Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| pathogen-induced tlr4-trif innate immune signaling in hematopoietic stem cells promotes proliferation but reduces competitive fitness. | bacterial infection leads to consumption of short-lived innate immune effector cells, which then need to be replenished from hematopoietic stem and progenitor cells (hspcs). hspcs express pattern recognition receptors, such as toll-like receptors (tlrs), and ligation of these receptors induces hspc mobilization, cytokine production, and myeloid differentiation. the underlying mechanisms involved in pathogen signal transduction in hscs and the resulting biological consequences remain poorly defin ... | 2017 | 28736216 |
| eucalyptol, sabinene and cinnamaldehyde: potent inhibitors of salmonella target protein l-asparaginase. | salmonella typhimurium is a severe threat to human life. the treatment became more difficult with the emergence of multidrug resistance. in the present in silico study, a novel drug target l-asparaginase was tested against three ligands eucalyptol, sabinene, and cinnamaldehyde, major components of cardamom, nutmeg, and cinnamon, respectively. the lowest docking score was obtained for sabinene followed by eucalyptol and cinnamaldehyde i.e. -5.648, -3.939 and -3.469. the docking score of sabinene ... | 2017 | 28735432 |
| current perspectives on invasive nontyphoidal salmonella disease. | we searched pubmed for scientific literature published in the past 2 years for relevant information regarding the burden of invasive nontyphoidal salmonella disease and host factors associated with nontyphoidal salmonella infection and discuss current knowledge on vaccine development. the following search terms were used: salmonella, non typhoidal/nontyphoidal, nts, disease, bloodstream infection, invasive, sepsis/septicaemia/septicemia, bacteraemia/bacteremia, gastroenteritis, incidence, preval ... | 2017 | 28731899 |
| pen-on-paper strategy for point-of-care testing: rapid prototyping of fully written microfluidic biosensor. | paper-based microfluidic biosensors have recently attracted increasing attentions in point-of-care testing (poct) territories benefiting from their affordable, accessible and eco-friendly features, where technologies for fabricating such biosensors are preferred to be equipment free, easy-to-operate and capable of rapid prototyping. in this work, we developed a pen-on-paper (pop) strategy based on two custom-made pens, i.e., a wax pen and a conductive-ink pen, to fully write paper-based microflu ... | 2017 | 28728008 |
| development of a salmonella typhimurium challenge model in weaned pigs to evaluate effects of water and feed interventions on fecal shedding and growth performance. | the aim of this study was to develop a typhimurium (st) challenge model in weaned pigs suitable to evaluate effects of water and feed interventions on fecal shedding and growth performance. two studies were performed. in exp. 1 weaned pigs were fed either a standard diet (con) or a diet with a high buffer capacity (hb) and challenged for either 3 or 7 consecutive days in a latin square design with 4 × 8 individually housed pigs. in exp. 2, the con 7-d challenge method was chosen for further mode ... | 2017 | 28727108 |
| a novel pathogen capturing device for removal and detection. | a simple technique that employs an antibody coated polydimethylsiloxane tube is used for effective capturing of bloodborne and foodborne pathogens. by recirculating the entire sample through the antibody coated tube, accumulation of target pathogens is achieved, thereby delivering a higher concentration of pathogens in a small volume. the described method can provide an effective and economical solution to microbiology techniques that rely on enrichment, thereby expediting diagnostics. using thi ... | 2017 | 28717239 |
| th17-mediated cross protection against pneumococcal carriage by vaccination with a variable antigen. | serotype-specific protection against a streptococcus pneumoniae is an important limitation of the current polysaccharide-based vaccines. to prevent serotype replacement, reduce transmission and limit emergence of new variants, it is essential to induce broad protection and restrict pneumococcal colonization. in this study, we used a prototype vaccine formulation consisting of lps-detoxified outer membrane vesicles (omvs) from salmonella typhimurium displaying the variable n-terminus of pspa (α1α ... | 2017 | 28717032 |
| molecular dynamics studies on the domain swapped salmonella typhimurium survival protein sure: insights on the possible reasons for catalytic cooperativity. | stationary phase survival protein sure from salmonella typhimurium is a dimeric protein formed by the swapping of a tetramerization loop involved in the formation of a loose tetramer and a c-terminal helix. it functions as a phosphatase. the two-fold symmetry of the dimeric protein was lost in the mutants h234a and d230a/h234a in which a crucial hydrogen bond in the hinge involved in c-terminal helix swapping was eliminated. the catalytic activity of both mutants was drastically reduced. in cont ... | 2017 | 28714824 |
| growth characterization of single and double salmonella methionine auxotroph strains for potential vaccine use in poultry. | poultry meat is an important source of zoonotic salmonella infection. oral vaccination of chickens with live attenuated salmonella during grow-out is an attractive approach to control salmonella colonization in the chicken gastrointestinal tract. in this study, we report the construction of methionine-dependent and growth of salmonella typhimurium mutant strains with methionine auxotrophy (δmetr and δδmetrmetd) and survival in chicken feed and fecal matrices. the methionine auxotroph mutant δδme ... | 2017 | 28706896 |
| antimutagenic and antioxidant activities of thai rice brans. | rice bran is the outer layer of the rice grain, and contains high amounts of bioactive phytochemicals. here, we investigated and compared chemopreventive properties of purple and white rice bran extracts. | 2017 | 28698862 |
| investigation of medium chain fatty acid feed supplementation for reducing salmonella typhimurium colonization in turkey poults. | studies indicate that persistent salmonella colonization occurs in poultry that are infected early in life, leading to both food safety and public health concerns. development of improved preharvest salmonella management strategies is needed to reduce poultry product contamination. the objective of this study was to evaluate the efficacy of a product containing medium chain fatty acids (mcfa) for reducing early salmonella colonization in turkey poults. day-of-hatch turkeys were provided a standa ... | 2017 | 28696788 |
| seeing but not believing: the structure of glycerol dehydrogenase initially assumed to be the structure of a survival protein from salmonella typhimurium. | the determination of the crystal structure of a mutant protein using phases based on a previously determined crystal structure of the wild-type protein is often a straightforward molecular-replacement protocol. such a structure determination may be difficult if there are large-scale structural differences between the wild-type and mutant proteins. in this manuscript, an interesting case is presented of the unintentional crystallization of a contaminant protein which shared some structural featur ... | 2017 | 28695861 |
| genotoxic and cytotoxic potential of whole plant extracts of kalanchoe laciniata by ames and mtt assay. | lack of data on safety of herbal medicines have endangered human health and life. the present study evaluated the genotoxic and mutagenic effect of kalanchoe laciniata to access the safety and usefulness of the medicinal plant. aqua-methanolic and n-hexane extracts of k. laciniata were evaluated for the genotoxic potential using ames assay and cytotoxicity was evaluated using mtt assay. ames assay was conducted using two strains of salmonella typhimurium ta-100 and ta-102 whereas mtt assay was p ... | 2017 | 28694760 |
