Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| impacts of dietary intake and helminth infection on diversity in growth among schoolchildren in rural south china: a four-year longitudinal study. | the authors carried out six transverse anthropometric, dietary, and parasitological surveys of 274 schoolchildren (138 boys and 136 girls), aged 10-12 years (in 2001), from the dongting lake region in rural south china from 2001 to 2005 to examine intra-population differences in growth. compared to the chinese references, the subject children were judged to be retarded in growth at the population level. comparisons among tertiles classified by the extents of change in weight-for-age z score from ... | 2007 | 17160977 |
| studies on the protective immunity of schistosoma japonicum bivalent dna vaccine encoding sj23 and sj14. | in order to explore the high performance bivalent dna vaccine of schistosoma japonicum, the fatty-acid-binding protein (sj14) and the 23 kda transmembrane protein (sj23) two proteins were selected to construct the dna-based vaccine. it was successful to construct a bivalent dna vaccine using three strategies: the co-expression of two genes, a fusion gene expression and two kinds of plasmids in combination (cocktail vaccine). the bivalent dna was proven to express well in vitro and in vivo by ind ... | 2007 | 17182036 |
| comparison of the kato-katz technique, hatching test and indirect hemagglutination assay (iha) for the diagnosis of schistosoma japonicum infection in china. | the kato-katz technique (duplicate 41.7 mg fecal smears), hatching test and indirect hemagglutination assay (iha) were compared for their ability to detect human schistosoma japonicum infection in two endemic villages (zhonjiang and zhuxi) in rural china. the hatching test (using a nylon bag, and based on about 30 g of feces) and iha are conventional chinese diagnostic methods. in both villages, the trends of prevalences with age and sex were comparable for the different methods. in zhuxi, kato- ... | 2007 | 17188018 |
| th2 cytokines are associated with persistent hepatic fibrosis in human schistosoma japonicum infection. | we conducted a prospective cohort study in leyte, the philippines, among 611 schistosoma japonicum-infected participants 7-30 years old, all of whom were treated with praziquantel at baseline. to detect hepatic fibrosis, abdominal ultrasound was performed at baseline and 12 months after treatment. stool for assessment of s. japonicum infection was collected at baseline and at 3, 6, 9, and 12 months after treatment. cytokines (interleukin [il]-4, il-5, il-10, il-13, tumor necrosis factor- alpha , ... | 2007 | 17191174 |
| conservation of cd4+ t cell-dependent developmental mechanisms in the blood fluke pathogens of humans. | schistosoma blood flukes are trematode parasites with a cosmopolitan distribution that infect over 200 million people globally. we previously showed that schistosoma mansoni growth and development in the mammalian host is dependent on signals from host cd4+ t cells. to gain insight into the mechanisms that underlie this dependence, we sought to determine the evolutionary origins and limits of this aspect of the host-pathogen relationship. by infecting rag-1-/- mice with a range of different schi ... | 2007 | 17196594 |
| insight into the host-parasite interplay by proteomic study of host proteins copurified with the human parasite, schistosoma japonicum. | the tegument proteins of schistosome have attracted the most attention in studies of host-parasite interplay, while the host proteins acting at the host-parasite interface remained largely elusive. here, we undertook a high-throughput proteomic approach to characterize the schistosome-adsorbed host proteins. fifty five distinct host proteins were confidently identified in s. japonicum samples, including cercaria, schistosomula, adults, eggs, and miracidia, together with tegument and eggshell pre ... | 2007 | 17211827 |
| statistical evidence for glm regression parameters: a robust likelihood approach. | when a likelihood ratio is used to measure the strength of evidence for one hypothesis over another, its reliability (i.e. how often it produces misleading evidence) depends on the specification of the working model. when the working model happens to be the 'true' or 'correct' model, the probability of observing strong misleading evidence is low and controllable. but this is not necessarily the case when the working model is misspecified. royall and tsou (j. r. stat. soc., ser. b 2003; 65:391-40 ... | 2007 | 17211856 |
| vaccination against schistosoma japonicum infection by dna vaccine encoding sj22.7 antigen. | to observe the in vitro expression of dna vaccine pcdna3-sj22.7 and its immunological effect in mice, the recombinant plasmid pcdna3-sj22.7 was used to transfect hela cells with liposome-mediated method and the expression of sj22.7 mrna and protein was examined using reverse transcription-polymerase chain reaction, sodium dodecylsulfate-polyacrylamide gel electrophoresis and western blot. then, the ability of pcdna3-sj22.7 to protect against schistosoma japonicum challenge infections was analyze ... | 2007 | 17213956 |
| molecular cloning and characterization of a major egg antigen in paragonimus westermani and its use in elisa for the immunodiagnosis of paragonimiasis. | a recombinant protein of a paragonimus westermani egg antigen was produced and tested as an antigen for the serologic diagnosis of p. westermani infection. the p. westermani egg antigen gene contains a single open reading frame of 966 base pairs encoding 322 amino acids from 5' methionine to the 3' stop codon. the predicted amino acid sequence of this egg antigen was 40, 38, and was 35% identical to heat shock proteins from schistosoma japonicum, schistosoma mansoni, and taenia saginata. the dis ... | 2007 | 17136389 |
| immunological cross-reactivity analysis on recombinant histamine-releasing factors from schistosoma japonicum, clonorchis sinensis, and wistar rat. | studies have demonstrated a declined incidence of allergic disorders in the population with helminthic infection. though several hypotheses have been proposed to explain how helminthic infection protected people against allergies, the underlying mechanisms remain poorly understood. a human histamine-releasing factor (hrf) has been proved to be closely related to the development of allergic disorders and the homologues are ubiquitously expressed in all eukaryotic organisms including parasites. to ... | 2007 | 17024353 |
