Publications

studies on the preparation and properties of renin.		2015	4300187
the application of magnetic resonance methods to the study of enzyme structures and action.		2015	4344388
another side of the coin.		2015	4405938
[disorders of calcium and phosphorus metabolism in myeloma of bone].		2015	4285276
[tarsal tunnel syndrome and subcalcaneal exostosis].		2015	4285267
echography of mixed tumours of the lacrimal gland.		2015	4700137
echography of mixed tumours of the lacrimal gland.		2015	4700137
chylopericardium and chylothorax.		2015	4704244
chylopericardium and chylothorax.		2015	4704244
[early changes in thoracic radiographs following surgical operations of the thorax].		2015	4569734
some hemodynamic effects of sodium nitroprusside.		2015	4567618
romanian contributions to the progress in hematology.		2015	4572550
centric relation records: the effect of muscle action.		2015	4521343
frank discussions says cdnaa president.		2015	4522760
raynaud's disorders revisited.		2015	4843173
[study of vestibular function after stapedectomy].		2015	4394237
a psychoendocrine study of bereavement. ii. observations on the process of mourning in relation to adrenocortical function.		2015	4644658
the treatment of obesity.		2015	4573726
[monitoring cellular immunity in patients following renal transplantation].		2015	4566253
evaluation of drug history program.		2015	4718727
complete recovery after resuscitation and emergency craniotomy in three children with acute epidural haematoma.		2015	4653747
[preoperative clinical characteristics in patients with ulcerative colitis submitted to surgical treatment].		2015	4829349
[cerebral gigantism].		2015	4431171
[administration of corticosteroids to patients with hormone-producing tumors of the adrenal cortex].		2015	4457702
the pensacola story.		2015	4551267
[prognosis in leprosy].		2015	4480378
[massive doses of thiamine in neurosurgical resuscitation].		2015	4690563
antifertility agents: introduction and history.	the article is a brief historical account of the birth and development of steroidal contraception. over 400 oral contraceptive agents have been and/or are in use. attention is called to particular medical writers who have composed excellent historical accounts of the development of the synthetic estrogens and progestins used in commercial and investigative oral contraceptives.	2015	4584283
growth hormone levels in slipped capital femoral epiphysis.		2015	4652052
[decubitus ulcer and nursing--observations on the etiology of decubitus ulcer in the neurosurgery department].		2015	4482651
[hazards of electrical equipment].		2015	4483657
[neonatal tuberculosis. discussion of 2 cases].		2015	4546632
[homo-transplantation of a tooth].		2015	4527387
pigmented fungiform papillae of the tongue: a common variant of oral pigmentation.		2015	4430531
[development of the cottbus school for dental technicians].		2015	4514183
cholesterol and bile salt studies on the bile of patients with cholesterol gallstones.	gallbladder bile from patients with cholesterol stones and control patients without cholesterol stones was analysed for the relative concentrations of bile salts, phospholipids, and cholesterol. it was not possible to distinguish stone-forming from control biles when these were plotted on triangular coordinates. there was a significant increase in the dihydroxy: trihydroxy bile salt ratio in the patients with cholesterol gallstones. the maximum cholesterol holding capacity for the bile salts was ...	2015	4696536
ultrafilter membranes in biochemistry.		2015	4612299
behavioral studies of noise-induced hearing loss in primates: loudness recruitment.		2015	4351038
the treatment of congenital heart disease in infants. surface cooling, deep hypothermia, limited cardiopulmonary bypass and circulatory arrest.		2015	4693334
[clinical study on posttraumatic sequelae of the brain, with special reference to the analysis of cases treated in the department of surgery].		2015	4288859
[advanced training in nursing. iv. education of departmental nurses and nurses' aides].		2015	4481236
identification of the target dna sequence and characterization of dna binding features of hlyu, and suggestion of a redox switch for hlya expression in the human pathogen vibrio cholerae from in silico studies.	hlyu, a transcriptional regulator common in many vibrio species, activates the hemolysin gene hlya in vibrio cholerae, the rtxa1 operon in vibrio vulnificus and the genes of plp-vah1 and rtxachbde gene clusters in vibrio anguillarum. the protein is also proposed to be a potential global virulence regulator for v. cholerae and v. vulnificus. mechanisms of gene control by hlyu in v. vulnificus and v. anguillarum are reported. however, detailed elucidation of the interaction of hlyu in v. cholerae ...	2015	25605793
