Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| ion movements in red cells treated with propranolol. | 2015 | 4389297 | |
| [on the use of hearing aids in the pre-school and school age]. | 2015 | 4385769 | |
| [the patient and modern practice]. | 2015 | 4514145 | |
| proceedings: protein-lipid and protein-protein interaction in recombined lipoprotein membranes. | 2015 | 4376784 | |
| the "on-off" side effect of l-dopa. | 2015 | 4440586 | |
| [alkaline phosphatase in the diagnosis of hepatic tumoral metastases]. | 2015 | 4417677 | |
| wilm's tumor: thirty-year review of cases in buffalo. | 2015 | 4363424 | |
| achievements to be expected from new developments in tumour radiotherapy. | 2015 | 4445966 | |
| [factors acting on reminiscence]. | 2015 | 4380790 | |
| biliary lipid composition in normalipidemic and prebeta hyperlipoproteinemic gallstone patients. influence of sucrose feeding of the patients on the biliary lipid composition. | 2015 | 4356589 | |
| [comparative evaluation of nuclear medicine and radiographic examination methods in nephro-urology using the iatros system]. | 2015 | 4367100 | |
| dorsal column stimulator implant. | 2015 | 4345467 | |
| [incompatibilities of antibiotics administered by venous infusion]. | 2015 | 4439354 | |
| space-time clustering of limb defects in cardiff. | 2015 | 4352251 | |
| biometeorological indices. | 2015 | 4438646 | |
| neurologic notes. frontal meningioma with an unusual clinical presentation. | 2015 | 4539903 | |
| [uterine sarcoma of the mulleroblastoma type]. | 2015 | 4359589 | |
| effectiveness of extended aftercare in the treatment of alcoholism. | 2015 | 4351601 | |
| [thromboembolic complications in radiotherapy. influence of depot-eleparon prevention and radium insertion-time on the incidence and course of thromboembolic complications]. | 2015 | 4435692 | |
| gynandromorphs and intersexes in mosquitoes (diptera: culicidae). | 2015 | 4380634 | |
| the co-existence of parathyroid and thyroid disease. i. primary hyperparathyroidism and thyroid carcinoma. | 2015 | 4357240 | |
| can optic atrophy caused by pheniprazine treatment become aggravated by subsequent nialamid treatment? | 2015 | 4380638 | |
| determination of 2,4-dihydroxy-1,4(2h)-benzoxazin-3-one glucosides in corn (zea mays l.). | 2015 | 4363435 | |
| metastatic and paraneoplastic cardiomyopathy. | 2015 | 4275904 | |
| an analysis of road accidents involving child pedestrians. | 2015 | 4442378 | |
| proceedings: interrelationship between swimming performance and chronological age, dental age, skeletal age of age group competitive swimmers. | 2015 | 4364871 | |
| subclinical coagulopathy following amnioinfusion with hypertonic saline. | 2015 | 4817641 | |
| the validity of the psychoanalytic theory of paranoid dynamics. evaluated from the available experimental evidence. | 2015 | 4401621 | |
| neurologic manifestations of cardiac disease. | 2015 | 4511925 | |
| [oxidative enzyme activity in the peripheral blood leukocytes of patients with nuclear schizophrenia]. | 2015 | 4355167 | |
| studies on the preparation and properties of renin. | 2015 | 4300187 | |
| the application of magnetic resonance methods to the study of enzyme structures and action. | 2015 | 4344388 | |
| another side of the coin. | 2015 | 4405938 | |
| [disorders of calcium and phosphorus metabolism in myeloma of bone]. | 2015 | 4285276 | |
| [tarsal tunnel syndrome and subcalcaneal exostosis]. | 2015 | 4285267 | |
| giving structure to the biofilm matrix: an overview of individual strategies and emerging common themes. | biofilms are communities of microbial cells that underpin diverse processes including sewage bioremediation, plant growth promotion, chronic infections and industrial biofouling. the cells resident in the biofilm are encased within a self-produced exopolymeric matrix that commonly comprises lipids, proteins that frequently exhibit amyloid-like properties, edna and exopolysaccharides. this matrix fulfils a variety of functions for the community, from providing structural rigidity and protection f ... | 2015 | 25907113 |
| biofilm matrix proteins. | proteinaceous components of the biofilm matrix include secreted extracellular proteins, cell surface adhesins, and protein subunits of cell appendages such as flagella and pili. biofilm matrix proteins play diverse roles in biofilm formation and dissolution. they are involved in attaching cells to surfaces, stabilizing the biofilm matrix via interactions with exopolysaccharide and nucleic acid components, developing three-dimensional biofilm architectures, and dissolving biofilm matrix via enzym ... | 2015 | 26104709 |
| chromosome segregation in vibrio cholerae. | the study of chromosome segregation is currently one of the most exciting research frontiers in cell biology. in this review, we discuss our current knowledge of the chromosome segregation process in vibrio cholerae, based primarily on findings from fluorescence microscopy experiments. this bacterium is of special interest because of its eukaryotic feature of having a divided genome, a feature shared with 10% of known bacteria. we also discuss how the segregation mechanisms of v. cholerae compar ... | 2015 | 25732338 |
