Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| acute and chronic neuropathies: new aspects of guillain-barré syndrome and chronic inflammatory demyelinating polyneuropathy, an overview and an update. | during the last 15 years new information about clinical, electrophysiological, immunological and histopathological features of acute and chronic inflammatory neuropathies have emerged. thus, the guillain-barré syndrome (gbs) is no longer considered a simple entity. subtypes of the disorder besides the typical predominant motor manifestation, are recognized, i.e. a cranial nerve variant with ophthalmoplegia, ataxia and areflexia, an immune-mediated primary motor axonal neuropathy (aman), and a mo ... | 1998 | 9872432 |
| the specificity of antibody in chickens immunised to reduce intestinal colonisation with campylobacter jejuni. | poultry consumption has been identified as a major risk factor for human infection with campylobacter jejuni in developed countries. c. jejuni is present in the gastrointestinal tract of broiler chickens at the time of slaughter, and faecal contamination of carcases during processing results in significant campylobacter loads on carcases. one approach to reducing the level of carcase contamination with c. jejuni is to control campylobacter infection in broiler chickens. to this end, the study de ... | 1998 | 9874102 |
| survival and growth of campylobacter jejuni after artificial inoculation onto chicken skin as a function of temperature and packaging conditions. | campylobacter jejuni is one of the major causes of food poisoning in humans. c. jejuni is also widespread in food animals, and meat and meat products derived from food animals are the most common vector of bacterial transmission to humans. to determine the role of packing and storage conditions on the replication of c. jejuni on chicken, the virulent strain c. jejuni 81116 was artificially inoculated onto chicken skin pieces (1 cm2) and stored at different temperatures and under various packagin ... | 1998 | 9874337 |
| shedding and colonization of campylobacter jejuni in broilers from day-of-hatch to slaughter age. | poultry are considered to be the primary reservoirs of campylobacter jejuni for humans. campylobacter jejuni can colonize the poultry intestinal tract and its subsequent shedding can result in environmental contamination, resulting in an increased risk of infection for the rest of the flock. at present, there is no information on the daily shedding pattern of c. jejuni in broiler chickens. thus, the purpose of this study was to determine the daily shedding pattern of c. jejuni in broiler chicken ... | 1998 | 9876841 |
| characterisation of 16 campylobacter jejuni and c. coli typing bacteriophages. | taxonomic classification of bacteriophages specific for campylobacter jejuni and c. coli has not been reported previously. a set of 16 virulent phages, distinguishable by their lytic spectra, has been used extensively for epidemiological typing of c. jejuni and c. coli at preston public health laboratory. these phages were investigated by electron microscopy, pulsed-field gel electrophoresis and restriction endonuclease analysis. all phages had icosahedral heads and long contractile tails. accor ... | 1998 | 9879954 |
| spirochaete-like swimming mode of campylobacter jejuni in a viscous environment. | the swimming patterns of campylobacter jejuni in environments of low and high viscosity were examined by a video tracking method. in media of low viscosity, c. jejuni swam with an average velocity of 39.3 microm/s with frequent changes in direction. the velocity of c. jejuni increased in a medium at a little higher viscosity than that of a low viscosity buffer. in addition to this, c. jejuni showed a second increase of velocity in media of a high viscosity of about 40 centipoise. the swimming pa ... | 1998 | 9879971 |
| sequence comparison of outer membrane phospholipases a: implications for structure and for the catalytic mechanism. | in this study, the nucleotide sequence of the enterobacter agglomerans plda gene encoding outer membrane phospholipase a (ompla; ec 3.1.1.32) was determined. five other ompla amino acid sequences have previously been described, and screening of data bases of whole genome sequencing projects revealed the presence of proteins with homology to ompla in helicobacter pylori, campylobacter jejuni, yersinia pestis, neisseria menigitidis and neisseria gonorrhoeae. comparison of these eleven ompla amino ... | 1998 | 9921577 |
| a putative adhesin gene cloned from campylobacter jejuni. | thirteen campylobacter jejuni strains of human origin showed differing behaviours when analysed for their ability to bind the caco-2 cell line in vitro, suggesting variations in genetic complements and/or regulation. we designed an oligonucleotide probe corresponding to a highly conserved part of adhesins from various gram-negative bacteria. among our laboratory collection, southern hybridization has demonstrated that only a discrete number of strains harbour this sequence. the corresponding gen ... | 1998 | 9921579 |
| expression of the flgfg operon of campylobacter jejuni in escherichia coli yields an extra fusion protein. | two campylobacter jejuni genes with homology to the escherichia coli flgf and flgg genes encoding two of the basal body rod proteins were isolated, and the nucleotide sequence was determined and analyzed. these two c. jejuni genes were shown, by northern hybridization analysis, to function as a single operon (flgfg). two transcriptional start sites were detected upstream of flgf, corresponding to the two rna transcripts detected in the northern blot. western blot immunoassays using anti-flgf and ... | 1998 | 9931471 |
| campylobacter jejuni bacteremia in an hiv-positive patient with non-hodgkin's lymphoma. | 1998 | 10761086 | |
| campylobacter jejuni diarrhea in north karnataka. | 1998 | 10216571 | |
| host mutations (miaa and rpsl) reduce tetracycline resistance mediated by tet(o) and tet(m). | the effects of mutations in host genes on tetracycline resistance mediated by the tet(o) and tet(m) ribosomal protection proteins, which originated in campylobacter spp. and streptococcus spp., respectively, were investigated by using mutants of salmonella typhimurium and escherichia coli. the miaa, miab, and miaab double mutants of s. typhimurium specify enzymes for trna modification at the adenosine at position 37, adjacent to the anticodon in trna. in s. typhimurium, this involves biosynthesi ... | 1998 | 9449261 |
| cloning and nucleotide sequence of the dna gyrase gyra gene from serratia marcescens and characterization of mutations in gyra of quinolone-resistant clinical isolates. | the sequence of the dna gyrase gyra gene of serratia marcescens atcc 14756 was determined. an open reading frame of 2,640 nucleotides coding for a polypeptide with a calculated molecular mass of 97,460 was found, and its sequence complemented the sequence of an escherichia coli gyra temperature-sensitive mutation. analysis of the pcr products of the quinolone resistance-determining regions of gyra genes from six quinolone-resistant clinical isolates revealed a single amino acid substitution, ser ... | 1998 | 9449286 |
