Publications

rna-seq analysis identifies new genes regulated by the histone-like nucleoid structuring protein (h-ns) affecting vibrio cholerae virulence, stress response and chemotaxis.	the histone-like nucleoid structuring protein (h-ns) functions as a transcriptional silencer by binding to at-rich sequences at bacterial promoters. however, h-ns repression can be counteracted by other transcription factors in response to environmental changes. the identification of potential toxic factors, the expression of which is prevented by h-ns could facilitate the discovery of new regulatory proteins that may contribute to the emergence of new pathogenic variants by anti-silencing. vibr ...	2015	25679988
synthesis and molecular structure of the 5-methoxycarbonylpentyl α-glycoside of the upstream, terminal moiety of the o-specific polysaccharide of vibrio cholerae o1, serotype inaba.	the trimethylsilyl trifluoromethanesulfonate (tmsotf)-catalyzed reaction of methyl 6-hydroxyhexanoate with 3-o-benzyl-4-(2,4-di-o-acetyl-3-deoxy-l-glycero-tetronamido)-4,6-dideoxy-2-o-levulinoyl-α-d-mannopyranosyl trichloroacetimidate followed by a two-step deprotection (hydrogenolysis over pd/c catalyst and zemplén deacylation, to simultaneously remove the acetyl and levulinoyl groups) gave 5-(methoxycarbonyl)pentyl 4-(3-deoxy-l-glycero-tetronamido)-4,6-dideoxy-α-d-mannopyranoside. the structur ...	2015	25679049
in silico phylogenetic analysis of vibrio cholerae isolates based on three housekeeping genes.	vibrio cholera, a gram-negative bacterium, has been categorised into clinical and environmental species. phylogenetic studies have been performed to investigate the relationships of the v. cholerae populations in worldwide. in this study, phylogenetic relationship between v. cholerae isolates from iran and other regions of the world was determined, based on three housekeeping genes analysis. results for iranian strains showed that congruency of asd and hlya phylogenetic trees were remarkably hig ...	2015	25869320
enhanced resistance to several abiotic stresses in vibrio cholerae during starvation.	in this study, we investigated whether vibrio cholerae cells exposed to nutrient-limited conditions developed resistance to abiotic stresses because of which most, if not all, starved cells turn coccoid in shape and exhibited marked resistance to freezing, low ph, and chlorine, but not against bile.	2015	25866118
plant-based production of two chimeric monoclonal igg antibodies directed against immunodominant epitopes of vibrio cholerae lipopolysaccharide.	we have produced and characterized two chimeric human igg1 monoclonal antibodies that bind different immunodominant epitopes on vibrio cholerae lipopolysaccharide (lps). mab 2d6 igg1 recognizes ogawa o-polysaccharide antigen, while mab zac-3 igg1 recognizes core/lipid a moiety of ogawa and inaba lps. both antibodies were expressed using a nicotiana benthamiana-based rapid antibody-manufacturing platform (ramp) and evaluated in vitro for activities associated with immunity to v. cholerae, includi ...	2015	25865265
nitric oxide is a potent inhibitor of the cbb(3)-type heme-copper oxidases.	c-type heme-copper oxidases terminate the respiratory chain in many pathogenic bacteria, and will encounter elevated concentrations of no produced by the immune defense of the host. thus, a decreased sensitivity to no in c-type oxidases would increase the survival of these pathogens. here we have compared the inhibitory effect of no in c-type oxidases to that in the mitochondrial a-type. we show that o2-reduction in both the rhodobacter sphaeroides and vibrio cholerae c-type oxidases is strongly ...	2015	25862499
clonal dissemination of a single vibrio cholerae o1 biotype el tor strain in sistan-baluchestan province of iran during 2013.	although much is known about the mechanisms affecting cholera spread, cholera outbreaks occur annually in iran. the aim of this study was to characterize and assess the clonal correlation of strains obtained from an outbreak in 2013 in iran. thirty-three strains of vibrio cholerae were isolated from stool sample of patients majority of them belonged to afghan nationality. pcr and sequencing analysis was performed to characterize virulence and resistance associates genes and cassettes. clonality ...	2015	25862465
