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isolation and characterization of a second exe operon required for extracellular protein secretion in aeromonas hydrophila.strain c5.84 is a tn5-751 insertion mutant of aeromonas hydrophila which is unable to secrete extracellular proteins, instead accumulating them in the periplasm (b. jiang and s.p. howard, j. bacteriol. 173:1241-1249, 1991). a 3.5-kb bglii fragment which complements this mutation was isolated from the chromosome of the parent strain. analysis of this fragment revealed an operon-like structure with two complete genes, exea and exeb, a functional promoter 5' to the exea gene, and a 13-bp inverted r ...19947961440
oral challenge with aeromonas in protein-malnourished mice.the lack of an animal model for aeromonas-associated diarrhoea has hindered progress toward understanding the pathogenesis of this potentially important enteric infection. protein-malnourished mice were challenged orally with aeromonas strains to determine if diminished levels of resistance would allow the induction of a diarrhoeal response. the 15 aeromonas spp. faecal isolates used for challenge included 7 a. caviae, 4 a. hydrophila, 1 a. sobria bv. sobria, and 1 a. sobria bv. veronii from pat ...19947963338
modulation of the bacterial clearance activity of haemocytes from the freshwater mollusc, lymnaea stagnalis, by the avian schistosome, trichobilharzia ocellata.the ability of haemocytes, from the haemolymph of the gastropod mollusc lymnaea stagnalis, to recognize and eliminate the bacterium aeromonas salmonicida was shown using an in vitro bacterial clearance assay. the assay employs a dye which is reduced by a. salmonicida in direct proportion to the number of viable bacteria resulting in a colour change which can be determined spectrophotometrically. addition of cytochalasin b resulted in a marked decrease in bacterial clearance, implicating both int ...19947970887
bacterial populations in the groundwater on the us-mexico border in el paso county, texas.in el paso county, texas, 73 domestic wells were sampled using a variety of selective media to determine the extent of bacterial contamination of the groundwater on the us side of the united states-mexico border. thirteen wells were contaminated by fecal coliforms, whereas other wells contained a variety of bacterial genera including some potential pathogens that normally would not be detected by standard methods of water testing.19947973920
antigen-induced release of macrophage activating factor from rainbow trout oncorhynchus mykiss leucocytes.the production of macrophage-activating factor (maf) by rainbow trout, oncorhynchus mykiss, head kidney leucocytes at varying times post-immunisation, with the fish bacterial pathogen, aeromonas salmonicida, was investigated and correlated with head kidney lymphocyte proliferation and serum antibody production. maf production was preceded by lymphocyte proliferation and both responses were highest using whole bacterial cells as the in vitro stimulant. maf production and antibody production incre ...19947975191
immunoregulation in fish. i: intramolecular-induced suppression of antibody responses to haptenated protein antigens studied in atlantic salmon (salmo salar l).we report here evidence for intramolecular-induced suppression of the in vivo antibody response in fish, using a panel of t-dependent hapten-carrier antigens. atlantic salmon were immunized intraperitoneally with protein antigens (limulus polyphemus hemocyanin, chicken gamma globulin, and aeromonas salmonicida a-layer protein) given in their native form or haptenated with either 4-hydroxy-3-iodo-5-nitrophenyl-acetic acid (nip), 2,4,6-trinitrophenyl-acetic acid (tnp), or fluorescein-5-iso-thiocya ...19947521853
an endo-acting proline-specific oligopeptidase from treponema denticola atcc 35405: evidence of hydrolysis of human bioactive peptides.an endo-acting proline-specific oligopeptidase (prolyl oligopeptidase [popase], ec 3.4.21.26) was purified to homogeneity from the triton x-100 extracts of cells of treponema denticola atcc 35405 (a human oral spirochete) by a procedure that comprised five successive fast protein liquid chromatography steps. the popase is a cell-associated 75- to 77-kda protein with an isoelectric point of ca. 6.5. the enzyme hydrolyzed (optimum ph 6.5) the pro-pna bond in carbobenzoxy-gly-pro-p-nitroanilide (z- ...19947523301
a histological study of the pathological effect of lipopolysaccharide-free glycerophospholipid: cholesterol acyl transferase (gcat) on atlantic salmon (salmo salar l.). 19947698454
pcr-based preparation of 23s rrna-targeted group-specific polynucleotide probes.dna coding for a variable region within domain iii of bacterial 23s rrna was used as the target for group-specific polynucleotide hybridization probes. the corresponding rdna was amplified in vitro by the pcr technique in combination with a pair of primers specific for flanking conserved target sites. the amplified fragments were cloned or used directly as probes. rna probes were generated by in vitro transcription of cloned or amplified rdna. the probes were labeled by incorporating modified nu ...19947524442
variable expression of o-antigen and the role of lipopolysaccharide as an adhesin in aeromonas sobria.on initial isolation of aeromonas sobria 3767 from a diarrhoeal stool specimen, two colony types were obtained: opaque (3767o) and translucent (3767t). strain 3767o consistently produced lipopolysaccharide (lps) core and o-antigen side chain, detectable by sds-page and by western blotting with an o-antigen-specific monoclonal antibody. strain 3767t produced lps core but the amount of o-antigen was dependent on factors including growth medium and bacterial growth phase. strain 3767t exhibited sig ...19947525403
aeromonas salmonicida resistance to complement-mediated killing.the resistance of aeromonas salmonicida to complement-mediated killing was investigated by using different strains and their isogenic mutants that had been previously characterized for their surface components. we found that the classical complement pathway is involved in serum killing of susceptible a. salmonicida strains, while the alternative complement pathway seems not to be involved. all of the a. salmonicida strains are able to activate complement, but the smooth strains (with or without ...19947525485
the c-terminal peptide produced upon proteolytic activation of the cytolytic toxin aerolysin is not involved in channel formation.the channel-forming toxin aerolysin is secreted by aeromonas hydrophila as a protoxin that can be activated by nicking with endoproteinase lys-c after lys-427 near the c terminus of the protein. the fate of the 43-amino acid peptide distal to the activation site was investigated. a cysteine was introduced into the c-terminal region by replacing ile-445, and another replaced gly-202, which is on the proximal side of the activation site. in a double mutant, the two new cysteines were close enough ...19947527031
