Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| valproic acid increases histone acetylation and alters gene expression in the donor cells but does not improve the in vitro developmental competence of buffalo (bubalus bubalis) embryos produced by hand-made cloning. | use of histone deacetylase inhibitors (hdacis) is believed to improve the developmental competence and quality of cloned embryos produced. we examined the effects of treatment of buffalo fibroblasts with valproic acid (vpa), a hdaci on these cells and on embryos produced from them by hand-made cloning. vpa treatment (1.5, 3.0, or 4.5 mm) altered (p < 0.05) the growth characteristics and relative expression level of hdac1, dnmt1, dnmt3a, p53, and caspase3, and the global level of h3k9/14ac, h4k5a ... | 2017 | 28055238 |
| chicken egg yolk plasma in tris-citric acid extender improves the quality and fertility of cryopreserved water buffalo (bubalus bubalis) spermatozoa. | this study was primarily designed to evaluate the effect of different concentrations of ultraviolet (uv)-c-irradiated chicken egg yolk plasma (eyp; v:v; 10%, p1; 15%, p2; 20%, p3) or 20% (v:v) of whole chicken egg yolk (wcey) in tris-citric acid (tca) extender on water buffalo sperm quality during cryopreservation (postdilution, pd; postequilibration, pe; post-thawing, pt). also the effect of best evolved concentration of uv-c-irradiated eyp in extender on in vivo fertility of buffalo spermatozo ... | 2017 | 28043368 |
| using real-time pcr to identify pregnancy-associated glycoprotein 2 (pag-2) in water buffalo (bubalus bubalis) blood in early pregnancy. | this study investigates for the first time mrna pregnancy-associated glycoprotein 2 (pag-2) expression in blood cells during early pregnancy in water buffalo. the pags constitute a large family of glycoproteins expressed in the outer epithelial layer of the placenta in eutherian species. all pags are not concomitantly expressed throughout pregnancy; some of them are expressed in the earlier phases, whereas others appear later and are expressed over a shorter period. twenty-one lactating buffaloe ... | 2017 | 28043340 |
| use of post-thaw semen quality parameters to predict fertility of water buffalo (bubalus bubalis) bull during peak breeding season. | this study was designed to predict the fertility of water buffalo bull using post-thaw semen quality parameters during peak breeding season. thirty ejaculates were collected from five bulls with artificial vagina and cryopreserved. at post-thaw, semen was analysed for motility parameters, velocity distribution, kinematics, dna integrity/fragmentation, viability, mitochondrial transmembrane potential, morphology, plasma membrane and acrosome integrity. data of 514 inseminations were collected for ... | 2017 | 27401143 |
| genetic characterization of ovine herpesvirus 2 strains involved in water buffaloes malignant catarrhal fever outbreaks in southern italy. | ovine herpesvirus 2 (ovhv-2) was responsible for two outbreaks of malignant catarrhal fever (mcf) on two water buffalo farms in southern italy. in this study, the presence of this virus in the nasal swabs from sick animals as well as in the organs of dead buffaloes was ascertained by a real-time pcr assay. positive samples also underwent a relative quantitative analysis of the viral dna in them. all the dead animals had the highest relative viral quantities, while buffaloes recovering from the v ... | 2017 | 28110782 |
| pharmacokinetics of amoxicillin trihydrate in thai swamp buffaloes (bubalus bubalis): a pilot study. | this study aimed to investigate the pharmacokinetic characteristics of amoxicillin (amx) in thai swamp buffaloes, bubalus bubalis, following single intramuscular administration at two dosages of 10 and 20 mg/kg body weight (b.w.). blood samples were collected at assigned times up to 48 h. the plasma concentrations of amx were measured by liquid chromatography-tandem mass spectrometry (lc-ms/ms). the concentrations of amx in the plasma were determined up to 24 h after i.m. administration at both ... | 2017 | 27292774 |
| transcriptomic responses of water buffalo liver to infection with the digenetic fluke fasciola gigantica. | fasciola gigantica, the tropical liver fluke, infects buffaloes in asian and african countries and causes significant economic losses and poses public health threat in these countries. however, little is known of the transcriptional response of buffaloes to infection with f. gigantica. the objective of the present study was to perform the first transcriptomic analysis of buffalo liver infected by f. gigantica. understanding the mechanisms that underpin f. gigantica infection in buffaloes will co ... | 2017 | 28143561 |
| development of an indirect elisa using different fragments of recombinant ncgra7 for detection of neospora caninum infection in cattle and water buffalo. | dense granules are immunodominant proteins for the standardization of immunodiagnostic procedures to detect neosporosis. in the presented study different fragment of a dense-granule protein was evaluated for serodiagnosis of neospora caninum in cattle and water buffalo. | 2017 | 25904948 |
| investigation of the prion protein gene (prnp) polymorphisms in anatolian, murrah, and crossbred water buffaloes (bubalus bubalis). | bovine spongiform encephalopathy (bse) of the cattle is the outstanding disease among other transmissible spongiform encephalopathy (tses). it can be transmitted from the cattle to a human and causes a new variant of the creutzfeldt-jakob disease (cjd). it is known that prion protein coding gene (prnp) plays a major role in the tse susceptibility or resistance in some species. recent researches demonstrated that the insertion (in) and deletion (del) polymorphisms within promoter and intron 1 reg ... | 2017 | 27822596 |
| detection of pseudocowpox virus in water buffalo (bubalus bubalis) with vesicular disease in the state of são paulo, brazil, in 2016. | parapoxviruses are zoonotic viruses that infect cattle, goats and sheep; there have also been reports of infections in camels, domestic cats and seals. | 2017 | 27774853 |
| origins and global context of brucella abortus in italy. | brucellosis is a common and chronic disease of cattle and other bovids that often causes reproductive disorders. natural infection in cattle is caused by brucella abortus and transmission typically occurs during abortions, calving, or nursing. brucellosis is also a major zoonotic disease due to contamination of dairy products or contact with the tissues of infected animals. brucellosis has been eradicated from most of the developed world in the last 40 years but persists in many regions-the dise ... | 2017 | 28152976 |
| a serosurvey for ruminant pestivirus exposure conducted using cattle sera collected for brucellosis surveillance in the united states. | four species of ruminant pestivirus are currently circulating in the united states: bovine viral diarrhea virus 1 and 2 (bvdv-1, -2; predominant host: cattle), border disease virus (bdv; predominant host: sheep), and pronghorn virus (sporadically detected in wild ruminants). a third bovine pestivirus called hobi-like virus has been detected in cattle and water buffalo in south america, asia, and europe. to date, no isolations of hobi-like viruses from u.s. cattle have been reported. to assess ex ... | 2017 | 28074709 |
| genome sequence of bubaline alphaherpesvirus 1 (buhv1) isolated in australia in 1972. | bubaline alphaherpesvirus 1 (buhv1) is a member of the family herpesviridae, subfamily alphaherpesvirinae, genus varicellovirus. to date, no full genome sequence of buhv has been published. here, we report the complete genome sequence of bubaline alphaherpesvirus 1 (buhv1) strain b6 (buhv1-b6), isolated from a water buffalo (bubalus bubalis) in 1972 in australia. the virus was multiplied in mdbk cells, and the dna was extracted and subjected to high-throughput sequencing. the reads were aligned ... | 2017 | 28063080 |
