Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| increased milk levels of transforming growth factor-alpha, beta1, and beta2 during escherichia coli-induced mastitis. | among the gram-negative bacteria that cause mastitis, escherichia coli are the most prevalent. the innate immune system provides initial protection against e. coli infection by detecting the presence of the foreign pathogens and by mounting an inflammatory response, the latter of which is mediated by cytokines such as il-1beta, il-8, and tumor necrosis factor (tnf)-alpha. although changes in these cytokines during mastitis have been well-described, it is believed that other mediators moderate ma ... | 2005 | 15905428 |
| expression cloning of gamma interferon-inducing antigens of mycobacterium avium subsp. paratuberculosis. | three recombinant proteins, map10, map39, and map41, produced based on nucleotide sequences obtained from the screening of mycobacterium avium subsp. paratuberculosis genomic library expressed in escherichia coli significantly elicited gamma interferon production in peripheral blood mononuclear cells from infected cattle. two of these proteins were members of the ppe protein family. | 2005 | 15908411 |
| targeted and proximity-dependent promiscuous protein biotinylation by a mutant escherichia coli biotin protein ligase. | a method for general protein biotinylation by enzymatic means has been developed. a mutant form (r118g) of the biotin protein ligase (bira) of escherichia coli is used and biotinylation is thought to proceed by chemical acylation of protein lysine side chains by biotinoyl-5'-amp released from the mutant protein. bovine serum albumin, chloramphenicol acetyltransferase, immunoglobulin chains and rnase a as well as a large number of e. coli proteins have been biotinylated. the biotinylation reactio ... | 2005 | 15992681 |
| [field study of the prevalence and diagnosis of diarrhea-causing agents in the newborn calf in a swiss veterinary practice area]. | the prevalence of cryptosporidia, rotavirus, bovine coronavirus and escherichia coli f5 (k99) in dairy calves with diarrhea and in healthy calves was established in a limited area served by a veterinary practice. immuno-chromatographic rapid tests (fastest strips) were applied in the field and their results were compared to the ones obtained with standard methods (modified ziehl-neelsen stain, antigen-elisa and cultivation). in 78% of the calves with diarrhea (n=46) and in 29% of the healthy cal ... | 2005 | 15999634 |
| characterization of a shiga toxin-, intimin-, and enterotoxin hemolysin-producing escherichia coli ont:h25 strain commonly isolated from healthy cattle. | among bovine fecal and recto-anal mucosal swab samples cultured in our laboratory for escherichia coli o157:h7, we frequently isolated e. coli organisms that were phenotypically similar to the o157:h7 serotype as non-sorbitol fermenting and negative for beta-glucuronidase activity but serotyped o nontypeable:h25 (ont:h25). this study determined the prevalence and virulence properties of the e. coli ont:h25 isolates. among dairy and feedlot cattle (n = 170) sampled in washington, idaho, and alber ... | 2005 | 16000438 |
| characterization and functional study of antrodia camphorata lipopolysaccharide. | lipopolysaccharide (lps) is a highly proinflammatory molecule isolated from bacteria. this study demonstrated the existence of lps in a medicinal fungus, antrodia camphorata. because no lps had been identified in any fungus organism, the purification of lps from a. camphorata was attempted. lpss from six strains of a. camphorata (35396, 35398, 35716, b71, b85, and b86) were isolated. chemical and functional properties were investigated on the fungus lps. compositional analysis revealed that sorb ... | 2005 | 15656690 |
| escherichia coli o157:h7 infections associated with ground beef from a u.s. military installation--okinawa, japan, february 2004. | in february 2004, the okinawa prefectural chubu health center (ochc) and the okinawa prefectural institute of health and environment (oihe), japan, investigated three cases of escherichia coli o157:h7 infection in a japanese family associated with eating ground beef. public health officials from multiple agencies in japan and the united states collaborated on this investigation, which resulted in a voluntary recall of approximately 90,000 pounds of frozen ground beef in the united states and at ... | 2005 | 15660018 |
| kinetics of local and systemic isoforms of serum amyloid a in bovine mastitic milk. | the aim of the present study was to characterise the serum amyloid a (saa) response to intramammary inoculation of escherichia coli and to examine the distribution of hepatically and extrahepatically produced saa isoforms in plasma and milk from cows with mastitis. milk and plasma saa concentrations were determined before and after experimental induction of e. coli mastitis in six dairy cows. the milk saa response was characterised by low or undetectable levels before inoculation, very rapid and ... | 2005 | 15661328 |
| immunoreactivity of the mycobacterium avium subsp. paratuberculosis 19-kda lipoprotein. | the mycobacterium tuberculosis 19-kda lipoprotein has been reported to stimulate both t and b cell responses as well as induce a number of th1 cytokines. in order to evaluate the mycobacterium avium subsp. paratuberculosis (m. avium subsp. paratuberculosis) 19-kda lipoprotein as an immunomodulator in cattle with johne's disease, the gene encoding the 19-kda protein (map0261c) was analyzed. | 2005 | 15663791 |
| escherichia coli o157:h7 vaccine field trial in 9 feedlots in alberta and saskatchewan. | a feedlot trial was conducted to assess the efficacy of an escherichia coli o157:h7 vaccine in reducing fecal shedding of e. coli o157:h7 in 218 pens of feedlot cattle in 9 feedlots in alberta and saskatchewan. pens of cattle were vaccinated once at arrival processing and again at reimplanting with either the e. coli o157:h7 vaccine or a placebo. the e. coli o157:h7 vaccine included 50 microg of type iii secreted proteins. fecal samples were collected from 30 fresh manure patties within each fee ... | 2005 | 16187717 |
| isolation, catalytic properties, and competitive inhibitors of the zinc-dependent murine glutaminyl cyclase. | murine glutaminyl cyclase (mqc) was identified in the insulinoma cell line beta-tc 3 by determination of enzymatic activity and rt-pcr. the cloned cdna was expressed in the secretory pathway of the methylotrophic yeast pichia pastoris and purified after fermentation using a new three-step protocol. mqc converted a set of various substrates with very similar specificity to human qc, indicating a virtually identical catalytic competence. furthermore, mqc was competitively inhibited by imidazole de ... | 2005 | 16201766 |
| effects of cattle feeding regimen and soil management type on the fate of escherichia coli o157:h7 and salmonella enterica serovar typhimurium in manure, manure-amended soil, and lettuce. | survival of the green fluorescent protein-transformed human pathogens escherichia coli o157:h7 and salmonella enterica serovar typhimurium was studied in a laboratory-simulated lettuce production chain. dairy cows were fed three different roughage types: high-digestible grass silage plus maize silage (6:4), low-digestible grass silage, and straw. each was adjusted with supplemental concentrates to high and low crude protein levels. the pathogens were added to manure, which was subsequently mixed ... | 2005 | 16204535 |