| neutrophil-activating protein mediates adhesion of helicobacter pylori to sulfated carbohydrates on high-molecular-weight salivary mucin. | the in vitro binding of surface-exposed material and outer membrane proteins of helicobacter pylori to high-molecular-weight salivary mucin was studied. we identified a 16-kda surface protein which adhered to high-molecular-weight salivary mucin. this protein binds specifically to sulfated oligosaccharide structures such as sulfo-lewis a, sulfogalactose and sulfo-n-acetyl-glucosamine on mucin. sequence analysis of the protein proved that it was identical to the n-terminal amino acid sequence of ... | 1998 | 9453593 |
| identification of a linked set of genes in serpulina hyodysenteriae (b204) predicted to encode closely related 39-kilodalton extracytoplasmic proteins. | a tandem pair of nearly identical genes from serpulina hyodysenteriae (b204) were cloned and sequenced. the full open reading frame of one gene and the partial open reading frame of the neighboring gene appear to encode secreted proteins which are homologous to, yet distinct from, the 39-kda extracytoplasmic protein purified from the membrane fraction of s. hyodysenteriae. we have designated these newly identified genes vspa and vspb (for variable surface protein). | 1998 | 9440540 |
| epidemiological study of guillain-barré syndrome in south east england. | to determine the incidence, treatment, and outcome of guillain-barré syndrome in south east england. | 1998 | 9436731 |
| detection of toxin-producing cyanobacteria by use of paramagnetic beads for cell concentration and dna purification. | early detection of water blooms caused by potential toxin-producing cyanobacteria is important in environmental monitoring. we present a new nucleic acid-based method for detection of cyanobacteria in water that utilizes the same paramagnetic solid phase (beads) for both bacterial cell concentration and subsequent dna purification. in the cell concentration step, the beads were attracted to a magnet after cell adsorption (in an alcohol- and salt-containing solution), and the supernatant was remo ... | 1998 | 9435059 |
| differentiation of campylobacter isolates on the basis of sensitivity to boiling in water as measured by pcr-detectable dna. | differential sensitivity for the release of pcr-detectable genomic dna upon boiling in water is reported for 45 campylobacter jejuni and campylobacter coli strains isolated in egypt. all of the strains released pcr-detectable dna when treated with proteinase k and sodium dodecyl sulfate. when dna was extracted from these strains by boiling in water, nine (20%) of the strains were pcr negative or resistant to boiling, suggesting the presence of boiling-sensitive and boiling-resistant phenotypes. | 1998 | 9435091 |
| etiology of acute gastroenteritis in hospitalized children in melbourne, australia, from april 1980 to march 1993. | acute infectious diarrhea is common in children. control requires knowledge of causes. few comprehensive long-term studies of etiology have been undertaken in developed countries. this report is of a 13-year survey of 4,637 children from 0 to 14 years of age, admitted to a large children's hospital for treatment of gastroenteritis, in which viruses, bacteria, and parasites were sought. a recognized enteric pathogen was identified in 56.6% of children. group a rotaviruses occurred in 39.6% of chi ... | 1998 | 9431936 |
| the effect of class ii major histocompatibility complex expression on adherence of helicobacter pylori and induction of apoptosis in gastric epithelial cells: a mechanism for t helper cell type 1-mediated damage. | helicobacter pylori infection is associated with gastric epithelial damage, including apoptosis, ulceration, and cancer. although bacterial factors and the host response are believed to contribute to gastric disease, no receptor has been identified that explains how the bacteria attach and signal the host cell to undergo apoptosis. using h. pylori as "bait" to capture receptor proteins in solubilized membranes of gastric epithelial cells, class ii major histocompatibility complex (mhc) molecules ... | 1998 | 9584144 |
| campylobacter jejuni bacteremia in human immunodeficiency virus (hiv)-infected and non-hiv-infected patients: comparison of clinical features and review. | clinical and bacteriologic features and clinical outcomes of culture-confirmed campylobacter bacteremia in 21 patients (including nine human immunodeficiency virus [hiv]-infected subjects and 12 non-hiv-infected subjects) were retrospectively evaluated and compared. this study highlights differences between hiv-infected and non-hiv-infected individuals. campylobacter jejuni bacteremia in hiv-infected patients was found to often be a severe, debilitating febrile illness requiring multiple and pro ... | 1998 | 9455515 |
| antimicrobial activity of novel furanonaphthoquinone analogs. | analogs of furanonaphthoquinone (fnq) from tecoma ipe mart had mics ranging from 1.56 to 25 microg/ml against gram-positive bacteria. fnq showed significantly lower mics against methicillin-resistant staphylococcus aureus than against methicillin-sensitive s. aureus. fnq inhibited helicobacter pylori with an mic of 0.1 microg/ml. fungi, including pathogenic species, were sensitive to fnq with mics similar to those of amphotericin b. | 1998 | 9517956 |
| effect of yeast-supplemented feed on salmonella and campylobacter populations in broilers. | the effect of the yeast, saccharomyces boulardii, on experimental cecal colonization of broilers with salmonella typhimurium and campylobacter jejuni was investigated. duplicate pens of broiler chicks were given ad libitum access to a standard feed supplemented with no yeast (control), or 1 g (1x), or 100 g (100x) dried s. boulardii/kg feed. all chicks except negative controls were challenged on day 4 with 3.2 x 10(8) cfu s. typhimurium and 6.5 x 10(8) cfu c. jejuni by oral gavage. after 3 wk, t ... | 1998 | 9521452 |
| antibodies to gangliosides and galactocerebroside in patients with guillain-barré syndrome with preceding campylobacter jejuni and other identified infections. | the relationship between preceding infections and antibodies to glycolipids was investigated in 205 japanese patients with guillain-barré syndrome (gbs). serological evidence of recent campylobacter jejuni (c. jejuni) infection was found in 45% of the patients, compared with 1% in healthy controls. in contrast, recent infection of cytomegalovirus (cmv), mycoplasma pneumoniae (m. pneumoniae) and epstein-barr virus (ebv) was detected in only 5%, 2% and none of the patients, respectively. c. jejuni ... | 1998 | 9521613 |
| close association of iga anti-ganglioside antibodies with antecedent campylobacter jejuni infection in guillain-barré and fisher's syndromes. | iga has an important function in the gastrointestinal immune system. we investigated iga anti-ganglioside antibodies in guillain-barré syndrome (gbs) and fisher's syndrome (fs) subsequent to campylobacter jejuni enteritis. in previous studies, serological diagnosis of c. jejuni infection was based on the detection of igg, iga, and igm anti-c. jejuni antibodies. our study, however, showed that the detection of igg anti-c. jejuni antibody alone was sufficient for the serological diagnosis of antec ... | 1998 | 9521615 |
