Publications

open lung biopsy in mycoplasma pneumoniae pneumonia.	pathologic descriptions of mycoplasma pneumoniae pneumonia in humans are scarce, and most descriptions are from autopsy studies. we studied open lung biopsy specimens from six patients with proved or probable m pneumoniae pneumonia and reviewed the findings on m pneumoniae pneumonia that have been reported in the literature, as well as the electron microscopic characteristics of the organism, to document the morphologic spectrum. five of our six cases showed a typical but nonspecific polymorphon ...	1986	3753567
in vivo transfer of antibiotic resistance to a strain of salmonella arizonae.	two trials were conducted to determine the transferability of antibiotic resistance in vivo between two strains of enteric bacteria. newly hatched turkey poults were inoculated per os with a strain of escherichia coli resistant to kanamycin, tetracycline, and ampicillin, a strain of salmonella arizonae resistant to nalidixic acid and streptomycin, or both strains. kanamycin was added to the drinking water of some poults. to test for s. arizonae cells which had received transferable resistance de ...	1986	3703777
[bacillus gordonae sp. nov., a new species belonging to the second morphological group, degrading various aromatic compounds].	thirty strains were isolated from pasteurized soil samples by enrichment culture in aerobiosis at 32 degrees c in a minimal medium containing one of the following compounds as sole source of carbon and energy: quinate, p-hydroxybenzoate, phthalate, isophthalate or trimellitate. these bacteria were rods (0.8 x 2-7 micron), motile by peritrichous flagella. endospores were oval (1.4-1.8 x 2 micron) and distinctly swelled the sporangia. the gram reaction was variable but the gram type was positive. ...	1986	3674781
the effect of arylazido aminopropionyl atp (anapp3) on inhibition of pelvic nerve evoked contractions of the cat urinary bladder.	previously reported work has provided evidence that arylazido aminopropionyl atp (anapp3), a p2-receptor antagonist, blocks the effects of atp and other purine analogs, in the urinary bladder of the cat. this antagonism appeared limited to those effects mediated by p2-receptors, such as contraction of the urinary bladder. however, it was noted that anapp3 did alter the effects of adenosine, mediated through p1-receptors, in some preliminary experiments. a series of experiments was undertaken to ...	1986	3753940
preparation of [3h]collagen for studies of the biologic fate of xenogenic collagen implants in vivo.	reduction of a commercially available, pepsin-solubilized, bovine dermal collagen (vitrogen 100) with sodium [3h]borohydride provided radiolabeled collagen preparations with specific activities ranging from 7.1-12.0 muci/mg collagen. these specific activities were 2-3 times greater than those obtained by reduction of intact rat tail tendon collagen under similar conditions. the alpha, beta, and higher aggregate components of type i collagen were radiolabeled as well as the alpha component of a s ...	1986	3711680
effects of sex steroids on the development of two granule cell subpopulations in the rat accessory olfactory bulb.	the effects of postnatal male castration on day 1 (d1) after birth and female androgenization on accessory olfactory bulb (aob) light and dark granule cell populations were studied. control males showed a greater number of both light and dark aob granule cells than females. postnatal treatment reversed these differences in the light granule cells. female androgenization on d1 does not affect the number of aob dark granule cells. however, male orchidectomy also on d1 significantly decreases the n ...	1986	3779445
[experiments to overcome lactation anestrus in dairy sheep].	an experiment was carried out with three groups of ewes (f1 crosses--black face plevenska x east friesian) used for milk to overcome the lactation anestrus. use was made of megestrol acetate and pms during the mating season. the animals of the 1st (control) group were dried off one month prior to the service-period. those of the iind group were dried off at the beginning of the insemination period; and those of the iiird one were milked during the season and up to the third month of pregnancy. t ...	1986	3727389
directionality and alignment of the foveal receptors, assessed with light scattered from the human fundus in vivo.	the directionality of the foveal receptors of four subjects is assessed with a technique based on light scattering, and a psychophysical technique. the width and orientation of the absorbance diagram (difference between the scattering diagram in a bleached and unbleached state of the visual pigment) are compared with the stiles-crawford function. results show that the directionality in the scattering diagram points into approximately the same direction as the s-c function, but the width of the s ...	1986	3727414
[virulence, viability and antibiotic sensitivity of the causative agent of plague growing on nutrient media at 28 and 37 degrees c].	a comparative study of virulence, viability and antibiotic sensitivity of y. pestis strains grown at 28 degrees c and 37 degrees c in yeast-casein medium, yeast medium with hottinger's meat digest and yeast medium with protein hydrolysate obtained from sunflower seed groats has been made. these media have been found to be suitable for the prolonged cultivation of y. pestis at 28 degrees c and 37 degrees c, for the determination of its sensitivity to antibiotics, as well as for the preservation o ...	1986	3727879
multiple lung cavities in a 12-year-old girl with bubonic plague, sepsis, and secondary pneumonia.	after one week of nonspecific symptoms, pneumonia and an extremely tender and enlarged cervical lymph node developed in a 12-year-old girl who lived in an area of new mexico known to have plague-infected rodents. cultures from an aspirate of the node, her sputum, and blood all showed growth of yersinia pestis. she was treated successfully with aminoglycosides and tetracycline. as the pneumonia resolved, areas of consolidation were replaced by cavitary lesions.	1986	3728514