detection of aeromonas salmonicida, causal agent of furunculosis in salmonid fish, from the tank effluent of hatchery-reared atlantic salmon smolts.the fish pathogen, aeromonas salmonicida, could be detected only by bacteriological culture from the kidney of dead or moribund fish in one tank in a hatchery rearing atlantic salmon (salmo salar l.) smolts. however, by using a dna probe specific for this species, allied to a pcr assay, the pathogen could be detected in water, feces and effluent samples taken from this fish tank. also, the presence of the pathogen was found in effluent samples from two fish tanks containing apparently healthy fi ...19947527205
homology between the seed cytolysin enterolobin and bacterial aerolysins.enterolobin, a 55-kda cytolytic, inflammatory, and insecticidal protein isolated from seeds of the brazilian tree enterolobium contortisiliquum (leguminosae-mimosoideae) has been further purified and partially sequenced by using both manual and automated methods. a computational search of enterolobin partial amino acid sequence against the pir database revealed possible sequence similarities with aerolysins, cytolytic proteins from aeromonas species. an alignment of enterolobin partial sequence ...19947710657
development of antibiotic-resistant strains for the enumeration of foodborne pathogenic bacteria in stored foods.strains of aeromonas spp., salmonella enteritidis phage type 4, salmonella typhimurium, verotoxigenic escherichia coli o157:h7 (vtec) and yersinia enterocolitica resistant to streptomycin, nalidixic acid and a combination of both antibiotics were selected. when compared with the parent strains, most of the antibiotic-resistant strains had slightly slower growth rates at their optimum incubation temperature but the difference was reduced progressively when the temperature was lowered. some antibi ...19947703007
genetic variation in the humoral immune response against vibrio salmonicida and in antibody titre against vibrio anguillarum and total igm in atlantic salmon (salmo salar).total igm level and antibody titre to vibrio anguillarum o-antigen after bath-vaccination, and specific antibody response to v. salmonicida o-antigen at three different samplings were analysed in family material of atlantic salmon (salmo salar), consisting of 791 fish belonging to 34 maternal full-sib groups within 12 paternal half-sib groups. the fish were immunized twice, and blood samples collected three times. after the third blood sampling, the fish were challenged with v. anguillarum. medi ...19947725631
virulence factors in clinical and food isolates of aeromonas species.virulence factors were compared in 15 aeromonas spp. isolated from faeces of patients with aeromonas-associated gastroenteritis and in 81 strains isolated from food. strains from food did not show differences in the distribution of virulence factors when compared with strains isolated from faeces. however, 88.8% of aeromonas strains isolated from food were capable of producing possible virulence factors. characterization of 28 autoagglutinating (aa+) aeromonas spp. indicated that the human strai ...19947729770
relationship between resistance of salmonids to furunculosis and recovery of aeromonas salmonicida from external mucus.fish were sampled at the ed weed state fish hatchery (south hero, vermont, usa) in september 1992. aeromonas salmonicida was common, with concentrations as high as 10(5) to 10(7) colony-forming units per gram of mucus, and readily recovered from most mucus samples obtained from furunculosis-sensitive populations of brook trout (salvelinus fontinalis), lake trout (salvelinus namaycush), and atlantic salmon (salmo salar). the pathogen was the predominant microorganism and accounted for greater tha ...19947760494
studies on the bacterial flora of fish which are potential pathogens for human. isolation of various potential human pathogenic organisms from different parts of fish and their significance in initiating human diseases.freshwater fish of different categories, collected from market of dhaka city of bangladesh, were studied for the bacterial flora. potential human pathogens were isolated from about eighty-five percent of the fish studied. organisms isolated were aeromonas spp., (69.2%), vibrio spp., (42.3%), plesiomonas shigelloides (35.9%) and escherichia coli (16.7%). the high association of potential human pathogens among the freshwater fish suggest that if fish are handled improperly or if consumed undercook ...19947748144
studies on the bacterial flora of fish which are potential pathogens for human. virulence factors of potential human pathogen isolated.tests for various virulence factors, such as production of haemolysin on sheep blood agar plate, cytotoxin on hela cell line and enterotoxin in gm-1 elisa and suckling mouse assay model, were done among the various strains of aeromonas spp., vibrio spp., plesiomonas shigelloides and esch. coli isolated from fresh water fish samples. invasive properties of the isolates were also seen by using sereny test. haemolysin production was observed in 85.7% of aeromonas, all (100%) of vibrios, 13.3% of es ...19947748153
[relationship between bacteria decomposing organic substances and water pollution in river water].in order to clarify the relationship between water pollution and bacteria decomposing organic substances, 48 samples of river water were collected at twelve stations of the chikuma-sai river system. the bacteria decomposing organic substances were enumerated and identified by gm plates, which contained fewer organic substances than agar plates. sixteen biological-physicochemical examinations of the river water were also performed. the results were as follows: temperature and dissolved oxygen in ...19947807705
cloning and expression of a cloxacillin-hydrolyzing enzyme and a cephalosporinase from aeromonas sobria aer 14m in escherichia coli: requirement for an e. coli chromosomal mutation for efficient expression of the class d enzyme.two beta-lactamase genes, asba1 and asbb1, encoding asba1 and asbb1, respectively, have been cloned from aeromonas sobria aer 14m into escherichia coli. asba1 was expressed at low but detectable levels in all e. coli laboratory cloning strains tested. asbb1 was expressed well in the e. coli cloning strain dh5 alpha. however, no enzyme activity could be detected from the same clone when placed in e. coli mc1061. ampicillin-resistant mutants of e. coli mc1061 were obtained that expressed high leve ...19947811022
in vitro activity of fk-037, a novel parenteral cephalosporin, against bacterial isolates from neutropenic cancer patients.the in vitro activity of fk-037, a novel parenteral cephalosporin, was compared to that of ceftazidime, aztreonam and piperacillin (agents often used in empiric regimens in cancer patients) against recent bacterial isolates from patients with cancer. fk-037 was either equal to or 2 to 16-fold more active than the comparative agents against members of the enterobacteriaceae. it was also active against acinetobacter spp., aeromonas spp., pseudomonas aeruginosa, and other pseudomonas spp. xanthomon ...19947813504