liposome reconstitution and transport assay for recombinant transporters.	secondary active transporters are responsible for the cellular uptake of many biologically important molecules, including neurotransmitters, nutrients, and drugs. because of their physiological and clinical importance, a method for assessing their transport activity in vitro is necessary to gain a better understanding of how these transporters function at the molecular level. in this chapter, we describe a protocol for reconstituting the concentrative nucleoside transporter from vibrio cholerae ...	2015	25857791
increased isolation frequency of toxigenic vibrio cholerae o1 from environmental monitoring sites in haiti.	since the identification of the first cholera case in 2010, the disease has spread in epidemic form throughout the island nation of haiti; as of 2014, about 700,000 cholera cases have been reported, with over 8,000 deaths. while case numbers have declined, the more fundamental question of whether the causative bacterium, vibrio cholerae has established an environmental reservoir in the surface waters of haiti remains to be elucidated. in a previous study conducted between april 2012 and march 20 ...	2015	25853552
high production of neuraminidase by a vibrio cholerae non-o1 strain--the first possible alternative to toxigenic producers.	vibrio cholerae neuraminidase (vcna) is widely used in biochemical and medical research, in processes for preparing homogenous sialoconjugates, and in the pharmaceutical industry. its production by non-toxigenic strains is quite desirable, in order to avoid the expensive safety measures. here, we report the first method for highly effective production of a novel, purified v. cholerae extracellular neuraminidase from a non-toxigenic strain. the enzyme is highly active, and its properties, as well ...	2015	25805019
scanning the landscape of genome architecture of non-o1 and non-o139 vibrio cholerae by whole genome mapping reveals extensive population genetic diversity.	historically, cholera outbreaks have been linked to v. cholerae o1 serogroup strains or its derivatives of the o37 and o139 serogroups. a genomic study on the 2010 haiti cholera outbreak strains highlighted the putative role of non o1/non-o139 v. cholerae in causing cholera and the lack of genomic sequences of such strains from around the world. here we address these gaps by scanning a global collection of v. cholerae strains as a first step towards understanding the population genetic diversity ...	2015	25794000
identification and characterisation of a novel plasmid-mediated quinolone resistance gene, qnrvc7, in vibrio cholerae of seafood origin.		2015	25769785
multidrug-resistant vibrio cholerae o1 was responsible for a cholera outbreak in 2013 in bagalkot, north karnataka.	cholera is a major cause of illness in the developing world. during the monsoon season, small sporadic clusters of cholera cases are reported on an annual basis in karnataka, india. during the monsoons of 2013, there was a cholera outbreak in badami, a remote area of bagalkot district in karnataka. the multi-drug-resistant vibrio cholerae o1 serotype ogawa was found to be responsible for this outbreak. on 5 august 2013, a 30-year-old woman presented with severe dehydration and watery diarrhea at ...	2015	25766606
toxigenic vibrio cholerae identified in estuaries of tanzania using pcr techniques.	the current study assessed the occurrence of the vibrio cholerae serogroups o1 and o139 in environmental samples along salinity gradients in three selected estuaries of tanzania both through culture independent methods and by cultured bacteria. occurrence of v. cholerae was determined by pcr targeting the v. cholerae outer membrane protein gene ompw. furthermore, the presence of toxigenic strains and serogroups o1 and o139 was determined using multiplex pcr with specific primers targeting the ch ...	2015	25743072
correction for herrera et al., the vibrio cholerae vpra-vprb two-component system controls virulence through endotoxin modification.		2015	25736885
mechanism for inhibition of vibrio cholerae toxt activity by the unsaturated fatty acid components of bile.	the gram-negative curved bacillus vibrio cholerae causes the severe diarrheal illness cholera. during host infection, a complex regulatory cascade results in production of toxt, a dna-binding protein that activates the transcription of major virulence genes that encode cholera toxin (ct) and toxin-coregulated pilus (tcp). previous studies have shown that bile and its unsaturated fatty acid (ufa) components reduce virulence gene expression and therefore are likely important signals upon entering ...	2015	25733618
