Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| purification of mrna guanylyltransferase and mrna (guanine-7-) methyltransferase from vaccinia virions. | the sequences m7g(5')pppgm-and m7g(5')pppam-are located at the 5' termini of vaccinia mrnas. two novel enzymatic activities have been purified from vaccinia virus cores which modify the 5' terminus of unmethylated mrna. one activity transfers gmp from gtp to mrna and is designated a gtp: mrna guanylyltransferase. the second activity transfers a methyl group from s-adenosylmethionine to position 7 of the added guanosine and is designated a s-adenosylmethionine: mrna (guanine-7-)methyltransferase. ... | 1975 | 1194286 |
| multiple sclerosis and elevation of cerebrospinal fluid vaccinia virus antibody. | thirty percent of 33 patients with a clinical diagnosis of multiple sclerosis and an elevation of cerebrospinal fluid (csf) immunoglobulin g/albumin ratio greater than 2 standard deviations above the mean had elevation of the csf vaccinia neutralizing antibody titer. elevated titers were found in 1 of 23 patients with non-multiple sclerosis neurologic diseases and in none of the normal control patients. no linear correlation was found between the magnitude of the relative or absolute csf immunog ... | 1975 | 1167412 |
| letter: epidemiology of cowpox. | 1975 | 1167474 | |
| [on the problem of persistence]. | 1975 | 1199199 | |
| [effect of neurotropin on sart stress (stress caused by alteration of rhythms in environmental temperature) in mice and rats]. | we reported previously that by altering the rhythm of environmental temperature (art) the physiological mechanism was changed to an abnormal state which we termed specific art-stress (sart-stress). in the present report, the effects of neurotropin and other tranquilizers on this sart-stress state were investigated. neurotropin is an extract containing many conjugated polysaccarides isolated from the skin or tissues of rabbits which had been inoculated with the living cowpox virus. neurotropin pr ... | 1975 | 1169195 |
| [the effect of trichinella spiralis on the susceptibility and antibody production to vaccinia virus]. | trichinella spiralis exerts an immunodepressive effect on production of antihemagglutinins to vaccinia virus in mice, increases the susceptibility to the virus in mice and rabbits. deaths of mice inoculated with vaccine virus and generalization of the vaccination process in rabbits were observed at 33 days of invasion. | 1975 | 1216835 |
| polyamines in vaccinia virions and polypeptides released from viral cores by acid extraction. | vaccinia virions propagated in the presence of [3h]ornithine were found to contain two labeled polyamines, spermine and spermidine. in complete virions the ratio of radioactively labeled spermine to spermidine was about 1:10, whereas in viral cores the ratio was 2:5. this suggests that some spermidine was preferentially lost during the conversion of virions to cores or that spermidine was present in the virions both inside and outside the core structure. addition of [3h]ornithine to vaccinia vir ... | 1975 | 1185852 |
| antigen-enhanced glucosamine incorporation by peritoneal macrophages in cell-mediated hypersensitivity. i. studies on biology and mechanism. | the interaction between sensitized lymphocytes and specific antigen occurring in classic delayed hypersensitivity causes guinea pig peritoneal macrophages to incorporate increased amounts of glucosamine into tca precipitable, membrane-associated, cell surface material. antigen-induced stimulation of glucosamine also occurred in peritoneal exudate cells (pec) isolated from animals primed for cutaneous basophil hypersensitivity with certain strong antigens (klh, vaccinia virus) in incomplete freun ... | 1975 | 1100724 |
| observations in immunotherapy of lymphoma and melanoma patients. | maintenance of remission solely by repeated bcg vaccinations in seven patients with non-hodgkin's lymphoma who had achieved a complete clinical remission with initial standard therapy has provided sufficient encouragement to begin a randomized clinical trial. in vitro lymphocyte responses to mitogens and ppd used as parameters of cell-mediated immunity have not proved to be of value in predicting early or late recurrence in six pre-trial and trial patients. eight out of twenty-one patients with ... | 1975 | 1102163 |
| messenger activity of rna transcribed in vitro by dna-rna polymerase associated to vaccinia virus cores. | the coding properties of rna transcribed in vitro by purified vaccinia cores have been investigated using krebs ascites tumor cells, l cells, and reticulocyte lysates. six to 10 proteins synthesized in vitro are separated on polyacrylamide gels by electrophoresis in the presence of sodium dodecyl sulfate. their molecular weights vary from 10,000 to 44,000. the electrophoretic behavior of these proteins is similar to that of early proteins isolated from infected l cells. the tryptic peptide analy ... | 1975 | 1170340 |
| [studies on the marker behavior of various vaccinia virus strains]. | 1975 | 1229198 | |
| electron microscopy study of the development of neurovaccinia virus in rabbit blood leucocyte cultures. | in electron microscopy of rabbit blood leucocytes cultured after infection with a large dose of neurovaccinia virus, virus particles can be observed within 24 hours in the cytoplasm of the monocytoid cells (monocytes and macrophages) and occasionally in the polymorphonuclear cells, but never in the lymphocytes. the morphology of the virus particles does not differ from the description of these particles in other types of host cells. the virus replication cycle in the monocytoid cells is slow and ... | 1975 | 1237518 |