dermatologic manifestations of infections in neutropenic patients.skin lesions may serve as the site of origin for disseminated infection in neutropenic patients or result as a secondary consequence of disseminated infection from another source. it is important to recognize the characteristic skin lesions associated with infections, such as those caused by clostridium spp, candida spp, and pseudomonas aeruginosa, so that appropriate therapy is initiated promptly. unfortunately, therapy for some of these infections, especially those caused by fungi, is unsatisf ...19947814839
identification of vibrio vulnificus by cellular fatty acid composition using the hewlett-packard 5898a microbial identification system: collaborative study.a gas chromatographic method using a capillary column for rapid identification of vibrio vulnificus was examined in a collaborative study. identifications were performed by analysis of cellular fatty acid profiles which were automatically searched against reference profiles stored in a computer-generated library. each of the 13 collaborators was sent 15 unknown isolates, which included 10 v. vulnificus isolates and 5 negative control isolates. each collaborator was furnished with a computer-gene ...19947819758
rapid identification of aeromonas species using 16s rdna targeted oligonucleotide primers: a molecular approach based on screening of environmental isolates.published 16s rdna sequencing data for aeromonas species were analysed and the validity of signature sequences derived from our investigations of these sequences was examined by sequencing the corresponding 16s rdna regions of 67 environmental isolates from sewage effluents and receiving waters around sydney and one clinical isolate, all previously classified as aeromonas species. species-specific probes for aer. hydrophila and aer. veronii were designed and tested in pcr assays and clearly disc ...19947822232
[in vitro antibacterial activity of fleroxacin (flrx) against clinical isolates from bacterial enteritis].antibacterial activity of fleroxacin (flrx), a new quinolone antimicrobial, against 36 strains of shigella app., 14 strains of salmonella spp., 11 strains of escherichia coli, 9 strains of vibrio spp. (including 2 strains of v. cholerae o1), 14 strains of campylobacter jejuni/coli, 3 strains of aeromonas spp. and 1 strain of plesiomonas shigelloides isolated from infectious enteritis patients in this study was determined. its activity was compared with that of ciprofloxacin (cpfx), norfloxacin ( ...19947829909
[pili and colonization factors of vibrionaceae]. 19947830312
enteropathogens associated with acute diarrhoeal diseases.five types of escherichia coli are responsible for as much as 25% of all diarrheal diseases in developing countries. they tend to be transmitted via contaminated foods, particularly weaning foods, and water. they include enterotoxigenic, enteropathogenic, enteroadherent, enteroinvasive, and enterohemorrhagic e. coli. shigella species are responsible for 10-15% of acute diarrheas in children less than 5 years old and the most common etiologic agents of childhood dysentery. shigellosis is common i ...19947835992
aeromonas, yersinia, and miscellaneous bacterial enteropathogens. 19947838604
gyra mutations in quinolone-resistant isolates of the fish pathogen aeromonas salmonicida.gyra mutations in quinolone-resistant isolates of aeromonas salmonicida have been detected by using pcr to amplify the quinolone resistance-determining region of gyra and subsequent cloning and sequencing of pcr products. comparison of nucleotide and derived amino acid sequences of pcr products from quinolone-susceptible and -resistant bacteria revealed a serine 83-to-isoleucine substitution in the gyrase a protein of resistant isolates. one of the resistant isolates differed from the other by a ...19947840589
presence of aeromonas hydrophila in slaughtered animals.the fate of aeromonas hydrophila after a bacteriaemia in four healthy pigs intravenously inoculated with 10(9) and 10(15) cells of the bacterium was investigated. in two pigs slaughtered 30 min after inoculation the bacteria were found in blood (8 x 10(2)/ml and 4.2 x 10(2)/ml), meat and fatty tissues (1-10/g), but not in liver, spleen and superficial inguinal lymph nodes. a. hydrophila was not found in blood, meat, fatty tissue, liver, spleen, kidneys and superficial inguinal lymph nodes of oth ...19947848784
comparative in vitro activity of fk-037, a new cephalosporin antibiotic.the in vitro activity of fk-037, a novel parenteral oxime-type cephalosporin, was compared with that of cefepime, cefpirome, ceftazidime, imipenem, and gentamicin against a total of 668 recent clinical isolates. minimum inhibitory concentrations were determined by a standard agar dilution procedure, and all isolates were tested at two inocula (10(4) and 10(6) colony forming units). fk-037 inhibited 90% of isolates of escherichia coli, klebsiella species, proteus mirabilis, p. vulgaris, morganell ...19947867295
septicaemia caused by aeromonas hydrophilia in a neonate. 19947868498
enterotoxigenicity, hemolytic activity and antibiotic resistance of aeromonas spp. isolated from freshwater prawn marketed in dhaka, bangladesh.aeromonas spp. were isolated from gills, swimmerets, eggs, stomachs and ventral muscles of freshwater prawns (macrobrachium malcolmsonii) available in the local fish market of dhaka, bangladesh. the density of aeromonas spp. on these different body parts of the prawn samples ranged from 1.1 +/- 0.2 x 10(4) to 1.5 +/- 0.16 x 10(7) cfu per gram. the viable counts of aeromonads, fecal coliforms (fc) and escherichia coli gradually increased in prawn samples when stored at 4 c. at -20 c, the viable c ...19947869955
detection of shigellae, enteroinvasive and enterotoxigenic escherichia coli using the polymerase chain reaction (pcr) in patients returning from tropical countries.we have used the polymerase chain reaction (pcr) to detect shigellae, eiec and etec in stool specimens of diarrhoeic patients returning from tropical countries. as compared to culture (7.1% positive specimens), which recognizes only shigella strains, pcr performed on bacterial growth from directly inoculated macconkey agar plates and directed against virulence-associated genes present in both shigella and eiec was positive in 19.8% of the samples. we assumed that these additional positive result ...19947870070
incidence of motile aeromonas spp. in foods.a total of 80 food samples were purchased from local retail consumer shops and examined for the presence of motile aeromonas spp. of the food categories tested, poultry had the highest incidence, with 100% positive. this was followed by lamb samples, with 60% positive. raw milk and cheese samples had very low incidence (20%). no motile aeromonas spp. were found in pre-prepared salads. shellfish, fish, pork and beef samples had incidences of 40%. most of the strains isolated were aeromonas hydrop ...19947873101
