Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [k-serotype analyses of vibrio parahaemolyticus isolated in northern taiwan, 1983 through 1993]. | from 1983 through 1993, 786 strains of vibrio parahaemolyticus were collected from food-borne disease outbreaks and sporadic cases of diarrheal illness in northern taiwan, involving 42 k-serotypes. five top leading serotypes were k8 (36.8%), k15 (10.8%), k12 (8.7%), k56 (7.9%) and k63 (4.7%). however, a variation of k-serotypes was found during this study period. from 112 food-borne outbreaks associated with this microorganism, only 54 (48.2%) outbreaks were caused by a single serotype, while 58 ... | 1996 | 10592803 |
| clinical effect of levamisole therapy and its immunological control. | 1996 | 229002 | |
| isolation and characterization of a toxic intracellular protein from vibrio parahaemolyticus. | a toxic factor released from disrupted cells of vibrio parahaemolyticus was partially purified by gel filtration after precipitation with (nh4)2so4 at 40% saturation. the factor, which was a thermostable protein of 63 kda, lysed human erythrocytes at a concentration of 0.15 μg ml(-1). its ld50 by intravenous injection into mice was 6.4 μg. fluid accumulated in suckling mice force-fed with the toxic material (1 to 25 μg). haemolytic activity, which occurred maximall at 37°c and ph 7.0 was enhance ... | 1996 | 24415088 |
| production of new restriction endonuclease vpak111bi recognizing sequence 5'-ggwcc-3' in a haemolysin-less mutant of vibrio parahaemolyticus. | high restriction endonuclease activity was found in a haemolysin-less mutant of the vibrio parahaemolyticus 1743-1 strain. the endonuclease, named vpak11bi, recognized the palindromic pentanucleotide sequence of 5'-ggwcc-3' and cleaved double-stranded dna after the first g, which is exactly the same as the specificity of avaii. the haemolysin-less mutant of v. parahaemolyticus is now available for producing the valuable restriction endonuclease on a commercial scale. | 1997 | 27396891 |
| the kinetics of suppression of proliferation of oxidized murine lymphoid cells by sodium borohydride reduction. | 1997 | 179717 | |
| new frontier for radiology: computed tomography. 40th annual preston m. hickey memorial lecture. | the basic principles of computed tomography (ct) and their application are discussed. the impact of this method on neuroradiologic diagnosis is reviewed, including economic considerations and the effect on nuclear medicine and other diagnostic procedures. the initial experiences of the mallinckrodt institute with whole body scanning are illustrated, and failure possibilities for ct are predicted. | 1997 | 179368 |
| congenital granular-cell tumor of alveolar ridge and tongue. report of two cases. | granular-cell tumors in the oral cavity occur primarily on the tongue (myoblastoma) in adults and on the gum pads (congenital epulis) in infants. because of the usual location and age, these histologically and histochemically similar tumors have been separate clinical entities. these case reports, however, report the concurrence of both clinical entities in the same patient. a 7-day-old female infant had a 1 cm. epulis on the right anterior maxilla and two 1.5 mm. masses on the anterior ventrum ... | 1997 | 168531 |
| malignant pheochromocytoma in childhood: report of a case with familial neurofibromatosis. | a 14-year-old male with familial neurofibromatosis and widely disseminated malignant pheochromocytoma is described. current criteria for the diagnosis and management of this rare malignancy are discussed. | 1997 | 232742 |
| [provisional crowns and bridges]. | 1997 | 3250891 | |
| [characterization of haemolysis of the vibrio parahaemolyticus no.93]. | vibrio parahaemolyticus is a causative bacterium of food poisoning, and the haemolysin produced by this organism has been considered as one of the important virulence factors. in order to understand the pathogenic mechanism of this bacterium, the characteristics of haemolysin from vibrio parahaemolyticus isolated from taiwan were studied. one of the clinical strains, v. parahaemolyticus no.93, presents a weak hemolytic zone on 7% nacl-wagatsuma medium. the dna hybridization results show that v. ... | 1997 | 10592808 |
| [levels of thermostable direct hemolysin production by vibrio parahaemolyticus strains carrying both tdh and trh genes]. | one hundred and twenty-five strains of vibrio parahaemolyticus carrying both the tdh and trh genes were selected from the strains isolated from the travelers with diarrhea by an hybridization test using polynucleotide probes. the levels of tdh produced by these strains and the association between the tdh levels and related characteristics in these strains were analyzed. the tdh level varied greatly from strain to strain, but none of the levels was as high as that of the typical kanagawa phenomen ... | 1997 | 9483883 |