| survival of infectious cryptosporidium parvum oocysts in seawater and eastern oysters (crassostrea virginica) in the chesapeake bay. | oocysts of cryptosporidium parvum placed in artificial seawater at salinities of 10, 20, and 30 ppt at 10 degrees c and at 10 ppt at 20 degrees c were infectious after 12 weeks. those placed in seawater at 20 ppt and 30 ppt at 20 degrees c were infectious for 8 and 4 weeks, respectively. these findings suggested that oocysts could survive in estuarine waters long enough to be removed by filter feeders such as oysters. thereafter, 30 eastern oysters, crassostrea virginica, were collected with a d ... | 1998 | 9501446 |
| cloning and characterization of the fur gene from helicobacter pylori. | the fur homologue of helicobacter pylori was isolated by screening a plasmid-based, genomic dna library using the fur titration assay (furta). the analysis of the dna sequence revealed significant homology with fur proteins from various other bacterial species. the highest degree of homology was observed for the fur protein from campylobacter jejuni. the h. pylori fur gene on a plasmid could partially complement the fur mutation in escherichia coli strain h1681. the repressor activity depended o ... | 1998 | 9503612 |
| detection and prevalence of helicobacter infection in pet cats. | the presence of spiral bacteria in the feline stomach has been recognized for over a century, but the identities and degrees of prevalence of such organisms in privately owned cats are still poorly documented. the aims of this study were (i) to adapt different diagnostic tools and evaluate their practicality for diagnosing feline gastric helicobacter colonization, (ii) to determine the prevalence of gastric helicobacter-like organisms in pet cats, (iii) to identify the feline species, and (iv) t ... | 1998 | 9508286 |
| reduction of carriage of enterohemorrhagic escherichia coli o157:h7 in cattle by inoculation with probiotic bacteria. | bacteria inhibitory to escherichia coli o157:h7 were isolated from cattle and evaluated for their potential for reducing carriage of e. coli o157:h7 in calves. eighteen of 1,200 bacterial isolates from cattle feces and intestinal tissue samples were screened and determined to inhibit the growth of e. coli o157:h7 in vitro. seventeen of the isolates were e. coli and one was proteus mirabilis. none produced shiga toxin. genomic dna fingerprinting by pulsed-field gel electrophoresis revealed 13 dis ... | 1998 | 9508288 |
| [toxic megacolon as a complication of campylobacter jejuni enterocolitis]. | we report the case of a previously healthy 53-year-old white male who developed an extraordinary complication of acute campylobacter jejuni colitis. toxic megacolon occurred while the patient was treated with a fluoroquinolone antibiotic and glucocorticoids, which were given for endoscopically suspected crohn's colitis. during the course of the disease no cause of colitis was found other than c. jejuni. despite the extreme dilatation, the patient was treated conservatively with parenteral nutrit ... | 1998 | 9816615 |
| differentiation of campylobacter jejuni serotype o19 strains from non-o19 strains by pcr. | guillain-barré syndrome (gbs), a neurologic disease characterized by acute paralysis, is frequently preceded by campylobacter jejuni infection. serotype o19 strains are overrepresented among gbs-associated c. jejuni isolates. we previously showed that all o19 strains tested were closely related to one another by randomly amplified polymorphic dna (rapd) and restriction fragment length polymorphism analyses. rapd analysis demonstrated a 1.4-kb band in all o19 strains tested but in no non-o19 stra ... | 1998 | 9817874 |
| prevalence of astrovirus infection among chilean children with acute gastroenteritis. | the frequency of astrovirus infection in 456 chilean children with diarrhea was determined by enzyme-linked immunosorbent assay, reverse transcriptase pcr, and cell culture. astrovirus was detected in 16.5% of rotavirus-negative and 7% of rotavirus-positive samples obtained from emergency rooms or hospitals and in 11% of samples from day care centers. hast-1 was the predominant serotype identified. | 1998 | 9817899 |
| toxoplasma gondii-associated guillain-barré syndrome in an immunocompetent patient. | we report a case of guillain-barré syndrome associated with an acute, disseminated toxoplasmosis due to a new and unknown zymodeme (zymodeme 12) in an immunocompetent patient. the patient's condition improved with pyrimethamine and sulfadiazine. only the standard treatment for toxoplasmosis was effective, whereas intravenous immunoglobulins were ineffective. | 1998 | 9817908 |
| genetic contribution of the tumor necrosis factor region in guillain-barré syndrome. | we studied genetic polymorphisms in the tumor necrosis factor (tnf) region in 81 japanese patients with guillain-barré syndrome (gbs) and 85 controls. a significantly higher frequency of the 100-base pair (bp) (tnfa2) allele of the tnfa microsatellite marker, which is associated with high tnf alpha production, existed in campylobacter jejuni-positive (cj+) gbs patients than in controls, suggesting the involvement of a genetic predisposition to high tnf alpha secretion in the development of c. je ... | 1998 | 9818939 |
| a campylobacter outbreak associated with stir-fried food. | an outbreak of gastrointestinal illness affecting 12 of 29 customers of a 'hawaiian' theme restaurant specializing in stir-fried food occurred in cardiff, wales in february 1997. campylobacter jejuni serotype hs50 phage type 49 (pt49) was isolated from 5 cases. a total of 47 isolates of c. jejuni hs50 pt49 were identified from wales during 1997, of which 11 were isolated in late february or early march and from the cardiff area. in the outbreak, illness was associated with eating stir-fried chic ... | 1998 | 9825777 |
| a foodborne outbreak of campylobacter jejuni (o:33) infection associated with tuna salad: a rare strain in an unusual vehicle. | we report a foodborne outbreak of campylobacter jejuni infection in a summer camp. outbreak-related cases occurred in 79 persons including 3 secondary cases in campers. campylobacter jejuni was isolated from stool specimens from 16 of 21 patients who submitted a sample; 13 viable isolates were serotyped and all were serotype o:33 (somatic o scheme) or hl:18 (heat-labile scheme), and biotype iii (lior scheme). this serotype is widely distributed geographically but rarely isolated from humans. sam ... | 1998 | 9825778 |
| measurement of motility of helicobacter pylori, campylobacter jejuni, and escherichia coli by real time computer tracking using the hobson bactracker. | (1) to make precise measurements and comparisons of various aspects of motility of three gastrointestinal pathogens, helicobacter pylori, campylobacter jejuni, and escherichia coli, in log phase growth; (2) to provide background information on motility data to study the influence of ph, viscosity, and chemotactic factors, thereby gaining a better understanding of bacterial pathogenesis. | 1998 | 9828825 |
| molecular characterization of a stress-inducible gene from lactobacillus helveticus. | a gene (htra) coding for a stress-inducible htra-like protein from lactobacillus helveticus cnrz32 was cloned, sequenced, and characterized. the deduced amino acid sequence of the gene exhibited 30% identity with the htra protein from escherichia coli; the putative catalytic triad and a pdz domain that characterize the htra family of known bacterial serine proteases were also found in the sequence. expression of the l. helveticus htra gene in a variety of stress conditions was analyzed at the tr ... | 1998 | 9829922 |