| comparison of rectoanal mucosal swab cultures and fecal cultures for determining prevalence of escherichia coli o157:h7 in feedlot cattle. | we compared fecal samples with samples collected with rectoanal mucosa swabs (rams) to determine the prevalence of escherichia coli o157 in feedlot cattle (n = 747). escherichia coli o157 was detected in 9.5% of samples collected with rams and 4.7% of samples tested by fecal culture. pulsed-field gel electrophoresis analysis of isolates suggested that the strains colonizing the rectoanal junction were the same as those from the feces. mucosal swab sampling was more sensitive than fecal sampling ... | 2005 | 16204574 |
| in-line milk filter analysis: escherichia coli o157 surveillance of milk production holdings. | escherichia coli o157 is a major etiological agent of food-borne illness. bovine animals are recognized reservoirs for this organism and represent a significant source from where these pathogens can enter the food chain. food products derived from these animals are convenient vehicles, and are often the focal point(s) of infection. as a useful strategy to provide herd-level surveillance and to investigate for the presence of this pathogen in a population of irish dairy cattle, milk filters from ... | 2005 | 16217925 |
| virulence genes in verocytotoxigenic escherichia coli strains isolated from humans and cattle. | verocytotoxigenic escherichia coli (vtec) causing diarrhoea, haemorrhagic colitis and haemolytic-uremic syndrome usually have additional traits such as the adhesin intimin and a large plasmid that seems to increase virulence. there are, however, isolates of vtec causing serious symptoms that do not harbour these traits. in the present study we have used pcr with primers detecting adhesin genes other than eaea, namely fima, papc, sfad/sfae and daae. we have also used pcr to detect the genes hlya ... | 2005 | 16218932 |
| escherichia coli o157:h7 in drinking water from private water supplies in the netherlands. | the microbiological quality of drinking water from 144 private water supplies in the netherlands was tested and additionally the occurrence of escherichia coli o157 was examined. faecal indicators were enumerated by using standard membrane filtration methods. the presence of e. coli o157 was determined using a specific enrichment method. eleven percent of the samples contained faecal indicators whereas e. coli o157:h7 was isolated from 2.7% of the samples that otherwise met the drinking water st ... | 2005 | 16219332 |
| effect of manure on escherichia coli attachment to soil. | attachment of bacteria to soil is an important component of bacterial fate and transport. escherichia coli are commonly used as indicators of fecal contamination in the environment. despite the fact that e. coli are derived exclusively from feces or manure, effect of the presence of manure colloids on bacteria attachment to agricultural soils was never directly studied. the objective of this work was to evaluate the magnitude of the effect of manure on e. coli attachment to soil. escherichia col ... | 2005 | 16221829 |
| decline of zoonotic agents in livestock waste and bedding heaps. | to measure the rates of decline of zoonotic agents introduced into heaps of spent bedding and faecal wastes generated by commercially farmed livestock and managed in a similar way to that of a working farm. | 2005 | 16033467 |
| gross abnormalities, bacteriology and histological lesions of uteri of dairy cows failing to conceive or maintain pregnancy. | to describe the gross pathology, bacteriology and histopathology of the reproductive tracts of dairy cows that failed to conceive or maintain pregnancy. | 2005 | 16044186 |
| effect of feeding live yeast products to calves with failure of passive transfer on performance and patterns of antibiotic resistance in fecal escherichia coli. | fifty-two newborn holstein calves with serum igg concentrations less than 0.73 g.dl(-1) were randomly assigned to one of four treatments: no added live yeast (control), 0.5 g of live yeast added to the grain for 84 d (sc; saccharomyces cerevisiae), 0.5 g of live yeast added to the milk for 42 d (sb; s. cerevisiae, spp. boulardii), and 0.5 g of live yeast added to the grain for 84 d and to the milk for 42 d (scsb). calves were offered 440 g of milk replacer dm for the first 42 d and grain for ad ... | 2005 | 16045891 |
| e. coli proteolytic activity in milk and casein breakdown. | previous studies have focused on both lps and e. coli experimental mastitis and underlined the respective roles of endogenous proteolysis (including plasmin from the blood stream and other proteases from milk leukocytes), as well as the presence of e. coli in a more intricate system. the aim of this study was to assess the role of e. coli in milk proteolysis and especially that of its proteases in casein breakdown. the first part consisted in the incubation of 104 cfu.ml(-1) of the e. coli strai ... | 2005 | 16045896 |
| characterization of two major groups of diarrheagenic escherichia coli o26 strains which are globally spread in human patients and domestic animals of different species. | twenty-three escherichia coli o26 strains from humans, cattle, sheep, pigs and chicken were investigated for virulence markers and for genetic similarity by pulsed field gel electrophoresis and multi locus sequence typing. two groups of genetically closely related o26 strains were defined. one group is formed by enteropathogenic (epec) and enterohemorrhagic (ehec) e. coli strains, which do not ferment rhamnose and dulcitol and most of these carry a plasmid encoding enterohemolysin. the other gro ... | 2005 | 16046083 |
| dairy farm investigation on shiga toxin-producing escherichia coli (stec) in kolkata, india with emphasis on molecular characterization. | an investigation was conducted to determine the distribution, virulence gene profile and phenotypes of shiga toxin-producing escherichia coli (stec) strains within a dairy farm in kolkata, india by characterizing the stec strains isolated from healthy dairy cow and calf stool samples, raw milk and farm floor swabs from july 2001 to march 2002. primary screening by multiplex-pcr detected stx1 and stx2, the common virulence genes of stec, in 18.9% of cow faeces, 32.4% of calf stool samples, 21.6% ... | 2005 | 16050506 |
| the role of hiti, a serine protease inhibitor from haematobia irritans irritans (diptera: muscidae) in the control of fly and bacterial proteases. | blood-sucking arthropods are vectors responsible for the transmission of several pathogens and parasites to vertebrate animals. the horn fly haematobia irritans irritans (diptera: muscidae) and the tick boophilus microplus are important hematophagous ectoparasites that cause losses in cattle production. a serine protease inhibitor from a thorax extract of the fly h. irritans irritans (hiti) was previously isolated, characterized and cloned. in the present study we described the expression, purif ... | 2005 | 16054488 |
| characterization and calculation of a cytochrome c-cytochrome b5 complex using nmr data. | to identify the binding site for bovine cytochrome b(5) (cyt b(5)) on horse cytochrome c (cyt c), cross-saturation transfer nmr experiments were performed with (2)h- and (15)n-enriched cyt c and unlabeled cyt b(5). in addition, chemical shift changes of the cyt c backbone amide and side chain methyl resonances were monitored as a function of cyt b(5) concentration. the chemical shift changes indicate that the complex is in fast exchange, and are consistent with a 1:1 stoichiometry. a k(a) of (4 ... | 2005 | 16060674 |