enzymatic production of defined chitosan oligomers with a specific pattern of acetylation using a combination of chitin oligosaccharide deacetylases.	chitin and chitosan oligomers have diverse biological activities with potentially valuable applications in fields like medicine, cosmetics, or agriculture. these properties may depend not only on the degrees of polymerization and acetylation, but also on a specific pattern of acetylation (pa) that cannot be controlled when the oligomers are produced by chemical hydrolysis. to determine the influence of the pa on the biological activities, defined chitosan oligomers in sufficient amounts are need ...	2015	25732514
autophagy and endosomal trafficking inhibition by vibrio cholerae martx toxin phosphatidylinositol-3-phosphate-specific phospholipase a1 activity.	vibrio cholerae, responsible for acute gastroenteritis secretes a large multifunctional-autoprocessing repeat-in-toxin (martx) toxin linked to evasion of host immune system, facilitating colonization of small intestine. unlike other effector domains of the multifunctional toxin that target cytoskeleton, the function of alpha-beta hydrolase (abh) remained elusive. this study demonstrates that abh is an esterase/lipase with catalytic ser-his-asp triad. abh binds with high affinity to phosphatidyli ...	2015	26498860
an intracellular replication niche for vibrio cholerae in the amoeba acanthamoeba castellanii.	vibrio cholerae is a human pathogen and the causative agent of cholera. the persistence of this bacterium in aquatic environments is a key epidemiological concern, as cholera is transmitted through contaminated water. predatory protists, such as amoebae, are major regulators of bacterial populations in such environments. therefore, we investigated the interaction between v. cholerae and the amoeba acanthamoeba castellanii at the single-cell level. we observed that v. cholerae can resist intracel ...	2015	26394005
the cpx system regulates virulence gene expression in vibrio cholerae.	bacteria possess signal transduction pathways capable of sensing and responding to a wide variety of signals. the cpx envelope stress response, composed of the sensor histidine kinase cpxa and the response regulator cpxr, senses and mediates adaptation to insults to the bacterial envelope. the cpx response has been implicated in the regulation of a number of envelope-localized virulence determinants across bacterial species. here, we show that activation of the cpx pathway in vibrio cholerae el ...	2015	25824837
a factor converting viable but nonculturable vibrio cholerae to a culturable state in eukaryotic cells is a human catalase.	in our previous work, we demonstrated that viable but nonculturable (vbnc) vibrio cholerae o1 and o139 were converted to culturable by coculture with eukaryotic cells. furthermore, we isolated a factor converting vbnc v. cholerae to culturable (fcvc) from a eukaryotic cell line, ht-29. in this study, we purified fcvc by successive column chromatographies comprising uno q-6 anion exchange, bio-scale cht2-1 hydroxyapatite, and superdex 200 10/300 gl. homogeneity of the purified fcvc was demonstrat ...	2015	25974870
differential thiol-based switches jump-start vibrio cholerae pathogenesis.	bacterial pathogens utilize gene expression versatility to adapt to environmental changes. vibrio cholerae, the causative agent of cholera, encounters redox-potential changes when it transitions from oxygen-rich aquatic reservoirs to the oxygen-limiting human gastrointestinal tract. we previously showed that the virulence regulator aphb uses thiol-based switches to sense the anoxic host environment and transcriptionally activate the key virulence activator tcpp. here, by performing a high-throug ...	2015	26748713
systematic identification of cyclic-di-gmp binding proteins in vibrio cholerae reveals a novel class of cyclic-di-gmp-binding atpases associated with type ii secretion systems.	cyclic-di-gmp (c-di-gmp) is a ubiquitous bacterial signaling molecule that regulates a variety of complex processes through a diverse set of c-di-gmp receptor proteins. we have utilized a systematic approach to identify c-di-gmp receptors from the pathogen vibrio cholerae using the differential radial capillary action of ligand assay (dracala). the dracala screen identified a majority of known c-di-gmp binding proteins in v. cholerae and revealed a novel c-di-gmp binding protein, mshe (vc0405), ...	2015	26506097
two nucleotide second messengers regulate the production of the vibrio cholerae colonization factor gbpa.	the nucleotide second messengers camp and c-di-gmp allow many bacteria, including the human intestinal pathogen vibrio cholerae, to respond to environmental stimuli with appropriate physiological adaptations. in response to limitation of specific carbohydrates, camp and its receptor crp control the transcription of genes important for nutrient acquisition and utilization; c-di-gmp controls the transition between motile and sessile lifestyles often, but not exclusively, through transcriptional me ...	2015	26286031
