Publications

[use of the indirect hemagglutination test for the diagnosis and epidemiological analysis of acute intestinal diseases].		1981	6764969
[efficacy of using the indirect hemagglutination test and tsuverkalov's test in the diagnosis of dysentery].		1981	6764972
[use of the indirect hemagglutination and antibody neutralization reactions in the epidemiological investigation of diseases of dysenteric etiology].		1981	6764973
bacteriological and epidemiological approach to prophylaxis for enteric infection. effects of peritoneal cells from immunized animals on the infectivity of shigella bacillus to hela cells.		1981	6759764
cell-mediated immune responses of synovial mononuclear cells to sexually transmitted, enteric and mumps antigens in patients with reiter's syndrome, rheumatoid arthritis and ankylosing spondylitis.	3h-thymidine uptake responses by synovial mononuclear cells to stimulation with sexually transmitted, enteric and mumps antigens were studied in 12 patients with "sexually transmitted reiter's syndrome", 5 with "enteric reiter's syndrome", 5 with rheumatoid arthritis, 4 with ankylosing spondylitis and 10 with "indolent arthritis of one knee." the "sexually transmitted" and salmonella cases were distinguishable by the responses. synovial responses were sometimes marked when peripheral blood respo ...	1981	6785434
[antibacterial properties of amniotic fluid in relation to selected bacterial strains].		1981	6788654
increase in drug resistance among shigella dysenteriae, sh flexneri, and sh boydii.	two thousand three hundred and seventy strains of shigella dysenteriae, sh flexneri, and sh boydii isolated in england and wales from 1974 to 1978 were tested for resistance to 12 antimicrobial drugs. eighty per cent of strains were resistant to one or more drugs, with sulphonamide resistance occurring most frequently. resistance to streptomycin, tetracycline, ampicillin, and chloramphenicol increased during the period, as did the incidence of multiple resistance. most infections due to sh dysen ...	1981	6790085
endotoxin inhibition of macrophage-mediated bone resorption.		1981	6791790
[immunoglobulins a and g in the intestinal secretions of rats in different microbiologic conditions].	experiments were made with 3 groups of inbred cdf (f344) crl rats: 30 germ-free, 30 conventional and 18 germ-free animals infected orally with shigella flexneri 2an 516. secretory iga (s-iga) was isolated from gut secretion of the conventional rats to obtain rabbit antiserum against it. the levels of s-iga, iga and igg in blood serum and gut secretion were determined. it was shown that gut secretion of the germ-free rats contained no less s-iga than that from the conventional rats, at the same t ...	1981	6794663
[antagonism between virulent and avirulent shigella or escherichia coli (author's transl)].		1981	6798142
conjunctivitis and ethmoiditis due to shigella flexneri in an infant.		1981	7009020
[nature of the epidemic process in sonne and flexner dysenteries and the causes of its induction].	a comparative study of the epidemic process in sh. sonnei and sh. flexneri dysentery in different regions of the ussr revealed that the morbidity level of sh. sonnei dysentery changed simultaneously in the regions under study at intervals of 2-3 years. sh. flexneri dysentery showed morbidity rises occurring at intervals 6-8 years, and their occurrence did not coincide with the periods of elevated morbidity in sh. sonnei dysentery. the data obtained in the cohort analysis and in the study of recu ...	1981	6457474
shigella flexneri bacteremia: a case report.		1981	6753357
[effect of mutations imparting resistance to antibiotics having different mechanisms of action on the virulence of shigella flexneri].	the study of the interaction between mutants of sh. flexneri 2a and epithelial hela cells has revealed that in all cases the loss of virulence in the studied groups of mutants, resistant to antibiotics with different mechanisms of action (polymyxin m, mecillinam and neamin) was linked with the loss of the ability of bacteria to penetrate into epithelial cells. these changes are probably the result of disturbances in the structure of external membrane in polymyxin-resistant mutants, in the regula ...	1981	6269334
serological analysis of core oligosaccharides of shigella flexneri serotype 6 and its r mutants lipopolysaccharides.	the core oligosaccharides originating from shigella flexneri 6 s strains and r mutants were examined by the complement fixation inhibition and passive hemolysis inhibition tests using antisera against the complete and partially degraded core regions. strong cross reactivity between shigella flexneri 6, shigella sonnei phase ii and e. coli c core fractions which all are of r1 type was observed. terminal beta-d-glucosyl groups and alpha-d-galactosyl-1,2-alpha-d-galactosyl sequences are essential e ...	1981	6181755
