Publications

matrix interference in serum total thyroxin (t4) time-resolved fluorescence immunoassay (trfia) and its elimination with the use of streptavidin-biotin separation technique.	in our development of total serum thyroxin trfia using an immobilized second-antibody (s-ab) as the separation agent, we observed a significant measurement bias caused by a matrix interference when the immobilized s-ab had a relatively low binding capacity for the primary anti-t4 monoclonal antibody (mcab). therefore, we employed a new separation system based on the highly active surface streptavidin and biotinylated anti-t4 mcab. our results indicate that the matrix interference was removed and ...	2001	11412823
advanced head and neck carcinoma in women: treatment outcomes may not improve with accelerated hyperfractionated radiotherapy.	the authors undertook a retrospective study on local tumor control, survival, and complications of conventional irradiation compared with accelerated hyperfractionated irradiation in women with selected head and neck tumor sites.	2001	11413525
desethylamiodarone antagonizes the effect of thyroid hormone at the molecular level.	to evaluate the molecular mechanisms of the inhibitory effects of amiodarone and its active metabolite, desethylamiodarone (dea) on thyroid hormone action.	2001	11415853
thyroid hormone regulates the obesity gene tub.	thyroid hormone t3/t4 is a major regulator of energy metabolism in vertebrates, and defects in thyroid status are frequently associated with changes in body weight. it is demonstrated here that thyroid hormone regulates in vivo and in vitro the tub gene, which when mutated in tubby mice causes obesity, insulin resistance and sensory deficits. hypothyroidism in rats altered tub mrna and protein in discrete brain areas. these changes could be attributed to thyroid hormone deficiency since t3/t4 tr ...	2001	11415982
related homing endonucleases i-bmoi and i-tevi use different strategies to cleave homologous recognition sites.	a typical homing endonuclease initiates mobility of its group i intron by recognizing dna both upstream and downstream of the intron insertion site of intronless alleles, preventing the endonuclease from binding and cleaving its own intron-containing allele. here, we describe a giy-yig family homing endonuclease, i-bmoi, that possesses an unusual recognition sequence, encompassing 1 base pair upstream but 38 base pairs downstream of the intron insertion site. i-bmoi binds intron-containing and i ...	2001	11416170
muscle enzyme adaptation to training and tapering-off in spinal-cord-injured humans.	the activity of muscle metabolic enzymes depends on the amount and type of physical training. we examined muscle enzyme adaptation to prolonged training followed by a period of lowered activity in spinal-cord-injured individuals (sci). ten sci [mean age 35 (sem 2) years, mean body mass 78 (sem 4) kg, mean time post-injury 12 (sem 2) years and range of lesion c5-t4] were given 12 months of functional electrical stimulation of an upright cycling motion for 30 min a day, three times a week, followe ...	2001	11417439
structure of a new polymorphic monoclinic form of human transthyretin at 3 a resolution reveals a mixed complex between unliganded and t4-bound tetramers of ttr.	the crystal structure of a new polymorphic form of human transthyretin (httr) with a lattice containing a unique assembly of apo httr and ttr-t(4) complex has been determined to 3 a resolution. the monoclinic form of human ttr reported here crystallizes in space group p2(1), with unit-cell parameters a = 76.7 (6), b = 96.7 (8), c = 81.7 (4) a, beta = 106.8 (4) degrees. the asymmetric unit contains two tetramers of transthyretin related by the non-crystallographic symmetry (ncs) operation of a 90 ...	2001	11418763
[hereditary thyroxin-binding globulin deficiency--changed thyroid function tests].	variation in concentrations of carrier proteins of hormones may influence the effect of the hormones and may cause confusion in the interpretation of laboratory results.	2001	11419101
a double chain reversal loop and two diagonal loops define the architecture of a unimolecular dna quadruplex containing a pair of stacked g(syn)-g(syn)-g(anti)-g(anti) tetrads flanked by a g-(t-t) triad and a t-t-t triple.	the architecture of g-g-g-g tetrad-aligned dna quadruplexes in monovalent cation solution is dependent on the directionality of the four strands, which in turn are defined by loop connectivities and the guanine syn/anti distribution along individual strands and within individual g-g-g-g tetrads. the smallest unimolecular g-quadruplex belongs to the d(g2nng2nng2nng2) family, which has the potential to form two stacked g-tetrads linked by nn loop connectivities. previous studies have focused on th ...	2001	11419945
early manifestations of "sick euthyroid" syndrome in patients with compensated chronic heart failure.	a "sick euthyroid" syndrome occurs in patients with severe decompensated chronic heart failure (chf) and other chronic illnesses and is related to adverse prognosis, but it has not been described in patients with compensated chf. the aim of this study was to determine whether manifestations of the sick euthyroid syndrome occur in patients with compensated chf caused by ischemic heart disease.	2001	11420766
semiquantitative evaluation of prevalent vertebral deformities in men and their relationship with osteoporosis: the minos study.	epidemiologic studies have shown a high prevalence of vertebral deformities in men without a steep increase with aging, suggesting that a substantial number of these deformities are not related to osteoporosis. to determine which vertebral deformities are likely to be osteoporotic fractures, we compared vertebral deformities and bone mineral density (bmd) in a cohort of 786 men aged 51-85 years (the minos study). normal vertebral height ratios were defined in a group of 120 healthy men aged 21-5 ...	2001	11420780
properties of bacteriophage t4 baseplate protein encoded by gene 8.	gene product 8 (gp8, 344 amino acids per monomer) of bacteriophage t4 is one of the baseplate structural proteins. we constructed an expression vector of gp8 and developed a method for purification of recombinant protein. cd spectroscopy showed that gp8 is an alpha/beta type structural protein. its polypeptide chain consists of nearly 40% beta-structure and 15% alpha-helix. these data agree with results of prediction of secondary structure based on the amino acid sequence of the protein. the sed ...	2001	11421820
