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residues and contaminants in tea and tea infusions: a review.consumers are very aware of contaminants that could pose potential health hazards. most people drink tea as an infusion (adding hot water); however, in some countries, including india, china and egypt, tea is drunk as a decoction (tea and water are boiled together). an infusion usually brings the soluble ingredients into solution, whereas a decoction brings all soluble and non-soluble constituents together. therefore, a cup of tea may contain various kinds of contaminants. this review focuses on ...201425164107
tea derived galloylated polyphenols cross-link purified gastrointestinal mucins.polyphenols derived from tea are thought to be important for human health. we show using a combination of particle tracking microrheology and small-angle neutron scattering that polyphenols acts as cross-linkers for purified gastrointestinal mucin, derived from the stomach and the duodenum. both naturally derived purified polyphenols, and green and black tea extracts are shown to act as cross-linkers. the main active cross-linking component is found to be the galloylated forms of catechins. the ...201425162539
black hairy tongue syndrome.black hairy tongue (bht) is a benign medical condition characterized by elongated filiform lingual papillae with typical carpet-like appearance of the dorsum of the tongue. its prevalence varies geographically, typically ranging from 0.6% to 11.3%. known predisposing factors include smoking, excessive coffee/black tea consumption, poor oral hygiene, trigeminal neuralgia, general debilitation, xerostomia, and medication use. clinical presentation varies but is typically asymptomatic, although aes ...201425152586
organochlorine pesticides and pyrethroids in chinese tea by screening and confirmatory detection using gc-nci-ms and gc-ms/ms.one hundred and one tea samples including green tea, dark tea, scented tea, black tea, and oolong tea were screened and confirmed for the contamination of 31 organochlorine pesticides (ocps) and 19 pyrethroids (pys) by gas chromatography-negative chemical ionization-mass spectrometry (gc-nci-ms) and gas chromatography-tandem mass spectrometry (gc-ms/ms). 50 pesticides, 3 deuterium-labeled pys, and 24 (13)c-labeled ocps were separated well with the limits of detection (lods) ranging from 0.02 to ...201424964290
effectiveness of green tea mouthwash in comparison to chlorhexidine mouthwash in patients with acute pericoronitis: a randomized clinical trial.the objective of this study was to evaluate the effectiveness of green tea mouthwash in controlling the pain and trismus associated with acute pericoronitis in comparison to chlorhexidine (chx) mouthwash. ninety-seven patients with acute pericoronitis underwent debridement and received 5% green tea mouthwash (study group) or 0.12% chx mouth rinse (control group). pain (visual analogue scale; vas), number of analgesics, maximum mouth opening (mmo), and number of patients with trismus were determi ...201424954134
anti-inflammatory activity and molecular mechanism of oolong tea theasinensin.oolong tea theasinensins are a group of tea polyphenols different from green tea catechins and black tea theaflavins, and they are considered as bioactive compounds in oolong tea. in the present study, based on the properties of theasinensin and information about inflammatory processes, we investigated the anti-inflammatory activity and molecular mechanisms of theasinensin a (tsa) in both cell and animal models. in the cell model, tsa reduced the levels of pro-inflammatory mediators including in ...201424947273
tea consumption and risk of ischemic stroke: a brief review of the literature.stroke is an important cerebrovascular disease which causes chronic disability and death in patients. despite of its high morbidity and mortality, there are limited available effective neuroprotective agents for stroke. in recent years, the research aimed at finding novel neuroprotective agents from natural origins has been intensified. camellia sinensis l. (tea) is the second most consumed beverage worldwide, after water. it is classified into green and white, oolong, black and red, and pu-erh ...201424938891
anti-wrinkle effects of water extracts of teas in hairless mouse.tea flavonoids and polyphenols are well known for their extraordinary antioxidant activity which is considered important for anti-aging processes in animals. this study evaluated the anti-wrinkle effects of three different kinds of tea (camellia sinensis) water extracts (cswes) including green, white, and black teas using a photoaged hairless mouse model. data showed that the cswe-treatment greatly improved skin conditions of mice suffering from uvb-induced photoaging, based on the parameters in ...201425584148
the association of tea consumption and the risk and progression of prostate cancer: a meta-analysis.many studies have focused on the association of tea consumption and the risk and progression of prostate cancer (pca). however, the evidence is inadequate to draw robust conclusions. to shed light on these inconclusive findings, we conducted a meta-analysis. we searched the database of pubmed and web of science for eligible articles. the relevant data were abstracted by two independent reviewers and performed with stata 11.0. 21 studies were included. the pooled outcomes showed that there was a ...201425550896
a systematic review on exposure to toxic and essential elements through black tea consumption in iran: could it be a major risk for human health?tea is the most popular nonalcoholic beverage worldwide. in recent years, some iranian studies have shown the occurrence of toxic elements in fresh or dried tea leaves as well as in brewed tea. the present study aimed to ascertain the health risks associated with exposure to toxic and essential element through black tea consumption in iran by systematically reviewing the accredited articles in the field.201425538829
phytotherapy and nafld--from goals and challenges to clinical practice.non-alcoholic fatty liver disease (nafld) is a global problem and one of the most common liver diseases in the world. various pharmacological and non-pharmacological therapies seem to be non-effective and the patients are often advised not to expect a positive outcome. hence, even in the modern western society many patients reach for traditional herbal products. silymarin, a lipophilic extract derived from milk thistle (silybum marianum) has been used in liver and bile disorders for centuries. s ...201425514914
effect of tea theaflavins and catechins on microvascular function.beneficial effects of flavonoid-rich black and green tea on macrocirculation have been well established. theaflavins are unique to black tea as they are formed from catechins during the enzymatic oxidation of tea leaves. the study was performed to gain more insight into the effects of theaflavins on microcirculation and to compare effects with another important flavonoid class, the green tea derived catechins, which have been reported to improve vascular function. twenty-four healthy subjects we ...201425514559