[hundred year anniversary of dentistry; past, present and future].		1981	351442
[diagnosis and treatment of reticulosarcomas of the stomach].		1982	351273
bender gestalt signs as indicants of conceptual impulsivity.	sixty children were individually administered the matching familiar figures test and the bender gestalt test. the mean age of the subjects was 10 years, 11 months. a significant relationship was found between errors on the bender gestalt test and impulsivity. more specifically, increased or decreased loops (figure four or six), change in angulation, loops for circles and circles for dots or dots for circles were all significantly related to children's impulsivity.	1982	351161
paul revere.		1982	351231
[obtaining dysentery and salmonellosis diagnostica for the coagglutination reaction in lyophilized form and the elaboration of a rapid system].		1982	6182376
[immunoelectrophoresis analysis of the antigenic composition of e. coli k12 hybrids which acquired the serological specificity of s. newcastle and s. flexneri].	genetic and immunoelectrophoretic studies confirm earlier data on the presence of 2 specific antigens of acidic nature in s. newcastle; one of them is a specific thermolabile k-antigen responsible for type iv specificity of these bacteria. the data concerning the differences in the genetic determinants controlling the synthesis of o- and k-antigens in s. newcastle have been obtained. s. newcastle o- and k-antigens did not react with s. flexneri in the group serum system 3, 4, which indicates tha ...	1982	6184914
periodontal disease associated with shigella flexneri in rhesus monkeys. clinical, microbiologic and histopathologic findings.		1982	6124590
lipopolysaccharide receptor on rabbit peritoneal macrophages. i. binding characteristics.	the bordetella pertussis endotoxin, labeled with tritium ((3h)-lps), bound irreversibly and nonspecifically to rabbit lung macrophages, but bound reversibly and specifically to both resident and elicited rabbit peritoneal macrophages. the specific binding capacity of the macrophages was saturated with about 3 x 10(4) lps molecules per cell. the binding was inhibited with the homologous unlabeled endotoxin, but not at all with endotoxin from proteus mirabilis, thus assessing ligand specificity. e ...	1982	6278022
involvement of a plasmid in the invasive ability of shigella flexneri.	representative shigella flexneri strains were studied to determine whether plasmids are involved in their virulence. all invasive s. flexneri strains, irrespective of serotype, were found to harbor a large plasmid of approximately 140 megadaltons in size, although some strains carried additional plasmid species. spontaneous variants of strains of serotypes 1, 2, and 5 had lost this 140-megadalton plasmid and had concomitantly become avirulent, i.e., could neither invade hela cell monolayers nor ...	1982	6279518
attachment and ingestion of bacteria by trophozoites of entamoeba histolytica.	entamoeba histolytica trophozoites were found to be very selective in their interactions with bacteria. two principal mechanisms were shown to be responsible for these interactions. certain bacteria, such as a number of escherichia coli and serratia marcescens strains which are known to contain mannose-binding components on their cell surface, bound to mannose receptors on the amoeba membrane. this attachment was markedly inhibited by alpha-methylmannoside (0.5%), especially when the incubations ...	1982	6281192
degradation of intestinal glycoproteins by pathogenic shigella flexneri.	intestinal mucin glycoproteins were examined for their ability to sustain growth of pathogenic shigella. inoculation of germfree cecal mucin glycoproteins with shigella flexneri 4b resulted at 48 h in a 940-fold increase in the enteropathogen concentration. investigation in vitro of enzymatic degradation by the pathogen led to the identification of a blood group b-degrading glycosidase produced by the bacteria. in in vivo experiments, fecal supernatants of mice monocontaminated with s. flexneri ...	1982	6282754
[characteristics of the pkmr plasmids found in shigella flexneri].	the clinical isolate of sh. flexneri 1b, resistant to 5 antibiotics and sulfonamides, has been studied by the method of conjugation and found to have a group of transfer-suppressed pkmr-plasmids: pkmr 204-1 (ap sm tc cm km su), pkmr 204-2 (sm km su), pkmr 204-5 (km su) and pkmr 204-7 (sm tc cm km su), whose molecular weight was 99, 71.2, 73.8 and 59.5 md respectively. the treatment of the plasmids with restriction endonuclease bamhi has revealed that plasmids pkmr 204-2 and pkmr 204-5 are defini ...	1982	6285647