| vegi-192, a new isoform of tnfsf15, specifically eliminates tumor vascular endothelial cells and suppresses tumor growth. | we determined the antiangiogenic and anticancer activity of vegi-192, a new isoform of tnfsf15 (vegi, tl1), with a lewis lung cancer murine tumor model. | 2005 | 16061878 |
| microbial quality of runoff following land application of cattle manure and swine slurry. | concentrations of human health-related microorganisms in runoff from agricultural plots (0.75 m x 2 m) treated with fresh and aged cattle manure, swine slurry and no manure (control) were determined. three consecutive simulated rainfall events, producing 35 mm rainfall and separated by 24 h, were carried out for each plot. fecal indicator (escherichia coli, enterococci, clostridium perfringens and coliphage) loads released in rainfall runoff from plots treated with fresh cattle manure, aged catt ... | 2005 | 16075941 |
| escherichia coli o157 : h7 forms attaching and effacing lesions at the terminal rectum of cattle and colonization requires the lee4 operon. | enterohaemorrhagic escherichia coli o157 : h7 is a human pathogen that causes no apparent disease in cattle, its primary reservoir host. recent research has demonstrated that e. coli o157 : h7 predominately colonizes the distal few centimetres of the bovine rectum, and in this study, the lee4 operon encoding a type iii secretion system translocon and associated proteins was shown to be essential for colonization. a deletion mutant of lee4 failed to colonize cattle, in contrast to a co-inoculated ... | 2005 | 16079353 |
| characterization and enzymatic degradation of sup35nm, a yeast prion-like protein. | transmissible spongiform encephalopathies (tses) are believed to be caused by an unconventional infectious agent, the prion protein. the pathogenic and infectious form of prion protein, prpsc, is able to aggregate and form amyloid fibrils, very stable and resistant to most disinfecting processes and common proteases. under specific conditions, prpsc in bovine spongiform encephalopathy (bse) brain tissue was found degradable by a bacterial keratinase and some other proteases. since this disease-c ... | 2005 | 16081647 |
| host species-specific metabolic fingerprint database for enterococci and escherichia coli and its application to identify sources of fecal contamination in surface waters. | a metabolic fingerprint database of enterococci and escherichia coli from 10 host groups of animals was developed to trace the sources of fecal contamination in surface waters. in all, 526 biochemical phenotypes (bpts) of enterococci and 530 e. coli bpts were obtained from 4,057 enterococci and 3,728 e. coli isolates tested. of these, 231 enterococcus bpts and 257 e. coli bpts were found in multiple host groups. the remaining 295 enterococcus bpts and 273 e. coli bpts were unique to individual h ... | 2005 | 16085837 |
| mutagenesis of pepa suggests a new model for the xer/cer synaptic complex. | pepa is an aminopeptidase and also functions as a dna-binding protein in two unrelated systems in escherichia coli: xer site-specific recombination and transcriptional regulation of carab. in these systems, pepa binds to and brings together distant segments of dna to form interwrapped, nucleosome-like structures. here we report the selection of pepa mutants that were unable to support efficient xer recombination. these mutants were defective in dna-binding and in transcriptional regulation of ca ... | 2005 | 16091035 |
| evidence of transmission of verocytotoxin-producing escherichia coli o111 from a cattle stable to a child. | infections with verocytotoxigenic escherichia coli (vtec) other than o157 have been assumed to have the same epidemiology as those with vtec o157, but the source of infection is rarely defined for sporadic cases. this report describes a child with vtec o111:h- infection who was probably infected by playing in a cattle stable and/or by drinking raw milk from the cows in this stable. e. coli o111 isolates colonising the cattle were indistinguishable from the patient isolate by the use of serotypin ... | 2005 | 16104995 |
| e. coli o157 and salmonella spp. in white-tailed deer and livestock. | escherichia coli o157 and salmonella spp. are among the leading causes of food-borne illness in the united sates and bacteria have been isolated from numerous ruminant animal sources. the objective of this study was to assess the incidence of e. coli o157 and salmonella spp. in white-tailed deer (odocoileus virginianus) and livestock simultaneously grazing the same rangeland. escherichia coli o157 was found in 1.25% of cattle, 1.22% of sheep, and 5.00% of water all from samples taken in septembe ... | 2005 | 16107036 |
| isolation and characterization of shiga toxin-producing escherichia coli strains from raw milk cheeses in france. | to evaluate shiga toxin-producing eschericha coli (stec) prevalence in 1039 french raw milk cheeses including soft, hard, unripened and blue mould cheeses, and to characterize the stec strains isolated (virulence genes and serotypes). | 2005 | 16108913 |
| lymphocytes from one side of the bovine mammary gland migrate to the contra lateral gland and lymph node tissue. | the four quarters of bovine mammary glands are completely separated and two quarters on each side (right or left) are connected to ipsi lateral supra mammary lymph nodes. it is not clear whether cells infused into the cistern of the mammary gland are capable of migrating to lymph nodes or the general circulation. to examine cell migration, a prescapular lymph node was removed from each of two lactating and three non-lactating dairy cows, and isolated lymphocytes were stained with hoechst 33342. ... | 2005 | 16111770 |
| biotin carboxyl carrier protein co-purifies as a contaminant in core-streptavidin preparations. | we have successfully cloned and expressed core-streptavidin in escherichia coli. core-streptavidin was expressed in shaker flask culture as a soluble protein, isolated by periplasmic extraction, purified by immobilized metal affinity chromatography column, and analyzed for its size, thermal stability, and biotin-binding activity. in western blots using streptavidin-horseradish peroxidase (hrp) as a probe, we identified a contaminant that co-purified with core-streptavidin, identified as biotin c ... | 2005 | 16118413 |
| [drug sensitivity of vero toxin-producing escherichia coli o157:h7 (vtec o157:h7) isolated from human diarrhea and healthy cows]. | as a part of studies on the source of infection of vero toxin-producing escherichia coli (vtec), o157:h7 strains isolated from human infectious enteritis between 1986 and 1995 and o157:h7 strains isolated from feces of milk cows between 2001 and 2003 were subjected to drug sensitivity test with drugs widely used as therapeutic drugs for various infectious diseases in humans and animals, and the following results were obtained. 1) drug sensitivity tests with 20 drugs were performed in 52 strains ... | 2005 | 16119803 |
| short report: isolation of escherichia coli o157:h7 from fecal samples of cows in vietnam. | enterohemorrhagic escherichia coli o157:h7 was isolated for the first time in vietnam. shiga toxin-producing e. coli were isolated from 8 of 100 cows examined. the two strains showing serotype o157:h7 carried the eae, ehxa, and stx2c genes, but the other six were negative for the eae gene. | 2005 | 16172486 |