| purification of a protein kinase and two phosphate acceptor proteins from vaccinia virions. | a novel protein kinase that requires protamine as an activator to catalyze the phosphorylation of viral acceptor proteins was extracted from vaccinia virus cores with deoxycholate and purified 250-fold by dna-cellulose and deae-cellulose column chromatography. the enzyme has a molecular weight of 62,000 as determined by sucrose gradient sedimentation. two heat-stable phosphate acceptor proteins were extracted from virus particles with a nonionic detergent and purified by heat treatment, precipit ... | 1975 | 1123320 |
| the influence of smallpox revaccination upon the foetus. | serological, virological, anatomo- and histopathological investigations were performed in pregnant women subjected to smallpox revaccination. serological findings were normal; no vaccinia virus was isolated from 210 placentae collected upon therapeutical abortion. anatomopathological investigation of 15 placentae showed various lesions, while the embryo were free from any histopathological changes. the data prove that smallpox immunization does not affect the embryo directly, but only through th ... | 1975 | 1224533 |
| [experimental study of the ability of different strains of vaccinia virus to induce autoantibody formation]. | the intensity of autoantibody production to red blood cell lysate in guinea pigs inoculated with smallpox vaccine prepared from different strains was studied at 7, 14, 21 and 28 days after vaccine inoculation. the dynamics of autoantibody production in the same animals as well as the influence of altered responsiveness of the animals pre-treated with dyphtheria toxin on the intensity of autoantibody production were determined. the autoantibody-inducing activity correlated with reactogenicity of ... | 1975 | 1162949 |
| [susceptibility of mice to infection with vaccinia neuropathogenic strain as related to mice strain, age, sex and weight]. | 1976 | 1256124 | |
| biogenesis of vaccinia: specific inhibition of rapidly labeled host dna in vaccinia inoculated cells. | 1976 | 1274178 | |
| phosphonoacetic acid treatment of shope fibroma and vaccinia virus skin infections in rabbits. | the antiviral efficacy of phosphonoacetic acid (paa) was studied in localized skin lesions of rabbits produced by the intradermal inoculation of vaccinia virus (vv) and of shope fibroma virus (sfv). systemic administration of paa by intraperitoneal injections had no significant effect on the pustular lesions induced by vv or on the benign skin tumors caused by sfv. a complete suppression of the appearance of vv-induced pustular lesions was achieved by 2% paa ointment applied twice daily for 4 da ... | 1976 | 1245751 |
| evaluation of measurements of infectious titers of the vaccinia virus in passaged human embryonic fibroblasts. | measurements of infectivity of the vaccinia virus on 11 cell lines derived from human embryos and passaged several times were analyzed statistically. various cell lines showed different sensitivity and differentiated response in various passages. | 1976 | 1259538 |
| h-2 restriction of virus-specific t-cell-mediated effector functions in vivo. ii. adoptive transfer of delayed-type hypersensitivity to murine lymphocytic choriomeningits virus is restriced by the k and d region of h-2. | in mice, primary footpad swelling after local infection with lymphocytic choriomeningitis virus (lcmv) and delayed-type hypersensitivity (dth) adoptively transferred by lcmv immune lymphocytes are t-cell dependent. nude mice do not develop primary footpad swelling, and t-cell depletion abrogates the capacity to transfer lcmv-specific dth. effector t cells involved in eliciting dose-dependent dth are virus specific in that vaccinia virus-immune lymphocytes could not elicit dth in lcmv-infected mi ... | 1976 | 1085340 |
| use of a restriction endonuclease in analyzing the genomes from two different strains of vaccinia virus. | a restriction endonuclease from haemophilus influenzae (hind iii) specifically cleaved vaccinia dna into 14 fragments. the molecular weights of these fragments were determined by gel electrophoresis and ranged from 0.5 x 10(6) to 30 x 10(6). hind iii digestion of the dna from the wr and cv-1 strains of vaccinia revealed a small molecular difference in one of the resulting fragments. the average molecular weight of the entire vaccinia genome was calculated to be 125 x 10(6). | 1976 | 1086369 |
| rapid lysis of vaccinia virus on neutral sucrose gradients with release of intact dna. | 1976 | 1064285 | |
| structure activity relationships of thiosemicarbazones on vaccinia virus and ibt-dependent mutant. | 1976 | 977525 | |
| large-scale purification of animal viruses in the rk-model zonal ultracentrifuge. iii. semliki forest virus and vaccinia virus. | 1976 | 993215 | |
| inhibition of vaccinia virus growth by the nucleoside analogue 1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide (virazole, ribavirin). | virazole or ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide) inhibits the growth of vaccinia virus at a concentration ode to a certain extent in the presence of virazole, the dna fails to acquire resistance to deoxyribonuclease and virus particles are not formed. reversibility of the antiviral effect occurs when the drug is washed out from the infected cultures or when guanosine at an equimolar concentration is added. | 1976 | 1018177 |