| the bacillus subtilis nucleotidyltransferase is a trna cca-adding enzyme. | there has been increased interest in bacterial polyadenylation with the recent demonstration that 3' poly(a) tails are involved in rna degradation. poly(a) polymerase i (pap i) of escherichia coli is a member of the nucleotidyltransferase (ntr) family that includes the functionally related trna cca-adding enzymes. thirty members of the ntr family were detected in a search of the current database of eubacterial genomic sequences. gram-negative organisms from the beta and gamma subdivisions of the ... | 1998 | 9829937 |
| development of a multiplex pcr gene fingerprinting method using gyra and pfla polymorphisms to identify genotypic relatedness within campylobacter jejuni species. | the two genes gyra and pfla, whose sequence variability had been previously described, were evaluated separately for their potential value in discriminating strains of campylobacter jejuni. a single method was then developed by which the two loci were simultaneously amplified using a multiplex pcr procedure, and banding patterns were generated using a pre-selected set of restriction endonuclease enzymes. the method was applied to 18 strains of camp. jejuni from different poultry sources varying ... | 1998 | 9830118 |
| high frequency of metronidazole resistance among strains of campylobacter jejuni isolated from birds. | a total of 2157 strains of the enteropathogen campylobacter jejuni were examined for resistance to metronidazole (5 mg l-1). high rates of metronidazole resistance, between 82 and 100%, were observed amongst strains of avian origin, including commercially reared broiler chickens (90.1%, n = 314) and turkeys (92%, n = 100), as well as wild birds sampled from farms and coastal environments, such as starlings (82%, n = 50), and gulls (100%, n = 20). in comparison, isolates from bovine and ovine rum ... | 1998 | 9830138 |
| [the relationship of campylobacter jejuni subsp. jejuni enterotoxigenicity and the increase of camp and electrolyte changes in the rat intestine]. | small intestine alterations produced by the enterotoxigenic capacity of campylobacter jejuni subsp. jejuni are similar to the hydric, electrolytic and pathological changes caused by choleraic and thermolabile escherichia coli toxins. | 1998 | 9830743 |
| cloning, sequencing, and characterization of the lipopolysaccharide biosynthetic enzyme heptosyltransferase i gene (waac) from campylobacter jejuni and campylobacter coli. | campylobacter jejuni and campylobacter coli are common causes of gastrointestinal disease and a proportion of c. jejuni infections have been shown to be associated with the guillain-barré syndrome. the waac gene from campylobacter coli, involved in lipopolysaccharide core biosynthesis, was cloned by complementation of a heptose-deficient strain of salmonella typhimurium, as judged by novobiocin sensitivity, lipopolysaccharide (lps)-specific phage sensitivity, and polyacrylamide-resolved lipopoly ... | 1998 | 9831648 |
| [reactive arthritis and erythema nodosum caused by campylobacter jejuni]. | 1998 | 9835163 | |
| high-level resistance to trimethoprim in clinical isolates of campylobacter jejuni by acquisition of foreign genes (dfr1 and dfr9) expressing drug-insensitive dihydrofolate reductases. | the pathogenic bacterium campylobacter jejuni has been regarded as endogenously resistant to trimethoprim. the genetic basis of this resistance was characterized in two collections of clinical isolates of c. jejuni obtained from two different parts of sweden. the majority of these isolates were found to carry foreign dfr genes coding for resistant variants of the dihydrofolate reductase enzyme, the target of trimethoprim. the resistance genes, found on the chromosome, were dfr1 and dfr9. in abou ... | 1998 | 9835491 |
| rapid emergence of high-level resistance to quinolones in campylobacter jejuni associated with mutational changes in gyra and parc. | quinolone resistance in clinical isolates of campylobacter jejuni in sweden increased more than 20-fold at the beginning of the 1990s. resistance to 125 microgram of ciprofloxacin per ml in clinical isolates was associated with chromosomal mutations in c. jejuni leading to a thr-86-ile substitution in the gyra product and a arg-139-gln substitution in the parc product. | 1998 | 9835526 |
| effect of low-osmolality nutrient media on growth and culturability of campylobacter species. | the growth and culturability of campylobacter jejuni nctc 11351 and other campylobacters were examined in media having different osmolalities at a range of temperatures (4, 25, and 42 degreesc). the medium osmolalities used ranged from the osmolality of full-strength nutrient medium (modified campylobacter broth having an osmolality of around 254 mosmol) down to 96 mosmol. the following two methods were used to produce media having different osmolalities: dilution of the nutrient medium with dis ... | 1998 | 9835543 |
| life below the gum line: pathogenic mechanisms of porphyromonas gingivalis. | porphyromonas gingivalis, a gram-negative anaerobe, is a major etiological agent in the initiation and progression of severe forms of periodontal disease. an opportunistic pathogen, p. gingivalis can also exist in commensal harmony with the host, with disease episodes ensuing from a shift in the ecological balance within the complex periodontal microenvironment. colonization of the subgingival region is facilitated by the ability to adhere to available substrates such as adsorbed salivary molecu ... | 1998 | 9841671 |
| the virulence plasmid of yersinia, an antihost genome. | the 70-kb virulence plasmid enables yersinia spp. (yersinia pestis, y. pseudotuberculosis, and y. enterocolitica) to survive and multiply in the lymphoid tissues of their host. it encodes the yop virulon, an integrated system allowing extracellular bacteria to disarm the cells involved in the immune response, to disrupt their communications, or even to induce their apoptosis by the injection of bacterial effector proteins. this system consists of the yop proteins and their dedicated type iii sec ... | 1998 | 9841674 |
| campylobacter fetus surface layer proteins are transported by a type i secretion system. | the virulence of campylobacter fetus, a bacterial pathogen of ungulates and humans, is mediated in part by the presence of a paracrystalline surface layer (s-layer) that confers serum resistance. the subunits of the s-layer are s-layer proteins (slps) that are secreted in the absence of an n-terminal signal sequence and attach to either type a or b c. fetus lipopolysaccharide in a serospecific manner. antigenic variation of multiple slps (encoded by sapa homologs) of type a strain 23d occurs by ... | 1998 | 9851986 |
| campylobacter jejuni in foods. | 1998 | 9861962 | |
| diarrheagenic escherichia coli. | escherichia coli is the predominant nonpathogenic facultative flora of the human intestine. some e. coli strains, however, have developed the ability to cause disease of the gastrointestinal, urinary, or central nervous system in even the most robust human hosts. diarrheagenic strains of e. coli can be divided into at least six different categories with corresponding distinct pathogenic schemes. taken together, these organisms probably represent the most common cause of pediatric diarrhea worldw ... | 1998 | 9457432 |
