Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| cytokine profile in genital tract secretions from female adolescents: impact of human immunodeficiency virus, human papillomavirus, and other sexually transmitted pathogens. | quantitative enzyme-linked immunosorbent assays were used to measure interleukin (il)-2, il-10, and il-12 in cervical secretions from female adolescents with and without sexually transmitted infections. compared with human immunodeficiency virus [hiv]-negative patients, hiv-positive patients had higher concentrations of il-10 (118.2 pg/ml vs. 34.5 pg/ml; p=.002) and il-12 (175.5 pg/ml vs. 85.1; p=.03). il-2 concentrations were not statistically different. furthermore, genital tract infections we ... | 2000 | 10720516 |
| development, characterization and distribution of adoptively transferred peripheral blood lymphocytes primed by human papillomavirus 18 e7--pulsed autologous dendritic cells in a patient with metastatic adenocarcinoma of the uterine cervix. | we describe a 27-year-old woman with systemic chemoresistant and radioresistant metastatic disease secondary to a recurrence of human papillomavirus (hpv) 18 infected cervical adenocarcinoma of the uterine cervix who received adoptive transfer of peripheral blood t cells stimulated with hpv 18 e7-pulsed autologous dendritic cells (dc). extensive in vitro characterization of the dc-activated t cells derived from peripheral blood mononuclear cells (pbmc) included phenotypic analysis, cytotoxicity ... | 2000 | 10726612 |
| the roles of e6-ap and mdm2 in p53 regulation in human papillomavirus-positive cervical cancer cells. | the p53 tumor suppressor is regulated by the mdm2 oncoprotein through a negative feedback mechanism. mdm2 promotes the ubiquitination and proteasome-dependent degradation of p53, possibly by acting as a ubiquitin ligase. in cervical cancer cells containing high-risk human papillomaviruses (hpv), p53 is also targeted for degradation by the hpv e6 oncoprotein in combination with the cellular e6-ap ubiquitin ligase. in this report, we describe the identification of efficient antisense oligonucleoti ... | 2000 | 10726657 |
| the amyloid beta peptide abeta (25-35) induces apoptosis independent of p53. | apoptosis of neuronal cells apparently plays a role in alzheimer's disease (ad). the amyloid beta (abeta) peptide derived from beta-amyloid precursor protein is found in ad brain in vivo and can induce apoptosis in vitro. while p53 accumulates in cells of ad brain, it is not known if p53 plays an active role in abeta-induced apoptosis. we show here that inactivation of p53 in two experimental cell lines, either by expression of the papillomavirus e6 protein or by a shift to restrictive temperatu ... | 2000 | 10734238 |
| development and assessment of a general theory of cervical carcinogenesis utilizing a severe combined immunodeficiency murine-human xenograft model. | currently, we lack a theoretical explanation for why squamous cell cervical cancer develops predominantly in specific sites (i.e., along the squamocolumnar junction). we therefore implanted human cervical tissues containing the transformation zone in severe combined immunodeficiency (scid) mice and studied morphology, steroid effects, gene expression, and human papillomavirus (hpv) factors. | 2000 | 10739703 |
| 5-fluorouracil-induced apoptosis in cultured oral cancer cells. | chemotherapy is commonly used to treat advanced oral squamous cell carcinoma (scc) and is known to kill cancer cells through apoptosis. our hypothesis states that 5-fluorouracil (5fu) also kills cultured oral epithelial cells through programmed cell death or apoptosis. cultured oral cancer cells were exposed to an optimum dose of 20 mg/ml of 5fu. cells were analyzed for changes in cell cycle distribution and induction of cell death including apoptosis. normal control, human papilloma virus-immor ... | 2000 | 10745178 |
| epstein-barr virus (ebv) genes expression in cervical intraepithelial neoplasia and invasive cervical cancer: a comparative study with human papillomavirus (hpv) infection. | to elucidate a causative role of epstein-barr virus (ebv) for cervical cancer, presence and expression of ebv genes were examined in 31 cervical carcinomas (icc), 23 cervical intraepithelial neoplasias (cin), and 35 normal cervices (ncx). in reverse transcription polymerase chain reaction (rt-pcr) analysis, eber-1 mrnawas expressed in 74% (23/31) of icc, 83% (19/23) of cin, 37% (13/35) of ncx. lmp-1 was expressed in 52% (16/31) of icc, 70% (16/23) of cin, and 23% (8/35) of ncx, and ebna-2 was ex ... | 2000 | 10746674 |
| role of routine human papillomavirus subtyping in cervical screening. | human papillomavirus testing has been evaluated as an adjunct to cervical cytologic screening. there is a well-established association between high-risk malignant subtypes of human papillomavirus infection and the development of neoplastic change. although human papillomavirus infection is a necessary condition for malignant transformation, it is not a sufficient condition. multiple cofactors are necessary for progression to dysplasia and then to invasive cervical cancer. because of the multifac ... | 2000 | 10752510 |
| p53 codon 72 polymorphism and susceptibility to skin cancer after renal transplantation. | infection with human papillomavirus (hpv) is an important risk factor for the development of skin cancer after renal transplantation. it has recently been suggested that degradation of the tumor suppressor gene p53 is an important mechanism for human papillomavirus-induced carcinogenesis. a common genomic polymorphism occurs at codon 72 of the p53 gene, and in vitro the codon 72arg variant appears to be particularly susceptible to degradation. | 2000 | 10755564 |
| advances in cervical screening technology. | the pap smear unquestionably is a successful screening test for cervical cancer. however, recent advances in technology have raised questions regarding whether the conventional pap smear is still the standard of care. this article relates issues of screening and cost-effectiveness to the state of the art in thin layer preparations, cytology automation, human papillomavirus screening, human papillomavirus vaccines, and other cervical screening adjuncts. perhaps nowhere in medicine is clinical dec ... | 2000 | 10757338 |
| human papilloma virus in melanoma biopsy specimens and its relation to melanoma progression. | to evaluate melanoma biopsy specimens for human papilloma virus (hpv) and determine the relation between the presence of hpv, in vitro growth, and clinical progression of melanoma in the patients from whom the biopsy specimens were derived. | 2000 | 10767787 |