identification of critical amino acids conferring lethality in vopk, a type iii effector protein of vibrio cholerae: lessons from yeast model system.	vopk, a type iii effector protein, has been implicated in the pathogenesis of vibrio cholerae strains belonging to diverse serogroups. ectopic expression of this protein exhibits strong toxicity in yeast model system. in order to map critical residues in vopk, we scanned the primary sequence guided by available data on various toxins and effector proteins. our in silico analysis of vopk indicated the presence of predicted mcf1-she (shxxxe) serine peptidase domain at the c-terminus region of the ...	2015	26488395
vibrio cholerae csra regulates toxr levels in response to amino acids and is essential for virulence.	toxr is a major virulence gene regulator in vibrio cholerae. although constitutively expressed under many laboratory conditions, our previous work demonstrated that the level of toxr increases significantly when cells are grown in the presence of the 4 amino acids asparagine, arginine, glutamate, and serine (nres). we show here that the increase in toxr production in response to nres requires the var/csr global regulatory circuit. the vars/vara two-component system controls the amount of active ...	2015	26242626
in situ proteolysis of the vibrio cholerae matrix protein rbma promotes biofilm recruitment.	the estuarine gram-negative rod and human diarrheal pathogen vibrio cholerae synthesizes a vps exopolysaccharide-dependent biofilm matrix that allows it to form a 3d structure on surfaces. proteins associated with the matrix include, rbma, rbmc, and bap1. rbma, a protein whose crystallographic structure suggests two binding surfaces, associates with cells by means of a vps-dependent mechanism and promotes biofilm cohesiveness and recruitment of cells to the biofilm. here, we show that rbma under ...	2015	26240338
quorum sensing-regulated chitin metabolism provides grazing resistance to vibrio cholerae biofilms.	association of vibrio cholerae with chitinous surfaces of zooplankton is important for its persistence in marine environments, as it provides accessibility to nutrients and resistance to stresses. predation by heterotrophic protists has a major impact on the survival of v. cholerae. v. cholerae forms biofilms as its main defensive strategy, and quorum sensing (qs) additionally regulates the production of antiprotozoal factors. the role of chitin and qs regulation in v. cholerae grazing resistanc ...	2015	25615438
c-di-gmp regulates motile to sessile transition by modulating msha pili biogenesis and near-surface motility behavior in vibrio cholerae.	in many bacteria, including vibrio cholerae, cyclic dimeric guanosine monophosphate (c-di-gmp) controls the motile to biofilm life style switch. yet, little is known about how this occurs. in this study, we report that changes in c-di-gmp concentration impact the biosynthesis of the msha pili, resulting in altered motility and biofilm phenotypes in v. cholerae. previously, we reported that cdgj encodes a c-di-gmp phosphodiesterase and a δcdgj mutant has reduced motility and enhanced biofilm form ...	2015	26505896
the vrra srna controls a stationary phase survival factor vrp of vibrio cholerae.	small non-coding rnas (srnas) are emerging regulatory elements in bacteria. the vibrio cholerae srna vrra has previously been shown to down-regulate outer membrane proteins (ompa and ompt) and biofilm matrix protein (rbmc) by base-pairing with the 5' region of the corresponding mrnas. in this study, we present an additional target of vrra in v. cholerae, the mrna coding for the ribosome binding protein vrp. vrp is homologous to ribosome-associated inhibitor a (raia) of escherichia coli which fac ...	2015	25826569
the increased severity in patients presenting to hospital with diarrhea in dhaka, bangladesh since the emergence of the hybrid strain of vibrio cholerae o1 is not unique to cholera patients.	a hybrid strain of vibrio cholerae o1 el tor that expresses a classical cholera toxin (ct) emerged in 2001. this hybrid variant rapidly replaced the previous el tor strain around the world. the global emergence of this variant coincided with anecdotal reports that cholera patients were presenting with more severe dehydration and disease in many locations.	2015	26409202
novel gm1 ganglioside-like peptide mimics prevent the association of cholera toxin to human intestinal epithelial cells in vitro.	cholera is an acute diarrheal disease caused by infection in the gastrointestinal tract by the gram-negative bacterium, vibrio cholerae, and is a serious public health threat worldwide. there has not been any effective treatment for this infectious disease. cholera toxin (ct), which is secreted by v. cholerae, can enter host cells by binding to gm1, a monosialoganglioside widely distributed on the plasma membrane surface of various animal epithelial cells. the present study was undertaken to gen ...	2015	26405107
