sequence of the gene encoding the cytoplasmic n, n'-diacetylchitobiase [ec 3.2.1.14] from vibrio parahaemolyticus (atcc #27969) has been determined. the deduced peptide sequence of this unusual beta-hexosaminidase surprisingly shows minimum evolutionary relationship to two other reported n, n'-diacetylchitobiases from vibrios, except in highly conserved regions which are also homologous to lysosomal beta-hexosaminidases from eukaryotes including humans. in contrast, the two other ... | 1999 | 10348911 |
| [food poisonings caused by halophilic vibrios in the city of vladivostok: their epidemiological characterization]. | 1999 | 10356733 | |
| clonal diversity among recently emerged strains of vibrio parahaemolyticus o3:k6 associated with pandemic spread. | the genomes of the o3:k6 strains of vibrio parahaemolyticus which abruptly emerged in calcutta, india, in february 1996 and which demonstrated an unusual potential to spread and an enhanced propensity to cause infections were examined by different molecular techniques to determine clonality. no restriction fragment length polymorphism (rflp) in the gene encoding the thermostable direct hemolysin was observed among the o3:k6 isolates of v. parahaemolyticus. clonal diversity among the o3:k6 strain ... | 1999 | 10364615 |
| detection of total and hemolysin-producing vibrio parahaemolyticus in shellfish using multiplex pcr amplification of tl, tdh and trh. | vibrio parahaemolyticus is an important human pathogen which can cause gastroenteritis when consumed in raw or partially-cooked seafood. a multiplex pcr amplification-based detection of total and virulent strains of v. parahaemolyticus was developed by targeting thermolabile hemolysin encoded by tl, thermostable direct hemolysin encoded by tdh, and thermostable direct hemolysin-related trh genes. following optimization using oligonucleotide primers targeting tl, tdh and trh genes, the multiplex ... | 1999 | 10379807 |
| a filamentous phage of vibrio parahaemolyticus o3:k6 isolated in laos. | a filamentous phage, 'lvpf5,' of vibrio parahaemolyticus o3:k6 strain lvp5 was isolated and characterized. the host range was not restricted to serotype o3:k6, but 7 of 99 v. parahaemolyticus strains with a variety of serotypes were susceptible to the phage. the phage was inactivated by heating at 80 c for 10 min and by treating with chloroform. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the phage exhibited a 3.8 kda protein. the amino-terminal amino acid sequence of the coat p ... | 1999 | 10385206 |
| factors influencing in vitro killing of bacteria by hemocytes of the eastern oyster (crassostrea virginica). | a tetrazolium dye reduction assay was used to study factors governing the killing of bacteria by oyster hemocytes. in vitro tests were performed on bacterial strains by using hemocytes from oysters collected from the same location in winter and summer. vibrio parahaemolyticus strains, altered in motility or colonial morphology (opaque and translucent), and listeria monocytogenes mutants lacking catalase, superoxide dismutase, hemolysin, and phospholipase activities were examined in winter and su ... | 1999 | 10388697 |
| comparison of molecular methods for typing vibrio parahaemolyticus. | an outbreak of vibrio parahaemolyticus gastroenteritis on canada's west coast in 1997 emphasized the need to develop molecular methods for differentiation and typing of these organisms. isolates were analyzed by enterobacterial repetitive intergenic consensus sequence (eric) pcr, detection of restriction fragment length polymorphisms (rflp) in rrna genes (ribotyping), pulsed-field gel electrophoresis (pfge), and rflp analysis of the genetic locus encoding the polar flagellum (fla locus rflp anal ... | 1999 | 10405387 |
| bactericidal activities of essential oils of basil and sage against a range of bacteria and the effect of these essential oils on vibrio parahaemolyticus. | basil and sage essential oils were examined for bactericidal activity against a range of gram-positive and gram-negative bacteria by viable count determinations. generally, gram-positive bacteria showed higher resistance to basil and sage essential oils than gram-negative bacteria. vibrio species showed a high sensitivity to both essential oils. stationary growth phase cells of selected bacteria showed higher resistance to these essential oils than exponential growth phase cells. basil-resistant ... | 1999 | 10652788 |