a microplate enzyme-linked immunosorbent assay for measuring antibody to anaplasma marginale in cattle serum.	an enzyme-linked immunosorbent assay (elisa) method is described for measuring antibody against anaplasma marginale in cattle serum. this method was more sensitive and objective than a previously described elisa method for a. marginale and possible reasons for this are discussed. all 83 cattle experimentally infected with a. marginale (81) or a. centrale (2) developed demonstrable specific antibody but the serums of 98.8% of 839 cattle from cattle tick-free areas did not react by elisa; 378 seru ...	1986	3729834
failure of norfloxacin to eradicate chlamydia trachomatis in nongonococcal urethritis.	norfloxacin has some activity in vitro against chlamydia trachomatis and ureaplasma urealyticum, although not at levels attainable in serum. in this study, norfloxacin was administered (400 mg orally twice daily for 10 days) to men with acute nongonococcal urethritis. of 25 men from whom c. trachomatis was initially isolated, 21 had the organism reisolated at the first follow-up visit posttreatment, and there were minimal changes in the number of inclusion-forming units in culture. ultimately, a ...	1986	3789692
a drug interaction study of ceftriaxone and frusemide in healthy volunteers.	ceftriaxone, a recently developed cephalosporin significantly reduced the diuretic activity of frusemide in rats. for this reason and because an interaction of unknown mechanism is well established between frusemide and some cephalosporins, we studied the interference of ceftriaxone with the diuretic effect of frusemide in healthy volunteers. twelve subjects received frusemide (40 mg p.o.) or placebo in combination with ceftriaxone (2 g i.v.) or saline on 4 different days (cross-over, randomized ...	1986	3733274
a drug interaction study of ceftriaxone and frusemide in healthy volunteers.	ceftriaxone, a recently developed cephalosporin significantly reduced the diuretic activity of frusemide in rats. for this reason and because an interaction of unknown mechanism is well established between frusemide and some cephalosporins, we studied the interference of ceftriaxone with the diuretic effect of frusemide in healthy volunteers. twelve subjects received frusemide (40 mg p.o.) or placebo in combination with ceftriaxone (2 g i.v.) or saline on 4 different days (cross-over, randomized ...	1986	3733274
increased 5-hydroxytryptamine and norepinephrine release from rat brain slices by the red sea flatfish toxin pardaxin.	the effect of the red sea flatfish toxin pardaxin was examined on k+-evoked and on basal release of either [3h]norepinephrine or [3h]5-hydroxytryptamine from preloaded rat cortical slices. the k+-induced release of the neurotransmitters was stimulated in a dose-related manner at concentrations ranging from 0.5 to 4 micrograms/ml. basal release of the two transmitters was elevated to a lesser extent. although the stimulation of evoked release was approximately equivalent for the two neurotransmit ...	1986	3734799
effect of training on plasma anabolic and catabolic steroid hormones and their response during physical exercise.		1986	3744643
aldehyde-induced inhibition of dna repair and potentiation of n-nitrosocompound-induced mutagenesis in cultured human cells.		1986	3749045
pathology of the vertebral column of horses with cervical static stenosis.	specimens of ligamentum flavum, joint capsule, and dorsal lamina were collected at surgery or necropsy from 25 horses with cervical static stenosis. all horses had myelographic evidence of dorsal compression of the spinal cord caused by soft tissue and/or bone in the caudal cervical area, primarily at c6-7. most horses also had radiographic evidence of degenerative joint disease of articular facets. histologically 19 horses had osteosclerosis and cartilage retention in the dorsal lamina, and 24 ...	1986	3750733
myocardial mechanics in allylamine-induced myocardial fibrosis.	to examine the effect of fibrosis on myocardial mechanics, we studied isolated left ventricular papillary muscles from 18 rats given 0.1% allylamine, an agent known to cause myocardial fibrosis, in drinking water for 4-8 wk. six control rats were given tap water. left ventricular hydroxyproline concentration was higher in the allylamine-treated group [3.47 +/- 2.12 vs. 2.10 +/- 0.66 (sd) micrograms/mg dry wt; p less than 0.01]. because of variable and heterogeneous involvement of the ventricle b ...	1986	3752275
evidence of a role for calmodulin in the regulation of calcium release from skeletal muscle sarcoplasmic reticulum.	the effect of calmodulin and calmodulin inhibitors on the "ca2+ release channel" of "heavy" skeletal muscle sarcoplasmic reticulum (sr) vesicles was investigated. sr vesicles were passively loaded with 45ca2+ in the presence of calmodulin and its inhibitors, followed by measurement of 45ca2+ release rates by means of a rapid-quench-millipore filtration method. calmodulin at a concentration of 2-10 microm reduced 45ca2+ efflux rates from passively loaded vesicles by a factor of 2-3 in media conta ...	1986	3754148
nucleotide sequence of a full-length cdna for mouse cytoskeletal beta-actin mrna.		1986	3754329
[changes in the characteristics of glucocorticoid hormone binding in human peripheral blood lymphocytes during myocardial infarct].	competitive binding was used to determine the number of binding sites to glucocorticoid hormones in peripheral blood leukocytes of normal subjects, patients with unstable angina pectoris and patients with myocardial infarction. a sharp (2-3-fold) increase in the number of binding sites was observed in patients with myocardial infarction during the first 24 hours after the onset of the disease.	1986	3801148
yersinia enterocolitica inguinal lymphadenitis.	inguinal lymphadenitis is associated with a well-defined group of etiologic agents including many sexually transmitted diseases and nonvenereal agents including yersinia pestis (bubonic plague). we report herein the first case of a second yersinia species--yersinia enterocolitica--presenting like bubonic plague with bilateral inguinal lymphadenitis.	1986	3757476
acute desensitization to angiotensin ii: evidence for a requirement of agonist-induced diacylglycerol production during tonic contraction of rat aorta.	a possible role for protein kinase c during the tonic phase of arterial contraction was examined in rat aorta by observing the effects of the phorbol ester, 12-o-tetradecanoylphorbol-13-acetate (tpa), on angiotensin ii (aii)-induced responses. the ability of aii and phenylephrine (pe) to induce diacylglycerol (dag) production was monitored as agonist-stimulated 32p-labelling of phosphatidic acid (pa). aii (5 x 10(-7) m) causes only a transient contractile response, while pe (10(-5) m) causes a s ...	1986	3758157