transfer of ochratoxin a into tea and coffee beverages.ochratoxin a (ota) is nephrotoxic, hepatotoxic, immunotoxic, neurotoxic, reprotoxic, teratogenic, and carcinogenic (group 2b), being characterized by species and sex differences in sensitivity. despite the fact that ota is in some aspects a controversial topic, ota is the most powerful renal carcinogen. the aim of this study was to make a small survey concerning ota content in black tea, fruit tea, and ground roasted coffee, and to assess ota transfer into beverages. ota content was measured usi ...201425525684
probing behavior of empoasca vitis (homoptera: cicadellidae) on resistant and susceptible cultivars of tea plants.feeding activities of the tea green leafhopper, empoasca vitis (gothe) (homoptera: cicadellidae), on resistant and susceptible cultivars of tea plants (camellia sinensis l.) were recorded and analyzed using the direct current electrical penetration graph (epg) system. six distinct epg waveforms characterizing the feeding behavior of the tea green leafhopper, categorized as waveforms a, c, e, s, f, and r, were obtained during the investigation. duration of passive ingestion, possibly of phloem (e ...201425502031
tea (camellia sinensis (l.)): a putative anticancer agent in bladder carcinoma?the leaves of camellia sinensis (l.) are the source of tea, the second most consumed beverage worldwide. tea contains several chemical compounds such as polyphenols (mainly catechins), caffeine, theophylline, l-theanine, among many others. polyphenolic compounds are the main responsible for its significant antioxidant properties and anticarcinogenic potential. bladder cancer is one of the most common types of cancer, and its progression and onset are thought to be controlled by dietary and lifes ...201425495463
biochemical and transcriptome analyses of a novel chlorophyll-deficient chlorina tea plant cultivar.the tea plant (camellia sinensis (l.) o. kuntze) is one of the most economically important woody crops. recently, many leaf color genotypes have been developed during tea plant breeding and have become valuable materials in the processing of green tea. although the physiological characteristics of some leaf color mutants of tea plants have been partially revealed, little is known about the molecular mechanisms leading to the chlorina phenotype in tea plants.201425491435
21-o-angeloyltheasapogenol e3, a novel triterpenoid saponin from the seeds of tea plants, inhibits macrophage-mediated inflammatory responses in a nf-κb-dependent manner.21-o-angeloyltheasapogenol e3 (ats-e3) is a triterpenoid saponin recently isolated from the seeds of the tea tree camellia sinensis (l.) o. kuntze. ats-e3 has several beneficial properties including anti-inflammatory, antidiabetic, antiatherosclerotic, and anticancer effects. unlike other phenolic compounds isolated from tea plants, there are no studies reporting the pharmacological action of ats-e3. in this study, we therefore aimed to explore the cellular and molecular inhibitory activities of ...201425477714
identification and evaluation of reliable reference genes for quantitative real-time pcr analysis in tea plant (camellia sinensis (l.) o. kuntze).reliable reference selection for the accurate quantification of gene expression under various experimental conditions is a crucial step in qrt-pcr normalization. to date, only a few housekeeping genes have been identified and used as reference genes in tea plant. the validity of those reference genes are not clear since their expression stabilities have not been rigorously examined. to identify more appropriate reference genes for qrt-pcr studies on tea plant, we examined the expression stabilit ...201425474086
carboxymethyl- and carboxyl-catechins from ripe pu-er tea.ripe pu-er tea, a special microbial postfermented tea originated from yunnan province, china, since ancient times, is made from green pu-er tea prepared from the leaves of camellia sinensis var. assamica (theaceae). chemical investigation on thearubigin (n-buoh-soluble) fraction of the commercial ripe pu-er tea, led to the identification of four new flavan-3-ol derivatives, 8-carboxymethyl-(+)-catechin (1), 8-carboxymethyl-(+)-catechin methyl ester (2), 6-carboxymethyl-(+)-catechin (3), and 6-ca ...201425455197
metabolite profiling of camellia sinensis by automated sequential, multidimensional gas chromatography/mass spectrometry reveals strong monsoon effects on tea constituents.seasonal variation in tea (camellia sinensis (l.) kuntze; theaceae) chemistry was investigated using automated sequential, multidimensional gas chromatography/mass spectrometry (gc-gc/ms). metabolite libraries were produced for teas harvested from the bulang mountains in yunnan, china before and after the onset of the east asian monsoon. a total of 201 spring and 196 monsoon metabolites were identified, with 169 common and 59 seasonally unique compounds. an additional 163 metabolites were detect ...201425454147
chemoprevention against arsenic-induced mutagenic dna breakage and apoptotic liver damage in rat via antioxidant and sod1 upregulation by green tea (camellia sinensis) which recovers broken dna resulted from arsenic-h2o2 related in vitro oxidant stress.green tea (camellia sinensis; cs) strongly reverses/prevents arsenic-induced apoptotic hepatic degeneration/micronecrosis and mutagenic dna damage in in vitro oxidant stress model and in rat as shown by comet assay and histoarchitecture (he and pas staining) results. earlier, we demonstrated a link between carcinogenesis and impaired antioxidant system-associated mutagenic dna damage in arsenic-exposed human. in this study, arsenic-induced (0.6 ppm/100 g body weight/day for 28 days) impairment o ...201425436473
non-targeted metabolomics study for the analysis of chemical compositions in three types of tea by using gas chromatograph-mass spectrometry and liquid chromatography-mass spectrometry.tea is one of the most widely consumed beverages in the world for its benefits to daily life and health. to discover the difference and correlation of chemical compositions in the three typical types of tea, a non-targeted metabolomics method was developed. after the optimization of extraction methods, gas chromatography-time-of-flight mass spectrometry and liquid chromatography-quadrupole time-of-flight mass spectrometry were applied for metabolomics analysis, 1,812 and 2,608 features were obta ...201425434114
risk factors for renal cell carcinoma in a japanese population.the incidence of renal cell carcinoma (rcc) is high in western and northern europe and north america, and low in asia. although the incidence of rcc in japan is lower than the rates in the other industrialized countries, there is no doubt that it is increasing. in this paper, we would like to introduce the summary of findings of jacc study, which evaluate the risk factors for rcc in a japanese population. jacc study suggests nine risk factors (i.e., smoking, obesity, low physical activity, hyper ...201425422180