| effect of cytosine, arabinoside, iododeoxyuridine, ethyldeoxyuridine, thiocyanatodeoxyuridine, and ribavirin on tail lesion formation in mice infected with vaccinia virus. | mice infected intravenously with vaccinia virus develop characteristic lesions over the entire tail surface. this experimental virus infection presents a highly sensitive and reliable model for evaluating the antivaccinia activity of antiviral compounds. ara-c (1-beta-d-arabinofuranosylcytosine), ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide), iudr (5-iodo-2'-deoxyuridine) as well as two novel analogs of iudr, etudr (5-ethyl-2'-deoxyuridine), and ncsudr (5-thiocyanato-2'-deoxyur ... | 1976 | 1257248 |
| high-voltage electron microscope study of the release of vaccinia virus from whole cells. | high-voltage (1,000-kv) electron microscope examination of whole bsc-1 cells infected with vaccinia virus at different times after infection revealed the presence of increasing numbers of virions no longer confined to factories but situated along the cell periphery of monolayer cells. stereoscopic images showed each virus enclosed within a membrane-like component of the host cell cytoplasm. viruses within factories appeared to lack similar enclosures. cytochalasin b, but not vinblastine, caused ... | 1976 | 1271521 |
| effect of 5-bromodeoxyuridine on vaccinia virus-induced polypeptide synthesis: selective inhibition of the synthesis of some post-replicative polypeptides. | the effect of 5-bromodeoxyuridine (brdu) on vaccinia virus-induced polypeptide synthesis in bsc-1 cells has been investigated. most virus-induced pre- and post-replicative polypeptides were synthesized in concentrations of 5-bromodeoxyuridine that inhibited virus growth. the synthesis of a few post-replicative polypeptides, however, was severely inhibited under these conditions; included in this group was the precursor of a major core component, polypeptide p4b. a delay in the switch-off of pre- ... | 1976 | 1271530 |
| [our experiences with the 2-stage method of vaccination against smallpox (mva virus pre-vaccination)]. | 1976 | 1272640 | |
| phosphorylation of ribosomal proteins in hela cells infected with vaccinia virus. | 1976 | 1246353 | |
| h-2-like specificities of foreign haplotypes appearing on a mouse sarcoma after vaccinia virus infection. | 1976 | 1250351 | |
| [central activity, antihypertensive action and antiulcerogenic effects of neurotropin]. | central activity, antihypertensive action and antiulcerogenic actions of neurotropin (nsp), an extract isolated from vaccinia virus-innoculated skin or tissues of rabbits were investigated herein. when actions of nsp were examined in isolated muscle preparations by the magnus-method, peristalsis and ach-induced contraction in the small intestine isolated from crayfish were not influenced, peristalsis and ach-induced contraction in the small intestine from mice were slightly accelerated, but adre ... | 1976 | 1035190 |
| mrna guanylyltransferase and mrna (guanine-7-)-methyltransferase from vaccinia virions. donor and acceptor substrate specificites. | characterization of the donor and acceptor specificities of mrna guanylyltransferase and mrna (guanine-7-)-methyltransferase isolated from vaccinia virus cores has enabled us to discriminate between alternative reaction sequences leading to the formation of the 5'-terminal m7g(5')pppn-structure. the mrna guanylyltransferase catalyzes the transfer of a residue of gmp from gtp to acceptors which possess a 5'-terminal diphosphate. a diphosphate-terminated polyribonucleotide is preferred to a mononu ... | 1976 | 1002690 |
| the efficacy of vaccinial immune globulin. a 15-year study. | this survey reports on the use of vaccinial immune globulin in australia over the last 15 years. evidence is produced that vaccinial immune globulin has proven efficacious in the prophylaxis and therapy of complications following smallpox vaccination. an indication of the type and frequency of complications following vaccination is provided. | 1976 | 1007152 |
| purification and location of the major glycoprotein of vaccinia virus. | a glycoprotein component of vaccinia virus was extracted with a non-ionic detergent np-40 (nonidet p-40) and purified by gel chromatography. the single antigen extracted by the detergent had a molecular weight estimated between 100,000 and 200,000 daltons. this glycoprotein was found to contain less than 1% hexosamine which would correspond to 5--10 sugar residues per molecule. antibodies produced against this glycoprotein were able to neutralize vaccinia virus. using immunoelectron microscopy, ... | 1976 | 1026850 |
| nosocomial vaccinia infection. | although hospital-associated spread of vaccinia has been reported in the past, there have been no recent reports. this paper describes hospital-associated spread of vaccinia virus infection, supplies data on the environmental survival of vaccinia virus and offers recommendations for the management of patients with vaccinia that may minimize the hazard of infection in other high-risk patients. | 1976 | 1032226 |
| investigations of delayed-type hypersensitivity in rabbits experimentally infected or sensitised by vaccinia virus. | 1976 | 1032930 | |
| the effect of levamisole on cellular immunity in multiple sclerosis. | the lymphocyte stimulation test has been standardized in a normal human population using four virus cell-associated antigens (vcaa): human embryonic lung cells infected with the lec and norrby strains of measles virus, mumps virus, and vaccinia virus. following 1 week of treatment with the immunopotentiating drug levamisole, a group of multiple sclerosis (ms) patients was found to have increased lymphocyte stimulation responses toward vcaa and increased delayed hypersensitivity responses towards ... | 1976 | 991460 |