possible role of protein phosphorylation in the mitogenic effect of high density lipoproteins on cultured vascular endothelial cells.	the implication of protein phosphorylation in the mitogenic action of high density lipoproteins (hdl) on bovine vascular endothelial cells was investigated by incubating endothelial cell cultures in the presence of 32p-labeled phosphoric acid. the incorporation of 32p into proteins was measured after fractionation by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and autoradiography of the gel. in endothelial cells seeded at low density and made quiescent by serum s ...	1986	3486870
interactions between host cells and yersiniae.		1986	3426159
replacement of 5-methylcytosine by cytosine: a possible mechanism for transient dna demethylation during differentiation.	in an earlier study it was discovered that when friend erythroleukemia cells (felc) were exposed to a variety of chemical agents capable of inducing differentiation, their dna underwent genome-wide transient demethylation. in an attempt to elucidate the biochemical mechanism responsible for this phenomenon we have induced felc with 5 mm hexamethylenebisacetamide and labeled the dna in vivo with a density label, 5-bromodeoxyuridine, and a radioactive label, deoxy[5-3h]cytidine. newly replicated d ...	1986	3458243
promotion of tubulin assembly by carboxyterminal charge reduction.		1986	3460425
the bacteriology of sheep periodontitis with special reference to actinbacillus actinomycetemcomitans.		1986	3466409
prostate cancer--some aspects of epidemiology, risk factors, treatment and survival.	in western populations prostate cancer was probably rare in the past, yet in many populations the mortality rate has risen 3-5-fold since 1910. the disease now affects 1 man in 20. while the incidence in third world populations living traditionally remains low, it increases with urbanization and prosperity, as in south african blacks. while the disease is age-related, more common in married than in single men and family-orientated, knowledge of specific aetiological factors is meagre. abnormal h ...	1986	3510459
dog inactive renin: biochemical characterization and secretion into renal plasma and lymph.	it has been suggested that the dog is a useful model for studies of inactive renin (ir). however, the nature and origin of the trypsin-activated angiotensin-forming activity in dog plasma have not been fully defined. we characterized dog ir using renin-specific antibody, immunoaffinity, and affigel blue chromatography. activated ir resembles renal and plasma active renin in biochemical and immunological properties. ir was detected in renal lymph, arterial and venous plasma, as well as renal extr ...	1986	3510560
t-2 toxemia and brain prostaglandins.	t-2 toxin is a trichothecene mycotoxin which is a member of a family of closely related sesquiterpenoids. it was recently shown that t-2 toxemia is associated with elevated plasma levels of eicosanoids. to study further the effect of t-2 on the cyclooxygenase pathway of arachidonate we examined the release of pge2, txb2 and 6-keto-fgf1 alpha from brain tissue exposed to t-2 toxin in vivo or in vitro. administration of t-2 toxin (0.75 or 2 mg/kg) to conscious rats caused a transient increase in t ...	1986	3457405
activation of the complement system by cryptococcus neoformans leads to binding of ic3b to the yeast.	the complement system plays a key role in resistance to cryptococcosis. in the present study, we examined several factors that influence the binding of c3 cleavage fragments to cryptococcus neoformans. binding of c3 was determined by using normal human serum supplemented with 125i-labeled c3. incubation of encapsulated cryptococci in 20% serum led to the binding of approximately 3.2 x 10(6) molecules of c3 to each cell. the binding of c3 was markedly inhibited by heating the serum at 56 degrees ...	1986	3514450
comparative antibacterial activity of an arylglycyl oral cephalosporin, ly164846.	ly164846 is a semisynthetic arylglycyl cephalosporin which can be absorbed orally. it had in vitro activity comparable to that of cefaclor against beta-hemolytic streptococcal species and was two- to fourfold more active than cephalexin. enterococci and listeria species were resistant, and its activity against staphylococci was similar to that of other oral cephalosporins. although some bacteroides species were inhibited, the mics for 25% were greater than or equal to 16 micrograms/ml. ly164846 ...	1986	3486632
active smoking depresses prostaglandin synthesis in human gastric mucosa.	to determine the effect of smoking on gastroduodenal mucosal prostaglandin synthesis, endoscopies were done after an overnight fast on 10 nonsmokers, 12 active smokers who smoked four cigarettes in the hour before endoscopy, and then 11 of the smokers who refrained from smoking for 12 hours. biopsy samples of fundic, antral, and duodenal mucosae were incubated, and the accumulation of prostaglandin e2 and 6-keto-prostaglandin f1 alpha in the incubation medium was measured by radioimmunoassay. we ...	1986	3457547
effect of transcutaneous electrical nerve stimulation on pain perception threshold and pain tolerance level of human teeth subjected to electrical stimulation.		1986	3488206
the distribution of vip-immunoreactive neurons in the visual cortex of adult rabbits and during postnatal development.	in the adult rabbit, the majority of the vip-immunoreactive neurons are distributed in layer ii-iii of the visual cortex. labeled cells are also seen in layers vi, iv, v and occasionally in layer i. during postnatal development, we can see labeled neurons on the 2nd day of life, and in layers ii-iii-iv-v-vi on the 4th day. on the 7th day, these neurons reach a distribution typical in the adult animal. from the 2nd to the 10th day the number of vip-neurons increases, and from the 10th day to matu ...	1986	3518861
application of enzyme immunoassays for the confirmation of clinically suspect plague in namibia, 1982.	an outbreak of plague occurred in ovamboland, northern namibia, late in 1982. blood cultures, sera and blood clots were tested to obtain laboratory confirmations for clinically suspect cases of the disease. isolation of the bacillus (yersinia pestis) was attempted from blood cultures; sera were tested for antibody by passive haemagglutination (pha) and enzyme-linked immunosorbent assay (elisa). sera and clots also were tested by elisa for the specific f1 plague antigen. all the elisa procedures ...	1986	3492308