| [effects of human papilloma virus types on course of juvenile respiratory papillomatosis and effectiveness of interferon therapy in children]. | identification of the type of human papilloma virus (hpv) was done by means of polymerase chain reaction in 40 children with juvenile respiratory papillomatosis (jrp). adjuvant to surgery interferon treatment was carried out in 18 patients (study group) with severe jrp. the rest 22 patients (control group) with less severe papillomatosis received only surgical treatment. the majority of the patients from both the groups had the virus type 6/11 (72.2 and 77.3% for the study and control groups, re ... | 2000 | 10771610 |
| human papillomavirus type 16 e7 oncoprotein represses transcription of human fibronectin. | the e7 oncoprotein encoded by human papillomavirus (hpv) type 16 repressed the transcription of fibronectin, a key component of the extracellular matrix. this repression, detected in several hpv-positive nontumorigenic and tumorigenic cell lines, was abolished when the cys-x-x-cys repeats in e7 were disrupted. | 2000 | 10775635 |
| human papillomaviruses and vulvar vestibulitis. | to assess the relationship between human papillomavirus (hpv) infection and vulvar vestibulitis syndrome. | 2000 | 10775729 |
| wild-type p53 transactivates the killer/dr5 gene through an intronic sequence-specific dna-binding site. | killer/dr5, a tumor necrosis factor-related apoptosis-inducing ligand (trail) death receptor gene, has been shown to be induced by dna damaging agents and radiation in a p53-dependent manner. although trail is a potential therapeutic agent for cancer, the induction mechanism of its receptors is poorly understood. here we show the identification of three p53 dna-binding sites in the killer/dr5 genomic locus located upstream (bs1; -0.82 kb) of the atg site, within intron 1 (bs2; +0.25 kb downstrea ... | 2000 | 10777207 |
| mutagenesis of the prb pocket reveals that cell cycle arrest functions are separable from binding to viral oncoproteins. | the pocket domain of prb is required for prb to arrest the cell cycle. this domain was originally defined as the region of the protein that is necessary and sufficient for prb's interaction with adenovirus e1a and simian virus s40 large t antigen. these oncoproteins, and other prb-binding proteins that are encoded by a variety of plant and animal viruses, use a conserved lxcxe motif to interact with prb. similar sequences have been identified in multiple cellular prb-binding proteins, suggesting ... | 2000 | 10779361 |
| characterization of pdz-binding kinase, a mitotic kinase. | hdlg, the human homologue of the drosophila discs-large (dlg) tumor suppressor protein, is known to interact with the tumor suppressor protein apc and the human papillomavirus e6 transforming protein. in a two-hybrid screen, we identified a 322-aa serine/threonine kinase that binds to the pdz2 domain of hdlg. the mrna for this pdz-binding kinase, or pbk, is most abundant in placenta and absent from adult brain tissue. the protein sequence of pbk has all the characteristic protein kinase subdomai ... | 2000 | 10779557 |
| a simplified and reliable hpv testing of archival papanicolaou-stained cervical smears: application to cervical smears from cancer patients starting with cytologically normal smears. | the efficacy of four methods to recover dna from papanicolaou (pap)-stained archival cervical smears for optimal detection of human papillomavirus (hpv) dna by gp5+/biogp6+ polymerase chain reaction (pcr) was investigated. two of the methods were based on proteinase k treatment and two based on treatment with guanidinium thiocyanate (gtc). the quality of the dna as measured by pcr assays amplifying different sizes of the beta-globin gene appeared to be superior for the gtc-based assays. using co ... | 2000 | 10780521 |
| widespread elevated expression of the human papilloma virus (hpv)-activating cellular transcription factor brn-3a in the cervix of women with cin3 (cervical intraepithelial neoplasia stage 3). | 2000 | 10781392 | |
| prognostic implication of human papillomavirus infection in squamous cell carcinoma of the lung. | on the subtropical island of okinawa, squamous cell carcinoma (scc), particularly the well-differentiated form, is the most frequent type of lung cancer, while this form is relatively rare on the japanese mainland and in other countries. furthermore, in okinawa, in 1993, 80% of scc cases of the lung were found to be infected with human papillomavirus (hpv). we studied the prognosis of scc of the lung with hpv infection (n = 25) and compared it with non-hpv-infected scc (n = 16). using the kaplan ... | 2000 | 10782464 |
| cancer in husbands of cervical cancer patients. | we used the swedish family-cancer database to analyze the spectrum of cancers diagnosed in husbands of women with in situ or invasive cervical cancer, and we compared these to second carcinogenic events in women presenting with these cancers. our hypothesis was increased cancer susceptibility from human papilloma virus (hpv). when the wives had in situ or invasive cervical cancer, the standard incidence ratios (sirs) for anal cancer in husbands were 1.75 (95% ci = 1.05-2.62) and 1.92 (95% ci = 0 ... | 2000 | 10784257 |
| retinoic acid resistance at late stages of human papillomavirus type 16-mediated transformation of human keratinocytes arises despite intact retinoid signaling and is due to a loss of sensitivity to transforming growth factor-beta. | in our in vitro model of human cell carcinogenesis, normal human foreskin keratinocytes (hkc) transfected with human papillomavirus type 16 dna (hkc/hpv16) progress toward malignancy through several phenotypically defined and reproducible "steps" that include immortalization, growth factor independence (hkc/gfi), differentiation resistance (hkc/dr), and ultimately malignant conversion. while hkc/hpv16 are very sensitive to growth inhibition by all-trans-retinoic acid (ra) at early passages, they ... | 2000 | 10792999 |
| genetic and epigenetic changes in human epithelial cells immortalized by telomerase. | exogenous expression of htert, the catalytic component of telomerase, is sufficient for the immortalization of human fibroblasts but insufficient for the immortalization of human foreskin keratinocytes (hfks) and human mammary epithelial cells (hmecs). these latter cell types can overcome senescence by coexpression of htert and human papillomavirus (hpv) e7 or by expression of htert and loss of p16(ink4a) expression, indicating that the retinoblastoma (rb) pathway, along with a telomere maintena ... | 2000 | 10793065 |
| folding of a dimeric beta-barrel: residual structure in the urea denatured state of the human papillomavirus e2 dna binding domain. | the dimeric beta-barrel is a characteristic topology initially found in the transcriptional regulatory domain of the e2 dna binding domain from papillomaviruses. we have previously described the kinetic folding mechanism of the human hpv-16 domain, and, as part of these studies, we present a structural characterization of the urea-denatured state of the protein. we have obtained a set of chemical shift assignments for the c-terminal domain in urea using heteronuclear nmr methods and found region ... | 2000 | 10794423 |