| identification of campylobacter jejuni promoter sequences. | a promoterless lacz shuttle vector, which allowed screening of promoters by beta-galactosidase activity in campylobacter jejuni and escherichia coli, was developed. chromosomal dna fragments from c. jejuni were cloned into this vector; 125 of 1,824 clones displayed promoter activity in c. jejuni. eleven clones with strong promoter activity in c. jejuni were further characterized. their nucleotide sequences were determined, and the transcriptional start sites of the putative promoters in c. jejun ... | 1998 | 9457862 |
| genomic analysis of clostridium botulinum group ii by pulsed-field gel electrophoresis. | pulsed-field gel electrophoresis (pfge) was optimized for genomic analyses of clostridium botulinum (non-proteolytic) group ii. dna degradation problems caused by extracellular dnases were overcome by fixation of cells with formaldehyde prior to isolation. a rapid (4-h) in situ dna isolation method was also assessed and gave indistinguishable results. genomic dna from 21 strains of various geographical and temporal origins was digested with 15 rare-cutting restriction enzymes. of these, apai, ml ... | 1998 | 9464411 |
| extended survival and persistence of campylobacter spp. in water and aquatic biofilms and their detection by immunofluorescent-antibody and -rrna staining. | in water microcosm experiments, the survival times of campylobacter isolates differed by up to twofold, as determined by culturing; this difference increased to fourfold when particular combinations of temperature and oxygenation were used. the mean survival times were much longer at 4 and 10 degrees c (202 and 176 h, respectively) than at 22 and 37 degrees c (43 and 22 h, respectively). the influence of anaerobiosis on survival time was less dramatic and differed considerably between isolates. ... | 1998 | 9464415 |
| transient increases in colony counts observed in declining populations of campylobacter jejuni held at low temperature. | colony forming unit counts of campylobacter jejuni were serially determined in a variety of microcosms in which growth was not expected. unremitting decline in colony counts occurred in nutrient-free systems, however, transient increases were observed in human faecal emulsions and nutrient media on storage at between 1 and 25 degrees c. the phenomenon, which was more pronounced at lower temperatures, could not be attributed to sampling errors, cell clumping or the influence of minor fluctuations ... | 1998 | 9465398 |
| serotyping scheme for campylobacter jejuni and campylobacter coli based on direct agglutination of heat-stable antigens. | campylobacter is now the most frequently reported cause of gastrointestinal disease in england and wales, yet few isolates are characterized beyond the genus level. the majority of isolates are campylobacter jejuni (90%), with most of the remainder being campylobacter coli. we describe an adaptation of the penner serotyping scheme in which passive hemagglutination has been replaced by detection of heat-stable antigens by direct bacterial agglutination; absorbed antisera are used where appropriat ... | 1998 | 9466737 |
| trna(arg) (fimu) and expression of sef14 and sef21 in salmonella enteritidis. | a tn10 insertion affecting sef14 fimbrial synthesis in salmonella enteritidis was located 13 bp upstream of a gene designated fimu. the 77-bp dna sequence of fimu from s. enteritidis was identical to that of fimu encoding trna(arg) (ucu) from salmonella typhimurium and 96% identical to that of the escherichia coli argu homolog. furthermore, the open reading frame adjacent to and overlapping the 3' end of fimu was similar to the prophage dlp12 integrase gene. the fimu-encoded transcript comigrate ... | 1998 | 9473037 |
| new flagellin-specifying genes in some escherichia coli strains. | data for further development of the flagellar antigen genetics of the species escherichia coli are reported. two new flagellin genes named flla and flma were found in e. coli 781-55, e2987-73, and e223-69, the test strains for e. coli flagellar antigens h44, h55, and h54, respectively (collection of the international escherichia and klebsiella centre of the world health organization, copenhagen, denmark). two alleles of flla were identified that encode flagellar antigens h44 (flla44) and h55 (fl ... | 1998 | 9473055 |
| the haemophilus influenzae htra protein is a protective antigen. | the htra gene from two strains of nontypeable haemophilus influenzae has been cloned and sequenced, and the encoded approximately 46-kda htra proteins were found to be highly conserved. h. influenzae htra has approximately 55% identity with the escherichia coli and salmonella typhimurium htra stress response proteins, and expression of the h. influenzae htra gene was inducible by high temperature. recombinant htra (rhtra) was expressed from e. coli, and the purified protein was found to have ser ... | 1998 | 9488373 |
| mutation in the peb1a locus of campylobacter jejuni reduces interactions with epithelial cells and intestinal colonization of mice. | campylobacter jejuni is one of the leading causes of bacterial diarrhea throughout the world. we previously found that peb1 is a homolog of cluster 3 binding proteins of bacterial abc transporters and that a c. jejuni adhesin, cell-binding factor 1 (cbf1), if not identical to, contains peb1. a single protein migrating at approximately 27 to 28 kda was recognized by anti-cbf1 and anti-peb1. to determine the role that the operon encoding peb1 plays in c. jejuni adherence, peb1a, the gene encoding ... | 1998 | 9488379 |
| campylobacter jejuni 72dz/92 cjac gene coding 28 kda immunopositive protein, a homologue of the solute-binding components of the abc transport system. | screening of the campylobacter jejuni 72dz/92 cosmid gene bank enabled identification of the cjac (camp. jejuni antigen c) gene encoding a highly immunogenic protein of apparent molecular mass 28 kda. gene bank searches indicated significant overall homology of the cjac gene product, mainly to the gram-negative periplasmic solute-binding proteins of the abc transport system which recognize polar amino acids and opines. cjac protein contains the motif lvac at the end of the putative 19 amino acid ... | 1998 | 9489038 |
| an improved physical and genetic map of campylobacter jejuni nctc 11168 (ua580). | campylobacter jejuni is recognized as the major cause of food-borne gastrointestinal disease in the developed world. to facilitate the molecular genetic analysis of this pathogen, an approximately 18-fold redundant tropist3 cosmid library was constructed from c. jejuni nctc 11168 genomic dna. the cosmid library was partially ordered by hybridization to 15 pulsed-field electrophoresis (pfge) restriction fragments. this analysis confirmed the estimated size of the genome to be 1730 kb, but suggest ... | 1998 | 9493386 |
| campylobacter jejuni infection as a cause of the guillain-barré syndrome. | a previously unrecognized sequelum of infection with campylobacter jejuni, a common cause of gastroenteritis, is guillain-barré syndrome (gbs). gbs is the most common cause of acute neuromuscular paralysis; 30% to 40% of cases are preceded by c. jejuni infection. both patient and bacterial characteristics likely play a role in the pathogenesis of c. jejuni-induced gbs. molecular mimicry between the lps of some campylobacters and structures present on the gangliosides of the peripheral nerve may ... | 1998 | 9494837 |