an example of the stages in the development of a predictive mathematical model for microbial growth: the effects of nacl, ph and temperature on the growth of aeromonas hydrophila.the stages involved in developing a predictive model are illustrated using data describing the effects of temperature (3-20 degrees c), nacl concentration (0.5-4.5% w/v) and ph (4.6-7.0) on the aerobic growth of aeromonas hydrophila (cocktail of 6 strains). optical density measurements using micro-titre plates were used as an initial screen, to determine the appropriate sampling times for viable counts to be made and to determine the approximate boundaries for growth. growth curves were generate ...19947873337
[ecological and food safety considerations about products of vegetable origin].media have paid much attention in recent years to emerging microbiological problems in foods of plant origin. the potential for contamination of fruits and vegetables is high because of the wide variety of conditions to which produce is exposed during growth, harvest, processing and distribution. these considerations acquire great significance in the current scenario of the new processing techniques that offer attributes of convenience and fresh-likeness in response to changes in consumption pat ...19948984963
[clinical bacteriology and empiric therapy for hospital-acquired pneumonia in the elderly at a national leprosarium].hospital-acquired pneumonia is one of the most important fatal respiratory diseases in the elderly. prompt and precise empiric therapy is essential for recovery. fourteen isolates from twelve elderly lepromatous leprosy patients (9 men, 3 women, mean age of 75.8 years) with hospital-acquired pneumonia were studied. subsequently, empiric therapy with gentamicin and beta-lactams for nosocomial pneumonia in the elderly was examined. fourteen types of bacteria isolated from expectorated sputum speci ...19947799554
structure of the aeromonas toxin proaerolysin in its water-soluble and membrane-channel states.aerolysin is chiefly responsible for the pathogenicity of aeromonas hydrophila, a bacterium associated with diarrhoeal diseases and deep wound infections. like many other microbial toxins, the protein changes in a multistep process from a completely water-soluble form to produce a transmembrane channel that destroys sensitive cells by breaking their permeability barriers. here we describe the structure of proaerolysin determined by x-ray crystallography at 2.8 a resolution. the protoxin (m(r) 52 ...19947510043
assessing glycosidic linkage flexibility: conformational analysis of the repeating trisaccharide unit of aeromonas salmonicida.a detailed conformational analysis was performed for the synthetic branched trisaccharide beta-d-mannac-(1-->4)-[alpha-d-glc-(1-->3)]-l-rha 1 which represents the repeating unit of the o-antigenic polysaccharide of aeromonas salmonicida. the study was based on 26 experimental noe curves from 1d transient noe experiments, employing gaussian-shaped inversion pulses at 600 mhz. eight of the noe curves were interglycosidic and thus useful for an analysis of glycosidic linkage orientations. metropoli ...19947510557
the cytolytic toxin aerolysin: from the soluble form to the transmembrane channel.aerolysin is a cytolytic toxin which forms channels in the plasma membranes of eucaryotic cells. the protein is secreted by aeromonas hydrophila as an inactive protoxin. its stability and water solubility are conferred by its ability to dimerize. maturation of the protein occurs through proteolytic removal of a c-terminal peptide outside the secreting cell. although the aerolysin which is so produced is still a dimer, it then has the ability to oligomerize. the oligomer is the active form of the ...19947512760
o-serogrouping and surface components of aeromonas hydrophila and aeromonas jandaei pathogenic for eels.the relationship between virulence, o-serogroup, and some cell-surface features (self-pelleting [sp] and precipitation after boiling [pab], profile of lipopolysaccharides [lpss] and outer membrane proteins [omps]) was investigated in strains of the pathogenic species aeromonas hydrophila and a. jandaei isolated from eels. virulent strains of a. hydrophila reacted mostly with o:19 antiserum, and those of a. jandaei reacted with o:4, o:11, o:15 and o:29 antisera (guinée and jansen system). regardi ...19947514147
[a simple and rapid confirmation method of the bacterial contamination using polymerase chain reaction].we studied the method for detection of bacterial contamination. polymerase chain reaction (pcr) was used to amplify the 888 base pair of 16s ribosomal rna (rrna) gene fragment of various strains of bacterial species. the supernatant of bacterial suspension after treatment for 10 min at 100 degrees c was used for a template dna. a total of 151 strains of 16 genus of gram-negative rod and of one genus of gram-positive cocci were confirmed and were divided into five categories by comparing digestio ...19947517986
carbohydrate-reactive, pore-forming outer membrane proteins of aeromonas hydrophila.two outer membrane proteins of aeromonas hydrophila a6, isolated in a one-step affinity chromatography process based on carbohydrate reactivity, were found to be pore-forming molecules in artificial planar bilayer membranes. these carbohydrate-reactive outer membrane proteins (cromps; m(r)s, 40,000 and 43,000) were subjected to amino acid analysis. the amino acid profiles for these two outer membrane proteins were almost identical. a partial protein sequence of a 14-amino-acid fragment of the 40 ...19947520425
phylogenetic analysis and assessment of the genera vibrio, photobacterium, aeromonas, and plesiomonas deduced from small-subunit rrna sequences.we sequenced nearly complete small-subunit rrnas of 54 reference strains belonging to the genera vibrio, photobacterium, aeromonas, and plesiomonas. we then performed a phylogenetic analysis by comparing the sequences which we obtained with all other known sequences for bacteria belonging to the gamma subgroup of the proteobacteria (thus providing a data base consisting of 70 sequences for the genera investigated), using methods such as neighbor joining, maximum likelihood, and maximum parsimony ...19947520733
serum antibody responses of divers to waterborne pathogens.to assess the significance of exposure of divers to waterborne pathogens, specific immunoglobulin g serum antibody responses to pseudomonas and aeromonas isolates recovered from dive sites from the respiratory tracts of nine experienced divers and seven diving trainees working in the chesapeake bay area over a 6- to 18-month period were measured. a significant increase in the frequency of isolation of these organisms from respiratory surfaces both groups of divers after each dive was noted, with ...19947496942
picture story. from protoxin to pore. 19947544681
[effectiveness of modified atmospheres against psychrotrophic pathogenic microorganisms in proteinaceous food].modified atmosphere packaging (map) of proteinaceous raw foods (meat, poultry and fish) extends their shelf-lives. it is well established that modified atmospheres (ma) inhibit the psychotropic aerobic gram-negative bacteria, the main spoilage microflora of proteinaceous raw foods stored under refrigeration. several researchers have warned about the possible growth of food poisoning microorganisms on them. considering the minimal growth temperatures of pathogens, this review only deals with aero ...19957546447