bile salts modulate the mucin-activated type vi secretion system of pandemic vibrio cholerae.	the causative agent of cholera, vibrio cholerae, regulates its diverse virulence factors to thrive in the human small intestine and environmental reservoirs. among this pathogen's arsenal of virulence factors is the tightly regulated type vi secretion system (t6ss). this system acts as an inverted bacteriophage to inject toxins into competing bacteria and eukaryotic phagocytes. v. cholerae strains responsible for the current 7th pandemic activate their t6ss within the host. we established that t ...	2015	26317760
genetic relatedness of selected clinical and environmental non-o1/o139 vibrio cholerae.	in an attempt to better understand the non-o1/o139 isolates of vibrio cholerae, a systematic study of clinical and environmental isolates collected from various geographical locations between the years 1932 and 1998 was conducted.	2015	26164777
catechol siderophore transport by vibrio cholerae.	siderophores, small iron-binding molecules secreted by many microbial species, capture environmental iron for transport back into the cell. vibrio cholerae synthesizes and uses the catechol siderophore vibriobactin and also uses siderophores secreted by other species, including enterobactin produced by escherichia coli. e. coli secretes both canonical cyclic enterobactin and linear enterobactin derivatives likely derived from its cleavage by the enterobactin esterase fes. we show here that v. ch ...	2015	26100039
vaccines for viral and bacterial pathogens causing acute gastroenteritis: part i: overview, vaccines for enteric viruses and vibrio cholerae.	efforts to develop vaccines for prevention of acute diarrhea have been going on for more than 40 y with partial success. the myriad of pathogens, more than 20, that have been identified as a cause of acute diarrhea throughout the years pose a significant challenge for selecting and further developing the most relevant vaccine candidates. based on pathogen distribution as identified in epidemiological studies performed mostly in low-resource countries, rotavirus, cryptosporidium, shigella, diarrh ...	2015	25715048
vaccines for viral and bacterial pathogens causing acute gastroenteritis: part ii: vaccines for shigella, salmonella, enterotoxigenic e. coli (etec) enterohemorragic e. coli (ehec) and campylobacter jejuni.	in part ii we discuss the following bacterial pathogens: shigella, salmonella (non-typhoidal), diarrheogenic e. coli (enterotoxigenic and enterohemorragic) and campylobacter jejuni. in contrast to the enteric viruses and vibrio cholerae discussed in part i of this series, for the bacterial pathogens described here there is only one licensed vaccine, developed primarily for vibrio cholerae and which provides moderate protection against enterotoxigenic e. coli (etec) (dukoral(®)), as well as a few ...	2015	25715096
unique atpase site architecture triggers cis-mediated synchronized atp binding in heptameric aaa+-atpase domain of flagellar regulatory protein flrc.	bacterial enhancer-binding proteins (bebps) oligomerize through aaa(+) domains and use atp hydrolysis-driven energy to isomerize the rna polymerase-σ(54) complex during transcriptional initiation. here, we describe the first structure of the central aaa(+) domain of the flagellar regulatory protein flrc (flrc(c)), a bebp that controls flagellar synthesis in vibrio cholerae. our results showed that flrc(c) forms heptamer both in nucleotide (nt)-free and -bound states without atp-dependent subunit ...	2015	25688103
differential rna-seq of vibrio cholerae identifies the vqmr small rna as a regulator of biofilm formation.	quorum sensing (qs) is a process of cell-to-cell communication that enables bacteria to transition between individual and collective lifestyles. qs controls virulence and biofilm formation in vibrio cholerae, the causative agent of cholera disease. differential rna sequencing (rna-seq) of wild-type v. cholerae and a locked low-cell-density qs-mutant strain identified 7,240 transcriptional start sites with ∼ 47% initiated in the antisense direction. a total of 107 of the transcripts do not appear ...	2015	25646441
multidrug efflux pumps from enterobacteriaceae, vibrio cholerae and staphylococcus aureus bacterial food pathogens.	foodborne illnesses caused by bacterial microorganisms are common worldwide and constitute a serious public health concern. in particular, microorganisms belonging to the enterobacteriaceae and vibrionaceae families of gram-negative bacteria, and to the staphylococcus genus of gram-positive bacteria are important causative agents of food poisoning and infection in the gastrointestinal tract of humans. recently, variants of these bacteria have developed resistance to medically important chemother ...	2015	25635914