| effect of temperature on the development of schistosoma japonicum within oncomelania hupensis, and hibernation of o. hupensis. | the objectives of this investigation were to assess the effect of temperature on the development of schistosoma japonicum harboured in oncomelania hupensis and to determine the lowest temperature threshold at which the hibernation of o. hupensis occurs. in the first experiment, adult infection-free o. hupensis, collected from jiangsu province in eastern china, were infected with s. japonicum miracidia and raised at different temperatures under laboratory conditions. the development of miracidia ... | 2007 | 17031698 |
| familial aggregation of human susceptibility to co- and multiple helminth infections in a population from the poyang lake region, china. | human helminthiases are common in china, especially in rural areas where sanitation conditions are poor. co- and multiple infections with helminths are strikingly frequent. a cross-sectional parasitological and questionnaire survey was carried out in a population of 3205 individuals belonging to 498 families from five villages in the poyang lake region, jiangxi province, china, to assess their helminth infection status and to collect information on risk factors for infection. the prevalences for ... | 2007 | 17407778 |
| [bioinformatic identification and analysis of schistosoma japonicum adenine phosphoribosyltransferase]. | to identify adenine phosphoribosyltransferases in schistosoma japonicum and analyze their structural features. | 2007 | 17425969 |
| schistosoma japonicum eggs modulate the activity of cd4+ cd25+ tregs and prevent development of colitis in mice. | crohn's disease (cd) is considered to be caused by a disorder of the immune system and helminth infections may interact with development of the disease. we induced colitis in mice by trinitrobenzenesulfonic acid (tnbs) and observed the effects of intraperitoneally injected eggs of schistosoma japonicum on the course of the disease. the inflammation in the colon was reduced in egg-treated mice and secretion of ifn-gamma (a th1 cytokine) by cultured spleen cells in vitro was greatly suppressed, an ... | 2007 | 17433300 |
| environmental effects on parasitic disease transmission exemplified by schistosomiasis in western china. | environmental effects on the transmission of many parasitic diseases are well recognized, but the role of specific factors like climate and agricultural practices in modulating transmission is seldom characterized quantitatively. based on studies of schistosoma japonicum transmission in irrigated agricultural environments in western china, a mathematical model was used to quantify environmental impacts on transmission intensity. the model was calibrated by using field data from intervention stud ... | 2007 | 17438266 |
| cloning, expression and protective immunity evaluation of the full-length cdna encoding succinate dehydrogenase iron-sulfur protein of schistosoma japonicum. | 1071-bp fragment was obtained from the schistosoma japonicum (chinese strain) adult cdna library after the 3' and 5' ends of the incomplete expression sequence tag (est) of succinate dehydrogenase iron-sulfur protein of schistosoma japonicum (sjsdisp) were amplified by the anchored pcr with 2 pairs of primers designed according to the est of sjsdisp and the sequence of multiclone sites of the library vector. sequence analysis indicated that the fragment was a full-length cdna with a complete ope ... | 2007 | 17447029 |
| hepatic changes in congenital schistosoma japonicum infections in pigs. | the inflammatory response in liver tissue from piglets congenitally infected with schistosoma japonicum was examined at two different timepoints after infection. the piglets, which were the offspring of three sows infected with 9000 s. japonicum cercariae in the 10th week of gestation, were allocated into two groups (n=9 and 17) killed 5 or 11 weeks after birth, respectively. all piglets developed a low level infection,with no significant difference between the groups. inflammatory lesions in th ... | 2007 | 17459408 |
| novel sine families from salmons validate parahucho (salmonidae) as a distinct genus and give evidence that sines can incorporate line-related 3'-tails of other sines. | short interspersed elements (sines) constitute a group of retroposons propagating in the genome via a mechanism of reverse transcription, in which they depend on the enzymatic machinery of long retroposons (lines). over 70 sine families have been described to date from the genomes of various eukaryotes. here, we characterize two novel sines from salmons (actinopterygii: salmonoidei). the first family, termed slmi, was shown to be widespread among all genera of the suborder. these sines have a tr ... | 2007 | 17470437 |
| molecular cloning and identification of a novel clonorchis sinensis gene encoding a tegumental protein. | the tegumental membrane of platyhelminth parasites is of crucial importance for modulation of the host response and parasite survival. a cdna encoding a novel tegumental protein 20.8 kda (tp20.8) was found by large-scale sequencing of a clonorchis sinensis cdna library. this new cdna was 755 bp long containing an open reading frame of 555 bp, which encoded a 20.8-kda protein with an isoelectric point of 4.33. the deduced amino acid sequence exhibits 40 and 37% identity to schistosoma japonicum s ... | 2007 | 17476530 |
| efficacy and safety of drug combinations in the treatment of schistosomiasis, soil-transmitted helminthiasis, lymphatic filariasis and onchocerciasis. | this review concerns the efficacy and safety of combinations of various drugs, including albendazole (alb), diethylcarbamazine (dec), ivermectin (ivm), mebendazole and praziquantel (pzq). there were no significant pharmacokinetic interactions when alb-pzq, alb-dec, alb-ivm or alb-ivm-pzq were co-administered. alb did not add to the cure rate of pzq in the treatment of schistosoma japonicum, s. mansoni and s. haematobium. alb and dec in combination and alone were ineffective against s. haematobiu ... | 2007 | 17481681 |
| clonorchis sinensis: expression, characterization, immunolocalization and serological reactivity of one excretory/secretory antigen-lpap homologue. | from a clonorchis sinensis adult cdna plasmid library, a cdna clone encoding a novel lysophosphatidic acid phosphatase (lpap) homologue was isolated. the predicted molecular weight of putative protein was 48.8 kda and the deduced amino acid sequence had 45%, 32%, and 29% identity with lpap of schistosoma japonicum, danio rerio, and homo sapiens, respectively. prediction of signal peptide and western blot analysis indicated that the cslpap homologue was an excretory-secretory antigen (es antigen) ... | 2007 | 17507009 |