investigation of isomeric flavanol structures in black tea thearubigins using ultraperformance liquid chromatography coupled to hybrid quadrupole/ion mobility/time of flight mass spectrometry.ultra performance liquid chromatography (uplc) when coupled to ion mobility (ims)/orthogonal acceleration time of flight mass spectrometry is a suitable technique for analyzing complex mixtures such as the black tea thearubigins. with the aid of this advanced instrumental analysis, we were able to separate and identify different isomeric components in the complex mixture which could previously not be differentiated by a conventional high performance liquid chromatography/tandem mass spectrometry ...201425395124
comparison of the level of boron concentrations in black teas with fruit teas available on the polish market.the determination of boron by inductively coupled plasma-atomic emission spectrometry has been carried in water-soluble and acid soluble (total content) fractions of 36 samples of traditional black tea and fruit brew. the estimation of the impact of the type of tea on the concentration of boron in water-soluble and acid extracts and potential human health risk from the daily intake of boron was carried out in this study. the levels of boron differed significantly in black and fruit tea types. th ...201425379551
assessment of anti-nutritive activity of tannins in tea by-products based on in vitro rumen fermentation.nutritive values of green and black tea by-products and anti-nutritive activity of their tannins were evaluated in an in vitro rumen fermentation using various molecular weights of polyethylene glycols (peg), polyvinyl pyrrolidone (pvp) and polyvinyl polypyrrolidone as tannin-binding agents. significant improvement in gas production by addition of peg4000, 6000 and 20000 and pvp was observed only from black tea by-product, but not from green tea by-product. all tannin binding agents increased nh ...201425358316
using tea as an artificial urine in a canadian performance testing program for fission/activation products.in recent years, the national calibration reference centre for bioassay and in vivo monitoring (ncrc) at the radiation protection bureau (rpb), health canada, has been conducting investigations with black tea to develop a matrix that can be used to replace urine in each of the following performance testing programs (ptp): (1) tritium, (2) carbon-14, (3) the dual (i.e., 3h/14c), and (4) fission/activation products (f/ap). a 1% tea solution with thimerosal, which had worked successfully for tritiu ...201425353238
black tea increased survival of caenorhabditis elegans under stress.the present study examined the effects of black tea (camellia sinensis) extracts (bte) in caenorhabditis elegans under various abiotic stressors. results showed bte increased nematode resistance to osmosis, heat, and uv irradiation treatments. however, bte could not increase nematodes' lifespan under normal culture conditions and mncl2-induced toxicity at concentrations we used. further studies showed that bte decreased reactive oxygen species and up-regulated some antioxidant enzymes, including ...201425345740
calciphytoliths (calcium oxalate crystals) analysis for the identification of decayed tea plants (camellia sinensis l.).the history of tea is poorly known, mainly due to the questionable identification of decayed tea plants in archaeological samples. this paper attempts to test the utility of calciphytoliths (calcium oxalate crystals) for the identification of tea in archaeological samples. it provides the first survey of the macropatterns of calciphytoliths in several species of theaceae and common non-theaceae plants. crystals were extracted from 45 samples of tea, theaceae and common non-theaceae plants, and d ...201425342006
a biochemical study on ameliorative effect of green tea (camellia sinensis) extract against contrast media induced acute kidney injury.reactive oxygen species have been shown to be mediators of kidney injury and green tea polyphenols are potent-free radical scavengers.201425340167
intake of dietary flavonoids and risk of epithelial ovarian cancer.the impact of different dietary flavonoid subclasses on risk of epithelial ovarian cancer is unclear, with limited previous studies that have focused on only a few compounds.201425332332
identification and characterization of cold-responsive micrornas in tea plant (camellia sinensis) and their targets using high-throughput sequencing and degradome analysis.micrornas (mirnas) are approximately 19 ~ 21 nucleotide noncoding rnas produced by dicer-catalyzed excision from stem-loop precursors. many plant mirnas have critical functions in development, nutrient homeostasis, abiotic stress responses, and pathogen responses via interaction with specific target mrnas. camellia sinensis is one of the most important commercial beverage crops in the world. however, mirnas associated with cold stress tolerance in c. sinensis remains unexplored. the use of high- ...201425330732
de novo assembly and transcriptome characterization: novel insights into catechins biosynthesis in camellia sinensis.tea is a popular natural non-alcoholic beverage consumed worldwide due to its bioactive ingredients, particularly catechins (flavan-3-ols). catechins not only contribute to tea quality but also serve important functions in the anti-stress regulation of secondary metabolic pathways. however, the percentages of various catechins are different among tea plant [camellia sinensis (l.) o. kuntze] cultivars. this study aimed to elucidate the biosynthetic mechanism of catechins. transcriptomes from leaf ...201425316555
green tea extract and the risk of drug-induced liver injury.catechins of green tea extract (gte) have been associated with the rare risk of hepatotoxicity in a few individuals. as gte were coadministered with synthetic drugs in some hepatotoxicity cases, uncertainty emerged whether gte are a risk factor of drug-induced liver injury (dili).201425316200
[the effects of lifestyle factors on the incidence of central obesity in chinese adults aged 35-74 years].to understand the incidence of central obesity and its characteristics, and explore the effects of lifestyle factors on incidence of central obesity in chinese adults aged 35-74 years.201425312565
ginseng, green tea or fibrate: valid options for nonalcoholic steatohepatitis prevention?panax ginseng, camellia sinensis and bezafibrate were compared for their lipid-lowering, antioxidant and anti-inflammatory properties as potential agents to prevent nonalcoholic fatty liver disease and its progression to nonalcoholic steatohepatitis.201425296088
survey of pyrrolizidine alkaloids in teas and herbal teas on the swiss market using hplc-ms/ms.pyrrolizidine alkaloids (pas) are a large class of natural compounds amongst which the esterified 1,2-unsaturated necine base is toxic for humans and livestock. in the present study, a method was developed and validated for the screening and quantification of nine pas and one pa n-oxide in teas (camellia sinensis (l.) o. kuntze) and herbal teas (camomile, fennel, linden, mint, rooibos, verbena). samples were analysed by hplc on a rp-column, packed with sub-2 μm core-shell particles, and quantifi ...201425286874