| the antiviral activity of isatin beta-thiosemicarbazone derivatives on vaccinia virus infection in mice. | newly synthetized compounds of mannich bases isatin-beta-thiosemicarbazone derivatives: n,n'-bis-(beta-thiosemicarbazone-isatinmethyl)-2-methylpiperazine (tski-vi); n,n'-bis (beta-thiosemicarbazoneisatinmethyl)-cis-2,5-dimethylpiperazine (tski-vii) and n,n'-bis (beta-thiosemicarbazoneisatinmethyl)-trans-2,5-dimethylpiperazine (tski-vii), showed protective effect on mice infected i.c. with neurovaccinia virus. antiviral activity was shown already at doses of 1-25 mg/kg increased with the concentr ... | 1976 | 999472 |
| interaction of vaccinia dna-binding proteins with dna in vitro. | 1976 | 1108411 | |
| [generalized progressive vaccinia in a child with primary humoral and cellular immunodeficiency (author's transl)]. | a case of generalized progressive vaccinia with lethal outcome after smallpox vaccination observed in an 8 months old girl during 1968 is reported. this complication was the first sign of an underlying immune deficiency in this child. the most conspicious findings suggesting a humoral immune defect were an absent serum igm in combination with decreased iga and igg levels. an additional cellular defect was suggested by a generalized hypoplasia of the thymus and the entire lymphatic system as show ... | 1976 | 979964 |
| host-induced influences on the syntheses of defective vaccinia particles. | 1976 | 986721 | |
| disaggregation of vaccinia virus with ultrasonic cleaners. | 1976 | 973801 | |
| cells that express viral antigens but lack h-2 determinants are not lysed by immune thymus-derived lymphocytes but are lysed by other antiviral immune attack mechanisms. | murine f9 teratoma cells do not express major transplantation antigens detectable by either serologic or alloreactive assays of thymus-dependent lymphocytes (t cells). such cells can be infected with lymphocytic choriomeningitis virus or vaccinia virus, do express viral antigens on the cell surface, and can release infectious virus in amounts equivalent to those of other h-2 bearing murine cell lines. immunologic injury of virus-infected f9 cells occurs after the addition of specific antiviral a ... | 1976 | 1086476 |
| h-2 compatibility requirement for virus-specific t-cell-mediated cytolysis. the h-2k structure involved is coded by a single cistron defined by h-2kb mutant mice. | lymphocytic choriomeningitis or vaccinia virus-immune spleen cells of h-2 mutant mice carrying a point mutation in the k region (b6 h-2ba, b6 h-2bf) cannot lyse infected wild-type h-2kb targets and vice versa. yet, cytotoxic t cells specific for infected h-2kba or h-2kbf targets are generated during virus infections as shown by cold target competition experiments. the critical structure for the apparent restriction by the k or d regions of the h-2 gene complex of cytolytic interactions between t ... | 1976 | 1082490 |
| h-2 compatibility requirement for virus-specific t-cell-mediated cytolysis. evaluation of the role of h-2i region and non-h-2 genes in regulating immune response. | lymphocytic choriomeningitis virus (lcmv) and ectromelia virus-specific t-cell-mediated cytotoxicity was assayed in various strain combinations using as targets peritoneal macrophages which have been shown to express ia antigens. virus-specific cytotoxicity was found only in h-2k- or d-region compatible combinations. i-region compatibility was not necessary nor alone sufficient for lysis. six different i-region specificities had no obvious effect on the capacity to generate in vivo specific cyto ... | 1976 | 1085331 |
| [the action of the vaccinia virus upon placenta and fetus in revaccinated pregnants (author's transl)]. | we studied 608 antivariolic revaccinated pregnants in spring 1972 at different ages of gestation. 60 presented spontaneous abortus; in 502 cases there has been performed therapeutical abortion, and 46 pregnants revaccinated after 2 1/2-3 months of gestation continued their pregnancy. pregnancy and birth in revaccinated pregnants which did not abort, evolved without any difference against the witness-sample; the newborns presented in a higher percentage underweight. the absence of the vaccinia-vi ... | 1976 | 970028 |
| modification of the 5' terminus of mrna by an rna (guanine-7-)-methyltransferase from hela cells. | the 5' termini of many viral and cellular mrnas contain sequences of the type m7g(5")pppnm. an rna (guanine-7-)-methyltransferase that specifically methylates the 5'-terminal guanosine residue of rnas ending in the dinucleoside triphosphate g(5')pppn- has been purified from the cytoplasm of hela cells. approximately two-thirds of the methyltransferase activity detected in an assay employing umnethylated vaccinia virus mrna as acceptor was located in the cytoplasm when cells were disrupted by dou ... | 1976 | 956186 |
| treatment of vaccinial keratitis with vidarabine. | vaccinial epithelial keratitis was produced in rabbits. when the therapeutic effect of vidarabine on the experimentally-induced disease was evaluated and compared to the effect of idoxuridine, vidarabine was found to be highly effective, substantially more effective than idoxuridine, and nontoxic to the eye. | 1976 | 949279 |
| transmission of vaccinia virus from vaccinated milkers to cattle. | an infection of cattle by transmission of vaccinia virus from milkers vaccinated against small pox is reported. six vaccinia virus strains could be isolated from the vaccinal lesions localized on the nipples of the udder. serological reactions with samples collected from diseased cows demonstrated the presence of hai antibodies and made evident their kinetics at a 2-week-interval. | 1976 | 1034363 |