lactic acid inhibition of the growth of spoilage bacteria and cold tolerant pathogens on pork.the antibacterial effects of a 3% solution of lactic acid at 55 degrees c were assessed, by examining aerobic bacterial growth on artificially-inoculated pork fat and lean tissue. discs of fat or lean tissues, each of 10 cm2 surface area, were aseptically excised from pork longissimus dorsi muscle and inoculated with the cold tolerant pathogens listeria monocytogenes 4b scott a no. 3, yersinia enterocolitica 0:4,32 or aeromonas hydrophila atcc 7966, or with the wild type spoilage bacteria pseudo ...19957547145
buffering capacity and h+ membrane conductance of gram-negative bacteria.buffering capacity and membrane h+ conductance were examined in seven gram-negative species: aquaspirillum serpens, pseudomonas aeruginosa, alcaligenes faecalis, escherichia coli, salmonella typhimurium, proteus mirabilis and aeromonas hydrophila. all strains of enterobacteriaceae studied here showed a decrease in both parameters as the external ph increased, over the ph range studied. the other four species presented an increase in buffering capacity and membrane conductance to protons as the e ...19957557289
immunoregulatory activities of eicosanoids in the rainbow trout (oncorhynchus mykiss).eicosanoids such as prostaglandins (pg), leukotrienes (lt) and lipoxins (lx) have been shown to be potent immunoregulatory molecules in mammals. to determine if they have similar roles in 'lower' animals, rainbow trout (oncorhynchus mykiss) were immunized with either sheep erythrocytes or aeromonas salmonicida in the presence or absence of the stable analogue of pge2, 16,16-dimethyl-pge2, and the number of plaque-forming cells (pfc) or specific antibody levels determined. the higher dose of 16,1 ...19957558126
motile aeromonas as agent of infections of the foot.motile aeromonas infections of the foot are caused mostly by post-traumatic incidence, occurring mostly during summer months. serious complications such as osteomyelitis and amputation can result if the infections go untreated or are inadequately treated. the role of each species of motile aeromonas in pathogenesis and response to antimicrobial agents is not well understood because of taxonomic uncertainty. as a group, motile aeromonas respond well to aminoglycosides, second-generation and third ...19957562462
[many problems in the diagnosis of colitis. 2 case reports]. 19957564651
electron microscopic examination of factors influencing the expression of filamentous surface structures on clinical and environmental isolates of aeromonas veronii biotype sobria.strains of aeromonas veronii biotype sobria isolated from clinical and environmental sources were examined for their expression of surface structures under a variety of culture conditions. when grown on solid media at 37 degrees c, more than 95% of bacteria from the majority of strains isolated from human diarrheal feces and chicken carcasses were non-piliated or expressed only a few pili of long, flexible morphology per cell. strains isolated from water or other foods were much more likely to e ...19957565173
risk of diarrheal disease in ecuadorian day-care centers.to determine the risk for diarrheal disease (dd) in day-care centers (dcc) for children residing in a poor urban slum area of quito, ecuador, compared with that for children from the same environment but cared for in their own residential home (rh), a prospective age-, sex- and locale-controlled study of dd was conducted, including 115 children in dcc and 115 in rh, ages 12 to 42 months. the overall incidence of dd was 46/1000 child weeks. diarrhea was more common in dcc than in rh (relative ris ...19957567291
[occurrence of parasites, bacteria, viruses and fungi in fish that are pathogenic to men and fish].parasitological examinations comprised above 20,000 fish which were searched for parasitic nematoda of anisakidae. it was evidenced that herring were infected with anisakid larvae in 8%, cod and flatfish in 4% and the eelpouts in as many as in 52%. the species that prevails in these fish were: anisakis simplex, contracaecum osculatum c, hysterothylacium auctum and pseudoterranova decipiens, respectively. in direction of the bacteria pathogenic to man 765 fish were examined; in 38% there were fou ...19957571631
interactions of biapenem with active-site serine and metallo-beta-lactamases.biapenem, formerly ljc 10,627 or l-627, a carbapenem antibiotic, was studied in its interactions with 12 beta-lactamases belonging to the four molecular classes proposed by r. p. ambler (philos. trans. r. soc. lond. biol. sci. 289:321-331, 1980). kinetic parameters were determined. biapenem was readily inactivated by metallo-beta-lactamases but behaved as a transient inhibitor of the active-site serine enzymes tested, although with different acylation efficiency values. class a and class d beta- ...19957574520
microflora and tetrodotoxin-producing bacteria in a gastropod, niotha clathrata.shellfish (niotha clathrata) were collected in both july and november from three locations in taiwan (pingtung, kaohsiung and chiai prefecture) and assayed for anatomical distribution of tetrodotoxin (ttx) and aerobic heterotrophic bacteria. pingtung specimens showed higher toxicity than those from kaohsiung and chiai, and did not show much seasonal variation. at each site, the total aerobic bacterial counts in november samples were higher than in july. the predominant genera were vibrio, pseudo ...19957590540
the interaction of trimethoprim and quinolones against gram-negative fish pathogens.the effect of combination of trimethoprim with other non-sulphonamide antibacterial agents, in particular oxolinic acid and nalidixic acid, was evaluated against gram-negative fish pathogens. the species included aeromonas salmonicida, yersinia ruckeri, some vibrio spp. and escherichia coli as a reference. the extent of synergy found by other workers with these substances against human gram-negative bacteria was not apparent here. some positive interaction between trimethoprim and oxolinic acid ...19957592108
rapd analysis of aeromonas salmonicida and aeromonas hydrophila.the randomly amplified polymorphic dna (rapd) technique was used to analyse the genetic differentiation of 13 strains of aeromonas salmonicida subsp. salmonicida, and seven strains of aer. hydrophila. reproducible profiles of genomic dna fingerprints were generated by polymerase chain reaction (pcr) using a single randomly designed primer. the rapd profiles of all the non-motile aeromonads, aer. salmonicida subsp. salmonicida were identical. however, profiles of the motile aeromonads, aer. hydro ...19957592114