an audit of management of differentiated thyroid cancer in specialist and non-specialist clinic settings.	thyroid cancer is the most common endocrine malignancy but is none the less rare. some aspects of its management remain controversial. previous audits of patient management in the united kingdom have revealed deficiencies, especially in communication between specialists. we have audited patient management in a large university-associated teaching hospital, assessing points of good practice identified from published guidelines and reviews, and have compared findings in groups of patients managed ...	2001	11422105
ratio of invaded to removed lymph nodes as a prognostic factor in adenocarcinoma of the distal esophagus and esophagogastric junction.	this study examined the influence of nodal harvest and the proportion of positive nodes on survival in 59 patients with adenocarcinoma of the distal esophagus and esophagogastric junction undergoing esophagectomy with curative intent. a total of 754 lymph nodes were harvested (median 13, range 0-28). two hundred and twenty-eight positive nodes were found on histology (median 4, range 1-23) in 43 (79%) patients with a higher incidence from t3/t4 than t1/t2 lesions (p < 0.003). overall 1- and 3-ye ...	2001	11422303
[intradural spinal arachnoid cyst associated with noonan's syndrome].	intradural spinal arachnoid cysts are collections of liquid similar to csf, caused by a disorder of the arachnoid trabeculae. noonan s syndrome is a condition involving multiple malformations, with autosomal dominant inheritance, variable penetration and expression and a phenotype similar to that of turner s syndrome. we describe the case of a girl with noonan s syndrome who presented with progressive scoliosis and signs and symptoms of a disorder of the spine related to an intradural spinal ara ...	2001	11424034
nasopharyngeal carcinoma in turkish children: review of 33 cases.	a retrospective and prospective analysis is reported of epidemiological, clinical, and therapeutic aspects of 33 children with nasopharyngeal carcinoma who were treated in a single institution over a period of 10 years. twenty-three male and 10 female children ranging from 9 to 17 years were referred to our center. histopathology was who type 3 carcinoma in 21, who type 2 in 8, who type 1 in 1, and unclassified in 3 patients. disease extent was t2a (n = 15), t2b (n = 2), t3 (n = 11), and t4 (n = ...	2001	11452402
11beta-hydroxysteroid dehydrogenase bioactivity is increased in the colon but not kidneys of rats given supplementary thyroxine.	the aim of this study was to investigate the effect of altered thyroid status on 11beta-hydroxysteroid dehydrogenase type 1 (11beta-hsd type 1) and type 2 (11beta-hsd type 2) bioactivity in rat kidney and colon. male sprague-dawley rats (250 g) were treated with either l-thyroxine (t4) or propylthiouracil (ptu) for 4 weeks. blood were then analysed for serum thyroxine, sodium (na+) and potassium (k+). the kidneys and colon were assayed for 11beta-hsd type 1 and 11beta-hsd type 2 bioactivity. in ...	2001	11453035
thyroid function testing in greyhounds.	to evaluate thyroid function in healthy greyhounds, compared with healthy non-greyhound pet dogs, and to establish appropriate reference range values for greyhounds.	2001	11453491
thyroid function in survivors of childhood acute lymphoblastic leukaemia: the significance of prophylactic cranial irradiation.	focus on long-term side-effects after cancer therapy in childhood has become of the utmost importance. the hypothalamic-pituitary thyroid (hpt) axis is exposed to irradiation when some children are treated for acute lymphoblastic leukaemia (all) with prophylactic cranial irradiation (cir). whether this treatment causes hypofunction of the hpt axis remains controversial.	2001	11453948
hyperthyroidism is associated with lengthening of ventricular repolarization.	lengthened ventricular repolarization, as assessed by the qt interval on electrocardiogram (ecg), can predispose to an increased risk of cardiac dysrhythmias; no data are available on qt corrected for heart rate (qtc) in hyperthyroidism in vivo.	2001	11453949
the nature of human chorionic gonadotrophin glycoforms in gestational thyrotoxicosis.	we examined the charge heterogeneity and carbohydrate complexity of hcg in healthy pregnant asian subjects and asian women with gestational thyrotoxicosis to assess whether particular glycoforms of hcg are associated with the thyrotoxicosis of pregnancy.	2001	11453950
differential effect of hyperthyroidism on rat epididymal glycosidases.	the impact of hyperthyroidism on epididymal glycosidases was studied in albino rats. hyperthyroidism was induced in wistar rats aged 30 days by daily injection of t4 (25 microg/100 g body weight/day intramuscularly) for 30 or 60 days; control rats were injected with vehicle (alkaline saline, ph 7.8). one set of hyperthyroid rats was reverted to euthyroid status by withdrawing t4 treatment after 30 days of hyperthyroidism. to asses the direct effect of thyroid hormone on epididymal hexosaminidase ...	2001	11454072
graves' disease presenting as elephantiasic pretibial myxedema and nodules of the hands.	a 67-year-old man presented with a 2-year history of asymptomatic, firm, multiple nodules and plaques and cerebriform hypertrophy of both lower legs and feet, and well-defined, skin-colored, firm nodules and tumors on both hands. he had been diagnosed as having graves' disease 3 years previously, and had been treated with 10 mg of methimazole and 100 microg of thyroxin (t4) daily for 2 years. physical examination revealed nonpitting edema, flesh-colored to erythematous, firm, confluent, polypoid ...	2001	11454085
tetra-o-methyl nordihydroguaiaretic acid induces g2 arrest in mammalian cells and exhibits tumoricidal activity in vivo.	the transcription inhibitor tetra-o-methyl nordihydroguaiaretic acid (m4n) was found to arrest the proliferation of c3, c33a, cem-t4, and tc-1 cells in culture at the g2 stage of the cell cycle. investigation into the mechanism of arrest revealed that m4n reduces mrna levels and subsequent protein production of the cyclin-dependent kinase cdc2, resulting in the inactivation of the cdc2/cyclin b complex (maturation promoting factor). when injected intratumorally in a c3-cell induced c57bl/6 mouse ...	2001	11454698
dynamics of translesion dna synthesis catalyzed by the bacteriophage t4 exonuclease-deficient dna polymerase.	the mechanism and dynamics of translesion dna synthesis were evaluated using primer/templates containing a tetrahydrofuran moiety designed to mimic an abasic site. steady-state kinetic analysis reveals that the t4 dna polymerase preferentially incorporates datp across from the abasic site with 100-fold higher efficiency than the other nucleoside triphosphates. under steady-state conditions, the catalytic efficiency of datp incorporation across from an abasic site is only 220-fold lower than that ...	2001	11401565
[value of a second transurethral resection in the assessment and treatment of patients with bladder tumor].	to analyse the value of a second transurethral resection, repeated within 3 to 6 weeks after the initial resection, in the treatment and the classification of patients with bladder tumour.	2001	11402530
