Publications

proteome approaches to characterize seed storage proteins related to ditelocentric chromosomes in common wheat (triticum aestivum l.).	changes in protein composition of wheat endosperm proteome were investigated in 39 ditelocentric chromosome lines of common wheat (triticum aestivum l.) cv. chinese spring. two-dimensional gel electrophoresis followed by coomassie brilliant blue staining has resolved a total of 105 protein spots in a gel. quantitative image analysis of protein spots was performed by pdquest. variations in protein spots between the euploid and the 39 ditelocentric lines were evaluated by spot number, appearance, ...	2002	12362333
purification of taxi-like endoxylanase inhibitors from wheat (triticum aestivum l.) whole meal reveals a family of iso-forms.	an affinity chromatography method has been developed for purification of endoxylanase inhibitors concentrated by cation exchange chromatography from wheat whole meal and is based on immobilisation of a bacillus subtilis family 11 endoxylanase on n-hydroxysuccinimide activated sepharose 4 fast flow. when followed by high-resolution cation exchange chromatography, the purification of seven taxis, triticum aestivum l. endoxylanase inhibitors was achieved so extending the number of such proteins kno ...	2002	12365463
frequency of the high-molecular-weight glutenin allele in asian hexaploid wheat (triticum aestivum l.) and the transmission route through which the wheat may have reached japan, the most geographically remote region of wheat production in the world.	the frequency of the glu-d1f allele in japanese, chinese, and other asian hexaploid wheat varieties was analyzed in order to investigate a possible transmission route for hexaploid wheat to the far east, japan. the 1380 published data sets were compared to the results for 1107 hexaploid asian wheat varieties which were determined in this study. the frequency of the glu-d1f allele was clearly different between areas; the allele was present from northern and southern japan, from xinjiang, jiangsu, ...	2002	12405793
resistance to wheat streak mosaic virus in transgenic wheat engineered with the viral coat protein gene.	wheat (triticum aestivum) plants were stably transformed with the coat protein (cp) gene of wheat streak mosaic virus (wsmv) by the biolistic method. eleven independently transformed plant lines were obtained and five were analyzed for gene expression and resistance to wsmv. one line showed high resistance to inoculations of two wsmv strains. this line had milder symptoms and lower virus titer than control plants after inoculation. after infection, new growth did not show symptoms. the observed ...	2002	11874101
genetic and physical mapping of lrk10-like receptor kinase sequences in hexaploid oat (avena sativa l.).	oat receptor-like kinase gene sequences, homologous to the lrk10 gene from wheat (triticum aestivum l.), were mapped in oat (avena sativa l.). pcr primers designed from the wheat lrk10 were used to produce alrk10 from oat. two dna sequences, alrk1a1 and alrk4a5, were produced from primers designed from coding and noncoding regions of alrk10. their use as rflp probes indicated that the kinase genes mapped to four loci on different hexaploid oat 'kanota' x 'ogle' linkage groups (4_12, 5, 6, and 13 ...	2002	11908651
rapid identification of transformed wheat using a half-seed pcr assay.	a simple, nondestructive pcr-based screening method has been developed for identifying putative transgenic soft white winter wheat (triticum aestivum l.) carrying the coat protein gene of wheat streak mosaic virus. removal of the endosperm end of individual seed provided sufficient material for dna extraction and pcr. dna from seed is more free of the secondary, metabolites found in leaf tissue that can inhibit both pcr and restriction digests required for southern analysis. the half-seed pcr as ...	2002	11911660
molecular characterization and chromosomal localization of cytochrome p450 genes involved in the biosynthesis of cyclic hydroxamic acids in hexaploid wheat.	the cyclic hydroxamic acids, 2,4-dihydroxy-1,4-benzoxazin-3-one (diboa) and 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (dimboa), are defensive secondary metabolites found in gramineous plants including wheat, maize and rye. cdnas for five cytochromes p450 (p450s) involved in diboa biosynthesis (cyp71c6, cyp71c7v2, cyp71c8v2, cyp71c9v1 and cyp71c9v2) were isolated from seedlings of hexaploid wheat [( triticum aestivum l. cv. chinese spring (2n=6x=42, genomes aabbdd)] by rt-pcr and screening of ...	2002	11976964
genetic dissection of a major fusarium head blight qtl in tetraploid wheat.	the devastating effect of fusarium head blight (fhb) caused by fusarium graminearum has led to significant financial losses across the upper midwest of the usa. these losses have spurred the need for research in biological, chemical, and genetic control methods for this disease. to date, most of the research on fhb resistance has concentrated on hexaploid wheat (triticum aestivum l.) lines originating from china. other sources of resistance to fhb would be desirable. one other source of resistan ...	2002	11999839
abscisic acid- and cold-induced thaumatin-like protein in winter wheat has an antifungal activity against snow mould, microdochium nivale.	cold acclimation of winter wheat (triticum aestivum l.) seedlings induces accumulation in the apoplast of tatlps that are similar to thaumatin-like proteins (tlps), which are pathogenesis-related proteins. we characterized a cdna of was-3a encoding the major isoform of tatlps from winter wheat cells and showed that was-3a transcripts were markedly increased by treatment with aba and by treatment with elicitors (chitosan, beta-glucan and cell wall fractions of fusarium oxysporum and microdochium ...	2002	12010473
a plant gene up-regulated at rust infection sites.	expression of the fis1 gene from flax (linum usitatissimum) is induced by a compatible rust (melampsora lini) infection. infection of transgenic plants containing a beta-glucuronidase (gus) reporter gene under the control of the fis1 promoter showed that induction is highly localized to those leaf mesophyll cells within and immediately surrounding rust infection sites. the level of induction reflects the extent of fungal growth. in a strong resistance reaction, such as the hypersensitive fleck m ...	2002	12011348
glucosylation activity and complex formation of two classes of reversibly glycosylated polypeptides.	reversibly glycosylated polypeptides (rgps) have been implicated in polysaccharide biosynthesis. in plants, these proteins may function, for example, in cell wall synthesis and/or in synthesis of starch. we have isolated wheat (triticum aestivum) and rice (oryza sativa) rgp cdna clones to study the function of rgps. sequence comparisons showed the existence of two classes of rgp proteins, designated rgp1 and rgp2. glucosylation activity of rgp1 and rgp2 from wheat and rice was studied. after sep ...	2002	12011358
molecular and biochemical characterization of a cold-regulated phosphoethanolamine n-methyltransferase from wheat.	a cdna that encodes a methyltransferase (mt) was cloned from a cold-acclimated wheat (triticum aestivum) cdna library. molecular analysis indicated that the enzyme wpeamt (wheat phosphoethanolamine [p-ea] mt) is a bipartite protein with two separate sets of s-adenosyl-l-met-binding domains, one close to the n-terminal end and the second close to the c-terminal end. the recombinant protein was found to catalyze the three sequential methylations of p-ea to form phosphocholine, a key precursor for ...	2002	12011366