| the increased severity in patients presenting to hospital with diarrhea in dhaka, bangladesh since the emergence of the hybrid strain of vibrio cholerae o1 is not unique to cholera patients. | a hybrid strain of vibrio cholerae o1 el tor that expresses a classical cholera toxin (ct) emerged in 2001. this hybrid variant rapidly replaced the previous el tor strain around the world. the global emergence of this variant coincided with anecdotal reports that cholera patients were presenting with more severe dehydration and disease in many locations. | 2015 | 26409202 |
| novel gm1 ganglioside-like peptide mimics prevent the association of cholera toxin to human intestinal epithelial cells in vitro. | cholera is an acute diarrheal disease caused by infection in the gastrointestinal tract by the gram-negative bacterium, vibrio cholerae, and is a serious public health threat worldwide. there has not been any effective treatment for this infectious disease. cholera toxin (ct), which is secreted by v. cholerae, can enter host cells by binding to gm1, a monosialoganglioside widely distributed on the plasma membrane surface of various animal epithelial cells. the present study was undertaken to gen ... | 2015 | 26405107 |
| bile salts modulate the mucin-activated type vi secretion system of pandemic vibrio cholerae. | the causative agent of cholera, vibrio cholerae, regulates its diverse virulence factors to thrive in the human small intestine and environmental reservoirs. among this pathogen's arsenal of virulence factors is the tightly regulated type vi secretion system (t6ss). this system acts as an inverted bacteriophage to inject toxins into competing bacteria and eukaryotic phagocytes. v. cholerae strains responsible for the current 7th pandemic activate their t6ss within the host. we established that t ... | 2015 | 26317760 |
| genetic relatedness of selected clinical and environmental non-o1/o139 vibrio cholerae. | in an attempt to better understand the non-o1/o139 isolates of vibrio cholerae, a systematic study of clinical and environmental isolates collected from various geographical locations between the years 1932 and 1998 was conducted. | 2015 | 26164777 |
| catechol siderophore transport by vibrio cholerae. | siderophores, small iron-binding molecules secreted by many microbial species, capture environmental iron for transport back into the cell. vibrio cholerae synthesizes and uses the catechol siderophore vibriobactin and also uses siderophores secreted by other species, including enterobactin produced by escherichia coli. e. coli secretes both canonical cyclic enterobactin and linear enterobactin derivatives likely derived from its cleavage by the enterobactin esterase fes. we show here that v. ch ... | 2015 | 26100039 |
| vaccines for viral and bacterial pathogens causing acute gastroenteritis: part i: overview, vaccines for enteric viruses and vibrio cholerae. | efforts to develop vaccines for prevention of acute diarrhea have been going on for more than 40 y with partial success. the myriad of pathogens, more than 20, that have been identified as a cause of acute diarrhea throughout the years pose a significant challenge for selecting and further developing the most relevant vaccine candidates. based on pathogen distribution as identified in epidemiological studies performed mostly in low-resource countries, rotavirus, cryptosporidium, shigella, diarrh ... | 2015 | 25715048 |
| vaccines for viral and bacterial pathogens causing acute gastroenteritis: part ii: vaccines for shigella, salmonella, enterotoxigenic e. coli (etec) enterohemorragic e. coli (ehec) and campylobacter jejuni. | in part ii we discuss the following bacterial pathogens: shigella, salmonella (non-typhoidal), diarrheogenic e. coli (enterotoxigenic and enterohemorragic) and campylobacter jejuni. in contrast to the enteric viruses and vibrio cholerae discussed in part i of this series, for the bacterial pathogens described here there is only one licensed vaccine, developed primarily for vibrio cholerae and which provides moderate protection against enterotoxigenic e. coli (etec) (dukoral(®)), as well as a few ... | 2015 | 25715096 |
| unique atpase site architecture triggers cis-mediated synchronized atp binding in heptameric aaa+-atpase domain of flagellar regulatory protein flrc. | bacterial enhancer-binding proteins (bebps) oligomerize through aaa(+) domains and use atp hydrolysis-driven energy to isomerize the rna polymerase-σ(54) complex during transcriptional initiation. here, we describe the first structure of the central aaa(+) domain of the flagellar regulatory protein flrc (flrc(c)), a bebp that controls flagellar synthesis in vibrio cholerae. our results showed that flrc(c) forms heptamer both in nucleotide (nt)-free and -bound states without atp-dependent subunit ... | 2015 | 25688103 |
| differential rna-seq of vibrio cholerae identifies the vqmr small rna as a regulator of biofilm formation. | quorum sensing (qs) is a process of cell-to-cell communication that enables bacteria to transition between individual and collective lifestyles. qs controls virulence and biofilm formation in vibrio cholerae, the causative agent of cholera disease. differential rna sequencing (rna-seq) of wild-type v. cholerae and a locked low-cell-density qs-mutant strain identified 7,240 transcriptional start sites with ∼ 47% initiated in the antisense direction. a total of 107 of the transcripts do not appear ... | 2015 | 25646441 |