plasmids and pestilence--biological and clinical aspects of bubonic plague.		1986	3521095
gardnerella vaginitis--a guide to identification and management for the practitioner.		1986	3485708
genetic mapping of eleven spo genes essential for ascospore formation in the fission yeast schizosaccharomyces pombe.	sporulation-deficient mutants of the fission yeast schizosaccharomyces pombe were isolated from a homothallic strain mutagenized with ethyl methanesulfonate. complementation tests defined two new genetic loci (spo19 and spo20) essential for ascospore formation, in addition to the 18 known spo loci (bresch et al. 1968). a novel mapping procedure using random spore analysis prior to tetrad analysis allowed us to map 11 spo genes. four genes (spo3, spo15, spo19 and spo20) were mapped on chromosome ...	1986	3442824
chlamydial infections.		1986	3536193
studies on the mechanics of the normal human middle ear.	the middle ear was studied in temporal bone preparations using a laser-doppler interferometer. for measurements at a sound level of 80 db spl this method proved to be very reliable, as was shown by good reproducibility of results in experiments over more than 6 hours. the vibrations of the tympanic membrane and stapes footplate were studied from 200 hz to 10 khz and the results demonstrate a piston-like movement of the stapes footplate up to 120 db spl. the damping effect of the normal ear is lo ...	1986	3536194
functional roles of human class ii antigens in t cell proliferative response. comparison of the dr and dq molecules using mouse monoclonal antibodies.	the monoclonal antibody (mab) nc1 recognizing a monomorphic dr determinant and the anti-dq mabs plm2, plm9, and plm12 recognizing polymorphic dq determinants were produced. nc1 reacted with the h1l1 (dr) and h1l2(drw52, drw53 and allelic products) complexes of a dr family, while the specificity of plm12 was dqw3 (previously called tb21). both plm2 and plm9 reacted with the allodeterminant of ta10 (a subtype of dqw3). these three mabs immunoprecipitated the same h2l3 complex of a dq family. with ...	1986	3497988
[evaluation of vaccinal immunogenesis by the peroxidase method].	the possibility of evaluating functional immunomorphogenesis in the course of the vaccinal process after the injection of conjugated and live brucellosis vaccines, as well as conjugated plague antigen and yersinia pestis strain ev, to guinea pigs has been shown by means of the direct and two-layer variants of the immunoperoxidase assay. the dynamics of the accumulation of globulin-producing cells in the immunocompetent organs and the time course of immunoglobulin titers in the peripheral blood a ...	1986	3548171
[a histopathological investigation of the effects of hypercupremy on bone healing].		1986	3505435
intestinal bacteria translocate into experimental intra-abdominal abscesses.	experimental intra-abdominal abscesses were initiated by surgical implantation of a fibrin clot contaminated with either bacteroides fragilis, bacteroides thetaiotaomicron, or b fragilis-escherichia coli. seven days after surgery the numbers of bacteroides (per gram) in b fragilis and b thetaiotaomicron abscesses were typically log10 8.4 +/- 0.5 (n = 6) and log10 6.4 +/- 0.6 (n = 4), respectively; b fragilis-e coli abscesses typically contained log10 8.9 +/- 0.5 b fragilis and log10 7.6 +/- 0.6 ...	1986	3510604
percutaneous intracardiac direct-current shocks in dogs: arrhythmogenic potential and pathological changes.	in 12 dogs, a total of 65 direct-current (dc) shocks of 100-300 j were delivered through a standard usci6f tripolar electrode catheter to selected sites in the heart. severe arrhythmias were more frequent after electric shocks of high energy to the ventricles and av-nodal or his-bundle region than after comparable shocks to the left or right atria. there was a direct relationship between the strength of the electric shocks, and the extent and severity of the injury. application of 300 j shocks l ...	1986	3488215
altered estrogen action in the senescent rat uterus: a model for steroid resistance during aging.	the estrogen stimulated rat uterus serves as a useful model to examine steroid resistance during aging. this system exhibits receptor loss, impaired stimulation of rna polymerase ii and defects in nuclear translocation (or enhanced association) of receptor-estradiol complexes. all of these defects appear to contribute in part to decreased estrogen responsiveness of the senescent rodent uterus.	1986	3521224
[mathematical modelling of the interaction of the plague capsular antigen with antibodies of varying affinity].	the group of high affinity antibodies is advantageous in antigen binding because of different dissociation rates of the antigen-antibody complexes. an antigen interacts with such a group in accordance with the value of the parameters characterizing the group and the antigen. the unbound antigen interacts with the subsequent group by the affinity value and so on. this is the basis of the presented model of univalent antigen interaction with antibodies of different affinity. the model values of th ...	1986	3521467
formation of behaviorally active estrogen in the dove brain: induction of preoptic aromatase by intracranial testosterone.	the preoptic area (poa) of the male ring dove is a target for specific behavioral effects of estrogen that are separable from those of androgen. activity of the aromatase system in the poa, which converts testosterone to 17 beta-estradiol (e2), is increased by systemic androgen. using crystalline steroid implants positioned stereotaxically in the brain, we examined whether aromatase induction is a result of direct steroid action on the poa, which can occur independently of the behavioral effects ...	1986	3526169
formation of behaviorally active estrogen in the dove brain: induction of preoptic aromatase by intracranial testosterone.	the preoptic area (poa) of the male ring dove is a target for specific behavioral effects of estrogen that are separable from those of androgen. activity of the aromatase system in the poa, which converts testosterone to 17 beta-estradiol (e2), is increased by systemic androgen. using crystalline steroid implants positioned stereotaxically in the brain, we examined whether aromatase induction is a result of direct steroid action on the poa, which can occur independently of the behavioral effects ...	1986	3526169