| development and evaluation of a predictive model for the effect of temperature and water activity on the growth rate of vibrio parahaemolyticus. | the growth rates of four strains of vibrio parahaemolyticus were measured and compared in a model broth system. the results for the fastest growing strain, based on 77 combinations of temperature and water activity (aw) using nacl as the humectant, were summarised in the form of a predictive mathematical model. the model, of the square-root type includes a novel term to describe the effects of super-optimal water activity, and can be used to predict generation times for the temperature range (8- ... | 1997 | 9506279 |
| evidence for genetic linkage between the ure and trh genes in vibrio parahaemolyticus. | although v. parahaemolyticus does not generally produce urease, several studies have reported urease-positive v. parahaemolyticus isolates from clinical sources. recently, studies have shown a complete coincidence between the urease-producing phenotype of v. parahaemolyticus strains and the possession of the thermostable direct haemolysin (tdh)-related haemolysin (trh) gene (trh). trh, like tdh, is considered to be an important virulence factor in the pathogenesis of v. parahaemolyticus gastroen ... | 1997 | 9511811 |
| isolation of vibrio and pseudomonas from brown shrimp (penaeus californiensis holmes) intestine. | bacteria of the genera vibrio, pseudomonas and aeromonas were isolated from the intestine of apparently healthy brown shrimp (penaeus californiensis holmes) cultured in a tidal pond. species from these genera of bacteria have been reported as shrimp pathogens and have been involved in human gastrointestinal disorders related to seafood consumption. isolation was done first in marine broth, then in selective media (tcbs, cetrimide and macconkey). the oxidase negative strains were discarded as ins ... | 1997 | 10932719 |
| the recovery of heat-stressed vibrio parahaemolyticus in artificial seawater and artificial seawater supplemented with chitin microcosms. | 1997 | 12501344 | |
| the lons gene regulates swarmer cell differentiation of vibrio parahaemolyticus. | vibrio parahaemolyticus differentiates from a polarly flagellated, short, rod-shaped cell known as the swimmer to the elongated, hyperflagellated, and multinucleated swarmer cell type when it is grown on a surface. the swarmer is adapted to movement over and colonization of surfaces. to understand the signal transduction mechanism by which the bacterium recognizes surfaces and reprograms gene expression, we isolated a new class of mutants defective in surface sensing. these mutants were constitu ... | 1997 | 8981986 |
| primary structure and properties of the na+/glucose symporter (sg1s) of vibrio parahaemolyticus. | previously, we cloned and sequenced a dna fragment from vibrio parahaemolyticus and found four open reading frames (orfs). here, we clearly demonstrate that one of the orfs, orf1, is the gene (sgls) encoding a na+/glucose symporter (sgls). we characterize the na+/glucose symporter produced in escherichia coli mutant (jm1100) cells which lack original glucose transport activity and galactose transport activity. we also show that phlorizin, a potent inhibitor of the sglt1 na+/glucose symporter of ... | 1997 | 9045844 |
| membrane attack induced by hlya, a pore-forming toxin of vibrio cholerae. | determining the activity of purified toxins has generally provided the basis for establishing their role in the host-pathogen relationship. the bacterial genus vibrio produces a number of exotoxins in addition to cholera toxin, including haemolysin a (hlya; vibrio cholerae) and thermostable direct haemolysin (tdh; vibrio parahaemolyticus), both of which possess membrane-targeting cytolytic activity. the action of hlya has been analyzed using protocols previously applied to tdh: lysis and flux ex ... | 1997 | 9051414 |
| coliphage and indigenous phage in mamala bay, oahu, hawaii. | public concern over the discharge of primarily treated sewage by two offshore outfalls in mamala bay, oahu, prompted a multidisciplinary study to determine the impact of such activities on the water quality in the bay and at adjacent recreational beaches. as part of this study, we determined the abundance of coliphage as an indicator of fecal pollution along with total viral direct counts and phages infective for vibrio parahaemoltyicus 16 at stations in mamala bay in four quarterly samplings ov ... | 1997 | 9065272 |