| multidrug efflux pumps from enterobacteriaceae, vibrio cholerae and staphylococcus aureus bacterial food pathogens. | foodborne illnesses caused by bacterial microorganisms are common worldwide and constitute a serious public health concern. in particular, microorganisms belonging to the enterobacteriaceae and vibrionaceae families of gram-negative bacteria, and to the staphylococcus genus of gram-positive bacteria are important causative agents of food poisoning and infection in the gastrointestinal tract of humans. recently, variants of these bacteria have developed resistance to medically important chemother ... | 2015 | 25635914 |
| a novel cofactor-binding mode in bacterial imp dehydrogenases explains inhibitor selectivity. | the steadily rising frequency of emerging diseases and antibiotic resistance creates an urgent need for new drugs and targets. inosine 5'-monophosphate dehydrogenase (imp dehydrogenase or impdh) is a promising target for the development of new antimicrobial agents. impdh catalyzes the oxidation of imp to xmp with the concomitant reduction of nad(+), which is the pivotal step in the biosynthesis of guanine nucleotides. potent inhibitors of bacterial impdhs have been identified that bind in a stru ... | 2015 | 25572472 |
| a cell wall damage response mediated by a sensor kinase/response regulator pair enables beta-lactam tolerance. | the bacterial cell wall is critical for maintenance of cell shape and survival. following exposure to antibiotics that target enzymes required for cell wall synthesis, bacteria typically lyse. although several cell envelope stress response systems have been well described, there is little knowledge of systems that modulate cell wall synthesis in response to cell wall damage, particularly in gram-negative bacteria. here we describe wigk/wigr, a histidine kinase/response regulator pair that enable ... | 2015 | 26712007 |
| vibrio cholerae porin ompu induces caspase-independent programmed cell death upon translocation to the host cell mitochondria. | porins, a major class of outer membrane proteins in gram-negative bacteria, primarily act as transport channels. ompu is one of the major porins of human pathogen, vibrio cholerae. in the present study, we show that v. cholerae ompu has the ability to induce target cell death. although ompu-mediated cell death shows some characteristics of apoptosis, such as flipping of phosphatidylserine in the membrane as well as cell size shrinkage and increased cell granularity, it does not show the caspase- ... | 2015 | 26559970 |
| family resemblances: a common fold for some dimeric ion-coupled secondary transporters. | membrane transporter proteins catalyze the passage of a broad range of solutes across cell membranes, allowing the uptake and efflux of crucial compounds. because of the difficulty of expressing, purifying, and crystallizing integral membrane proteins, relatively few transporter structures have been elucidated to date. although every membrane transporter has unique characteristics, structural and mechanistic similarities between evolutionarily diverse transporters have been identified. here, we ... | 2015 | 26503722 |
| crystal structure of the minor pilin cofb, the initiator of cfa/iii pilus assembly in enterotoxigenic escherichia coli. | type iv pili are extracellular polymers of the major pilin subunit. these subunits are held together in the pilus filament by hydrophobic interactions among their n-terminal α-helices, which also anchor the pilin subunits in the inner membrane prior to pilus assembly. type iv pilus assembly involves a conserved group of proteins that span the envelope of gram-negative bacteria. among these is a set of minor pilins, so named because they share their hydrophobic n-terminal polymerization/membrane ... | 2015 | 26324721 |
| predicting homogeneous pilus structure from monomeric data and sparse constraints. | type iv pili (t4p) and t2ss (type ii secretion system) pseudopili are filaments extending beyond microbial surfaces, comprising homologous subunits called "pilins." in this paper, we presented a new approach to predict pseudo atomic models of pili combining ambiguous symmetric constraints with sparse distance information obtained from experiments and based neither on electronic microscope (em) maps nor on accurate a priori symmetric details. the approach was validated by the reconstruction of th ... | 2015 | 26064954 |
| systematic identification of cyclic-di-gmp binding proteins in vibrio cholerae reveals a novel class of cyclic-di-gmp-binding atpases associated with type ii secretion systems. | cyclic-di-gmp (c-di-gmp) is a ubiquitous bacterial signaling molecule that regulates a variety of complex processes through a diverse set of c-di-gmp receptor proteins. we have utilized a systematic approach to identify c-di-gmp receptors from the pathogen vibrio cholerae using the differential radial capillary action of ligand assay (dracala). the dracala screen identified a majority of known c-di-gmp binding proteins in v. cholerae and revealed a novel c-di-gmp binding protein, mshe (vc0405), ... | 2015 | 26506097 |
| c-di-gmp regulates motile to sessile transition by modulating msha pili biogenesis and near-surface motility behavior in vibrio cholerae. | in many bacteria, including vibrio cholerae, cyclic dimeric guanosine monophosphate (c-di-gmp) controls the motile to biofilm life style switch. yet, little is known about how this occurs. in this study, we report that changes in c-di-gmp concentration impact the biosynthesis of the msha pili, resulting in altered motility and biofilm phenotypes in v. cholerae. previously, we reported that cdgj encodes a c-di-gmp phosphodiesterase and a δcdgj mutant has reduced motility and enhanced biofilm form ... | 2015 | 26505896 |