| use of a 44k oligoarray to explore the transcriptome of schistosoma mansoni adult worms. | recent advances in the study of schistosoma mansoni genome and transcriptome have led to a better description of the s. mansoni gene complement. in this work, we report the design and use of a new s. mansoni 60-mer oligonucleotide microarray platform with approximately 44,000 probes, based on all publicly available cdna sequence data for s. mansoni and schistosoma japonicum. the large number of probes combined with the extensive sequence annotation available allowed a comprehensive approach, whe ... | 2007 | 17517391 |
| schistosoma mekongi and schistosoma japonicum: differences in the distribution of eggs in the viscera of mice. | the difference in the distribution of schistosoma eggs in the viscera has not been clearly elucidated in the two closely related species schistosoma japonicum and schistosoma mekongi. in this study, we quantitatively compared the distribution of eggs in mice infected with the two species. in s. mekongi-infected mice, 56.6% to 69.4% of total eggs were found in the distal small intestine 9 to 15 weeks after infection, while in s. japonicum-infected mice, 48.8% to 71.8% of eggs were found in the pr ... | 2007 | 17521955 |
| paeoniflorin inhibits tgf-beta1-mediated collagen production by schistosoma japonicum soluble egg antigen in vitro. | the main pathological characteristics of hepatic fibrosis in schistosomiasis are the proliferation of hepatic stellate cells (hscs) and the deposition of collagen type i (col i) and collagen type iii (col iii). transforming growth factor beta-1 (tgf-beta1) plays an important role in hepatic fibrosis. paeoniflorin (pae) has been reported to have immunoregulatory effects; however, the mechanism of its anti-hepatic fibrosis in s. japonicum has not been elucidated. in the present study, we found tha ... | 2007 | 17524166 |
| amino acid transport in schistosomes: characterization of the permeaseheavy chain sprm1hc. | schistosomes are human parasitic flatworms that constitute an important public health problem globally. adult parasites live in the bloodstream where they import nutrients such as amino acids across their body surface (the tegument). one amino acid transporter, schistosome permease 1 light chain, sprm1lc, a member of the glycoprotein-associated family of transporters (gpaat), has been characterized in schistosomes. only a single member of the slc3 family of glycoproteins that associate with gpaa ... | 2007 | 17545149 |
| tracking the fate of iron in early development of human blood flukes. | iron (fe) is an important trace element found in nearly all organisms, and is used as a cofactor in many biological reactions. one role for fe in some invertebrates is in stabilization of extracellular matrices. the human blood fluke, schistosoma japonicum, is responsible for significant human disease in developing and tropical nations. disease in humans arises from host immunological reaction to parasite eggs that lodge in tissues. schistosomes require fe for development in their hosts, and sto ... | 2007 | 17556009 |
| field comparison of immunodiagnostic and parasitological techniques for the detection of schistosomiasis japonica in the people's republic of china. | a total of 1,811 individuals from two villages located in the areas of china endemic for schistosoma japonicum were analyzed by the kato-katz parasitologic examination, indirect hemagglutination assay (iha), and enzyme-linked immunosorbent assay (elisa). statistical analysis of the results showed the kappa indices ranged from 0.106 to 0.234 between iha and the stool examination and ranged from 0.037 to 0.134 between elisa and the fecal examination. the sensitivity value of the iha was 83.7% in v ... | 2007 | 17556625 |
| making the most of mitochondrial genomes--markers for phylogeny, molecular ecology and barcodes in schistosoma (platyhelminthes: digenea). | an increasing number of complete sequences of mitochondrial (mt) genomes provides the opportunity to optimise the choice of molecular markers for phylogenetic and ecological studies. this is particularly the case where mt genomes from closely related taxa have been sequenced; e.g., within schistosoma. these blood flukes include species that are the causative agents of schistosomiasis, where there has been a need to optimise markers for species and strain recognition. for many phylogenetic and po ... | 2007 | 17570370 |
| vaccination of goats with 31 kda and 32 kda schistosoma japonicum antigens by dna priming and protein boosting. | two schistosoma japonicum vaccine candidate antigens sj 31 and sj 32, which have shown particular promise to induce protective immunity in mice, were used to immunize goats by using a dna priming-protein boosting strategy in present work. dna vaccine formulations of the two antigens (vrsj31 and vrsj32) were produced and injected intramuscularly twice at a 2-week interval and then recombinant proteins (rsj31 and rsj32) together with freund complete adjuvant (fca) were used to boost the goats. the ... | 2007 | 17571462 |
| [cloning, expression and characterization of a gene encoding signal transduction protein wnt4 of schistosoma japonicum]. | wnt proteins together with their downstream effectors forms a set of important signal pathways. the wnt signal pathway is important in a wide variety of development processes including cell growth, cell differentiation, cell polarity and apoptosis. wnt4 is a key regulator of gonadal differentiation in humans and mice, playing a pivotal role in early embryogenesis. with race technique based on a est identified in our lab, a novel gene including a complete open reading frame was cloned and named s ... | 2007 | 17577981 |
| gene discovery for the carcinogenic human liver fluke, opisthorchis viverrini. | cholangiocarcinoma (cca)--cancer of the bile ducts--is associated with chronic infection with the liver fluke, opisthorchis viverrini. despite being the only eukaryote that is designated as a 'class i carcinogen' by the international agency for research on cancer, little is known about its genome. | 2007 | 17587442 |
| [a comparison of therapeutic outcomes in liver fibrosis of murine schistosomiasis japonica with interferon-alpha and interferon-gamma]. | 2007 | 17594819 | |
| metal binding ability of glutathione transferases conserved between two animal species, the vanadium-rich ascidian ascidia sydneiensis samea and the schistosome schistosoma japonicum. | glutathione transferases (gsts) are multifunctional enzymes found in many organisms. we recently identified vanadium-binding gsts, designated asgsts, from the vanadium-rich ascidian, ascidia sydneiensis samea. in this study, the metal-selectivity of asgst-i was investigated. immobilized metal ion affinity chromatography (imac) analysis revealed that asgst-i binds to v(iv), fe(iii), and cu(ii) with high affinity in the following order cu(ii)>v(iv)>fe(iii), and to co(ii), ni(ii), and zn(ii) with l ... | 2007 | 17602839 |