early colonic lesions in experimental shigella infection in rhesus monkeys: revisited.	rhesus monkeys (macaca mulatta), given 3 x 10(8) to 5 x 10(10) shigella flexneri 2a orally, developed signs of acute shigellosis within 24 hours. a diffuse acute colitis was well established at 48 hours. the inflammatory reaction was confined to the mucosa. the submucosa showed only edema. the shigellae were found predominantly in the columnar cells of the surface epithelium, less frequently in those of the crypt, and least frequently in the lamina propria. shigella bacilli invaded the columnar ...	1982	6153000
[characteristics and trends in the development of an epidemic process of sonne and flexner dysentery among preschool children].	the results of studies indicate that the morbidity rates of dysentery among children attending preschool institutions and children brought up at home converged in recent years. this phenomenon was most pronounced among children of the kindergarten age group. at the same time dysentery caused by sh. sonnei and sh. flexnery produced a higher morbidity rate among children attending nursery in comparison with that among children of the same age group brought up at home. group infections in preschool ...	1982	6211010
[manifestations of the cyclic nature of the epidemic process in dysentery].	different forms of dysentery, especially those caused by sh. sonnei and sh. flexneri, have been found to differ considerably in their cyclic recurrence. the development cycles of the epidemic processes of dysentery have an objective character, occur in the presence of any tendencies in the morbidity rate, and depend on the natural factors. thus, the cycles of increase and decrease in morbidity are 3, 6, 9, 12 years for dysentery caused by sh. sonnei and 6, 7, 8 years for dysentery caused by sh. ...	1982	6213120
[l-transforming effect of furazolidone on shigella flexneri cells].	the capacity of furazolidone for l-transformation of shigella flexneri was studied with its incubation on 1.3 per cent placenta serum salt difco agar. it was found that a single exposure of shigella cells to 0.05 microgram/ml of furazolidone resulted in transformation of some of them to the elements characteristic of the l-forms, i.e. spherical and granular forms capable of reproduction on routine nutrient media and possessing pronounced sensitivity to penicillin.	1982	6753734
[uptake of metals into the cadmium and zinc resistant shigella flexneri].		1982	6755006
[bacillary dysentery - 1980].		1982	6755564
[reiter's syndrome during shigella flexneri infection].		1982	6750449
[increased sensitivity of immune macrophages to the cytotoxic action of virulent shigellae].	the in vitro interaction of live bacteria belonging to virulent and avirulent shigella and salmonella strains with peritoneal macrophages obtained from mice immunized by the intragastric administration of these bacteria has been studied. in contrast to salmonella-activated macrophages capable of resisting the intracellular proliferation and the cytopathic action of homologous bacteria, shigella-activated macrophages become more sensitive to the cytopathic action of virulent shigellae. the abilit ...	1982	6750996
common evolutionary origin of chromosomal beta-lactamase genes in enterobacteria.	a 32p-labeled fragment of dna, encoding the major part of the chromosomal ampc beta-lactamase gene of escherichia coli k-12, was used as a hybridization probe for homologous dna sequences in colonies of neisseria gonorrhoeae, pseudomonas aeruginosa, and different enterobacterial species. the ampc probe detected the presence of homologous dna sequences in clinical isolates of e. coli, shigella flexneri, shigella sonnei, klebsiella pneumoniae, salmonella typhimurium, serratia marcescens, and p. ae ...	1982	6802795
[growth dyssymmetry of shigella flexneri 6(762 s) cells].	dyssymmetry in the growth of the initial and secondary shigella cells at early stages of the formation of microcolonies has been revealed by the method of dynamic morphometry in serial photographs obtained by time-lapse microfilming. electron-microscopic autoradiography has allowed revealing dyssymmetry in the assimilation of exogenous h3-thymidine, h3-threonine, h3-asparaginic acid by different halves of shigella cells in the process of their growth and preparation for mytosis. these results su ...	1982	6805178
experimental model of dysentery.	a method of producing experimental dysentery in rabbits has been developed. a prerequisite for this model is the fact that microscopic fungi eliminating biologically active substances are regularly found in the large intestine of man. using a rubber probe, the large intestine of rabbits was preliminarily irrigated with the filtrate of the aspergillus flavus fungus and subsequently, the suspension of a 24-hour culture of dysentery bacteria shigella flexneri was administered. a specific pathologic ...	1982	6806355