effects of extreme climate events on tea (camellia sinensis) functional quality validate indigenous farmer knowledge and sensory preferences in tropical china.climate change is impacting agro-ecosystems, crops, and farmer livelihoods in communities worldwide. while it is well understood that more frequent and intense climate events in many areas are resulting in a decline in crop yields, the impact on crop quality is less acknowledged, yet it is critical for food systems that benefit both farmers and consumers through high-quality products. this study examines tea (camellia sinensis; theaceae), the world's most widely consumed beverage after water, as ...201425286362
optimization of a dna nicking assay to evaluate oenocarpus bataua and camellia sinensis antioxidant capacity.this study was aimed at assessing the dna damage protective activity of different types of extracts (aqueous, methanolic and acetonic) using an in vitro dna nicking assay. several parameters were optimized using the puc18 plasmid, especially feso4, edta, solvent concentrations and incubation time. special attention has been paid to removing the protective and damaging effect of the solvent and feso4 respectively, as well as to identifying the relevant positive and negative controls. for each sol ...201425302614
analytical strategy coupled with response surface methodology to maximize the extraction of antioxidants from ternary mixtures of green, yellow, and red teas (camellia sinensis var. sinensis).this work aimed at using a simplex-centroid design to model the effects of green, yellow, and red tea mixtures (camellia sinensis var. sinensis) on metal chelation activity, phenolic composition, antioxidant activity, and instrumental taste profile. the regression models that described the extraction of flavan-3-ols, o-diphenols, total phenolic compounds (tpc), free radical scavenging activity toward 2,2-diphenyl-1-picrylhydrazyl radical (dpph), cupric ion reducing antioxidant activity (cuprac), ...201425270398
molecular mechanisms by which white tea prevents oxidative stress.the flavonoid content of tea (camellia sinensis) has beneficial properties in the prevention of diseases. however, the mechanisms by which white tea can protect against oxidative stress remain unclear. to shed light on this issue, rats were given distilled water (controls), 0.15 mg/day (dose 1) or 0.45 mg/day (dose 2) of solid tea extract/kg body weight for 12 months. all the animals received an injection of adriamycin (adr; 10 mg/kg body weight), except half of the control group, which were giv ...201425255956
camellia sinensis (l.) kuntze extract ameliorates chronic ethanol-induced hepatotoxicity in albino rats.the goal of this study was to investigate the hepatoprotective effects of aqueous extract of camellia sinensis or green tea extract (aqgte) in chronic ethanol-induced albino rats. all animals were divided into 4 groups in the study for a 5-week duration. 50% ethanol was given orally to the rats with two doses (5 mg/kg bw and 10 mg/kg bw) of aqgte. ethanol administration caused a significant increase in the levels of plasma and serum enzymatic markers, alanine aminotransferase (alt), aspartate am ...201425254057
the role of phytochemicals as micronutrients in health and disease.intake of dietary phytochemicals has frequently been associated with health benefits. noninfectious diseases including cardiovascular disease (cvd), cancer and diabetes are major causes of death, whereas dementia cases are also increasing to 'epidemic' proportion. this review will focus on recent progress on mechanisms underlying the potential role of dietary phytochemicals in cvd, diabetes, cancer and dementia, with consideration of the latest clinical data.201425252018
the galloyl catechins contributing to main antioxidant capacity of tea made from camellia sinensis in china.total polyphenol content, catechins content, and antioxidant capacities of green, dark, oolong, and black teas made from camellia sinensis in china were evaluated. the total polyphenol content of 20 samples of tea was in the range of 7.82-32.36%. total catechins content was in the range of 4.34-24.27%. the antioxidant capacity of tea extract was determined by the oxygen radical absorbance capacity (orac) test and the 1,1-diphenyl-2-picrylhydrazyl (dpph) radical-scavenging test. total polyphenol ...201425243234
comparison of the inhibition of monoamine oxidase and butyrylcholinesterase activities by infusions from green tea and some citrus peels.this study sought to investigate the effect of infusions from green tea (camellia sinensis) and some citrus peels [shaddock (citrus maxima), grapefruit (citrus paradisi), and orange (citrus sinensis)] on key enzymes relevant to the management of neurodegenerative conditions [monoamine oxidase (mao) and butyrylcholinesterase (bche)]. the total phenol contents and antioxidant activities as typified by their 2,2(')-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (abts) radicals scavenging abiliti ...201425243093
a phase ii clinical trial of a natural formulation containing tea catechins for xerostomia.previous animal studies indicated catechins from the tea plant (camellia sinensis) may modulate salivary function and possess a therapeutic effect for xerostomia. the objective of this study was to evaluate a natural formulation containing tea catechins in 60 patients with xerostomia, including patients with sjögren syndrome.201425240992
effect of black tea consumption on blood cholesterol: a meta-analysis of 15 randomized controlled trials.the results of the studies that have investigated the effects of black tea on blood cholesterol are inconsistent. the aim of this study is to quantitatively assess the effects of black tea on cholesterol concentrations.201425237889
toxicity studies on herbal formulation used in diabetes mellitus.in current study herbal formulation was prepared for diabetes mellitus (type 2). it consists of the extracts of salacia reticulate, cinnamomum zeylanicum,lagerstroemia speciosa, camellia sinensis and gymnema sylvester. toxicitystudies were carried out on heart, liver, kidney and blood of both male and female rabbits. drug was administered in a dose of 15mg/kg body weight daily for 90 days. on 91th day, blood was drawn from animals and investigated for changes in biochemical and hematological lev ...201426045373
can tea consumption be a safe and effective therapy against diabetes mellitus-induced neurodegeneration?diabetes mellitus (dm) is a metabolic disease that is rapidly increasing and has become a major public health problem. type 2 dm (t2dm) is the most common type, accounting for up to 90-95% of the new diagnosed dm cases. the brain is very susceptible to glucose fluctuations and hyperglycemia-induced oxidative stress (os). it is well known that dm and the risk of developing neurodegenerative diseases are associated. tea, camellia sinensis l., is one of the most consumed beverages. it contains seve ...201425977676