| the schistosoma mansoni transcriptome: an update. | large scale est sequencing projects have been carried out for schistosoma mansoni and schistosoma japonicum. this update will briefly review the most recent accomplishments in the area and discuss the use of est data for the purposes of gene discovery, gene model development, genome annotation and snp analysis. in addition, the use of ests for studying other features of the transcriptome such as splice site and transcription initiation variants will be discussed as well as approaches to assignin ... | 2007 | 17624328 |
| prevalence of schistosoma japonicum infection among animals in fifty villages of samar province, the philippines. | in the philippines, there is a need to understand the contribution of different domestic and wild animals in transmitting schistosoma japonicum infection to humans better. the current study describes variation in animal s. japonicum prevalence across 50 endemic villages of samar province, the philippines. a total of 50 villages were selected, 25 with predominantly rain-fed farms and 25 with some irrigation system. at least 35 cats, dogs, pigs, and water buffaloes each were randomly selected and ... | 2007 | 17627431 |
| [effect of n-acetylcysteine on the egg granuloma in hepatic tissue of mice with schistosomosis japonica]. | to study the effect of n-acetylcysteine (nac) on the egg granuloma in hepatic tissue of mice infected with schistosoma japonicum. | 2007 | 17633826 |
| [observation on the change of anti-s. japonicum antibody level in population migrated from outside embankment to new town]. | to detect the change of the anti-s. japonicum antibody level after people migrated from outside embankment to newly established town. | 2007 | 17633828 |
| assessment of the age-specific disability weight of chronic schistosomiasis japonica. | to estimate the age-specific disability weight of chronic schistosomiasis japonica in china. | 2007 | 17639243 |
| [cloning and fusion expression of adenosine deaminase of schistosoma japonicum]. | to clone and express a new protein adenosine deaminase of schistosoma japonicum (sjada). | 2007 | 17639691 |
| [secreted expression of signaling protein 14-3-3 of schistosoma japonicum in pichia pastoris system with primary evaluation on its antigenicity]. | to express signaling protein sj14-3-3 in pichia pastoris and compare its antigenicity with prokaryotic expression one. | 2007 | 17639692 |
| [studies on mechanism of protective immunity against infection of schistosoma japonicum induced by sj26 gene transfected dendritic cell]. | to explore the mechanism of protective immunity against schistosoma japonicum infection induced by sj26 gene transfected dendritic cell (dc). | 2007 | 17639693 |
| schistosoma mansoni genome: closing in on a final gene set. | the schistosoma mansoni genome sequencing consortium has recently released the latest versions of the genome assembly as well as an automated preliminary gene structure annotation. the combined datasets constitute a vast resource for researchers to exploit in a variety of post-genomic studies with an emphasis of transcriptomic and proteomic tools. here we present an innovative method used for combining diverse sources of evidence including ab initio gene predictions, protein and transcript seque ... | 2007 | 17643433 |
| in vitro and in vivo activities of synthetic trioxolanes against major human schistosome species. | schistosomiasis is a parasitic disease that remains of considerable public health significance in tropical and subtropical environments. since the mainstay of schistosomiasis control is chemotherapy with a single drug, praziquantel, drug resistance is a concern. here, we present new data on the antischistosomal properties of representative synthetic 1,2,4-trioxolanes (ozs). exposure of adult schistosoma mansoni for 24 h to a medium containing 20 mug/ml oz209 reduced worm motor activity, induced ... | 2007 | 17283188 |
| aspartic protease activities of schistosomes cleave mammalian hemoglobins in a host-specific manner. | we examined the efficiency of digestion of hemoglobin from four mammalian species, human, cow, sheep, and horse by acidic extracts of mixed sex adults of schistosoma japonicum and s. mansoni. activity ascribable to aspartic protease(s) from s. japonicum and s. mansoni cleaved human hemoglobin. in addition, aspartic protease activities from s. japonicum cleaved hemoglobin from bovine, sheep, and horse blood more efficiently than did the activity from extracts of s. mansoni. these findings support ... | 2007 | 17294005 |
| antibody isotype responses to paramyosin, a vaccine candidate for schistosomiasis, and their correlations with resistance and fibrosis in patients infected with schistosoma japonicum in leyte, the philippines. | we examined whether antibody isotype responses to paramyosin (pm), a vaccine candidate for schistosomiasis, are associated with age-dependent resistance and pathology in liver fibrosis using human sera collected from 139 individuals infected with schistosoma japonicum in leyte, the philippines. we report that iga and igg3 responses to pm showed a positive correlation with age and that the epitopes responsible were localized predominantly within the n-terminal half of pm. in addition, the igg3 re ... | 2007 | 17297052 |
| associations between specific antibody responses and resistance to reinfection in a senegalese population recently exposed to schistosoma mansoni. | we examined associations between schistosome-specific antibody responses and reinfection in senegalese individuals recently exposed to schistosoma mansoni. the effects of treatment, age, intensity of infection and duration of exposure on schistosome-specific antibody responses were also investigated by comparing immune responses in individuals exposed for less than 3 years with responses in people exposed for more than 8 years. all individuals were bled before treatment as well as 6 and 12 weeks ... | 2007 | 17313515 |
| integrating transcriptome, proteome and glycome analyses of schistosoma biology. | publication of the transcriptomes of schistosoma mansoni and schistosoma japonicum, in conjunction with the sequencing and assembly of their genomes, has generated a comprehensive picture of schistosoma transcriptional and genomic diversity. subsequently, researchers who study conjugal and developmental biology, tegumental composition and larval or egg, secretory and excretory products have used these data, in combination with the latest '-omics' technologies, to extend large-scale screens of th ... | 2007 | 17336161 |