intracavitary afterloading boost in anal canal carcinoma. results, function and quality of life.	first clinical data on a new intracavitary afterloading boost method for anal canal carcinoma is reported.	2001	11398611
dissection of the atp-driven reaction cycle of the bacteriophage t4 dna replication processivity clamp loading system.	processive dna replication requires the loading of a multisubunit ring-shaped protein complex, known as a sliding or processivity clamp, onto the primer-template (p/t) dna. this clamp then binds to the replication polymerase to form a processive polymerase holoenzyme. the processivity of the holoenzyme derives from the topological properties of the clamp, which encircles the dna without actually binding to it. multisubunit complexes known as clamp-loaders utilize atp to drive the placement of th ...	2001	11399065
analysis of the roles of trna structure, ribosomal protein l9, and the bacteriophage t4 gene 60 bypassing signals during ribosome slippage on mrna.	a 50-nucleotide coding gap divides bacteriophage t4 gene 60 into two open reading frames. in response to cis-acting stimulatory signals encrypted in the mrna, the anticodon of the ribosome-bound peptidyl trna dissociates from a gga codon at the end of the first open reading frame and pairs with a gga codon 47 nucleotides downstream just before the second open reading frame. mutations affecting ribosomal protein l9 or trna(gly)(2), the trna that decodes gga, alter the efficiency of bypassing. to ...	2001	11399077
entry of thyroid hormones into tilapia oocytes.	the patterns of entry of thyroid hormones into live tilapia oocytes were examined by incubating ovarian follicles in l-15 medium containing 125i-labeled thyroxine (t4) or 3,5,3'-triiodothyronine (t3). as judged from hplc profiles, radioactivity in extracts of follicles immersed in t3 was identified to reside in t3, while most of the radioactivity in the extract of t4 immersed follicle was not associated with t4. radioactivity of t3 immersed follicles reached a constant level after 18 h of incuba ...	2001	11399496
the significance of comorbidity in advanced laryngeal cancer.	cancer patients often have concurrent diseases and conditions known as comorbidities. the aim of this project is to demonstrate the significance of comorbidity in the treatment and outcomes of advanced laryngeal carcinoma.	2001	11400245
discrimination of anesthetic states using mid-latency auditory evoked potential and artificial neural networks.	this study was undertaken to determine whether artificial neural network (ann) processing of mid-latency auditory evoked potentials (mlaeps) can identify different anesthetic states during propofol anesthesia, and to determine those parameters that are most useful in the identification process. twenty-one patients undergoing elective abdominal surgery were studied. to maintain general anesthesia, the patients received propofol (3-5 mgkg(-1) h(-1) intravenously). epidural analgesia at the level o ...	2001	11400725
the effect of methimazole pretreatment on the efficacy of radioactive iodine therapy in graves' hyperthyroidism: one-year follow-up of a prospective, randomized study.	the effect of antithyroid drugs on the efficacy of radioiodine (131i) treatment is still controversial. this study evaluated the effect of methimazole pretreatment on the efficacy of 131i therapy in graves' hyperthyroidism. sixty-one untreated patients were randomly assigned to receive 131i alone (32 patients) or 131i plus pretreatment with methimazole (30 mg/d; 29 patients). 131i was administered 4 d after drug discontinuation. the calculated 131i dose was 200 microci/g thyroid tissue as estima ...	2001	11502768
131i therapy for differentiated thyroid cancer leads to an earlier onset of menopause: results of a retrospective study.	treatment with 131i for differentiated thyroid cancer may give a follicle-damaging radiation dose to the ovaries. this damage to the ovarian function could shorten the fertile life span and advance the natural menopause. to address this issue, we studied retrospectively the menopausal age of 130 women treated with 131i for differentiated thyroid cancer in our institution from 1974-1993. the menopausal age of women treated with 131i for differentiated thyroid cancer after total thyroidectomy and ...	2001	11502772
thyroid function and autoimmunity during interferon beta-1b treatment: a multicenter prospective study.	thyroid dysfunction and autoimmunity have been reported during type i interferon therapy, namely interferon-alpha for chronic hepatitis or interferon-beta for multiple sclerosis. to define the frequency of thyroid dysfunction and autoimmunity during interferon-beta treatment, 156 multiple sclerosis patients were prospectively followed up by 18 centers for 1 yr after starting interferon-beta-1b treatment. serial clinical assessments and tests of thyroid and liver function and antithyroid autoanti ...	2001	11502774
radioiodine treatment of hyperthyroidism-prognostic factors for outcome.	there is little consensus regarding the most appropriate dose regimen for radioiodine (131i) in the treatment of hyperthyroidism. we audited 813 consecutive hyperthyroid patients treated with radioiodine to compare the efficacy of 2 fixed-dose regimens used within our center (185 megabequerels, 370 megabequerels) and to explore factors that may predict outcome. patients were categorized into 3 diagnostic groups: graves' disease, toxic nodular goiter, and hyperthyroidism of indeterminate etiology ...	2001	11502786
building a replisome solution structure by elucidation of protein-protein interactions in the bacteriophage t4 dna polymerase holoenzyme.	assembly of dna replication systems requires the coordinated actions of many proteins. the multiprotein complexes formed as intermediates on the pathway to the final dna polymerase holoenzyme have been shown to have distinct structures relative to the ground-state structures of the individual proteins. by using a variety of solution-phase techniques, we have elucidated additional information about the solution structure of the bacteriophage t4 holoenzyme. photocross-linking and mass spectrometry ...	2001	11504721
initiation of dna replication by dna polymerases from primers forming a triple helix.	despite extensive studies on oligonucleotide-forming triple helices, which were discovered in 1957, their possible relevance in the initiation of dna replication remains unknown. using sequences forming triple helices, we have developed a dna polymerisation assay by using hairpin dna templates with a 3' dideoxynucleotide end and an unpaired 5'-end extension to be replicated. the t7 dna polymerase successfully elongated nucleotides to the expected size of the template from the primers forming tri ...	2001	11504869