the effect of ginkgo biloba and camellia sinensis extracts on psychological state and glycemic control in patients with type 2 diabetes mellitus.interest in finding natural antioxidants for use in food or medical materials to prevent free radical imbalance has increased considerably over the past years. the aim of this research was to evaluate changes in glycemic control and psychological state of patients with type 2 diabetes mellitus (t2dm) after use of antioxidant plant preparations. fifty-six patients with t2dm were randomly allocated to receive standardized ginkgo biloba l. leaves dry extract, green tea dry extract, or placebo capsu ...201425918808
chinese green tea consumption reduces oxidative stress, inflammation and tissues damage in smoke exposed rats.one cause of cigarette smoking is oxidative stress that may alter the cellular antioxidant defense system, induce apoptosis in lung tissue, inflammation and damage in liver, lung, and kidney. it has been shown that chinese green tea (cgt) (lung chen tea) has higher antioxidant property than black tea. in this paper, we will explore the preventive effect of cgt on cigarette smoke-induced oxidative damage, apoptosis and tissues inflammation in albino rat model.201425729541
green tea extracts reduce adipogenesis by decreasing expression of transcription factors c/ebpα and pparγ.this study is to determine if green tea (camellia sinensis) extracts (gte) affects adipogenesis and further investigate the related molecular mechanisms.201425663987
antioxidant capacity of 55 medicinal herbs traditionally used to treat the urinary system: a comparison using a sequential three-solvent extraction process.the prevalence of chronic renal disease exceeds 10% in industrialized societies. oxidative damage is thought to be one of the main mechanisms involved in nearly all chronic renal pathologies.201417309384
optimisation of supercritical carbon dioxide extraction of essential oil of flowers of tea (camellia sinensis l.) plants and its antioxidative activity.to extract natural volatile compounds from tea (camellia sinensis) flowers without thermal degradation and residue of organic solvents, supercritical fluid extraction (sfe) using carbon dioxide was employed to prepare essential oil of tea flowers in the present study. four important parameters--pressure, temperature, static extraction time, and dynamic extraction time--were selected as independent variables in the sfe.201423754602
estimated dietary intakes and sources of flavanols in the german population (german national nutrition survey ii).data from intervention studies suggest a beneficial effect of flavanols on vascular health. however, insufficient data on their intake have delayed the assessment of their health benefits. the aim of this study was to estimate intake of flavanols and their main sources among people living in germany.201423917449
camellia sinensis (green tea) extract attenuate acrylamide induced testicular damage in albino rats.acrylamide is a proved toxin for testicular function, found in food when heated for long period of time. green tea (camellia sinensis) is a potent antioxidant; the aim of this study was to investigate the protective effect of green tea extract against the toxic effects of acrylamide in rat testes.201423364758
camellia sinensis neuroprotective role in experimentally induced hydrocephalus in wistar rats.this study tested possible neuroprotective effects of camellia sinensis-extracted polyphenols in experimental hydrocephalus in young rats.201424005799
tlc bioautographic method for detecting lipase inhibitors.bioautographic assays using tlc play an important role in the search for active compounds from plants. a tlc bioautographic assay has previously been established for the detection of acetylcholinesterase inhibitors but not for lipases.201422095552
bioactive metabolites from the endophytic fungus alternaria alternata.two altenuene derivatives (1-2) and one isocoumarin (3), together with six known compounds (4-9) were isolated from solid cultures of an endophytic fungus alternaria alternata, obtained from the fresh branches of camellia sinensis. chiral analysis revealed the racemic nature of 1 and 2, which were subsequently resolved into two pairs of enantiomers [(+)-1 and (-)-1, (+)-2 and (-)-2]. structures of all the isolates were identified through spectroscopic data. absolute configurations of the two pai ...201425261763
warfarin interactions with medicinal herbs.recognition of the adverse effects of medicinal herbs is not routine and the reports on such effects are even less frequent in clinical practice. potential herb-drug interactions are of a major safety concern, especially for drugs with narrow therapeutic indices like warfarin, which can lead to severe adverse reactions that are sometimes life-threatening. the interactions between warfarin and medicinal herbs described in the literature have been summarized in this paper relying on medline databa ...201425233607
immunity: plants as effective mediators.in the domain of nutrition, exploring the diet-health linkages is major area of research. the outcomes of such interventions led to widespread acceptance of functional and nutraceutical foods; however, augmenting immunity is a major concern of dietary regimens. indeed, the immune system is incredible arrangement of specific organs and cells that enabled humans to carry out defense against undesired responses. its proper functionality is essential to maintain the body homeostasis. array of plants ...201424564587
determination of trace and heavy metals in some commonly used medicinal herbs in ayurveda.traditionally, the herbal drugs are well established for their therapeutic benefits. depending upon their geographical sources sometimes the trace and heavy metals' content may differ, which may lead to severe toxicity. so, the toxicological and safety assessment of these herbal drugs are one of the major issues in recent days. eight different plant species including aloe vera, centella asiatica, calendula officinalis, cucumis sativus, camellia sinensis, clitoria ternatea, piper betel and tagete ...201423222691
slow release of plant volatiles using sol-gel dispensers.the black citrus aphid, also known as the tea aphid, (toxoptera aurantii boyer) attacks economically important crops, including tea (camellia sinensis (l.) o. kuntze). in the current study, silica sol-gel formulations were screened to find one that could carry and release c. sinensis plant volatiles to lure black citrus aphids in a greenhouse. the common plant volatile trans-2-hexen-1-al was used as a model molecule to screen for suitable sol-gel formulations. a znose (electronic sensor technolo ...201426470065
viscosity differences between thickened beverages suitable for elderly patients with dysphagia.dysphagia has been associated with a high risk of undernutrition and aspiration pneumonia in the elderly. it is commonly managed by adding commercial thickening agents to thicken liquids. the rheological behavior of these thickeners in water is known but there is lack of information with regard to other liquids. the aim of this study was to determine the viscosity of 11 beverages after adding commercial thickeners and to compare their viscosity with that of thickened water and also with the refe ...201424842337