an escherichia coli rna polymerase tight-binding site on t7 dna is a weak promoter subject to substrate inhibition.	a specific escherichia coli rna polymerase tight-binding (tb) site on bacteriophage t7 has been located at 32,988 base pairs from the left end of t7. this site is referred to as the t7 f promoter since it is fully active in vitro. kinetics of association and dissociation have been measured by use of the abortive initiation assay and runoff transcription. the association constant, ka approximately 9 x 10(5) m-1 s-1, is of the same magnitude as ka for the t7 minor promoters. in competitive titrati ...	1986	3535875
pharmacokinetics of insulin following intravenous and subcutaneous administration in canines.	studies were conducted to examine the absorption and disposition kinetics of insulin in dogs following intravenous (iv) and subcutaneous (sc) administration of commercial preparations. after iv and sc dosing, the plasma levels were described by models which considered basal insulin level contributions. intersubject variation in the disposition kinetics was small with half-lives of 0.52 +/- 0.05 h and total body clearances of 16.21 +/- 2.08 ml min-1 kg-1. calculated insulin plasma secretion rates ...	1986	3535917
distribution of two urinary ribonuclease-like enzymes in human organs and body fluids.	in order to determine the distribution of two human urinary rnase (rnase us and rnase ul)-like enzymes in human tissues and body fluids, enzyme immunoassay systems were established using rabbit anti-rnase sera. the sensitivity of the assay systems was of similar order to that of radioimmunoassay systems previously reported. in the enzyme immunoassay, the cross reactivities of anti-rnase ul serum towards rnase us, bovine kidney rnase k2, bovine rnase a, and bovine seminal rnase vs were less than ...	1986	3514589
distribution of two urinary ribonuclease-like enzymes in human organs and body fluids.	in order to determine the distribution of two human urinary rnase (rnase us and rnase ul)-like enzymes in human tissues and body fluids, enzyme immunoassay systems were established using rabbit anti-rnase sera. the sensitivity of the assay systems was of similar order to that of radioimmunoassay systems previously reported. in the enzyme immunoassay, the cross reactivities of anti-rnase ul serum towards rnase us, bovine kidney rnase k2, bovine rnase a, and bovine seminal rnase vs were less than ...	1986	3514589
growth-associated protein, gap-43, a polypeptide that is induced when neurons extend axons, is a component of growth cones and corresponds to pp46, a major polypeptide of a subcellular fraction enriched in growth cones.	growth-associated protein, gap-43, is a polypeptide that is induced in neurons when they grow axons. we show by means of subcellular fractionation and immunohistochemical localization that gap-43 is a component of neuronal growth cones as well as growing neurites; it is similar to a major phosphoprotein, pp46, of a growth cone-enriched subcellular fraction. these conclusions are consistent with the possibility that the induction of gap-43/pp46 is an important event in the establishment of a prod ...	1986	3517863
lysosomal membrane stabilization by enfenamic acid.		1986	3583334
divinylsulphone-activated agarose. formation of stable and non-leaking affinity matrices by immobilization of immunoglobulins and other proteins.	divinylsulphone-activated agarose is an attractive alternative to several of the activated supports usually used. unlike cnbr-activated gels, it does not leak the immobilized protein at high ph. it reacts readily with proteins at near-neutral ph (unlike the epoxy-activated supports). generally, divinylsulphone-activated agarose reacts with amino, hydroxyl, and sulphydryl groups, thus allowing immobilization of a wide spectrum of ligands. moreover, it is available in an aqueous suspension free of ...	1986	3519637
evolution of a model of estrogen action.		1986	3526452
[effects of acute hypoxemia on fetal movement in the fetal lamb].	chronically instrumented pregnant models were established using 5 dorset-rambouillet pregnant ewes with gestational age between 120 and 138 days. observation of fetal movements were started on the 3rd postoperative day or later when recovery from the surgery to the physiological condition was confirmed by maternal and fetal biophysical parameters. fetal movements were observed using real-time ultrasound equipment with linear-array transducer placed on the maternal abdomen. observation encompasse ...	1986	3543167
agglutination and mating activity of the mf alpha 2-encoded alpha-factor analog in saccharomyces cerevisiae.	the mf alpha 2-encoded asn-5,arg-7 alpha-factor-like peptide has been shown shown to have similar activity to gln-5,lys-7 alpha-factor in morphogenesis and growth arrest studies (s. raths, p. shenbagamurthi, f. naider, and j. m. becker, j. bacteriol. 168:1468-1471, 1986). we tested the asn-5,arg-7 peptide in agglutination and mating assays and found that its activity was similar to or slightly less than that of the gln-5,lys-7 alpha-factor. the asn-5,arg-7 alpha-factor-like peptide is thus the m ...	1986	3536884
agglutination and mating activity of the mf alpha 2-encoded alpha-factor analog in saccharomyces cerevisiae.	the mf alpha 2-encoded asn-5,arg-7 alpha-factor-like peptide has been shown shown to have similar activity to gln-5,lys-7 alpha-factor in morphogenesis and growth arrest studies (s. raths, p. shenbagamurthi, f. naider, and j. m. becker, j. bacteriol. 168:1468-1471, 1986). we tested the asn-5,arg-7 peptide in agglutination and mating assays and found that its activity was similar to or slightly less than that of the gln-5,lys-7 alpha-factor. the asn-5,arg-7 alpha-factor-like peptide is thus the m ...	1986	3536884
[plasmids of the pathogenicity of yersinia pestis].		1986	3537763