| p53 codon 72 polymorphism does not affect the risk of cervical cancer in patients from northern italy. | a case-control study was performed to investigate the risk of cervical cancer associated with p53 polymorphism at codon 72, encoding either arginine or proline. it has been recently suggested that the arginine isoform increases the susceptibility to invasive cervical cancer; however, data remain controversial. the polymorphism was examined by both allele-specific pcr and rflp analysis in 101 patients with primary cervical cancer and in 140 healthy women of the same age and from the same geograph ... | 2000 | 10794489 |
| differential cell cycle response of nontumorigenic and tumorigenic human papillomavirus-positive keratinocytes towards transforming growth factor-beta1. | human papillomaviruses (hpvs) are causative agents of a number of malignancies in humans, including cervical cancer. their tumorigenic potential is linked to expression of the viral e6/e7 genes which can interfere with normal cell cycle control by targeting p53, p21waf1, p27kip1, and prb. we show here that nontumorigenic and tumorigenic hpv-positive keratinocytes (hpk) exhibit striking differences in the response of cell cycle regulatory genes towards transforming growth factor beta-beta1. treat ... | 2000 | 10794545 |
| analysis of mutations in the urr and e6/e7 oncogenes of hpv 16 cervical cancer isolates from central china. | high rates of cervical cancer have been reported from parts of china and this may reflect a predominance of cervical infection with particularly aggressive human papillomavirus (hpv) variants. this pcr-based investigation of cervical tumours from sichuan province in central china demonstrated an hpv positivity rate of 88%. hpv 16 was most common (21/34, 61%), followed by hpv 18 (3/34, 9%), while types 33, 45, 58 and 59 were each identified in one specimen. sequencing of up to 1349 bases of the 2 ... | 2000 | 10797293 |
| factor analysis of confocal image sequences of human papillomavirus dna revealed with fast red in cervical tissue sections stained with toto-iodide. | to visualize and localize specific dna sequences by fluorescence in situ hybridization, confocal laser scanning microscopy (clsm) and factor analysis of biomedical image sequences (famis). | 2000 | 10800619 |
| pkn binds and phosphorylates human papillomavirus e6 oncoprotein. | the high risk human papillomaviruses (hpvs) are associated with carcinomas of cervix and other genital tumors. previous studies have identified two viral oncoproteins e6 and e7, which are expressed in the majority of hpv-associated carcinomas. the ability of high risk hpv e6 protein to immortalize human mammary epithelial cells has provided a single gene model to study the mechanisms of e6-induced oncogenic transformation. in recent years, it has become clear that in addition to e6-induced degra ... | 2000 | 10809724 |
| human papillomavirus testing as a screening tool for cervical cancer | 2000 | 10815111 | |
| induction of s phase and apoptosis by the human papillomavirus type 16 e7 protein are separable events in immortalized rodent fibroblasts. | the hpv16 e7 oncoprotein neutralizes several cell cycle checkpoints, favouring the entry of quiescent cells into s phase. this activity is mediated in part by association of e7 with the pocket proteins and consequent activation of e2f transcription factors. in addition, hpv16 e7 protein is able to promote apoptosis. in this study we demonstrate that the ability to induce apoptosis is a common property of e7s belonging to both benign and malignant hpv types. the e7-induced apoptosis is mediated b ... | 2000 | 10822378 |
| screening and treatment of sexually transmitted diseases part 2: trichomonas, human papillomavirus infection, and genital herpes simplex virus. | 2000 | 10823973 | |
| induction of apoptosis in human papillomaviruspositive cancer cells by peptide aptamers targeting the viral e6 oncoprotein. | certain types of human papillomaviruses (hpvs) are closely linked to the development of human cancers. herein, it is shown that intracellular targeting of the hpv16 e6 oncoprotein by e6-binding peptide aptamers resulted in the apoptotic elimination of hpv16-positive cancer cells, whereas hpv-negative cells were not affected. these results provide direct experimental evidence that the hpv e6 oncoprotein has antiapoptotic activity in hpv-positive tumor cells that is required for their survival. th ... | 2000 | 10829072 |
| human papillomavirus testing in women with mild cytologic atypia. | to evaluate the efficiency of human papillomavirus (hpv) testing by hybrid capture ii (digene diagnostics inc., silver spring, md) with regard to detecting biopsy-confirmed cervical intraepithelial neoplasia (cin) or high-grade cin in women with mild atypia, compared with the efficiencies of polymerase chain reaction (pcr), southern blot hybridization, and cytology. | 2000 | 10831974 |
| comparison of human papillomavirus types 16, 18, and 6 capsid antibody responses following incident infection. | the relationship between human papillomavirus (hpv) dna in the genital mucosa and serum igg to hpv-16, -18, and -6 was studied in a cohort of 588 college women. among women with incident hpv infections, 59.5%, 54.1%, and 68.8% seroconverted for hpv-16, -18, or -6, respectively, within 18 months of detecting the corresponding hpv dna. transient hpv dna was associated with a failure to seroconvert following incident hpv infection; however, some women with persistent hpv dna never seroconverted. an ... | 2000 | 10837170 |
| [diagnosis and treatment modalities in sinonasal inverted papillomas]. | today, etiology and pathogenesis of sinunasal inverted papillomas (ip) remain unknown. surgical approaches and extension of surgical resections are still controversial. | 2000 | 10838687 |
| detection of human papillomavirus in cholesteatomas. | the presence of human papillomavirus dna in cholesteatoma may have some role in the development of middle ear cholesteatoma as well as in papilloma. in the present study, polymerase chain reaction and in situ hybridization with human papillomavirus (hpv)-6 and -11 dna probes were used to detect the presence of hpv dna in 32 human middle ear cholesteatomas. only one specimen contained hpv-6 dna. although its occurrence may have been coincidental, it is also possible that the hyperproliferative ep ... | 2000 | 10839482 |
| solitary squamous cell papilloma of the lung in a 40-year-old woman with recurrent laryngeal papillomatosis. | a rare case of recurrent respiratory papillomatosis (rrp) is reported with a review of the literature. a 40-year-old japanese woman had suffered from rrp since 1 year of age. she developed a pulmonary squamous papilloma with a thin-walled cavity, which was suspected as being lung carcinoma. the trachea and bronchi around the tumor were intact, and no malignant transformation was present. two types of human papillomavirus, 6 and 16, were detected, both in the laryngeal and pulmonary papillomas by ... | 2000 | 10849335 |