| urea hydrolysis and suppressed production of thermostable direct hemolysin (tdh) by vibrio parahaemolyticus associated with presence of tdh-related hemolysin genes. | a total of 18 strains of v. parahaemolyticus isolated from patients of past food poisoning cases occurring in kanagawa prefecture, japan, were assayed for presence of the thermostable direct hemolysin (tdh) gene and the tdh-related hemolysin (trh) genes (trh 1 and trh 2) with specific reference to their ability to hydrolyze urea and tdh production. a polymerase chain reaction assay revealed that all urea-hydrolyzing strains (9 strains) carried either trh 1 gene or trh 2 gene. the strains carryin ... | 1997 | 9099633 |
| food-borne disease outbreaks due to bacteria in taiwan, 1986 to 1995. | between 1986 and 1995, 852 outbreaks of food-borne disease involving 26,173 cases and 20 deaths were reported in taiwan. about 80% of the outbreaks occurred in the warmer months, i.e., between april and october. of the 852 reported outbreaks, 555 (65%) were caused by bacterial pathogens. the three most common bacteria involved were vibrio parahaemolyticus (35%, 197 of 555 outbreaks), staphylococcus aureus (30%, 169 of 555 outbreaks), and bacillus cereus (18%, 104 of 555 outbreaks). | 1997 | 9114420 |
| arsenate resistance as a possible marker in the differentiation of environmental and clinical isolates of vibrio parahaemolyticus. | strains of vibrio parahaemolyticus were isolated from clinical, marine and freshwater fish of calcutta, west bengal, india. drug and metal resistance characteristics were compared for differentiation of clinical and environmental strains. eighteen out of the twenty environmental isolates were resistant to arsenate, unlike the clinical isolates which were all susceptible. all the thirty-five isolates of v. parahaemolyticus were resistant to ampicillin and streptomycin. | 1997 | 9144909 |
| selective survival of a thermostable direct hemolysin-producing vibrio parahaemolyticus in the alimentary tract of a juvenile estuarine gastropod (clithon retropictus). | juvenile estuarine gastropods (clithon retropictus), maintained in ultraviolet ray-irradiated recirculating artificial seawater with a salinity of 20/1000 at 28 degrees c, preserved thermostable direct hemolysin (tdh)-producing strain d-3 of vibrio parahaemolyticus at a level of 10(4)-10(5) colony forming units per gram (cfu/g) and tdh-non-producing strains n-18 and r-13 at a level of 10(1)-10(2) cfu/g in the alimentary tract for at least 21 days after ingestion. in adults, the numbers of the th ... | 1997 | 9152936 |
| infections after shark attacks in australia. | 1997 | 9154554 | |
| production of monoclonal antibodies to the non-membrane-damaging cytotoxin (nmdcy) purified from vibrio cholerae o26 and distribution of nmdcy among strains of vibrio cholerae and other enteric bacteria determined by monoclonal-polyclonal sandwich enzyme-linked immunosorbent assay. | the distribution of a newly described secretogenic non-membrane-damaging cytotoxin (nmdcy) among strains of vibrio cholerae and other enteric bacteria was determined. to accomplish this, monoclonal antibodies against nmdcy were prepared and a sandwich monoclonal-polyclonal enzyme-linked immunosorbent assay (elisa) was developed. by the sandwich elisa, it was determined that 55.6% of the 412 strains of v. cholerae examined produced nmdcy at varying concentrations while 76, 37.9, and 15.6% of the ... | 1997 | 9009346 |
| [the ribonuclease activity of bacteria as a factor in the persistence of the causative agents of sapronotic infections]. | 1997 | 9341006 | |
| cloning and sequencing of the vibrio parahaemolyticus fur gene. | a ferric uptake regulatory gene (fur) was cloned from vibrio parahaemolyticus wp1 by a polymerase chain reaction-based technique followed by functional complementation of a fur mutation in escherichia coli. a sequence analysis showed that, at the amino acid level, the v. parahaemolyticus fur protein is 81% identical with the fur protein from e. coli and over 90% identical with those of the vibrio species. | 1997 | 9343826 |