| enzymatic production of defined chitosan oligomers with a specific pattern of acetylation using a combination of chitin oligosaccharide deacetylases. | chitin and chitosan oligomers have diverse biological activities with potentially valuable applications in fields like medicine, cosmetics, or agriculture. these properties may depend not only on the degrees of polymerization and acetylation, but also on a specific pattern of acetylation (pa) that cannot be controlled when the oligomers are produced by chemical hydrolysis. to determine the influence of the pa on the biological activities, defined chitosan oligomers in sufficient amounts are need ... | 2015 | 25732514 |
| differential thiol-based switches jump-start vibrio cholerae pathogenesis. | bacterial pathogens utilize gene expression versatility to adapt to environmental changes. vibrio cholerae, the causative agent of cholera, encounters redox-potential changes when it transitions from oxygen-rich aquatic reservoirs to the oxygen-limiting human gastrointestinal tract. we previously showed that the virulence regulator aphb uses thiol-based switches to sense the anoxic host environment and transcriptionally activate the key virulence activator tcpp. here, by performing a high-throug ... | 2015 | 26748713 |
| two nucleotide second messengers regulate the production of the vibrio cholerae colonization factor gbpa. | the nucleotide second messengers camp and c-di-gmp allow many bacteria, including the human intestinal pathogen vibrio cholerae, to respond to environmental stimuli with appropriate physiological adaptations. in response to limitation of specific carbohydrates, camp and its receptor crp control the transcription of genes important for nutrient acquisition and utilization; c-di-gmp controls the transition between motile and sessile lifestyles often, but not exclusively, through transcriptional me ... | 2015 | 26286031 |
| a factor converting viable but nonculturable vibrio cholerae to a culturable state in eukaryotic cells is a human catalase. | in our previous work, we demonstrated that viable but nonculturable (vbnc) vibrio cholerae o1 and o139 were converted to culturable by coculture with eukaryotic cells. furthermore, we isolated a factor converting vbnc v. cholerae to culturable (fcvc) from a eukaryotic cell line, ht-29. in this study, we purified fcvc by successive column chromatographies comprising uno q-6 anion exchange, bio-scale cht2-1 hydroxyapatite, and superdex 200 10/300 gl. homogeneity of the purified fcvc was demonstrat ... | 2015 | 25974870 |
| the vrra srna controls a stationary phase survival factor vrp of vibrio cholerae. | small non-coding rnas (srnas) are emerging regulatory elements in bacteria. the vibrio cholerae srna vrra has previously been shown to down-regulate outer membrane proteins (ompa and ompt) and biofilm matrix protein (rbmc) by base-pairing with the 5' region of the corresponding mrnas. in this study, we present an additional target of vrra in v. cholerae, the mrna coding for the ribosome binding protein vrp. vrp is homologous to ribosome-associated inhibitor a (raia) of escherichia coli which fac ... | 2015 | 25826569 |
| identification of critical amino acids conferring lethality in vopk, a type iii effector protein of vibrio cholerae: lessons from yeast model system. | vopk, a type iii effector protein, has been implicated in the pathogenesis of vibrio cholerae strains belonging to diverse serogroups. ectopic expression of this protein exhibits strong toxicity in yeast model system. in order to map critical residues in vopk, we scanned the primary sequence guided by available data on various toxins and effector proteins. our in silico analysis of vopk indicated the presence of predicted mcf1-she (shxxxe) serine peptidase domain at the c-terminus region of the ... | 2015 | 26488395 |
| the extended regulatory networks of sxt/r391 integrative and conjugative elements and inca/c conjugative plasmids. | nowadays, healthcare systems are challenged by a major worldwide drug resistance crisis caused by the massive and rapid dissemination of antibiotic resistance genes and associated emergence of multidrug resistant pathogenic bacteria, in both clinical and environmental settings. conjugation is the main driving force of gene transfer among microorganisms. this mechanism of horizontal gene transfer mediates the translocation of large dna fragments between two bacterial cells in direct contact. inte ... | 2015 | 26347724 |
| temperature affects c-di-gmp signalling and biofilm formation in vibrio cholerae. | biofilm formation is crucial to the environmental survival and transmission of vibrio cholerae, the facultative human pathogen responsible for the disease cholera. during its infectious cycle, v. cholerae experiences fluctuations in temperature within the aquatic environment and during the transition between human host and aquatic reservoirs. in this study, we report that biofilm formation is induced at low temperatures through increased levels of the signalling molecule, cyclic diguanylate (c-d ... | 2015 | 25684220 |