target-controlled infusion or manually controlled infusion of propofol in high-risk patients with severely reduced left ventricular function.	to compare hemodynamics, time to extubation, and costs of target-controlled infusion (tci) with manually controlled infusion (mci) of propofol in high-risk cardiac surgery patients.	2001	11505347
results of salvage surgery for local or regional recurrence after larynx preservation with induction chemotherapy and radiotherapy.	after treatment of locally advanced laryngeal carcinomas with induction chemotherapy and radiotherapy, some patients suffer a local or regional failure of the tumor, and salvage surgery is required. the aim of this study was to review the results of such salvage surgery in this group of patients.	2001	11505482
cancer of retromolar trigone: long-term radiation therapy outcome.	cancer of the retromolar trigone is an uncommon head and neck cancer. in this retrospective study, we identified the prognostic factors and evaluated the therapeutic outcomes of patients treated with preoperative radiation therapy (rt), postoperative rt, and rt alone.	2001	11505486
[controversy over the uicc-tnm classification].	the new uicc-tnm classification is accepted by most thoracic surgeons and medical oncologists because the prognosis of lung cancer patients is well distinguished by stage based on the tnm classification. however, there are several controversies over improving the classification. the number of small-sized peripheral lung cancers detected by helical computed tomography screening is rapidly increasing in japan. the prognosis for patients with these tiny lung cancers is extremely good. therefore, th ...	2001	11505500
[surgery for non-small cell lung cancer based on the t factor].	we review the indications of surgery in patients with non-small cell lung cancer (nsclc) based on the t factor, focusing on peripheral small tumors, invasion to other organs, and the presence of malignant pleural effusion or intrapulmonary metastasis. while limited surgery in patients with peripheral, small-sized nsclc preserves postoperative pulmonary function, the prospects for long-term survival are reduced due to the likelihood of recurrence, novel prospective studies are being conducted to ...	2001	11505501
[bicalutamide monotherapy of patients with disseminated forms of prostatic cancer].	combined antiandrogen therapy (complete androgen blocking) implies surgical or chemical castration in combination with antiandrogen therapy. both sources of androgens are thus blocked in patients with locally disseminated prostatic cancer. the purpose of this study was to evaluate the efficiency of bikalutamide (150 mg) monotherapy. the study was carried out in 58 patients with prostatic cancer (stages t3-t4) divided into 2 groups: 1) bikalutamide monotherapy (150 mg daily) and 2) bikalutamide ( ...	2001	11505537
a subclass of her1 ligands are prognostic markers for survival in bladder cancer patients.	members of the epidermal growth factor (egf) family have been suggested as prognostic markers in patients with bladder cancer. thus far, there has been no consensus on their usefulness. we report an analysis of six ligands and two receptors of which a subset correlate to tumor stage and survival. biopsies from bladder cancer tumors were obtained from 73 patients followed for a median of 28 months. the mrna content for six ligands [egf, transforming growth factor alpha (tgf-alpha), amphiregulin ( ...	2001	11507076
[distribution of polycyclic aromatic hydrocarbon in core sediments of the bohai straight].	thirteen compounds of pahs were identified by gc in the core sediments of bohai straight (s44 and t4 stations). the distribution and source identification were studied with the two core sediments. the total concentrations changed from 60.3 ng/g to 2076.5 ng/g. though the contamination of pahs in the study area is quite modest, some carcinogenic compounds were detected in most samples. from the indicators such as pen/anth and fluo/pyr etc. the dominated origin of pahs in s44 station is pyrolytic ...	2001	11507912
the effects of thyroxine isomers on free-radical oxidation processes in subcellular fractions of rat cerebral cortex.	measurements made by chemiluminescence (cl) were used to estimate effective d- and l-thyroxine concentrations and to study their effects on free-radical oxidation processes in the mitochondrial and synaptosomal fractions of rat cerebral cortex in vitro. these experiments showed that in a model system containing riboflavin, the antioxidant activity of d-t4 was 2.2 times greater than that of l-t4. the effective concentrations for the two forms of thyroxine were i50 = 74.3 +/- 7.1 microm for d-t4 a ...	2001	11508500
gastric emptying and gastrointestinal transit of liquid throughout the first month after thoracic spinal cord transection in awake rats.	spinal cord transection (sct) inhibits gastrointestinal motility in awake rats. we studied the gastric emptying (ge) and gastrointestinal transit of liquid throughout the first month after thoracic sct. male wistar rats (n = 66) were submitted to laminectomy followed or not by complete sct between t4 and t5 vertebrae. phenol red recovery in the stomach, proximal, mid-and distal small intestine was determined 1, 7, 10, 15, and 30 days thereafter. gastric recovery increased by 51.2 and 38.9% and m ...	2001	11508656
review: molecular thyroidology.	novel disorders involving aberrations of the hypothalamic-pituitary-thyroid gland-thyroid hormone axis have been described in the last 5 to 10 years. the following topics are addressed: molecular mutations causing central hypothyroidism (isolated autosomal recessive trh deficiency; autosomal recessive trh-receptor inactivating mutations; tsh beta-subunit bio-inactivating mutations; pit-1 mutations; prop1 mutations; high molecular weight bio-inactive tsh); defects in response to tsh (mutations in ...	2001	11508826
decreased levels of ionized calcium one year after hemithyroidectomy: importance of reduced thyroid hormones.	previously we have found reduced levels of total serum calcium and 1,25(oh)2d3 despite an unaltered stimulated parathyroid hormone (pth) secretion 1 year after hemithyroidectomy. the present study was undertaken to elucidate the possible relationship between calcium homeostasis, thyroid hormones and bone resorption in a group of 45 consecutive patients subjected to hemithyroidectomy because of a solitary nodule. all patients had free t4 and t3 levels within normal range preoperatively.	2001	11509864
the effects of vasopressin on hemodynamics and renal function in severe septic shock: a case series.	to review all cases of septic shock treated with vasopressin to determine the effects on hemodynamic and renal function and to document any adverse effects.	2001	11511958