winter-annual pasture as a supplement for beef cows.	in each of two experiments, 120 pregnant beef cows were stratified by body condition score, bw, breed, and age, randomly divided into six groups of 20, and assigned to one of six 5.1-ha bermudagrass (cynodon dactylon [l.] pers.) pastures (two replicates/ treatment) in early january to evaluate the use of winter-annual pasture as a supplement. all cows in exp. 1 and 2 had ad libitum access to bermudagrass/dallisgrass (paspalum dilatatum poir.) hay plus three treatments: 1) a concentrate-based sup ...	2002	12019601
mitochondrial dna sequence divergence among schizaphis graminum (hemiptera: aphididae) clones from cultivated and non-cultivated hosts: haplotype and host associations.	a 1.0 kb region of the mitochondrial cytochrome oxidase subunit i gene from the greenbug aphid, schizaphis graminum (rondani), was sequenced for 24 field collected clones from non-cultivated and cultivated hosts. maximum likelihood, maximum parsimony and neighbour-joining phylogenies were estimated for these clones, plus 12 previously sequenced clones. all three tests produced trees with identical topologies and confirmed the presence of three clades within s. graminum. clones showed no relation ...	2002	12020358
stress-induced protein csp 310: a third uncoupling system in plants.	addition of the cold-stress-related protein csp 310 to mitochondria isolated from winter wheat ( triticum aestivum l. cv. zalarinka), winter rye ( secale cereale l. cv. dymka), maize ( zea mays l. cv. vir 36) and pea ( pisum sativum l. cv. marat) caused an increase in non-phosphorylative respiration. this increase was inhibited by kcn, indicating that the protein is not a cn-resistant alternative oxidase. unlike plant mitochondrial uncoupling proteins such as pump, the uncoupling action of csp 3 ...	2002	12029477
functional and comparative bioinformatic analysis of expressed genes from wheat spikes infected with fusarium graminearum.	fusarium head blight, caused by the fungus fusarium graminearum, is a major disease on wheat (triticum aestivum l.). expressed sequence tags (ests) were used to identify genes expressed during the wheat-f. graminearum interaction. we generated 4,838 ests from a cdna library prepared from spikes of the partially resistant cultivar sumai 3 infected with f. graminearum. these ests were composed of 2,831 singlet (single-copy transcripts) and 715 contigs (multiple-copy transcripts) for a total of 3,5 ...	2002	12036275
male gametophyte development in bread wheat (triticum aestivum l.): molecular, cellular, and biochemical analyses of a sporophytic contribution to pollen wall ontogeny.	bread wheat (hexaploid aabbdd genome; 16 billion basepairs) is a genetically complex, self-pollinating plant with bisexual flowers that produce short-lived pollen. very little is known about the molecular biology of its gametophyte development despite a longstanding interest in hybrid seeds. we present here a comprehensive characterization of three apparently homeologous genes (taa1a, taa1b and taa1c) and demonstrate their anther-specific biochemical function. these eight-exon genes, found at on ...	2002	12061894
molecular and structural analyses of a novel temperature stress-induced lipocalin from wheat and arabidopsis.	two cdnas corresponding to a novel lipocalin were identified from wheat and arabidopsis. the two cdnas designated tatil for triticum aestivum l. temperature-induced lipocalin and attil for arabidopsis thaliana temperature-induced lipocalin encode polypeptides of 190 and 186 amino acids respectively. structure analyses indicated the presence of the three structurally conserved regions that characterize lipocalins. sequence analyses revealed that this novel class of plant lipocalin shares homology ...	2002	12062422
the bioavailability of ferulic acid is governed primarily by the food matrix rather than its metabolism in intestine and liver in rats.	the physiologic importance of ferulic acid (fa), and notably its antioxidant properties, depends upon its availability for absorption and subsequent interaction with target tissues. because fa is widely present in cereals, the aim of the present study was to investigate its intestinal and hepatic metabolism in rats by in situ intestinal perfusion model (from 10 to 50 nmol/min), and its bioavailability in supplemented diets (from 10 to 250 micromol/d) or in a complex cereal matrix, i.e., whole fl ...	2002	12097677
cloning of thioredoxin h reductase and characterization of the thioredoxin reductase-thioredoxin h system from wheat.	thioredoxins h are ubiquitous proteins reduced by nadph- thioredoxin reductase (ntr). they are able to reduce disulphides in target proteins. in monocots, thioredoxins h accumulate at high level in seeds and show a predominant localization in the nucleus of seed cells. these results suggest that the ntr-thioredoxin h system probably plays an important role in seed physiology. to date, the study of this system in monocots is limited by the lack of information about ntr. in the present study, we d ...	2002	12106017
in vitro cytotoxic effect of bread wheat gliadin on the lovo human adenocarcinoma cell line.	the pathogenesis of celiac disease is not completely understood but, although the initial step of the process is still unclear, an altered immune response seems to play a major role. previous studies of the biological properties of gliadin have highlighted its cytotoxic effects, and the aim of this study was to develop an in vitro technique to study them. the lovo (human colon adenocarcinoma) cell line grown in two-dimensional cultures was exposed to different concentrations of digested bread wh ...	2002	12110270
a family 11 xylanase from penicillium funiculosum is strongly inhibited by three wheat xylanase inhibitors.	steady-state kinetic approaches were used to investigate the binding of a novel penicillium funiculosum xylanase, xync, with three known xylanase inhibitor proteins from wheat (triticum aestivum). the xylanase gene (xync) was cloned from a p. funiculosum genomic library and the deduced amino acid sequence of xync exhibited high sequence similarity with fungal family 11 xylanases. xync was overexpressed in p. funiculosum and the product (xync: m(r)=23.6 kda; pi=3.7) purified and shown to efficien ...	2002	12147340
[regulation of fertilizer application on yield and root growth of spring wheat-faba bean intercropping system].	the effects of n and p fertilizer application on the multiple population yield and root growth of spring wheat (triticum aestivum)--spring faba bean(vicia sativa)intercropping system were studied by two field experiments with 0, 150 and 225 kgn.hm-2, three pot and pool experiments with 2 n applications (0, 100 mg n.kg-1 soil) and 3 p applications (0, 100 and 200 mg p.kg-1 soil). the results showed that this intercropping system had a significant advantage of yield and beneficial effects. compare ...	2002	12418251
development of sts markers linked to hessian fly resistance gene h6 in wheat.	hessian fly is one of the world's most destructive insect pests of wheat triticum aestivum l. we have used the combination of near-isogenic lines (nil) and random amplified polymorphic dna (rapd) analysis to screen up to 2,000 primers to identify dna markers that are linked to gene h6 that confers resistance to biotype b of the insect. this screen produced six primers that show polymorphic fragments associated with resistance by h6. we have screened 440 f(2) individuals from a cross of the susce ...	2002	12582491