| comprehensive functional analysis of the 18 vibrio cholerae n16961 toxin-antitoxin systems substantiates their role in stabilizing the superintegron. | the role of chromosomal toxin-antitoxin (ta) systems, which are ubiquitous within the genomes of free-living bacteria, is still debated. we have scanned the vibrio cholerae n16961 genome for class 2 ta genes and identified 18 gene pair candidates. interestingly, all but one are located in the chromosome 2 superintegron (si). the single ta found outside the si is located on chromosome 1 and is related to the well-characterized hipab family, which is known to play a role in antibiotic persistence. ... | 2015 | 25897030 |
| recognition of human milk oligosaccharides by bacterial exotoxins. | the affinities of the most abundant oligosaccharides found in human milk for four bacterial exotoxins (from vibrio cholerae and pathogenic escherichia coli) were quantified for the first time. association constants (ka) for a library of 20 human milk oligosaccharides (hmos) binding to shiga toxin type 2 holotoxin (stx2) and the b subunit homopentamers of cholera toxin, heat-labile toxin and shiga toxin type 1 (ctb5, hltb5 and stx1b5) were measured at 25°c and ph 7 using the direct electrospray i ... | 2015 | 25941008 |
| [the application and epidemiological research of xtag gpp multiplex pcr in the diagnosis of infectious diarrhea]. | to investigate the application value of xtag (®) gastrointestinal pathogen panel (xtag9(®) gpp) multiplex pcr in the early diagnosis of infectious diarrhea, and understand the epidemiology of intestinal diarrhea pathogens. | 2015 | 25916780 |
| inhibition of adhesion of intestinal pathogens (escherichia coli, vibrio cholerae, campylobacter jejuni, and salmonella typhimurium) by common oligosaccharides. | inhibition of the binding of pathogenic adhesins to host glycans by suitable oligosaccharides forms the basis of antiadhesion therapies. experiments were carried out to study the inhibition capability of oligosaccharides on the adhesion of four microorganisms (escherichia coli, vibrio cholerae, campylobacter jejuni, and salmonella typhimurium) to ht-29 cells. results showed that, in the absence of oligosaccharides, all of the four pathogens efficiently adhered to the cells. cell adhesion with di ... | 2015 | 25692734 |
| a comparative structure-function analysis of active-site inhibitors of vibrio cholerae cholix toxin. | cholix toxin from vibrio cholerae is a novel mono-adp-ribosyltransferase (mart) toxin that shares structural and functional properties with pseudomonas aeruginosa exotoxin a and corynebacterium diphtheriae diphtheria toxin. herein, we have used the high-resolution x-ray structure of full-length cholix toxin in the apo form, nad(+) bound, and 10 structures of the cholix catalytic domain (c-domain) complexed with several strong inhibitors of toxin enzyme activity (nap, pj34, and the p-series) to s ... | 2015 | 25756608 |
| pocket analysis of the full-length cholix toxin. an assessment of the structure-dynamics of the apo catalytic domain. | cholix toxin from vibrio cholerae is the third member of the diphtheria toxin (dt) group of mono-adp-ribosyltransferase (mart) bacterial toxins. it shares structural and functional properties with pseudomonas aeruginosa exotoxin a and corynebacterium diphtheriae dt. cholix toxin is an important model for the development of antivirulence approaches and therapeutics against these toxins from pathogenic bacteria. herein, we have used the high-resolution x-ray structure of full-length cholix complex ... | 2015 | 25559625 |
| broad spectrum anti-microbial compounds producing bacteria from coast of qingdao bays. | anti-microbial resistance burden and hazard associated with chemical treatment of infections demanded for new anti-microbial natural products. marine associated microorganisms are the enormous source of bioactive compounds. in this study we have isolated 272 marine bacteria among them 136 (50%) were antagonistic to at least one of the four pathogenic strains listeria monocytogenes, vibrio cholerae, e. coli and s. aureus. only two strains exhibited antibacterial activity against all four test str ... | 2015 | 25730803 |
| poly(amidoamine) dendrimers show carbonic anhydrase inhibitory activity against α-, β-, γ- and η-class enzymes. | four generations of poly(amidoamine) (pamam) dendrimers incorporating benzenesulfonamide moieties were investigated as inhibitors of carbonic anhydrases (cas, ec 4.2.1.1) belonging to the α-, β-, γ- and η-classes which are present in pathogenic bacteria, fungi or protozoa. the following bacterial, fungal and protozoan organisms were included in the study: vibrio cholerae, trypanosoma cruzi, leishmania donovani chagasi, porphyromonas gingivalis, cryptococcus neoformans, candida glabrata, and plas ... | 2015 | 26476668 |
| graphene oxide nanoribbon-based sensors for the simultaneous bio-electrochemical enantiomeric resolution and analysis of amino acid biomarkers. | in this work, a straightforward in-situ measurement of l and d-amino acids (aas) has been developed using disposable graphene oxide nanoribbon (gon) screen printed electrodes. for that, we took advantage of the electroactivity of certain clinically relevant aas, such as tyrosine (tyr) and methionine (met), which are involved in important bacterial diseases (bacillus subtilis and vibrio cholera, respectively). the strategy is based on a dual electrochemical and enzymatic approach. the d-aa with t ... | 2015 | 25562744 |