[in vitro antibacterial activity of balofloxacin (blfx) against isolates from patients with bacterial enteritis].in vitro antibacterial activity of balofloxacin (blfx), a newly developed fluoroquinoline, was compared with that of norfloxacin (nflx), ofloxacin (oflx) and ciplofloxacin (cpfx). bacterial strains used in this experiment were freshly isolated from patients with infectious enteritis just before blfx therapy. the isolates were 43 strains of vibrio cholerae o1, 1 strain of campylobacter sp., 4 strains of aeromonas spp., 3 strains of plesiomonas shigelloides, 1 strain of vibrio mimicus and 1 strain ...19957594800
a specific puld homolog is required for the secretion of paracrystalline surface array subunits in aeromonas hydrophila.aeromonas hydrophila is an important pathogen of fish, and its high-virulence strains display a two-dimensional paracrystalline layer (s-layer) on their outermost surfaces. the nucleotide sequence of a 4.1-kb region located 700 bp upstream of the a. hydrophila tf7 s-layer protein gene (ahsa) has been determined. a sequence analysis of the region revealed the presence of three complete open reading frames ending in a gene encoding a 79.8-kda polypeptide that shows high homology to the puld family ...19957608063
cloning and primary structure of the chia gene from aeromonas caviae.the chia gene from aeromonas caviae encodes an extracellular chitinase, 865 amino acids long, that shows a high degree of similarity to chitinase a of serratia marcescens. expression in escherichia coli yielded an enzymatically active protein from which a leader sequence was removed, presumably during transport of the enzyme across the cell membrane.19957608101
pseudomonas aeruginosa cytotoxin: the asp197-gly-asp-tyr-his-tyr-his-tyr202 containing loop is critical for plasma membrane binding.the cytotoxin from pseudomonas aeruginosa is a pore-forming toxin that binds specifically to water channel-related molecules of the erythrocyte membrane. here, we have defined a domain, asp197-gly-asp-tyr-his-tyr202 of the cytotoxin, to be essential for receptor binding. cytotoxin point mutants from the recombinant gene carrying substitutions in the domain were characterized in terms of inhibiting the binding of radioiodinated natural cytotoxin to rat erythrocyte and producing cytotoxic effects ...19957609787
aeromonas hydrophila myonecrosis accompanying mucormycosis five years after bone marrow transplantation.a 36-year-old man, five years after bone marrow transplantation for aplastic anemia, was admitted with myonecrosis of the forearm after he had immersed his hand in sewage water several days prior to his admission. blood cultures and specimens taken from the necrotic tissue of the arm all grew aeromonas hydrophila. following extension of the infection, the patient underwent amputation of the arm but ultimately died of cerebral mucormycosis. the epidemiology of aeromonas infections is discussed an ...19957614968
yeast beta-glucan stimulates respiratory burst activity of atlantic salmon (salmo salar l.) macrophages.previous studies have shown that head kidney macrophages isolated from glucan injected rainbow trout (oncorhychus mykiss) and atlantic salmon (salmo salar l.) have increased ability to kill aeromonas salmonicida. the present work was aimed at investigating the in vitro effects of glucan on the respiratory burst and bactericidal potential of atlantic salmon head kidney macrophages. salmon macrophages were incubated for 1-7 days with various concentrations of yeast beta-glucan (macrogard) and test ...19957615137
aeromonas bacteremia: review of 59 episodes.fifty-nine episodes of bacteremia due to aeromonas species occurred within a 5-year period in one medical center in southern taiwan. underlying diseases in the 58 patients included hepatic cirrhosis (36%) and cancer (24%). patients with aeromonas bacteremia more often had underlying hepatic cirrhosis than did those with bacteremia due to other gram-negative bacilli. males (67%) outnumbered females. the cases appeared to cluster in the summer and fall months. thirty-two percent were polymicrobial ...19957620014
a conserved aeromonas salmonicida porin provides protective immunity to rainbow trout.a protein with an apparent m(r) of 28,000 was isolated from outer membrane preparations of aeromonas salmonicida a440. the protein was tested for the ability to form pores, using a planar lipid bilayer model membrane system. the protein appeared to be a monomer with a single-channel conductance in 1.0 m kcl of 1.96 ns and a cation/anion permeability ratio of 2.91 +/- 0.68. these data show that the porin channel is comparable in size to ompc and ompf of escherichia coli and is relatively nonselec ...19957622241
aeromonas infections and their treatment.with advances in the identification and molecular taxonomy of aeromonas spp., these organisms, which are widely distributed in the environment, are increasingly being recognised as human pathogens. clinical infections include gastroenteritis, skin and soft tissue infections and bacteraemia. antibiotic resistance poses a potential problem in the antimicrobial therapy of infections cased by aeromonas spp. while most strains are susceptible to chloramphenicol, ciprofloxacin, co-trimoxazole and the ...19957628980
conserved amino acid residues in the primary structure of ribosomal protein s20 from selected gram-negative bacteria.partial dna sequences encoding ribosomal protein s20 have been determined for a number of gram-negative bacteria including three species of aeromonas, klebsiella pneumoniae, proteus mirabilis, and salmonella typhimurium and compared to the known sequence from escherichia coli. the derived amino acid sequence is well conserved, particularly in the c-terminal one-third of s20. in contrast, there is much less conservation of the nucleotide sequence or potential secondary structure in the correspond ...19957640306
bacterial succession within a biofilm in water supply lines of dental air-water syringes.biofilms have been implicated as reservoirs for bacterial contamination of water delivered by dental air-water syringes. a 6-month study was done of bacterial colonization and biofilm formation in plastic water supply lines connected to dental air-water syringes. changes in biofilm flora were observed by both scanning electron microscopy and bacteriologic culture. by day 7, many rod- and spiral-shaped bacteria had colonized the ridged surface of the luminal wall of the tubing, as revealed by sca ...19957641146
distribution of aeromonas phenospecies and genospecies among strains isolated from water, foods or from human clinical samples.a total of 332 aeromonas spp. originating from drinking water (n = 75), fresh water (n = 57), chicken and ground beef (107), human faecal samples in association with travelling (n = 49), human faecal samples not associated with travelling (n = 38), and six strains from human blood cultures were studied by phenotypic methods and by using analysis of ribopatterns as a molecular method for the identification of the 13 known hybridization groups (hgs). also included were the reference strains of eac ...19957641837
surface phenotypic characteristics and virulence of spanish isolates of aeromonas salmonicida after passage through fish.eleven strains of aeromonas salmonicida were passaged twice by intraperitoneal injection through rainbow trout and reisolated from the kidney of moribund fish. the surface characteristics and virulence of the strains changed following passage through fish. none of the in vitro tests used could effectively predict the in vivo virulence.19957646039