a novel cofactor-binding mode in bacterial imp dehydrogenases explains inhibitor selectivity.	the steadily rising frequency of emerging diseases and antibiotic resistance creates an urgent need for new drugs and targets. inosine 5'-monophosphate dehydrogenase (imp dehydrogenase or impdh) is a promising target for the development of new antimicrobial agents. impdh catalyzes the oxidation of imp to xmp with the concomitant reduction of nad(+), which is the pivotal step in the biosynthesis of guanine nucleotides. potent inhibitors of bacterial impdhs have been identified that bind in a stru ...	2015	25572472
giving structure to the biofilm matrix: an overview of individual strategies and emerging common themes.	biofilms are communities of microbial cells that underpin diverse processes including sewage bioremediation, plant growth promotion, chronic infections and industrial biofouling. the cells resident in the biofilm are encased within a self-produced exopolymeric matrix that commonly comprises lipids, proteins that frequently exhibit amyloid-like properties, edna and exopolysaccharides. this matrix fulfils a variety of functions for the community, from providing structural rigidity and protection f ...	2015	25907113
biofilm matrix proteins.	proteinaceous components of the biofilm matrix include secreted extracellular proteins, cell surface adhesins, and protein subunits of cell appendages such as flagella and pili. biofilm matrix proteins play diverse roles in biofilm formation and dissolution. they are involved in attaching cells to surfaces, stabilizing the biofilm matrix via interactions with exopolysaccharide and nucleic acid components, developing three-dimensional biofilm architectures, and dissolving biofilm matrix via enzym ...	2015	26104709
chromosome segregation in vibrio cholerae.	the study of chromosome segregation is currently one of the most exciting research frontiers in cell biology. in this review, we discuss our current knowledge of the chromosome segregation process in vibrio cholerae, based primarily on findings from fluorescence microscopy experiments. this bacterium is of special interest because of its eukaryotic feature of having a divided genome, a feature shared with 10% of known bacteria. we also discuss how the segregation mechanisms of v. cholerae compar ...	2015	25732338
the extended regulatory networks of sxt/r391 integrative and conjugative elements and inca/c conjugative plasmids.	nowadays, healthcare systems are challenged by a major worldwide drug resistance crisis caused by the massive and rapid dissemination of antibiotic resistance genes and associated emergence of multidrug resistant pathogenic bacteria, in both clinical and environmental settings. conjugation is the main driving force of gene transfer among microorganisms. this mechanism of horizontal gene transfer mediates the translocation of large dna fragments between two bacterial cells in direct contact. inte ...	2015	26347724
temperature affects c-di-gmp signalling and biofilm formation in vibrio cholerae.	biofilm formation is crucial to the environmental survival and transmission of vibrio cholerae, the facultative human pathogen responsible for the disease cholera. during its infectious cycle, v. cholerae experiences fluctuations in temperature within the aquatic environment and during the transition between human host and aquatic reservoirs. in this study, we report that biofilm formation is induced at low temperatures through increased levels of the signalling molecule, cyclic diguanylate (c-d ...	2015	25684220
comprehensive functional analysis of the 18 vibrio cholerae n16961 toxin-antitoxin systems substantiates their role in stabilizing the superintegron.	the role of chromosomal toxin-antitoxin (ta) systems, which are ubiquitous within the genomes of free-living bacteria, is still debated. we have scanned the vibrio cholerae n16961 genome for class 2 ta genes and identified 18 gene pair candidates. interestingly, all but one are located in the chromosome 2 superintegron (si). the single ta found outside the si is located on chromosome 1 and is related to the well-characterized hipab family, which is known to play a role in antibiotic persistence. ...	2015	25897030
[the activity of factor xiii in plasma of children with acute leucaemia (author's transl)].		2015	4518176
effectiveness and efficiency in therapy.		2015	4690387
[caucasian period of the activity of v. s. bobrovskiĭ (on the 100th anniversary of his birth)].		2015	4592872
[bleeding in oral surgery].		2015	4542263