| fulminant necrotising fasciitis caused by vibrio parahaemolyticus. | we report a patient with septicaemia and fulminant necrotising fasciitis caused by vibrio parahaemolyticus. this organism is strongly associated with seawater exposure and seafood ingestion. the patient recovered due to expedient management, prompt recognition of the organism, appropriate antimicrobial cover and surgical debridement. the lesson to be learned is that this organism should be clinically suspected and recognised from its typical history of injury and fulminant clinical progress as a ... | 1999 | 10628251 |
| cloning and characterization of manganese superoxide dismutase gene from vibrio parahaemolyticus and application to preliminary identification of vibrio strains. | the soda gene coding for manganese superoxide dismutase (mn-sod) from the marine microorganism vibrio parahaemolyticus was cloned, sequenced, and overexpressed in escherichia coli by use of the pet20b (+) expression vector. the full-length gene consisted of a 588-bp open reading frame and encoded a polypeptide of 196 amino acid residues, with a calculated molecular mass of 21,713 da. the recombinant enzyme was efficiently purified from crude e. coli cell lysate by metal ion affinity chromatograp ... | 1999 | 10690650 |
| incidence of highly genetically diversified vibrio parahaemolyticus in seafood imported from asian countries. | vibrio parahaemolyticus is an important pathogen of humans and aquacultured animals, especially in asian countries. in this study, we examined 686 samples of seafood imported from hong kong, indonesia, thailand and vietnam for v. parahaemolyticus. v. parahaemolyticus was recovered from 315 (45.9%) samples. the incidence of v. parahaemolyticus in products from hong kong and thailand was markedly higher than the incidence in products from indonesia and vietnam. the incidence rates in shrimp, crab, ... | 1999 | 10733249 |
| emergence and prevalence of a novel vibrio parahaemolyticus o3:k6 clone in japan. | 1999 | 10738364 | |
| a mechanism of resistance to hydrogen peroxide in vibrio rumoiensis s-1. | a possible mechanism of resistance to hydrogen peroxide (h2o2) in vibrio rumoiensis, isolated from the h2o2-rich drain pool of a fish processing plant, was examined. when v. rumoiensis cells were inoculated into medium containing either 5 mm or no h2o2, they grew in similar manners. a spontaneous mutant strain, s-4, derived from v. rumoiensis and lacking catalase activity did not grow at all in the presence of 5 mm h2o2. these results suggest that catalase is inevitably involved in the resistanc ... | 1999 | 9872762 |
| outbreak of vibrio parahaemolyticus infection associated with eating raw oysters and clams harvested from long island sound--connecticut, new jersey, and new york, 1998. | during july-september 1998, an outbreak of vibrio parahaemolyticus infections associated with consumption of oysters and clams harvested from long island sound occurred among residents of connecticut, new jersey, and new york. this is the first reported outbreak of v. parahaemolyticus linked to consumption of shellfish harvested from new york waters. this report summarizes the investigation of this outbreak. | 1999 | 9935142 |
| vibrio parahaemolyticus thermostable direct hemolysin modulates cytoskeletal organization and calcium homeostasis in intestinal cultured cells. | vibrio parahaemolyticus is a marine bacterium known to be the leading cause of seafood gastroenteritis worldwide. a 46-kda homodimer protein secreted by this microorganism, the thermostable direct hemolysin (tdh), is considered a major virulence factor involved in bacterial pathogenesis since a high percentage of strains of clinical origin are positive for tdh production. tdh is a pore-forming toxin, and its most extensively studied effect is the ability to cause hemolysis of erythrocytes from d ... | 1999 | 10024554 |
| from the centers for disease control and prevention. outbreak of vibrio parahaemolyticus infection associated with eating raw oysters and clams harvested from long island sound--connecticut, new jersey, and new york, 1998. | 1999 | 10029109 | |
| evaluation of alkaline phosphatase- and digoxigenin-labelled probes for detection of the thermolabile hemolysin (tlh) gene of vibrio parahaemolyticus. | the biochemical identification and enumeration of vibrio parahaemolyticus as described in the fda bacteriological analytical manual is expensive and labour-intensive. to reduce the time and effort necessary to verify the identity of v. parahaemolyticus, the use of a thermolabile haemolysin (tlh) gene probe is proposed. an alkaline phosphatase (ap)-labelled probe was evaluated for specificity against 26 strains of v. parahaemolyticus, 88 strains of other vibrio species and 10 strains of non-vibri ... | 1999 | 10030035 |