| schistosoma mansoni: expression of fes-like tyrosine kinase smfes in the tegument and terebratorium suggests its involvement in host penetration. | protein tyrosine kinases (ptks) are important molecules in intra- and inter-cellular communication, playing a major role in signal transduction processes. we have previously identified and characterized the molecular structure of a new ptk in schistosoma mansoni, smfes. smfes exhibits the characteristic features of fes/fps protein tyrosine kinase subfamily of which it is the first member described in helminths. herein, we show that genes orthologous to smfes are also present in other schistosoma ... | 2007 | 17350619 |
| radiology corner. answer to last month's radiology case and images: schistosomiasis japonicum involving the liver and colon. | abdominal radiography showed subtle curvilinear densities overlying the left lower quadrant and central within the pelvis (fig. 1a). this was confirmed on intravenous contrast-enhanced abdominopelvic ct to be caused by thin mucosal surface calcifications within the descending and sigmoid colons (fig. 1b, c). incidental note is made of thin right hepatic lobe capsule calcifications (fig. 1d). these colonic and hepatic calcifications are characteristic of schistosomiasis infection of the gastroint ... | 2007 | 17357784 |
| enzymatic and nonenzymatic synthesis of glutathione conjugates: application to the understanding of a parasite's defense system and alternative to the discovery of potent glutathione s-transferase inhibitors. | a primary pathway for metabolism of electrophilic compounds in schistosoma japonicum involves glutathione s-transferase (sjgst)-catalyzed formation of glutathione (gsh) conjugates. as part of a program aimed at gaining a better understanding of the defense system of parasites, a series of aromatic halides (1-8), aliphatic halides (9, 10), epoxides (11-20), alpha,beta-unsaturated esters (21, 22), and alpha,beta-unsaturated amides (23, 24) were prepared, and their participation in glutathione conj ... | 2007 | 17226963 |
| higher serum concentrations of dheas predict improved nutritional status in helminth-infected children, adolescents, and young adults in leyte, the philippines. | pubertal development and associated downmodulation of proinflammatory cytokines may predict improved nutritional status, independent of chronic parasite infections, in developing countries. we enrolled 731 individuals, aged 7-30 y, from leyte, the philippines, where helminth infections and nutritional morbidity are highly prevalent. the following data were collected: venous blood hemoglobin and serum concentrations of ferritin, dehydroepiandrosterone sulfate (dheas), c-reactive protein and proin ... | 2007 | 17237323 |
| synthesis of complex-type glycans derived from parasitic helminths. | chemical syntheses of complex-type glycans derived from the eggs of parasitic helminths, schistosoma mansoni and schistosoma japonicum were achieved. in addition, their analogs, which lack xylose and/or fucose residue(s), are described. these branched sugar chains were synthesized regio- and stereoselectively by using beta-mannosylation, desilylation under high-pressure and glycosylation in frozen solvent as key transformations. | 2007 | 17239358 |
| schistosoma japonicum egg antigens stimulate cd4 cd25 t cells and modulate airway inflammation in a murine model of asthma. | a number of epidemiological and clinical studies have suggested an inverse association between allergy and helminth infection, such as schistosomiasis. therefore, we hypothesize that schistosoma japonicum egg antigens, a type of native antigen, can induce production of cd4(+) cd25(+) t cells with regulatory activity, modulating airway inflammation and inhibiting asthma development. the frequency of cd4(+) cd25(+) t cells was determined by flow cytometry for mice treated with ovalbumin (ova), cd2 ... | 2007 | 17042799 |
| cytokine mrna profiles in pigs exposed prenatally and postnatally to schistosoma japonicum. | the pig is a natural host for schistosoma japonicum and a useful animal model of human infection. the aim of the present study was to assess the differences between the cytokine profiles in prenatally or postnatally s. japonicum exposed pigs. seven prenatally exposed pigs, 7 postnatally exposed pigs and 4 uninfected control pigs were compared 27 weeks post exposure. variables included worm burdens, tissue egg counts, liver pathology and mrna levels of il-2, il-4, il-10, il-12, tnf-alpha, tgf-bet ... | 2007 | 17074293 |
| [impact of people's social behavior on schistosomiasis transmission in the three gorges reservoir area]. | to investigate the changes in natural ecology and social environment after the construction of the dam in the three gorges reservoir area, and to understand the social behavior status and education backgrounds of the local residents, and to analyze the potential impact of these factors on schistosomiasis spreading. | 2007 | 17767880 |
| prevalence, intensity and associated morbidity of schistosoma japonicum infection in the dongting lake region, china. | to determine the prevalence and intensity of schistosoma japonicum infection and associated morbidity, and to estimate the infected human and buffalo populations in the dongting lake region, hunan province, china. | 2007 | 17768500 |
| helminth infections and risk factor analysis among residents in eryuan county, yunnan province, china. | whilst infections with soil-transmitted helminths are common across china, the public-health significance of schistosoma japonicum and food-borne helminths is more focalized. only few studies have investigated the local epidemiology of helminth infections in rural china, including risk factor analysis. we collected stool and blood samples from 3220 individuals, aged 5-88 years, from 35 randomly selected villages in eryuan county, yunnan province, china. stool samples were subjected to the kato-k ... | 2007 | 17719553 |
| intrahepatic cholangiocarcinoma with old infestation of schistosoma japonicum: report of a case. | we report a rare case of intrahepatic cholangiocarcinoma associated with old infestation of schistosoma japonicum. the patient was a 76-year-old japanese man who had lived his childhood in an endemic area of this parasite. he presented with jaundice and computed tomography showed a 4-cm, hypodense tumor in segment viii of the liver. microscopically, the resected mass was composed of well-differentiated adenocarcinoma cells. fibrosis and inflammation were seen around the dilated peripheral portal ... | 2007 | 17879045 |