| culling of apcs by inflammatory cell death pathways restricts tim3 and pd-1 expression and promotes the survival of primed cd8 t cells. | we evaluated the impact of premature cell death of antigen-presenting cells (apcs) by caspase-1- and ripk3-signaling pathways on cd8(+) t-cell priming during infection of mice with salmonella typhimurium (st). our results indicate that caspase1 and ripk3 synergize to rapidly eliminate infected apcs, which does not influence the initial activation of cd8(+) t cells. however, the maintenance of primed cd8(+) t cells was greatly compromised when both these pathways were disabled. caspase-1- and rip ... | 2017 | 28686578 |
| mesenchymal cell-specific myd88 signaling promotes systemic dissemination of salmonella typhimurium via inflammatory monocytes. | enteric pathogens including salmonella enteric serovar typhimurium can breach the epithelial barrier of the host and spread to systemic tissues. in response to infection, the host activates innate immune receptors via the signaling molecule myd88, which induces protective inflammatory and antimicrobial responses. most of these innate immune responses have been studied in hematopoietic cells, but the role of myd88 signaling in other cell types remains poorly understood. surprisingly, we found tha ... | 2017 | 28674182 |
| temporal regulation of a salmonella typhimurium virulence factor by the transcriptional regulator ydcr. | we previously examined salmonella proteome within infected host cells and found differential expression of many proteins with defined functional roles such as metabolism or virulence. however, the precise roles of other altered proteins in salmonella pathogenesis are largely unknown. a putative transcriptional regulator, ydcr, was highly induced intracellularly while barely expressed in vitro, implicating potential relevance to bacterial infection. to unveil its physiological functions, we explo ... | 2017 | 28674150 |
| original xpc(tm) effect on salmonella typhimurium and cecal microbiota from three different ages of broiler chickens when incubated in an anaerobic in vitro culture system. | feed supplements are utilized in the poultry industry as a means for improving growth performance and reducing pathogens. the aim of the present study was to evaluate the effects of diamond v original xpc(tm) (xpc, a fermented product generated from yeast cultures) on salmonella typhimurium st 97 along with its potential for modulation of the cecal microbiota by using an anaerobic in vitro mixed culture assay. cecal slurries obtained from three broiler chickens at each of three sampling ages (14 ... | 2017 | 28659891 |
| an aptamer-based pcr method coupled with magnetic immunoseparation for sensitive detection of salmonella typhimurium in ground turkey. | aptamers are single-stranded oligonucleotide ligands that can bind to targets with high affinity and specificity. they have been widely studied in the field of diagnostics as alternatives to antibodies due to their favorable features such as easy labeling, temperature tolerance, lower cost and recognition of a wide variety of targets. in this study, an aptamer-based pcr method coupled with magnetic immunoseparation was developed to detect s. typhimurium from ground turkey. firstly, biotinylated ... | 2017 | 28645756 |
| dietary salt exacerbates experimental colitis. | the western diet is characterized by high protein, sugar, fat, and low fiber intake, and is widely believed to contribute to the incidence and pathogenesis of inflammatory bowel disease (ibd). however, high sodium chloride salt content, a defining feature of processed foods, has not been considered as a possible environmental factor that might drive ibd. we set out to bridge this gap. we examined murine models of colitis on either a high salt diet (hsd) or a low salt diet. we demonstrate that an ... | 2017 | 28637899 |
| effects of the surface modification of polyurethane substrates on genotoxicity and blood activation processes. | the aim of this study was to determine the mutagenic and thrombogenic potential of a material composed of a thin coating deposited on a polymeric substrate. in this work, a surface was modified in a manner that would mimic the function of cellular niches. finally, the surfaces should actively capture and differentiate progenitor cells from the blood stream. thin films with 10 to 500nm thicknesses were deposited by unbalanced, pulsed dc magnetron sputtering on smooth polyurethane. such high energ ... | 2017 | 28629077 |
| tumor-targeting salmonella typhimurium a1-r promotes tumoricidal cd8(+) t cell tumor infiltration and arrests growth and metastasis in a syngeneic pancreatic-cancer orthotopic mouse model. | the present study determined the effect of the tumor-targeting strain salmonella typhimurium a1-r (s. typhimurium a1-r) on cd8(+) tumor-infiltrating lymphocytes (tils) in a syngeneic pancreatic-cancer orthotopic mouse model. the effect of tumor-targeting s. typhimurium a1-r on cd8(+) tils was determined on the pan02 murine pancreatic-adenocarcinoma implanted orthotopically in the pancreatic tail of c57bl/6 immunocompromised mice. three weeks after orthotopic implantation, mice were randomized as ... | 2017 | 28628234 |
| in vitro biotransformation, safety, and chemopreventive action of novel 8-methoxy-purine-2,6-dione derivatives. | metabolic stability, mutagenicity, antimutagenicity, and the ability to scavenge free radicals of four novel 8-methoxy-purine-2,6-dione derivatives (compounds 1-4) demonstrating analgesic and anti-inflammatory properties were determined. metabolic stability was evaluated in cunninghamella and microsomal models, mutagenic and antimutagenic properties were assessed using the ames and the vibrio harveyi tests, and free radical scavenging activity was evaluated with 2,2-diphenyl-1-picrylhydrazyl rad ... | 2017 | 28624999 |
| nonclinical safety of astilbin: a 4-week oral toxicity study in rats with genotoxicity, chromosomal aberration, and mammalian micronucleus tests. | astilbin is an active flavonoid compound isolated from rhizoma smilacis glabrae. it has been widely used as an anti-hepatic, anti-arthritic, and anti-renal injury agent. however, its safety has not yet been established. the objective of this study was to evaluate 4-week repeated oral toxicity and genotoxicity of astilbin. we examined oral toxicity in sprague-dawley rats after daily oral administration of astilbin at 50, 150, and 500 mg/kg for 4 weeks. negative control animals received the same v ... | 2017 | 28624472 |
| aptasensors for quantitative detection of salmonella typhimurium. | salmonella is one of the most frequent causes of food borne infectious disease. among nearly 2500 documented serotypes are reported, salmonella typhimurium is the number one serotype associated with salmonellosis worldwide. many different methods have been developed for the detection and quantification of s. typhimurium. most of these assays are usually expensive, time consuming and require difficult sample preparation steps. therefore, it is necessary to develop rapid, robust, cost-effective an ... | 2017 | 28624297 |
| the index of ideality of correlation: a criterion of predictive potential of qspr/qsar models? | the index of ideality of correlation (iic) is a new criterion of the predictive potential of quantitative structure-property/activity relationships (qsprs/qsars). this iic is calculated with using of the correlation coefficient between experimental and calculated values of endpoint for the calibration set, with taking into account the positive and negative dispersions between experimental and calculated values. the mutagenicity is well-known important characteristic of substances from ecological ... | 2017 | 28622828 |