| dbdiasnp: an open-source knowledgebase of genetic polymorphisms and resistance genes related to diarrheal pathogens. | diarrhea is a highly common infection among children, responsible for significant morbidity and mortality rate worldwide. after pneumonia, diarrhea remains the second leading cause of neonatal deaths. numerous viral, bacterial, and parasitic enteric pathogens are associated with diarrhea. with increasing antibiotic resistance among enteric pathogens, there is an urgent need for global surveillance of the mutations and resistance genes primarily responsible for resistance to antibiotic treatment. ... | 2015 | 25978092 |
| comparative evaluation of the protective efficacy of two formulations of a recombinant chlamydia abortus subunit candidate vaccine in a mouse model. | chlamydia abortus (c. abortus) is the causative agent of ovine enzootic abortion (oea) and poses a zoonotic risk to pregnant women. current live attenuated 1b vaccines are efficacious but cause disease in vaccinated animals and inactivated vaccines are only marginally protective. we tested the ability of a new c. abortus subunit vaccine candidate based on the conserved and immunogenic polymorphic membrane protein d (pmp18d) formulated in cpg1826+fl (fms-like tyrosine kinase 3 ligand; flt3l) or v ... | 2015 | 25698486 |
| crp-dependent positive autoregulation and proteolytic degradation regulate competence activator sxy of escherichia coli. | natural competence, the ability of bacteria to take up exogenous dna and incorporate it into their chromosomes, is in most bacteria a transient phenomenon under complex genetic and environmental control. in the gram-negative bacteria haemophilus influenzae and vibrio cholerae, the master regulator sxy/tfox controls competence development. although not known to be naturally competent, escherichia coli possesses a sxy homologue and a competence regulon containing the genes required for dna uptake. ... | 2015 | 25491382 |
| antibiotic-resistant bacteria: prevalence in food and inactivation by food-compatible compounds and plant extracts. | foodborne antibiotic-resistant pathogenic bacteria such as campylobacter jejuni, bacillus cereus, clostridium perfringens, escherichia coli, salmonella enterica, staphylococcus aureus, vibrio cholerae, and vibrio parahemolyticus can adversely affect animal and human health, but a better understanding of the factors involved in their pathogenesis is needed. to help meet this need, this overview surveys and interprets much of our current knowledge of antibiotic (multidrug)-resistant bacteria in th ... | 2015 | 25856120 |
| tracking the elusive function of bacillus subtilis hfq. | rna-binding protein hfq is a key component of the adaptive responses of many proteobacterial species including escherichia coli, salmonella enterica and vibrio cholera. in these organisms, the importance of hfq largely stems from its participation to regulatory mechanisms involving small non-coding rnas. in contrast, the function of hfq in gram-positive bacteria has remained elusive and somewhat controversial. in the present study, we have further addressed this point by comparing growth phenoty ... | 2015 | 25915524 |
| two conserved amino acids of juxtaposed domains of a ribosomal maturation protein cgta sustain its optimal gtpase activity. | cgta is a highly conserved ribosome binding protein involved in ribosome biogenesis and associated with stringent response. it is a 55 kda gtpase protein consisting of gtpase, obg and c-terminal domains. the function of the latter two domains was not clear and despite the importance, the mode of action of cgta is still largely unknown. knocking out of cgta gene is lethal and mutations lead to growth, sporulation and developmental defects in bacteria. it was found that a growth defect and pinhole ... | 2015 | 25912137 |
| dual inducer signal recognition by an mlc homologue. | the mlc transcription factor in escherichia coli controls the expression of the phosphotransferase system genes implicated in the transport of glucose into the cell. transport of glucose derepresses mlc-repressed genes by provoking the sequestration of mlc to the membrane, via an interaction with the dephosphorylated eiib domain of the glucose transporter, ptsg. nagc, a paralogue of mlc in e. coli, regulates the use of the amino sugar n-acetylglucosamine (glcnac). both mlc and nagc are members o ... | 2015 | 26293172 |
| molecular dissection of the essential features of the origin of replication of the second vibrio cholerae chromosome. | vibrionaceae family members are interesting models for studying dna replication initiation, as they contain two circular chromosomes. chromosome ii (chrii) replication is governed by two evolutionarily unique yet highly conserved elements, the origin dna sequence oricii and the initiator protein rctb. the minimum functional region of oricii, oricii-min, contains multiple elements that are bound by rctb in vitro, but little is known about the specific requirements for individual elements during o ... | 2015 | 26220967 |