| diagnosis of schistosomiasis japonica infection coincident with hepatocellular carcinoma by fine-needle aspiration. | schistosomiasis japonica is not an endemic parasite in taiwan. previously, there have been no primarily infected cases reported with the exception of chinese war veterans who had immigrated from mainland china. we reported a 79-year-old patient who was diagnosed as having non-b, non-c liver cirrhosis complicated with a 1.2 x 1.2 x 1.2 cm liver tumor in the s5 of the liver. for a definitive diagnosis, we performed ultrasonographic-guided fine needle (21 gauge) aspiration biopsy. in the smear of l ... | 2007 | 17924411 |
| characterization of a partially protective b-cell epitope within the 62 kda antigen of schistosoma japonicum. | approximately 200 million people worldwide currently suffer from schistosomiasis, one of the most important human parasitic diseases. although an established infection can be treated with anthelminthics and praziquantel, vaccination would be the ideal method for integral control of schistosomiasis. schistosoma mansoni irv-5, recommended as a vaccine candidate by the world health organization/special programme for research and training in tropical diseases, produced high protection in animal mode ... | 2007 | 17928926 |
| individual and village-level study of water contact patterns and schistosoma japonicum infection in mountainous rural china. | to describe the exposure patterns related to schistosomiasis transmission in 10 villages in rural xichang county, sichuan, china. | 2007 | 17956502 |
| immunization of mice with cells from juvenile worms of schistosoma japonicum provides immunoprotection against schistosomiasis. | to validate the protective efficacy against schistosomiasis by immunization with cells from juvenile schistosoma japonicum in a murine model and to analyze possible factors related to protection, in this study, two independent repeated vaccination trials were performed. after three subcutaneous vaccinations, in trial one, in the absence of adjuvant, primary juvenile worm cells (pjcs) from s. japonicum induced remarkable average reductions in worm burden (54.3%), liver eggs per gram (lepg) load ( ... | 2007 | 17973094 |
| artemisinins and synthetic trioxolanes in the treatment of helminth infections. | helminthiases, including schistosomiasis and food-borne trematodiasis, affect millions of people. just a few drugs are used for the treatment and control of these diseases. we review recent in-vitro and in-vivo observations with the artemisinins and synthetic trioxolanes against major trematode infections, update clinical findings, and discuss the potential impact that artemisinin-based combination therapy might have on trematode infections in settings where malaria and helminthiases are co-ende ... | 2007 | 17975411 |
| a cluster-randomized bovine intervention trial against schistosoma japonicum in the people's republic of china: design and baseline results. | we describe the design and report baseline results of a cluster-randomized intervention to determine the importance of bovines for schistosoma japonicum transmission in southern china. the study involves four matched village pairs in hunan and jiangxi provinces, with a village within each pair randomly selected as intervention (human and bovine praziquantel treatment) or control (human praziquantel treatment only). total study population prevalences at baseline were 12.4% (n = 5,390) and 15.2% ( ... | 2007 | 17984344 |
| [effect of schistosome ova on trinitrobenzenesulfonic acid induced colitis in mice]. | to investigate the effects of intraperitoneal injected schistosome ova on tnbs-induced colitis and on the intestinal tlr4 expression in mice. | 2007 | 17988539 |
| application of recombinant sjc26gst for serodiagnosis of schistosoma japonicum infection in water buffalo (bos buffelus). | schistosomiasis japonica is currently the most serious parasitic disease in mainland china and it is estimated that several million people are infected. furthermore, it is also responsible for the deaths of many domestic animals. in order to establish an effective diagnostic method, the gene encoding sjc26gst was cloned and expressed in escherichia coli as a fusion protein with his-tag. the purified resjc26gst was used as an antigen for an enzyme-linked immunosorbent assay (elisa) and for immuno ... | 2007 | 17997225 |
| bursts and horizontal evolution of dna transposons in the speciation of pseudotetraploid salmonids. | several genome duplications have occurred in the evolutionary history of teleost fish. in returning to a stable diploid state, the polyploid genome reorganized, and large portions are lost, while the fish lines evolved to numerous species. large scale transposon movement has been postulated to play an important role in the genome reorganization process. we analyzed the dna sequence of several large loci in salmo salar and other species for the presence of dna transposon families. | 2007 | 18021408 |
| effects of the praziquantel-based control of schistosomiasis japonica in china. | in 2000-2002, the effects of the praziquantel-based control of schistosoma japonicum (in which each subject found infected in the october of each year is given a single dose of the drug) were examined at 20 or 21 surveillance sites across china. the pre- and post-treatment prevalences and intensities of infection were compared at each site and after pooling using a random-effects method. one year after treatment, in the communities that had the higher prevalences of infection (> 10%) before trea ... | 2007 | 18028731 |
| [effectiveness of routinely used assays for the diagnosis of schistosomiasis japonica in the field]. | to evaluate the effectiveness of routinely used assays for schistosomiasis diagnosis in the field. | 2007 | 18038771 |
| [potential risks for transmission of schistosomiasis caused by mobile population in shanghai]. | to understand the potential risk for schistosomiasis transmission caused by introduction of infection source from mobile population in shanghai. | 2007 | 18038772 |
| [production and identification of chicken egg yolk antibodies against soluble egg antigen of schistosoma japonicum]. | to produce and purify egg yolk immunoglobulin against soluble egg antigen (sea) of schistosoma japonicum, and evaluate its specificity and sensitivity. | 2007 | 18038775 |
| [bioinformatics analysis for the structure and function of lactate dehydrogenase from schistosoma japonicum]. | to predict the structure and function of sjldh using bioinformatics method. | 2007 | 18038778 |