| foodborne diseases. emerging pathogens and trends. | the epidemiology of foodborne diseases is rapidly changing. in the past 15 years, new foodborne pathogens, such as campylobacter jejuni and escherichia coli o157:h7, have emerged as important public health problems. well-recognized pathogens, such as salmonella serotype enteritidis, have increased in prevalence or become associated with new vehicles, and pathogens such as c. jejuni and s. typhimurium are becoming increasingly resistant to antimicrobial agents. evolving trends in foodborne diseas ... | 1998 | 9494839 |
| antibiotic resistance of bacterial litter isolates. | use of antibiotics in subtherapeutic doses as growth-promoting feed additives for animal production is widespread in the u.s. and throughout the world. previous studies by our research group concluded that size fractionation of poultry (broiler) litter followed by storage facilitated reutilization of litter as a soil amendment or bedding supplement. however, litter microbial contamination, including antibiotic-resistant populations, and accumulation of metals and other elements may limit litter ... | 1998 | 9495488 |
| variation in flagellin genes and proteins of burkholderia cepacia. | the majority of isolates of burkholderia cepacia, an important opportunistic pathogen associated with cystic fibrosis, can be classified into two types on the basis of flagellin protein size. electron microscopic analysis indicates that the flagella of strains with the larger flagellin type (type i) are wider in diameter. flagellin genes representative of both types were cloned and sequenced to design oligonucleotide primers for pcr amplification of the central variable domain of b. cepacia flag ... | 1998 | 9495748 |
| helicobacter pylori porcdab and oordabc genes encode distinct pyruvate:flavodoxin and 2-oxoglutarate:acceptor oxidoreductases which mediate electron transport to nadp. | helicobacter pylori, a major cause of human gastric disease, is a microaerophilic bacterium that contains neither pyruvate nor 2-oxoglutarate dehydrogenase activity. previous studies (n. j. hughes, p. a. chalk, c. l. clayton, and d. j. kelly, j. bacteriol. 177:3953-3959, 1995) have indicated that the major routes for the generation of acetyl coenzyme a (acetyl-coa) and succinyl-coa are via pyruvate:flavodoxin oxidoreductase (por) and 2-oxoglutarate:acceptor oxidoreductase (oor), respectively. th ... | 1998 | 9495749 |
| outbreak of campylobacter enteritis associated with cross-contamination of food--oklahoma, 1996. | on august 29, 1996, the jackson county health department (jchd) in southwestern oklahoma notified the oklahoma state department of health (osdh) of a cluster of campylobacter jejuni infections that occurred during august 16-20 among persons who had eaten lunch at a local restaurant on august 15. this report summarizes the investigation of these cases and indicates that c. jejuni infection was most likely acquired from eating lettuce cross-contaminated with raw chicken. this report also emphasize ... | 1998 | 9498294 |
| ribosomal rna gene restriction fragment diversity amongst penner serotypes of campylobacter jejuni and campylobacter coli. | diversity based on ribosomal rna gene-restriction endonuclease digest patterns was detected amongst forty-seven strains of campylobacter made up of 38 strains of campylobacter jejuni and 9 strains of campylobacter coli. restriction digests of chromosomal dna prepared by treating with hae iii were probed with an oligonucleotide specific for campylobacter 16s ribosomal rna genes. seventeen distinct hybridization patterns, each indicating the presence of 2-4 copies of the 16s rrna gene are encoded ... | 1998 | 9528123 |
| pseudomonas aeruginosa invasion and cytotoxicity are independent events, both of which involve protein tyrosine kinase activity. | pseudomonas aeruginosa clinical isolates exhibit invasive or cytotoxic phenotypes. cytotoxic strains acquire some of the characteristics of invasive strains when a regulatory gene, exsa, that controls the expression of several extracellular proteins, is inactivated. exsa mutants are not cytotoxic and can be detected within epithelial cells by gentamicin survival assays. the purpose of this study was to determine whether epithelial cell invasion precedes and/or is essential for cytotoxicity. this ... | 1998 | 9529067 |
| enhancement of susceptibility to shiga toxin-producing escherichia coli o157:h7 by protein calorie malnutrition in mice. | infection with shiga toxin (stx)-producing enterohemorrhagic escherichia coli is increasing among children. in this study, 5-week-old c57bl/6 mice with protein calorie malnutrition (pcm) that had been fed a 5% protein diet for 2 weeks since ablactation were inoculated intragastrically with 2 x 106 cfu of stx-producing e. coli o157:h7. more than 75% of infected mice with pcm died by 10 days postinfection. infected mice with pcm developed neurologic symptoms 5 days after infection, while well-nour ... | 1998 | 9529103 |
| water as a reservoir for campylobacter jejuni infection in cows studied by serotyping and pulsed-field gel electrophoresis (pfge). | the occurrence of campylobacters was studied in the faecal samples of a dairy herd with about 20 animals and in the lake which was their source of drinking water during the grazing period from june to september. of the total of 141 faecal samples studied, 0-21% were found to be positive for c. jejuni at various sampling times throughout the year. more cows were found to be campylobacter-positive in summer or in autumn after the grazing period than after the winter, when the animals were inside a ... | 1998 | 9529995 |
| microbial food borne pathogens. campylobacter jejuni. | campylobacter jejuni is the most common food borne bacterial pathogen and leading cause of food borne disease in humans in the united states and other industrialized nations. approximately four million cases of human campylobacteriosis occur each year in the united states. although the majority of cases consist of limited diarrheal illness, severe sequelae can affect a small portion of patients with campylobacteriosis that may include reactive arthritis and guillain-barré syndrome. animal reserv ... | 1998 | 9532665 |
| primary campylobacter jejuni infection in different mice strains. | campylobacter jejuni is one of the most frequent causes of diarrhoea in man. extra-intestinal manifestations may also occur, particularly in immunocompromised individuals. however, because of the lack of appropriate animal models the pathophysiology and immunological response of the host to c. jejuni infection are still poorly understood. in our laboratory an experimental infection of adult balb/c, c57bl/6 and dba/2 mice has been established. after intraperitoneal injection of 0.5-1x10(9) cfu of ... | 1998 | 9533898 |
| development of quinolone-resistant campylobacter fetus bacteremia in human immunodeficiency virus-infected patients. | campylobacter fetus subspecies fetus has been recognized as a cause of systemic illness in immunocompromised hosts, including relapsing bacteremia in human immunodeficiency virus (hiv)-infected patients. acquired resistance to quinolone therapy, while reported for a variety of bacteria, including campylobacter jejuni, has not been previously documented for c. fetus. two cases of quinolone-resistant c. fetus bacteremia were detected in hiv-infected patients. cloning and nucleotide sequencing of t ... | 1998 | 9534967 |