the calcium dependence region of the 47 megadalton plasmid of yersinia pestis is required for growth within macrophages.	yersinia pestis cells bearing the 6,47 and 61 megadalton (mdal) plasmids have been shown previously to be more resistant to novobiocin and to take up less gentian violet than cells lacking the 47 mdal plasmid. mutants with lesions in the calcium dependence region of the 47 mdal plasmid, which are known to have resistance to high levels of novobiocin, were found to have rates of gentian violet and deoxycholate sensitivities identical to cells with intact plasmids. cells bearing the 6, 47 and 61 m ...	1986	3564899
effect of the virulence associated 47 megadalton plasmid of yersinia pestis on permeability to gentian violet and sensitivity to novobiocin.	the loss of the 47 megadalton (mdal) plasmid of yersinia pestis resulted in increased gentian violet uptake and increased sensitivity to novobiocin. the addition of calcium caused a decrease in gentian violet uptake and a decrease in novobiocin sensitivity independent of the plasmid. differentiation of cells bearing and lacking the plasmid was accomplished using novobiocin in the presence of calcium at 26 degrees c. the novobiocin resistance levels of cells lacking the 47 mdal plasmid and cells ...	1986	3564897
uromodulin, an immunosuppressive protein derived from pregnancy urine, is an inhibitor of interleukin 1.	uromodulin, an 85-kda glycoprotein isolated from pregnancy urine, has been shown to inhibit antigen-induced proliferation of human lymphocytes in vitro. the present investigation was undertaken to determine its mechanism of action. uromodulin was found to be a potent inhibitor of interleukin 1 (il-1)-induced thymocyte proliferation. uromodulin was compared to a previously described 30- to 35-kda il-1 inhibitor isolated from urine of febrile patients (febrile inhibitor). uromodulin and the febril ...	1986	3538018
[kinetics of the immunologic agglutination reaction and express determination of bacteria using an automated laser photon-correlation spectrometer].		1986	3516614
uromodulin, an immunosuppressive protein derived from pregnancy urine, is an inhibitor of interleukin 1.	uromodulin, an 85-kda glycoprotein isolated from pregnancy urine, has been shown to inhibit antigen-induced proliferation of human lymphocytes in vitro. the present investigation was undertaken to determine its mechanism of action. uromodulin was found to be a potent inhibitor of interleukin 1 (il-1)-induced thymocyte proliferation. uromodulin was compared to a previously described 30- to 35-kda il-1 inhibitor isolated from urine of febrile patients (febrile inhibitor). uromodulin and the febril ...	1986	3538018
a permeability mutant of yersinia pestis with increased susceptibility to phagocytosis which retains potential for intraphagocytic growth and virulence.	gentian violet resistance was used to select mutants of yersinia pestis with altered cell envelope permeability. mutants in one class lacked the 6 and 61 megadalton (mdal) plasmids, but retained the 47 mdal plasmid associated with calcium dependence. mutants in a second class retained all three plasmids. one mutant in the latter class, ev76s7, lacked major outer membrane protein j and yielded reduced levels of minor outer membrane proteins a and c. protein j is known to interact differently with ...	1986	3564898
development of highly immunogenic variants of a rat fibrosarcoma line during in vitro cultivation.	rat fibrosarcoma kmt-17 cells decreased in tumorigenicity when cultured in vitro. eight clones derived from cultured kmt-17 cell lines (c-kmt-17) were examined for their tumorigenicity, immunosensitivity, and immunogenicity. all the clones were less or nontumorigenic in normal syngeneic rats than kmt-17 cells maintained in vivo. all eight clones produced tumors in rats immunosuppressed with 600 rad 60co; differences in degree of tumorigenicity were seen among clones in rats irradiated with 250 r ...	1986	3632916
comparative evaluation of identification systems for testing methicillin-resistant strains of staphylococcus aureus.	several commercial systems are available to distinguish between staphylococcus aureus and the coagulase-negative species of the micrococcaceae family. four latex agglutination systems (accu-staph, serostat, staphaurex, and staphylatex) and two hemagglutination systems (hemastaph and staphyloslide) were compared for their performance in the rapid identification of 232 isolates of staphylococci, including 114 of methicillin-resistant s. aureus. accu-staph, staphaurex, and staphyloslide correctly i ...	1986	3638308
toxicity of essential oils on toxigenic and nontoxigenic fungi.		1986	3697529
evidence for the presence of a novel biosynthetic pathway for norspermidine in vibrio.	enzymatic studies of the cell extracts of vibrio alginolyticus and v. parahaemolyticus provided evidence that there exists a novel biosynthetic pathway for norspermidine (nh2(ch2)3nh(ch2)3nh2), a major polyamine species. in this pathway, the schiff base formed between aspartic beta-semialdehyde and 1,3-diaminopropane is first reduced by a nadph-dependent enzyme to yield "carboxynorspermidine" (nh2(ch2)3nh(ch2)2ch(nh2)cooh), which is in turn decarboxylated by a pyridoxal phosphate dependent enzym ...	1986	3697846
further investigation of the effect of chronic ethanol on the electric membrane properties of adult drg neurons in cell culture.	neural cell cultures were used to investigate the effects of acute and chronic ethanol exposure on electric membrane properties (emp) of adult mouse dorsal root ganglion (drg) neurons. these results in combination with results from additional experiments involving chronic exposure to hyposomotic medium provided further support for the hypothesis that the abnormal emp caused by chronic ethanol exposure involved neuronal membrane adaptation to an ethanol-induced membrane expansion.	1986	3714130
[incidence of ureaplasma urealyticum and mycoplasma hominis in persons examined for sterility].		1986	3719732
renal function in the fetal lamb: a chronic model to study physiological effects of ureteral ligation and deligation.	between 53 and 98 days of gestation 11 fetal lambs were subjected to unilateral ureteral ligation. in 8 fetuses re-exploration was performed 35 to 41 days after ligation. chronic catheterization of the obstructed kidney, bladder and fetal vascular system was used for analysis of physiological data. average creatinine clearance was 1.35 +/- 0.067 ml. per minute in the control kidney and 0.226 +/- 0.057 ml. per minute in the obstructed kidney after diversion. free water clearance, osmolar clearanc ...	1986	3723669