| editorial: multiple sclerosis: explanations of cause. | 1976 | 946874 | |
| studies on the new cell line tt-i: viral susceptibility and chromosomal changes related to mycoplasma contamination. | for understanding some biological properties of tt-1 cell line established in our laboratory in 1962, its viral susceptibility and the changes in chromosomes related to mycoplasma contamination were studied. tt-1 cells were susceptible to the infection of picorna (polio type 1, coxsackie b1 and echo type 2), measles, newcastle disease, herpes simplex (type 1), adeno (type 12) and vaccinia viruses. contamination of tt-1 cells with mycoplasma hominis was treated successfully with bottromycin a2 (5 ... | 1976 | 1037533 |
| variability of sequential studies of lymphocyte blastogenesis in normal adults. | sixteen healthy adults had serial studies of delayed-type skin test reactivity and in vitro lymphocyte blastogenesis to several antigens over a period of 7 months. in many subjects blastogenesis varied broadly from month to month without apparent cause. responses to all antigens usually increased or decreased together on sequential testing. blastogenesis to coccidioidin appeared to result largely from cross-reaction with histoplasmin. humoral factors were not demonstrably responsible for these c ... | 1976 | 991450 |
| lymphocyte and macrophage responses after vaccinia virus infections. | using a semimicromethod with washed whole blood, in vitro lymphocyte responses of rabbits to intradermal infection with vaccinia virus was studied. peritoneal exudate macrophages were infected with vaccinia in vitro to determine the time of appearance of activated macrophages. after primary infection, an increase in spontaneous incorporation of thymidine by blood cultures was found as early as 2 days postinfection. this effect was at a maximum at 7 to 10 days, with counts up to 100-fold higher t ... | 1976 | 992867 |
| modification of h-2 antigenic sites by enzymatic treatment influences virus-specific target cell lysis. | vaccinia virus-infected cells were treated enzymatically to remove h-2 antigenic sites. the effect of this procedure on virus-specific cell-mediated cytolysis (cmc) and virus-specific antibody-mediated cytolysis (amc) was tested. due to the inhibition of cellular proteinsynthesis by the vaccinia virus infection, h-2 antigenic sites were not resynthesized while there was a continuous production of viral surface antigens. these cells with a high concentration of viral surface antigens and decrease ... | 1976 | 993596 |
| the effect of methylglyoxal bis(guanylhydrazone) on vaccinia virus replication. | 1976 | 999693 | |
| [a new experimental model of vaccinia infection]. | the pathogenic, infectious, and immunogenic properties of vaccinia virus were studied in various species of small laboratory animals (mice of different ages, syrian hamsters, guinea pigs, white and cotton rats, rabbits). cotton rats were found to be most susceptible to the infection. vaccinia virus in this species causes an acute, frequently lethal infection. the inoculated virus intensively accumulated in parenchymatous organs, and this was accompanied by a marked rise of hemagglutinating and v ... | 1976 | 1007222 |
| [effect of viral vaccines on animal bone marrow cell chromosomes]. | a comparative study on the effect of a number of viral vaccines (live and inactivated vaccinia, poliovirus type ii, measles, rabies vaccines) on chromosomes of mouse bone marrow cells was carried out. most vaccines were found to impair the process of first divisions of these cells after vaccination. live vaccinia vaccine and live fixed rabies virus cause an increase in the rate of structural chromosome aberrations at later intervals, 30-90 days after immunization. the main type of chromosome dis ... | 1976 | 1007225 |
| vaccinia virus: the possibilities of its oncogenicity in humans. | contrary to the earlier generally accepted view that vaccinia virus replicates in the cytoplasm only of suitable target cells, steadily accumulating data show that the viral genome spends a limited period of time in the nucleus. this, together with the many cases where a close association has been suspected or established between skin cancer and vaccination, suggests that vaccinia virus may, under certain yet undefined physiological conditions, act as an oncogenic virus in humans. | 1976 | 1016948 |
| extraction of vaccinia virus and purification of vaccinia elementary body suspension with mafron 11. | 1976 | 1017855 | |
| on the regulation of protein synthesis in vaccinia virus infected cells. | all eukaryotic mrna species show a characteristic individual translational efficiency under conditions of restricted polypeptide chain initiation caused by an increase in the osmolarity of the growth medium. in vaccinia virus infected l cells or hela cells virus mrnas can be grouped into classes on the basis of their relative labelling under standard and hypertonic conditions. under the latter conditions, most of the "early" mrnas possess very high translational efficiencies, most of the "interm ... | 1976 | 1018175 |
| [study of the immunogenic properties of 3 lentogenic strains of the newcastle disease virus isolated in bulgaria]. | three local strains of lentogenic type of the newcastle disease were used to produce live liquid vaccines. these were given per os to broiler chickens in order to test their immunogenicity (first vaccination at the age of 25 days and vaccination when 70 days old). the immunity thus acquired was followed serologically by the titer of the antihemagglutinins and the resistance manifested by the birds at the control infection with a proven lethal dose of a highly virulent velogenic strain of the new ... | 1976 | 1030874 |