| [papillomavirus infection in healthy women from st.petersburg]. | the study was aimed to evaluate the features of human papillomavirus (hpv) infection in healthy female inhabitants of st. petersburg. 309 attendants of gynecological clinics were recruited for the screening. all participants were of reproductive age and were lacking the clinical or morphological symptoms of hpv-related diseases. pcr revealed hpv-specific sequences in 90 (29%) women. hpv type distribution was similar to the one in usa and europe. hpv-positivity did not correlate with age of femal ... | 2000 | 10853416 |
| immunomodulatory therapy in the management of viral infections in patients with hiv infection. | human immunodeficiency virus (hiv) causes disease by infecting lymphocytes and progressively destroying critical regulatory and effector cells of the immune system, leaving patients vulnerable to a number of bacterial, fungal, and viral infections. facial herpes (herpes simplex virus-1 [hsv-1]), genital herpes (hsv-2), herpes zoster (varicella zoster virus), oral hairy leukoplakia (epstein-barr virus), kaposi's sarcoma (hhv-8), molluscum contagiosum, condyloma acuminata (human papillomavirus [hp ... | 2000 | 10861104 |
| human papillomavirus infection and cervical carcinoma. | 2000 | 10863634 | |
| human papillomavirus type 16 e6 induces self-ubiquitination of the e6ap ubiquitin-protein ligase. | the e6 protein of the high-risk human papillomaviruses (hpvs) and the cellular ubiquitin-protein ligase e6ap form a complex which causes the ubiquitination and degradation of p53. we show here that hpv16 e6 promotes the ubiquitination and degradation of e6ap itself. the half-life of e6ap is shorter in hpv-positive cervical cancer cells than in hpv-negative cervical cancer cells, and e6ap is stabilized in hpv-positive cancer cells when expression of the viral oncoproteins is repressed. expression ... | 2000 | 10864652 |
| the human papillomavirus type 16 e7 oncogene is required for the productive stage of the viral life cycle. | the production of the human papillomavirus type 16 (hpv-16) is intimately tied to the differentiation of the host epithelium that it infects. infection occurs in the basal layer of the epithelium at a site of wounding, where the virus utilizes the host dna replication machinery to establish itself as a low-copy-number episome. the productive stage of the hpv-16 life cycle occurs in the postmitotic suprabasal layers of the epithelium, where the virus amplifies its dna to high copy number, synthes ... | 2000 | 10864676 |
| cutaneous and mucous infections from human papilloma virus: new therapeutical approach. | 2000 | 10876965 | |
| enhanced oncogenicity of human papillomavirus type 16 (hpv16) variants in japanese population. | to investigate whether hpv16 e6 variants carry an elevated risk for cervical cancer in japanese population, we investigated the e6 sequence variation in 40 cervical intraepithelial neoplasias (cins) i-iii and 43 invasive cervical cancers (iccs), all positive for hpv16. hpv16 e6 variants were frequently found in iccs than in cins (88 vs. 65%, p=0.01). the e6 d25e, a rare variant in western countries, was most frequently observed in icc (44%). cin i/ii lesions with hpv16 variants were less likely ... | 2000 | 10880765 |
| consistent high viral load of human papillomavirus 16 and risk of cervical carcinoma in situ: a nested case-control study. | persistent infection with certain types of human papillomavirus (hpv) is believed to be a prerequisite for the development of cervical neoplasia. persistence may depend on certain characteristics, such as viral load, which has so far been given little attention. we investigated the association between hpv 16 viral load and cervical carcinoma in situ. | 2000 | 10881892 |
| efficacy in treatment of subclinical cervical hpv infection without intraepithelial neoplasia: systematic review. | the treatment of the subclinical human papillomavirus (hpv) infection of the uterine cervix is controversial. | 2000 | 10887387 |
| human papillomavirus types 16 e6 and e7 contribute differently to carcinogenesis. | high-risk human papillomaviruses (hpvs) are etiologically implicated in human cervical cancer. two viral genes, e6 and e7, are commonly found expressed in these cancer cells. we have previously shown that mice transgenic for the hpv-16 e6 gene or e7 gene, in which the e6 or e7 was expressed in the basal layer of epithelia, developed skin tumors. the spectrum of tumors derived from e6 and e7 mice differed, however; although most tumors derived from the e7-transgenic mice were benign, the majority ... | 2000 | 10662610 |
| prevalence of and risks for cervical human papillomavirus infection and squamous intraepithelial lesions in adolescent girls: impact of infection with human immunodeficiency virus. | data suggest that in adults, human papillomavirus (hpv) infections and their sequalae, squamous intraepithelial lesions (sils), occur more commonly among human immunodeficiency (hiv)-infected women because of the hiv-associated cd4+ t-cell immunosuppression. since adolescents are more likely to be early in the course of hiv and hpv infections, the study of both infections in this age group may help elucidate their initial relationship. | 2000 | 10665598 |
| use of fluorogenic histocompatibility leukocyte antigen-a*0201/hpv 16 e7 peptide complexes to isolate rare human cytotoxic t-lymphocyte-recognizing endogenous human papillomavirus antigens. | cervical cancer (cacx) is the second most common female malignancy worldwide and remains a clinical problem despite improvements in early detection and therapy. cacx and preinvasive cervical intraepithelial neoplasia (cin3) are strongly associated with infection by human papillomavirus (hpv), particularly types 16 and 18. two nonstructural viral proteins, e6 and e7, are constitutively expressed in cervical tumors and are crucial for the maintenance of the transformed phenotype. these proteins th ... | 2000 | 10667589 |
| purification of his-tagged proteins by immobilized chelate affinity chromatography: the benefits from the use of organic solvent. | recombinant proteins overexpressed in and purified from escherichia coli contain impurities that are toxic in biological assays. the application of affinity purification procedures is often not sufficient to remove these toxic components. we here describe a simple and fast, one-step protocol to remove these impurities highly efficiently. four recombinant proteins were overexpressed in e. coli as his-tagged fusion proteins and purified by immobilized metal chelate affinity chromatography on ni-nt ... | 2000 | 10648174 |