| [expression, purification and antigen activity analysis of the n-terminal domain of lipoprotein lppq of mycoplasma mycoide subsp. mycoides sc]. | the gene sequence coding the n-terminal domain of lppq was amplified from mycoplasma mycoides subsp. mycoides sc (mmmsc) hvri x strain by pcr using special primers and was cloned into the ecor i /sal i sites of pet32a vector to construct the expression recombinant plasmids. the recombinant plasmids were indentified by restriction digestion, pcr and sequence analysis. the gene was overexpressed in escherichia coli bl21 (de3) host cell and the soluble protein was purified with ni-nta his. bind pur ... | 2005 | 16342778 |
| mass screening for antimicrobial resistant escherichia coli in dairy cows in northern new south wales. | to describe aspects of the epidemiology of antimicrobial resistance in escherichia coli shed in the faeces of milking cows in a dairying region of new south wales. | 2005 | 16315669 |
| association of virulence genotype with phylogenetic background in comparison to different seropathotypes of shiga toxin-producing escherichia coli isolates. | the distribution of virulent factors (vfs) in 287 shiga toxin-producing escherichia coli (stec) strains that were classified according to karmali et al. into five seropathotypes (m. a. karmali, m. mascarenhas, s. shen, k. ziebell, s. johnson, r. reid-smith, j. isaac-renton, c. clark, k. rahn, and j. b. kaper, j. clin. microbiol. 41:4930-4940, 2003) was investigated. the associations of vfs with phylogenetic background were assessed among the strains in comparison with the different seropathotype ... | 2005 | 16333104 |
| numbers of fecal streptococci and escherichia coli in fresh and dry cattle, horse, and sheep manure. | livestock are known contributors to stream pollution. numbers of fecal streptococci and escherichia coli in manure naturally deposited by livestock in the field are needed for activities related to bacterial source tracking and determining maximum daily bacterial loading of streams. we measured populations of fecal streptococci and e. coli in fresh and dry manure from cattle (bos taurus l.), horses (equus caballus l.), and sheep (ovis aires l.) on farms in southern idaho. populations of indicato ... | 2005 | 16333344 |
| cloning, expression, purification, crystallization and preliminary crystallographic study of the protein module (biv2-helix) in the fusion core of bovine immunodeficiency-like virus (biv) gp40. | the fusion core of bovine immunodeficiency virus (biv) gp40 is proposed to be involved in membrane fusion. however, no crystal structures are yet available. a predicted protein module biv2-helix of bivgp40 has been expressed in escherichia coli and purified by chromatography. recombinant biv2-helix was crystallized using the hanging-drop vapour-diffusion technique at 291 k. the crystals were grown in mpd and belonged to the primitive rhombohedral space group r3, with unit-cell parameters a = 39. ... | 2005 | 16511008 |
| identification of escherichia coli o157:h7 meat processing indicators for fresh meat through comparison of the effects of selected antimicrobial interventions. | fresh meat products can become contaminated with the pathogen escherichia coli o157:h7 during the slaughter process; therefore, an e. coli o157:h7 indicator to verify the effectiveness of process controls in slaughter establishments would be extremely useful. the hides of 20 beef cattle were sampled, and 113 bacterial isolates were obtained. thirteen of these isolates representing four genera, escherichia, enterobacter, providencia, and serratia, were selected based on growth and biochemical cha ... | 2005 | 16355829 |
| assessment of accuracy of disk diffusion tests for the determination of antimicrobial susceptibility of common bovine mastitis pathogens: a novel approach. | a novel approach was used to assess disk diffusion accuracy for determination of antibiotic susceptibility of various bovine mastitis pathogens (escherichia coli, staphylococcus aureus, staphylococcus chromogenes, and streptococcus dysgalactiae). mic and disk diffusion diameters were compared for 587 bovine mastitis bacterial isolates collected in israel and 3,186 drug-organism combinations. results were analyzed by roc curves, bayesian statistics, and standard descriptive methods. low correlati ... | 2005 | 16359193 |
| a comparison of the antimicrobial activity of garlic, ginger, carrot, and turmeric pastes against escherichia coli o157:h7 in laboratory buffer and ground beef. | the antimicrobial effects of garlic, ginger, carrot and turmeric pastes against escherichia coli o157:h7 in laboratory buffer and model food system were investigated. turmeric paste, fresh carrot, ginger and garlic pastes from roots, and commercial ginger and garlic paste were heated alone or with buffered peptone water (bpw) or ground beef at 70 degrees c for 7 min. all samples were inoculated with a three strain cocktail of overnight cultures of e. coli o157: h7 and stored at 4 degrees c and 8 ... | 2005 | 16366855 |
| characterization of escherichia coli and salmonella enterica from cattle feed ingredients. | the objectives of this study were (1) to evaluate the frequency with which feed ingredients or mixed feeds in cattle feedlots were contaminated with escherichia coli and salmonella spp. and (2) to evaluate the antimicrobial susceptibility patterns of non-type-specific escherichia coli and salmonella spp. recovered from feed ingredients or mixed feeds. approximately 30 individual samples were collected from each of several feed commodities present on two cattle feedlots each month for 1 year. hal ... | 2005 | 16366856 |
| antimicrobial resistance and class 1 integrons in pathogenic escherichia coli from dairy farms. | the goal of this study was to assess the prevalence of antimicrobial resistance and class 1 integrons, including integron-associated genes, in 24 escherichia coli isolates from dairy farms. escherichia coli isolates (n = 14) from dairy cows with mastitis (ecdm), shiga toxin-producing (stec) o157:h7 from cull dairy cow fecal samples (n = 9) and bulk tank milk (n = 1) were evaluated for sensitivity to 19 antimicrobial agents used commonly in human and/or veterinary medicine. multiplex pcr was used ... | 2005 | 16366857 |
| identification of putative adhesin genes in shigatoxigenic escherichia coli isolated from different sources. | shiga toxin-producing escherichia coli (stec) is an important pathogen responsible for severe human intestinal and systemic infections. the bacterial factors required for colonization of the hosts are still not well defined. in this study, the prevalence of seven putative adhesive genes that are not encoded in the locus of enterocyte effacement (lee) in 74 stec strains isolated from humans (n=39), food (n=6), cattle (n=11), and pigs (n=18) was investigated by pcr. in addition, shiga toxin (stx) ... | 2005 | 16125342 |
| inhibition of secretory phospholipase a(2) enzyme by bilirubin: a new role as endogenous anti-inflammatory molecule. | bilirubin is a powerful antioxidant that suppresses the inflammatory process. however its interaction with proinflammatory pla(2) enzyme is not known. inhibition of several secretory phospholipase a(2) (spla(2)) enzyme activities by bilirubin was studied using (14)c-oleate labeled escherichia coli as substrate. bilirubin inhibits purified spla(2) enzyme from vipera russellii and naja naja venom and partially purified spla(2) enzymes from human ascitic fluid, pleural fluid and normal serum in a d ... | 2005 | 16132704 |