[total ligation of the esophagus by murphy's button. hemmorrhages caused by esophageal varices in portal hypertension. apropos of 34 cases].		2015	4549250
observations on pulmonary function testing and blood-gas analysis in children.		2015	4503927
a2 cell pancreatic microadenomas in a case of multiple endocrine adenomatosis.		2015	4398556
the role of immunofluorescence in the diagnosis of oral vesiculobullous disorders. report of four cases.		2015	4551232
[ethics in dental practice].		2015	4506460
sewage treatment by means of oxidation ponds.		2015	4432066
diagnostic abdominal radioisotopic lymphography and radiation therapy.		2015	4333860
[ischemic processes involving the hands].		2015	4440915
[testicular metastasis of a muciparous gastric epithelioma].		2015	4290981
prevalence and antimicrobial resistance of vibrio spp. in retail and farm shrimps in ecuador.	the aim of this study was to investigate the prevalence of vibrio spp. in shrimp at retail and in shrimp farms in ecuador and to determine the antimicrobial agent resistance patterns of farm isolates. the presence of genes linked to early mortality syndrome (ems) or acute hepatopancreatic necrosis disease (ahpnd) also was evaluated. vibrio spp. were isolated from retail shrimps in cuenca, ecuador, and farm shrimps originating from provinces el oro and guayas, ecuador. a total of 229 shrimp sampl ...	2015	26555534
prevalence of vibrio spp. in raw shrimps (parapenaeus longirostris) and performance of a chromogenic medium for the isolation of vibrio strains.	vibrios are natural inhabitants of estuarine ecosystems and some species may pose public health problem as agents of sporadic or collective food-borne infections associated with the consumption of fish or shellfish. samples of raw shrimp (n = 299), fished in coastal areas of the city of agadir, morocco, and collected from its fish marketplace, were examined for the presence of pathogenic vibrios. microbiological analysis was carried out according to a protocol using thiosulphate citrate bile suc ...	2015	26081523
development of triplex real-time pcr and detection of toxoplasma gondii dna in infected mice tissues and spiked human samples.	toxoplasma gondii is an important pathogen in veterinary and human medicine. in this study, a new multiplex taqman real-time pcr for detection of t. gondii dna was developed. this assay consisted of new sets of primers and probes which targeted b1 gene and its-1 region of t. gondii, with vibrio cholera gene as internal control. the b1 gene primers were designed to detect t. gondii rh strain, while the its-1 region primers detected most t. gondii strains. specificity test using common protozoal a ...	2015	26691266
genome-wide fitness and genetic interactions determined by tn-seq, a high-throughput massively parallel sequencing method for microorganisms.	the lagging annotation of bacterial genomes and the inherent genetic complexity of many phenotypes is hindering the discovery of new drug targets and the development of new antimicrobial agents and vaccines. this unit presents tn-seq, a method that has made it possible to quantitatively determine fitness for most genes in a microorganism and to screen for quantitative genetic interactions on a genome-wide scale and in a high-throughput fashion. tn-seq can thus direct studies on the annotation of ...	2015	25641100
prevalence of enteropathogenic bacteria in treated effluents and receiving water bodies and their potential health risks.	the failure of wastewater treatment plants to produce effluents of a high microbiological quality is a matter of great concern in terms of water resource pollution. a more serious concern is that this water source is used by communities in developing countries for multiple purposes, which include drinking, recreation and agriculture. the current study investigated the prevalence and potential health risks of enteropathogenic bacteria (salmonella typhimurium, shigella dysenteriae and vibrio chole ...	2015	25777950
phytochemical analysis and estimation of major bioactive compounds from triticum aestivum l. grass with antimicrobial potential.	the aim of the present study was to investigate phytochemical analysis, and qualitative and quantitative determination of major bioactive compound present in various organic extracts of t. aestivum l. grass. soxhlet apparatus was used for the extraction purpose using hexane, chloroform, methanol and distilled water as a solvent system. all the extracts derived from t. aestivum showed qualitative presence of major phytochemicals including alkaloids, steroids and cardiac glycosides tannins, flavon ...	2015	26687741