isolation and characterization of five novel high molecular weight subunit of glutenin genes from triticum timopheevi and aegilops cylindrica.	analysis by sds-page of total protein fractions from single seeds of aegilops cylindrica (genomes c and d) and triticum timopheevi (genomes a and g) showed the presence of three bands corresponding to high molecular weight subunits of glutenin (hmw subunits) in the former and two major bands and a minor band corresponding to hmw subunits in the latter. three ae. cylindrica and two t. timopheevi hmw subunit gene sequences, each comprising the entire coding region, were amplified by polymerase cha ...	2002	12582643
[effect of waterlogging on senescence of flag leaf and root of wheat yangmai].	in the pot culture experiment, winter wheat (triticum aestivum l.) c.v. yanmai 5 was selected to study the effect of long-term water-logging, which was conducted from jointing stage to 20 days after anthesis, on grain weight and membrane lipid peroxidation, and on enzyme activities involving membrane lipid peroxide elimination of wheat leaf and root. the content of mda (malondialdehyde), the product of membrane lipid peroxidation in flag leaf and root under waterlogging increased significantly, ...	2002	12625022
geographical distribution and host preference of common bunt tilletia foetidae (wallr.) liro and tilletia caries (dc) tul. on bread wheat and durum wheat.	based on the morphological characteristics of the ustilospores, the geographical distribution and host preference of common bunt tilletia foetidae and t. caries on bread wheat and durum wheat in syria has been studied. the spore wall structure forms the most feasible and convenient method for differentiation between the two pathogens. accordingly, t. foetidae is the most prevalent pathogen in north syria and the predominant pathogen in the bread wheat. t. caries was widespread in durum wheat in ...	2002	12701441
functional conservation of wheat and rice mlo orthologs in defense modulation to the powdery mildew fungus.	homologs of barley mlo are found in syntenic positions in all three genomes of hexaploid bread wheat, triticum aestivum, and in rice, oryza sativa. candidate wheat orthologs, designated tamlo-a1, tamlo-b1, and tamlo-d1, encode three distinct but highly related proteins that are 88% identical to barley mlo and appear to originate from the three diploid ancestral genomes of wheat. tamlo-b1 and the rice ortholog, osmlo2, are able to complement powdery mildew-resistant barley mlo mutants at the sing ...	2002	12437305
proteomic characterization of wheat amyloplasts using identification of proteins by tandem mass spectrometry.	we describe the initial characterization of the wheat amyloplast proteome, consisting of the identification and classification of 171 proteins. whole amyloplasts and purified amyloplast membranes were prepared from wheat (triticum aestivum). protein extracts were examined by one-dimensional and two-dimensional electrophoresis, followed by high performance liquid chromatography-tandem mass spectrometry of separated proteins. tandem mass spectrometry data of individual peptides was then searched b ...	2002	12362334
gene-containing regions of wheat and the other grass genomes.	deletion line-based high-density physical maps revealed that the wheat (triticum aestivum) genome is partitioned into gene-rich and -poor compartments. available deletion lines have bracketed the gene-containing regions to about 10% of the genome. emerging sequence data suggest that these may further be partitioned into "mini" gene-rich and gene-poor regions. an average of about 10% of each gene-rich region seem to contain genes. sequence analyses in various species suggest that uneven distribut ...	2002	11891237
mapping qtls for grain hardness and puroindoline content in wheat ( triticum aestivum l.).	genes for puroindoline-a (pin-a), puroindoline-b (pin-b) and grain-softness proteins (gsp) have been shown to be linked to the dominant ha locus responsible for the soft texture of the grain. though linkage has been demonstrated of the puroindoline genes to the ha locus, there is no clear evidence that puroindoline content is the product of the gene ha. a segregating population of 115 recombinant inbred lines (rils) originating from a cross between the hexaploid synthetic wheat ( triticum durum ...	2002	12582867
biochemical and genetic characterization of a monomeric storage protein (t1) with an unusually high molecular weight in triticum tauschii.	the protein named t1, present in triticum tauschii, was previously characterized as a high-molecular-weight (hmw) glutenin subunit with a molecular size similar to that of the y-type glutenin subunit-10 of triticum aestivum. this protein was present along with other hmw glutenin subunits named 2(t) and t2, and was considered as part of the same allele at the glu-d (t) 1locus of t. tauschii. this paper describes a re-evaluation of this protein, involving analyses of a collection of 173 accessions ...	2002	12582724
a mycorrhiza-responsive protein in wheat roots.	a small protein, designated myk15, was found to be strongly induced in wheat ( triticum aestivum) roots colonized by the arbuscular mycorrhizal fungus glomus intraradices. this protein, which is most abundant in root fractions characterized by strong mycorrhizal colonization, has been characterized using two-dimensional polyacrylamide gel electrophoresis and microsequencing. it has an apparent molecular mass of 15 kda and an isoelectric point of 4.5. the n-terminal sequence has high similarity t ...	2002	12189477
regulation of sucrose and starch synthesis in wheat (triticum aestivum l.) leaves: role of fructose 2,6-bisphosphate.	fructose 2,6-bisphosphate (f26bp) is a competitive inhibitor of the cytosolic fructose 1,6-bisphosphatase (cytfbpase, ec 3.1.3.11). in spinach (spinacia oleracea l.) leaves it is a significant component of the complex regulatory network that co-ordinates rates of photosynthesis, sucrose synthesis and starch synthesis. however the role of f26bp has only been studied in plants that predominantly store starch in their leaves and its role in other species is not clear. this paper examines the signif ...	2002	12172849
[advances in studies of genome-specific repetitive dna sequences in wheat and related species].	in this paper we review recent advances in studies of several aspects of genome specific repetitive dna sequences in wheat and related species. the available results demonstrate that genome specific repetitive dna sequences are important components of genome specificity in wheat and related species. research on genome specific repetitive dna sequences is essential to the elucidation of genome function. the application of genome specific repetitive dna sequences will aid molecular cytogenetic stu ...	2002	16135457
[chromosome analysis of chongqing thermo-photo sensitive male sterile wheat].	seed gliadin acid-polyacrylamide-gel-electrophoresis (apage) and chromosome c-banding techniques were used to identify chromosome constitution of chongqing thermo-photo sensitive male sterile wheat. results showed that 1bs of the male sterile lines was substituted by 1rs of rye. they were 1b/1r wheat with triticum aestivum cytoplasm.	2002	16118133
[effect of modification of cell calcium status on lectin activity].	effects of oryzalin (10 microm), an inhibitor of microtubule polymerization, on the activity of soluble and cell wall lectins were studied in 7 day-old seedlings of unhardened (23 degrees c) and cold acclimated (7 days at 2-3 degrees c) winter wheat (triticum aestivum l.). seedlings were grown in the presence of 25 microm and 1 mm ca2+, 500 microm verapamil, 250 microm chlorpromazine or without modifiers of calcium status in the medium. inhibitor of the microtubule polymerization inhibitor, like ...	2002	14696259