| characterization of a recombinant newcastle disease virus expressing the glycoprotein of bovine ephemeral fever virus. | bovine ephemeral fever (bef) is caused by the arthropod-borne bovine ephemeral fever virus (befv), which is a member of the family rhabdoviridae and the genus ephemerovirus. befv causes an acute febrile infection in cattle and water buffalo. in this study, a recombinant newcastle disease virus (ndv) expressing the glycoprotein (g) of befv (rl-befv-g) was constructed, and its biological characteristics in vitro and in vivo, pathogenicity, and immune response in mice and cattle were evaluated. bef ... | 2017 | 27757685 |
| the apicoplast genomes of two taxonomic units of babesia from sheep. | the apicoplast (ap) is a unique, non-photosynthetic organelle found in most apicomplexan parasites. due to the essential roles that this organelle has, it has been widely considered as target for drugs against diseases caused by apicomplexans. exploring the ap genomes of such parasites would provide a better understanding of their systematics and their basic molecular biology for therapeutics. however, there is limited information available on the ap genomes of apicomplexan parasites. in the pre ... | 2017 | 27916258 |
| elucidating the genetic basis of an oligogenic birth defect using whole genome sequence data in a non-model organism, bubalus bubalis. | recent strong selection for dairy traits in water buffalo has been associated with higher levels of inbreeding, leading to an increase in the prevalence of genetic diseases such as transverse hemimelia (th), a congenital developmental abnormality characterized by absence of a variable distal portion of the hindlimbs. limited genomic resources available for water buffalo required an original approach to identify genetic variants associated with the disease. the genomes of 4 bilateral and 7 unilat ... | 2017 | 28045068 |
| chemical characterization of the milk oligosaccharides of some artiodactyla species including giraffe (giraffa camelopardalis), sitatunga (tragelaphus spekii), deer (cervus nippon yesoensis) and water buffalo (bubalus bubalis). | mammalian milk/colostrum usually contains oligosaccharides along with the predominant disaccharide lactose. it has been found that the number and identity of these milk oligosaccharides varies among mammalian species. oligosaccharides predominate over lactose in the milk/colostrum of arctoidea species (carnivora), whereas lactose predominates over milk oligosaccharides in artiodactyla including cow, sheep, goat, camel, reindeer and pig. to clarify whether heterogeneity of a variety of milk oligo ... | 2018 | 30467790 |
| novel extraction of high quality genomic dna from frozen bovine blood samples by using detergent method. | dna is the prerequisite for life's inception that transfers hereditary information, past several years; various types of commercial kits are made available which vary depending on the type of the biological sample being used. the present study is focused on developing an improvised methodology for the isolation of genomic dna from stored bovine blood samples. dna was isolated by using the conventional phenol: chloroform: isoamyl alcohol (pci) method and detergent method. the aim of the study was ... | 2018 | 30538933 |
| treatment with chemical delipidation forskolin prior to cryopreservation improves the survival rates of swamp buffalo (bubalus bubalis) and bovine (bos indicus) in vitro produced embryos. | the cryopreservation of embryos is a technology developed for long-term genetic preservation. however, high sensitivity to low temperatures due to a large number of intracellular lipids within ruminant embryos compromises the success of this technique. the aim of this study was to examine the effects of using of lipolytic chemical agent forskolin, during in vitro producing of buffalo and bovine embryos on lipid contents, cryotolerance and subsequent developmental competence of these embryos. buf ... | 2018 | 30092172 |
| molecular surveillance and phylogenetic traits of babesia bigemina and babesia bovis in cattle (bos taurus) and water buffaloes (bubalus bubalis) from colombia. | babesia bigemina and b. bovis are two economically important hemoparasites affecting both cattle and buffaloes involved in dairy and beef production. in colombia, although some parasitological and serological studies suggest an endemicity of these pathogens in areas under 1000 m, little is known about its molecular prevalence in different host. the objective of this study was to estimate the prevalence and molecular traits of these parasites in cattle and buffaloes from two colombian regions. | 2018 | 30208941 |
| genomic structural differences between cattle and river buffalo identified through comparative genomic and transcriptomic analysis. | water buffalo (bubalus bubalis l.) is an important livestock species worldwide. like many other livestock species, water buffalo lacks high quality and continuous reference genome assembly, required for fine-scale comparative genomics studies. in this work, we present a dataset, which characterizes genomic differences between water buffalo genome and the extensively studied cattle (bos taurus taurus) reference genome. this data set is obtained after alignment of 14 river buffalo whole genome seq ... | 2018 | 29892639 |
| a review of coccidiosis in water buffaloes (bubalus bubalis). | the water buffalo (bubalus bubalis) is important to the economy of several countries in asia, and south america and there are also isolated herds in europe. in india, buffalo is the main dairy animal. coccidiosis due to eimeria is an important cause of diarrhea in livestock worldwide. eimeria species are generally considered host specific. more than 11 eimeria species are considered common in buffaloes and cattle (bos taurus, bos indicus). of the numerous species of eimeria in cattle, eimeria bo ... | 2018 | 29887030 |
| mitigated clinical disease in water buffaloes experimentally infected with babesia bovis. | water buffaloes (bubalus bubalis) are raised in tropical and subtropical regions of the world, and act as hosts of babesia bovis parasites and the tick vector rhipicephalus microplus. as no clinical cases of b. bovis-infection have been reported, we hypothesized that, unlike bovines, water buffaloes respond asymptomatically to an acute infection. to test this hypothesis, we inoculated two groups of 24-month-old mediterranean breed water buffaloes with 108 erythrocytes infected with two argentine ... | 2018 | 29724619 |
| buffalo species identification and delineation using genetic barcoding markers. | enrichment of barcode databases with mitochondrial cytochrome c oxidase subunit i (coi) barcode sequences in different animal taxa has become important for identification of animal source in food samples to prevent commercial fraud. in this study, coi barcode sequence in seventy one river buffalo samples were determined, analyzed and deposited in genbank barcode database and barcode of life database (bold) to contribute for construction of public reference library for coi barcode sequence in riv ... | 2018 | 30733766 |
| normal magnetic resonance anatomy of the hind foot of egyptian buffalo (bubalus bubalis): a correlative low-field t1- and t2-weighted mri and sectional anatomy atlas. | buffaloes represent a major source of milk production, especially in developing countries including egypt. the buffalo foot is frequently involved in a large proportion of lameness cases. the relatively small size and complexity of its structures often render the radiographic evaluation of the foot challenging. magnetic resonance imaging (mri) is a noninvasive imaging technique that is regarded as both safe and accurate for assessment of the foot disorders in both man and animals. the purpose of ... | 2018 | 30221389 |