laboratory-acquired shigellosis.		1982	6809196
[studies on phagocytosis of shigella flexneri by peritoneal macrophages (author's transl)].		1982	6809853
[tropical diseases treated at the infection observation department of the un armed forces hospital in the middle east during the first and the last (xii) troop change].		1982	6817387
[case of vulvovaginitis due to shigella flexneri 2a].		1982	6818298
[effect of vaccination on the kinetics of the metabolism of radioactive iodine].		1982	6760245
new provisional serovar of shigella boydii.	ten bacterial strains are described that give biochemical reactions characteristic of the genus shigella but do not belong to any of the established or provisional serovars. the strains ferment mannitol and are antigenically indistinguishable. they do not possess any of the group antigens of shigella flexneri, and it is therefore proposed that they be regarded as members of a new provisional serovar of shigella boydii. strain e16553 is designated as the test strain for the new serovar.	1982	6761353
[biological properties of revertant l forms of shigella flexneri].		1982	6763383
gram-negative broth (hajna) for enrichment and isolation of yersinia enterocolitica.		1982	6763428
efficiency of immunoprophylaxis and immunotherapy by live dysentery vaccine administration in children and adults collectivities.		1982	6763515
bacillary dysentery in children below five years of age at the general hospital, manado.		1982	6763669
[antibacterial activity of 2 trimethoprim analogs (methioprim, brodimoprim) on recently isolated shigella].		1982	6764800
early colonic lesions in experimental shigella infection in rhesus monkeys: revisited.	rhesus monkeys (macaca mulatta), given 3 x 10(8) to 5 x 10(10) shigella flexneri 2a orally, developed signs of acute shigellosis within 24 hours. a diffuse acute colitis was well established at 48 hours. the inflammatory reaction was confined to the mucosa. the submucosa showed only edema. the shigellae were found predominantly in the columnar cells of the surface epithelium, less frequently in those of the crypt, and least frequently in the lamina propria. shigella bacilli invaded the columnar ...	1982	6755871
[fiéssinger-leroy-reiter syndrome. critical review propos of a case].		1982	6757850
rhodanese activity: a simple and reliable taxonomic tool for gram-negative bacteria.	the thiosulphate: cyanide sulphurtransferase (rhodanese) test of vandenbergh, bawdon and berk (1979) has been simplified and 2469 strains from a wide variety of sources representing different biochemical, serological or phage-pattern entities were tested. the percentages of rhondanese-producing strains were: escherichia coli 98%, shigella flexneri serovars 1-5%, x and y 0%, other shigellae 73-100%, yersinia spp. 0%, salmonella subgenera i-iv 0%, citrobacter freundi 16%, klebsiella 37%, enterobac ...	1982	6958873
direct demonstration in intestinal secretions of an iga memory response to orally administered shigella flexneri antigens.		1982	7033378
[increased effectiveness of antibiotic therapy with adaptogens in dysentery and proteus infection in children].	the course of the disease and the host immunological reactivity (iga, igm, igg, cphi, igg and igm antibody titers in the hit, blood bactericidic properties) were studied in 258 children aged 0 to 14 year suffering from acute dysentery caused by shigella sonnei and flexneri and enterocolitis of the proteus etiology. 157 patients were treated with monomycin and kanamycin in combination with adaptogens, such as eleuterococcus and echinopanax elatum nakai l. and 101 patients were treated with the an ...	1982	7036851
[epidemiology and prevention of dysentery].		1982	7047217
[effect of b group vitamins on dysentery bacteria activity during stab culture].	the addition of the vitamin b complex into the culture medium enhances the productivity of the process ensuring the yield of biomass. the presence of the vitamins of this complex in the culture media intensifies the synthesis of some macromolecular compounds and prolongs the period of the physiological activity of the population.	1982	7048820
plasmid-mediated invasiveness of "shigella-like" escherichia coli.	invasive escherichia coli is a "shigella-like" microorganism which causes a dysenteric syndrome through invasion of the human colonic epithelium. representative strains of different serotypes were studied in order to determine whether plasmids are involved in their virulence. all invasive e. coli strains, irrespective of serotype, were found to harbour a large plasmid of approximately 140 mdal. spontaneous variants of serotypes o143 and o124 had lost this plasmid and had become avirulent, i.e. c ...	1982	7049041