| the roles of inflammation, nutrient availability and the commensal microbiota in enteric pathogen infection. | the healthy human intestine is colonized by as many as 1014 bacteria belonging to more than 500 different species forming a microbial ecosystem of unsurpassed diversity, termed the microbiota. the microbiota's various bacterial members engage in a physiological network of cooperation and competition within several layers of complexity. within the last 10 years, technological progress in the field of next-generation sequencing technologies has tremendously advanced our understanding of the wide v ... | 2015 | 26185088 |
| a conserved pattern of primer-dependent transcription initiation in escherichia coli and vibrio cholerae revealed by 5' rna-seq. | transcription initiation that involves the use of a 2- to ~4-nt oligoribonucleotide primer, "primer-dependent initiation," (pdi) has been shown to be widely prevalent at promoters of genes expressed during the stationary phase of growth in escherichia coli. however, the extent to which pdi impacts e. coli physiology, and the extent to which pdi occurs in other bacteria is not known. here we establish a physiological role for pdi in e. coli as a regulatory mechanism that modulates biofilm formati ... | 2015 | 26131907 |
| established microbial colonies can survive type vi secretion assault. | type vi secretion (t6s) is a cell-to-cell injection system that can be used as a microbial weapon. t6s kills vulnerable cells, and is present in close to 25% of sequenced gram-negative bacteria. to examine the ecological role of t6s among bacteria, we competed self-immune t6s+ cells and t6s-sensitive cells in simulated range expansions. as killing takes place only at the interface between sensitive and t6s+ strains, while growth takes place everywhere, sufficiently large domains of sensitive cel ... | 2015 | 26485125 |
| attributes of carbapenemase encoding conjugative plasmid pndm-sal from an extensively drug-resistant salmonella enterica serovar senftenberg. | a carbapenem resistant salmonella enterica serovar senftenberg isolate bch 2406 was isolated from a diarrheal child attending an outpatient unit of b.c. roy hospital in kolkata, india. this isolate was positive for the bla ndm-1 in the pcr assay, which was confirmed by amplicon sequencing. except for tetracycline, this isolate was resistant to all the tested antimicrobials. the bla ndm-1 was found to be located on a 146.13-kb mega plasmid pndm-sal, which could be conjugally transferred into esch ... | 2015 | 26441902 |
| antibacterial efficacy of five medicinal plants against multidrug-resistant enteropathogenic bacteria infecting under-5 hospitalized children. | to evaluate in vitro antibacterial effectiveness of five medicinal plants used by an indian aborigine, against 8 multidrug-resistant (mdr) enteropathogenic bacteria isolated from clinical samples of under-5 hospitalized children. | 2015 | 25609371 |
| biosynthesis, characterisation and antimicrobial activity of silver nanoparticles using hibiscus rosa-sinensis petals extracts. | green synthesis of metallic nanoparticles has lured the world from the chemical and physical approaches owing to its rapid, non-hazardous and economic aspect of production mechanism. in this study, silver nanoparticles (agnps) were synthesised using petal extracts of hibiscus rosa-sinensis. the agnps displayed characteristic surface plasmon resonance peak at around 421 nm having a mean particle size of 76.25±0.17 nm and carried a charge of -41±0.2 mv. the x-ray diffraction patterns displayed typ ... | 2015 | 26435282 |
| a dye-decolorizing peroxidase from vibrio cholerae. | the dye-decolorizing peroxidase (dyp) protein from vibrio cholerae (vcdyp) was expressed in escherichia coli, and its dyp activity was assayed by monitoring degradation of a typical anthraquinone dye, reactive blue 19 (rb19). its kinetic activity was obtained by fitting the data to the michaelis-menten equation, giving kcat and km values of 1.3 ± 0.3 s(-1) and 50 ± 20 μm, respectively, which are comparable to those of other dyp enzymes. the enzymatic activity of vcdyp was highest at ph 4. a muta ... | 2015 | 26431465 |
| prediction of intra-species protein-protein interactions in enteropathogens facilitating systems biology study. | protein-protein interactions in escherichia coli (e. coli) has been studied extensively using high throughput methods such as tandem affinity purification followed by mass spectrometry and yeast two-hybrid method. this can in turn be used to understand the mechanisms of bacterial cellular processes. however, experimental characterization of such huge amount of interactions data is not available for other important enteropathogens. here, we propose a support vector machine (svm)-based prediction ... | 2015 | 26717407 |
| secretome analysis of vibrio cholerae type vi secretion system reveals a new effector-immunity pair. | the type vi secretion system (t6ss) is a dynamic macromolecular organelle that many gram-negative bacteria use to inhibit or kill other prokaryotic or eukaryotic cells. the toxic effectors of t6ss are delivered to the prey cells in a contact-dependent manner. in vibrio cholerae, the etiologic agent of cholera, t6ss is active during intestinal infection. here, we describe the use of comparative proteomics coupled with bioinformatics to identify a new t6ss effector-immunity pair. this analysis was ... | 2015 | 25759499 |