| antibodies to vaccinia and measles viruses in multiple sclerosis patients. | a virologic comparison was made of 144 patients with multiple sclerosis, 34 of their healthy siblings, and 40 patients with other neurologic diseases (ond). antibodies in serum and cerebrospinal fluid (csf) to vaccinia and measles viruses were measured, and these were correlated in the multiple sclerosis patients with the clinical characteristics of their disease. the csf antibody to vaccinia virus was more frequent and at a higher titer in multiple sclerosis patients than in either control grou ... | 1976 | 938265 |
| modification of the 5'-terminals of mrnas by viral and cellular enzymes. | 1976 | 798242 | |
| poxvirus dna. ii. replication of vaccinia virus dna in the cytoplasm of hela cells. | 1976 | 936479 | |
| dna ligases of eukaryotes. | 1976 | 782919 | |
| formation of the guanylylated and methylated 5'-terminus of vaccinia virus mrna. | 1976 | 948865 | |
| [research on substances with antiviral activity. v. structural analogs of arginine]. | some arginine analogues were synthesized and tested for antiviral activity. two compounds showed weak activity in vitro against vaccinia virus. | 1976 | 955061 |
| the insertion of dna into vaccinia virus. | cells infected with vaccinia virus in the presence of hydroxyurea (hu), which blocks dna replication, were examined in thin sections by electron microscopy at intervals after removal of hu. dense, fibrillar material was observed at the orifice formed just before closure of the membrane constituting the envelope of the immature form of the virus. it is concluded that synchrony of assembly enabled stages in the condensation and insertion of viral deoxyribonucleoprotein to be observed. the mechanis ... | 1976 | 959819 |
| vaccinia virus reexamined: development and release. | 1976 | 960564 | |
| phosphorylation of myelin basic protein by vaccinia virus cores. | 1976 | 972686 | |
| further investigations on the mode of entry of vaccinia virus into cells. | earlier results indicating that vaccinia virus entered l cells by a process of direct fusion between the virus envelope and the plasma membrane of the cell have been confirmed and extended using immuno-ferritin conjugates to locate virus antigens on the host cell surface. after fusion, components of the virus envelope become rapidly dispersed in the plasma membrane. fusion has also been observed as the predominant mode of entry of vaccinia virus into hela cells. | 1976 | 798024 |
| studies on the possible role of lytic enzymes in the intracellular viral decoating using an in vitro trimming system. | 1976 | 977038 | |
| biogenesis of vaccinia: isolation and characterization of a surface component that elicits antibody suppressing infectivity and cell-cell fusion. | 1976 | 982851 | |
| biogenesis of vaccinia: relationship of the envelope to virus assembly. | 1976 | 982852 | |
| presence of haemagglutinin in the envelope of extracellular vaccinia virus particles. | the relationship of vaccinia haemagglutinin (ha) to extracellular enveloped virus (eev) was examined. eev banded in caesium chloride gradients at a density of 1.23 to 1.24 g/ml coincident with a peak of ha activity. eev of an ha+ vaccinia strain showed greater than 90% adsorption to rooster red blood cells (rbcs) as detected by infectivity and 3h-thymidine labelling whereas intracellular naked virus (inv) of the ha+ strain and eev of an ha- strain failed to show significant adsorption. the adsor ... | 1976 | 986420 |
| determination of the mass of viruses by quantitative electron microscopy. | the photometric method of quantitative determination of dry mass by electron microscopy has been applied to the study of various types of viruses: animal, plant, insect, and bacterial. the method is applicable to all viruses having a mass of 1 x 10-18g or greater. the molecular weight of viruses can be calculated from the mass value by multiplying it by avogadro's number. in comparison to other methods of determining the molecular weight of viruses, sedimentation and diffusion, sedimentation equ ... | 1976 | 189345 |
| survival of some viruses in the sea-water samples collected from the gulf of gdańsk. preliminary studies. | 1976 | 189869 | |
| cell surface antigens induced by herpes simplex virus (hsv). | among the antigens induced by hsv on the surface of infected cells there are antigens binding anti-hsv antibodies by their combining (fab) site (hsv-ag), and others binding antibodies of various specificities, probably through their fc fragment (igr). hsv-ag were demonstrated by cytotoxicity and mixed agglutination tests; igr were made evident by passive hemadsorption. the ability to induce igr is especially characteristic of hsv-1 strains, being absent or limited with hsv-2 strains. igr were no ... | 1976 | 188230 |
| characterization of a virion protein kinase as a virus-specified enzyme. | antibodies which completely inhibited the enzymatic activity of the protein kinase associated with virions of frog virus were obtained by immunization of rabbits with the purified enzyme. this inhibition provided a specific probe for the frog virus protein kinase, since this antiserum had no inhibitory effect on a variety of other protein kinases, including the activity of uninfected cells, or the protein kinase associated with vesicular stomatitis virus or vaccinia virus cultivated in the same ... | 1976 | 178663 |