| [development and identification of the multiple b cell epitope antigens of schistosoma japonicum]. | to develop multiple b cell epitope antigens of schistosoma japonicum and evaluate their antigenicity. | 2007 | 18038797 |
| analysis of the 5q31 33 locus shows an association between single nucleotide polymorphism variants in the il-5 gene and symptomatic infection with the human blood fluke, schistosoma japonicum. | genetic studies of human susceptibility to schistosoma (blood fluke) infections have previously identified a genetic locus determining infection intensity with the african species, schistosoma mansoni, in the 5q31-33 region of the human genome that is known to contain the th2 immune response cluster, including the genes encoding the il-4, il-5, and il-13 cytokines. these cytokines are key players in inflammatory immune responses and have previously been implicated in human susceptibility to infe ... | 2007 | 18056382 |
| epidemiology of schistosomiasis in the people's republic of china, 2004. | results from the third nationwide cluster sampling survey on the epidemiology of schistosomiasis in the people's republic of china, conducted by the ministry of health in 2004, are presented. a stratified cluster random sampling technique was used, and 239 villages were selected in 7 provinces where schistosoma japonicum remains endemic. a total of 250,987 residents 6-65 years of age were included in the survey. estimated prevalence rates in the provinces of hunan, hubei, jiangxi, anhui, yunnan, ... | 2007 | 18257989 |
| [parasite-origin ige-dependent histamine-releasing factors in inducing histamine release from sensitized mast cells]. | to obtain the recombinant ige-dependent histamine-releasing factors of schistosoma japonicum and clonorchis sinensis (rsjhrf and rcshrf) and to study the effect of recombinant hrfs to induce histamine release from sensitized rat mast cells. | 2007 | 18441893 |
| [endemic situation of schistosomiasis at the national surveillance sites of nanjing, 2006]. | three endemic villages were selected as the national surveillance sites and schistosoma japonicum infection in residents and livestock was investigated in 2006. the positive rate of serological (iha) and stool examinations was 10.06% (307/3,053) and 0.13% (4/3,053) for local residents, 10.8% (7/65) and 0 (0/7) for moving people respectively. no infected livestock was found. | 2007 | 18441901 |
| [cloning, expression of schistosoma japonicum elastase gene and its stage-specific transcription]. | to clone, express and purify schistosoma japonicum elastase-2b gene (sjce-2b), and analyze its stage-specific transcription. and expression. | 2007 | 18441984 |
| [suppression of schistosoma japonicum eggs on tnbs-induced colitis in mice]. | to study the suppression of schistosoma japonicum eggs against the trinitrobenzene sulfonic acid (tnbs)-induced colitis in mice. | 2007 | 18441989 |
| [the effect of gossypol, praziquantel and artemether on recombinant lactate dehydrogenase from schistosoma japonicum]. | to detect the effect of gossypol, praziquantel and artemether on activity of the recombinant lactate dehydrogenase of schistosoma japonicum (rsjldh). | 2007 | 18441994 |
| [construction of phage display cdna library from adult worms of schistosoma japonicum]. | to screen protective antigen genes and construct the t7 phage display library from adult worms of schistosoma japonicum. | 2007 | 18441996 |
| [current status and prospects on candidate vaccines against schistosoma japonicum]. | great progress has been made on vaccine research for schistosomiasis, including those on immune mechanism and schistosoma genome which have made active effect to vaccine development. this paper reviews the progress on the candidate vaccine antigens including protein vaccine, dna vaccine and multivalent vaccine against schistosoma japonicum. | 2007 | 18442002 |
| a chronological research on parasitic endemic disease, "katayama-disease" (schistosomiasis japonica) in hiroshima prefecture in the 20th century (the 3rd communication on the historical research on schistosomiasis japonica in hiroshima prefecture). | a chronological research on the parasitic endemic disease, "katayama disease" in hiroshima prefecture was carried out. the present study was concerned mainly with the documents in the 20th century. it was confirmed that the development of excellent chemotherapeutic agents were not pivotal as a result. the eradication of the vector snails was clearly shown to be responsible for that of the infectious disease. | 2007 | 18548885 |
| enhancement by il-18 of the protective effect of a schistosoma japonicum 26kda gst plasmid dna vaccine in mice. | two recombinant plasmids pvax/sj26gst and pvax/mil-18 containing schistosoma japonicum 26kda gst and murine il-18 were evaluated for their ability to protect mice against s. japonicum challenge. mice were given 2 intramuscular immunizations 3 weeks apart, and challenged with s. japonicum cercariae 4 weeks later. adult worm and egg burdens were determined 48 days post-challenge. all animals vaccinated with pvax/sj26gst alone or with pvax/mil-18 developed specific anti-swap (soluble worm antigen p ... | 2008 | 18562051 |
| schistosoma japonicum: protective immunity induced by schistosomulum-derived cells in a mouse model. | we previously reported that immunization with intact live cells from schistosomula of schistosoma japonicum (s.j) partially protected the kunming strain of mice from challenge infection. in the present work, 2 immune protective experiments were designed to further validate the protective effect induced by this type of vaccine and to optimize the immunization protocol, including the number of inoculations and parasite stages from which immunogenic cells were derived. three antigens derived from 1 ... | 2008 | 18564740 |
| sjtpdb: integrated transcriptome and proteome database and analysis platform for schistosoma japonicum. | schistosoma japonicum is one of the three major blood fluke species, the etiological agents of schistosomiasis which remains a serious public health problem with an estimated 200 million people infected in 76 countries. in recent years, enormous amounts of both transcriptomic and proteomic data of schistosomes have become available, providing information on gene expression profiles for developmental stages and tissues of s. japonicum. here, we establish a public searchable database, termed sjtpd ... | 2008 | 18578888 |