| a cyanobacterium lacking iron superoxide dismutase is sensitized to oxidative stress induced with methyl viologen but is not sensitized to oxidative stress induced with norflurazon | a strain of synechococcus sp. strain pcc 7942 with no functional fe superoxide dismutase (sod), designated sodb-, was characterized by its growth rate, photosynthetic pigments, and cyclic photosynthetic electron transport activity when treated with methyl viologen or norflurazon (nf). in their unstressed conditions, both the sodb- and wild-type strains had similar chlorophyll and carotenoid contents and catalase activity, but the wild type had a faster growth rate and higher cyclic electron tran ... | 1998 | 9536078 |
| enterobacterial antibodies in chinese patients with rheumatoid arthritis and ankylosing spondylitis. | to study the role of microbial infection in rheumatic diseases. | 1998 | 9536392 |
| occurrence of homologs of the escherichia coli lytb gene in gram-negative bacterial species. | the escherichia coli lytb protein regulates the activity of guanosine 3',5'-bispyrophosphate synthetase i (rela). a southern blot analysis of chromosomal dna with the e. coli lytb gene as a probe revealed the presence of lytb homologs in all of the gram-negative bacterial species examined but not in gram-positive species. the lytb homologs from enterobacter aerogenes and pseudomonas fluorescens complemented the e. coli lytb44 mutant allele. | 1998 | 9537400 |
| identification of a novel enteric helicobacter species in a kitten with severe diarrhea. | a previously undescribed helicobacter sp. was recovered from a cat with severe diarrhea. based upon the absence of any other identifiable cause of diarrhea, this helicobacter may be involved in the development of the disease signs. the organism could not be cultured but was described on the basis of 16s rrna gene sequence analysis and morphology and appeared to be a new species, with helicobacter canis being the most genetically similar species. the presence of a diarrhea-inducing helicobacter i ... | 1998 | 9542907 |
| [pathophysiology and therapeutic approach in inflammatory demyelinating polyneuropathy--recent advances]. | recent advances on pathogenetic mechanism and therapeutic approach for guillain-barré syndrome (gbs) and chronic inflammatory demyelinating polyneuropathy (cipd) were briefly reviewed. as for gbs, an axonal form has been recognized as a clinicopathological variant, in which campylobacter jejuni infection and elevated anti-gm1 antibody tires are frequently observed. other anti-glycolipid antibodies to gq1b, gd1b and gm2 would also be significant as a determinant factor for a clinical phenotype. b ... | 1998 | 9545774 |
| subtyping of campylobacter jejuni penner heat-stable (hs) serotype 11 isolates from human infections. | high resolution molecular subtyping was applied to campylobacter jejuni penner heat-stable (hs) 11 isolates from human infections and other sources. strains were genotyped by restriction fragment length polymorphism analysis involving pcr-based flagellin gene (flaa) profiling with hinfi and ddei, and pulsed-field gel electrophoretic (pfge) profiling with smai and kpni. fla-genes of the strains were highly conserved as most (95%) had the same fla-profile. pfge analysis of smai digests was more di ... | 1998 | 9569002 |
| molecular characterization of a strain-specific sequence in campylobacter jejuni. | campylobacter jejuni strains 81116 and nctc 11392 were shown to contain a region of dna that was not present in other strains. this region was cloned from the chromosome of strain 81116 and the nucleotide sequence determined. it was found to contain an insert of 742 bp which was flanked by direct repeats of 45 bp. although the nature of this sequence is unknown at present, it is clear that it only presents in certain strains of camp. jejuni and it may therefore be useful as an epidemiological to ... | 1998 | 9569692 |
| antimicrobial properties of plant essential oils and essences against five important food-borne pathogens. | the antimicrobial properties of 21 plant essential oils and two essences were investigated against five important food-borne pathogens, campylobacter jejuni, salmonella enteritidis, escherichia coli, staphylococcus aureus and listeria monocytogenes. the oils of bay, cinnamon, clove and thyme were the most inhibitory, each having a bacteriostatic concentration of 0.075% or less against all five pathogens. in general, gram-positive bacteria were more sensitive to inhibition by plant essential oils ... | 1998 | 9569693 |
| a blood-free enrichment medium for growing campylobacter spp. under aerobic conditions. | detection limits for campylobacter jejuni strains jh93 and atcc 29428 in a new blood-free enrichment broth (bfeb) were investigated under aerobic conditions. cultures of camp. jejuni were inoculated into 50 ml bfeb containing 10% food homogenate in 50 ml screw-cap tubes. after 24 h enrichment under aerobic conditions, camp. jejuni were isolated on four selective agar media. the least squares means of the detection limit 50% endpoint (dl50) values were 0.4 (plain bfeb), 0.9 (crabmeat), 1.7 (mushr ... | 1998 | 9569699 |
| subtyping of campylobacter jejuni penner serotypes 9, 38 and 63 from human infections, animals and water by pulsed field gel electrophoresis and flagellin gene analysis. | pulsed field gel electrophoresis and pcr-rflp flagellin gene profiling were used to discriminate 44 isolates of campylobacter jejuni penner heat stable (hs) serotypes 9, 38 and 63 from sporadic human infections and other sources. genomic similarities between hs9 and hs38 strains were demonstrated. hs63 and hs1 strains of camp. jejuni ssp. jejuni were similar but were genomically distinct from camp. jejuni ssp. doylei hs63. the molecular analyses provided a basis for assessing associations betwee ... | 1998 | 9569705 |
| a rapid pcr-based dna test for enterotoxic bacillus cereus. | the occurrence of dna sequences encoding the hemolysin hbla complex and bacillus cereus enterotoxin bcet, which have recently been confirmed as enterotoxins, was studied in bacillus spp. to amplify these dna sequences, pcr primer systems for the b component of hbla and for bcet dna sequences were developed. the results from the amplification of hbla sequences correlated well with results obtained with the b. cereus enterotoxin (diarrheal type) test kit (rpla kit), but not with the results of the ... | 1998 | 9572928 |
| evidence of genomic instability in campylobacter jejuni isolated from poultry. | poultry isolates of campylobacter jejuni derived from a survey of meat processing batches were genotyped by pulsed-field gel electrophoresis (pfge) of chromosomal dna to establish the clonal relationships between single-colony isolates. in the majority of batches studied, one or two genotype patterns predominated. however, in one batch (batch a), 21 single-colony isolates gave 14 different pfge genotypes. the banding patterns obtained with smai were sufficiently different to distinguish between ... | 1998 | 9572956 |