| effect of pooling bovine fecal samples on the sensitivity of detection of e. coli o157:h7. | to assess the effect of pooling fecal samples on the sensitivity of detection of e. coli o157:h7, 12 calves, inoculated orally with 10(8)cfu per calf of nalidixic acid resistant e. coli o157:h7, were used to provide positive fecal samples. after inoculation, calves were sampled twice weekly. negative fecal samples were from calves at a local dairy. samples from inoculated calves were incubated without pooling or were mixed with known negative fecal samples in a 1:4 ratio or a 2:3 ratio (positive ... | 2005 | 16140477 |
| nationwide microbiological baseline data collected by sponge sampling during 1997 and 1998 for cattle, swine, turkeys, and geese. | during 1997 and 1998, the u.s. food safety and inspection service completed nationwide microbiological baseline studies on four separate categories of livestock and poultry. data were collected by sponge-sampling techniques. these studies were designed to provide nationwide estimates of the prevalence of salmonella and prevalence and concentrations of escherichia coli in cattle (n = 1,881), swine (n = 2,127), turkeys (n = 1,396), and geese (n = 102) in establishments under federal inspection. sa ... | 2005 | 16161683 |
| amyloid fibril formation of alpha-synuclein is accelerated by preformed amyloid seeds of other proteins: implications for the mechanism of transmissible conformational diseases. | alpha-synuclein is one of the causative proteins of familial parkinson disease, which is characterized by neuronal inclusions named lewy bodies. lewy bodies include not only alpha-synuclein but also aggregates of other proteins. this fact raises a question as to whether the formation of alpha-synuclein amyloid fibrils in lewy bodies may occur via interaction with fibrils derived from different proteins. to probe this hypothesis, we investigated in vitro fibril formation of human alpha-synuclein ... | 2005 | 16162499 |
| therapeutic use of phage cocktail for controlling escherichia coli o157:h7 in gastrointestinal tract of mice. | to investigate the therapeutical use of phage mixture for controlling gastrointestinal escherichia coli o157:h7 cells, in vitro and in vivo experiments were conducted. three phages, sp15, sp21, and sp22 were selected from 26 phage stock screened from feces of stock animals and sewage influent. addition of single or binary phage to the e. coli cell batch-culture reduced the turbidity of the culture. however, reascend of the turbidity due to the appearance of phage resistance cell was observed. on ... | 2005 | 16243277 |
| use of immunomagnetic separation technique and polymerase chain reaction for detection of verocytotoxin-producing escherichia coli in cattle at slaughter. | 2005 | 16244985 | |
| experimental comparison of excision and swabbing microbiological sampling methods for carcasses. | bovine sides, ovine carcasses, and porcine carcasses were individually inoculated by dipping in various suspensions of a marker organism (escherichia coli k-12 or pseudomonas fluorescens), alone or in combination with two meat-derived bacterial strains, and were sampled by two standard methods: cotton wet-dry swabbing and excision. the samples were examined for bacterial counts on plate count agar (pca plate counts) and on violet red brilliant green agar (vrbga plate counts) by standard internat ... | 2005 | 16245724 |
| potential for recombinant babesia bovis antigens to protect against a highly virulent isolate. | two antigens from babesia bovis,12d3 and 11c5, were expressed and purified as recombinant proteins in escherichia coli and used to vaccinate groups of six babesia-susceptible cattle. these were subsequently challenged with a highly virulent strain of b. bovis. all cattle showed symptoms of disease and most required treatment. cattle vaccination groups receiving either 12d3 or 11c5 or a combination of both, reduced parasitaemia by approximately fourfold and a number of individual animals appeared ... | 2005 | 16255742 |
| enzymatic redesigning of biologically active heparan sulfate. | heparan sulfate carries a wide range of biological activities, regulating blood coagulation, cell differentiation, and inflammatory responses. the sulfation patterns of the polysaccharide are essential for the biological activities. in this study, we report an enzymatic method for the sulfation of multimilligram amounts of heparan sulfate with specific functions using immobilized sulfotransferases combined with a 3'-phosphoadenosine 5'-phosphosulfate regeneration system. by selecting appropriate ... | 2005 | 16260789 |
| cloning, biochemical properties, and distribution of mycobacterial haloalkane dehalogenases. | haloalkane dehalogenases are enzymes that catalyze the cleavage of the carbon-halogen bond by a hydrolytic mechanism. genomes of mycobacterium tuberculosis and m. bovis contain at least two open reading frames coding for the polypeptides showing a high sequence similarity with biochemically characterized haloalkane dehalogenases. we describe here the cloning of the haloalkane dehalogenase genes dmba and dmbb from m. bovis 5033/66 and demonstrate the dehalogenase activity of their translation pro ... | 2005 | 16269704 |
| antibiotic susceptibility of mammalian mitochondrial translation. | all medically useful antibiotics should have the potential to distinguish between target microbes (bacteria) and host cells. although many antibiotics that target bacterial protein synthesis show little effect on the translation machinery of the eukaryotic cytoplasm, it is unclear whether these antibiotics target or not the mitochondrial translation machinery. we employed an in vitro translation system from bovine mitochondria, which consists of mitochondrial ribosomes and mitochondrial elongati ... | 2005 | 16271719 |
| spatial and temporal epidemiology of sporadic human cases of escherichia coli o157 in scotland, 1996-1999. | in scotland, between 1995 and 2000 there were between 4 and 10 cases of illness per 100000 population per year identified as being caused by escherichia coli o157, whereas in england and wales there were between 1 and 2 cases per 100000 population per year. within scotland there is significant regional variation. a cluster of high rate areas was identified in the northeast of scotland and a cluster of low rate areas in central-west scotland. temporal trends follow a seasonal pattern whilst spati ... | 2005 | 16274499 |
| phosphorus, sediment, and escherichia coli loads in unfenced streams of the georgia piedmont, usa. | contamination of unfenced streams with p, sediments, and pathogenic bacteria from cattle (bos taurus) activity may be affected by the availability of shade and alternative water sources. the objectives of this study were to evaluate water quality in two streams draining tall fescue (festuca arundinacea schreb.)-common bermudagrass (cynodon dactylon l.) pastures with different shade distribution, and to quantify the effects of alternative water sources on stream water quality. for 3 yr, loads of ... | 2005 | 16275730 |