| antibodies against oncoproteins e6 and e7 of human papillomavirus types 16 and 18 in cervical-carcinoma patients from russia. | certain human papillomaviruses (hpv), mainly types 16 and 18, have been widely recognized as an essential etiologic factor for the development of carcinoma of the uterine cervix. the early hpv proteins e6 and e7 are consistently expressed in the tumor cells, and cervical-carcinoma patients can develop antibodies against these oncoproteins. for cervical-carcinoma patients from eastern europe and russia, detailed information on hpv dna prevalence and hpv-specific immune responses is limited. the p ... | 2000 | 10652419 |
| a novel and rapid pcr-based method for genotyping human papillomaviruses in clinical samples. | many human papillomavirus (hpv) genotypes are associated with cervical carcinoma. we demonstrate the utility of an innovative technique for genotyping of hpv in cervical tissue samples. this method provides an accurate means of identification of the specific hpv genotypes present in clinical specimens. by using the my09-my11 and the gp5(+)-gp6(+) consensus primer pairs, hpv sequences were amplified by nested pcr from dna isolated from cervical smear samples. this led to the production of an appr ... | 2000 | 10655368 |
| p53 alterations in recurrent squamous cell cancer of the head and neck refractory to radiotherapy. | the aim of the study was to determine the incidence of p53 alterations by mutation, deletion or inactivation by mdm2 or human papillomavirus (hpv) infection in recurrent squamous cell cancer of the head and neck (scchn) refractory to radiotherapy. twenty-two tumours were studied. the p53 status of each tumour was analysed by sequencing of exons 4-9 and by immunohistochemistry. mdm2 expression was assessed by immunohistochemistry and hpv infection was assessed by polymerase chain reaction of tumo ... | 2000 | 10646894 |
| induction of cell-cycle arrest in cervical cancer cells by the human immunodeficiency virus type 1 viral protein r. | to determine the ability of the human immunodeficiency virus type 1 (hiv-1) gene vpr to induce cell-cycle arrest in cervical cancer cells with or without human papillomavirus (hpv) type 16 e6 or e7 expression. | 2000 | 10636517 |
| recent striking changes in histological differentiation and rate of human papillomavirus infection in squamous cell carcinoma of the lung in okinawa, a subtropical island in southern japan. | the incidence of lung cancer in okinawa has been the highest in japan since 1975, and squamous cell carcinoma (scc), especially the well differentiated form, is the most prevalent form in okinawa, although well differentiated scc is relatively rare in mainland japan. furthermore, a high proportion of scc of the lung in okinawa was positive for human papillomavirus (hpv). in this study, we report recent striking changes in histological features and in the incidence of hpv infection. | 2000 | 11041057 |
| hybrid capture based human papillomavirus typing in cervical screening compared to cytology and histology. | cervical cancer is frequently associated with infection from various types of human papillomavirus (hpv) with high a oncogenic potential (high-risk types). commercial systems for hpv typing are available, but the question as to when hpv typing should be performed has not yet been solved. | 2000 | 11042905 |
| p53 codon 72 polymorphism in vulval cancer and vulval intraepithelial neoplasia. | p53 codon 72 polymorphism was analysed in uk women with human papillomavirus (hpv)-associated vulval intraepithelial neoplasia and vulval squamous cell carcinoma. arginine homozygotes were significantly less common in either group compared with controls. we conclude that the arginine polymorphism may confer protection against the development of hpv-associated vulval neoplasia. | 2000 | 11044351 |
| [vulvar lesions caused by hpv]. | human papillomavirus subclinical lesions are well known on the cervix and are identified by colposcopy after acetic acid staining. the transfer of this technique from the cervix to the vulva has led to the identification of areas of white epithelial changes which have been defined by similarity as vulvar subclinical hpv (vshpv) lesions. a critical revision of the different clinical vshpv lesions classifications, the vulvar diagnostic role of acetic acid staining, the natural history of hpv infec ... | 2000 | 11048476 |
| prevalence of human papillomavirus infection and its correlation with cervical lesions in commercial-sex workers in japan. | to investigate the prevalence of the human papillomavirus (hpv) infection and its correlation with cervical lesions in commercial-sex workers (csws) who attended a sexually transmitted disease (std) clinic in an entertainment area in tokyo. | 2000 | 11049234 |
| risk factors for cervical intraepithelial neoplasia in southwestern american indian women. | the authors assessed risk factors for cervical intraepithelial neoplasia (cin) among southwestern american indian women using case-control methods. cases were new mexico american indian women with biopsy-proven grade i (n = 190), grade ii (n = 70), or grade iii (n = 42) cervical lesions diagnosed between november 1994 and october 1997. controls were american indian women from the same indian health service clinics with normal cervical epithelium (n = 326). all subjects underwent interviews and l ... | 2000 | 11052549 |
| na+/h+ exchanger-dependent intracellular alkalinization is an early event in malignant transformation and plays an essential role in the development of subsequent transformation-associated phenotypes. | in this study we investigate the mechanism of intracellular ph change and its role in malignant transformation using the e7 oncogene of human papillomavirus type 16 (hpv16). infecting nih3t3 cells with recombinant retroviruses expressing the hpv16 e7 or a transformation deficient mutant we show that alkalinization is transformation specific. in nih3t3 cells in which transformation can be turned on and followed by induction of the hpv16 e7 oncogene expression, we demonstrate that cytoplasmic alka ... | 2000 | 11053239 |
| oral focal epithelial hyperplasia in a howler monkey (alouatta fusca). | oral focal epithelial hyperplasia is a rare and seldom reported disease in animals and humans induced by a papillomavirus. the present report is the first description of this disease in a neotropical primate, a howler monkey (alouatta fusca). the diagnosis was based on gross and microscopic findings. the generic papillomavirus antigen was identified by immunohistochemistry and was found not to be related to any human papillomavirus dna tested by in situ hybridization. this virus is probably a sp ... | 2000 | 11055880 |
| human papillomaviruses and dna ploidy in anal condylomata acuminata. | previous studies have emphasized the usefulness of dna ploidy measurement and human papillomavirus (hpv) detection as prognostic markers in low grade cervical lesions. we addressed the eventual relationship between hpv type, dna profile, and p53 tumor suppressor protein expression in anal condylomata acuminata to eventually determine parameters which may be considered as predictive risk factors for the development of cancer. dna ploidy was assessed by image cytometry after feulgen staining of co ... | 2000 | 10668198 |