[genetic mapping of the intergenomic translocated "taigu" genic sterility gene (ms2)].	the "taigu" genic sterility gene ms2 located on the short arm of the 4d chromosome of common wheat (aabbdd) originally incorporated into hexoploid triticale (aabbrr) and durum wheat (aabb) through intergenomic translocation in distant hybridization was introduced back into the genomes of common wheat. the dominant male sterility was expressed normally in the new "taigu" genic sterile wheat carrying the intergenomically translocated ms2, and the female fertility mechanism in its male sterile plan ...	2002	12693100
inheritance of dense spike in diploid wheat and aegilops squarrosa.	the individuals of diploid wheat triticum boeoticum, t. monococcum and t. sinskajae and goatgrass aegilops squarrosa were picked out with screening the dense spike characteristics. the dense-spike accessions were discovered in diploid wheat (t. sinskajae) and ae. squarrosa. inheritance of the dense spike was studied. the trait was found to be controlled by a recessive gene in t. sinskajae and by an incomplete dominant gene in ae. squarrosa. the dosage effect of dominant gene c was detected in in ...	2002	12627833
effect of weed extracts on seedling growth of some varieties of wheat.	allelopathic effect ofavena fatua l., cyperus rotundus l., polygonum hydropiper l., and solanum nigrum l. were examined on seedling growth of certain commonly used varieties of wheat (triticum aestivum l.) in the tarai region of u.p. state. the weed extracts inhibited the length of plumule in all the varieties (100%) with solanum and it was in 12 (92%), 10 (77%) and 06 (46%) varieties with polygonum, avena and cyperus, respectively. in radicle length, it was in 92% with both polygonum and solanu ...	2002	12617313
assessment of the pulp and paper mill effluent on growth, yield and nutrient quality of wheat (triticum aestivum l.).	assessment of agropotentiality of the effluent coming out from century pulp and paper mill, ghanshyamdham, lalkua (uttaranchal) has been made on wheat (triticum aestivum var. up-2329) crop grown in two soils differing in texture with different effluent concentrations. diluted effluent increased the chlorophyll content, plant height, shoot and root biomass, grain yield, protein, carbohydrate and lipid contents in wheat grains, while undiluted effluent caused inhibition in plant growth resulting i ...	2002	12597573
effect of external electrical field on mobile water fraction and physiological processes in wheat (triticum aestivum) leaves.	experiments were conducted to study the effect of external electric field on physiological processes, moisture content and mobile water fraction in wheat leaves. application of electric field increased the stomatal resistance immediately and it continued to increase upto 10 min. photosynthesis and respiration of leaves decreased with the application of external electric field. leaf moisture content and mobile fraction of water measured as relaxation times were marginally increased with electric ...	2002	12597031
use of sampl for a study of dna polymorphism, genetic diversity and possible gene tagging in bread wheat.	selective amplification of microsatellite polymorphic loci (sampl) technology was used in bread wheat for the first time for a study of genetic diversity, genotype identification and gene tagging. the diversity studies involved 55 wheat genotypes and two sampl primer pairs (sampl-6 and sampl-7, each with a m-cag primer), which together gave 43 polymorphic bands out of a total of 87 sampl bands. the average polymorphic information content (pic) of sampl primers was 0.221 and that of sampl markers ...	2002	12582720
characterisation and chromosomal localisation of c-type low-molecular-weight glutenin subunits in the bread wheat cultivar chinese spring.	low-molecular-weight glutenin subunits are classically divided into the b, c and d groups. most attention has been paid to the characterisation of the b and d groups, whereas c subunits, although represented by a large number of protein components, have not been thoroughly characterised, mainly because they tend to separate with the gliadins in many fractionation procedures. here we describe a procedure for obtaining a fraction strongly enriched in c subunits that has allowed us to determine the ...	2002	12582715
isolation of est-derived microsatellite markers for genotyping the a and b genomes of wheat.	genetic variation present in 64 durum wheat accessions was investigated by using three sources of microsatellite (ssr) markers: est-derived ssrs (est-ssrs) and two sources of ssrs isolated from total genomic dna. out of 245 ssr primer pairs screened, 22 est-ssrs and 20 genomic-derived ssrs were polymorphic and used for genotyping. the est-ssr primers produced high quality markers, but had the lowest level of polymorphism (25%) compared to the other two sources of genomic ssr markers (53%). the 4 ...	2002	12582712
spelt-specific alleles in hmw glutenin genes from modern and historical european spelt ( triticum spelta l.).	a partial promoter region of the high-molecular weight (hmw) glutenin genes was studied in two wheat specimens, a 300 year-old spelt ( triticum spelta l.) and an approximately 250 year-old bread wheat ( triticum aestivum l.) from switzerland. sequences were compared to a recent swiss landrace t. spelta'oberkulmer.' the alleles from the historical bread wheat were most similar to those of modern t. aestivumcultivars, whereas in the historical and the recent spelt specific alleles were detected. p ...	2002	12582705
physical location of a hsp70 gene homologue on the centromere of chromosome 1b of wheat ( triticum aestivum l.).	cereal centromeres consist of a complex organization of repetitive dna sequences. several repetitive dna sequences are common amongst members of the triticeae family, and others are unique to particular species. the organization of these repetitive elements and the abundance of other types of dna sequences in cereal centromeres are largely unknown. in this study, we have used wheat-rye translocation lines to physically map 1bl.1rs centromeric breakpoints and molecular probes to obtain further in ...	2002	12582685
production of a new wheat line possessing the 1bl.1rs wheat-rye translocation derived from korean rye cultivar paldanghomil.	the 1bl.1rs translocations between wheat ( triticum aestivum l.) and rye ( secale cereale l.) are widely used in bread wheat breeding programs, but all modern wheat cultivars with the 1bl.1rs have shown genetic vulnerability due to one rye source - a german cultivar, petkus. we have developed, a new 1bl.1rs wheat-rye translocation line from the backcross of the f(1) hybrid of wheat cv. olmil and rye cv. paldanghomil, both cultivars from korea. the gish technique was applied to identify the prese ...	2002	12582683
genetic analysis of vrn-b1 for vernalization requirement by using linked dcaps markers in bread wheat ( triticum aestivum l.).	to identify a molecular marker closely linked to vrn-b1, the vrn-1 ortholog on chromosome 5b, sequence polymorphism at four orthologous rflp loci closely linked to the vrn-1 gene family was analyzed by using near-isogenic lines of "triple dirk." at xwg644, a rflp locus, three types of nucleotide sequence differing by the number of (tg) repeats, two or three times, and base changes were detected. a (tg)(3)-type sequence proved to be specific to chromosome 5b by nulli-tetrasomic analysis, and subs ...	2002	12582660
is the mnr locus of triticeae species the same as the ndh and dia loci?	the menadione reductase (mnr), the nicotinamide adenine dinucleotide dehydrogenase (ndh) and diaphorase (dia) isozymes were studied in the allohexaploid triticum aestivum cv "chinese spring" and in five diploid triticeae species. the mnr1, ndh3and dia1 loci were located on the chromosome arms 3al, 3bl and 3dl of t. aestivum, respectively. these loci were also located on the 3h chromosome of hordeum vulgare cv "betzes", the 3l chromosome of aegilops longissima and the 6rl chromosome arm of secale ...	2002	12582652