investigation of processes in black tea manufacture through model fermentation (oxidation) experiments.flavanol depleted whole fresh green tea leaf powder, as reported in the literature, was used as matrix for a systematic study of the endogenous oxidative enzymatic conversion of selected flavanol combinations to theaflavins and thearubigins. the activity of the two crucial enzymes polyphenol oxidase (ppo) and peroxidase (pod) was controlled individually through addition of h2o2 and/or o2. using the endogenous peroxidase only it was shown that (-)-epicatechin alone did not react with pod. accordi ...201425051300
effect of plant extracts on h2o2-induced inflammatory gene expression in macrophages.arctium lappa (al), camellia sinensis (cs), echinacea angustifolia, eleutherococcus senticosus, panax ginseng (pg), and vaccinium myrtillus (vm) are plants traditionally used in many herbal formulations for the treatment of various conditions. although they are well known and already studied for their anti-inflammatory properties, their effects on h2o2-stimulated macrophages are a novel area of study.201425075197
assessment of bioautography and spot screening of tlc of green tea (camellia) plant extracts as antibacterial and antioxidant agents.this study was carried out as a prerequisite to evaluate the therapeutic potential of camellia varieties. the crude extracts of six different plants of green tea camellia assamica and camellia sinensis were tested against three gram-positive and four gram-negative bacteria using agar disk diffusion method at 50 mg/ml concentration. 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and diphenyl-(2,4,6-trinitrophenyl)iminoazanium free radical scavenging methods were performed to evaluate the ...201425284935
the effects of regular hot black tea consumption on nasal carriage of methicillin-resistant staphylococcus aureus.this study aims to evaluate the possible relationship between oral consumption of hot black tea and methicillin-resistant staphylococcus aureus (mrsa) nasal carriage in a mid-sized town population in central anatolia.201425010803
functional analysis of flavonoid 3',5'-hydroxylase from tea plant (camellia sinensis): critical role in the accumulation of catechins.flavonoid 3',5'-hydroxylase (f3'5'h), an important branch point enzyme in tea plant flavan-3-ol synthesis, belongs to the cyp75a subfamily and catalyzes the conversion of flavones, flavanones, dihydroflavonols and flavonols into 3',4',5'-hydroxylated derivatives. however, whether b-ring hydroxylation occurs at the level of flavanones and/or dihydroflavonols, in vivo remains unknown.201425490984
metabolic engineering of saccharomyces cerevisiae for caffeine and theobromine production.caffeine (1, 3, 7-trimethylxanthine) and theobromine (3, 7-dimethylxanthine) are the major purine alkaloids in plants, e.g., tea (camellia sinensis) and coffee (coffea arabica). caffeine is a major component of coffee and is used widely in food and beverage industries. most of the enzymes involved in the caffeine biosynthetic pathway have been reported previously. here, we demonstrated the biosynthesis of caffeine (0.38 mg/l) by co-expression of coffea arabica xanthosine methyltransferase (caxmt ...201425133732
antioxidant and antibacterial activity of the beverage obtained by fermentation of sweetened lemon balm
(melissa officinalis l.) tea with symbiotic consortium 
of bacteria and yeasts.kombucha is a fermented tea beverage which is traditionally prepared by fermenting sweetened black or green tea (camellia sinensis l.) with symbiotic consortium of bacteria and yeasts (scoby). in this study, lemon balm (melissa officinalis l.) was used as the only nitrogen source for kombucha fermentation. during the seven-day fermentation process, ph value, titratable acidity (ta), total phenolic content, phenolic compounds, and antioxidant activity against hydroxyl (˙oh) and 1,1-diphenyl-2-pic ...201427904315
8-c n-ethyl-2-pyrrolidinone substituted flavan-3-ols as the marker compounds of chinese dark teas formed in the post-fermentation process provide significant antioxidative activity.phytochemical investigation of the aqueous extract of pu-erh tea afforded eight novel 8-c n-ethyl-2-pyrrolidinone substituted flavan-3-ols (puerins i-viii) by (1)h, (13)c, two-dimensional nuclear magnetic resonance (nmr) and high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry (hplc-dad-esi/ms) analysis. comparative chemical analysis of green tea, black tea and chinese dark teas confirmed that these compounds were the marker compounds of ...201424444972
antiradical and tea polyphenol-stabilizing ability of functional fermented soymilk-tea beverage.this study examined the potential of two-step fermentation to preserve tps in functional soy-tea beverage. fermented soymilk-tea (fst) was produced by culturing streptococcus thermophilus, lactobacillus delbrueckii ssp. bulgaricus and bifidobacterium longum in soymilk supplemented with tea extract (te). total phenolic content (tpc) and anti-radical activities were determined for fsts and fermented soymilk (fs). a hplc method was employed to quantify nine major tea phenolics in fst products. tpc ...201424731340
occurrence of glycosidically conjugated 1-phenylethanol and its hydrolase β-primeverosidase in tea (camellia sinensis) flowers.a previous study found that 1-phenylethanol (1pe) was a major endogenous volatile compound in tea (camellia sinensis) flowers and can be transformed to glycosically conjugated 1pe (1pe-gly). however, occurrences of 1pe-gly in plants remain unknown. in this study, four 1pe-glys have been isolated from tea flowers. three of them were determined as (r)-1pe β-d-glucopyranoside ((r)-1pe-glu), (s)-1pe-glu, and (s)-1pe β-primeveroside ((s)-1pe-pri), respectively, on the basis of nmr, ms, lc-ms, and gc- ...201425065942
a stress-responsive late embryogenesis abundant protein 7 (cslea7) of tea [camellia sinensis (l.) o. kuntze] encodes for a chaperone that imparts tolerance to escherichia coli against stresses.the present study characterized cslea7, a group 7 late embryogenesis abundant (lea) gene, from tea [camellia sinensis (l.) o. kuntze]. the gene had an open reading frame of 462 base pairs encoding 153 amino acids with calculated molecular weight of 16.63 kda and an isoelectric point (pi) of 4.93. analysis revealed cslea7 to be an intrinsically ordered protein consisting of nine β-strands and two α-helices. cslea7 expressed ubiquitously in all the tissues analyzed with highest level of transcript ...201425052187
evaluation of the potential nephroprotective and antimicrobial effect of camellia sinensis leaves versus hibiscus sabdariffa (in vivo and in vitro studies).green tea and hibiscus are widely consumed as traditional beverages in yemen and some regional countries. they are relatively cheap and the belief is that they improve health state and cure many diseases. the aim of this study was to evaluate the potential protective and antibacterial activity of these two famous plants in vitro through measuring their antibacterial activity and in vivo through measuring nonenzymatic kidney markers dysfunction after induction of nephrotoxicity by gentamicin. gra ...201424949007