shigella species from addis ababa: frequency of isolation and in vitro drug sensitivity.	one hundred and five shigella isolates from addis ababa were studied to determine serogroup frequency and in vitro antibacterial drug sensitivity. about 70% of the isolates were shigella flexneri followed by sh. dysenteriae (15%), sh. boydii (10%) and sh. sonnei (5%). all or most of the strains were susceptible to cephalothin, gentamicin, kanamycin, polymyxin b and trimethoprim-sulphamethoxazole. frequencies of susceptibility to ampicillin, carbenicillin and chloramphenicol were, respectively, 7 ...	1982	7057026
antibacterial substance from carica papaya fruit extract.	ripe and unripe carica papaya fruits (epicarp, endocarp, seeds and leaves) were extracted separately and purified. all the extracts except that of leaves produced very significant antibacterial activity on staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa and shigella flexneri. the mic of the substance was small (0.2-0.3 mg/ml) for gram-positive bacteria and large (1.5-4 mg/ml) for gram-negative bacteria. the substance was bactericidal and showed properties of a pr ...	1982	7097295
[local immunity in acute dysentery. ii. the content of antibody-forming cells in the large intestine mucosa in acute flexner dysentery in the dynamics of the disease].	antibody-forming cells were detected in the large intestine of patients with acute flexner's dysentery by means of the modified jerne - nordin method of hemolysis in agar. this method allowed one to determine the classes of immunoglobulins produced by the cells contained in tissue microspecimens obtained by the biopsy of the intestinal mucosa. the maximum amount of antibody-containing cells could be detected on days 7-12 of the disease. the content of antibody-forming cells was shown to depend o ...	1982	7043970
shigella enterocolitis and acute renal failure.	although acute renal failure secondary to infections is relatively common in adult patients, uremia requiring dialysis has not previously been reported in an adult patient with shigella enterocolitis. our patient, infected with s flexneri, had severe renal failure without any evidence of sepsis, rhabdomyolysis, or the hemolytic-uremic syndrome. antibiotic therapy with trimethoprim-sulfamethoxazole appeared to play a role in his eventual recovery.	1982	7041284
adherence of shigella flexneri to guinea pig intestinal cells is mediated by a mucosal adhesion.	guinea pig colonic epithelial cells released by treating sections of the colon with solutions containing edta, dithiothreitol, and citrate avidly adhered shigella flexneri bacteria. separation of the intestinal cells from nonbound bacteria was achieved by differential sedimentation on a percoll gradient. adherence of s. flexneri to the colonic cells was ca2+ (1 mm) and time dependent. the ph optimum was ph 6.2, and almost no attachment (less than 5%) was observed at low temperature (4 degrees c) ...	1982	7040246
an outbreak of campylobacter enteritis--a rheumatological followup survey.	one hundred and thirty individuals with bacteriologically proven campylobacter jejuni enteric infection in a single outbreak were studied. eighty-eight patients had gastrointestinal symptoms. one probable example of reactive arthritis was found. this is a frequency similar to that found in other comparable series of salmonella typhimurium and shigella flexneri infections, suggesting a common pathogenesis for the reactive arthritis associated with these 3 infections.	1983	6842468
artificial carbohydrate antigens: a block synthesis of a linear, tetrasaccharide repeating-unit of the shigella flexneri variant y polysaccharide.	glycosylation of methyl 2,4-di-o-benzoyl-alpha-l-rhamnopyranoside with 2,3,4-tri-o-acetyl-alpha-l-rhamnopyranosyl bromide gave methyl 2,4-di-o-benzoyl-3-o-(2,3,4-tri-o-acetyl-alpha-l-rhamnopyranosyl) -alpha-l-rhamnopyranoside (4) in 93% yield. conversion of 4 into the corresponding glycosyl bromide was accomplished with dibromomethyl methyl ether. under koenigs-knorr conditions, this bromide reacted with 8-(methoxycarbonyl)octyl 2-o-(2-acetamido-4,6-o-benzylidene-2-deoxy-beta-d-glycopyranosyl)- ...	1983	6200226
[the reactive arthritis syndrome. rheumatological limits].	the term reactive arthritis (ra) refers to an inflammatory joint disease in the absence of bacteria in the joint, but which is caused by a distant extra-articular infection. they occur as a result of a variety of infections, which are essentially genital or gastro-intestinal in subjects with a particular genetic predisposition characterized by the presence of the hla-b27 antigen or one of the creg group of antigens (b7 - b27 - bw22 - bw42). the most complete clinical expression of reactive arthr ...	1983	6607499