the molecular genetic characterization of the 'bobwhite' bread wheat family using aflps and the effect of the t1bl.1rs translocation.	bobwhite is a generic name that refers to all sister lines derived from the cross cm 33203 with the pedigree aurora//kalyan/bluebird/3/woodpecker made by the cimmyt bread wheat program in the early 1970s. individual sister lines can be distinguished by their unique selection history. one of the parents, aurora, contains the t1bl.1rs translocation from rye, and approximately 85% of the sister lines have inherited the translocation. the sister lines demonstrate great variability for agronomic trai ...	2002	12582648
molecular characterization of wheat polyphenol oxidase (ppo).	it is well-established that the enzyme polyphenol oxidase (ppo) is involved in undesirable browning of noodles, chapattis, middle east flat breads and steamed breads. methods for measuring ppo activity have been developed, and the variation of ppo activity among wheat ( triticum aestivum l.) cultivars has been well documented. however, there is no report on the identification and characterization of a wheat ppo gene. pcr performed on wheat genomic dna with oligonucleotide primers designed from c ...	2002	12582641
the effect of a deficiency and a deletion on recombination in chromosome 1bl in wheat.	to test two models of chiasma allocation and the distribution of crossing-over in chromosomes, genetic mapping was performed in normal, deletion and deficiency chromosome arms 1bl of wheat, triticum aestivum l. shortening of the chromosome arm, either by a deletion of the proximal half of the arm or by a deficiency of the terminal quarter of the arm's length, significantly reduced the frequency of multiple crossovers but did not affect the distribution of the distal, presumably the first, crosso ...	2002	12582631
characterisation of polymorphic microsatellite markers from aegilops tauschii and transferability to the d-genome of bread wheat.	microsatellites were isolated from a aegilops tauschii (the d-genome donor of bread wheat) library enriched for various motifs. primers generated from the flanking region of the microsatellites were used successfully to amplify the corresponding loci in the d genome of bread wheat. additional amplification sometimes also occurred from the a and b genomes. the majority of the microsatellites contained (ga)(n) and (gt)(n) motifs. ga and gt repeats appeared to be both more abundant in this library ...	2002	12582627
comparison of homoeologous group-6 short arm physical maps of wheat and barley reveals a similar distribution of recombinogenic and gene-rich regions.	eighty two new loci, mapped with 51 dna clones, were added to the earlier deletion maps of the homoeologous group-6 short arms of hexaploid wheat ( triticum aestivum l. em thell., 2n = 6 x = 42, aabbdd). there are now 41, 56 and 52 loci mapped on deletion maps of 6as, 6bs and 6ds, respectively. the linear order of orthologous loci in all three arms appears to be identical. the majority of the loci are located in the distal one-half of the three arms. there seems to be an increased marker/gene de ...	2002	12582615
genes responding to vernalization in hexaploid wheat.	genotype-specific gene expression in response to vernalization in common wheat was examined by the differential display method. two near-isogenic lines of vrn-a1 ( vrn-a1 for the spring type and vrn-a1 for the winter type) were treated by vernalization of developing embryos in detached-ear cultures. this treatment was effective to promote vrn-a1 genotypes to head at a time equivalent to that of vrn-a1. differential cdna fragments were isolated by the rt-pcr method from embryos subjected to verna ...	2002	12582606
flow karyotyping and chromosome sorting in bread wheat ( triticum aestivum l.).	previously, we reported on the development of procedures for chromosome analysis and sorting using flow cytometry (flow cytogenetics) in bread wheat. that study indicated the possibility of sorting large quantities of intact chromosomes, and their suitability for analysis at the molecular level. however, due to the lack of sufficient differences in size between individual chromosomes, only chromosome 3b could be sorted into a high-purity fraction. the present study aimed to identify wheat stocks ...	2002	12582592
identification of novel low m(r) glutenin subunits in the high quality bread wheat cv salmone and their effects on gluten quality.	southern-blot hybridization with a probe specific for genes encoding for low m(r) glutenin subunits showed that the high quality bread wheat cv salmone contains two dna fragments designated as sf720 and sf750. these fragments were found to occur on the chromosome-1b satellite, and to be associated with the presence of two strongly staining low m(r) glutenin subunits in the two-dimensional a-page x sds-page pattern of cv salmone. comparison of 65 f(6) random lines derived from the cross between c ...	2002	12582560
mixing of maize and wheat genomic dna by somatic hybridization in regenerated sterile maize plants.	intergeneric somatic hybridization was performed between albino maize ( zea mays l.) protoplasts and mesophyll protoplasts of wheat ( triticum aestivum l.) by polyethylene glycol (peg) treatments. none of the parental protoplasts were able to produce green plants without fusion. the maize cells regenerated only rudimentary albino plantlets of limited viability, and the wheat mesophyll protoplasts were unable to divide. peg-mediated fusion treatments resulted in hybrid cells with mixed cytoplasm. ...	2002	12582555
genetic mapping of 66 new microsatellite (ssr) loci in bread wheat.	in hexaploid bread wheat ( triticum aestivum l. em. thell), ten members of the iwmmn ( international wheat microsatellites mapping network) collaborated in extending the microsatellite (ssr = simple sequence repeat) genetic map. among a much larger number of microsatellite primer pairs developed as a part of the wmc ( wheat microsatellite consortium), 58 out of 176 primer pairs tested were found to be polymorphic between the parents of the itmi ( international triticeae mapping initiative) mappi ...	2002	12582546
studies of the transferability of microsatellites derived from triticum tauschii to hexaploid wheat and to diploid related species using amplification, hybridization and sequence comparisons.	hexaploid wheat ( triticum aestivum l em thell) is derived from a complex hybridization procedure involving three diploid species carrying the a, b and d genomes, respectively. we recently isolated microsatellites from a t. tauschii library enriched for various motifs and evaluated the transferability of these markers to several diploid species carrying the a, b or d genomes. all of the primer pairs amplifying more than one locus on bread wheat and half of those giving d-genome-specific loci gav ...	2002	12582487
assessing genetic diversity of wheat ( triticum aestivum l.) germplasm using microsatellite markers.	a set of 24 wheat microsatellite markers, representing at least one marker from each chromosome, was used for the assessment of genetic diversity in 998 accessions of hexaploid bread wheat ( triticum aestivum l.) which originated from 68 countries of five continents. a total of 470 alleles were detected with an average allele number of 18.1 per locus. the highest number of alleles per locus was detected in the b genome with 19.9, compared to 17.4 and 16.5 for genomes a and d, respectively. the l ...	2002	12582483