| salmonella typhimurium a1-r targeting of a chemotherapy-resistant braf-v600e melanoma in a patient-derived orthotopic xenograft (pdox) model is enhanced in combination with either vemurafenib or temozolomide. | a metastatic melanoma obtained from the right chest wall of a patient was previously established orthotopically in the right chest wall of nude mice as a patient-derived orthotopic xenograft (pdox) model. we previously showed that the combination of tumor-targeting salmonella typhimurium a1-r (s. typhimurium a1-r) and chemotherapy was highly effective against the melanoma pdox. in the present study, we investigated the mechanism of the high efficacy of this combination. two weeks after implantat ... | 2017 | 28622068 |
| enhancement of ifnγ production by distinct commensals ameliorates salmonella-induced disease. | the microbiota contributes to colonization resistance against invading pathogens by competing for metabolites, producing inhibitory substances, and priming protective immune responses. however, the specific commensal bacteria that promote host resistance and immune-mediated protection remain largely elusive. using isogenic mouse lines with distinct microbiota profiles, we demonstrate that severity of disease induced by enteric salmonella typhimurium infection is strongly modulated by microbiota ... | 2017 | 28618267 |
| gut microbiota-mediated protection against diarrheal infections. | the mammalian gut microbiota is a highly abundant and diverse microbial community that resides in the gastrointestinal tract. one major benefit that the gut microbiota provides to its host is colonization resistance-the ability to prevent colonization by foreign microbes, including diarrheal pathogens such as clostridium difficile , salmonella enterica serovar typhimurium and diarrheagenic escherichia coli . | 2017 | 28520994 |
| increase in nitrite content and functionality of ethanolic extracts of perilla frutescens following treatment with atmospheric pressure plasma. | this study investigated the effect of atmospheric pressure plasma (app) treatment on nitrite content and functionality of plant extracts. ethanolic extracts of perilla frutescens (eep) were prepared and treated with app for 60min. nitrite content increased from 0 to 45.8mg/l in eep after app treatment for 60min. antimicrobial activity of eep against clostridium perfringens and salmonella typhimurium was increased by app with no influence on antioxidative activity (p<0.05). lyophilized eep (leep) ... | 2017 | 28763985 |
| modeling the heat inactivation of foodborne pathogens in milk powder: high relevance of the substrate water activity. | due to the ability of foodborne pathogens to survive in low moisture foods, the decontamination of these products is an important issue in food hygiene. up to now, such decontamination has mostly been achieved through empirical methods. the intention of this work is to establish a more rational use of heat treatment cycles. the effects of thermal treatment cycles on the inactivation of dried salmonella typhimurium, salmonella senftenberg, cronobacter sakazakii and escherichia coli were assessed. ... | 2017 | 28784519 |
| exploitation of spr to investigate the importance of glycan chains in the interaction between lactoferrin and bacteria. | bovine lactoferrin (lf) has been shown to prevent adhesion to and invasion of mammalian cell lines by pathogenic bacteria, with evidence for direct bacterial binding by the milk glycoprotein. however, the glycosylation pattern of lf changes over the lactation cycle. in this study, we aim to investigate the effect that this variation has on the milk glycoprotein's ability to interact with pathogens. surface plasmon resonance technology was employed to compare the binding of lf from colostrum (ear ... | 2017 | 28653977 |
| participation of 14-3-3ε and 14-3-3ζ proteins in the phagocytosis, component of cellular immune response, in aedes mosquito cell lines. | better knowledge of the innate immune system of insects will improve our understanding of mosquitoes as potential vectors of diverse pathogens. the ubiquitously expressed 14-3-3 protein family is evolutionarily conserved from yeast to mammals, and at least two isoforms of 14-3-3, the ε and ζ, have been identified in insects. these proteins have been shown to participate in both humoral and cellular immune responses in drosophila. as mosquitoes of the genus aedes are the primary vectors for arbov ... | 2017 | 28764795 |
| a new microfluidic approach for the one-step capture, amplification and label-free quantification of bacteria from raw samples. | a microfluidic method to specifically capture and detect infectious bacteria based on immunorecognition and proliferative power is presented. it involves a microscale fluidized bed in which magnetic and drag forces are balanced to retain antibody-functionalized superparamagnetic beads in a chamber during sample perfusion. captured cells are then cultivated in situ by infusing nutritionally-rich medium. the system was validated by the direct one-step detection of salmonella typhimurium in undilut ... | 2017 | 28626552 |
| a new epipolythiodioxopiperazine with antibacterial and cytotoxic activities from the endophytic fungus chaetomium sp. m336. | a new compound 6-formamide-chetomin (1) together with chetomin (2) was isolated from solid fermentation products of the endophytic fungus chaetomium sp. m336, which were elucidated by extensive spectroscopic analyses, including 1d and 2d nmr, and hr-esi-ms experiments. the bioassay result showed that compound 1 had strong antibacterial activity against escherichia coli, staphylococcus aureus, salmonella typhimurium atcc 6539 and enterococcus faecalis with minimum inhibitory concentration (mic) v ... | 2017 | 28602097 |
| pathobactyper: a web server for pathogenic bacteria identification and molecular genotyping. | with the decline in the cost of whole-genome sequencing because of the introduction of next-generation sequencing (ngs) techniques, many public health and clinical laboratories have started to use bacterial whole genomes for epidemiological surveillance and clinical investigation. for epidemiological and clinical purposes in this "ngs era," whole-genome-scale single nucleotide polymorphism (wgsnp) analysis for genotyping is considered suitable. in this paper, we present an online service, pathob ... | 2017 | 28824598 |
| ultraviolet (uv-c) inactivation of enterococcus faecium, salmonella choleraesuis and salmonella typhimurium in porcine plasma. | the objective of this study was to assess the effectiveness of an ultraviolet (uv-c, 254 nm) irradiation system on reducing the load of salmonella typhimurium (s. typhimurium), salmonella choleraesuis (s. choleraesuis) resistant to streptomycin and enterococcus faecium (e. faecium) inoculated in sterile porcine plasma and then subjected to different uv-c irradiation doses (750, 1500, 3000, 6000 and 9000 j/l) using a pilot plant uv-c device working under turbulent flow. results indicated that uv- ... | 2017 | 28399166 |
| comparison of the microbiological quality and safety between conventional and organic vegetables sold in malaysia. | given the remarkable increase of public interest in organic food products, it is indeed critical to evaluate the microbiological risk associated with consumption of fresh organic produce. organic farming practices including the use of animal manures may increase the risk of microbiological contamination as manure can act as a vehicle for transmission of foodborne pathogens. this study aimed to determine and compare the microbiological status between organic and conventional fresh produce at the ... | 2017 | 28824567 |