[cancer of the prostate in france: results of the survey ccafu-francim].	the management, diagnosis and treatment of prostate cancer (pc) in the general population are poorly defined in france. the objective of this survey was to analyse the diagnostic and therapeutic modalities of prostate cancer in 1995, on the basis of a population derived from 4 french cancer registries.	2001	11512461
role of medium- and long-range interactions to the stability of the mutants of t4 lysozyme.	inter-residue interactions play an important role to the folding and stability of protein molecules. in this work, we analyze the role of medium- and long-range interactions to the stability of t4 lysozyme mutants. we found that, in buried mutations, the increase in long-range contacts upon mutations destabilizes the protein, whereas, in surface mutations, the increase in long-range contacts increases the stability, indicating the importance of surrounding polar residues to the stability of surf ...	2001	11513088
sites of nucleic acid binding in type i-iv intermediate filament subunit proteins.	a combination of enzymatic and chemical ladder sequencing of photo-cross-linked protein-single-stranded oligodeoxyribonucleotide complexes and analysis by maldi-tof mass spectrometry was employed to identify the amino acid residues responsible for the stable binding of nucleic acids in several intermediate filament (if) subunit proteins. the if proteins studied included the type i and type ii cytokeratins k8, k18, and k19; the type iii proteins desmin, glial fibrillary acidic protein (gfap), per ...	2001	11513613
is the infertility in hypothyroidism mainly due to ovarian or pituitary functional changes?	the objective of the present study was to examine whether hypothyroidism affects the reproductive system of adult female rats by evaluating ovarian morphology, uterus weight and the changes in serum and pituitary concentrations of prolactin and gonadotropins. three-month-old female rats were divided into three groups: control (n = 10), hypothyroid (n = 10), treated with 0.05% 6-propyl-2-thiouracil (ptu) in drinking water for 60 days, and t4-treated group (n = 10), receiving daily sc injections o ...	2001	11514846
t4 rectal cancer treated with preoperative chemoradiation to the posterior pelvis followed by multivisceral resection: patterns of failure and limitations of treatment.	to analyze the overall pattern of treatment failure and sites of pelvic disease recurrence relative to the radiation fields used in treating patients with clinically staged t4 rectal cancer with preoperative chemoradiation followed by multivisceral resection.	2001	11516868
conserved regions 4.1 and 4.2 of sigma(70) constitute the recognition sites for the anti-sigma factor asia, and asia is a dimer free in solution.	the association of the bacteriophage t4-encoded asia protein with the final sigma(70) subunit of the escherichia coli rna polymerase is one of the principal events governing transcription of the t4 genome. analytical ultracentrifugation and nmr studies indicate that free asia is a symmetric dimer and the dimers can exchange subunits. using nmr, the mutual recognition sites on asia and final sigma(70) have been elucidated. residues throughout the n-terminal half of asia are involved either direct ...	2001	11518715
[effects of working conditions in the production of synthetic rubber on workers' health].	examination of 325 kazan workers engaged in the production of synthetic rubber revealed a high (35.6%) prevalence of thyroid pathology. patients with thyroid disease and apparently healthy workers manufacturing synthetic rubber were found to have lower levels of t3, t4, and zinc and higher levels of thyroglobulin antibodies and chromium in the serum than in apparently healthy individuals not contacting with noxious agents. the magnitude of increases in the workers' serum concentration of chromiu ...	2001	11519462
the activity of antioxidant enzymes and the content of uncoupling protein-1 in the brown adipose tissue of hypothyroid rats: comparison with effects of iopanoic acid.	the activity of antioxidant enzymes, copper-zinc superoxide dismutase (cuznsod), manganese superoxide dismutase (mnsod) and catalase (cat), as well as that of the mitochondrial fad-dependent alpha-glycerophosphate dehydrogenase (alpha-gpd) in the rat interscapular brown adipose tissue (ibat) were studied after the treatment with methimazole (mmi) for three weeks or with iopanoic acid (iop) for five days. besides, the mitochondrial concentration of uncoupling protein-1 (ucp-1) and the activity of ...	2001	11521740
a phase i-ii study of synchronous chemoradiotherapy for poor prognosis locally advanced bladder cancer.	the management of locally advanced bladder cancer remains controversial with poor local control with radiotherapy alone. synchronous chemotherapy regimens have yielded encouraging results in other primary sites.	2001	11521797
trabecular shear stress in human vertebral cancellous bone: intra- and inter-individual variations.	correlation of the mean and standard deviation of trabecular stresses has been proposed as a mechanism by which a strong relationship between the apparent strength and stiffness of cancellous bone can be achieved. the current study examined whether the relationship between the mean and standard deviation of trabecular von mises stresses can be generalized for any group of cancellous bone. cylindrical human vertebral cancellous bone specimens were cut in the infero-superior direction from t12 of ...	2001	11522314
a new mode of percutaneous upper thoracic phenol sympathicolysis: report of 50 cases.	our previous study demonstrated that a high concentration of phenol (75-90%) with minimal volume (0.02 ml) can elicit serious degeneration of ganglion cells of the stellate ganglia in cats. another previous study in our clinical patients demonstrated that approximately 84 to 90% of the upper thoracic (t2-t3) sympathetic trunks can be found under an endoscope on the ventral side of the t2-t3 rib heads. in this report, we present a new mode of dorsal percutaneous thoracic phenol sympathicolysis (p ...	2001	11523673
base sequence effects in bending induced by bulky carcinogen-dna adducts: experimental and computational analysis.	the covalent binding of bulky mutagenic or carcinogenic compounds to dna can lead to bending, which could significantly alter the interactions of dna with critical replication and transcription proteins. the impact of adducts derived from the highly reactive bay region enantiomeric (+)- and (-)-anti-7,8-diol-9,10-epoxide derivatives of benzo[a]pyrene (bpde) are of interest because the (+)-7r,8s,9s,10r-anti-bpde enantiomer is highly tumorigenic in rodents, while the (-)-7s,8r,9r,10s-anti-bpde ena ...	2001	11523987