| expression profile of the csf3 and lpo genes in milk from buffalo (bubalus bubalis) with and without mastitis. | this study compared the expression profile of the candidate genes, csf3 and lpo, by investigating the immune response mechanisms involved in the phenotype of resistance and susceptibility to mastitis of healthy and infected buffaloes. the granulocyte colony stimulating factor 3 (csf3) and lactoperoxidase (lpo) genes expression profiles were determined in 24 milk samples from buffaloes with (n = 12) and without (n = 12) mastitis, using the quantitative real-time pcr (qrt-pcr) technique. csf3 and ... | 2018 | 30217657 |
| reactive oxygen metabolites in alpha-herpesvirus-seropositive mediterranean buffaloes (bubalus bubalis): a preliminary study. | in the present study on bubalus bubalis of the campania region (italy) the serum levels of derivatives of reactive oxygen metabolites (d-roms), anti-rom and oxidative stress index (osi) were evaluated. these data were then related to the seropositive status of the animals against alpha-herpesviruses, precisely bubaline herpesvirus 1 (buhv-1) and bovine herpesvirus 1 (bohv-1). clinically healthy mediterranean buffaloes were selected for this study. the serum samples of these animals were taken, a ... | 2018 | 30468352 |
| established method of chondroitin sulphate extraction from buffalo (bubalus bubalis) cartilages and its identification by ftir. | a study was conducted for extraction of chondroitin sulphate (cs) from buffalo tracheal, nasal and joint cartilages. cs was extracted from cartilages using 0.25% papain digestion, dialyzed, precipitated with 10% tca and finally lyophilized to dry powder. dimethylmethylene blue assay was performed to estimate the quantity of cs extracted. identification of extracted cs was performed with sds-page and fourier transforms infrared spectroscopy (ftir). sds-page analysis of extracted cs revealed simil ... | 2018 | 30150802 |
| recombinant interferon stimulated protein 15 (risg15) as a molecular marker for detection of early pregnancy in bubalus bubalis. | early and accurate diagnosis of pregnancy in animals is important for improving the reproductive management of livestock. the buffalo (bubalus bubalis) is the most important dairy animal in india, but there are reproductive problems resulting from extended calving interval and ovulation occurring in the absence of behavioral estrus. the lack of simple methods for early pregnancy diagnosis intensifies these problems. the present study, therefore, was conducted to ascertain the role of the interfe ... | 2018 | 30145042 |
| presence of cadmium residues in muscle, liver and kidney of bubalus bubalis and histological evidence. | cadmium (cd) concentrations were evaluated in the samples of kidney, liver and muscle of sixty-six buffaloes regularly slaughtered. forty were raised in campania, in the territory between the province of naples and caserta and twenty-six were bred in apulia, in the province of bari. two aliquots were prepared for the renal and hepatic samples: one intended for the chemical analysis and the other one intended for histological investigations. muscle samples were the subject of purely chemical inve ... | 2018 | 30538963 |
| scrotal infrared digital thermography predicts effects of thermal stress on buffalo (bubalus bubalis) semen. | the objective was to use scrotal infrared digital thermography to evaluate effects of thermal stress on semen quality of murrah buffalo (bubalus bubalis) breeding bulls. ejaculates from 109 murrah bulls maintained at three semen stations were evaluated for: ejaculate volume, sperm concentration (scon), initial motility (im), percent live sperm (live), acrosome integrity of fresh semen (aifs), plasma membrane integrity of fresh semen (pmifs), head abnormalities of fresh semen (hafs), midpiece abn ... | 2018 | 30509667 |
| occurrence of amblyomma mixtum on the water buffalo (bubalus bubalis) in mexico. | the water buffalo (bubalus bubalis) is an asian species of bovine that was introduced in mexico in 1992 as an alternative for milk and meat production. to date, no surveys have been conducted to identify ticks acquired by water buffaloes since their arrival in the country. here we report, for the first time, the presence of amblyomma mixtum, a neotropical tick in the amblyomma cajennense complex, found on water buffaloes from mexico and discuss its possible implications in veterinary public heal ... | 2018 | 30386724 |
| polymorphism of melatonin receptor (mtnr1a) gene and its association with seasonal reproduction in water buffalo (bubalus bubalis). | the water buffalo have a seasonal pattern of reproduction with decreased sexual activity during the longer photoperiod. the present study was designed to identify a single nucleotide polymorphism (snp) in the mtnr1 a gene and its association with seasonal reproduction and reproductive characteristics in murrah buffalo cows. the 812 bp fragment encompassing exon ii of the mtnr1 a gene was amplified from genomic dna of 190 pluriparous murrah buffalo cows. amplified pcr products from 12 samples wer ... | 2018 | 30384994 |
| propensity in low-grade oocytes for delayed germinal vesicle breakdown compromises the developmental ability of sub-optimal grade bubalus bubalis oocytes. | summarymaturing oocytes have diverse developmental potential and good quality oocytes exhibit a better ability to attain physiological milestones in a time-dependent manner. this situation necessitates the confirmation of oocyte developmental status more precisely under an in vitro embryo production (ivep) regime. the aim of this study was to explain timely events in germinal vesicle breakdown (gvbd), an important milestone of oocyte nuclear maturation, to delineate the developmental capacity of ... | 2018 | 30289096 |
| selenium and copper interaction at supra-nutritional level affecting blood parameters including immune response against p. multocida antigen in murrah buffalo (bubalus bubalis) calves. | minerals play important role in the diet of an animal. bio-availability of minerals largely gets affected by absolute as well as the relative amount of each mineral present in the diet of an animal. copper and selenium are two such an essential elements affect utilization of each other in the gastrointestinal tract. the present study elucidates the utilization of copper and selenium at supra-nutritional levels (higher than nutritional requirements). male murrah buffalo (bubalus bubalis) calves ( ... | 2018 | 30262314 |
| evaluation of pigeon egg yolk for post thaw quality, enzyme leakage and fertility of buffalo (bubalus bubalis) bull spermatozoa. | egg yolk is used as a cryoprotectant in semen preservation. however, its composition varies according to the species which may influence its effectiveness during the freeze-thaw process. therefore, study was conducted to identify the optimum level of pigeon egg yolk (pey) in tris citric acid (tca) extender for freezability and in vivo fertility of buffalo semen. semen was collected at weekly intervals for a period of three weeks (replicates) from 6 nili ravi buffalo bulls (2 ejaculates/bull/repl ... | 2018 | 30006129 |
| the snp g.4667g>a at 3'-utr of ifng gene is associated with susceptibility to bovine tuberculosis in mediterranean water buffalo (bubalus bubalis). | 2018 | 29999184 | |
| molecular identification of buxtonella sulcata from associated-diarrhea in water buffaloes (bubalus bubalis) in the philippines | sixty suspected protozoan oocysts were demonstrated from 260 fecal samples collected from water buffaloes aged one month to seven years old with clinical signs of diarrhea in four provinces in the philippines after conventional methods of isolation, sporulation, morphological characteristics and kinyoun acid fast staining techniques. the recovered protozoan oocysts were subjected to molecular analysis. amplification of dna extractedfrom recovered eimeria oocysts using universal primers for the i ... | 2018 | 29983020 |