| antimicrobial susceptibility of pathogenic escherichia coli isolated from sick cattle and pigs in japan. | we examined the 12 antimicrobial susceptibilities of 175 e. coli isolates from sick cattle and pigs by an agar dilution method. resistance was found in 78.3% of isolates for oxytetracycline, 70.3% of isolates for dihydrostreptomycin, and 49.1% of isolates for ampicillin. when compared with healthy animals reported by kijima-tanaka et al., resistance rates for 11 antimicrobial agents were higher in sick cattle than in healthy cattle, and resistance rates for all antimicrobial agents were higher i ... | 2005 | 16276055 |
| multivalent display system on filamentous bacteriophage pvii minor coat protein. | the systems for display of foreign peptides and polypeptides on filamentous bacteriophage have exploited genetic fusion to all of the five coat proteins. multivalent display systems allowing selection of low affinity antibody fragments have been devised for fusions to gene iii. however, since piii has to interact with the bacterial receptors during the infection process, reduced infectivity can be observed. alternative display systems utilizing other coat protein have been examined. these, howev ... | 2005 | 16277988 |
| assembly of tbetari:tbetarii:tgfbeta ternary complex in vitro with receptor extracellular domains is cooperative and isoform-dependent. | transforming growth factor-beta (tgfbeta) isoforms initiate signaling by assembling a heterotetrameric complex of paired type i (tbetari) and type ii (tbetarii) receptors on the cell surface. because two of the ligand isoforms (tgfbetas 1, 3) must first bind tbetarii to recruit tbetari into the complex, and a third (tgfbeta2) requires a co-receptor, assembly is known to be sequential, cooperative and isoform-dependent. however the source of the cooperativity leading to recruitment of tbetari and ... | 2005 | 16289576 |
| ecology of escherichia coli o157:h7 in commercial dairies in southern alberta. | shedding of escherichia coli o157:h7 was monitored monthly over a 1-yr period by collecting pooled fecal pats (fecal) and manila ropes orally accessed for 4 h (rope) from multiple pens of cattle in 5 commercial dairies in southern alberta, canada. using immunomagnetic separation, e. coli o157:h7 was isolated from cows on 4 of the dairies and from 13.5% of fecal and 1.1% of rope samples. pulsed-field gel electrophoresis of xbai- and spei-digested bacterial dna of the 65 isolates produced 23 uniqu ... | 2005 | 16291636 |
| slaughterhouse survey of pyelonephritis in dairy cows. | twenty-one rejected kidneys from 2426 slaughtered dairy cows (0.87 per cent) had gross signs of pyelonephritis that were confirmed by histopathology. in all the kidneys the findings were consistent with a chronic rather than an acute infection. one species of bacteria was cultured from 12 of the kidneys and two species of bacteria were cultured from six. the most commonly isolated bacteria were escherichia coli, from eight kidneys, arcanobacterium pyogenes, from seven kidneys and corynebacterium ... | 2005 | 16299366 |
| methods for recovering escherichia coli o157:h7 from cattle fecal, hide, and carcass samples: sensitivity and improvements. | the meats research unit (mru) methods, developed by mru scientists of the u.s. meat animal research center, have been used to study the prevalence of escherichia coli o157:h7 in cattle carcass, hide, and fecal samples. the sensitivity of these methods for recovery of injured e. coli o157:h7 cells from inoculated and uninoculated samples was determined, and potential improvements to these methods were evaluated. when using the conventional mru methods, 91% of the pre-evisceration carcass samples ... | 2005 | 16300061 |
| antibiotic resistance and hypermutability of escherichia coli o157 from feedlot cattle treated with growth-promoting agents. | in a longitudinal study (165 days), we investigated the effect of growth-promoting agents (monensin and trenbolone acetate-estradiol) and an antibiotic (oxytetracycline) on the incidence in feedlot steers of escherichia coli o157, including antibiotic-resistant and hypermutable isolates. eighty steers in 16 pens were treated with eight combinations of promoters, and each treatment was duplicated. fecal samples were collected at nine different sampling times for detection of e. coli o157. overall ... | 2005 | 16300081 |
| [identification on the immunogenic activity of recombinant rltb/ctb-lipl41/ 1 to leptospira interrogans and detection of lipl41 gene with its production]. | to construct prokaryotic expression systems of ltb/ctb-lipl41/1 fusion genes, identify immunogenic and adjuvant activities of the products as well as to understand the frequencies of lipl41 gene that carrying and expressing in l. interrogans wild strains and specific antibody levels in sera from patients with leptospirosis. | 2005 | 16390012 |
| modeling and predicting the simultaneous growth of escherichia coli o157:h7 and ground beef background microflora for various enrichment protocols. | the simultaneous growth of escherichia coli o157:h7 (o157) and the ground beef background microflora (bm) was described in order to characterize the effects of enrichment factors on the growth of these organisms. the different enrichment factors studied were basal medium (trypticase soy broth and e. coli broth), the presence of novobiocin in the broth, and the incubation temperature (37 degrees c or 40 degrees c). bm and o157 kinetics were simultaneously fitted by using a competitive growth mode ... | 2006 | 16391052 |
| prevalence and virulence factors of escherichia coli serogroups o26, o103, o111, and o145 shed by cattle in scotland. | a national survey was conducted to determine the prevalence of escherichia coli o26, o103, o111, and o145 in feces of scottish cattle. in total, 6,086 fecal pats from 338 farms were tested. the weighted mean percentages of farms on which shedding was detected were 23% for e. coli o26, 22% for e. coli o103, and 10% for e. coli o145. the weighted mean prevalences in fecal pats were 4.6% for e. coli o26, 2.7% for e. coli o103, and 0.7% for e. coli o145. no e. coli o111 was detected. farms with catt ... | 2006 | 16391103 |
| quantitative analysis and evaluation of the solubility of hydrophobic proteins recovered from brain, heart and urine using uv-visible spectrophotometry. | there is a need for a simple method that can directly quantify hydrophobic proteins. uv-visible spectrophotometry was applied in the present study for this purpose. absorbance at lambda = 280 nm (a280) was detected for both escherichia coli membrane proteins and bovine serum albumin, whereas absorbance at lambda = 620 nm (a620) was only detected for e. coli membrane proteins. the a620 values of the brain samples were greater than those of heart samples when equal concentrations were used, regard ... | 2006 | 16402178 |
| geographical variation in antibiotic resistance profiles of escherichia coli isolated from swine, poultry, beef and dairy cattle farm water retention ponds in florida. | the aim of this study was to assess geographical variation in multiple antibiotic resistance (mar) profiles of livestock escherichia coli as well as to evaluate the ability of mar profiles to differentiate sources of faecal pollution. | 2006 | 16405684 |
| super-shedding cattle and the transmission dynamics of escherichia coli o157. | the prevalence of escherichia coli o157 displays striking variability across the scottish cattle population. on 78% of farms, in a cross-sectional survey of 952, no shedding of e. coli o157 was detected, but on a small proportion, approximately 2%, very high prevalences of infection were found (with 90-100% of pats sampled being positive). we ask whether this variation arises from the inherent stochasticity in transmission dynamics or whether it is a signature of underlying heterogeneities in th ... | 2006 | 16409660 |