| short communication: detection of stx2 and elt genes in bovine milk by using a multiplex pcr system. | the aim of this study was to detect 2 important toxin genes from diarrheagenic escherichia coli (dec) in bovine milk using a new multiplex pcr. to standardize the multiplex pcr, the stx2 and elt genes were investigated for the detection of shiga toxin-producing escherichia coli (stec) and enterotoxigenic e. coli (etec), respectively. the dna template was prepared with a thermal procedure (boiling) and a commercial kit. samples consisted of uht and pasteurized milk, both skimmed, and stec and ete ... | 2017 | 28803013 |
| vitamin b12 as a carrier of peptide nucleic acid (pna) into bacterial cells. | short modified oligonucleotides targeted at bacterial dna or rna could serve as antibacterial agents provided that they are efficiently taken up by bacterial cells. however, the uptake of such oligonucleotides is hindered by the bacterial cell wall. to overcome this problem, oligomers have been attached to cell-penetrating peptides, but the efficiency of delivery remains poor. thus, we have investigated the ability of vitamin b12 to transport peptide nucleic acid (pna) oligomers into cells of es ... | 2017 | 28794451 |
| rpon2- and flia-regulated flitx is indispensible for flagellar motility and virulence in xanthomonas oryzae pv. oryzae. | bacterial blight of rice caused by xanthomonas oryzae pv. oryzae (xoo) is one of the most important crop diseases in the world. more insights into the mechanistic regulation of bacterial pathogenesis will help us identify novel molecular targets for developing effective disease control strategies. a large flagellar gene cluster is regulated under a three-tiered hierarchy by σ(54) factor rpon2 and its activator fleq, and σ(28) factor flia. a hypothetical protein gene flitx is located upstream of ... | 2017 | 28793882 |
| synthetic porcine hepcidin exerts opposite roles in escherichia coli and salmonella infections. | hepcidin, an antimicrobial peptide, was discovered to integrate diverse signals from iron status and infection threat and orchestrate a series of host protective responses. there have been several studies investigating the antimicrobial role of hepcidin but controversial. here, we aim to examine the role of hepcidin in bacterial adherence and invasion in vitro we found that porcine hepcidin could decrease the amount of extracellular pathogen enterotoxigenic escherichia coli (etec) k88 that adher ... | 2017 | 28760901 |
| antibacterial electrospun chitosan-based nanofibers: a bacterial membrane perforator. | this study investigates the antibacterial action of chitosan-based nanofibers (cnfs) obtained by the electrospinning process on the permeability of bacterial membranes. the bactericidal efficiency of cnfs was first determined against gram-negative escherichia coli and salmonella typhimurium, and gram-positive staphylococcus aureus and listeria innocua bacteria as a baseline. the results strongly suggest that cnfs interact with the negatively charged bacterial cell wall causing membrane rupture a ... | 2017 | 28748074 |
| anti-adhesion and anti-biofilm potential of organosilane nanoparticles against foodborne pathogens. | nowadays, modification of surfaces by nanoparticulate coatings is a simple process that may have applications in reducing the prevalence of bacterial cells both on medical devices and food processing surfaces. to this direction, biofilm biological cycle of salmonella typhimurium, listeria monocytogenes, escherichia coli o157:h7, staphylococcus aureus, and yersinia enterocolitica on stainless steel and glass surfaces, with or without nanocoating was monitored. to achieve this, four different comm ... | 2017 | 28744277 |
| vasoactive intestinal polypeptide and mast cells regulate increased passage of colonic bacteria in patients with irritable bowel syndrome. | irritable bowel syndrome (ibs) is associated with intestinal dysbiosis and symptoms of ibs develop following gastroenteritis. we aimed to study passage of live bacteria through the colonic epithelium, and determine the role of mast cells and vasoactive intestinal polypeptide (vip) in barrier regulation in ibs and healthy individuals. | 2017 | 28711627 |
| modeling polymorphic transformation of rotating bacterial flagella in a viscous fluid. | the helical flagella that are attached to the cell body of bacteria such as escherichia coli and salmonella typhimurium allow the cell to swim in a fluid environment. these flagella are capable of polymorphic transformation in that they take on various helical shapes that differ in helical pitch, radius, and chirality. we present a mathematical model of a single flagellum described by kirchhoff rod theory that is immersed in a fluid governed by stokes equations. we perform numerical simulations ... | 2017 | 28709256 |
| structural studies on the decameric s. typhimurium arginine decarboxylase (adc): pyridoxal 5'-phosphate binding induces conformational changes. | enteric pathogens such as salmonella typhimurium colonize the human gut in spite of the lethal acidic ph environment (ph < 2.5) due to the activation of inducible acid tolerance response (atr) systems. the pyridoxal 5'-phosphate (plp)-dependent enzyme, biodegradative arginine decarboxylase (adc, encoded by adia), is a component of an atr system. the enzyme consumes a cytoplasmic proton in the process of arginine degradation to agmatine. arginine-agmatine antiporter (adic) exchanges the product a ... | 2017 | 28694189 |
| role of the inner-membrane histidine kinase rcsc and outer-membrane lipoprotein rcsf in the activation of the rcs phosphorelay signal transduction system in escherichia coli. | the rcs phosphorelay signal transduction system of escherichia coli controls genes for capsule production and many other envelope-related functions and is implicated in biofilm formation. the outer-membrane lipoprotein rcsf is an essential component of the rcs system. mislocalization of rcsf to the periplasm or the cytoplasmic membrane leads to high activation of the rcs system, suggesting that rcsf functions by interacting with the cytoplasmic membrane component(s) of the system in activating t ... | 2017 | 28691662 |
| genetic damage induced by electronic waste leachates and contaminated underground water in two prokaryotic systems. | the inappropriate and unsafe management practices related to disposal and recycling of electronic wastes in nigeria has led to environmental and underground water contamination. reports on the level and type of contamination as well as the possible dna damage effects of this contamination are insufficient. this study evaluated the dna damaging potential of e-waste simulated and raw leachates, and its contaminated underground water using the sos chromotest on escherichia coli pq37 and the ames sa ... | 2017 | 28670935 |
| blue native page analysis of bacterial secretion complexes. | bacterial protein secretion systems serve to translocate substrate proteins across up to three biological membranes, a task accomplished by hydrophobic, membrane-spanning macromolecular complexes. the overexpression, purification, and biochemical characterization of these complexes is often difficult, impeding progress in understanding the structure and function of these systems. blue native (bn) polyacrylamide gel electrophoresis (page) allows for the investigation of these transmembrane comple ... | 2017 | 28667624 |
| effects of heat treatment on the antioxidative and anti-inflammatory properties of orange by-products. | this study investigated the changes in the functional components, antioxidative activities, antibacterial activities, anti-inflammatory activities of orange (citrus sinensis (l.) osbeck) by-products (obp) on heat treatment at 50 and 100 °c (hereafter denoted 50d and 100d extracts, respectively). optimal heating conditions were also investigated. the total phenolic content, flavonoid content and antioxidative activities of obp extracts significantly increased on heat treatment. the lag time of cu ... | 2017 | 28653733 |