| randomly amplified polymorphic dna analysis of clinical and environmental isolates of vibrio vulnificus and other vibrio species. | vibrio vulnificus is an estuarine bacterium that is capable of causing a rapidly fatal infection in humans. a randomly amplified polymorphic dna (rapd) pcr protocol was developed for use in detecting v. vulnificus, as well as other members of the genus vibrio. the resulting rapd profiles were analyzed by using rflpscan software. this rapd method clearly differentiated between members of the genus vibrio and between isolates of v. vulnificus. each v. vulnificus strain produced a unique band patte ... | 1999 | 10049874 |
| isolation and characterization of vibrio parahaemolyticus causing infection in iberian toothcarp aphanius iberus. | high mortality among laboratory cultured iberian toothcarp aphanius iberus occurred in february 1997 in valencia (spain). the main signs of the disease were external haemorrhage and tail rot. bacteria isolated from internal organs of infected fish were biochemically homogeneous and identified as vibrio parahaemolyticus. the bacteria were haemolytic against erythrocytes from eel anguilla anguilla, amberjack seriola dumerili, toothcarp a. iberus and humans, and were kanagawa-phenomenon-negative. i ... | 1999 | 10073316 |
| identification of vibrio parahaemolyticus strains at the species level by pcr targeted to the toxr gene. | the dna colony hybridization test with the polynucleotide probe for vibrio parahaemolyticus toxr gene was performed. all 373 strains of v. parahaemolyticus gave positive results, and the strains belonging to four other vibrio species including vibrio alginolyticus gave weakly positive results, suggesting that toxr sequence variation may reflect the phylogenetic relationships of vibrio species. we then established a toxr-targeted pcr protocol for the specific detection of v. parahaemolyticus. | 1999 | 10074546 |
| [vibrio parahaemolyticus enteritis]. | 1999 | 10088453 | |
| physical and genetic map of the genome of vibrio parahaemolyticus: presence of two chromosomes in vibrio species. | we constructed a physical map of the genomic dna (5.1 mb) for vibrio parahaemolyticus strain aq4673 by combining 17 adjacent noti fragments. this map shows two circular replicons of 3.2 and 1.9 mb. pulsed-field gel electrophoresis (pfge) of undigested genomic dna revealed two bands of corresponding sizes. analysis both by noti digestion and by southern blot of the two isolated bands confirmed the existence of two replicons. the presence of genes for 16s rrna on both the replicons indicates that ... | 1999 | 10200969 |
| expression and characterization of the prtv gene encoding a collagenase from vibrio parahaemolyticus in escherichia coli. | the prtv gene, encoding a collagenase of vibrio parahaemolyticus, was expressed in escherichia coli and purified by affinity chromatography. the transformant e. coli bl21(de3)(pprt2) secreted the recombinant prtv, and the highest enzyme activity was detected in the culture supernatant after 5 h iptg induction. the molecular mass of purified prtv was 62 kda as determined by gel filtration, which was similar to that obtained by sds-page (64 kda). this suggested that prtv was a monomer protein havi ... | 1999 | 10206692 |
| [bacteriological studies of traveller's diarrhoea (6). analysis of enteropathogenic bacteria at kansai airport quarantine station from september 4th, 1994 through december 1996]. | during the period of investigation from sept. 4, 1994 to dec, 1996, a total of 11,446,534 overseas travellers were quarantined at kansai airport quarantine station, and 22,187 voluntarily reported of episodes suffering from diarrhoea. bacteriological examination of the stools a total of 9,299 individuals' was performed, and the following results were obtained. 1) various enteropathogenic bacteria were isolated from 33.3% of the stools examined. bacterial species isolated were as follows: plesiom ... | 1999 | 10213987 |
| [comparison of epidemiological markers for vibrio parahaemolyticus isolated from food poisoning]. | vibrio parahaemolyticus food poisoning, is the most prevalent among bacterial food poisoning in japan. study of epidemiologic markers is important in an attempt to trace the source of contamination. the purpose of this study was to compare seven different typing methods (serotyping, plasmid profile, antibiogram, phage susceptibility. tdh production, tdh and trh gene and pulsed-field gel electrophoresis [pfge]) for v. parahaemolyticus. outbreaks of v. parahaemolyticus food poisoning which occurre ... | 1999 | 10213996 |