| restored expression of fragile histidine triad protein and tumorigenicity of cervical carcinoma cells. | allelic losses in the short arm of chromosome 3 are common in cervical carcinomas. the fragile histidine triad (fhit) gene at chromosome region 3p14.2 is a candidate tumor suppressor gene that may play a role in cervical tumorigenesis. we and others have identified aberrant fhit transcripts and frequent loss of fhit protein expression in primary cervical cancers and high-grade noninvasive lesions but not in normal cervical tissues. the altered expression of fhit may be due to somatic mutations o ... | 2000 | 10675384 |
| both hpv and carcinogen contribute to the development of resistance to apoptosis during oral carcinogenesis. | oral carcinomas frequently contain human papilloma virus (hpv)-16/18. as p53 is degraded through interaction with hpv-16/18 products (e6/e7), p53 dysfunction may contribute to oral carcinogenesis. furthermore, epidemiological studies suggest that smoking history may be critical for oral carcinogenesis. to delineate the involvement of hpv-16 infection and carcinogen in oral carcinogenesis, park et al have established a multistep oral carcinogenesis model. overexpression of p53 altered the express ... | 2000 | 10675494 |
| determinants of low-risk and high-risk cervical human papillomavirus infections in montreal university students. | previous studies have been inconsistent about the degree of sexual transmissibility of cervical human papillomavirus (hpv) infection. the authors hypothesize that risk factors for hpv infection vary according to hpv type. | 2000 | 10676974 |
| asymptomatic penile hpv infection: a prospective study. | the occurrence of human papillomavirus (hpv) among males was analysed with the polymerase chain reaction (pcr) method. penile brush samples were taken once from 147 males attending for a control or for hpv non-related reasons, and consecutive samples were collected from 88 males re-attending the clinic. of the males attending once, 13% (19/147) were hpv dna positive and among the re-attenders 14% (12/88) were initially positive as compared with 33% (29/88) who were positive at least at one visit ... | 2000 | 10678474 |
| nuclear matrix attachment regions of human papillomavirus type 16 repress or activate the e6 promoter, depending on the physical state of the viral dna. | two nuclear matrix attachment regions (mars) bracket a 550-bp segment of the long control region (lcr) containing the epithelial cell-specific enhancer and the e6 promoter of human papillomavirus type 16 (hpv-16). one of these mars is located in the 5' third of the lcr (5'-lcr-mar); the other lies within the e6 gene (e6-mar). to study their function, we linked these mars in various natural or artificial permutations to a chimeric gene consisting of the hpv-16 enhancer-promoter segment and a repo ... | 2000 | 10684263 |
| recombinant adeno-associated virus expressing human papillomavirus type 16 e7 peptide dna fused with heat shock protein dna as a potential vaccine for cervical cancer. | in this study, we explore a potential vaccine for human papillomavirus (hpv)-induced tumors, using heat shock protein as an adjuvant, a peptide vaccine for safety, and adeno-associated virus (aav) as a gene delivery vector. the tumor vaccine was devised by constructing a chimeric gene which contained hpv type 16 e7 cytotoxic t-lymphocyte (ctl) epitope dna (m. c. feltkamp, h. l. smits, m. p. vierboom, r. p. minnaar, b. m. de jongh, j. w. drijfhout, j. ter schegget, c. j. melief, and w. m. kast, e ... | 2000 | 10684306 |
| hela cells are phenotypically limiting in cyclin e/cdk2 for efficient human papillomavirus dna replication. | human papillomaviral (hpv) origin-containing plasmids replicate efficiently in human 293 cells or cell extracts in the presence of hpv origin-recognition protein e2 and replication initiation protein e1, whereas cervical carcinoma-derived, hpv-18-positive hela cells or cell extracts support hpv dna replication poorly. we recently showed that hpv-11 e1 interacts with cyclin/cyclin-dependent kinase (cdk) complexes through an rxl motif and is a substrate for these kinases. e1 mutations in this moti ... | 2000 | 10692408 |
| interleukin 1 receptor antagonist gene polymorphism in women with vulvar vestibulitis. | vulvar vestibulitis is a chronic inflammatory syndrome of unknown cause and pathogenesis. we examined the relation between vulvar vestibulitis and polymorphisms in the gene coding for the interleukin 1 receptor antagonist, a naturally occurring down-regulator of proinflammatory immune responses. | 2000 | 10694325 |
| value of human papillomavirus testing. | 2000 | 10694358 | |
| the role of human papillomavirus in gynecological diseases. | papanicolaou (pap) smears of the cervix and biopsies of the cervix, vagina, vulva, and penis are a substantial portion of the work load of any anatomic pathology laboratory. the importance of samples from these sites is highlighted by the fact that their most common disease is dysplasia, which is sexually transmitted and a recognized risk factor for carcinoma. human papillomavirus (hpv) is an essential co-factor for the development of lower genital tract dysplasia. molecular testing for hpv has ... | 2000 | 10894184 |
| the restricted cellular host range of human herpesvirus 8. | a selection of primary and transformed cell types were evaluated for their susceptibility to infection with human herpesvirus 8 (hhv-8)/kaposi's sarcoma-associated herpesvirus. | 2000 | 10894276 |
| anal verrucous carcinoma and penile condylomata acuminata. | a 50-year-old man presented with recurrent tumorous lesions on the penis and the anal region. the anal lesion was histologically diagnosed as verrucous carcinoma (vc) and the penile lesions were in line with condylomata acuminata. samples taken from tumors of both sites were human papillomavirus (hpv) dna positive. two of them taken from the penis and the perianal region scored hpv dna 6 positive by using polymerase chain reaction and the southern blot method. treatment of both vc and condylomat ... | 2000 | 10894964 |
| variants of the long control region of human papillomavirus type 16. | expression of the human papillomavirus (hpv) e6 and e7 oncogenes is regulated on the transcriptional level by specific protein-binding sites contained in the viral long control region (lcr). sequence changes within the lcr region may have an impact on the transcription of viral oncogenes, possibly resulting in differences in the oncogenic potential of the virus. the present study was designed to determine the sequence variability of the lcr of hpv 16 and to assess whether certain lcr variants do ... | 2000 | 10899654 |