[evaluation of a combination chemotherapy with nedaplatin and 5-fu for oral cancers].	nedaplatin (cis-diammine-glycolato platinum: cdgp) is a platinum compound with a molecular weight of 303.18 that was recently developed in japan. there have been reports of the antineoplastic effects of nedaplatin on cancers in the cranio-cervical region, lung, esophagus, urinary bladder, testis, ovary, and uterus. in this study, we performed combined therapy of cdgp and fluorouracil (5-fu) for 8 patients with oral cancers, and evaluated the results to elucidate the clinical effect and adverse s ...	2001	11525027
lack of substantial effects of raloxifene on thyroxine-binding globulin in postmenopausal women: dependency on thyroid status.	long-term estrogen therapy can modify thyroid hormone kinetics by increasing serum concentration of thyroxine-binding globulin (tbg). raloxifene is a recently developed selective estrogen receptor modulator (serm) for the treatment of osteoporosis, which possesses estrogenic and antiestrogenic properties. in a prospective and randomized study, we investigated the effects of raloxifene on tbg levels and on the serum concentrations of free thyroxine (ft4), thyroxine (t4), triiodothyronine (t3), an ...	2001	11525272
predictors of survival for esophageal cancer patients with and without celiac axis lymphadenopathy: impact of staging endosonography.	esophageal cancer patients with m1a disease are reported to have poor survival. we hypothesized that patients with celiac lymph node metastases (cln) identified by endoscopic ultrasonography (eus) would predict a cohort with significantly worse survival postoperatively. accurate preoperative identification of this group will facilitate future adjuvant studies.	2001	11465182
3'-5' exonucleolytic activity of dna polymerases: structural features that allow kinetic discrimination between ribo- and deoxyribonucleotide residues.	we have determined rates for the excision of nucleotides from the 3' termini of chimeric dna-rna oligonucleotides using the klenow fragment (kf) and two other dna polymerases, from phages t4 and t7. for these studies, we synthesized dna-rna chimeric oligonucleotides with rna residues in defined positions. when a ribonucleotide residue was placed at the 3' terminus, all three dna polymerases removed it at the same rate as they did for substrates composed solely of deoxynucleotide residues. there ...	2001	11467934
direct and indirect free thyroxine assay methods: theory and practice.	for the diagnosis of thyroid disease, measurement of "free hormone" is generally accepted as an appropriate measure. however, valid assays measuring the free fraction of thyroxine (ft4) ideally must perform without bias, despite large variations in the concentrations and affinities of serum t4-binding proteins in the population. several approaches have been taken to overcome such bias, and these have created considerable controversy in the field over the past decade.	2001	11468222
ramification amplification: a novel isothermal dna amplification method.	we have developed a novel isothermal dna amplification method with an amplification mechanism quite different from conventional pcr. this method uses a specially designed circular probe (c-probe) in which the 3' and 5' ends are brought together in juxtaposition by hybridization to a target. the two ends are then covalently linked by a t4 dna ligase in a target-dependent manner, producing a closed dna circle. in the presence of an excess of primers (forward and reverse primers), a dna polymerase ...	2001	11468700
physiopathological effects of the administration of chronic cyanide to growing goats--a model for ingestion of cyanogenic plants.	ingestion of cyanogenic plants, such as cassava and sorghum, has been associated with goitre and tropical pancreatic diabetes in both humans and animals. thus, the objective of the present study was to determine the toxic effects on the thyroid and pancreas in growing goats of prolonged exposure to potassium cyanide (kcn). thirty-four male goats were divided into five groups dosed with kcn at 0 (control). 0.3, 0.6, 1.2 or 3.0 mg/kg daily for 5 months. blood samples were obtained in order to dete ...	2001	11469509
association between weight gain, blood parameters, and thyroid hormones and the development of ascites syndrome in broiler chickens.	the present study examined the association between thyroid hormones and the development of ascites on one hand and the ability to predict ascites from growth rate and hematocrit on the other hand. ascites syndrome was induced in broiler chickens in two trials by exposing the chicks to low ambient temperature (ta) and by supplying a pellet form of diet. weight gain, hematocrit, hemoglobin, and plasma thyroxin (t4) and triiodothyronine (t3) concentrations were measured weekly for each bird, and co ...	2001	11469663
the distribution patterns of trace elements in the brain and erythrocytes in a rat experimental model of iodine deficiency.	cretinism is a disease characterized by neurological defects associated with severe iodine deficiency. in a rat model of severe iodine deficiency, we investigated the distribution pattern of trace elements (iodine [i], selenium [se], and bromine [br] in brain tissue samples; potassium [k], calcium [ca], manganese [mn], iron [fe], copper [cu], zinc [zn], rubidium [rb], and lead [pb] in erythrocytes) after supplementing the rats with i and/or se. neutron activation analysis, proton induced x-ray e ...	2001	11470332
structure of the udp-glucosyltransferase gtfb that modifies the heptapeptide aglycone in the biosynthesis of vancomycin group antibiotics.	members of the vancomycin group of glycopeptide antibiotics have an oxidatively crosslinked heptapeptide scaffold decorated at the hydroxyl groups of 4-oh-phegly4 or beta-oh-tyr6 with mono- (residue 6) or disaccharides (residue 4). the disaccharide in vancomycin itself is l-vancosamine-1,2-glucose, and in chloroeremomycin it is l-4-epi-vancosamine-1,2-glucose. the sugars and their substituents play an important role in efficacy, particularly against vancomycin-resistant pathogenic enterococci.	2001	11470430
reference intervals for thyroid hormones in pregnant chinese women.	to establish gestation-related reference intervals for thyroid hormones in a chinese population.	2001	11471873
synthesis and characterization of dna duplexes containing an n(4)c-ethyl-n(4)c interstrand cross-link.	short dna duplexes containing an n(4)c-ethyl-n(4)c interstrand cross-link, c-c, were synthesized on controlled pore glass supports. duplexes having two, three, or four a/t base pairs on either side of the c-c cross-link and terminating with a c(4) overhang at their 5'-ends were prepared. the cross-link was introduced using a convertible nucleoside approach. thus, an oligonucleotide terminating at its 5'-end with o(4)-triazoyl-2'-deoxyuridine was first prepared on the support. the triazole group ...	2001	11472110