| regulatory role of interleukin-10 and interferon-gamma in severe hepatic central and peripheral fibrosis in humans infected with schistosoma japonicum. | schistosoma japonicum is the most pathogenic agent of hepatosplenic schistosomiasis. it causes fibrosis of the central (centf) and peripheral (perf) portal areas. we investigated whether centf and perf in chinese fishermen infected with s. japonicum were associated with an abnormal production of cytokines and chemokines that, in experimental models, have been implicated in the regulation of fibrosis. | 2008 | 18582197 |
| hepatic ultrasound appearance and colonoscopic finding of schistosomiasis japonica. | 2008 | 18585968 | |
| evaluation of igg-elisa for the diagnosis of schistosoma japonicum in a high prevalence, low intensity endemic area of china. | antibody-based diagnostic methods for detecting infection with schistosoma japonicum have been developed and integrated into the national control program in china; however, the utility of these methods compared with conventional coprological methods remains unclear. in two consecutive years, we compared the performance characteristics of kato-katz with a commercially available enzyme-linked immunosorbent assay (elisa) that detects anti-egg antigen igg antibodies in a high prevalence, low intensi ... | 2008 | 18589392 |
| multiple near-identical genotypes of schistosoma japonicum can occur in snails and have implications for population-genetic analyses. | we genotyped (using 16 or 17 microsatellite loci) numerous adult schistosoma japonicum raised in rabbits exposed to pooled cercariae from small numbers of naturally infected snails from several localities in china. as expected, duplicate multi-locus genotypes (mlgs) were found among these worms. additionally, many more mlgs, often near-identical, were found than snails used as sources of cercariae. explanations for these results include (i) genotyping errors, (ii) development within each infecte ... | 2008 | 18590733 |
| molecular characterization of a calcium-binding protein sjca8 from schistosoma japonicum. | a full-length cdna encoding a cercarial stage-specifically expressed 8-kda calcium-binding protein (sjca8) was isolated from schistosoma japonicum cercarial cdna library using microarray screen. the putative gene coding for sjca8 is of 371 bp with an open reading frame of 69 amino acid (aa). the deduced aa sequence showed 83% identity with the schistosoma mansoni 8-kda cabp, 47% identity with clonorchis sinensis calcium-binding protein, and 38% identity with fasciola hepatica putative calcium-bi ... | 2008 | 18622627 |
| [effect of paeoniflorin on hepatic immunopathogenesis in mice with schistosoma japonicum infection]. | to investigate the mechanism of paeoniflorin in preventing hepatic granuloma formation and fibrosis in mice infected with schistosoma japonicum. | 2008 | 18637577 |
| [cloning, expression and identification of membrane protein tetraspanin 2-a of schistosoma japonicum]. | to clone and express a membrane protein (tetraspanin 2) gene of schistosoma japonicum (sjtsp2). | 2008 | 18637579 |
| [putation, identification and bioinformatics analysis of schistosoma japonicum aldehyde dehydrogenase full coding sequence]. | to acquire the full coding sequence of schistosoma japonicum aldehyde dehydrogenase, and fill the gaps of the partial aldehyde dehydrogenase sequences. | 2008 | 18637581 |
| [effect of igg3 antibody purified from sera of microtus fortis against schistosoma japonicum]. | igg3 antibody reaction to soluble antigens prepared from schistosomula (ssa), adult worms (sawa) and eggs (sea) in laboratory-bred microtus fortis (mf), balb/c mice and kunming (km) mice challenged by cercariae of schistosoma japonicum was detected by indirect elisa. the effect of purified igg3 antibody on in vitro killing schistosomula and protecting mice from infection of s. japonicum was evaluated. the igg3 antibody level in mf against ssa and sawa increased significantly by 79.6 percent and ... | 2008 | 18637582 |
| mri in cerebral schistosomiasis: characteristic nodular enhancement in 33 patients. | objective: the purpose of our study was to describe the characteristic mri appearance of cerebral infection with schistosoma japonicum. conclusion: cerebral infection with s. japonicum can cause a characteristic mri pattern of a large mass comprising multiple intensely enhancing nodules, sometimes with areas of linear enhancement. the typical appearance may be useful for diagnosis in endemic regions and may potentially be useful in cases imported into countries in which the disease is not endemi ... | 2008 | 18647936 |
| schistosoma japonicum infection modulates the development of allergen-induced airway inflammation in mice. | asthma, a chronic inflammatory disorder of the airways, is coordinated by th2 cells in both human asthmatics and animal models of allergic asthma. it has been shown that helminth infections including schistosoma mansoni may modulate atopic diseases including asthma. in the present study, balb/c mice were infected with bisexual and unisexual (male) s. japonicum, respectively, prior to ovalbumin (ova) sensitization and challenge. compared to mice with ova sensitization/challenge alone, s. japonicu ... | 2008 | 18654798 |
| a longitudinal study of comparison of the kato-katz technique and indirect hemagglutination assay (iha) for the detection of schistosomiasis japonica in china, 2001-2006. | from 2001 to 2006, about one-third of the residents aged 5-65 years were selected as the subjects in a schistosome-endemic village located in jiangxi province, china. every 1 year, all participants were analyzed by the kato-katz parasitologic examination and indirect hemagglutination assay (iha). statistical analysis of the results showed the k indices ranged from 0.250 to 0.347 between the two methods. assuming the kato-katz results as the gold standard reference, the specificity of the iha was ... | 2008 | 18675244 |
| schistosoma japonicum: inhibition of mago nashi gene expression by shrna-mediated rna interference. | rna interference (rnai) mediated by short interfering rna (sirna) is a powerful reverse genetics tool and holds enormous therapeutic potential for various diseases, including parasite infections. sirnas bind their complementary mrna and lead to degradation of their specific mrna targets. rnai has been widely used for functional analysis of specific genes in various cells and organisms. in this paper, we tested the potential of silencing the expression of the mago nashi gene in schistosoma japoni ... | 2008 | 18466902 |
| rosiglitazone prevents murine hepatic fibrosis induced by schistosoma japonicum. | to evaluate the effect of rosiglitazone in a murine model of liver fibrosis induced by schistosoma japonicum infection. | 2008 | 18473419 |