study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a whitexred grain bread-wheat cross.	in many wheat ( triticum aestivuml.) growing areas, pre-harvest sprouting (phs) may cause important damage, and in particular, it has deleterious effects on bread-making quality. the relationship between phs and grain color is well known and could be due either to the pleiotropic effect of genes controlling red-testa pigmentation ( r) or to linkage between these genes and other genes affecting phs. in the present work, we have studied a population of 194 recombinant inbred lines from the cross b ...	2002	12579426
a strategy for enhancing recombination in proximal regions of chromosomes.	as a rule, recombination in bread wheat (triticum aestivum l.) is low in proximal and high in distal regions of chromosomes. recombination may be enhanced in proximal regions by using deletion (del) chromosomes deficient for a distal part of a chromosome arm. the chromosome del5bl-11 derived from chinese spring (cs) is missing 41% of the distal long arm. this line was made polymorphic by crossing with a stock in which chromosome 5b of cs (5b(cs)) is substituted for chromosome 5b of t. turgidum s ...	2002	12575793
[molecular analysis of leaf-rust resistant introgression lines obtained by crossing hexaploid wheat triticum aestivum with tetraploid wheat triticum timopheevii].	twenty-four triticum eastivum x t. timopheevii hybrid lines developed on the basis of five varieties of common wheat and resistant to leaf rust were analyzed by the use of microsatellite markers specific for hexaploid common wheat t. aestivum. investigation of intervarietal polymorphism of the markers showed that the number of alleles per locus ranged from 1 to 4, depending on the marker (2.5 on average). in t. timopheevii, amplification fragments are produced by 80, 55, and 30% of primers speci ...	2002	12575450
plasmon analysis of triticum (wheat) and aegilops. 2. characterization and classification of 47 plasmons based on their effects on common wheat phenotype.	this article comprises our final remarks on the phenotypic effects of alien plasmons on common wheat. twenty-one vegetative, reproductive, and seed characters of 551 alloplasmic lines of 12 common wheat genotypes with 46 alloplasmons, and as the control, their euplasmic lines were investigated. effects of genotype, plasmon, and their interaction had high statistical significance for all the characters investigated, whereas phenotypic variations attributable to the alien plasmons were relatively ...	2002	12589076
bioregulation of carbohydrate metabolism in relation to source-sink operation during grain-filling phase of growth in wheat.	mobilization of free sugars from vegetative tissues to grain and their transformation to starch in relation to activities of some relevant enzymes during growth and development were investigated in wheat (triticum aestivum l.). vegetative tissues, viz. flag-leaf, flag-leaf sheath, nodes and internodes contained high concentration of free sugars from 70 das to 18 dpa and that was in the order of accumulation--flag-leaf sheath> flag-leaf and internodes > nodes. in these tissues, major portion of 1 ...	2002	12587738
mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat ( triticum aestivum l.).	a set of 114 recombinant inbred lines of the 'international triticeae mapping initiative' mapping population was grown during the seasons 1997, 1998, 1999 and 2000 under several environments. twenty morphological (glume colour, awn colour, waxiness, leaf erectness, peduncle length), agronomical (ear emergence time, flowering time, grain filling time, ear length, plant height, lodging, grain number, thousand-grain-weight, grain weight per ear, grain protein content, winter hardiness) and disease ...	2002	12582918
an estimation of the minimum number of ssr alleles needed to reveal genetic relationships in wheat varieties. i. information from large-scale planted varieties and cornerstone breeding parents in chinese wheat improvement and production.	the genetic relationships of 43 wheat varieties were analyzed with ssr markers. the materials employed included 14 cornerstone breeding parents used before 1980 and another 29 other large-scale planted varieties currently in use in china. a total of 501 different alleles were amplified, including 166 alleles of the a genome, 174 of the b genome and 161 of the d genome. data obtained were used to estimate genetic similarity using the dice coefficient, and dendrograms were constructed using the up ...	2002	12582878
microsatellite analysis of wheat chromosome 2d allows the reconstruction of chromosomal inheritance in pedigrees of breeding programmes.	the dwarfing gene rht8 and the photoperiodic insensitivity gene ppd-d1 are linked on the short arm of chromosome 2d of bread wheat and play an important role in determining the geographic adaptation of modern wheat varieties. the genes are believed to originate from the old japanese variety 'akakomugi' and have been distributed throughout the world by diverse breeding programmes. twelve microsatellite loci previously mapped on wheat chromosome 2d were used for a retrospective analysis of 59 whea ...	2002	12582874
[mechanism and universality of yoda's -3/2 law of self-thinning in plant population].	according to plant modular theory, the yoda's-3/2 law of self-thinning was theoretically rederived in this paper. with the parameters in the expression of yoda's-3/2 law being given biological meanings, this article offered universal and rational explanations for relevant data in literatures that could not be explained before. based on the authors' hypothesis, the experiments on the self-thinning process of spring wheat (triticum aestivum l.) were conducted. individual morphological and populati ...	2002	12561166
[hydrous and photosynthetic adaptations of common and durum wheat to saline stress].	seven varieties of bred wheat and seven varieties of durum wheat were cultivated in three different sites from the area of errachidia (southeastern morocco). these sites differ by the degree of salinity in the irrigation water. results obtained showed that the reduction in leaf area is the principal strategy that makes it possible to attenuate the effects of the reduction in the availability of water under saline stress. bread wheat, which limited the reduction in the leaf area, with the risk to ...	2002	12506723
differential effects of cultivated and wild barley 5h chromosomes on heading characters in wheat-barley chromosome addition lines.	wheat (triticum aestivum l.)-barley (hordeum vulgare l.) chromosome addition lines are possible vehicles for transferring barley genes into wheat. the barley 5h chromosome has genetic effects on the heading characters in wheat-barley addition lines: accelerating narrow-sense earliness, decreasing vernalization requirement and/or increasing photoperiodic sensitivity. to elucidate the effects of different 5h chromosomes under an identical wheat genetic background, two wheat-barley addition lines, ...	2002	12471665
sequence tagged microsatellite profiling (stmp): improved isolation of dna sequence flanking target ssrs.	sequence tagged microsatellite profiling (stmp) enables the rapid development of large numbers of co-dominant dna markers, known as sequence tagged microsatellites (stms). each stm is amplified by pcr using a single primer specific to the conserved dna sequence flanking the microsatellite repeat in combination with a universal primer that anchors to the 5'-ends of the microsatellites. it is also possible to convert stms into conventional microsatellite, or simple sequence repeat (ssr), markers t ...	2002	12466561