administration of a novel plant extract product via drinking water to post-weaning piglets: effects on performance and gut health.the present study evaluated the effects of a novel plant extract (pe) product (grazixtm) on the performance and gut health of weaned piglets challenged with escherichia coli. the pe was a standardised mixture of green tea leaves (camellia sinensis) and pomegranate fruit (punica granatum) obtained by using the livextract™ process. a total of 144 piglets were weaned at 24 days and allocated to 8 for a 35-day experiment with a 2×2×2 factorial design comparing different treatments (water without pro ...201424739350
medicinal plant extracts can variously modify biofilm formation in escherichia coli.low concentrations of black tea and water extracts from medicinal plants arctostaphylos uva-ursi, vaccinium vitis-idaea, tilia cordata, betula pendula and zea mays stimulated biofilm formation in escherichia coli bw25113 up to three times. similar effect was observed for tannic acid and low concentrations of quercetin. in contrast, the extract from urtica dioica reduced biofilm production. pretreatment with plant extracts variously modified antibiotic effects on specific biofilm formation (sbf). ...201424500005
medicinal plant extracts variously modulate susceptibility of escherichia coli to different antibiotics.antioxidant activity of green and black tea and extracts of medicinal plants and their ability to modulate antibiotic susceptibility in escherichia coli were studied. among a number of extracts tested the maximal capacity to scavenge dpph radicals and chelate iron in chemical tests was found in green and black tea, arctostaphylos uva-ursi and vaccinium vitis-idaea. these extracts contained high level of polyphenols and in aerobic conditions exhibited prooxidant features, producing h2o2 and induc ...201423916388
in vitro evaluation of antagonism of endophytic colletotrichum gloeosporioides against potent fungal pathogens of camellia sinensis.an endophytic fungus isolated from camellia sinensis, assam, northeastern india was identified as colletotrichum gloeosporioides on the basis of morphological characteristics and rdna its analysis. this endophytic fungus was evaluated for growth inhibition against tea pathogens pestalotiopsis theae and colletotrichum camelliae. one isolate of c. gloeosporioides showed strong antagonistic activity against pestalotiopsis theae (64 %) and moderate activity against c. camelliae (37 %). fifty percent ...201424891737
bioconversion of wastewater from sweet potato starch production to paenibacillus polymyxa biofertilizer for tea plants.wastewater from the sweet potato starch industry is a large source of nutrient-rich substrates. we assessed whether this wastewater could be used to produce paenibacillus polymyxa biofertilizer for foliar application to tea trees. using the central composite design methods we experientially determined that the optimal culture conditions for p. polymyxa were ph, 6.5; temperature, 29.0 °c; and incubation time, 16 h. under these conditions, a maximum biomass of 9.7 × 10(9) cfu/ml was achieved. we t ...201424576979
in vitro antibacterial activity of camellia sinensis extract against cariogenic microorganisms.dental caries, a ubiquitous multifactorial infectious disease, is primarily caused by microorganisms like streptococcus mutans and lactobacillus acidophilus. use of antimicrobials is an important strategy to curb cariogenic microorganisms.201425538470
effect of oxidative phytochemicals on nicotine-stressed umnsah/df-1 cell line.nicotine is a parasympathomimetic alkaloid found in the nightshade family of plants (solanaceae) and is a cholinergic drug. it acts directly by stimulating the nicotinic or muscarinic receptors or indirectly by inhibiting cholinesterase, promoting acetylcholine release, or by other mechanisms. 3% of tobacco or one cigarette yields 1 mg of nicotine. as nicotine enters the body, it disturbs the healthy functioning of the body. in this study, we isolated umnsah/df-1 cell line from gallus gallus. fo ...201424860736
spectrophotometric evaluation of dental bleaching under orthodontic bracket in enamel and dentin.aware of the diffusion capacity of bleaching in the dental tissues, many orthodontists are subjecting their patients to dental bleaching during orthodontic treatment for esthetic purposes or to anticipate the exchange of esthetic restorations after the orthodontic treatment. for this purpose specific products have been developed in pre-loaded whitening trays designed to fit over and around brackets and wires, with clinical efficacy proven.201425593650
effect of hydro-alcoholic root extract of plumbago zeylanica l alone and its combination with aqueous leaf extract of camellia sinensis on haloperidol induced parkinsonism in wistar rats.herbal medicines have been used to treat pd in ancient medical systems in asian countries such as india, china, japan and korea based on their own anecdotal or experience-based theories. mucuna pruriens commonly known asvelvet beans, or cow itch, are used in case of spasms associated with parkinsonism.201425206060
antimicrobial polyphenols from small tropical fruits, tea and spice oilseeds.the polyphenolic fractions of fruits: terminalia catappa, carissa carandas, ziziphus nummularia; spice oilseeds: thymol, mustard, fenugreek and poppy seeds; and herb: green and black teas were analyzed for their total phenolics, flavonoids and antimicrobial potential. all fractions from fruits, except anthocyanin of c. carandas, displayed substantial antibacterial activity in accordance to their phenolic contents, the difference in activity being quite significant (p < 0.05), highest for t. cata ...201423703103
determination of polycyclic aromatic hydrocarbons in smoked and non-smoked black teas and tea infusions.this study describes the occurrence of polycyclic aromatic hydrocarbons (pahs) in smoked tea and tea infusions, via the monitoring of benzo(a)anthracene, chrysene, benzo(b)fluoranthene and benzo(a)pyrene (pah4) that have been chosen as indicators for the occurrence of pahs in food by the european food safety agency. the concentrations ranged from 1.2 μg/kg for benzo(b)fluoranthene to 125.0 μg/kg for benzo(a)anthracene in smoked tea leaves, and from 0.6 μg/l for benzo(a)anthracene to 1.2 μg/l for ...201424128549
neurobiological effects of the green tea constituent theanine and its potential role in the treatment of psychiatric and neurodegenerative disorders.theanine (n-ethylglutamic acid), a non-proteinaceous amino acid component of green and black teas, has received growing attention in recent years due to its reported effects on the central nervous system. it readily crosses the blood-brain barrier where it exerts a variety of neurophysiological and pharmacological effects. its most well-documented effect has been its apparent anxiolytic and calming effect due to its up-regulation of inhibitory neurotransmitters and possible modulation of seroton ...201423883567