| cytoplasmic volume of recipient oocytes affects the nucleus reprogramming and the developmental competence of hmc buffalo (bubalus bubalis) embryos. | the present study was undertaken to examine the effects of cytoplasmic volume on nucleus reprogramming and developmental competence of buffalo handmade cloning (hmc) embryos. we found that both hmc embryos derived from ~150% cytoplasm or ~225% cytoplasm resulted in a higher blastocyst rate and total cell number of blastocyst in comparison with those from ~75% cytoplasm (25.4 ± 2.0, 27.9 ± 1.6% vs. 17.9 ± 3.1%; 150 ± 10, 169 ± 12 vs. 85 ± 6, p<0.05). meanwhile, the proportions of nuclear envelope ... | 2018 | 29925699 |
| comparison of tris egg yolk-based, triladyl® and optixell® extender on post-thaw quality, kinematics and in vivo fertility of nili ravi buffalo (bubalus bubalis) bull spermatozoa. | our objectives were to ascertain the comparison of tris egg yolk-based, triladyl® and optixell® extender on postthaw quality, casa parameters and in vivo fertility of nili ravi buffalo (bubalus bubalis) bull spermatozoa. semen samples (n = 35) from five bulls were diluted in tris egg yolk-based, triladyl® , optixell® extender and frozen in 0.50 ml french straws. postthaw sperm casa motility (%) was higher (p < 0.05) in optixell extender as compared to triladyl and tris egg yolk-based extender. a ... | 2018 | 29920732 |
| effects of an automatic milking system on milk yield and quality of mediterranean buffaloes. | a study was carried out to evaluate the effects of an automatic milking system (ams) on milk yield and composition of buffalo (mediterranean-type bubalus bubalis) cows. performed from january 2015 to december 2015 in an organic buffalo dairy farm equipped with both a traditional tandem milking parlor and an ams, the study involved 90 primiparous buffaloes randomly allotted to a tandem or ams group from 5 to 10 d of lactation onward. number of milkings per day and daily milk yield of each cow wer ... | 2018 | 29908804 |
| label-free proteome of water buffalo (bubalus bubalis) seminal plasma. | the study aimed to describe the bubalus bubalis seminal plasma proteome using a label-free shotgun udmse approach. a total of 859 nonredundant proteins were identified across five biological replicates with stringent identification. proteins specifically related to sperm maturation and protection, capacitation, fertilization and metabolic activity were detected in the buffalo seminal fluid. in conclusion, we provide a comprehensive proteomic profile of buffalo seminal plasma, which establishes a ... | 2018 | 29888513 |
| prevalence and risk factors associated with ectoparasite infestation of buffaloes in an amazonian ecosystem. | the water buffalo (bubalus bubalis) is well adapted in some regions of the amazon. of all brazilian states, pará contains the largest number of this species, with 510,000 animals, approximately 38% of the brazilian buffaloes. despite the socioeconomic importance of bubaline farming in the northern region, little is known about the prevalence of ectoparasites that affect buffalo herds. this study aimed to identify the species of buffalo ectoparasites in the municipality of santarém, pará, and to ... | 2018 | 29866180 |
| investigation of the persistence of penicillin g and dihydrostreptomycin residues in milk of lactating buffaloes ( bubalus bubalis) using ultra-high-performance liquid chromatography and tandem mass spectrometry. | the purpose of this research was to evaluate the persistence of penicillin g and dihydrostreptomycin in milk of lactating buffaloes following intramuscular injection of procaine penicillin g (200000 iu/ml) and dihydrostreptomycin sulfate (250 mg/ml) every 24 h for 3 days. milk samples were collected twice daily up to the 13th milking post-treatment and analyzed by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry. the analytical method has been validated according ... | 2018 | 29862808 |
| comparative diversity analysis of ruminal methanogens in murrah buffaloes (bubalus bubalis) in four states of north india. | we compared the community structure of methanogens in murrah breed of buffaloes of four states of north india using 16s rrna gene clone library method. the results revealed the dominance of methanogens related to methanobrevibacter in three states, while methanomicrobium-related methanogens were abundant in one state. | 2018 | 29859302 |
| bovine viral diarrhea virus subgenotype 1b in water buffalos (bubalus bubalis) from brazil. | serological studies have characterized the presence of the bovine viral diarrhea virus (bvdv) infection in water buffalo herds worldwide. however, the molecular characterization of bvdv strains circulating in this animal species is uncommon. the aim of this study was to identify young water buffalo with acute infection and characterize the subgenotype of the infecting wild-type bvdv strain. two dairy water buffalo herds from northeastern brazil were selected based on the results of virus neutral ... | 2018 | 29855763 |
| developmental and molecular responses of buffalo (bubalus bubalis) cumulus-oocyte complex matured in vitro under heat shock conditions. | summaryto investigate the effects of physiologically relevant heat shock during oocyte maturation, buffalo cumulus-oocyte complexes (cocs) were cultured at 38.5°c (control) or were exposed to 39.5°c (t1) or 40.5°c (t2) for the first 6 h of in vitro maturation (ivm), followed by 38.5°c through the next 18 h/ivm and early embryonic development up to the blastocyst stage. gene expression analysis was performed on selected target genes (hsf-1, hsf-2, hsp-70, hsp-90, bax, p53, sod1, cox1, mapk14) in ... | 2018 | 29785894 |
| reference 1d and 2d electrophoresis maps for potential disease related proteins in milk whey from lactating buffaloes and blood serum from buffalo calves (water buffalo, bubalus bubalis). | the aim of this study was to identify potential disease related proteins in milk whey of lactating buffaloes and blood serum of buffalo calves, in order to define a reference electrophoresis map for 1-de and 2-de. additionally, changes in some protein patterns from buffalo calves during salmonellosis and lactating buffaloes during mastitis are presented. milk samples were collected and distributed into groups: milk samples from healthy buffaloes (scc < 100.000 cells/ml, negative microbiology and ... | 2018 | 29734122 |
| m-carboxycinnamic acid bishydroxamide improves developmental competence, reduces apoptosis and alters epigenetic status and gene expression pattern in cloned buffalo (bubalus bubalis) embryos. | incomplete or aberrant reprogramming of nuclear genome is one of the major problems in somatic cell nuclear transfer. in this study, we studied the effect of histone deacetylase inhibitor m-carboxycinnamic acid bishydroxamide (cbha) on in vitro development of buffalo embryos produced by hand-made cloning. cloned embryos were treated with cbha (0, 5, 10, 20 or 50 μm) for 10 hr from the start of reconstruction till activation. at 10 μm, but not at other concentrations examined, cbha increased (p < ... | 2018 | 29733525 |
| efficient generation of transgenic buffalos (bubalus bubalis) by nuclear transfer of fetal fibroblasts expressing enhanced green fluorescent protein. | the possibility of producing transgenic cloned buffalos by nuclear transfer of fetal fibroblasts expressing enhanced green fluorescent protein (egfp) was explored in this study. when buffalo fetal fibroblasts (bffs) isolated from a male buffalo fetus were transfected with pegfp-n1 (egfp is driven by cmv and neo is driven by sv-40) by means of electroporation, lipofectamine-ltx and x-tremegene, the transfection efficiency of electroporation (35.5%) was higher than lipofectamine-ltx (11.7%) and x- ... | 2018 | 29725050 |