| campylobacter jejuni cytolethal distending toxin causes a g2-phase cell cycle block. | cytolethal distending toxin (cdt) from the diarrheagenic bacterium campylobacter jejuni was shown to cause a rapid and specific cell cycle arrest in hela and caco-2 cells. within 24 h of treatment, cdt caused hela cells to arrest with a 4n dna content, indicative of cells in g2 or early m phase. immunofluorescence studies indicated that the arrested cells had not entered m phase, since no evidence of tubulin reorganization or chromatin condensation was visible. cdt treatment was also shown to ca ... | 1998 | 9573072 |
| coordinated repression in vitro of the divergent fepa-fes promoters of escherichia coli by the iron uptake regulation (fur) protein. | the mechanism involved in transcriptional repression of the fepa-fes divergent promoters of escherichia coli by the fur (ferric uptake regulation) protein has been examined in vitro. this dna region includes a suboptimal and single fur-binding site with two divergent and overlapped -35/-10 hexamers. comparison of transcription patterns generated with runoff experiments in either the presence or the absence of heparin showed that access of the rna polymerase to the principal -35/-10 hexamers was ... | 1998 | 9573216 |
| specificity of rabbit antisera against lipopolysaccharide of acinetobacter. | acinetobacter has been reported to be involved in hospital-acquired infections with increasing frequency. however, clinical laboratories still lack simple methods that allow the accurate identification of acinetobacter strains at the species level. for this study, proteinase k-digested whole-cell lysates from 44 clinical and environmental isolates were investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting with hyperimmune rabbit sera to examine the possibil ... | 1998 | 9574685 |
| association of providencia alcalifaciens with diarrhea in children. | it has been demonstrated in previous studies that providencia alcalifaciens can produce diarrhea by an invasive mechanism. in the present study, p. alcalifaciens was isolated from the stool specimens of 17 of 814 diarrheal children younger than 5 years of age (2.1%) and from those of 4 of 814 matched controls (0.49%) (p = 0.004), indicating that the organism is significantly associated with diarrhea. however, 71% of p. alcalifaciens-positive diarrheal children had simultaneous infections with ot ... | 1998 | 9574723 |
| increased resistance to quinolones in campylobacter jejuni: a genetic analysis of gyra gene mutations in quinolone-resistant clinical isolates. | campylobacter jejuni is a frequent cause of enteritis and sometimes it requires antimicrobial therapy. we have studied the evolution of resistance to nine antibiotics from 1990 to 1994 and investigated how frequently gyra mutations are involved in the acquisition of quinolone resistance. the percentage of chloramphenicol-, clindamycin-, tetracycline- and amoxicillin plus clavulanic acid-resistant strains has remained practically unchanged and erythromycin and gentamicin resistance has decreased, ... | 1998 | 9580533 |
| cloning and characterization of the gene encoding the primary sigma-factor of campylobacter jejuni. | the rpod gene encoding the primary sigma-factor of campylobacter jejuni was amplified from genomic dna with degenerate oligonucleotide primers. the complete gene encodes a polypeptide of 622 amino acids and has a deduced m(r) of 72.6 kda. this polypeptide is 40% identical to the rpod (sigma 70) protein of escherichia coli and has 66% identity with the helicobacter pylori rpod protein. a c. jejuni sigma 70 promoter, not recognized by the e. coli sigma 70 factor, could be activated in this bacteri ... | 1998 | 9595669 |
| cytochalasin-induced actin disruption of polarized enterocytes can augment internalization of bacteria. | cytochalasin-induced actin disruption has often been associated with decreased bacterial internalization by cultured epithelial cells, although polarized enterocytes have not been systematically studied. in assays using confluent polarized ht-29 enterocytes, cytochalasin d appeared to increase internalization of wild-type salmonella typhimurium, proteus mirabilis, and escherichia coli. hela and hep-2 epithelial cells, as well as ht-29 and caco-2 enterocytes, were used to clarify this unexpected ... | 1998 | 9596696 |
| haemophilus ducreyi infection causes basal keratinocyte cytotoxicity and elicits a unique cytokine induction pattern in an in vitro human skin model. | haemophilus ducreyi is the etiologic agent of the sexually transmitted genital ulcer disease chancroid. predominantly a cutaneous pathogen, h. ducreyi is present in chancroid ulcers that are characterized by extensive neutrophil accumulation in intraepidermal lesions accompanied by a mononuclear infiltrate in the dermis. we used an in vitro human skin model composed of foreskin fibroblasts and keratinocytes to examine host skin cell interactions with h. ducreyi 35000. bacteria replicated and per ... | 1998 | 9596767 |
| physical limitations on salmonella typhi entry into cultured human intestinal epithelial cells. | kinetic studies of salmonella typhi invasion of int407 cells at different multiplicities of infection (mois) have revealed a strict physical limitation on s. typhi entry at mois of >/=40. staining of infected monolayers to distinguish intracellular from extracellular bacteria revealed that all monolayer cells are susceptible to infection and that internalized bacteria are typically contained in one to three separate clusters per cell during the first 60 min. scanning and transmission electron mi ... | 1998 | 9596769 |
| helicobacter pylori disrupts epithelial barrier function in a process inhibited by protein kinase c activators. | helicobacter pylori colonizes the gastric mucosa, and the infection is related to the development of diverse gastric pathologies, possibly by directly or indirectly affecting epithelial-cell function. we analyzed the influence of the bacteria on transepithelial electrical resistance (ter) on a model tight epithelium, t84, grown to confluence in permeable filters. h. pylori sonicates produced a dramatic decrease in ter after 1 to 2 h of exposure, while sonicates from other bacteria did not induce ... | 1998 | 9596771 |
| human antibody response to helicobacter pylori lipopolysaccharide: presence of an immunodominant epitope in the polysaccharide chain of lipopolysaccharide. | we have examined the antibody response to helicobacter pylori lipopolysaccharides (lps) in humans. we used sera from patients with gastroduodenal diseases and healthy adults infected or not infected with h. pylori. data from the experiments for antibody binding to lps suggested that the polysaccharide chains from many h. pylori strains showed high immunogenicity in humans. sera from most (above 70%) h. pylori-infected individuals contained immunoglobulin g (igg) antibodies against the polysaccha ... | 1998 | 9596783 |
| enhanced levels of staphylococcus aureus stress protein groel and dnak homologs early in infection of human epithelial cells. | antibodies to staphylococcus aureus heat shock proteins (hsps) are present in the sera of patients with s. aureus endocarditis (m. w. qoronfleh, w. weraarchakul, and b. j. wilkinson, infect. immun. 61:1567-1570, 1993). although these proteins are immunogenic, their role in infection has not been established. we developed a cell culture system as a model to examine the potential involvement of staphylococcal hsps in the initial events of infection. this study supports a model in which a clinical ... | 1998 | 9596786 |