the relationship between glycan binding and direct membrane interactions in vibrio cholerae cytolysin, a channel-forming toxin.	bacterial pore-forming toxins (pfts) are structurally diverse pathogen-secreted proteins that form cell-damaging channels in the membranes of host cells. most pfts are released as water-soluble monomers that first oligomerize on the membrane before inserting a transmembrane channel. to modulate specificity and increase potency, many pfts recognize specific cell surface receptors that increase the local toxin concentration on cell membranes, thereby facilitating channel formation. vibrio cholerae ...	2015	26416894
halophilic and halotolerant actinomycetes from a marine saltern of goa, india producing anti-bacterial metabolites.	marine salterns are estuarine ecosystems in goa, receiving inputs from riverine and marine waters. the salinity fluctuates between 0 and 300 psu which makes it a conducive niche for salt tolerant and salt loving actinomycetales. halotolerant and halophilic actinomycetales producing anti-bacterial metabolites were studied from crystallizer pond sediments of ribandar saltern, goa. three media viz. starch casein, r2a and inorganic salt starch agar at four different salinities (35, 50, 75 and 100 ps ...	2015	25449757
using s. cerevisiae as a model system to investigate v. cholerae vopx-host cell protein interactions and phenotypes.	most pathogenic, non-o1/non-o139 serogroup vibrio cholerae strains cause diarrheal disease in the absence of cholera toxin. instead, many use type 3 secretion system (t3ss) mediated mechanisms to disrupt host cell homeostasis. we identified a t3ss effector protein, vopx, which is translocated into mammalian cells during in vitro co-culture. in a s. cerevisiae model system, we found that expression of vopx resulted in a severe growth defect that was partially suppressed by a deletion of rlm1, enc ...	2015	26473925
the two transmembrane helices of ccop are sufficient for assembly of the cbb3-type heme-copper oxygen reductase from vibrio cholerae.	the c-family (cbb3) of heme-copper oxygen reductases are proton-pumping enzymes terminating the aerobic respiratory chains of many bacteria, including a number of human pathogens. the most common form of these enzymes contains one copy each of 4 subunits encoded by the cconoqp operon. in the cbb3 from rhodobacter capsulatus, the enzyme is assembled in a stepwise manner, with an essential role played by an assembly protein ccoh. importantly, it has been proposed that a transient interaction betwe ...	2015	26116881
the role of flhf and hubp as polar landmark proteins in shewanella putrefaciens cn-32.	spatiotemporal regulation of cell polarity plays a role in many fundamental processes in bacteria and often relies on 'landmark' proteins which recruit the corresponding clients to their designated position. here, we explored the localization of two multi-protein complexes, the polar flagellar motor and the chemotaxis array, in shewanella putrefaciens cn-32. we demonstrate that polar positioning of the flagellar system, but not of the chemotaxis system, depends on the gtpase flhf. in contrast, t ...	2015	26235439
transcriptome analysis revealed changes of multiple genes involved in immunity in cynoglossus semilaevis during vibrio anguillarum infection.	half-smooth tongue sole (cynoglossus semilaevis) is one of the most valuable marine aquatic species in northern china. given to the rapid development of aquaculture industry, the c. semilaevis was subjected to disease-causing bacteria vibrio anguillarum. it therefore is indispensable and urgent to understand the mechanism of c. semilaevis host defense against v. anguillarum infection. in the present study, the extensively analysis at the transcriptome level for v. anguillarum disease in tongue s ...	2015	25543033
isolation of vibrio cholerae and other enteric microbiota from patients with suspected cholera during the 2009 outbreak in madang province, papua new guinea.	when cholera was first detected in papua new guinea (png) in mid-2009, national diagnostic capacity faced many challenges. this was in part due to the non-endemic status of the outbreak, resulting in few local staff experienced in vibrio cholerae detection and poor access to the required consumables. the png institute of medical research conducted culture on specimens from suspected cholera patients in madang province, with presumptive v. cholerae isolates sent to goroka for confirmation. of 98 ...	2015	26288927
discovery of a bifunctional acyltransferase responsible for ornithine lipid synthesis in serratia proteamaculans.	ornithine lipids (ols) are phosphorus-free membrane lipids that can be formed by many bacteria but that are absent from archaea and eukaryotes. a function for ols in stress conditions and in host-bacteria interactions has been shown in some bacteria. some bacterial species have been described that can form ols, but lack the known genes (olsba) involved in its biosynthesis, which implied the existence of a second pathway. here we describe the bifunctional protein olsf from serratia proteamaculans ...	2015	25040623