| re-evaluation of endogenous development of eimeria bareillyi gill, chhabra and lall, 1963 in water buffalo (bubalus bubalis). | water buffalo (bubalus bubalis) is important for the economy of asia, south america and parts of europe. coccidiosis is an important cause of neonatal mortality in livestock, including buffalo. of more than 12 species of eimeria in buffalo, eimeria bareillyi is the most pathogenic. there are uncertainties concerning its asexual and sexual development. during a previously reported outbreak of fatal enteritis associated with e. bareillyi in buffaloes in the netherlands, sections of small intestine ... | 2018 | 29692286 |
| domestic water buffaloes: access to surface water, disease prevalence and associated economic losses. | given the shortage and non-availability of freshwater in pakistan, wastewater is being used for bathing water buffaloes; however, this has a negative impact on animal welfare. although there is a vast literature on indirect linkages between wastewater and animal productivity, studies focusing on the direct impacts of water buffaloes bathing in wastewater on animal productivity and economic losses are rare. therefore, using 360 domestic water buffalo farms, this study examines the expenditure and ... | 2018 | 29685433 |
| testosterone levels and the genetic variation of sex hormone-binding globulin gene of bubalus bubalis, bulls in egypt. | testosterone is themain plasma androgen produced and secreted by male testis. to be bioactive, it binds to sex hormonebinding globulin (shbg), i.e. the major transporter protein of sex steroids in the blood of mammals and other vertebrate species.firstly, this study aimed to determine the levels of testosterone in different-age groups of buffalo bulls (bubalus bubalis) in egypt, and secondly to screen the genetic polymorphisms in their shbg gene and to investigatewhether these polymorphisms are ... | 2018 | 29666348 |
| antimicrobial peptides of buffalo and their role in host defenses. | antimicrobial peptides (amps) are highly conserved components of the innate immune system found among all classes of life. buffalo (bubalus bubalis), an important livestock for milk and meat production, is known to have a better resistance to many diseases as compared to cattle. they are found to express many amps such as defensins, cathelicidins, and hepcidin which play an important role in neutralizing the invading pathogens. buffalo amps exhibit broad-spectrum antimicrobial activity against b ... | 2018 | 29657403 |
| first molecular characterization and morphological aspects of sarcocystis fusiformis infecting water buffalo bubalus bubalis in egypt. | fresh muscle samples from water buffalo (bubalus bubalis) aged 2-15, from giza province, egypt; were examined for sarcocystis infection. macroscopic ovoid sarcocysts embedded in the muscle tissues of the examined buffaloes were detected; they measured 152-230 (210 ± 7) μm in length and 37-119 (95 ± 3) μm in width. the esophagus was the most infected organ followed by the diaphragm, and tongue, while the heart muscles were the least infected. the cyst cavity was compartmentalized by septa derived ... | 2018 | 29654684 |
| cloning of buffalo, a highly valued livestock species of south and southeast asia: any achievements? | buffalo (bubalus bubalis) is a major source of milk, meat, and draught power in many developing countries in asia. animal cloning holds a lot of potential for fast multiplication of elite buffaloes and conservation of their valuable germplasm. although the progress of buffalo cloning has been slow in comparison to cattle or pig, several breakthroughs were reported in buffalo cloning such as the production of cloned calves from somatic cells isolated from over one-decade old frozen-thawed semen o ... | 2018 | 29620444 |
| metabolic and hormonal adaptation in bubalus bubalis around calving and early lactation. | pregnancy and lactation are physiological periods that result in an increased metabolic demand that, if not satisfied, could provoke a threat to homeostasis. in this study changes in the values of non-esterified fatty acids (nefa) and β-hydroxybutyrate (bhb), glucose, insulin, thyroid hormones, milk composition and yield were investigated in buffaloes during the late pregnancy and early lactation. from a total of 50 buffaloes, blood samples were collected -7±5 days before expected calving; +7±5; ... | 2018 | 29617370 |
| blood gas and serum biochemical ris for healthy newborn murrah buffaloes (bubalus bubalis). | there is a lack of published work on ris for newborn buffaloes. establishing blood gas and serum biochemical ris for newborn buffaloes is important for monitoring health. | 2018 | 29575143 |
| remarkable genetic diversity detected at river buffalo prolactin receptor (prlr) gene and association studies with milk fatty acid composition. | prolactin is an anterior pituitary peptide hormone involved in many different endocrine activities and is essential for reproductive performance. this action is mediated by its receptor, the prolactin receptor, encoded by the prlr gene. in this study, we sequenced and characterized the mediterranean river buffalo prlr gene (from exon 3 to 10), and we found remarkable genetic diversity. in particular, we found 24 intronic polymorphisms and 13 exonic snps, seven of which were non-synonymous. furth ... | 2018 | 29569734 |
| new insights on water buffalo genomic diversity and post-domestication migration routes from medium density snp chip data. | the domestic water buffalo is native to the asian continent but through historical migrations and recent importations, nowadays has a worldwide distribution. the two types of water buffalo, i.e., river and swamp, display distinct morphological and behavioral traits, different karyotypes and also have different purposes and geographical distributions. river buffaloes from pakistan, iran, turkey, egypt, romania, bulgaria, italy, mozambique, brazil and colombia, and swamp buffaloes from china, thai ... | 2018 | 29552025 |
| a review on breeding and genetic strategies in iranian buffaloes (bubalus bubalis). | the aim of current study was to review breeding progress and update information on genetic strategies in iranian buffaloes. iranian buffalo is one of the vital domestic animals throughout north, north-west, south and south-west of iran with measurable characteristics both in milk and meat production. the species plays an important role in rural economy of the country due to its unique characteristics such as resistance to diseases and parasites, having long productive lifespan and showing higher ... | 2018 | 29524107 |
| isolation and characterization of buffalo (bubalus bubalis) amniotic mesenchymal stem cells derived from amnion from the first trimester pregnancy. | amniotic mesenchymal stem cells (amscs) from livestock are valuable resources for animal reproduction and veterinary therapeutic. the purpose of this study is to explore a suitable way to isolate and culture the buffalo amscs (bamscs), and to identify their biological characteristics. digestion with a combination of trypsin-edta and collagenase type i could obtain pure bamscs more effectively than trypsin-edta or collagenase type i alone. bamscs could proliferate steadily in vitro culture and ex ... | 2018 | 29515060 |
| first identification of cryptosporidium parvum subtype iiaa20g1r1 in water buffalos (bubalus bubalis). | cryptosporidium can infect a wide variety of vertebrate animals, including mammals, birds, amphibians, reptiles, and fish. there are few molecular characterizations of cryptosporidium isolated from water buffalo. thus, the present study investigated the occurrence and molecular characterization of cryptosporidium spp. in water buffalos by nested-pcr. non-diarrheic feces were obtained from 122 water buffalo calves. all samples were tested by nested-pcr based on the 18s rrna gene, after which posi ... | 2018 | 29514125 |