relating extracellular potentials and their derivatives to anisotropic propagation at a microscopic level in human cardiac muscle. evidence for electrical uncoupling of side-to-side fiber connections with increasing age.	elucidation of the mechanisms of cardiac conduction disturbances leading to reentry will require resolution of the details of multidimensional propagation at a microscopic size scale (less than 200 micron). in practice, this will necessitate the combined analysis of extracellular and transmembrane action potentials. the purpose of this paper is to demonstrate the relationships between the time derivatives of the extracellular waveforms and the underlying action potentials in the experimental ana ...	1986	3719925
nucleotide sequence of the enterotoxin b gene from staphylococcus aureus.	the complete nucleotide sequence of the enterotoxin b gene from staphylococcus aureus, as well as the 5' and 3' flanking regions, was determined. starting from an atg initiator codon, an open reading frame encoded the enterotoxin b precursor that consisted of 266 amino acids (mr, 31,400). the 5' terminal portion of the gene encodes a signal peptide 27 amino acids long. the deduced amino acid sequence matched, with a few exceptions, the published amino acid sequence of enterotoxin b. the structur ...	1986	3957869
retinal constraints on orientation specificity in cat visual cortex.	most retinal ganglion cells (levick and thibos, 1982) and cortical cells (leventhal, 1983; leventhal et al., 1984) subserving peripheral vision respond best to stimuli that are oriented radially, i.e., like the spokes of a wheel with the area centralis at the hub. we have extended this work by comparing directly the distributions of orientations represented in topographically corresponding regions of retina and visual cortex. both central and peripheral regions were studied. the relations betwee ...	1986	3958796
[the systematic evaluation of medical procedures. apropos of the effectiveness of parallel medicine].		1986	3961360
cross-reactivity of a monoclonal antibody directed against an estrogen-induced protein in mcf-7 human breast cancer cells with murine leydig cell tumor proteins.	an estrogen-responsive murine leydig cell tumor (m5480a) was examined for the presence of cross-reactive proteins to a monoclonal antibody directed against a mr 24,000 estrogen-regulated protein in human breast cancer cells. human breast tumor biopsies were used as controls for the cytosol preparations, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and western blot conditions used in these experiments. the estrogen-regulated mr 24,000 protein was detected in sodium dodecyl sulfate-p ...	1986	3940199
fate of n-(2-hydroxypropyl)methacrylamide copolymers with pendent galactosamine residues after intravenous administration to rats.	n-(2-hydroxypropyl)methacrylamide (hpma) copolymers bearing galactosamine residues accumulate in the liver after intravenous administration to rats (duncan, r., kopecek, j., rejmanová, p. and lloyd, j.b. (1983) biochim. biophys. acta 755, 518-521). in this study hpma copolymers bearing pendent galactosamine residues (1.0-11.6 mol%) were injected intravenously into rats and their rates of blood clearance and liver accumulation were measured. a level of substitution of 4 mol% was found to be suffi ...	1986	3942780
temperature-controlled plasmid regulon associated with low calcium response in yersinia pestis.	both the low calcium response and virulence in yersinia pestis strain kim5 are mediated by genes located on the 75.4-kilobase plasmid pcd1. the results presented here demonstrate the existence of two new genetic loci of pcd1 whose expression is regulated in response to temperature. levels of transcription in the trta and trtb loci were elevated 12- to 16-fold at 37 degrees c compared with levels of transcription at 30 degrees c. in addition, the absolute levels of transcription were the highest ...	1986	3944056
the addition of nitrous oxide to halothane decreases renal and splanchnic flow and increases cerebral blood flow in rats.	thirteen male sprague-dawley rats were anaesthetized with halothane and catheters were placed in the femoral artery and left ventricle. the animals breathed spontaneously through a tracheostomy throughout the investigation. after the surgical preparation, the inspired halothane concentration was maintained at 1.5 vol% (f/o2 = 0.3). after a 30-min stabilization period, strontium-85 radiolabelled microspheres (15 +/- 1 micron) were injected to the left ventricle for determination of cardiac output ...	1986	3942673
plasmid-determined cytotoxicity in yersinia pestis and yersinia pseudotuberculosis.	yersinia pestis kim5 was found to be cytotoxic for the ic21 and p388d1 mouse macrophage cell lines, as well as for resident peritoneal macrophages from c57bl/6 mice. affected cells phagocytosed kim5 inefficiently, became spherical, detached readily from culture dishes, and retained 51cr poorly. the cytotoxic effect was dependent on the presence of the 75-kilobase plasmid pcd1. because this plasmid also encodes the low calcium response (lcr), three mu d1 insertion mutants previously shown to be l ...	1986	3949380
binding of 4(5)-[2-(4-azido-2-nitroanilino)ethyl]imidazole to histamine receptors in guinea pig cerebral cortical membranes.	the interaction of 4(5)-[2-(4-azido-2-nitroanilino)ethyl]imidazole (aah), a photolabile histamine receptor antagonist, with the binding of histamine, mepyramine, and tiotidine to guinea pig cerebral cortical membranes was examined to evaluate the specificity of aah for histamine h1 and h2 receptors. saturable, specific binding of [3h]histamine, [3h]mepyramine, and [3h]tiotidine to the membranes was observed. competition assays were used to assess the relative affinity of aah for h1- and h2-recep ...	1986	3952080
international control of smoking and the us experience.	international data demonstrate convincingly that lung cancer death rates follow and parallel cigarette smoking prevalence rates in both men and women. effective control of lung cancer and the many other diseases associated with smoking can be achieved only by continued reductions in smoking prevalence in developed countries and the arrestment of smoking in developing countries. smoking control strategies can take the form of prevention programs, cessation programs, regulatory and legislative mea ...	1986	3956298
does the pattern of tissue mobilization dictate protein requirements?		1986	3957715