evaluation of the suitability of bis-(1,3-dibutylbarbituric acid) trimethine oxonol, (diba-c4(3)-), for the flow cytometric assessment of bacterial viability.the usefulness of oxonol (bis-(1,3-dibutylbarbituric acid)trimethine oxonol) as a generally applicable indicator of bacterial viability was investigated using untreated and killed cultures of a variety of bacterial genera. killing methods involved either heat or bactericidal antibiotics. for all strains tested, the fluorescent dye showed significantly more intense staining of killed than untreated cells. the sensitivity of aeromonas salmonicida to gentamicin was assessed using oxonol. although t ...19957649437
a survey of enteropathogens among united states military personnel during operation bright star '94, in cairo, egypt.acute gastroenteritis is a potential cause of substantial morbidity in u.s. military personnel during deployment. this study was conducted to evaluate enteric pathogens associated with diarrhea in a u.s. military population on deployment in cairo, egypt, during november 1993. enteric pathogens found to be associated with cases of diarrhea included: enterotoxigenic escherichia coli (etec), 27% (22% heat-stable [st], 3% heat-labile [lt], and 2% st/lt producers); campylobacter spp., 3%; and salmone ...19957659237
media for aeromonas spp., plesiomonas shigelloides and pseudomonas spp. from food and environment.several media are proposed particularly for the detection of aeromonas spp. but also for plesiomonas shigelloides and pseudomonas spp. some are for general purposes and others specifically for the examination of clinical, environmental or food samples. all media are selective, due to antibiotics, bile salts, dyes and other selective agents, as well as differential, primarily based on the ability of the microorganisms to ferment/not ferment carbohydrates. as with all selective media, the recovery ...19957662518
phenotypic and molecular characteristics of aeromonas salmonicida subsp. salmonicida isolated in southern and northern finland.aeromonas salmonicida subsp. salmonicida causes outbreaks of furunculosis in salmonid fish. furunculosis was first detected in finland in 1986 on fish farms located on the finnish coast of the bothnian bay. molecular methods, sds-page, ribotyping, randomly amplified polymorphic dna (rapd) and plasmid profile analysis as well as phenotypic characteristics (biochemical characteristics, maximum growth temperature, pigment and elastase production) were used both for typing the strains and to study t ...19957665387
cell-surface charge and cell-surface hydrophobicity of collagen-binding aeromonas and vibrio strains.partitioning in aqueous polymer two-phase systems of polyethylene glycol and dextran was used to detect and compare cell-surface charge and cell-surface hydrophobicity of aeromonas hydrophila, a. caviae, a. sobria, vibrio cholerae, and v. anguillarum strains. these strains have cell-surface components that bound either native or thermally denatured type i collagen (i.e., a mixture of the alpha1+alpha2 chains) and gelatin immobilized on latex beads. our goals were: (1) to compare the possible rel ...19957668930
acute gastroenteritis in three community-based nursing homes.acute gastroenteritis is a cause of considerable morbidity and mortality in the elderly population. a prospective assessment of acute diarrhea in three community-based long-stay homes is described.19957671026
aeromonads in acute diarrhoea and asymptomatic infections in nigerian children.stool samples of 616 asymptomatic and 296 diarrhoeic school children were compared for the recovery rate of aeromonas spp. on ampicillin (10 micrograms/ml) sheep blood agar. culture filtrates of isolates were tested for haemolysin production with 1% freshly washed rabbit erythrocytes. stools of 9 (3.0%) diarrhoeic children yielded five strains of a. hydrophila and four of a. veronii (two each of biotypes sobria and veronii), compared to 12 (1.9%) (p > 0.01) asymptomatic children who harbored sev ...19957672071
aeromonas septicaemia in hong kong species distribution and associated disease.during a 5-year study of 2211 patients with clinically significant positive blood cultures in hong kong. aeromonas spp. were isolated in 40 cases. among 26 episodes in which a single species was isolated. 17 (65.4%) were identified as aeromonas hydrophila. 8 (30.8%) as a. sobria and one (3.8%) as a. caviae. there were 14 episodes with a mixture of species. of these, nine (64.3%) were identified as a. hydrophila, two (14.3%) as a. sobria, and two (14.3%) as a. caviae. one (7.1%) was an infection ...19957673749
beta-lytic endopeptidases. 19957674960
incidence of pathogenic microorganisms in clinical specimens from hospitals in south-western nigeria.the pattern and frequency of bacterial isolates cultured from 687 clinical specimens from four university teaching hospitals in south western nigeria were determined. a total of 707 bacterial isolates were cultured from 18 different clinical sources giving an average of 1.03 bacteria per sample identified in this collection. of these, gram negative rods constituted 78.1% and the rest were staphylococci. the most common organism isolated in urine samples was e. coli (32.1%) whilst s. aureus (28.9 ...19957498026
non-enteritic aeromonas infections in hospitalized patients.aeromonas species were isolated from specimens other than faeces from 59 hospital inpatients over a 15 year period. of the isolates, 79.7% were regarded as clinically significant, with skin and soft tissues and blood cultures as the commonest sites of infection. of the isolates, 52.5% were hospital-acquired, and 55.9% of patients had serious underlying disease. community-acquired infections in previously healthy individuals accounted for only 13.6% of isolates. however unlike many other opportun ...19957499821
proteolytic activity of aeromonas caviae.aeromonas strains produce a variety of virulence factors including proteases. studies on the kinetics of growth of aeromonas caviae nrrl b-966 and its proteases suggest that the proteolytic activities are produced throughout the growth phase, with peak level occurring at stationary phase. a. caviae synthesize both intracellular and extracellular proteases with the latter account for major portion of the total activity. optimum ph for the a. caviae proteolytic activity is at 7.0. a. caviae produc ...19957473065
recombinant infectious hematopoietic necrosis virus and viral hemorrhagic septicemia virus glycoprotein epitopes expressed in aeromonas salmonicida induce protective immunity in rainbow trout (oncorhynchus mykiss).fragments of the glycoprotein genes of viral hemorrhagic septicemia virus (vhsv) and infectious hematopoietic necrosis virus (ihnv) were cloned into a bacterial broad-host-range expression vector under the control of the plac promoter. western blot (immunoblot) analysis with monoclonal antibodies specific to the glycoproteins demonstrated the inducible expression of the fusion proteins in escherichia coli. aeromonas salmonicida is the causative agent of furunculosis in salmonid fish. it was conf ...19957486994