| selection of suitable reference genes for normalization of quantitative rt-pcr (rt-qpcr) expression data across twelve tissues of riverine buffaloes (bubalus bubalis). | selection of reference genes has become an integral step in any real time quantitative pcr (rt-qpcr) based expression studies. the importance of this study stems from the fact that riverine buffaloes are major dairy species of indian sub-continent and the information generated here will be of great interest to the investigators engaged in functional genomic studies of this important livestock species. in this study, an effort was made to evaluate a panel of 10 candidate reference genes (glyceral ... | 2018 | 29509770 |
| molecular evidence of the reservoir competence of water buffalo (bubalus bubalis) for anaplasma marginale in cuba. | water buffalo (bubalus bubalis) is a potential reservoir for anaplasma marginale in livestock ecosystems of tropical countries. however, their participation in the epidemiological process of bovine anaplasmosis in endemic areas remains unclear. in the present study, the reservoir competence of water buffalo for a. marginale was explored by focusing on the analysis of rickettsemia levels in carrier animals, and the genetic characterization of a. marginale strains from cattle and buffalo. eight gr ... | 2018 | 31014871 |
| retinoic acid improves maturation rate and upregulates the expression of antioxidant-related genes in in vitro matured buffalo (bubalus bubalis) oocytes. | retinoic acid, vitamin a metabolite, plays a role in oocyte development and maturation in different ways including gene expression alteration and/or prohibiting oxidative stress. the objective of this study was to examine the effect of 9-cis-retinoic acid (9-cisra) on the quality and maturation rate of buffalo oocytes. cumulus-oocyte complexes (cocs, n = 460) were collected from ovaries of slaughtered buffalos. varying concentrations of 9-cisra (0, 5, 50, and 200 nm) were added to the maturation ... | 2018 | 30564610 |
| cytogenetic identification of khuzestani water buffalo. | the domestic water buffalo (bubalus bubalis) is an economically important livestock species in many asian and mediterranean countries. two types of water buffalos are river and swamp buffaloes. genetically, the swamp buffalo has 48 chromosomes (2n = 48) and the river buffalo has 50 chromosomes (2n = 50). khuzestan province is one of the important centers of buffalo farming in iran. since there was no cytogenetic information about khuzestani buffalo breed, the present study was performed to provi ... | 2018 | 30713615 |
| validation of an inhibition enzyme-linked immunosorbent assay system for the diagnosis of toxoplasma gondii infection in buffaloes (bubalus bubalis). | toxoplasmosis is one of the most widespread zoonoses in the world, due to the existence of a wide variety of toxoplasma gondii hosts, which include several domestic animal species. in cuba, there is sustained production of the bubalus species, which is highly adaptable and disease resistant, although it has been identified as a reservoir for a range of aetiological agents. several countries have reported buffaloes as the intermediate host of t. gondii, noting the need to carry out epidemiologica ... | 2018 | 30964470 |
| characterization of naturally occurring, new and persistent subclinical foot-and-mouth disease virus infection in vaccinated asian buffalo in islamabad capital territory, pakistan. | the convalescent subclinical carrier state of foot-and-mouth disease virus (fmdv) infection has been thoroughly investigated; contrastingly, the subclinical form of new infections of vaccinated and naïve hosts is recognized, but poorly understood. to investigate the natural dynamics of subclinical fmdv infections, a prospective, 12-month, longitudinal study was conducted in vaccinated asian buffalo (bubalus bubalis) under natural conditions in pakistan, where fmdv is hyperendemic. oropharyngeal ... | 2018 | 30035376 |
| an update: reproductive handmade cloning of water buffalo (bubalus bubalis). | the first birth of a cloned animal produced through the handmade cloning (hmc) technique was reported more than 15 years ago in cattle. this method of somatic cell nuclear transfer (scnt) has subsequently been evolving as a much simpler alternative to the classical micromanipulator-based scnt. several farm animal species such as cattle, buffalo, pigs, sheep, and goats have been successfully cloned using hmc. in buffalo, hmc technique is now well established, and several births of cloned calves h ... | 2018 | 30122268 |
| developmental competence of buffalo (bubalus bubalis) denuded oocytes cocultured with cumulus cells: protective role of cumulus cells. | the objectives of the present study were to evaluate the developmental competence of buffalo denuded oocytes (dos) cocultured with cumulus cells (ccs) during in vitro maturation, and to investigate the mechanisms by which ccs promote oocyte maturation and development. buffalo oocytes were matured in vitro for 24 h in three groups: (1) intact cumulus-oocyte complexes (cocs) (2) dos cocultured with ccs (doscc), and (3) dos cultured alone (dos). matured oocytes were used to determine the relative m ... | 2018 | 30092373 |
| epidural anesthesia in egyptian water buffalo (bubalus bubalis): a comparison of lidocaine, xylazine and a combination of lidocaine and xylazine. | to evaluate and compare the analgesic effects of a combination of lidocaine and xylazine to lidocaine or xylazine administered alone for epidural anesthesia in egyptian water buffalo (bubalus bubalis). | 2018 | 30078532 |
| effect of consecutive re-synchronization protocols on pregnancy rate in buffalo (bubalus bubalis) heifers out of the breeding season. | the combined effect of six consecutive timed artificial inseminations (tais) on pregnancy rates, following two different synchronization protocols on buffalo heifers, over a period of seven months typically characterized by low breeding performances, were investigated in this study. a total of 2189 tais were performed on 1463 buffalo heifers within a large buffalo farm in the south of italy. individual animals were allowed to undergo synchronization protocol (either a slightly modified ovsynch o ... | 2018 | 29494837 |
| hepatobiliary diseases in buffalo (bubalus bubalis): clinical, laboratory, and ultrasonographic findings. | this study describes ultrasonographic observations of five hepatobiliary diseases in buffalo (bubalus bubalis). fifty buffalo, including 20 clinically normal and 30 hepatobiliary diseased buffalo were enrolled in the study. complete clinical, radiographic and ultrasonographic examinations and laboratory analyses were conducted. focal parenchymal lesions including liver abscess (n = 12) and hepatic cyst (n = 6), diffuse parenchymal lesion (hepatobiliary cirrhosis, n = 5) and obstruction of hepato ... | 2018 | 29486538 |
| genome-wide gene expression analysis of 45 days pregnant fetal cotyledons vis-a-vis non-pregnant caruncles in buffalo (bubalus bubalis). | the crosstalk between fetus and mother starts with the onset of placental attachment to the uterus. the cotyledons and caruncles are the two anatomically distinct structures that play a crucial role in this physiological communication. using agilent gene chip genome microarray, we measured the expression profile of pregnancy cotyledons in comparison to caruncular reminiscence of the uteri in non-pregnant buffalo (bubalus bubalis) for the detection of the early post-pregnancy rapid changes in cel ... | 2018 | 29454089 |