antioxidant effects of camellia sinensis l. extract in patients with type 2 diabetes.the prevalence of diabetes mellitus (dm) has dramatically increased in the past decade. furthermore, increasing evidence from research shows that oxidative stress (os) plays a crucial role in the pathogenesis of diabetes and in its complications. a search for ways to reduce oxidative damage has become the focus of interest for the majority of scientists. in this study, we determined the radical scavenging activity of single green tea constituents by using an on-line high performance liquid chrom ...201428911467
development of sts and caps markers for variety identification and genetic diversity analysis of tea germplasm in taiwan.tea (camellia sinensis) is an important economic crop in taiwan. particularly, two major commercial types of tea (paochong tea and oolong tea) which are produced in taiwan are famous around the world, and they must be manufactured with specific cultivars. nevertheless, many elite cultivars have been illegally introduced to foreign countries. because of the lower cost, large amount of "taiwan-type tea" are produced and imported to taiwan, causing a dramatic damage in the tea industry. it is very ...201428510923
cloning of a new glutathione peroxidase gene from tea plant (camellia sinensis) and expression analysis under biotic and abiotic stresses.tea plant, camellia sinensis (l.) o. kuntze, a well-known heavy metal hyperaccumulator, possesses a powerful tolerance to heavy metals. the heavy metal stresses lead to reactive oxygen species (ros) production, and high concentration of ros is harmful to plants. the glutathione peroxidase gene has positive function to damage induced by ros. to understand the mechanism of tolerance to deferent stresses in tea plant, a new glutathione peroxidase gene of tea plant was cloned and its expression patt ...201428510910
catechin content and the degree of its galloylation in oolong tea are inversely correlated with cultivation altitude.the taste quality of oolong tea generated from leaves of camellia sinensis l. cultivated in the same mountain area is positively correlated to the cultivation altitude, partly due to the inverse correlation with the astringency of the tea infusion. the astringency of oolong tea mostly results from the presence of polyphenolic compounds, mainly catechins and their derivatives. four catechins, (-)-epicatechin (ec) and (-)-epigallocatechin (egc) together with their gallate derivatives (with relativ ...201428911419
green synthesis of iron nanoparticles by various tea extracts: comparative study of the reactivity.iron nanoparticles (fe nps) are often synthesized using sodium borohydride with aggregation, which is a high cost process and environmentally toxic. to address these issues, fe nps were synthesized using green methods based on tea extracts, including green, oolong and black teas. the best method for degrading malachite green (mg) was fe nps synthesized by green tea extracts because it contains a high concentration of caffeine/polyphenols which act as both reducing and capping agents in the synth ...201424793479
green tea and type 2 diabetes.green tea and coffee consumption have been widely popular worldwide. these beverages contain caffeine to activate the central nervous system by adenosine receptor blockade, and due to the caffeine, addiction or tolerance may occur. in addition to this caffeine effect, green tea and coffee consumption have always been at the center of discussions about human health, disease, and longevity. in particular, green tea catechins are involved in many biological activities such as antioxidation and modu ...201428664072
anti-aging potential of phytoextract loaded-pharmaceutical creams for human skin cell longetivity.the exposure to ultraviolet radiations (uvr) is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ros), leading to aging. the skin can be treated and protected from the injurious effects of ros by using various pharmaceutical formulations, such as cream. cream can be loaded with antioxidants to quench ros leading to photo-protective effects. moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. this review arti ...201526448818
efficacy of tooth bleaching with the prior application of a desensitizing agent.in the present study, we evaluated the efficacy of bleaching on enamel and opposite dentin surfaces using 35% hydrogen peroxide (hp) with the prior application of a desensitizing agent.201524357604
reduced testosterone production in tm3 leydig cells treated with aspalathus linearis (rooibos) or camellia sinensis (tea).flavonoids are major compounds of aspalathus linearis and camellia sinensis. they are classified as endocrine disruptors and some have been shown to inhibit testosterone production. tm3 leydig cell cultures were treated with 250-5000 μg ml(-1) a. linearis (unfermented or fermented rooibos) or camellia sinensis (green or black tea) for 24 h in the absence or presence of 6 miu/200 μl human chorionic gonadotropin (hcg). under nonstimulated conditions, all teas tend to decrease testosterone producti ...201524387279
transcriptomic and phytochemical analysis of the biosynthesis of characteristic constituents in tea (camellia sinensis) compared with oil tea (camellia oleifera).tea plants (camellia sinensis) are used to produce one of the most important beverages worldwide. the nutritional value and healthful properties of tea are closely related to the large amounts of three major characteristic constituents including polyphenols (mainly catechins), theanine and caffeine. although oil tea (camellia oleifera) belongs to the genus camellia, this plant lacks these three characteristic constituents. comparative analysis of tea and oil tea via rna-seq would help uncover th ...201526245644
low caffeine content in novel grafted tea with camellia sinensis as scions and camellia oleifera as stocks.caffeine, a purine alkaloid, is a major secondary metabolite in tea leaves. the demand for low caffeine tea is increasing in recent years, especially for health reasons. we report a novel grafted tea material with low caffeine content. the grafted tea plant had camellia sinensis as scions and c. oleifera as stocks. the content of purine alkaloids was determined in the leaves of one-year-old grafted tea plants by hplc. we also characterized caffeine synthase (cs), a key enzyme involved in caffein ...201526058159
next-generation sequencing reveals differentially amplified tandem repeats as a major genome component of northern europe's oldest camellia japonica.northern europe's oldest and largest camellia japonica growing at the pillnitz castle (germany) for over 200 years is of botanical and cultural importance and is a reference for c. japonica molecular scale analysis. in order to provide a fundament for genome analysis of the genus camellia, we characterize the c. japonica tandem repeat fraction, constituting 12.5 % of the pillnitz camellia's genome. a genomic library of the pillnitz c. japonica was produced and illumina sequenced to generate 36 g ...201526582634
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