identifying the most sensitive and specific sign and symptom combinations for cholera: results from an analysis of laboratory-based surveillance data from haiti, 2012-2013.	since october 2010, over 700,000 cholera cases have been reported in haiti. we used data from laboratory-based surveillance for diarrhea in haiti to evaluate the sensitivity, specificity, and positive (ppv) and negative predictive values (npv) of the cholera case definitions recommended by the world health organization (who). from april 2012 to may 2013, we tested 1,878 samples from hospitalized patients with acute watery diarrhea; 1,178 (62.7%) yielded vibrio cholerae o1. the sensitivity and sp ...	2015	25732682
a novel triplex quantitative pcr strategy for quantification of toxigenic and nontoxigenic vibrio cholerae in aquatic environments.	vibrio cholerae is a severe human pathogen and a frequent member of aquatic ecosystems. quantification of v. cholerae in environmental water samples is therefore fundamental for ecological studies and health risk assessment. beside time-consuming cultivation techniques, quantitative pcr (qpcr) has the potential to provide reliable quantitative data and offers the opportunity to quantify multiple targets simultaneously. a novel triplex qpcr strategy was developed in order to simultaneously quanti ...	2015	25724966
structure of the type vi secretion system contractile sheath.	bacteria use rapid contraction of a long sheath of the type vi secretion system (t6ss) to deliver effectors into a target cell. here, we present an atomic-resolution structure of a native contracted vibrio cholerae sheath determined by cryo-electron microscopy. the sheath subunits, composed of tightly interacting proteins vipa and vipb, assemble into a six-start helix. the helix is stabilized by a core domain assembled from four β strands donated by one vipa and two vipb molecules. the fold of i ...	2015	25723169
rapid proliferation of vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae during freshwater flash floods in french mediterranean coastal lagoons.	vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae of the non-o1/non-o139 serotype are present in coastal lagoons of southern france. in these mediterranean regions, the rivers have long low-flow periods followed by short-duration or flash floods during and after heavy intense rainstorms, particularly at the end of the summer and in autumn. these floods bring large volumes of freshwater into the lagoons, reducing their salinity. water temperatures recorded during sampling (15 to 24° ...	2015	26319881
role of toxs in the proteolytic cascade of virulence regulator toxr in vibrio cholerae.	two of the primary virulence regulators of vibrio cholerae, toxr and tcpp, function together with cognate effector proteins. toxr undergoes regulated intramembrane proteolysis (rip) during late stationary phase in response to nutrient limitation at alkaline ph; however, the specific function of its cognate toxs remains unresolved. in this work, we found that toxr rapidly becomes undetectable in a δtoxs mutant when cultures are exposed to either starvation conditions or after alkaline ph shock in ...	2015	26316386
an electrochemical genosensing assay based on magnetic beads and gold nanoparticle-loaded latex microspheres for vibrio cholerae detection.	an ultrasensitive electrochemical genosensing assay was developed for the sequence-specific detection of vibrio cholerae dna using magnetic beads as the biorecognition surface and gold nanoparticle-loaded latex microspheres (latex-aunps) as a signal-amplified hybridization tag. this biorecognition surface was prepared by immobilizing specific biotinylated capturing probes onto the streptavidin-coupled magnetic beads. fabricating a hybridization tag capable of amplifying the electrochemical signa ...	2015	26310076
signaling beyond punching holes: modulation of cellular responses by vibrio cholerae cytolysin.	pore-forming toxins (pfts) are a distinct class of membrane-damaging cytolytic proteins that contribute significantly towards the virulence processes employed by various pathogenic bacteria. vibrio cholerae cytolysin (vcc) is a prominent member of the beta-barrel pft (beta-pft) family. it is secreted by most of the pathogenic strains of the intestinal pathogen v. cholerae. owing to its potent membrane-damaging cell-killing activity, vcc is believed to play critical roles in v. cholerae pathogene ...	2015	26308054