soil and sediment bacteria capable of aerobic nitrate respiration.several laboratory strains of gram-negative bacteria are known to be able to respire nitrate in the presence of oxygen, although the physiological advantage gained from this process is not entirely clear. the contribution that aerobic nitrate respiration makes to the environmental nitrogen cycle has not been studied. as a first step in addressing this question, a strategy which allows for the isolation of organisms capable of reducing nitrate to nitrite following aerobic growth has been develope ...19957487017
indications and complications of medicinal leech therapy. 19957490356
a novel integron-like element carrying the metallo-beta-lactamase gene blaimp.a plasmid-mediated metallo-beta-lactamase gene was cloned from a carbapenem-resistant serratia marcescens strain, ak9373. the metallo-beta-lactamase gene was identical to the blaimp, and it was located in the space between an integrase-like gene and an aac(6')-ib-like gene. the deduced amino acid sequence for the putative integrase gene showed considerable identity (60.9%) to that of the escherichia coli integrase reported. sequences similar to the gttrrry and an atypical 59-base element contain ...19957492116
the renal membrane dipeptidase (dehydropeptidase i) inhibitor, cilastatin, inhibits the bacterial metallo-beta-lactamase enzyme cpha.the aeromonas hydrophila ae036 chromosome contains a cpha gene encoding a metallo-beta-lactamase which is highly active against carbapenem antibiotics such as imipenem. here we show that the cpha gene product shares inhibitory similarities with a mammalian zinc peptidase, membrane dipeptidase (mdp; dehydropeptidase i). both enzymes are able to hydrolyze imipenem and are inhibited by cilastatin. the active site similarities of these enzymes are not reflected in any significant primary sequence si ...19957492120
the leucine zipper of aeromonas salmonicida abca is required for the transcriptional activation of the p2 promoter of the surface-layer structural gene, vapa, in escherichia coli.the aeromonas salmonicida abca protein is involved in the synthesis of the o-polysaccharide side-chains on the lipopolysaccharide and is also capable of enhancing the expression of the structural gene for the a-layer, vapa, when cloned into escherichia coli. the p2 promoter of the vapa gene of a. salmonicida was cloned into a promoter probe vector and expression in e. coli was monitored. the expression of p2::lacz was shown to be increased when abca was provided in trans. abca contains an n-term ...19957494486
a transferable multiple drug resistance plasmid from vibrio cholerae o1.ten multiple antimicrobial-resistant isolates of vibrio cholerae o1 isolated from patients in uganda were characterized, and the transferability of resistance to bacteria of diverse origins was investigated. the isolates were toxigenic and belonged to biotype e1 tor, serotype ogawa, and ribotype 8, and possessed a 130-mda plasmid of incompatibility group 6-c. this plasmid, designated prvc1, was shown to confer resistance to trimethoprim (mediated by a dhfri gene), sulfonamides (a suii gene), tet ...19959158776
aeromonas and plesiomonas species as bacterial agents of diarrhoea in urban and rural areas of nigeria: antibiogram of isolates.two thousand four hundred stool samples comprising 1 200 each from diarrhoeal and non-diarrhoeal (control) cases with 600 of each category from urban and rural areas were screened for the prevalence of aeromonas and plesiomonas species in the different groups. thirty (5 pc) and (14,8 pc) of aeromonas species and 14 (2,3 pc) and 46 (7,7 pc) of plesiomonas shigelloides were isolated from urban and rural areas respectively for diarrhoea cases. only eight (1,3 pc) and 18 (3 pc) of aeromonas spp. fro ...19958907605
aeromonas species and plesiomonas shigelloides in diarrhoea in goa.sixty six strains (1.9%) of aeromonas species and 7 strains (0.2%) of plesiomonas shigelloides were isolated from 3484 stool/rectal swabs from cases of diarrhoea during the study period of four years from august 1986 to july 1990 in goa. although the selective medium (ampicillin sheep blood agar) was not used for the isolation of aeromonas, an increase in the rate of isolation of aeromonas from 0.2% in the year 1986 to 6.0% in 1990 was observed. stool samples from 100 controls (patients without ...19958919103
haemolytic activity of aeromonas species isolated in libya.twenty seven aeromonas strains (5a. hydrophila, 8a. sobria and 14a. caviae) isolated from children with diarrhoea and 34 aeromonas strains (9a. hydrophila, 7a. sobria an 18a. caviae) isolated from children without diarrhoea were tested from haemolysin production. the results obtained showed that haemolysin production using human, horse or sheep erythrocytes was significantly associated with a.hydrophila and a sobria but not with a.caviae, regardless of whether these strains were isolated from ch ...19959074783
a comparative study of the bbl crystal enteric/nonfermenter identification system and the biomerieux api20e and api20ne identification systems after overnight incubation.the bbl crystal enteric/nonfermenter (crystal, becton dickinson microbiology systems) is a new multi-test identification system for gram negative rods requiring no oil overlay or addition of reagents. one hundred and three selected gram negative rod isolates from routine clinical specimens were tested in parallel using the appropriate api20e or api20ne (biomerieux) reading after overnight incubation. the isolates included in the study, and the number tested, were as follows: acinetobacter sp, 8; ...19958771156
[serogroups, virulence and hemolytic activity of aeromon as hydrophila which caused fish bacterial septicaemia].a new infected disease called bacterial septicaemia occurred in cyprinid fish throughout china during 1989-1992. a. hydrophila was known as the most important pathogen of this harmful disease. thirty-three strains of a. hydrophila, most isolated from moribund fishes, were serogrouped by tube-agglutination with rabbit antisera. most of the isolates could be grouped into two serotypes, tps-30 and pbjs-76. these two groups could be found not only in zhejiang province but in other province in south ...19958745552
emerging pathogens: aeromonas spp.aeromonas spp. are gram-negative rods of the family vibrionaceae. they are normal water inhabitants and are part of the regular flora of poiquilotherm and homeotherm animals. they can be isolated from many foodstuffs (green vegetables, raw milk, ice cream, meat and seafood). mesophilic aeromonas spp. have been classified following the aerokey ii system (altwegg et al., 1990; carnahan et al., 1991). the major human diseases caused by aeromonas spp. can be classified in two major groups: septicemi ...19958750664
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