| bacterial flavohaemoglobins: a consensus sequence and identification of a discrete enterobacterial group and of further bacterial globins. | the amino acid sequences of haemoglobin-like proteins from the bacteria alcaligenes eutrophus, bacillus subtilis, erwinia chrysanthemi, escherichia coli, vibrio parahaemolyticus, vitreoscilla sp. and the yeast saccharomyces cerevisiae were studied. phylogenies based on distance and parsimony analysis showed that the eubacterial group can be easily distinguished from the other haemoglobin-like proteins. the construction of a consensus bacterial flavohaemoglobin based on the alignment of six bacte ... | 1997 | 9351199 |
| historical overview of key issues in food safety. | foodborne transmission of pathogenic and toxigenic microorganisms has been a recognized hazard for decades. even half a century ago we knew about the dangers of botulism from underprocessed canned foods; staphylococcal poisoning from unrefrigerated cream-filled pastries, sliced ham, meat, and poultry salads; and salmonellosis from infected animal products. despite new protective measures, changes in preservation techniques and failure to follow recognized procedures have created new dangers. mor ... | 1997 | 9366600 |
| the tracheae defective gene encodes a bzip protein that controls tracheal cell movement during drosophila embryogenesis. | the tracheae defective (tdf) gene is required for the formation of the tracheal system during drosophila embryogenesis. it encodes a putative bzip transcription factor (tdf). antibodies directed against tdf detect a nuclear protein in all tracheal cells before invagination and throughout tracheal system morphogenesis. examination of tdf mutants revealed that tdf activity is not necessary for determining tracheal cell identity but for subsequent morphogenetic cell movements. tdf activity is under ... | 1997 | 9384592 |
| [studies on bacillary dysentery cases of overseas travellers--during 1979 to 1995]. | a total of 36,780,440 overseas travellers during 1979-1995 (17 years) were quarantined at osaka and kansai airport quarantine station, 84,777 travellers reported themselves suffer from diarrhoea. stools from 29,587 persons were bacteriologically examined. various enteropathogenic bacteria were isolated from 9,766 (33.0%) patients of the stools examined. isolated species were as follows: plesiomonas shigelloides (3,234 cases); salmonella spp. (2,236 cases); enterotoxgenic escherichia coli (1,621 ... | 1997 | 9391324 |
| mutational analysis of amiloride sensitivity of the nhaa na+/h+ antiporter from vibrio parahaemolyticus. | the activity of the nhaa na+/h+ antiporter of vibrio parahaemolyticus is inhibited by amiloride. we found an amino acid sequence in the nhaa that was identical to a putative amiloride binding domain of the na+/h+ exchanger in mammalian cells. we constructed mutant nhaas that had amino acid substitutions in the putative amiloride binding domain by site-directed mutagenesis. these include v62l (val62 replaced by leu), f63y, f64y, and l65f. most mutant nhaas showed decreased sensitivity for amilori ... | 1997 | 9393731 |
| emergence of a unique o3:k6 clone of vibrio parahaemolyticus in calcutta, india, and isolation of strains from the same clonal group from southeast asian travelers arriving in japan. | active surveillance of vibrio parahaemolyticus infection among hospitalized patients in calcutta, india, was initiated in january 1994. the incidence of cases of v. parahaemolyticus infection suddenly increased in february 1996 and has remained high since then. one hundred thirty-four strains of v. parahaemolyticus isolated from january 1994 to august 1996 were examined for serovar, the presence of the thermostable direct hemolysin gene (tdh) and tdh-related hemolysin genes (trh1 and trh2), prod ... | 1997 | 9399511 |
| outbreak of vibrio parahaemolyticus related to raw oysters in british columbia. | 1997 | 9401155 | |
| direct detection by pcr of escherichia coli o157 and enteropathogens in patients with bloody diarrhea. | direct detection of escherichia coli o157 and foodborne pathogens associated with bloody diarrhea were achieved using polymerase chain reaction (pcr) after the preparation of dna from stool specimens using the microspin technique. pcr was compared with cultivation and toxin production tests with respect to the efficiency of detection of each pathogen; e. coli o157, vibrio parahaemolyticus, salmonella serovar enteritidis and campylobacter jejuni. detection of some or all of the above pathogens in ... | 1997 | 9403509 |
| [bacterial diversity during the cholera epidemic in dakar, senegal (1995-1996)]. | vibrio cholerae o:1, serotype ogawa and biotype el tor (76.1%) was responsible of the outbreak of cholera in dakar, senegal (1995-1996). however, other bacteria were isolated, particularly vibrio cholerae non o:1/non o:139, vibrio fluvialis, vibrio alginolyticus. vibrio parahaemolyticus, salmonella sp.p, shigella sp.p (23.9%). the vibrio cholerae o:1 strains are multiresistant to sulfonamide, cotrimoxazole and chloramphenicol. 97% were also resistant to o/129 compound. fluoroquinolone and 3rd ge ... | 1997 | 9410248 |