vibrio cholerae leuo transcription is positively regulated by toxr and contributes to bile resistance.	vibrio cholerae is an aquatic organism and facultative human pathogen that colonizes the small intestine. in the small intestine, v. cholerae is exposed to a variety of antimicrobial compounds, including bile. v. cholerae resistance to bile is multifactorial and includes alterations in the membrane permeability barrier that are mediated by toxr, a membrane-associated transcription factor. toxr has also been shown to be required for activation of the lysr family transcription factor leuo in respo ...	2015	26303831
antimicrobial effects of blueberry, raspberry, and strawberry aqueous extracts and their effects on virulence gene expression in vibrio cholerae.	the antimicrobial effects of aqueous extracts of blueberry, raspberry, and strawberry on 13 pathogenic bacteria were evaluated. the minimum inhibitory concentrations and minimum bactericidal concentrations of the extracts were determined before and after neutralization to ph 7.03 ± 0.15. both gram-positive and gram-negative pathogenic bacteria were selectively inhibited by the non-neutralized berries. blueberry was the best inhibitor, and vibrio and listeria were the most sensitive bacteria. aft ...	2015	26292998
a globally distributed mobile genetic element inhibits natural transformation of vibrio cholerae.	natural transformation is one mechanism of horizontal gene transfer (hgt) in vibrio cholerae, the causative agent of cholera. recently, it was found that v. cholerae isolates from the haiti outbreak were poorly transformed by this mechanism. here, we show that an integrating conjugative element (ice)-encoded dnase, which we name idea, is necessary and sufficient for inhibiting natural transformation of haiti outbreak strains. we demonstrate that idea inhibits this mechanism of hgt in cis via dna ...	2015	26240317
infant rabbit model for diarrheal diseases.	vibrio cholerae is the agent of cholera, a potentially lethal diarrheal disease that remains a significant threat to populations in developing nations. the infant rabbit model of cholera is the only non-surgical small animal model system that closely mimics human cholera. following orogastric inoculation, v. cholerae colonizes the intestines of infant rabbits, and the animals develop severe cholera-like diarrhea. in this unit, we provide a detailed description of the preparation of the v. choler ...	2015	26237109
highly diverse recombining populations of vibrio cholerae and vibrio parahaemolyticus in french mediterranean coastal lagoons.	vibrio parahaemolyticus and vibrio cholerae are ubiquitous to estuarine and marine environments. these two species found in mediterranean coastal systems can induce infections in humans. environmental isolates of v. cholerae (n = 109) and v. parahaemolyticus (n = 89) sampled at different dates, stations and water salinities were investigated for virulence genes and by a multilocus sequence-based analysis (mlsa). v. cholerae isolates were all ctxa negative and only one isolate of v. parahaemolyti ...	2015	26236294
physicochemical constraints of elevated ph affect efficient membrane interaction and arrest an abortive membrane-bound oligomeric intermediate of the beta-barrel pore-forming toxin vibrio cholerae cytolysin.	vibrio cholerae cytolysin (vcc) is a potent membrane-damaging cytotoxic protein. vcc causes permeabilization of the target cell membranes by forming transmembrane oligomeric beta-barrel pores. membrane pore formation by vcc involves following key steps: (i) membrane binding, (ii) formation of a pre-pore oligomeric intermediate, (iii) membrane insertion of the pore-forming motifs, and (iv) formation of the functional transmembrane pore. membrane binding, oligomerization, and subsequent pore-forma ...	2015	26235489
a novel method to combat the cholera epidemic among the romanian army during the balkan war - 1913.	the history of cholera, a specific infection caused by vibrio cholerae, starts in ancient times. the sixth pandemic that began in 1899 and lasted until 1923, started in india and came to eastern europe through russia. the expansion of the epidemic in the balkans was facilitated both by the 2 balkan wars and the first world war. romania, as a participant in these wars was affected by cholera, which was especially common among the army during the balkan war. if the original source of the cholera i ...	2015	26203545