| host and pathogen copper-transporting p-type atpases function antagonistically during salmonella infection. | copper is an essential yet potentially toxic trace element that is required by all aerobic organisms. a key regulator of copper homeostasis in mammalian cells is the copper-transporting p-type atpase atp7a, which mediates copper transport from the cytoplasm into the secretory pathway, as well as copper export across the plasma membrane. previous studies have shown that atp7a-dependent copper transport is required for killing phagocytosed escherichia coli in a cultured macrophage cell line. in th ... | 2017 | 28652309 |
| epigallocatechin gallate inhibits the type iii secretion system of gram-negative enteropathogenic bacteria under model conditions. | epigallocatechin gallate (egcg), a major polyphenol in green tea, inhibits the type iii secretion system (t3ss) of enteropathogenic and enterohemorrhagic escherichia coli (epec and ehec, respectively), salmonella enterica serovar typhimurium, and yersinia pseudotuberculosis. the inhibitory effect causes the inhibition of hemolysis, cell invasion, cell adhesion and apoptosis, which are functions of the type iii secretion device. in the case of epec, espb accumulates in the cells. rt-pcr showed th ... | 2017 | 28651361 |
| local application of bacteria improves safety of salmonella -mediated tumor therapy and retains advantages of systemic infection. | cancer is a devastating disease and a large socio-economic burden. novel therapeutic solutions are on the rise, although a cure remains elusive. application of microorganisms represents an ancient therapeutic strategy, lately revoked and refined via simultaneous attenuation and amelioration of pathogenic properties. salmonella typhimurium has prevailed in preclinical development. yet, using virulent strains for systemic treatment might cause severe side effects. in the present study, we highligh ... | 2017 | 28637010 |
| physicochemical characteristics, mutagenicity and genotoxicity of airborne particles under industrial and rural influences in northern lebanon. | in this work, the main objectives were to assess the mutagenic and genotoxic effects of fine particulate matter collected in an industrial influenced site in comparison with a non-industrial influenced one (rural site) and to relate the particulate matter (pm) composition to the observed genotoxic effects. at the industrial influenced site, higher concentrations of phosphates, trace metals, and polycyclic aromatic hydrocarbons (pahs) in particles could be related to the contributions of quarries ... | 2017 | 28620855 |
| bdm-mediated regulation of flagellar biogenesis in escherichia coli and salmonella enterica serovar typhimurium. | synthesis of the flagellar apparatus in escherichia coli is mediated via complex regulatory pathways. a previous study indicated that the protein encoded by the biofilm-dependent modulation (bdm) gene is linked closely with a regulatory pathway for flagellar assembly. however, the specific role of bdm in flagellar biogenesis remains unknown. herein, we showed that bdm interacts with flgm and inhibits its function as an anti-σ28 factor, which induces the transcription of flagellar late-class gene ... | 2017 | 28603807 |
| ames test to detect mutagenicity of 2-alkylcyclobutanones: a review. | food irradiation is an effective and safe method for preservation and long-term storage, and it is approved for use in over 60 countries for various applications in a wide variety of food products. this process is performed by use of accelerated electron beams, x-rays, or gamma radiation ((60) co or (137) cs). 2-alkylcyclobutanones (2-acbs) are the only known radiolytic products generated from foods that have fatty acids (triglycerides) and are subjected to irradiation. since the 1990s toxicolog ... | 2017 | 28598571 |
| the rcs-regulated colanic acid capsule maintains membrane potential in salmonella enterica serovar typhimurium. | the rcs phosphorelay and psp (phage shock protein) systems are envelope stress responses that are highly conserved in gammaproteobacteria. the rcs regulon was found to be strongly induced during metal deprivation of salmonella enterica serovar typhimurium lacking the psp response. nineteen genes activated by the rcsa-rcsb response regulator make up an operon responsible for the production of colanic acid capsular polysaccharide, which promotes biofilm development. despite more than half a centur ... | 2017 | 28588134 |
| impact of sweet potato starch-based nanocomposite films activated with thyme essential oil on the shelf-life of baby spinach leaves. | salmonella typhimurium (s. typhi) and escherichia coli (e. coli) have been responsible for an increasing number of outbreaks linked to fresh produce, such as baby spinach leaves, in the last two decades. more recently, antimicrobial biodegradable packaging systems have been attracting much attention in the food packaging industry as eco-friendly alternatives to conventional plastic packaging. the objective of this study was to evaluate the effect of antibacterial nanocomposite films on inoculate ... | 2017 | 28587199 |
| evaluation of genetic toxicity of 6-diazo-5-oxo-l-norleucine (don). | don (6-diazo-5-oxo-l-norleucine), a glutamine antagonist, was demonstrated to exhibit analgesic, antibacterial, antiviral and anticancer properties. the study was performed to characterize its in vitro and in vivo genetic toxicity potential. don was tested in the bacterial reverse mutation assay (ames test) using salmonella typhimurium tester strains (ta98, ta100, ta1535 and ta1537) and escherichia coli tester strain (wp2 uvra) with and without s9 and also with reductive s9. in addition, don was ... | 2017 | 28552037 |
| antibiofilm activity and mode of action of dmso alone and its combination with afatinib against gram-negative pathogens. | biofilms are complex microbial communities that tend to attach to either biotic or abiotic surface. enclosed in a self-produced extracellular polymeric substance (eps) matrix, the biofilms often cause persistent infections. the objective of this study was to investigate the antibiofilm activity of dimethyl sulfoxide (dmso) and afatinib against gram-negative pathogens. test microorganisms used in this study were escherichia coli atcc 1299, pseudomonas aeruginosa atcc 10145, and salmonella typhimu ... | 2017 | 28540585 |
| detecting and discriminating shigella sonnei using an aptamer-based fluorescent biosensor platform. | in this paper, a whole-bacteria selex (wb-selex) strategy was adopted to isolate specific aptamers against shigella sonnei. real-time pcr amplification and post-selex experiment revealed that the selected aptmers possessed a high binding affinity and specificity for s. sonnei. of the 21 aptamers tested, the c(t) values of the ss-3 and ss-4 aptamers (ct = 13.89 and ct = 12.23, respectively) had the lowest value compared to other aptamer candidates. the ss-3 and ss-4 aptamers also displayed a bind ... | 2017 | 28513559 |
| large-scale recoding of a bacterial genome by iterative recombineering of synthetic dna. | the ability to rewrite large stretches of genomic dna enables the creation of new organisms with customized functions. however, few methods currently exist for accumulating such widespread genomic changes in a single organism. in this study, we demonstrate a rapid approach for rewriting bacterial genomes with modified synthetic dna. we recode 200 kb of the salmonella typhimurium lt2 genome through a process we term sircas (stepwise integration of rolling circle amplified segments), towards const ... | 2017 | 28499033 |