| establishment, growth, proliferation, and gene expression of buffalo (bubalus bubalis) transgenic fetal fibroblasts containing human insulin gene, and production of embryos by handmade cloning using these cells. | the aim of the present study was to compare transgenic cells, containing human insulin gene kept under the control of mammary gland-specific buffalo beta-lactoglobulin promoter, and their counterparts, that is, nontransgenic cells, for examining their potential for the production of embryos following somatic cell nuclear transfer (scnt). the gene construct was delivered into buffalo fetal fibroblasts (bff) by nucleofection following which, the transfected cells were selected by culture in the pr ... | 2018 | 29446977 |
| epigenetic alteration of donor cells with histone deacetylase inhibitor m-carboxycinnamic acid bishydroxymide improves the in vitro developmental competence of buffalo (bubalus bubalis) cloned embryos. | epigenetic reprogramming is an indispensable process during the course of mammalian development, but aberrant in cloned embryos. the aim of this study was to examine the effect of donor cell treatment with histone deacetylase (hdac) inhibitor m-carboxycinnamic acid bishydroxymide (cbha) on cloned embryo development and establish its optimal concentration. different concentrations of cbha (2.5, 5.0, 10.0, and 20.0 μm) were used to treat buffalo adult fibroblast cells for 24 hours and effect on ce ... | 2018 | 29412736 |
| hemotropic mycoplasmas infection in water buffaloes (bubalus bubalis) from northeastern brazil. | two species of hemotropic mycoplasmas (hm) are known to infect large domestic ruminants, mycoplasma wenyonii and 'candidatus mycoplasma haemobos'. although hm has been described in cattle worldwide, data in water buffaloes (bubalus bubalis) remain scarce. accordingly, the aim was to determine the occurrence of hm in water buffaloes from northeastern brazil. a total of 101/290 (34.83%) buffaloes were positive for hm (16 m. wenyonii alone, 6 'ca. m. haemobos' alone and 79 both). this was the first ... | 2018 | 29406279 |
| prevalence of foot and mouth disease virus in apparently healthy buffaloes brought to islamabad slaughterhouse in pakistan. | this study was conducted to determine the sero-prevalence of foot and mouth disease (fmd) in buffaloes (bubalus bubalis) slaughtered at islamabad slaughterhouse, pakistan. sera and oro-pharyngeal (op) fluid were collected from 630 healthy buffaloes. these animals originated from various livestock markets from 14 districts of punjab. the age of animals ranged from 1 to 15 years. sera were analyzed using fmd virus (fmdv) nonstructural proteins (nsp) and indirect elisa. the sero-prevalence of fmd w ... | 2018 | 33601788 |
| trends in the quality and hygiene parameters of bulk italian mediterranean buffalo (bubalus bubalis) milk: a three year study. | buffalo milk represents an indispensable source of nourishment in many parts of the world and it is the second most consumed milk worldwide. buffalo milk is actually used for the production of many dairy products such as pasteurized or concentrated milk, butter, yogurt, ice-cream, dehydrated milk products and cheeses. due to its high nutritional value and the presence of natural bioactive substances, buffalo milk can also provide health benefits to consumers. in italy, buffalo milk is used mainl ... | 2018 | 28961355 |
| assessment of a specifically developed bullet casing gun for the stunning of water buffaloes. | water buffaloes and cattle differ considerably with respect to the anatomy of the head. as a result, captive bolt stunners often fail to reliably produce adequate loss of consciousness in water buffaloes and, thus, do not fulfill animal welfare requirements. the goal of the present study was to assess and validate a new stunning device for water buffaloes meeting animal welfare and occupational safety requirements. the newly designed bullet casing gun uses .357mag/10.2g hollow point bullets and ... | 2018 | 28938106 |
| fasciola hepatica infection in water buffalo bubalus bubalis in three provinces of the nile delta, egypt: a cross-sectional study. | a cross-sectional study was conducted to estimate the prevalence of fasciola hepatica (f. hepatica) infection in water buffalo (bubalus bubalis) in alexandria, beheira, and kafr el-sheikh governorates (provinces) of the nile delta in egypt and to identify the underlying risk factors associated with the infection. a total of 29 farms (10 in alexandria, 10 in beheira, and 9 in kafr el-sheikh) were randomly selected and all the buffaloes that resided on these farms from 21 february 2015 to 20 febru ... | 2018 | 29151443 |
| effects of preovulatory follicle size on estradiol concentrations, corpus luteum diameter, progesterone concentrations and subsequent pregnancy rate in buffalo cows (bubalus bubalis). | the present study was undertaken to investigate the effects of preovulatory follicle (pof) size on estradiol concentrations, luteal profile (cl diameter and progesterone concentration) and subsequent pregnancy rate in murrah buffalo cows. the buffalo cows (n = 49) were synchronized for estrus by two doses of pgf2α given 11 days apart. the buffalo cows were inseminated during standing estrus and again after 24 h. ovaries were scanned at estrus and 24 h intervals until ovulation, thereafter on day ... | 2018 | 29128702 |
| partial deoxygenation of extender improves sperm quality, reduces lipid peroxidation and reactive oxygen species during cryopreservation of buffalo (bubalus bubalis) semen. | the present study was designed to investigate the effect of partial deoxygenation of extender on sperm quality, lipid peroxidation (lpo) and reactive oxygen species (ros) in buffalo (bubalus bubalis) during cryopreservation of semen. semen extender was prepared freshly and split into three sub-extenders [extender i: control (non-deoxygenated), extender ii (partially deoxygenated by using ln2 flushing) and extender iii (partially deoxygenated mechanically by vacuum pump)]. amounts of dissolved ox ... | 2018 | 29258709 |
| short communication: stall occupancy behavior of nili ravi buffaloes (bubalus bubalis) when first introduced to freestall housing. | the aim of the present study was to assess stall occupancy of nili ravi buffaloes when first introduced to freestall housing. thirty nili ravi buffaloes of different parity, weight, and pregnancy status were enrolled from the dairy farm of the university of veterinary and animal sciences, lahore, pakistan. all of the enrolled animals had no previous experience with freestalls. the selected buffaloes were introduced to the freestall shed. during an adaptation period of 7 d, all buffaloes had 24 h ... | 2018 | 29224871 |
| correction: impact of heat stress on cellular and transcriptional adaptation of mammary epithelial cells in riverine buffalo (bubalus bubalis). | [this corrects the article doi: 10.1371/journal.pone.0157237.]. | 2018 | 29324889 |
| significance of clinical observations and biochemical alterations in buffalo calves with dietary abomasal impaction. | the present study aimed to throw light on the clinical characteristics of abomasal impaction in buffalo calves and its associated biochemical alterations. for this reason, a total of 20 male buffalo calves (bubalus bubalis) with abomasal impaction were studied. the investigated calves were at 6 to 12 months of age and were belonged to three private farms in dakahlia governorate besides sporadic cases admitted to the veterinary teaching hospital, faculty of veterinary medicine, mansoura universit ... | 2018 | 29291740 |
| incubation of spermatozoa with anandamide prior to cryopreservation reduces cryocapacitation and improves post-thaw sperm quality in the water buffalo (bubalus bubalis). | anandamide (aea), an endocannabinoid, has been shown to reduce capacitation and acrosomal exocytosis in human spermatozoa. because buffalo spermatozoa are highly susceptible to cryopreservation induced damage, aea was assessed as to whether it could protect spermatozoa from cryo-damage. six ejaculates from six murrah buffalo bulls (total 36 ejaculates) were utilized for the study. each ejaculate was divided into four aliquots; spermatozoa in aliquot 1 were extended in tris-citrate-egg yolk and f ... | 2018 | 29277315 |