bioaccumulation of lanthanum and cerium and their effects on the growth of wheat (triticum aestivum l.) seedlings.	through short-term exposure (7-d exposure), long-term exposure (16-d exposure) and exposure-recovery (7-d exposure + 9-d recovery), the bioaccumulation and distribution of la and ce and their effects on growth of wheat seedlings were studied. addition of la (0.5-25 mg/l) and ce (0.5-25 mg/l) to the culture medium individually and in combination inhibited primary root elongation, reduced the dry weight of roots and shoots and the content of mineral elements (ca, mg, k, cu, zn). the damage increas ...	2002	12143937
[studies on fertility genes and its genetic characters in d2-type cms lines of common wheat].	the kinds of fertility genes and its genetic characters for d2-type cms lines of common wheat were studied, the results showed: (1) for d2-type cms line, there was wide restoring sources and high digree of restoration in common wheat varieties (the restoring degree of 33.61% varieties exceeded 50%), and new cms lines were easily bred (25.21% varieties could maintain male sterility). these characters indicated that d2-type cms line was higher value in applied study, compared with t, k, v-type cms ...	2002	12143315
[development of new pcr markers specific to a thinopyrum intermedium chromosome 2ai-2 and cloning of the st-specific sequences].	to meet the need of selecting translocation lines, some new rapd markers for 2ai-2 chromosome of th. intermedium were identified in the paper. out of 320 rapd primers, 2 specific primers, opo05 and opm04, can amplify respectively a specific band with size of about 650 bp and 1400 bp in the bydv resistant materials containing the chromosome 2ai-2, including th. intermedium, wheat-th. intermedium partial amphipoild zhong 4 awnless, addition lines z1, z2 and z6, f1(z2/wan7107) and substitute line z ...	2002	12143313
[morphology of wheat roots under low-phosphorus stress].	the morphology of root systems of different wheat (triticum aestivum l.) genotypes under low-phosphorus stress were studied to determine the effects of external factors on components of root system and the early morphological indicators related to phosphorus efficiency. the number of root axes and the length of lateral root of p-deficient plant were significantly lower than those of p-sufficient plant. the length of root axis and root system, and the number of lateral roots were sharply increase ...	2002	12132156
proteomic analysis of amphiphilic proteins of hexaploid wheat kernels.	wheat proteins and specially gluten proteins have been well studied and are closely associated with baking products. amphiphilic proteins (proteins that are soluble using nonionic detergent triton x-114 ) also play an important role in wheat quality. some of them, like puroindolines, are lipid binding proteins, and are strongly linked to dough foaming properties and to fine crumb texture. however many amphiphilic proteins are still unknown and both their physiological and technological functions ...	2002	12112842
aluminium triggers malate-independent potassium release via ion channels from the root apex in wheat.	the regulatory mechanisms for the aluminium (al)-induced efflux of k(+) and malate from the root apex of al-resistant wheat ( triticum aestivum l. cv. atlas) were characterized. treatment with 20 mm tetraethylammonium (tea) chloride, a k(+)-channel inhibitor, blocked the al-induced k(+) efflux by 65%, but blocked the al-induced malate efflux only slightly. lanthanum (la(3+)) or ytterbium (yb(3+)) strongly inhibited the k(+) efflux, but slightly increased malate efflux. these lanthanides applied ...	2002	12111222
[effects of the enhanced uv-b radiation on the body cell mitosis of the wheat].	the chromosomal mutation and mitosis of the wheat (triticum aestivum, cv. 93-4736) were observed under the condition of the enhanced ultraviolet-b radiation. the results showed that the number of cells in mitosis of the uv-b treatments was 68.09% of the control, indicating that the uv-b treatment significantly inhibited division of root tip cells in wheat. using t-test at p < or = 0.05, there was a significant difference between the control and the treatments. the enhanced uv-b significantly inc ...	2002	12096633
[genetic analysis on restorer genes of d2-type cms lines of common wheat].	on the basis of genetic characters of d2-type cms lines, restorer lines yi4060, m8003, 6d/6r, gr1, 960789, bao769-22-1 et al. were gained from crossing selection. the results of genetic analysis of f2, f1bc1 population and allelic analysis of restorer genes, successive selection of self fertility from f1 showed that fertility restoration of these restorer lines was controlled by two pair independent major-genes(dominant) and different-dosage minor genes. authors proposed that the two pair indepe ...	2002	12096632
characterization of two non-homoeologous nuclear genes encoding mitochondrial alternative oxidase in common wheat.	mitochondrial alternative oxidase (aox) is the terminal oxidase responsible for cyanide-insensitive and salicylhydroxamic acid-sensitive respiration. we have isolated two non-homoeologous genes (waox1a and waox1c) encoding aox proteins from common wheat (triticum aestivum l.). these two genes were orthologous to rice aox1a and aox1c, and their exon/intron structure was conserved, as it is in most other plant aox genes. southern blot analysis indicated that both waox1a and waox1c were located in ...	2002	12087190
genotypic and nutrition-dependent variation in water use efficiency and photosynthetic activity of leaves in winter wheat (triticum aestivum l.).	effects of high and reduced npk nutrition on the genetic variation of components of water use efficiency at the leaf and whole-plant levels were examined in pot-grown old and modern cultivars of winter wheat (triticum aestivum l.). at the subsequent growth stages, the photosynthetic rate (a), transpiration rate (e), leaf area (la) and gas exchange efficiency (a/e) were measured on fully developed 4th, 5th, penultimate and flag leaves. at the plant canopy level, the total amount of water transpir ...	2002	12080171
a simplified aflp method for fingerprinting of common wheat (triticum aestivum l.) cultivars.	the simplified aflp method was developed and evaluated for identification and genetic diversity studies of wheat cultivars. selective primers exploited in aflp assay based on a single cutting enzyme psti ((psti)aflp) generated total of 111 robust fragments, including 67 (60%) monomorphic and 12 (11%) cultivar-specific markers. average similarity between 15 cultivars was 0.650, and varied from 0.293 ('hope' vs. 'aurora') to 0.865 ('norman' vs. 'hornet'). mean similarities within groups of winter ...	2002	12080170
chelate-assisted phytoextraction of lead from a contaminated soil using wheat (triticum aestivum l.).		2002	12068937
genes encoding plastid acetyl-coa carboxylase and 3-phosphoglycerate kinase of the triticum/aegilops complex and the evolutionary history of polyploid wheat.	the classic wheat evolutionary history is one of adaptive radiation of the diploid triticum/aegilops species (a, s, d), genome convergence and divergence of the tetraploid (triticum turgidum aabb, and triticum timopheevii aagg) and hexaploid (triticum aestivum, aabbdd) species. we analyzed acc-1 (plastid acetyl-coa carboxylase) and pgk-1 (plastid 3-phosphoglycerate kinase) genes to determine phylogenetic relationships among triticum and aegilops species of the wheat lineage and to establish the ...	2002	12060759