| macromolecular antimicrobial glycoprotein, achacin, expressed in a methylotrophic yeast pichia pastoris. | a cdna encoding achacin, an antimicrobial glycoprotein from the body surface mucus of giant african snail achacina fulica férussac, was expressed in a methylotrophic yeast, pichia pastoris, and recombinant achacin (rach) was secreted in yeast minimal medium in a polyglycosylated form with 80 kda. carbohydrate analysis revealed that the glycosylated moiety of rach was composed of 50 mol mannose and 2 mol n-acetylglucosamine residues. antimicrobial activity using escherichia coli and staphylococcu ... | 1999 | 10217406 |
| sequence analysis of the gyra and parc homologues of a wild-type strain of vibrio parahaemolyticus and its fluoroquinolone-resistant mutants. | vibrio parahaemolyticus causes seafood-borne gastroenteritis in humans. it is particularly important in japan, where raw seafood is frequently consumed. fluoroquinolone is one of the current drugs of choice for treating patients infected by v. parahaemolyticus because resistant strains are rarely found. to study a possible fluoroquinolone resistance mechanism in this organism, nucleotide sequences that are homologous to known gyra and parc genes have been cloned from v. parahaemolyticus aq3815 a ... | 1999 | 10223929 |
| mutations conferring resistance to phenamil and amiloride, inhibitors of sodium-driven motility of vibrio parahaemolyticus. | the bacterial flagellum is powered by a rotary motor capable of turning the helical flagellar propeller at very high speeds. energy to drive rotation is derived from the transmembrane electrochemical potential of specific ions. ions passing through a channel component are thought to generate the force to power rotation. two kinds of motors, dependent on different coupling ions, have been described: proton-driven and sodium-driven motors. there are four known genes encoding components of the sodi ... | 1999 | 10318954 |
| the multiple identities of vibrio parahaemolyticus. | vibrio parahaemolyticus is a ubiquitous marine bacterium and human pathogen. the organism possesses multiple cell types appropriate for life under different circumstances. the swimmer cell, with a single polar flagellum, is adapted to life in liquid environments. the polar flagellum is powered by the sodium motive force and can propel the bacterium at fast speeds. the swarmer cell, propelled by many proton-powered lateral flagella, can move through highly viscous environments, colonize surfaces, ... | 1999 | 10941784 |
| some emerging food and water borne pathogens. | emerging pathogens are those infective organisms whose incidence has recently increased or is likely to increase during the next two decades due to changes in demography, food habits, food technology, commerce, water sources and environmental factors. some important emerging food and water borne bacterial pathogens include listeria monocytogenes, campylobacter jejuni, yersinia enterocolitica, salmonella enteritidis, escherichia coli o157: h7, vibrio cholerae biotype e1 tor serotype 0139, vibrio ... | 1999 | 10810592 |
| vibrio parahaemolyticus, japan, 1996-1998. | 1999 | 10887775 | |
| acid adaptation induces cross-protection against some environmental stresses in vibrio parahaemolyticus. | the relationship of acid adaptation to the resistance of other environmental stresses was examined in vibrio parahaemolyticus. acid-adapted cells were found to have increased resistance to various stresses, including heat, crystal violet, bile, and deoxy cholic acid. however, heat-adapted cells showed no increased resistance against acid stress. adaptation required protein synthesis, since treatment with chloramphenicol during adaptation to ph 5.3 prevented the development of acid resistance. ac ... | 1999 | 12501372 |
| [antibiotic resistance and plasmid profiles of vibrio isolates from cultured sparus sarba]. | a total of 51 potential pathogenic vibrios were isolated from moribund silver seabream sparus sarba, which were collected from local fish farms of hong kong. all the isolates were classified and identified as 7 species by the api 20 e system and the scheme of alsina & blanch. these species were vibrio alginolyticus (24 strains), vibrio vulnificus (12 strains), vibrio parahaemolyticus(7 strains), vibrio logei(4 strains), vibrio pelagius ii(2 strains), vibrio fluvialis (1 strains) and vibrio medit ... | 1999 | 12555529 |