antibody-dependent cell-mediated antibacterial activity of intestinal lymphocytes with secretory iga.	secretory antibodies of the iga class (siga) are thought to have an important role in the defence against bacteria at mucosal surfaces--the level at which the infectious agents first come into contact with the host. however, the mechanism by which siga exert their antibacterial activity is still a matter of debate. after the recent discovery of receptors for the fc portion of iga (rfc alpha) on lymphocytes, monocytes and granulocytes of human, rabbit, guinea pig and mouse origin, it has been hyp ...	1983	6646200
[transfer of h2s plasmid from e. coli to shigella].		1983	6374333
[effect of plasmids r on the changes in s. flexneri sensitivity to phages].		1983	6369040
[clinico-immunological characteristics of dysentery].		1983	6369561
[antagonism between virulent shigella and avirulent shigella].		1983	6406619
[shigellosis abroad].		1983	6379992
expression of hydroxamate and phenolate siderophores by shigella flexneri.	shigella flexneri strains were assayed for the ability to synthesize and utilize phenolate and hydroxamate siderophores. the hydroxamate aerobactin was synthesized by all isolates tested, whereas phenolates were only rarely produced. expression of aerobactin was accompanied by production of a single iron-regulated outer membrane protein (mr = 74,000). this protein was not produced by a mutant defective in aerobactin utilization and may serve as the aerobactin receptor. phenolate (enterobactin)-p ...	1983	6224775
[clinico-laboratory characteristics of gastroenterocolitic (atypical) dysentery].		1983	6362054
change in phage-sensitivity of shigella flexneri strains. i. changes in virulence and phage-sensitivity.	in the course of repeated passaging of virulent shigella flexneri cultures in liquid media, avirulent variants and variants of reduced virulence appeared. they were easily recognizable by their colonial morphology. the avirulent variants became sensitive to certain phages not lysing the original virulent strains. the change of phage-sensitivity among the variants with no or decreased virulence was significant: chi 2 = 64.25; p less than 0.001. the amplification of phage-sensitivity developed in ...	1983	6362315
[dissociation and phagolytic characteristics of the shigella isolated from patients with various clinical forms of dysentery].	the relationship between the severity of the disease and the phagolysis of the isolated cultures of s. flexneri and s. sonnei has been established. in the moderately severe form of the disease the duration of the latent period of the development of the phage has been found to increase in comparison with that in the mild form of the disease.	1983	6364654
characterization of virulence plasmids and plasmid-associated outer membrane proteins in shigella flexneri, shigella sonnei, and escherichia coli.	the 140-megadalton plasmids of shigella flexneri serotypes 1, 3, and 5, in addition to the 120-megadalton plasmid of shigella sonnei, are associated with virulence. the present study showed that a 140-megadalton plasmid is also associated with virulence in escherichia coli. when these plasmids were cleaved with ecori or bamhi restriction endonucleases, considerable homology was evident in plasmids from s. sonnei strains, whereas only a few common fragments were observed among the s. flexneri and ...	1983	6299962
pms76, a plasmid capable of amplification by treatment with chloramphenicol.	pms76 is a nonconjugative, 5.54-megadalton plasmid. this plasmid is present in escherichia coli k12 cells at about 20 copies per chromosome. in addition, the pms76 plasmid can be mobilized by conjugative plasmids and it shares a number of other properties with the amplifiable cole1 plasmid, including the ability to amplify copy number in the presence of chloramphenicol. however, pms76 is compatible with cole1-like replicons, pbr313, and with other multicopy plasmids, rsf1030 and pacyc184. also t ...	1983	6300947
entamoeba invadens and e. histolytica: separation and purification of precysts and cysts by centrifugation on discontinuous density gradients of percoll.	the different cell forms in the life cycle of entamoeba invadens (trophozoites, precysts, and cysts) were rapidly and quantitatively separated on density step gradients of polyvinylpyrolidone-coated colloidal silica particles (percoll). with this method, the gradual process of encystation by e. invadens trophozoites could be monitored. percoll gradients were also efficient in separating trophozoites of entamoeba histolytica and bacteria. after purification on percoll, trophozoites display no evi ...	1983	6303827