| langerhans cells at the sites of vaccinia virus inoculation. | langerhans cells in the epidermis at the sites of vaccina virus inoculation were studied with the electron microscope. the cells contained unusually increased numbers of the langerhans cell granules. such abnormal langerhans cells have not been described except for in histiocytosis x. vaccinia virus particles were found in the langerhans cells, where they were located individually or embedded in the granular matrix or in lysosomes. | 1976 | 183611 |
| specific inactivation of herpes simplex virus by silver nitrate at low concentrations and biological activities of the inactivated virus. | the infectivities of herpes simplex virus types 1 and 2 were inactivated by silver nitrate at concentrations of 30 mum or less, which did not affect at all the infectivities of hemagglutinating virus of japan, vesicular stomatitis virus, poliovirus, vaccinia virus, and adenovirus. the inactivated virus retained the capability of adsorbing to the cell, with an adsorption kinetics quite similar to that of intact virus, and of inducing the concanavalin a agglutinability in the infected cells, where ... | 1976 | 185946 |
| growth inhibition of cells in cultures and of vaccinia virus infected hela cells by derivatives of trifluorothymidine. | the effects of 5-trifluoromethyluracil (f3thy), 5-trifluoromethyl-2'-deoxyuridine (f3dthd), f3dthd-5'-p, and f3dthd-5'-methylphosphonate on the growth of hela cells, novikoff hepatoma cells, l5178y mouse leukemia cells, and on the replication of vaccinia virus in hela cells have been determined. f3thy and f3dthd-5'-methylophosphonate were approximately 500-fold and 100-fold less effective, respectively, than f3dthd or f3dthd-5'-p in their inhibition of these cells. f3dthd and f3dthd-5'-p are pot ... | 1976 | 178485 |
| vaccinia virus meningitis in mice after intracerebral inoculation. | the pathogenesis of experimental vaccinia virus infection in weanling mice after intracerebral inoculation was followed with virological, histological, and immunohistological methods. high-dose inoculation, virus spread from brain to thoracic and abdominal viscera probably by an undetected early viremia. virus did rise to detectable levels in blood by day 5 and was found to be associated with the mononuclear cell fraction. by day 12, 30% of the animals had died and no further deaths occurred. ri ... | 1976 | 179952 |
| effective antiviral prophylaxis and therapy by systemic application of human interferon in immunosuppressed monkeys. | rhesus monkeys treated with antilymphocytic globulin were infected with vaccinia virus or with medical lake macaque herpesvirus. at the sites of inoculation of vaccinia virus, animals not treated with interferon developed typical pustulous reactions and then smaller secondary lesions. human leukocyte interferon (5 x 10(5) international units per day) injected intramuscularly, either from one day before to three days after infection or for seven days beginning on the first day of primary reaction ... | 1976 | 180202 |
| rescue of vesicular stomatitis virus from homologous and heterologous interferon-induced resistance in human cell cultures by poxviruses. | in human cell cultures the ability of poxviruses to rescue vesicular stomatitis virus from human interferon-induced resistance was significantly more efficient than the ability to rescue it from simian interferon-induced resistance. the sensitivity of the poxvirus to interferon was not related to its ability to rescue vesicular stomatitis virus. | 1976 | 181327 |
| [production and characterization of some mouse embryo cellular substrates in vitro]. | cell cultures with a different multiplication potential in vitro, depending upon the strain source used, were obtained from mouse embryos belonging to the cva, c57 black a2g and swiss strains. only the swiss 12 culture underwent spontaneous transformation and was carried through more than 50 passages in vitro. the swiss-12 substrate proved not to be contaminated either by viruses or micoplasma. it is less sensitive than other elective cell substrates to infection with attenuated polioviruses, cy ... | 1976 | 183259 |
| the vaccinia virus haemagglutinin: enzyme sensitivity and some physiocochemical properties. | purified vaccina virus haemagglutinin (vha) was found to be sensitive to trypsin both with regard to haemagglutinating capacity and antigenic property. phospholipase c and lipase had no effect on the haemagglutination (ha). both the ha and the antigenic property thus depend on the integrity of the protein part. the loss of ha after treatment with trypsin may be due to breakdown of an essential protein or to secondary rearrangement of the whole structure, including the lipid part. cells infected ... | 1976 | 60873 |
| poxvirus infection of the baboon (papio cynocephalus). | ten serial passages of monkeypox (mpv), vaccinia, variola, and chimpanzeepox (chimp-9) viruses were performed in baboons (papio cynocephalus) via skin scarification. comparisons of clinical and virological results indicate that mpv and vaccinia are very closely related and that variola and chimp-9 viruses are identical. these findings suggest that infections of simians with chimp-9 virus resulted from contact with variola virus, the source of which is still unknown. on the other hand, mpv in mon ... | 1976 | 191209 |