| chromium(vi) induces p53-dependent apoptosis in diploid human lung and mouse dermal fibroblasts. | some forms of hexavalent chromium [cr(vi)] are known to cause damage to respiratory-tract tissue and dna and are thought to be human lung carcinogens. in general, cr(vi) is mutagenic and carcinogenic at doses that also evoke some cell death, and we previously showed that the predominant mode of death is apoptosis. because p53 has been shown to initiate apoptosis after genotoxic insults, the objective of these experiments was to determine whether p53 is activated in and necessary for apoptosis of ... | 2000 | 10900468 |
| leaky scanning is the predominant mechanism for translation of human papillomavirus type 16 e7 oncoprotein from e6/e7 bicistronic mrna. | human papillomaviruses (hpv) are unique in that they generate mrnas that apparently can express multiple proteins from tandemly arranged open reading frames. the mechanisms by which this is achieved are uncertain and are at odds with the basic predictions of the scanning model for translation initiation. we investigated the unorthodox mechanism by which the e6 and e7 oncoproteins from human papillomavirus type 16 (hpv-16) can be translated from a single, bicistronic mrna. the short e6 5' untrans ... | 2000 | 10906182 |
| identification of domains of the human papillomavirus type 11 e1 helicase involved in oligomerization and binding to the viral origin. | the e1 helicase of papillomavirus is required, in addition to host cell dna replication factors, during the initiation and elongation phases of viral episome replication. during initiation, the viral e2 protein promotes the assembly of enzymatically active multimeric e1 complexes at the viral origin of dna replication. in this study we used the two-hybrid system and chemical cross-linking to demonstrate that human papillomavirus type 11 (hpv11) e1 can self-associate in yeast and form hexamers in ... | 2000 | 10906188 |
| papillary squamous cell carcinoma of the uterine cervix: report of three cases and a review of its classification. | papillary squamous cell carcinoma (scc) of the uterine cervix has been defined as a malignant squamous cell lesion characterized by a papillary architecture with fibrovascular cores and moderate to severe dysplasia devoid of frank keratinization and koilocytic change. papillary scc should be histopathologically delineated from other rare variants of scc with papillary features including verrucous and condylomatous carcinoma and the recently recognized (squamo-)transitional cell carcinoma of the ... | 2000 | 10907171 |
| persistence of human papillomavirus type 16 infection is associated with lack of cytotoxic t lymphocyte response to the e6 antigens. | our cross-sectional study suggested that cytotoxic t lymphocyte (ctl) responses have a protective effect in squamous intraepithelial lesion (sil) development. more ctl responses in women with human papillomavirus type 16 (hpv 16) infection without sils than with sils were detected. in the current longitudinal study, the role of ctl in clearing hpv 16 infection in women without sils was investigated. women with hpv 16 infection (n=51) were enrolled, along with hpv 16-negative control women (n=3). ... | 2000 | 10915094 |
| case-control study of plasma folate, homocysteine, vitamin b(12), and cysteine as markers of cervical dysplasia. | an association between b-complex vitamins and related compounds with the development of cervical neoplasia is biologically plausible, yet to the authors' knowledge epidemiologic investigations of these potential biomarkers are limited. | 2000 | 10918169 |
| p53 polymorphism in codon 72 and risk of human papillomavirus-induced cervical cancer: effect of inter-laboratory variation. | an association between codon-72 p53 polymorphism and risk of human papillomavirus (hpv)-induced cervical cancer has been found recently, but it has been difficult to replicate. in this study, we assess the impact of inter-laboratory variation in p53 genotyping on the validity of the proposed association. dna specimens were randomly selected from 54 invasive, squamous cell carcinoma cases, 52 hpv-negative, and 39 hpv-positive controls from a previous case-control study in brazil. codon-72 polymor ... | 2000 | 10918193 |
| cost-effectiveness of the conventional papanicolaou test with a new adjunct to cytological screening for squamous cell carcinoma of the uterine cervix and its precursors. | to estimate costs and outcomes of conventional annual papanicolaou (pap) test screening compared with biennial pap test plus speculoscopy (pps) screening for cervical neoplasms. | 2000 | 10927709 |
| modulation of the epidermal growth factor receptor by the human papillomavirus type 16 e5 protein in raft cultures of human keratinocytes. | it has been shown that the e5 protein of the human papillomavirus type 16 modulates epidermal growth factor receptor downregulation in monolayer cultures of human keratinocytes and mouse fibroblasts. we have now analysed the effect of this protein on the expression, the distribution and the activation of egf receptors in raft cultures derived from an e5-transfected human keratinocyte cell line. the epithelia generated in these cultures were stratified and exhibited suprabasal expression of cytok ... | 2000 | 10928456 |
| human papillomavirus in venous ulcers with and without squamous cell carcinoma. | malignant transformation of chronic wounds is a well-known, albeit rare, phenomenon. we examined archival paraffin blocks of samples of squamous cell carcinoma (scc) in chronic venous leg ulcers previously taken from 23 patients and of chronic noncancerous venous leg ulcers from 35 patients for the presence of human papillomavirus (hpv) dna. the methods used were the polymerase chain reaction (pcr) with gp05+/06+ (mucosal) and nested pcr with cp65/70 and cp66/69 (ev-associated) primers. a subseq ... | 2000 | 10929767 |
| inhibition of human papillomavirus type 16 gene expression by nordihydroguaiaretic acid plant lignan derivatives. | several methylated derivatives of a plant lignan, nordihydroguaiaretic acid (ndga) were found to be potent anti-viral agents by suppressing sp1 regulated transcription within the sexually transmitted viruses human immunodeficiency virus (hiv) and herpes simplex virus (hsv). a prominent sp1 dna binding site within many human papillomavirus (hpv) promoters has been noted to play an active role in hpv gene expression. in this report it is shown that the three ndga derivatives, mal.4, m(4)n, and tet ... | 2000 | 10930643 |
| human papillomavirus types 56 and 59 in novel cervical cancer cell lines. | 2000 | 10937347 | |