a multicenter evaluation of the time-course of action of two doses of rapacuronium after early and late reversal with neostigmine.	early reversal of rapacuronium may accelerate return of neuromuscular function. this study was designed to compare early (2 min after rapacuronium) or late (at 25% recovery of the first twitch [t1] of train-of-four) reversal of rapacuronium with neostigmine. we studied 119 subjects between the ages of 18 and 75 yr. anesthesia was induced with fentanyl and thiopental and maintained with nitrous oxide, propofol, and fentanyl. mechanomyographic neuromuscular monitoring was performed by using train- ...	2001	11473863
colloids versus crystalloids and tissue oxygen tension in patients undergoing major abdominal surgery.	the effects of intravascular volume replacement regimens on tissue oxygen tension (ptio(2)) are not definitely known. forty-two consecutive patients scheduled for elective major abdominal surgery were prospectively randomized to receive either 6% hydroxyethyl starch (hes) (mean molecular weight 130 kd, degree of substitution 0.4, n = 21) or lactated ringer's solution (rl, n = 21) for intravascular volume replacement. fluids were administered perioperatively and continued for 24 h on the intensiv ...	2001	11473870
inflammatory response, growth, and thyroid hormone concentrations are affected by long-term boron supplementation in gilts.	an experiment was conducted to determine the long-term effects of dietary boron (b) on growth performance, immune function, and plasma and serum characteristics in gilts. fifty weanling gilts were allotted to 10 pens based on weaning weight and litter origin. pens were randomly assigned to receive one of two dietary treatments. treatments consisted of a basal diet low in b (control) and the basal diet supplemented with 5 mg b/kg diet as sodium borate. gilts remained on their respective experimen ...	2001	11424693
the emergence of erythromycin-resistant streptococcus pyogenes in seoul, korea.	high frequencies of erythromycin-resistant streptococci were reported in japan in the mid-1970s, and in finland in the late 1980s, related to an increase in the consumption of macrolide antibiotics in these countries. the frequency of erythromycin-resistant streptococcus pyogenes was reported to be only 2% in 1994, but we know that the susceptibility of the strains to antibiotics had not been tested routinely. we studied the resistance rates of streptococcus pyogenes to various antibiotics in se ...	2001	11455497
measurement of slow (micros-ms) time scale dynamics in protein side chains by (15)n relaxation dispersion nmr spectroscopy: application to asn and gln residues in a cavity mutant of t4 lysozyme.	a new nmr experiment is presented for the measurement of micros-ms time scale dynamics of asn and gln side chains in proteins. exchange contributions to the (15)n line widths of side chain residues are determined via a relaxation dispersion experiment in which the effective nitrogen transverse relaxation rate is measured as a function of the number of refocusing pulses in constant-time, variable spacing cpmg intervals. the evolution of magnetization from scalar couplings and dipole-dipole cross- ...	2001	11456632
ti location in the mfi framework of ti-silicalite-1: a neutron powder diffraction study.	the first direct evidence that ti atoms are not equally distributed in the 12 crystallographically independent t sites of the mfi framework is presented on the basis of neutron diffraction data collected at the hrpd instrument of the isis pulsed neutron source. we found strong evidence indicating that t6, t7, and t11 are the most populated sites and weak evidence that ti may be hosted in t10. ti occupancy can be excluded for sites t1, t2, t4, t5, t9, and t12. the occupancy of the remaining sites ...	2001	11456866
probing slow time scale dynamics at methyl-containing side chains in proteins by relaxation dispersion nmr measurements: application to methionine residues in a cavity mutant of t4 lysozyme.	a relaxation dispersion-based nmr experiment is presented for the measurement and quantitation of micros-ms dynamic processes at methyl side-chain positions in proteins. the experiment measures the exchange contribution to the 13c line widths of methyl groups using a constant-time cpmg scheme. the effects of cross-correlated spin relaxation between dipole-dipole and dipole-csa interactions as well as the effects of scalar coupling responsible for mixing of magnetization modes during the course o ...	2001	11457242
20 a cd4in16s35(14-) supertetrahedral t4 clusters as building units in decorated cristobalite frameworks.		2001	11457310
historical overview: searching for replication help in all of the rec places.	for several decades, research into the mechanisms of genetic recombination proceeded without a complete understanding of its cellular function or its place in dna metabolism. many lines of research recently have coalesced to reveal a thorough integration of most aspects of dna metabolism, including recombination. in bacteria, the primary function of homologous genetic recombination is the repair of stalled or collapsed replication forks. recombinational dna repair of replication forks is a surpr ...	2001	11459950
the tight linkage between dna replication and double-strand break repair in bacteriophage t4.	double-strand break (dsb) repair and dna replication are tightly linked in the life cycle of bacteriophage t4. indeed, the major mode of phage dna replication depends on recombination proteins and can be stimulated by dsbs. dsb-stimulated dna replication is dramatically demonstrated when t4 infects cells carrying two plasmids that share homology. a dsb on one plasmid triggered extensive replication of the second plasmid, providing a useful model for t4 recombination-dependent replication (rdr). ...	2001	11459966
mediator proteins orchestrate enzyme-ssdna assembly during t4 recombination-dependent dna replication and repair.	studies of recombination-dependent replication (rdr) in the t4 system have revealed the critical roles played by mediator proteins in the timely and productive loading of specific enzymes onto single-stranded dna (ssdna) during phage rdr processes. the t4 recombination mediator protein, uvsy, is necessary for the proper assembly of the t4 presynaptic filament (uvsx recombinase cooperatively bound to ssdna), leading to the recombination-primed initiation of leading strand dna synthesis. in the la ...	2001	11459967