rapid effects of a protective o-polysaccharide-specific monoclonal iga on vibrio cholerae agglutination, motility, and surface morphology.	2d6 is a dimeric monoclonal immunoglobulin a (iga) specific for the nonreducing terminal residue of ogawa o-polysaccharide (ops) of vibrio cholerae. it was previously demonstrated that 2d6 iga is sufficient to passively protect suckling mice from oral challenge with virulent v. cholerae o395. in this study, we sought to define the mechanism by which 2d6 iga antibody protects the intestinal epithelium from v. cholerae infection. in a mouse ligated-ileal-loop assay, 2d6 iga promoted v. cholerae ag ...	2015	25667263
failure of the public health testing program for ballast water treatment systems.	since 2004, an international testing program has certified 53 shipboard treatment systems as meeting ballast water discharge standards, including limits on certain microbes to prevent the spread of human pathogens. we determined how frequently certification tests failed a minimum requirement for a meaningful evaluation, that the concentration of microbes in the untreated (control) discharge must exceed the regulatory limit for treated discharges. in 95% of cases where the result was accepted as ...	2015	25596892
cholera toxin-b (ctxb) antigen expressing salmonella typhimurium polyvalent vaccine exerts protective immune response against vibrio cholerae infection.	live attenuated vaccines are cost effective approach for preventing a broad range of infectious diseases, and thus are of great interest. however, immune-defects can predispose the patient to infections by the vaccine candidate itself. so far, few live vaccine candidates have been designed specifically for immune compromised individuals. recently, we reported a new salmonella typhimurium z234-vaccine strain (periaswamy et al., plos one 2012;7:e45433), which was specifically attenuated in the nad ...	2015	25701672
substrate-dependent activation of the vibrio cholerae vexab rnd efflux system requires vexr.	vibrio cholerae encodes six resistance-nodulation-division (rnd) efflux systems which function in antimicrobial resistance, virulence factor production, and intestinal colonization. among the six rnd efflux systems, vexab exhibited broad substrate specificity and played a predominant role in intrinsic antimicrobial resistance. the vexab system was encoded in an apparent three gene operon that included vexr; which encodes an uncharacterized tetr family regulator. in this work we examined the role ...	2015	25695834
isolation of new delhi metallo-β-lactamase 1-producing vibrio cholerae non-o1, non-o139 strain carrying ctxa, st and hly genes in southern vietnam.	vibrio cholerae non-o1, non-o139 (vc_nag) organisms are universally present in the aquatic environment and regarded as non-pathogenic bacteria. however, considering that they do occasionally induce gastroenteritis, a study of their virulence and antibiotic resistance genes is important. the presence of enteropathogenic genes, including ctxa, vc_nag-specific heat-stable toxin gene (st), hemolysin (hly), and zona occludens toxin (zot) was determined by pcr in 100 vc_nag strains isolated in souther ...	2015	25683557
crystallization of two operator complexes from the vibrio cholerae higba2 toxin-antitoxin module.	the higa2 antitoxin and the higba2 toxin-antitoxin complex from vibrio cholerae were crystallized in complex with their operator box. screening of 22 different dna duplexes led to two crystal forms of higa2 complexes and one crystal form of a higba2 complex. crystals of higa2 in complex with a 17 bp dna duplex belong to space group p3221, with unit-cell parameters a = b = 94.0, c = 123.7 å, and diffract to 2.3 å resolution. the second form corresponding to higa2 in complex with a 19 bp duplex be ...	2015	25664801
expression, purification and preliminary crystallographic analysis of a haem-utilizing protein, hutx, from vibrio cholerae.	vibrio cholerae, the causative agent of cholera, has developed a variety of mechanisms to obtain the limited-availability iron from human hosts. one important method for iron acquisition is through haem-uptake systems. although the transport of haem has been widely studied, the fate of haem once it enters the cytoplasm remains an open question. here, preliminary x-ray crystallographic analysis was performed on hutx, a member of the conserved haem-utilization operon from v. cholerae strain n16961 ...	2015	25664785