bubonic plague in a cat.	bubonic plague was diagnosed in a cat. the diagnosis was made by isolation of bacteria from a ruptured bubo and by demonstration of an antibody titer to yersinia pestis. the cat was treated successfully with amoxicillin and streptomycin. the entire veterinary staff was treated prophylactically with tetracycline.	1986	3957763
human term placenta releases placental protein 10 (pp10) in tissue culture.	placenta, decidua, chorion and amnion were studied to determine the tissue concentration of placental protein 10 (pp10). as measured by radio-immunoassay, all tissues studied were found to contain approximately the same concentration of pp10, in both early and late pregnancy. the release of pp10 was studied in tissue culture, first by using explants of placenta, decidua, amnion and chorion in four preliminary studies. only the placenta released significant amounts of pp10 into the culture medium ...	1986	3811843
[comparative study of the activity of arginase isoenzymes in brain tumors of humans and experimental animals].	isoenzyme spectrum and total activity of arginase were studied in rat brain during growth of transplanted neurinoma and glioma as well as in malignant tissues of human brain. after transplantation of the tumors two peaks of arginase activation were observed within 2-4 days in rat brain tissues and within 16 days in tumoral tissue. positively charged isoenzyme i of arginase was mainly activated but activity of neutral isoenzyme ii was unaltered or slightly decreased. in human brain tumors activit ...	1986	3811280
reduced number of [3h]nicotine and [3h]acetylcholine binding sites in the frontal cortex of alzheimer brains.	nicotinic cholinergic receptors were measured in human frontal cortex using [3h]nicotine and [3h]acetylcholine (in the presence of atropine) as receptor ligands. a parallel marked reduction in number of [3h]nicotine (52%; p less than 0.01) and [3h]acetylcholine (-55%; p less than 0.05) binding was found in the frontal cortex of alzheimer brains (ad/sdat) when compared to age-matched control brains. as a comparison the number of muscarinic receptors was quantified using [3h]quinuclidinyl benzilat ...	1986	3808458
dynamic model of the equine hindlimb during the swing phase.	a dynamic model is developed to describe the swing phase of the hindlimb of a normally walking horse. the limb was represented by four rigid segments constrained to move in a sagittal plane only. the mathematical equations of motion of this four-element pendulum were formulated using lagrange's theorem. the morphometric parameters from the hindlimb segments of 3 horses were determined using high-speed film analysis. five muscle groups were incorporated in the model. muscle activity was derived f ...	1986	3811820
antibacterial activity and ototoxicity in guinea pigs, and nephrotoxicity in rats of arbekacin.	arbekacin (hbk), 1-n[(s)-4-amino-2-hydroxybutyl]-3',4'-dideoxykanamycin b, showed broad antibacterial spectra against gram-positive and gram-negative bacteria including pseudomonas aeruginosa. it was also effective against gentamycin- or tobramycin-resistant bacteria. hbk was resistant to various aminoglycoside-inactivating enzymes except for aac (2') and aac (6')-iv, both of which slowly inactivated it. even at higher dosages (150 mg/kg i.m. or greater, which resulted in some deaths), hbk never ...	1986	3814212
the structure of the cuticle and sheath of the infective juvenile of trichostrongylus colubriformis.	the infective third-stage juvenile of trichostrongylus colubriformis is surrounded by its own cuticle as well as the incompletely moulted cuticle of the second-stage juvenile, which is referred to as the sheath. the sheath comprises an outer epicuticle, an amorphous cortical zone, a fibrous basal zone and an inner electron-dense layer. the basal zone of the sheath consists of three layers of fibres; the fibres are parallel within each layer, but the fibre direction of the middle layer is at an a ...	1986	3799009
plasmid-like properties of the four virulence-associated factors of yersinia pestis.	the virulence-associated factors of yersinia pestis, which determine the abilities to produce pesticin i (pst+), capsular fraction i antigen (fra+), v and w antigen complex (vwa+) and a cell-surface component for adsorption of exogenous pigments (pgm+), were independently eliminated by cultivation of the cells in the presence of acridine orange, ethidium bromide or sodium dodecyl sulfate at a subinhibitory concentration. a virulent y. pestis strain, yreka, harbored at least five extrachromosomal ...	1986	3796319
[mobilization of plasmid determinants of yersinia pestis using the plasmid pka7].	yersinia pestis plasmids having inserted tn-movable elements and determining pesticinogenicity, ca2+-dependence, production of mice toxin and fraction i have been mobilized for conjugal transfer into y. pestis, y. pseudotuberculosis or e. coli cells by plasmid pka7. plasmid determinants have been transferred with the frequencies 10-4-10-7 per donor cell. the plasmid dna of high molecular mass has been identified in transconjugant cells. plasmid mobilization in yersinia pestis is supposed to be d ...	1986	3796653
nucleotide sequence of the gene encoding the matrix protein of newcastle disease virus.	the nucleotide sequence of the gene encoding the matrix (m) protein of the beaudette c strain of newcastle disease virus (ndv) has been determined from overlapping cdna clones. control sequences typical of paramyxovirus mrna start and polyadenylation signals have been identified. assuming that the m gene starts and finishes at these sequences, the m gene is 1241 nucleotides long and encodes one long open reading frame of 364 amino acids, corresponding to a polypeptide of molecular weight 39605, ...	1986	3786143
release of opioid peptides from the spinal cord of rats subjected to chronic pain.	chronic localized pain increased the level of the opioid peptides, dynorphin (dyn), alpha-neoendorphin (aneo), met-enkephalin (met) and mets-enkephalin-arg6-gly7-leu8 (meagl), in the lumbar enlargement of the rat spinal cord. it was accompanied with a reduction of the spontaneous and k+-stimulated release of aneo and meagl from spinal cord slices in vitro and a decreased release of aneo from the spinal cord in vivo. the results indicate that the reduction in the activity of endogenous opioid pep ...	1986	3123959