two recombination-dependent dna replication pathways of bacteriophage t4, and their roles in mutagenesis and horizontal gene transfer.	two major pathways of recombination-dependent dna replication, "join-copy" and "join-cut-copy," can be distinguished in phage t4: join-copy requires only early and middle genes, but two late proteins, endonuclease vii and terminase, are uniquely important in the join-cut-copy pathway. in wild-type t4, timing of these pathways is integrated with the developmental program and related to transcription and packaging of dna. in primase mutants, which are defective in origin-dependent lagging-strand d ...	2001	11459968
bacteriophage t4 gene 41 helicase and gene 59 helicase-loading protein: a versatile couple with roles in replication and recombination.	bacteriophage t4 uses two modes of replication initiation: origin-dependent replication early in infection and recombination-dependent replication at later times. the same relatively simple complex of t4 replication proteins is responsible for both modes of dna synthesis. thus the mechanism for loading the t4 41 helicase must be versatile enough to allow it to be loaded on r loops created by transcription at several origins, on d loops created by recombination, and on stalled replication forks. ...	2001	11459969
creating a dynamic picture of the sliding clamp during t4 dna polymerase holoenzyme assembly by using fluorescence resonance energy transfer.	the coordinated assembly of the dna polymerase (gp43), the sliding clamp (gp45), and the clamp loader (gp44/62) to form the bacteriophage t4 dna polymerase holoenzyme is a multistep process. a partially opened toroid-shaped gp45 is loaded around dna by gp44/62 in an atp-dependent manner. gp43 binds to this complex to generate the holoenzyme in which gp45 acts to topologically link gp43 to dna, effectively increasing the processivity of dna replication. stopped-flow fluorescence resonance energy ...	2001	11459977
assembly of reca-like recombinases: distinct roles for mediator proteins in mitosis and meiosis.	members of the reca family of recombinases from bacteriophage t4, escherichia coli, yeast, and higher eukaryotes function in recombination as higher-order oligomers assembled on tracts of single-strand dna (ssdna). biochemical studies have shown that assembly of recombinase involves accessory factors. these studies have identified a class of proteins, called recombination mediator proteins, that act by promoting assembly of recombinase on ssdna tracts that are bound by ssdna-binding protein (ssb ...	2001	11459983
dna replication meets genetic exchange: chromosomal damage and its repair by homologous recombination.	proceedings of the national academy of sciences colloquium on the roles of homologous recombination in dna replication are summarized. current findings in experimental systems ranging from bacteriophages to mammalian cell lines substantiate the idea that homologous recombination is a system supporting dna replication when either the template dna is damaged or the replication machinery malfunctions. there are several lines of supporting evidence: (i) dna replication aggravates preexisting dna dam ...	2001	11459990
prognostic studies on gastric cancer with concomitant liver metastases.	the aim of this study was to clarify prognostic factors after surgical treatments in gastric cancer patients having synchronous liver metastases.	2001	11462950
antioxidants in the treatment of graves disease.	an antioxidant mixture (larotabe) was evaluated in the treatment of graves disease. fifty-six hyperthyroid patients were treated with methimazol (mmi) (a), larotabe (b), or mmi plus larotabe (c). according to a clinical score, improvement was obtained at 8 weeks in a and 4 weeks in b and c. group a diminished their thyroid hormone concentration to normal levels, while patients with larotabe did not reduce t3 and t4 unless mmi was introduced. hyperthyroid patients had increased malondialdehyde (m ...	2001	11463161
prognostic significance of pleural lavage cytology after resection for non-small cell lung cancer.	in the staging of lung cancer, pleural effusion that is malignant on cytologic examination is regarded as t4 disease, and curative resection cannot be performed. we conducted this study to determine whether cancer cells can be present in the pleural cavity with no pleural effusion, to investigate the factors contributing to that occurrence, and to evaluate its prognostic significance.	2001	11463552
is lung cancer surgery justified in patients with direct mediastinal invasion?	to assess the results of the surgical treatment of patients with stage iiib non-small cell lung carcinoma (nsclc) invading the mediastinum (t4).	2001	11463554
risk analysis and long-term survival in patients undergoing resection of t4 lung cancer.	the aim of this study is to identify the risk group of patients with t4 lung cancer who could more likely benefit from surgical resection.	2001	11463555
postoperative complications in relation with induction therapy for lung cancer.	the purpose of this study was to evaluate the risk of lung cancer surgery following induction chemotherapy and/or radiotherapy.	2001	11463562
staging laparoscopy for gastric cancer.	the growing role of multimodal treatment plans for advanced gastric cancer has contributed to the development of more accurate preoperative staging strategies. the high diagnostic efficacy of video-laparoscopy as regards the m factor has been reported by many; preoperative laparoscopy therefore permits to avoid unhelpful surgical exploration in case of peritoneal dissemination of tumor or liver metastases undetected by conventional staging. at memorial sloan kattering cancer center preoperative ...	2001	11464493
increased yield of pcr products by addition of t4 gene 32 protein to the smart pcr cdna synthesis system.	under certain conditions, t4 gene 32 protein is known to increase the efficiency of different enzymes, such as taq dna polymerase, reverse transcriptase, and telomerase. in this study, we compared the efficiency of the smart pcr cdna synthesis kit with and without the t4 gene 32 protein. the use of this cdna synthesis procedure, in combination with t4 gene 32 protein, increases the yield of rt-pcr products from approximately 90% to 150%. this effect is even observed for long mrna templates and l ...	2001	11464524
the effect of low temperature and fasting during the winter on metabolic stores and endocrine physiology (insulin, insulin-like growth factor-i, and thyroxine) of coho salmon, oncorhynchus kisutch.	the objective of this study was to examine the effect of winter feeding and fasting at both high (10 degrees ) and low (2.5 degrees ) temperatures on growth, metabolic stores, and endocrinology of coho salmon. treatments were as follows: warm-fed, warm-not fed, cold-fed, and cold-not fed during the winter (january-february). the following parameters were measured: length, weight, whole body lipid, liver glycogen, hepatosomatic index, and plasma levels of insulin, insulin-like growth factor-i (ig ...	2001	11589631