| [effect of irradiation on the shelf-life of aquatic products]. | the effect of irradiation at doses of 0-10 kgy on the shelf-life of aquatic products was determined. the results showed that irradiation could not prolong the shelf-life of aquatic products kept at 25 degrees c. while the aquatic products were kept at refrigerator (4 degrees c), the irradiation could prolong the shelf-life and effectively reduce the total aerobic plate count. irradiation could effectively reduce the number of coliform to less than 300/kg and eliminate shigella shigae, salmonella ... | 2000 | 12725095 |
| [acute gastroenteritis caused by urease positive vibrio parahaemolyticus in an immunocompromised patient]. | 2000 | 10905019 | |
| characterization of vibrio parahaemolyticus isolates obtained from foodborne illness outbreaks during 1992 through 1995 in taiwan. | vibrio parahaemolyticus is an important foodborne pathogen in taiwan and many other asian countries. a total of 371 isolates of v. parahaemolyticus collected from patients involved in foodborne illness outbreaks in taiwan from 1992 to 1995 were characterized. these isolates had typical biochemical characteristics and only 4% were urease positive. the most frequently isolated serovars were o5:k15 (18.5%), o4:k8 (16.2%), o3:k29 (12.5%), o1:k56 (8.3%), o2:k3 (6.5%), and o4:k12 (6.0%). most of the i ... | 2000 | 10914657 |
| analysis of gyrb and toxr gene sequences of vibrio hollisae and development of gyrb- and toxr-targeted pcr methods for isolation of v. hollisae from the environment and its identification. | isolation of vibrio hollisae strains, particularly from the environment, is rare. this may be due, in part, to the difficulty encountered when using conventional biochemical tests to identify the microorganism. in this study, we evaluated whether two particular genes may be useful for the identification of v. hollisae. the two genes are presumed to be conserved among the bacterial species (gyrb) or among the species of the genus vibrio (toxr). a portion of the gyrb sequence of v. hollisae was cl ... | 2000 | 10919814 |
| food poisoning outbreak from contaminated fish-balls. | on february 9th, 1998, a food poisoning outbreak occurred at a boarding school for underprivileged students. an unmatched case-control study was done. an environmental survey, laboratory study of rectal swab culture, fish-balls, water and the cooking utensils were also performed. there were 132 suspect cases, of which the attack rate in teachers was 9.8 per cent (4/41), 16.7 per cent (1/6) in the food handlers and 15.7 per cent (127/810) in the students. the median incubation period was 18 hours ... | 2000 | 11215857 |
| characterization of vibrio parahaemolyticus manganese-resistant mutants in reference to the function of the ferric uptake regulatory protein. | in many bacteria, the ferric uptake regulatory protein (fur) has a central role in the negative regulation of genes affected by iron limitation. in this study, vibrio parahaemolyticus strains carrying mutations in the fur gene encoding fur were isolated by the manganese selection method to assess the function of fur in connection with alternations in the coordinate expression of the siderophore vibrioferrin (vf) and iron-repressible outer membrane proteins (iromps). ten out of 25 manganese-resis ... | 2000 | 11220684 |
| enterotoxicity and cytotoxicity of vibrio parahaemolyticus thermostable direct hemolysin in in vitro systems. | vibrio parahaemolyticus is a marine bacterium known to be a common cause of seafood gastroenteritis worldwide. the thermostable direct hemolysin (tdh) has been proposed to be a major virulence factor of v. parahaemolyticus. tdh causes intestinal fluid secretion as well as cytotoxicity in a variety of cell types. in this study, we investigated the interplay between the hemolysin's enterotoxic and cytotoxic effects by using both human and rat cell monolayers. as revealed by microspectrofluorimetry ... | 2000 | 10816461 |
| vibrio parahaemolyticus infections in the united states, 1973-1998. | vibrio parahaemolyticus infections are associated with consumption of raw or undercooked shellfish, contaminated food, and exposure of wounds to warm seawater. foodborne outbreaks and sporadic infections from vibrio species in 4 gulf coast states are reported routinely to the centers for disease control and prevention (cdc). between 1988 and 1997, 345 sporadic v. parahaemolyticus infections were reported: 59% were gastroenteritis, 34% were wound infections, 5% were septicemia, and 2% were from o ... | 2000 | 10823766 |