| differential behavior of vegf receptor expression and response to tnp-470 in two immortalized human endothelial cell lines. | angiogenesis consists of endothelial cell proliferation, migration and tube formation. it is useful to investigate endothelial cell behavior using immortalized endothelial cell lines. we characterized cell growth property, growth factor dependency and response to angioinhibitory drugs; tnp-470, staurosporine, radicicol and genistein, using human umbilical vein endothelial cells (huvecs) immortalized by human papilloma virus (hpv)-16 e6-e7, named huvecs/e6-e7, and huvecs/e6-e7 transformed by v-ki ... | 2000 | 10938394 |
| identification in humans of hpv-16 e6 and e7 protein epitopes recognized by cytolytic t lymphocytes in association with hla-b18 and determination of the hla-b18-specific binding motif. | human papilloma virus type 16 (hpv-16) is the hpv most frequently associated with cervical carcinoma in humans. for the prevention or treatment of cervical carcinoma, the e6 and e7 oncoproteins appear to be good targets for vaccine-induced cytotoxic t lymphocytes (ctl). lipopeptide vaccination is an efficient way of stimulating cellular responses. however, to synthesize effective lipopeptides, it is necessary to define which epitopes are immunogenic. in this study we first determined that peptid ... | 2000 | 10940919 |
| sexually transmitted infection and teenage sexuality. | the twin epidemics of sexually transmitted infection and nonmarital pregnancy and their consequences are two of the most significant issues faced by americans today. approximately 900,000 teenagers become pregnant each year. research indicates that these pregnancies often limit future educational and economic opportunities for mother, father, and child. sexually transmitted infections also pose a major threat to adolescents. for example, during a 3-year study of sexually active women at a major ... | 2000 | 10942466 |
| the human papillomavirus type 16 e6 and e7 oncoproteins cooperate to induce mitotic defects and genomic instability by uncoupling centrosome duplication from the cell division cycle. | loss of genomic integrity is a defining feature of many human malignancies, including human papillomavirus (hpv)-associated preinvasive and invasive genital squamous lesions. here we show that aberrant mitotic spindle pole formation caused by abnormal centrosome numbers represents an important mechanism in accounting for numeric chromosomal alterations in hpv-associated carcinogenesis. similar to what we found in histopathological specimens, hpv-16 e6 and e7 oncoproteins cooperate to induce abno ... | 2000 | 10944189 |
| association between activated k-ras and c-erbb-2 oncogenes with "high-risk" and "low-risk" human papilloma virus types in preinvasive cervical lesions. | clinical and epidemiological data have linked cervical cancer to the human papilloma virus (hpv) infection. however, the presence of hpv infection alone is not enough to cause tumorigenesis, suggesting a role for additional host-cell genetic factors. the aim of the present work was to study the association of k-ras and c-erbb-2 mutations in cervical tissue samples with different grades of dysplasia and infected with hpv-6 ("low-risk" type) or hpv-16 and hpv-18 ("high-risk" types). negative hpv-d ... | 2000 | 10946249 |
| penile hybrid verrucous-squamous carcinoma associated with a superficial inguinal lymph node metastasis. | a 62-year-old japanese man who presented with penile carcinoma is reported. the initial exophytic neoplasm excised from the coronal sulcus and prepuce on the abdominal side of the penis was diagnosed histologically as verrucous carcinoma. twenty-six months after the primary operation, an epithelial neoplasm recurred within the scar of the primary operation. the neoplasm histologically showed verrucous carcinoma and multiple invasive foci of conventional squamous cell carcinoma in the advancing e ... | 2000 | 10949460 |
| anal squamous intraepithelial lesions in human immunodeficiency virus-positive men and women. | studies from the era prior to the introduction of highly active antiretroviral therapy (haart) have shown that the prevalence of anal human papillomavirus (hpv) infection and anal squamous intraepithelial lesions (asil) was very high among human immunodeficiency virus (hiv)-positive homosexual men, and to a lesser extent, among hiv-negative homosexual men. prospective data also show that the incidence of high-grade asil (hsil), the putative invasive cancer precursor lesion, was high among both h ... | 2000 | 10950374 |
| erythroplasia of queyrat: coinfection with cutaneous carcinogenic human papillomavirus type 8 and genital papillomaviruses in a carcinoma in situ. | erythroplasia of queyrat is a carcinoma in situ that mainly occurs on the glans penis, the prepuce, or the urethral meatus of elderly males. up to 30% progress to squamous cell carcinoma. the cause of erythroplasia of queyrat is largely unknown. human papillomavirus type 16 dna has previously been detected only in very few distinctly characterized patients. we have investigated 12 paraffin-embedded biopsies from eight patients with penile erythroplasia of queyrat and control biopsies of inflamma ... | 2000 | 10951274 |
| connexin 43 expression is downregulated in raft cultures of human keratinocytes expressing the human papillomavirus type 16 e5 protein. | a decrease in gap junction-mediated cell-to-cell communication has previously been observed in monolayer cultures of human keratinocytes (hacat cells) expressing the human papillomavirus type 16 e5 (hpv16 e5) gene and attributed to the reduced phosphorylation of connexin 43, the most abundant connexin in hacat cells. in line with this observation, we have now analyzed the effect of hpv16 e5 on connexin 43 expression in raft cultures produced by transfected hacat cells. these keratinocytes transc ... | 2000 | 10955727 |
| iga antibodies to the 27-kda heat-shock protein in the genital tracts of women with gynecologic cancers. | heat-shock proteins promote cell survival under adverse environmental conditions. synthesis of the 27-kda (hsp27), 70-kda (hsp70), and 90-kda (hsp90) heat-shock proteins is increased in malignantly transformed cells and has been associated with tumor proliferation, metastasis, and resistance to chemotherapeutic agents. the increased expression of heat-shock proteins and their association with tumor-specific antigens may result in local immunity to the heat-shock proteins. we examined the occurre ... | 2000 | 10956393 |
| detection of human papillomavirus in laryngeal squamous dysplasia and carcinoma. an in situ hybridization and signal amplification study. | we examined the presence of human papillomavirus (hpv) dna in 65 cases of laryngeal squamous dysplasia and carcinomas using in situ hybridization with signal amplification in paraffin sections. hybridization was performed with biotinylated dna probes for hpv 6/11, 16/18, 31/33 and wide-spectrum hpv (6, 11, 16, 30, 31, 45, 51 and 52). hpv dna was found in 7 cases of the total sample (10.7%); it was also found in 4 out of 45 (8.8%) cases of invasive carcinoma and in 5 out of 33 (15.5%) cases of sq ... | 2000 | 10958409 |