| anco3, a new member of the emerging family of phage-like plasmids. | a phage-like plasmid isolated from a clinical isolate of salmonella enterica serovar derby has strong nucleotide sequence identity to the phage-like plasmids pstm_phi isolated from salmonella enterica serovar typhimurium l495, anco1 and anco2 from escherichia coli 243 and escherichia coli 244, and the virulent salmonella-specific ssu5 bacteriophage. | 2017 | 28495760 |
| assessment of consumer exposure to salmonella spp., campylobacter spp., and shiga toxin-producing escherichia coli in meat products at retail in the city of sao paulo, brazil. | meat products may be vehicles of bacterial pathogens to humans, and salmonella spp., campylobacter spp., and shiga toxin-producing escherichia coli (stec) are the most relevant. the aim of this study was to generate data on prevalence of these three pathogens in 552 samples of meat products (hot dogs, pork sausages, raw ground beef, and raw chicken legs) sold at retail in the city of sao paulo, brazil. salmonella spp. was detected in 5.8% (32/552) of samples, comprising pork sausages 62.5% (20/3 ... | 2017 | 28475359 |
| the legacy of genetic analysis advances contemporary research with escherichia coli k-12 and salmonella enterica serovar typhimurium lt2. | escherichia coli k-12 and salmonella enterica serovar typhimurium lt2 became standard organisms for genetic analysis during the truman administration. half a century later, genetic analysis with these strains had become an art form, interpreted through 23 articles in the ambitious two-volume masterpiece edited by the late fred neidhardt and colleagues. these legacy articles now are available through ecosal plus, so as to inform and inspire contemporary genetic analyses in these standard organism ... | 2017 | 28443537 |
| virulence of invasive salmonella typhimurium st313 in animal models of infection. | salmonella typhimurium sequence type (st) 313 produces septicemia in infants in sub-saharan africa. although there are known genetic and phenotypic differences between st313 strains and gastroenteritis-associated st19 strains, conflicting data about the in vivo virulence of st313 strains have been reported. to resolve these differences, we tested clinical salmonella typhimurium st313 and st19 strains in murine and rhesus macaque infection models. the 50% lethal dose (ld50) was determined for thr ... | 2017 | 28783750 |
| characterization of antimicrobial properties of salmonella phage felix o1 and listeria phage a511 embedded in xanthan coatings on poly(lactic acid) films. | beyond simply providing a barrier between food and external contaminants, active packaging technologies aim to inhibit pathogen survival and growth within the packaged environment. bacteriophages have a proven track record as targeted antimicrobials but have yet to be successfully integrated in active packaging without serious loss of activity. we have developed two bacteriophage based xanthan coatings on poly(lactic acid) (pla) film which significantly inhibits salmonella typhimurium and lister ... | 2017 | 28576360 |
| fusion of flagellin 2 with bivalent white spot syndrome virus vaccine increases survival in freshwater shrimp. | despite large economic losses attributable to white spot syndrome virus (wssv), an infectious pathogen of penaeid shrimp and other crustaceans worldwide, no efficient vaccines or antiviral agents to control the virus are available at present. here, we designed and constructed baculovirus-based vaccines delivering genes encoding the wssv envelope proteins, vp28 and vp19. to enhance the immunogenicity of the baculovirus-based vaccine, we fused a salmonella typhimurium flagellin 2 (fl2) gene with v ... | 2017 | 28216094 |
| the antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human hel 12469 cells. | six essential oils (from oregano, thyme, clove, lavender, clary sage, and arborvitae) exhibited different antibacterial and antifungal properties. antimicrobial activity was shown against pathogenic (escherichia coli, salmonella typhimurium, yersinia enterocolitica, staphylococcus aureus, listeria monocytogenes, and enterococcus faecalis) and environmental bacteria (bacillus cereus, arthrobacter protophormiae, pseudomonas fragi) and fungi (chaetomium globosum, penicillium chrysogenum, cladospori ... | 2017 | 28811611 |
| production of recombinant antimicrobial polymeric protein beta casein-e 50-52 and its antimicrobial synergistic effects assessment with thymol. | accelerating emergence of antimicrobial resistance among food pathogens and consumers' increasing demands for preservative-free foods are two contemporary challenging aspects within the food industry. antimicrobial packaging and the use of natural preservatives are promising solutions. in the present study, we used beta-casein-one of the primary self-assembly proteins in milk with a high polymeric film production capability-as a fusion partner for the recombinant expression of e 50-52 antimicrob ... | 2017 | 28561787 |
| edible wild plant heracleum pyrenaicum subsp. orsinii as a potential new source of bioactive essential oils. | many heracleum l. taxa (apiaceae) are used as food and spices, and in traditional medicine. in this work, the chemical composition of heracleum pyrenaicum subsp. orsinii (guss.) f. pedrotti and pignatti root, leaf and fruit essential oils, their antimicrobial activity and cytotoxic effect on malignant and normal cells were investigated for the first time. the composition of the oils was analyzed by gc and gc-ms. monoterpenes prevailed in the root oil, with β-pinene (38.6%) being dominant, while ... | 2017 | 28740275 |
| dietary safety of a dual-enzyme preparation for animal feed: acute and subchronic oral toxicity and genotoxicity studies. | animal feed is routinely supplemented with exogenous enzymes to improve nutrient utilization, such as proteases to enhance protein hydrolysis in vivo and xylanases to alleviate feed related anti-nutritional factors. the present studies were conducted to evaluate the potential oral toxicity and genotoxicity of a dual-enzyme preparation, vegpro(®) concentrate (vpr-c). acute oral toxicity studies were conducted in adult male and female sprague-dawley crl cd rats and chs swiss ico:ofi (iops caw) mic ... | 2017 | 28583667 |
| bio-mining the forest ecosystem of north east india for identification of antimicrobial metabolites from fungi through submerged fermentation. | in this study, fungi isolated from less explored forest soil ecosystem of northeast india were studied for the production of potential antimicrobial metabolites (amm). out of the 68 fungi isolated from forest soil of manipur, 7 of them showed ama against the test pathogens. among them, aspergillus terreus (ibsd-f4) showed the most significant activity against staphylococcus aureus (atcc-25923), bacillus anthracis (ibsd-c370), pseudomonas fluorescens (atcc-13525), salmonella typhimurium (atcc-140 ... | 2017 | 28578806 |
| synergistic antibacterial and antibiofilm efficacy of nisin in combination with p-coumaric acid against foodborne bacteria bacillus cereus and salmonella typhimurium. | the aim of the study was to evaluate possible antibacterial and antibiofilm efficacy of a bacteriocin, nisin with two essential oil components linalool and p-coumaric acid in combination against foodborne bacteria bacillus cereus and salmonella typhimurium. their inhibition effects on planktonic cells and pre-formed biofilms were evaluated using microbroth dilution and checkerboard titration methods. nisin/ p-coumaric acid combination showed synergistic effects against planktonic cells of both t ... | 2017 | 28815637 |
| phenolic acids and flavonoids of peanut by-products: antioxidant capacity and antimicrobial effects. | peanut skin (ps) and meal from dry-blanched peanuts (mdbp) were evaluated as sources of phenolic compounds. ps rendered the highest total phenolic content, antioxidant capacity towards abts radical cation, dpph and hydroxyl radicals as well as reducing power. phenolic acids were present in ps and mdbp whereas proanthocyanidins and monomeric flavonoids were found only in ps as identified by hplc-dad-esi-ms(n). procyanidin-rich extracts prevented oxidation in non-irradiated and gamma-irradiated fi ... | 2017 | 28764032 |