| isolation of a pandemic o3:k6 clone of a vibrio parahaemolyticus strain from environmental and clinical sources in thailand. | application of an immunomagnetic enrichment method selective for vibrio parahaemolyticus serovar k6 allowed isolation of a strain belonging to the pandemic o3:k6 clone of v. parahaemolyticus from fresh shellfish not implicated in a clinical case in southern thailand. arbitrarily primed pcr profiles of this strain, clinical o3:k6 strains isolated from sporadic diarrhea cases in the same area, and a standard pandemic o3:k6 strain were indistinguishable. | 2000 | 10831459 |
| chemical and serological properties of lipopolysaccharides from vibrio parahaemolyticus o-untypeable strains isolated from patients. | chemical and serological studies have been carried out on the o-antigenic lipopolysaccharides (lps) of six strains, u-6443, w-90144, x-3972, ad-7999, 90a-6611 and kx-v212, of vibrio parahaemolyticus isolated from patients. the o-serotypes of these strains have not been identified because they were not agglutinated by any diagnostic antisera against known o-serotype strains. a compositional sugar analysis of their lps revealed that out of the six o-untypeable (out) strains, u-6443, w-90144 and ad ... | 2000 | 10832965 |
| a filamentous phage associated with recent pandemic vibrio parahaemolyticus o3:k6 strains. | a specific serotype, o3:k6, of vibrio parahaemolyticus has recently been causing epidemics of gastroenteritis in southeast asia, japan, and north america. to examine whether the new o3:k6 strains possess characteristics that may exacerbate outbreaks, we compared v. parahaemolyticus o3:k6 strains with non-o3:k6 strains using strains isolated from individuals with traveler's diarrhea at kansai airport quarantine station, osaka, japan. all 24 o3:k6 strains possessed a common plasmid, po3k6 (dna siz ... | 2000 | 10834969 |
| molecular characterization of vibrio parahaemolyticus vsglt: a model for sodium-coupled sugar cotransporters. | the na(+)/galactose cotransporter (vsglt) of vibrio parahaemolyticus, tagged with c-terminal hexahistidine, has been purified to apparent homogeneity by ni(2+) affinity chromatography and gel filtration. resequencing the vsglt gene identified an important correction: the n terminus constitutes an additional 13 functionally essential residues. the mass of his-tagged vsglt expressed under its native promoter, as determined by electrospray ionization-mass spectrometry (esi-ms), verifies these 13 re ... | 2000 | 10835424 |
| analysis of the polar flagellar gene system of vibrio parahaemolyticus. | vibrio parahaemolyticus has dual flagellar systems adapted for locomotion under different circumstances. a single, sheathed polar flagellum propels the swimmer cell in liquid environments. numerous unsheathed lateral flagella move the swarmer cell over surfaces. the polar flagellum is produced continuously, whereas the synthesis of lateral flagella is induced under conditions that impede the function of the polar flagellum, e.g., in viscous environments or on surfaces. thus, the organism possess ... | 2000 | 10850984 |
| antibacterial activity of a chitooligosaccharide mixture prepared by cellulase digestion of shrimp chitosan and its application to milk preservation. | the antibacterial activity of a chitooligosaccharide mixture prepared by digestion of shrimp chitosan with cellulase at 50 degrees c for 14 h was evaluated. sugars with 1 to 8 degrees of polymer (dp) were found in this chitooligosaccharide mixture, and the weight percentage of sugars with dp > or = 6 was 44.3%. minimal lethal concentrations of this mixture against aeromonas hydrophila, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella typhimurium, shigella dysenteriae, ... | 2000 | 10852568 |
| characterization of the vibrio parahaemolyticus na+/glucose cotransporter. a bacterial member of the sodium/glucose transporter (sglt) family. | the vibrio parahaemolyticus sodium/glucose transporter (vsglt) is a bacterial member of the sglt gene family. wild-type and mutant vsglt proteins were expressed in escherichia coli, and transport activity was measured in intact cells and plasma membrane vesicles. two cysteine-less vsglt proteins exhibited sugar transport rates comparable with that of the wild-type protein. six residues in two regions of vsglt known to be of functional importance in sglt1 were replaced individually with cysteine ... | 2000 | 10852908 |
| the use of colony hybridization in the isolation of thermostable direct hemolysin-producing vibrio parahaemolyticus from foods implicated in an incidence of food poisoning. | 2000 | 10871921 |