| differentiation of faecal escherichia coli from humans and animals by multiple antibiotic resistance analysis. | multiple antibiotic resistance (mar) was performed on 128 escherichia coli isolates, recovered from faecal samples of humans and animals (cattle, goat, sheep) to determine and compare their antibiotic resistance patterns and to evaluate them statistically in order to specify the source of the faecal material. | 2006 | 16411923 |
| salvage of focal cerebral ischemic damage by transfusion of high o2-affinity recombinant hemoglobin polymers in mouse. | cell-free hemoglobin solutions with high oxygen affinity might be beneficial for selectively delivering oxygen to ischemic tissue. a recombinant hybrid hemoglobin molecule was designed using the human alpha-subunit and the bovine beta-subunit, with placement of surface cysteines to permit disulfide bond polymerization of the tetramers. the resulting protein generated from an escherichia coli expression system had a molecular mass >1 mda, a p50 of approximately 3 torr, and a cooperativity of n = ... | 2006 | 16424069 |
| acute experimental mastitis is not causal toward the development of energy-related metabolic disorders in early postpartum dairy cows. | twenty holstein cows in early lactation (7 d in milk) were administered 100 microg of escherichia coli lipopolysaccharide (lps) dissolved in 10 ml of sterile 0.9% nacl saline (treatment; trt) or 10 ml of sterile saline (control) into both right mammary quarters to test the hypothesis that acute experimental mastitis would have negative impacts on aspects of energy metabolism that might lead to the development of metabolic disorders. a primed continuous intravenous infusion (14-micromol/kg of bw ... | 2006 | 16428629 |
| escherichia coli with anti-o157:h7 activity isolated from bovine colon. | to isolate bacteria from bovine gastrointestinal tract and investigate their inhibitory effect on escherichia coli o157:h7 in vitro. | 2006 | 16430515 |
| comparison of effects of high-pressure processing and heat treatment on immunoactivity of bovine milk immunoglobulin g in enriched soymilk under equivalent microbial inactivation levels. | immunoglobulin-rich foods may provide health benefits to consumers. to extend the refrigerated shelf life of functional foods enriched with bovine immunoglobulin g (igg), nonthermal alternatives such as high-pressure processing (hpp) may offer advantages to thermal processing for microbial reduction. to evaluate the effects of hpp on the immunoactivity of bovine igg, a soymilk product enriched with milk protein concentrates, derived from dairy cows that were hyperimmunized with 26 human pathogen ... | 2006 | 16448177 |
| selection of an escherichia coli o157:h7 bacteriophage for persistence in the circulatory system of mice infected experimentally. | a bacteriophage lytic for escherichia coli o157:h7 was isolated from bovine manure. following in-vivo selection, the phage acquired the capacity to persist in the circulatory system of mice for at least 38 days. when mice were infected experimentally with e. coli o157:h7 (10(7) cfu/mouse), simultaneous injection of the mice with phage (10(8) pfu/mouse) cleared e. coli o157:h7 from the mice within 48 h. | 2006 | 16451412 |
| role of coresistance in the development of resistance to chloramphenicol in escherichia coli isolated from sick cattle and pigs. | to determine the cause of persistent resistance to chloramphenicol (cp) after the ban on its use in food-producing animals in several countries. | 2006 | 16454626 |
| bedding and within-pen location effects on feedlot pen runoff quality using a rainfall simulator. | soluble salts, nutrients, and pathogenic bacteria in feedlot-pen runoff have the potential to cause pollution of the environment. a 2-yr study (1998-1999) was conducted at a beef cattle (bos taurus) feedlot in southern alberta, canada, to determine the effect of bedding material [barley (hordeum vulgare l.) straw versus wood chips] and within-pen location on the chemical and bacterial properties of pen-floor runoff. runoff was generated with a portable rainfall simulator and analyzed for chemica ... | 2006 | 16455851 |
| assessing the efficacy of within-animal control strategies against e. coli o157: a simulation study. | a stochastic simulation model was used to assess the efficacy of potential measures to control the levels of escherichia coli o157 within the bovine host. the model described e. coli o157 population sizes at several sites along the bovine gut and therefore only interventions that operate at an individual animal level could be evaluated. in order to use the model to evaluate the control strategies, it was necessary to make assumptions about how each strategy affected e. coli o157 populations in v ... | 2006 | 16464511 |
| cesarean section and ovariohysterectomy after severe uterine torsion in four cows. | to report a technique for intra-partum ovariohysterectomy (ovh) in cows after severe uterine compromise after uterine torsion. | 2006 | 16472302 |
| characterization of cytokine expression in milk somatic cells during intramammary infections with escherichia coli or staphylococcus aureus by real-time pcr. | the expression of inflammatory cytokines, including interleukin (il)-6, il-8, il-12, granulocyte macrophage-colony stimulating factor (gm-csf), tumor necrosis factor (tnf)-alpha, and interferon (ifn)-gamma, by milk somatic cells was characterized by real-time polymerase chain reaction (pcr) in dairy cows experimentally challenged with either e. coli (n = 8) or s. aureus (n = 8). the mrna abundance of a target gene was calibrated with that of a reference gene (beta-actin) and expressed as fold of ... | 2006 | 16472521 |
| fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture. | visualization of the formation and orientation of collagen fibers in tissue engineering experiments is crucial for understanding the factors that determine the mechanical properties of tissues. in this study, collagen-specific fluorescent probes were developed using a new approach that takes advantage of the inherent specificity of collagen binding protein domains present in bacterial adhesion proteins (cna35) and integrins (gst-alpha1i). both collagen binding domains were obtained as fusion pro ... | 2006 | 16476406 |
| biochemical characterization of human enteropeptidase light chain. | the synthetic gene encoding human enteropeptidase light chain (l-hep) was cloned into plasmid pet-32a downstream from the gene of fusion partner thioredoxin immediately after the dna sequence encoding the enteropeptidase recognition site. the fusion protein thioredoxin (trx)/l-hep was expressed in escherichia coli bl21(de3). autocatalytic cleavage of the fusion protein and activation of recombinant l-hep were achieved by solubilization of inclusion bodies and refolding of trx/l-hep fusion protei ... | 2006 | 16489914 |