| isolation, morphological identification and in vitro antibacterial activity of endophytic bacteria isolated from azadirachta indica (neem) leaves. | the objective of this study was to isolate endophytic bacteria from azadirachta indica (neem) leaves, their identification and investigate their antibacterial activity against three gram-positive bacteria, staphylococcus aureus, streptococcus pyogenes and bacillus cereus and gram-negative bacteria escherichia coli, salmonella typhimurium and klebsiella pneumoniae. | 2017 | 28620254 |
| use of whey as a culture medium for bacillus clausii for the production of protein hydrolysates with antimicrobial and antioxidant activity. | the use of whey as a natural culture medium to produce hydrolysates with antimicrobial from bacillus clausii is the objective of this research. the fermentation process was carried out at 25 ℃ for five days, where proteolytic activity, soluble peptides quantification, and antimicrobial activity using the disc diffusion method were determined every day. the fermented whey reached values of proteolytic activity between 90 and 260 u ml(-1) and a production of soluble peptides between 100 and 2070 ... | 2017 | 28816527 |
| anti-biofilm effects of anthranilate on a broad range of bacteria. | anthranilate, one of tryptophan degradation products has been reported to interfere with biofilm formation by pseudomonas aeruginosa. here, we investigated the effects of anthranilate on biofilm formation by various bacteria and the mechanisms responsible. anthranilate commonly inhibited biofilm formation by p. aeruginosa, vibrio vulnificus, bacillus subtilis, salmonella enterica serovar typhimurium, and staphylococcus aureus, and disrupted biofilms preformed by these bacteria. because anthranil ... | 2017 | 28819217 |
| circulating and tissue-resident cd4+ t cells with reactivity to intestinal microbiota are abundant in healthy individuals and function is altered during inflammation. | interactions between commensal microbes and the immune system are tightly regulated and maintain intestinal homeostasis, but little is known about these interactions in humans. we investigated responses of human cd4(+) t cells to the intestinal microbiota. we measured the abundance of t cells in circulation and intestinal tissues that respond to intestinal microbes and determined their clonal diversity. we also assessed their functional phenotypes and effects on intestinal resident cell populati ... | 2017 | 28782508 |
| the probiotic combination of bifidobacterium longum subsp. infantis cect 7210 and bifidobacterium animalis subsp. lactis bpl6 reduces pathogen loads and improves gut health of weaned piglets orally challenged with salmonella typhimurium. | probiotics have been demonstrated to be useful to enhance gut health and prevent gastrointestinal infections in humans. additionally, some multi-strain probiotic combinations have been suggested to have greater efficacy than single strains. the objective of this study is to demonstrate the potential of a combination of the probiotic strains: bifidobacterium longum subsp. infantis cect 7210 (brand name b. infantis im1(®)) and b. animalis subsp. lactis bpl6 to enhance gut health and to ameliorate ... | 2017 | 28861074 |
| evaluation of the probiotic strain bifidobacterium longum subsp. infantis cect 7210 capacities to improve health status and fight digestive pathogens in a piglet model. | probiotics have been demonstrated to be useful to enhance gut health and prevent gastrointestinal infections. the objective of this study is to demonstrate the potential of the probiotic strain bifidobacterium longum subsp. infantis cect 7210 (b. infantis im1) to prevent and fight intestinal disease by using a salmonella typhimurium (trial 1) or an enterotoxigenic escherichia coli k88 (trial 2) oral challenge in a weaning piglet model. seventy-two piglets were used in each trial. after an adapta ... | 2017 | 28443068 |
| brucella lipopolysaccharide reinforced salmonella delivering brucella immunogens protects mice against virulent challenge. | intracellular pathogen salmonella exhibits natural infection broadly analogous to brucella, this phenomenon makes salmonella a pragmatic choice for an anti-brucella vaccine delivery platform. in this study we developed and formulated a combination of four attenuated salmonella typhimurium live vector strains delivering heterologous brucella antigens (rbs), namely lumazine synthase, proline racemase subunit a, lipoprotein outer membrane protein-19, and cu-zn superoxide dismutase. with an aim to d ... | 2017 | 28622869 |
| getting "inside" type i ifns: type i ifns in intracellular bacterial infections. | type i interferons represent a unique and complex group of cytokines, serving many purposes during innate and adaptive immunity. discovered in the context of viral infections, type i ifns are now known to have myriad effects in infectious and autoimmune disease settings. type i ifn signaling during bacterial infections is dependent on many factors including whether the infecting bacterium is intracellular or extracellular, as different signaling pathways are activated. as such, the repercussions ... | 2017 | 28529959 |
| survival of selected foodborne pathogens on dry cured pork loins. | the safety of ready-to-eat products such as cured pork loins must be guaranteed by the food industry. in the present study, the efficacy of the dry curing process of pork loins obtained from free-range pigs in the reduction of three of the most important foodborne pathogens is analysed. a total of 28 pork loin segments, with an average weight of 0.57±0.12kg, were divided into four groups with three being inoculated by immersion with 7logcfu/ml of either salmonella typhimurium, campylobacter coli ... | 2017 | 28759797 |
| postchill antimicrobial treatments to control salmonella, listeria, and campylobacter contamination on chicken skin used in ground chicken. | ground poultry products are frequently contaminated with foodborne pathogens. with the potential for increased regulatory scrutiny, it is important to use sufficient intervention strategies to control pathogen levels effectively. a large proportion of the bacteria introduced to ground chicken are likely to come from broiler skin, which is added to achieve target fat content and maintain product texture and taste. in this research, antimicrobials, including 50 ppm of chlorine and 1,200 ppm of per ... | 2017 | 28414258 |
| biosynthesized silver nanoparticles: are they effective antimicrobials? | silver nanoparticles (agnps) are increasingly being used in medical applications. therefore, cost effective and green methods for generating agnps are required. | 2017 | 28767978 |
| novel plasmid-mediated colistin resistance gene mcr-3 in escherichia coli. | the mobile colistin resistance gene mcr-1 has attracted global attention, as it heralds the breach of polymyxins, one of the last-resort antibiotics for the treatment of severe clinical infections caused by multidrug-resistant gram-negative bacteria. to date, six slightly different variants of mcr-1, and a second mobile colistin resistance gene, mcr-2, have been reported or annotated in the genbank database. here, we characterized a third mobile colistin resistance gene, mcr-3 the gene coexisted ... | 2017 | 28655818 |
| new insights into the roles of lpf and stg fimbriae in salmonella interactions with enterocytes and m cells. | salmonella enterica serovar typhi causes the systemic disease typhoid fever. after ingestion, it adheres to and invades the host epithelium while evading the host innate immune response, causing little if any inflammation. conversely, s typhimurium causes gastroenteritis in humans and thrives in the inflamed gut. upon entering the host, s typhimurium preferentially colonizes peyer's patches, a lymphoid organ in which microfold cells (m cells) overlay an arrangement of b cells, t cells, and antig ... | 2017 | 28630073 |