the polymicrobial origin of intestinal infections in homosexual men.	to determine the microbial cause and the clinical and pathologic correlates of anorectal and intestinal symptoms in homosexually active men, we performed comprehensive microbiologic studies, anoscopy, sigmoid-oscopy, and rectal biopsy in men examined in a clinic for sexually transmitted diseases. enteric pathogens were found in 95 of 119 consecutive homosexual men with anorectal or intestinal symptoms and in 29 of 75 randomly selected homosexual men without such symptoms (p less than 0.001). the ...	1983	6308444
[fiessinger-leroy-reiter's syndrome : serological conversion for yersinia enterocolitica type 9, positive serodiagnosis for shigella flexneri and chlamydia].	a case of reiter disease in a hla b27 positive man with urethritis followed by polyarthritis is reported. during the disease, serologic conversion for yersinia enterocolitica type 9 was detected as well as positive serologic tests for shigella flexneri and chlamydia. the features of reactive arthritis related to yersinia and the etiologies of reiter disease are recalled.	1983	6314526
plasmid-determined antibiotic resistance in shigella flexneri isolated in england and wales between 1974 and 1978.	the majority of shigella flexneri strains isolated in england and wales are from infections contracted abroad. most of these strains are drug-resistant, over 75% being resistant to streptomycin and sulphonamides, sulphonamides alone or streptomycin, sulphonamides and tetracyclines. a selection of resistant strains was tested for resistance transfer and the plasmids identified were characterized by compatibility grouping. streptomycin and sulphonamide resistance was usually determined by a non-au ...	1983	6337209
role of heat labile antigens of shigella flexneri in hela cell invasion.	in studies of the role of surface antigens of shigella flexneri in hela cell invasion, three antisera were employed to block the invasion. antisera against live (als) and boiled (abs) s. flexneri blocked invasion very effectively. reduction in the numbers of intracellular shigellae was always accompanied by reduction in the number adherent to the cells, indicating the importance of adhesion in the invasive process. anti-live absorbed antiserum (alas) prepared by exhaustive absorption of als with ...	1983	6337262
an evaluation of ocular inflammation following the injection of bacterial endotoxin into the rat foot pad.		1983	6337969
rapid method for detection, identification, and susceptibility testing of enteric pathogens.	three hundred and seven colonies believed to be enteric pathogens were selected from primary plates of macconkey, xylose desoxycholate, or salmonella-shigella agar for inoculation to lactose-sucrose broth, urea-41 motility medium, modified andrade glucose broth with inverted durham tube, pregrowth broth, triple sugar iron agar, lysine iron agar (lia), and christensen urea agar. the rapid screen consisted of interpreting the lactose-sucrose, urea-41 motility, and modified andrade glucose broth ga ...	1983	6338037
use of uv-irradiated bacteriophage t6 to kill extracellular bacteria in tissue culture infectivity assays.	we have utilized 'lysis from without' mediated by uv-inactivated bacteriophage t6 to eliminate extracellular bacteria in experiments measuring the internalization, intracellular survival and replication of yersinia pestis within mouse peritoneal macrophages and of shigella flexneri within a human intestinal epithelial cell line. the technique we describe has the following characteristics: (a) bacterial killing is complete within 15 min at 37 degrees c, with a greater than 10(3)-fold reduction in ...	1983	6338113
fulminating encephalopathy associated with shigella flexneri infection.	three cases of rapidly fatal encephalopathy associated with shigella flexneri infection are reported. there was a lack of severe intestinal involvement and absence of metabolic derangement. in all 3 patients, areas of necrosis were present throughout the brains; and in one case pontine haemorrhages and demyelination were seen. this report supports the evidence of a particular neurotoxic effect in shigellosis.	1983	6338835
[perforated duodenal ulcer in acute dysentery in a 7-year-old child].		1983	6340055
shigellosis with bacteremia: a report of two cases and a review of the literature.	it is widely believed by both physicians and microbiologists that bacteremia is a rare event in shigellosis. we report two cases of shigellosis with positive blood cultures and review 68 cases reported in the literature between 1963 and 1981. we suggest that detection of bacteremia in shigellosis may be facilitated by a greater awareness of the following findings. 1. eighty-seven percent of all cases of shigellosis with bacteremia reported in the literature during the 18-year period reviewed occ ...	1983	6340078