stepwise changes in viable but nonculturable vibrio cholerae cells.	many bacterial species are known to become viable but nonculturable (vbnc) under conditions that are unsuitable for growth. in this study, the requirements for resuscitation of vbnc-state vibrio cholerae cells were found to change over time. although vbnc cells could initially be converted to culturable by treatment with catalase or ht-29 cell extract, they subsequently entered a state that was not convertible to culturable by these factors. however, fluorescence microscopy revealed the presence ...	2015	25664673
transfer activation of sxt/r391 integrative and conjugative elements: unraveling the setcd regulon.	integrative and conjugative elements (ices) of the sxt/r391 family have been recognized as key drivers of antibiotic resistance dissemination in the seventh-pandemic lineage of vibrio cholerae. sxt/r391 ices propagate by conjugation and integrate site-specifically into the chromosome of a wide range of environmental and clinical gammaproteobacteria. sxt/r391 ices bear setc and setd, two conserved genes coding for a transcriptional activator complex that is essential for activation of conjugative ...	2015	25662215
microbiological, coliphages and physico-chemical assessments of the umgeni river, south africa.	the water quality of umgeni river in kwazulu-natal (south africa) was investigated from april 2011 to january 2012. indicator bacterial populations, physico-chemical properties, heavy metal contaminants and the presence of coliphages were determined according to standard protocols. the results showed that all sampling points failed to comply with the set guidelines for turbidity, total coliform, faecal coliform and total heterotrophic counts. salmonella spp., shigella spp. and vibrio cholerae we ...	2015	24617904
survivability and molecular variation in vibrio cholerae from epidemic sites in china.	the survival behaviour of vibrio cholerae in cholera epidemics, together with its attributes of virulence-associated genes and molecular fingerprints, are significant for managing cholera epidemics. here, we selected five strains representative of v. cholerae o1 and o139 involved in cholera events, examined their survival capacity in large volumes of water sampled from epidemic sites of a 2005 cholera outbreak, and determined virulence-associated genes and molecular subtype changes of the surviv ...	2015	24650354
characterization of the vibrio cholerae extracellular matrix: a top-down solid-state nmr approach.	bacterial biofilms are communities of bacterial cells surrounded by a self-secreted extracellular matrix. biofilm formation by vibrio cholerae, the human pathogen responsible for cholera, contributes to its environmental survival and infectivity. important genetic and molecular requirements have been identified for v. cholerae biofilm formation, yet a compositional accounting of these parts in the intact biofilm or extracellular matrix has not been described. as insoluble and non-crystalline ass ...	2015	24911407
piezoresistive microcantilever-based dna sensor for sensitive detection of pathogenic vibrio cholerae o1 in food sample.	pathogenic vibrio cholerae produces a cholera toxin which is the cause of a severe diarrheal disease called "cholera". available detection methods, including standard bacteriological test and immuno-based detection, are specific to the suspected pathogenic v. cholerae o1 and o139, but they are not specific to the cholera toxin producible strain. this work combined the polymerase chain reaction (pcr) of cholera toxin gene, ctxa gene, and microcantilever-based dna sensor to improve the sensitivity ...	2015	25113053
molecular simulation of n-acetylneuraminic acid analogs and molecular dynamics studies of cholera toxin-neu5gc complex.	cholera toxin (ct) is an ab5 protein complex secreted by the pathogen vibrio cholera, which is responsible for cholera infection. n-acetylneuraminic acid (neunac) is a derivative of neuraminic acid with nine-carbon backbone. neunac is distributed on the cell surface mainly located in the terminal components of glycoconjugates, and also plays an important role in cell-cell interaction. in our current study, molecular docking and molecular dynamic (md) simulations were implemented to identify the ...	2015	25011464
[letter: teratogenic hazards in antiepileptic therapy].		2016	4815439