| a fast and reliable real-time pcr method for detection of ten animal species in meat products. | species substitution in meat products is a common problem reported worldwide. this type of food fraud is, typically, an intentional act for economic gain, using sources of low-priced meats in high-value meat products. consequences include economic, health, and religious concerns. highly sensitive and efficient techniques are thus required to detect meat species. this paper describes a method based on real-time pcr to detect 10 animal species (bos taurus, sus scrofa, ovis aries, capra hircus, gal ... | 2018 | 29377112 |
| spatial-temporal and phylogeographic characterization of trypanosoma spp. in cattle (bos taurus) and buffaloes (bubalus bubalis) reveals transmission dynamics of these parasites in colombia. | animal trypanosomiasis (at) is one of the most important problems in the colombian livestock industry reducing its production around 30%. caribbean and orinoquia regions play a significant role in the development of this industry, having about 6.9 million cattle and 113,000 buffaloes. considering the paucity in studies to understand the epidemiological features and control of at in colombia, the present study reports the seasonal transmission patterns and phylogeographic traits of the causal age ... | 2018 | 29279084 |
| species-specific and differential expression of bsp-5 and other bsp variants in normozoospermic and asthenozoospermic buffalo (bubalus bubalis) and cattle (bos taurus) seminal plasma. | binder of sperm-5 (bsp-5) is one of the fertility-associated proteins of cattle seminal plasma. binding of sperm to the oviductal epithelium is mediated by bsp group of proteins. however, it is not clear, whether this protein is also involved in sperm motility. in the present study, attempts were made to characterize bsp-5 protein in both normozoospermic (ns) and asthenozoospermic (as) murrah buffalo (n = 18; bubalus bubalis), holstein friesian (n = 8, bos taurus) and jersey cattle (n = 8; bos t ... | 2018 | 29100133 |
| global proteomic analysis of water buffalo (bubalus bubalis) saliva at different stages of estrous cycle using high throughput mass spectrometry. | accurate and efficient detection of estrus is one of the major constraints for exploitation of the production potential of buffalo owing to its poor manifestation of estrus signs, seasonal differences in expression and higher incidences of silent estrus (29%). the current study focused on identification of estrus specific candidate proteins in saliva of buffaloes. estrus was detected based on behavioral signs in response to the teaser and changes in reproductive organs and confirmed by per-recta ... | 2018 | 29334660 |
| comparison of three different extenders on murrah buffaloes (bubalus bubalis) semen freezability. | the use of frozen semen for artificial insemination is the main approach utilised for the genetic improvement of most domesticated species. the advantages include lower transportation costs, continuous availability of semen, fewer occurrences of sexually transmitted diseases and the incorporation of desirable genes in a relatively short amount of time. nevertheless, the use of frozen semen in buffalo herds remains limited due to the loss of sperm quality when buffalo semen is frozen. so, the goa ... | 2018 | 28488738 |
| in-gel and offgel-based proteomic approach for authentication of meat species from minced meat and meat products. | fraudulent mislabelling of processed meat products on a global scale that cannot be detected using conventional techniques necessitates sensitive, robust and accurate methods of meat authentication to ensure food safety and public health. in the present study, we developed an in-gel (two-dimensional gel electrophoresis, 2de) and offgel-based proteomic method for authenticating raw and cooked water buffalo (bubalus bubalis), sheep (ovis aries) and goat (caprus hircus) meat and their mixes. | 2018 | 28737240 |
| biochemical analysis of uterine fluid for identification of indicators for subclinical endometritis in the water buffalo (bubalus bubalis). | alterations in biochemical constituents of uterine fluid have been suggested for diagnosis of subclinical uterine infection in the bovine. this study was undertaken to investigate whether uterine fluid biomolecules could act as tool for diagnosis of subclinical endometritis in the buffalo. uterine fluid samples from normal (n = 22) and subclinical endometritis (n = 18; diagnosed based on uterine cytology)-affected buffaloes were subjected to biochemical analysis. among the different biochemical ... | 2018 | 28804962 |
| transient arrest of germinal vesicle breakdown improved in vitro development potential of buffalo (bubalus bubalis) oocytes. | germinal vesicle breakdown (gvbd) is the first milestone that an oocyte needs to achieve toward completing the maturation and gaining potential to fertilize. significantly lower in vitro embryo production rate in buffaloes can be attributed to heterogeneity of gvbd occurrence among oocytes obtained from abattoir derived ovaries. evidence from our earlier work had suggested that different qualities of buffalo oocytes differ significantly in their timing of gvbd. besides, these oocytes also differ ... | 2018 | 28543358 |
| molecular investigation of anaplasma spp. in domestic and wildlife animals in peninsular malaysia. | anaplasma spp. are gram-negative obligate intracellular, tick-borne bacteria which are of medical and veterinary importance. little information is available on anaplasma infection affecting domestic and wildlife animals in malaysia. this study investigated the presence of anaplasma spp. in the blood samples of domestic and wildlife animals in peninsular malaysia, using polymerase chain reaction (ehr-pcr) assays targeting the 16s rrna gene of anaplasmataceae. high detection rates (60.7% and 59.0% ... | 2018 | 31014863 |
| buffalopox. | buffalopox is a contagious viral disease affecting milch buffaloes (bubalus bubalis) and, rarely, cows. the disease has zoonotic implications, as outbreaks are frequently associated with human infections, particularly in the milkers. buffalopox is associated with high morbidity (80%). the clinical symptoms of the disease are characterized by wartline lesions on the udder, teats, inguinal region, base of the ears, and over the parotid. in the severe form, generalized rash is observed. although th ... | 2018 | 29323851 |
| serological and molecular evaluation of mycobacterium avium subspecies paratuberculosis (johne's disease) infecting riverine-type water buffaloes (bubalus bubalis) in the philippines. | mycobacterium avium subsp. paratuberculosis (map) is the causative agent of johne's disease and a possible cause of crohn's disease in humans. a total of 70 blood and fecal samples were collected from water buffaloes in selected municipalities of nueva ecija for elisa and qpcr assay. results revealed presence of antibodies of map in 3 serum samples for elisa. the qpcr assay was carried out using standard curve method targeting the map specific insertion element is900. results revealed that 10 of ... | 2018 | 30502829 |
| major histocompatibility complex dmα/β genes and their expressional diversity in healthy and diseased water buffalo bubalus bubalis. | the major histocompatibility complex (mhc) categorized into three (i, ii and iii) classes elicits the immunogenic response by presenting exogenous peptides to t cells. the mhc-ii dm is composed of dmα and dmβ, two polypeptide chains, both are encoded by separate mhc genes involved in antigen processing and presentation. despite the acknowledged role of mhc complex in humans, the literature is silent on the organization and expression of these genes in water buffalo bubalus bubalis, an agricultur ... | 2018 | 29614492 |