[effect of a live enteral dysentery vaccine from spontaneous flexner mutant 2a 516m on the immunoglobulin-producing cell count in the large intestine mucosa in dysentery].	the influence of vaccinal therapy with live oral dysentery vaccine prepared from s. flexneri 2a 516m on the content of immunoglobulin-producing cells in the mucous membrane of the large intestine was studied. a considerable increase in the number of iga- and igm-synthetizing cells was shown to occur in the course of the infectious process in acute dysentery. in chronic dysentery the content of iga- and igm-synthetizing cells in patients was considerably lower than in a smooth course of acute dys ...	1983	6340380
[specificity of the antibody neutralization reaction in indicating shigella antigens].	the specificity and sensitivity of the antibody neutralization test in the diagnosis of dysentery have been studied. this test has proved to be highly specific in experimental and clinical trials. the antibody neutralization test is more effective than the methods of the isolation of shigellae. the accuracy of this test can be increased by the elimination of different antierythrocyte agents from the secretions of persons under examination.	1983	6340381
[electron microscopic study of microorganisms grown on nutrient media with a nonnutrient raw material base].	a comparative electron-microscopic study of sh. flexneri 8512, str. dick ia and cor. pseudodiphthericum test strains, grown in experimental culture media prepared from inedible raw materials and in currently used culture media based on fish and meat, has been carried out. the ultrastructure of the test strains grown in culture media prepared from inedible raw materials has been shown to remain unchanged.	1983	6340385
[morphological characteristics of phagolysis of s- and r-forms of shigella flexneri].	the differences in the character and time of the lysis of s. flexneri s- and r-mutant cells have been detected by the method of time-lapse microfilming. the asymmetry of the phagolysis of the s-form of shigellae infected with phage f1 has been revealed.	1983	6340391
alterations in the pathogenicity of escherichia coli k-12 after transfer of plasmid and chromosomal genes from shigella flexneri.	a 140-megadalton plasmid (pwr110), which has previously been associated with virulence in shigella flexneri, was transferred to escherichia coli k-12. segments of s. flexneri chromosomal material were then transferred to the plamid-bearing k-12 strains. the virulence of these transconjugant hybrids was assessed in the hela cell model, in ligated rabbit ileal loops, or in the sereny test. a k-12 strain which harbored only pwr110 invaded hela cells, produced minimal lesions in the rabbit ileal muc ...	1983	6341237
shigella infections in the united states, 1974-1980.	during the seven-year period 1974-1980, 93,516 shigella isolates from humans were reported to the centers for disease control, atlanta, through a nationwide surveillance system. over the past 30 years, the reported incidence of shigellosis has been declining in contrast to that reported for salmonellosis. shigella sonnei (group d) now accounts for approximately 70% of the shigella isolates reported, since most of the decline has been due to the increasingly less frequent infections caused by shi ...	1983	6341480
[clinical characteristics of dysentery caused by shigella flexneri in middle-aged and elderly patients].		1983	6341707
[leukocyte migration activity in patients with dysentery].		1983	6342261
[epidemiology of bacillary dysentery in algeria. i. the epidemiological aspects of dysentery in algeria].	bacterial dysentery plays an important role among infectious diseases in algeria. a tendency to the growth of the morbidity rate has been observed. dysentery is irregularly spread in different zones of the country. the highest morbidity rate is registered in the sahara zone and in the eastern part of the country. the morbidity rate among the urban population is higher than among the rural population. s. flexneri prevail in the etiological structure of dysentery infection. of all age groups, the ...	1983	6342313
multiple enteric pathogens in travelers from endemic areas: case report.		1983	6344046
shigella septicaemia.		1983	6344366
delayed hypersensitivity in murine salmonellosis: specificity of footpad reaction in mice infected with rough mutants of salmonella typhimurium.	delayed type (footpad) hypersensitivity (dth) in balb/c mice immunized with rough mutant strains of salmonella typhimurium lt2 was examined. injection of live organisms of an rb mutant tv148 strain induced dth in mice, while injection of the heat-killed organisms did not. the mice immunized with live organisms of the ra, rb, rc, rd, and re mutant strains showed positive footpad reactions to the heat-killed cell antigen of lt2 (wild type) strain. the mice immunized with the rb mutant strain also ...	1983	6346026