| biosynthetic 13c labeling of aromatic side chains in proteins for nmr relaxation measurements. | site-specific 13c labeling offers a desirable means of eliminating unwanted relaxation pathways and coherent magnetization transfer in nmr relaxation experiments. here we use [1-13c]-glucose as the sole carbon source in the growth media for protein overexpression in escherichia coli. the approach results in specific incorporation of 13c at isolated positions in the side chains of aromatic amino acids, which greatly simplifies the measurements and interpretation of 13c relaxation rates in these s ... | 2006 | 16492013 |
| prevalence and characterization of escherichia coli o157 isolates from minnesota dairy farms and county fairs. | samples were collected from 26 organic and conventional farms and 12 county fairs in minnesota during 2001 and 2002 to identify the presence of escherichia coli o157. immunomagnetic separation was used for isolation of e. coli o157. isolates were further characterized by the presence of virulence marker genes (stx1, stx2, eaea, e-hly, katp, etpd, and espp), antimicrobial susceptibility profiles, and genotypes. during 2001, e. coli o157 was isolated from 16 (5.2%) of 305 fecal samples and from 7 ... | 2006 | 16496562 |
| comparison of e. coli o157 and shiga toxin-encoding genes (stx) prevalence between ohio, usa and norwegian dairy cattle. | environmental and food contamination with shiga toxin-producing escherichia coli (stec) pose a threat to public health worldwide, with notable geographic differences in incidence of human disease caused by these organisms. the prevalence of e. coli o157 and total stx-positive specimens collected from mature dairy cattle in ohio and norwegian dairy farms was compared using identical laboratory methods in a cross-sectional survey. e. coli o157 was isolated from 5/750 (0.66%) of ohio dairy cows fro ... | 2006 | 16504323 |
| phosphorus and sediment loss in a catchment with winter forage grazing of cropland by dairy cattle. | the loss of phosphorus and sediment to surface waters can impair their quality. it was hypothesized that the practice of winter grazing dairy cattle on cropland of moderate slope (5-20%) would exacerbate the loss of p and suspended sediment (ss) from land to water. in a small (4.3 ha) catchment two flumes were installed, upstream and downstream of one field (about 2 ha) that had been cropped for 2 yr and grazed in winter (june-july) by dairy cattle. flow proportional samples were taken and measu ... | 2006 | 16510702 |
| the effect of an experimentally induced acute mastitis on the test results of an ostertagia ostertagi milk elisa. | antibody levels against ostertagia ostertagi in the milk are a promising parameter to identify dairy cows or herds with production losses due to gastrointestinal nematodes. however, milk antibody levels can be influenced by udder-related factors. in this study, the effect of an experimentally induced mastitis on the test results (odr) of a milk o. ostertagi elisa was investigated. twenty-five cows that were naturally infected with gastrointestinal nematodes, were inoculated in their left udder q ... | 2006 | 16300898 |
| a herd level analysis of urinary tract infection in dairy cattle. | cystitis, urethritis and pyelonephritis in cattle most commonly result from ascending urinary tract infection with corynebacterium renale, corynebacterium cystidis, corynebacterium pilosum or escherichia coli. we describe the clinical, bacteriological, clinical-pathological and epidemiological findings in a dairy cattle herd with urinary tract infection (uti). blood and urine samples from 17 calves and 19 cows were submitted to laboratory examinations. depression, muscle wasting, weakness and fr ... | 2006 | 16310382 |
| inhibitory effects of microencapsulated allyl isothiocyanate (ait) against escherichia coli o157:h7 in refrigerated, nitrogen packed, finely chopped beef. | allyl isothiocyanate (ait) is an effective inhibitor of various pathogens, but its use in the food industry is limited by its volatility and pungency. the objective of this study was to overcome the volatility of ait by microencapsulation and evaluate its antimicrobial effectiveness against escherichia coli o157:h7 in chopped beef. chopped beef was aseptically prepared and inoculated with a five-strain cocktail of e. coli o157:h7 to yield 4 or 8 log10 cfu/g. ait was microencapsulated in gum acac ... | 2006 | 16266766 |
| differential detection of turkey coronavirus, infectious bronchitis virus, and bovine coronavirus by a multiplex polymerase chain reaction. | the objective of the present study was to develop a multiplex polymerase chain reaction (pcr) method for differential detection of turkey coronavirus (tcov), infectious bronchitis coronavirus (ibv), and bovine coronavirus (bcov). primers were designed from conserved or variable regions of nucleocapsid (n) or spike (s) protein gene among tcov, ibv, and bcov and used in the same pcr reaction. reverse transcription followed by the pcr reaction was used to amplify a portion of n or s gene of the cor ... | 2006 | 16137773 |
| association between indicators of cattle density and incidence of paediatric haemolytic-uraemic syndrome (hus) in children under 15 years of age in france between 1996 and 2001: an ecological study. | over the past years shiga-like toxin-producing escherichia coli (stec) o157:h7 emerged as an important cause of severe gastrointestinal illnesses and haemolytic-uraemic syndrome (hus) with up to 10% of children infected with stec developing hus. we conducted a geographical ecological study using the district as the statistical unit. for each district, we estimated the incidence of hus among children <15 years for the period 1996-2001 from national hus surveillance data and data obtained on cattl ... | 2006 | 16371171 |
| in vivo expression of the antimicrobial defensin and lactoferrin proteins allowed by the strategic insertion of introns adequately spliced. | a major limitation of conventional shuttle expression system, when cloning a bactericidal gene, is the basal expression level in bacteria, which is lethal. although the expression level is low, the bactericidal feature inherent to the molecule leads to subsequent failure to recover intact transformants when the related gene is cloned into a conventional expression vector. contrary to popular belief, the human cytomegalovirus immediate-early region 1 promoter (cmv), which is to date one of the mo ... | 2006 | 16516411 |
| assembly of cs1 pili: the role of specific residues of the major pilin, cooa. | cs1 pili are important virulence factors of enterotoxigenic escherichia coli strains associated with human diarrheal disease. they are the prototype for a family of pili that share extensive sequence similarity among their structural and assembly proteins. only four linked genes, coob, cooa, cooc, and cood, are required to produce cs1 pili in e. coli k-12. to identify amino acids important for the function of the major pilin cooa, we used alanine substitution mutagenesis targeting conserved resi ... | 2006 | 16352839 |
| influence of sex steroids on the viability and cd11b, cd18 and cd47 expression of blood neutrophils from dairy cows in the last month of gestation. | in the period around parturition, cows experience an increased susceptibility for the development of escherichia coli mastitis. this increased susceptibility has been correlated with a decreased functionality of neutrophils. in the current study, it is suggested that the decreased neutrophil functionality may be induced by the extensive alterations in sex steroid levels occurring around parturition. it was first hypothesized that 17beta-estradiol and progesterone influence the viability, apoptos ... | 2006 | 16336925 |