| factors involved in the expression of cowpox virus-specific antigen in sendai virus carrier cells. | formation of cowpox virus-specific cell surface antigen (cpvs-ag) hvj (sendai virus) carrier cells compared to that in parent cells. temperature shifts from 32 to 35 to 37 degrees c for these carrier cultures reduced this enhancing activity, making them equivalent to parent cells. the eclips phase and one-step growth of cpv in the carrier cells were shorter than in normal cells. extracts of carrier cells exhibited a stimulating activity causing the temporary rise and subsequent lowering of cpv i ... | 1976 | 185330 |
| [microbial resistance to formaldehyde. i. comparative quantitative studies in some selected species of vegetative bacteria, bacterial spores, fungi, bacteriophages and viruses]. | the resistence of different microorganisms to formaldehyde was determined. as test objects served gram-negative and gram-positive vegetative germs (klebsiella pneumoniae, pseudomonas aeruginosa, salmonella paratyphi-b, staphylococcus aureus, streptococcus faecalis), bacterial spores (bacillus cereus, bacillus pumilus, bacillus stearothermophilus, bacillus subtilis), fungi (aspergillus niger, candida albicans), bacteriophages (escherichia coli phages, t1, t2, t3), and viruses (adenovirus, poliomy ... | 1976 | 190825 |
| application of the fluorescent antibodies method for rapid diagnosis caused of diseases by pox viruses. | the results of application of the fluorescent antibodies method (mfa) with the diagnostic purpose of detecting pox antigen in specimens from smallpox patients are presented, the method was found highly effective for the examination of material taken directly from the patients (smears) in the early stages of disease (maculo-papular and vesicular). the results of examination of smears from pustular fluid and scabs were not reliable in 44% of cases due to the presence of nonspecific fluorescence of ... | 1976 | 184205 |
| activity of albumin conjugates of 5-fluorodeoxyuridine and cytosine arabinoside on poxviruses as a lysosomotropic approach to antiviral chemotherapy. | 1976 | 186717 | |
| production of interferon in human diploid cell cultures. | eleven human diploid cell lines were compared for their ability to produce interferon in response to newcastle disease virus. sensitivity of the cultures of mm, sindbis vsv and vr viruses was also studied. human cells derived from various tissue of a single embryo responded variably to interferon inducer. using selected viruses interferon production was studied in two systems, in cultures of leukocytes and in human embryonic cells. | 1976 | 187142 |
| antiviral activity of carbopol, a cross-linked polycarboxylate. | carbopol, a polymer of acrylic acid cross-linked with allylsucrose, was found to impart resistance to virus infection in mice which received an intraperitoneal injection of the polycarboxylate 1 or 4 days before either intravenous vaccinia virus challenge or intranasal herpes simplex virus challenge. an interferon-like substance was detected in the blood stream 20-40 hours after the intraperitoneal injection of carbopol. the antiviral activity of this cross-linked polycarboxylate could be due to ... | 1976 | 187146 |
| sensitivity of a radioimmunoassay method for detection of certain viral antibodies in sera and cerebrospinal fluids. | an indirect solid-phase radioimmunoassay (ria) was applied to titration of serum and cerebrospinal fluid (csf) antibodies against a variety of viruses including rubella, mumps, measles, herpes simplex, varicella-zoster, and vaccinia. the test used fixed, virus-infected cells as a source of antigen, and conditions for optimal production of viral antigen were determined for each virus-host cell system. in acute, uncomplicated viral infections, sera taken 2 to 5 days after onset generally had low h ... | 1976 | 187618 |
| [discriminant-analytic structure-effect relationship of qualitative biological data. virostatic effect of isatin-beta-isothiosemicarbazones]. | "yes/no" results from the plaque-diffusion inhibition test concerning the virostatic activity of substituted isatin-beta-isothiosemicarbazones against mengo- and vaccinia-viruses can be described discriminant analytically by means of simple substituent constants. the discriminant analysis permits quantitative structure-activity analysis of qualitative and semi-quantitative test data, thus offering a chance for theoretical synthesis optimization already at the level of rough screening. | 1976 | 185860 |
| an antigenically atypical strain of variola virus. | the kuwait-5-67 strain of variola virus, although indistinguishable from variola virus in its other properties, behaved atypically in immuno-diffusion tests with antivaccinia serum. the precipitation line pattern produced was similar to that of cowpox virus but lacked one of the main precipitation bands given by all other strains of variola, alastrim and vaccinia viruses tested. trypsin treatment of cowpox virus preparations revealed the missing precipitation line but did not do so with the kuwa ... | 1976 | 187725 |
| a-type inclusion markers of wild white and other variola-related poxviruses. | 1976 | 187818 | |
| application of the fluorescent antibodies method for rapid diagnosis caused of diseases by pox viruses. | the results of application of the fluorescent antibodies method (mfa) with the diagnostic purpose of detecting pox antigen in specimens from smallpox patients are presented. the method was found highly effective for the examination of material taken directly from the patients (smears) in the early stages of disease (maculo-papular and vesicular). the results of examination of smears from pustular fluid and scabs were not reliable in 44% of cases due to the presence of nonspecific fluorescence of ... | 1976 | 184207 |