[identification, mapping, and application of a chromosome-specific rapd marker from haynaldia villosa].	random amplified polymorphic dna (rapd) analysis was performed on common wheat of chinese spring, addition lines of h. villosa chromosome in cs and h. villosum from different accessions with 100 random 10-base primers. a chromosome-specific polymorphic dna segment for h. villosa, opf02(750), was obtained from all addition lines of h. villosa chromosome in cs and h. villosum which belong to different accessions. the result amplified by primer opf02 of all addition lines of h. villosa chromosome i ...	2002	12043575
[genetic diversity revealed by issr molecular marker in common wheat, spelt, compactum and progeny of recurrent selection].	it is important to estimate the genetic diversity between the parents for improving the heterosis of hybrid wheat. in this study, issr(inter-simple sequence repeat) marker was used to measure the genetic diversity within and among common wheat (triticum aestivum l.), spelt (triticum spelta l.), compactum (triticum compactum host.) and progeny of foreign wheat-based recurrent selection, and the possibility of establishing the new heterotic group was also assessed. forty seven genotypes were used ...	2002	12043574
activation volumes of processes linked to the phototransformation of protochlorophyllide determined by fluorescence spectroscopy at high pressure.	the photochemical activity of nadph:protochlorophyllide oxidoreductase (por) was studied in etiolated wheat (triticum aestivum, l., cult. mv17) leaf homogenates. the kinetics of the transformation of protochlorophyllide into chlorophyllide was detected by fluorescence intensity changes at 690 nm (formation of chlorophyllide) and 655 nm (decay of protochlorophyllide) at 20 degrees c, excited at 440 nm while the pressure was varied between 0.1 and 400 mpa. both kinetics could be fitted by two expo ...	2002	12034465
hordoindolines are associated with a major endosperm-texture qtl in barley (hordeum vulgare).	endosperm texture has a tremendous impact on the end-use quality of wheat (triticum aestivum l.). cultivars of barley (hordeum vulgare l.), a close relative of wheat, also vary measurably in grain hardness. however, in contrast to wheat, little is known about the genetic control of barley grain hardness. puroindolines are endosperm-specific proteins found in wheat and its relatives. in wheat, puroindoline sequence variation controls the majority of wheat grain texture variation. hordoindolines, ...	2002	12033628
the voltage-independent cation channel in the plasma membrane of wheat roots is permeable to divalent cations and may be involved in cytosolic ca2+ homeostasis.	a voltage-independent cation (vic) channel has been identified in the plasma membrane of wheat (triticum aestivum) root cells (p.j. white [1999] trends plant sci 4: 245-246). several physiological functions have been proposed for this channel, including roles in cation nutrition, osmotic adjustment, and charge compensation. here, we observe that ca(2+) permeates this vic channel when assayed in artificial, planar lipid bilayers, and, using an energy barrier model to describe cation fluxes, predi ...	2002	12428003
genus-specific localization of the taii family of tandem-repetitive sequences in either the centromeric or subtelomeric regions in triticeae species (poaceae) and its evolution in wheat.	the taii family sequences are classified as tandem repetitive dna sequences present in the genome of tribe triticeae, and are localized in the centromeric regions of common wheat, but in the subtelomeric heterochromatic regions of leymus racemosus and related species. in this study, we investigated the chromosomal distribution of taii family sequences in other triticeae species. the results demonstrated a centromeric localization in genera triticum and aegilops and subtelomeric localization in o ...	2002	12416628
non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase is post-translationally phosphorylated in heterotrophic cells of wheat (triticum aestivum).	in wheat, non-phosphorylating, nadp-dependent glyceraldehyde-3-phosphate dehydrogenase (gapn) was found to be encoded by one gene giving rise to a single protein. however, western blots revealed two different subunits of about 58 and 60 kda in endosperm and shoots. the latter was attributed to in vivo phosphorylation of shoot gapn. no modification occurred in leaves, where the enzyme is composed by a single 58 kda polypeptide. gapn partially purified from shoots and endosperm was dephosphorylate ...	2002	12387887
germination and growth inhibitors from wheat (triticum aestivum l.) husks.	on the basis of our findings that the germination of intact wheat seeds (with husks) belonging to dormancy varieties was restrained as compared with that of the dehusked seeds (grains), the germination inhibitors in the husks were explored. the water-soluble extracts from the husks were separated by the aid of inhibition assay experiments, resulting in the characterization of 2-phenylethyl alcohol 1, 4-vinylphenol 2 and its 2-methoxy derivative 3, and dihydroactinidiolide 4, all of which showed ...	2002	12381108
semi-specific pcr for the evaluation of diversity among cultivars of wheat and triticale.	the usefulness of a semispecific pcr system for the evaluation of genetic diversity among selected cultivars of wheat (triticum aestivum l.) and triticale (x triticosecale wittm.) was investigated. a comparison was made of the diversity among cultivars of both species as revealed by primers targetting the semi-conservative sequences of the intron-exon junction. the main purpose of the study was the selection of the most informative primers from among the exon targetting (et) and intron targettin ...	2002	12378263
the abscisic acid-responsive kinase pkaba1 interacts with a seed-specific abscisic acid response element-binding factor, taabf, and phosphorylates taabf peptide sequences.	the abscisic acid (aba)-induced protein kinase pkaba1 is present in dormant seeds and is a component of the signal transduction pathway leading to aba-suppressed gene expression in cereal grains. we have identified a member of the aba response element-binding factor (abf) family of basic leucine zipper transcription factors from wheat (triticum aestivum) that is specifically bound by pkaba1. this protein (taabf) has highest sequence similarity to the arabidopsis aba response protein abi5. in two ...	2002	12376648
effects of lanthanum and cerium on the vegetable growth of wheat (triticum aestivum l.) seedlings.		2002	12375123
investigation and correction of the gene-derived sequence of glutenin subunit 1dx2 by matrix-assisted laser desorption/ionisation mass spectrometry.	direct matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (maldi-tofms) analysis of a mixture of tryptic peptides was used to verify the gene-derived amino acid sequence of the high molecular weight (hmw) subunit 1dx2 of bread wheat. analysis of the digest was performed by recording several maldi mass spectra of the mixture at low, medium and high mass ranges, and optimising the matrix and the acquisition parameters for each mass range. this resulted in coverage of the ...	2002	12362381
ca(2+)-induced high amplitude swelling and cytochrome c release from wheat (triticum aestivum l.) mitochondria under anoxic stress.	under stress conditions, mitochondria sense metabolic changes, e.g. in ph, cytoplasmic ca(2+), energy status, and reactive oxygen species (ros), and respond by induction of the permeability transition pore (ptp) and by releasing cytochrome c, thus initiating the programmed cell death (pcd) cascade in animal cells. in plant cells, the presence of all the components of the cascade has not yet been shown. in wheat (triticum aestivum l.) root mitochondria, the onset of anoxia caused rapid dissipatio ...	2002	12324275