| in vitro characterization of lactobacillus plantarum strains with inhibitory activity on enteropathogens for use as potential animal probiotics. | the present study evaluates the probiotic properties of three lactobacillus plantarum strains mjm60319, mjm60298, and mjm60399 possessing antimicrobial activity against animal enteric pathogens. the three strains did not show bioamine production, mucinolytic and hemolytic activity and were susceptible to common antibiotics. the l. plantarum strains survived well in the simulated orogastrointestinal transit condition and showed adherence to caco-2 cells in vitro. the l. plantarum strains showed s ... | 2017 | 28611498 |
| a role for the tetraspanin proteins in salmonella infection of human macrophages. | infected macrophages play a role in the dissemination of salmonella and may serve as a reservoir of infection in asymptomatic carriers. however, relatively little is known about the early molecular interactions of the bacteria with these cells. we have recently shown that members of the tetraspanin family of membrane proteins are involved in the initial adhesion of a range of bacteria to host cells. this study investigated the role of tetraspanins in salmonella enterica subsp. enterica serovar t ... | 2017 | 28602860 |
| molecular subtyping of salmonella typhimurium with multiplex oligonucleotide ligation-pcr (mol-pcr). | a multiplex oligonucleotide ligation-pcr (mol-pcr) assay is a valuable high-throughput technique for the detection of bacteria and viruses, for characterization of pathogens and for diagnosis of genetic diseases, as it allows one to combine different types of molecular markers in a high-throughput multiplex assay. a mol-pcr assay starts with a multiplex oligonucleotide ligation reaction for detection of the molecular marker, followed by a singleplex pcr for signal amplification and analysis of t ... | 2017 | 28600761 |
| ca(2+) in hybridization solutions for fluorescence in situ hybridization facilitates the detection of enterobacteriaceae. | fluorescence in situ hybridization (fish) has been employed to identify microorganisms at the single cell level under a microscope. extensive efforts have been made to improve and extend the fish technique; however, the development of a widely applicable protocol is a continuing challenge. the present study evaluated the effects of divalent cations in the hybridization solution on the fish-based detection of various species of bacteria and archaea with rrna-targeted probes. a flow cytometric ana ... | 2017 | 28515389 |
| emergence of multidrug resistance in locally-acquired human infections with salmonella typhimurium in australia owing to a new clade harbouring blactx-m-9. | antimicrobial resistance in non-typhoidal salmonella is a critical problem globally, with the emergence of resistance to third-generation cephalosporins (3gcs) a particular concern. the aim of this study was to use whole-genome sequencing (wgs) to characterise recently identified human and non-human isolates of 3gc-resistant salmonella enterica subsp. enterica serovar typhimurium from australia. the illumina nextseq sequencing platform was used to determine the genome sequences of 78 s. typhimur ... | 2017 | 28476613 |
| draft genome sequences of salmonella enterica subsp. enterica serovar typhimurium strains isolated from chicken and swine carcasses in two distinct geographical regions from rio de janeiro state, brazil. | salmonella enterica subsp. enterica serovar typhimurium is a surveyed worldwide serotype with well-characterized genomes for several different strains. in brazil, very few studies have submitted whole-genome sequences to genbank. this genome may be useful to analyze the genetic mechanisms comparable to those of other related studies conducted in brazil and globally. | 2017 | 28450502 |
| sensitivity to antibiotics of bacteria exposed to gamma radiation emitted from hot soils of the high background radiation areas of ramsar, northern iran. | over the past several years our laboratories have investigated different aspects of the challenging issue of the alterations in bacterial susceptibility to antibiotics induced by physical stresses. | 2017 | 28432369 |
| the expanding targetome of small rnas in salmonella typhimurium. | the enterobacterial pathogen salmonella has long served as a model for bacterial pathogenesis, stress response, gene expression and regulation with extensive investigation involving protein function. with the advent of high-throughput sequencing technologies and their applications in genomics and transcriptomics, a wealth of data particularly with respect to small rnas (srnas) is being generated. these molecules serve as regulators of major stress response and virulence networks in diverse speci ... | 2017 | 28302471 |
| combined treatment with low concentrations of aqueous and gaseous chlorine dioxide inactivates escherichia coli o157:h7 and salmonella typhimurium inoculated on paprika. | combined treatment with gaseous and aqueous chlorine dioxide (clo2) was performed to improve the microbiological safety and quality of paprika. a single treatment of 50 ppmv clo2 gas for 30 min decreased the populations of escherichia coli o157:h7 and salmonella typhimurium by 2.33 and 2.91 log cfu/g, respectively. in addition, a single treatment of aqueous clo2 (50 ppm) for 5 min decreased these populations by 1.86 and 1.37, respectively. the most dramatic effects were achieved by combined trea ... | 2017 | 28035121 |
| impact of acanthamoeba cysts on stress resistance of salmonella enterica serovar typhimurium, yersinia enterocolitica 4/o:3, listeria monocytogenes 1/2a, and escherichia coli o:26. | the formation of robust resting cysts enables acanthamoeba to resist harsh environmental conditions. this study investigated to what extent these cysts are resistant to physical and chemical stresses as applied in food industry cleaning and disinfection procedures. moreover, it was assessed whether certain intracystic meat-borne bacterial pathogens are more stress resistant than free-living bacterial monocultures and if intracystic passage and subsequent association with trophozoites induces cro ... | 2017 | 28526786 |
| diversity of endophytic bacteria in a fern species dryopteris uniformis (makino) makino and evaluation of their antibacterial potential against five foodborne pathogenic bacteria. | the fern plant dryopteris uniformis has traditionally been used in herbal medicine and possesses many biological activities. this study was conducted to explore the endophytic bacterial diversity associated with d. uniformis and evaluate their antibacterial potential against foodborne pathogenic bacteria (fpb). among 51 isolated endophytic bacteria (eb), 26 eb were selected based on their morphological characteristics and identified by 16s rrna gene analysis. the distribution of eb was diverse i ... | 2017 | 28418717 |
| development and characterization of bioactive edible films from spider crab (maja crispata) chitosan incorporated with spirulina extract. | active food packaging films based on crab chitosan and spirulina extract (se) were developed. the effects of the se incorporation at different levels on physical (color, opacity water vapor and oxygen permeability) and mechanical (tensile strength and elongation at break) properties of chitosan films were investigated. ftir was carried out to observe the potential modifications of the chitosan films when incorporated with se. the obtained results suggested that incorporation of se into chitosan ... | 2017 | 28705500 |