[present forensic dentistry, from early times to today].		2016	4898140
late aseptic endophthalmitis after cataract surgery.		2016	4735907
[atypical case of gonococcal bacteremia].		2016	5100586
new directions in information resources for industrial hygiene and occupational health.		2016	4894902
genetic equilibrium under selection.		2016	4864567
another density gradient fractionator.		2016	4711111
the pragmatic utilization of the p-k phenomenon.		2016	5039530
[pathological occlusion].		2016	4898812
primary coliform peritonitis.		2016	5101352
release and return rates for patients in state mental hospitals of maryland.		2016	4959228
[effect of surgical trauma on blood supply, function and innervation of the ureters].		2016	4789264
[practical numerical index of spirometric measurements (fvc, fev 1 , fev percent), eliminating the influence of age and height].		2016	5018508
[precapillary pulmonary arterial hypertension and chronic pulmonary heart disease due to bilharziasis].		2016	5035874
[experience in treating patients with circulatory insufficiency with acedoxin].		2016	5029806
comparative growth of the thermal algae cyanidium caldarium on nitrate and ammonia at different temperatures.		2016	5072724
effect of pesticides applied to the soil on the biological activity of the soil.		2016	5085639
childhood genitourinary neoplasms that do not present as palpable masses.		2016	5079760
[pathogenesis and treatment of vitiligo].		2016	4755955
parkinsonism, l-dopa, and intelligence.		2016	4754685
[use of the peripheral blood leukocyte migration inhibition test in systemic lupus erythematosus].		2016	4804625
[current data on the physiological and pharmacological implications of prostaglandins].		2016	4742706
senile purpura, sublingual hemorrhages, and ascorbic acid.		2016	4864210
bilateral vagal nerve block in evaluation of adams-stokes syndrome in infanty: a case report.		2016	5061722
[electromyographic characterization of the a line].		2016	4870346
metabolism of the heart in health and disease. ii.		2016	4882561
quantum chemical and other theoretical techniques for the understanding of the psychoactive action of the phenothiazines.		2016	4836201
forced-choice adjectival masculinity-femininity scale.		2016	4820512
plasma renin activity in diabetes insipidus.		2016	4802902
[urine collection in a female patient].		2016	4987991
in-vitro lysis of aet-treated normal red cells (pnh-like cells) by normal and cirrhotic sera.		2016	4979897
ocular defects associated with homocystinuria.		2016	4978960
pesticide residues in food. report of the 1968 joint fao-who meeting.		2016	4978471
removal of the 3rd thoracic ganglion for improvement of the blood supply to the lower extremity.		2016	5047973
[electric slag remelting of used electrodes for the casting of dental prosthesis].		2016	4942090
protectionism and provincialism--not the way to build a national association.		2016	5013306
[60th birthday of stanislav pavlovich chepulis].		2016	4926155
[controlled clinical experimentation on delayed-action methaqualone].		2016	4908133
[an unrecognized source of "les faux-monnayeurs"].		2016	4912219
subvocalization: aural and emg feedback in reading.		2016	5095797
[hepato-portal hemodynamics and liver function in heart diseases].		2016	5079456
appendicitis and the abdominal roentgenogram in children.		2016	4800530
immunochemical determination of serum transferrin in patients with anaemia of pregnancy.		2016	4989314
synergism of terramycin to inhibit the reproductivity of aphis fabae scop.		2016	5045184
epidemiological trends in gonorrhoea in recent years.		2016	5014421
[on the diagnostic possibilities of high-speed electrosphygmography in certain cerebrovascular affections].		2016	4957787
studies in non-gonococcal urethritis therapy. the long-term value of tetracycline.		2016	4923984
[semmelweis, pasteur, lister].		2016	4869881
operating room apparel and drapes.		2016	4835674
ultrasonic diagnosis of abdominal aortic aneurysms.		2016	4846789
[rehabilitation of outpatients following surgery on lower limb arteries].		2016	4839702
lattice degeneration in a family: with retinal detachment and cataract.		2016	4743918
blood histamine, basophil counts and trace elements in the schizophrenias.		2016	5052331
proceedings: radiotherapeutic management of basal cell and squamous cell carcinomas of the skin.		2016	4764925
[2 cases of intradural meningioma].		2016	4806371
the physical nature of dermal collagen.		2016	4741418