| hygiene and health risks associated with the consumption of edible lamellibranch molluscs. | edible lamellibranch molluscs are frequently the cause of very serious outbreaks of food poisoning. microbiological suitability for human consumption, according to ec reg. n. 1441/2007, is only based on two faecal bacteriological contamination parameters (escherichia coli and salmonella) and does not consider other microorganisms which are naturally present within the marine environment and are potentially pathogenic such as those belonging to the genus vibrio. in this study, a total of 540 samp ... | 2015 | 25744696 |
| antimicrobial activity of the essential oil from the leaves and seeds of coriandrum sativum toward food-borne pathogens. | the increasing incidence of drug-resistant pathogens and toxicity of existing antibacterial compounds has drawn attention toward the antimicrobial activity of natural products. the purpose of this study is to evaluate the antimicrobial activity of the essential oil of the leaves and seeds of coriandrum sativum. the five strains of bacteria comprising escherichia coli, staphylococcus aureus, yersinia enterocolitica, salmonella enterica and vibrio cholerae were used for the antibacterial tests. in ... | 2015 | 26684163 |
| crystal structure and kinetic studies of a tetrameric type ii β-carbonic anhydrase from the pathogenic bacterium vibrio cholerae. | carbonic anhydrase (ca) is a zinc enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate (hydrogen carbonate) and a proton. cas have been extensively investigated owing to their involvement in numerous physiological and pathological processes. currently, ca inhibitors are widely used as antiglaucoma, anticancer and anti-obesity drugs and for the treatment of neurological disorders. recently, the potential use of ca inhibitors to fight infections caused by protozoa, fung ... | 2015 | 26627652 |
| vibrio spp. from macrobrachium amazonicum prawn farming are inhibited by moringa oleifera extracts. | to investigate the in vitro antimicrobial potential of extracts of stem, leaves, flowers, pods and seeds of moringa oleifera (m. oleifera) against vibrio spp. from hatchery water and the prawn macrobrachium amazonicum. | 2015 | 26614991 |
| survival of enteroaggregative escherichia coli and vibrio cholerae in frozen and chilled foods. | enteroaggregative escherichia coli (eaec) and vibrio cholerae are common bacteria that infect people in developing countries. nowadays, food preservation by freezing and refrigeration are very common practices that extend the shelf life of food products. unfortunately, eaec and v. cholerae are suspected to survive at low temperatures. | 2015 | 26322875 |
| crystal structure and activity of protein l-isoaspartyl-o-methyltransferase from vibrio cholerae, and the effect of adohcy binding. | the repair enzyme protein l-isoaspartyl-o-methyltransferase (pimt) is widely distributed in various organisms. pimt catalyzes s-adenosylmethionine (adomet) dependent methylation of abnormal l-isoaspartyl residues, formed by the deamidation of asparagines and isomerization of aspartates. we report the crystal structure of pimt of vibrio cholerae (vcpimt), the aetiological agent for cholera, complexed with the demethylated cofactor s-adenosyl-l-homocysteine (adohcy) to 2.05 å resolution. a stretch ... | 2015 | 26255776 |
| functional relbe-family toxin-antitoxin pairs affect biofilm maturation and intestine colonization in vibrio cholerae. | toxin-antitoxin (ta) systems are small genetic elements that typically encode a stable toxin and its labile antitoxin. these cognate pairs are abundant in prokaryotes and have been shown to regulate various cellular functions. vibrio cholerae, a human pathogen that is the causative agent of cholera, harbors at least thirteen ta loci. while functional higba, parde have been shown to stabilize plasmids and phd/doc to mediate cell death in v. cholerae, the function of seven relbe-family ta systems ... | 2015 | 26275048 |
| surveillance for foodborne disease outbreaks in iran, 2006-2011. | the outbreaks of foodborne diseases is a major health problem and occur daily in all countries, from the most to the least developed. this study is the first report of foodborne outbreaks in iran that carried out from 2006 to 2011. | 2015 | 26913248 |
| response of vibrio cholerae to the catecholamine hormones epinephrine and norepinephrine. | in escherichia coli or salmonella enterica, the stress-associated mammalian hormones epinephrine (e) and norepinephrine (ne) trigger a signaling cascade by interacting with the qsec sensor protein. here we show that vibrio cholerae, the causative agent of cholera, exhibits a specific response to e and ne. these catecholates (0.1 mm) enhanced the growth and swimming motility of v. cholerae strain o395 on soft agar in a medium containing calf serum, which simulated the environment within the host. ... | 2015 | 26416829 |
| epidemiologic and drug resistance pattern of vibrio cholerae o1 biotype el tor, serotype ogawa, in the 2011 cholera outbreak, in alborz province, iran. | although the national guidelines recommend special antibiotics, based on the antibiogram of national reference laboratory, it seems that, because of uncontrolled usage of antibiotics in the society and due to the changes in the serotypes causing the disease, it is essential to monitor the status of drug resistance, permanently, and to revise the current prescriptions guidelines. | 2015 | 26855737 |