| [the drug susceptibility pattern of the presumed etiologic agents of infectious enteritis including verotoxin-producing escherichia coli o-157]. | the drug susceptibility patterns were investigated for verotoxin-producing escherichia coli (vtec) including o-157, salmonella spp., vibrio parahaemolyticus and campylobacter jejuni subsp. jejuni that were obtained in and after july 1996. the results are summarized as follows; 1. we found highly resistant strains of vtec to tetracycline (tc) and ampicillin (abpc). minimum inhibitory concentrations (mic) of some of the drugs against vtec in an aerobic condition were significantly different from t ... | 1997 | 9412873 |
| analysis of functional domains of vibrio parahaemolyticus thermostable direct hemolysin using monoclonal antibodies. | neutralizing monoclonal antibodies (mabs) against vibrio parahaemolyticus thermostable direct hemolysin (tdh) were used in probing the functional domains of this toxin. while pre-incubation of tdh with mab 2a-13c inhibited further binding of tdh to erythrocytes, pre-incubation with another mab 1-24 did not, indicating that mab 1-24 epitope resides in a domain which is not involved in binding of tdh to erythrocytes. on the other hand after binding to erythrocytes, tdh could react with mab 1-24 bu ... | 1997 | 9170273 |
| effect of bile on vibrio parahaemolyticus. | many enteric pathogens are thought to enter a viable but nonculturable state when deprived of nutrients. virulent strains of the enteric pathogen vibrio parahaemolyticus are rarely isolated from their low-nutrient aquatic environments, possibly due to their nonculturability. host factors such as bile may trigger release from dormancy and increase virulence in these strains. in this study, the addition of bile or the bile acid deoxycholic acid to estuarine water-cultured bacteria led to an increa ... | 1997 | 9172358 |
| cross-species induction of luminescence in the quorum-sensing bacterium vibrio harveyi. | different species of bacteria were tested for production of extracellular autoinducer-like activities that could stimulate the expression of the luminescence genes in vibrio harveyi. several species of bacteria, including the pathogens vibrio cholerae and vibrio parahaemolyticus, were found to produce such activities. possible physiological roles for the two v. harveyi detection-response systems and their joint regulation are discussed. | 1997 | 9190823 |
| a mutant cell line resistant to vibrio parahaemolyticus thermostable direct hemolysin (tdh): its potential in identification of putative receptor for tdh. | thermostable direct hemolysin (tdh), a pore-forming toxin produced by vibrio parahaemolyticus, is cytotoxic to rat-1, a fibroblast cell line derived from rat embryo. through mutagenesis of rat-1 with nitrosoguanidine, we established a mutant cell line, mr-t1. mr-t1 was over 200 times more resistant to the cytotoxic activity of tdh than rat-1. tdh increased membrane permeability of rat-1 but not of mr-t1. binding analysis showed that, while being able to bind to rat-1. tdh failed to bind to mr-t1 ... | 1997 | 9197471 |
| [incidence of kanagawa phenomenon-positive and -negative vibrio parahaemolyticus strains isolated from traveller's diarrhea and their relation to tdh and trh genes]. | a total of 1,319 strains of vibrio parahaemolyticus isolated from traveller's diarrhea were analysed for kanagawa phenomenon (kp) with the wagatsuma blood agar test and the results were also compared with those of analyses of tdh and trh genes which encode thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh). the majority of the strains (1,152 strains) counting 87.3% had positive kp, among which 1,049 and 103 strains were only tdh and both tdh and trh-positive ones, respectively. ... | 1997 | 9209122 |
| analysis of the thermostable direct hemolysin (tdh) gene and the tdh-related hemolysin (trh) genes in urease-positive strains of vibrio parahaemolyticus isolated on the west coast of the united states. | urease-positive (ure+) and urease-negative (ure-) strains of vibrio parahaemolyticus isolated from patients on the west coast of the united states between 1979 and 1995 were analyzed for the thermostable direct hemolysin (tdh) gene and the tdh-related hemolysin (trh) genes (trh1 and trh2). the dna colony hybridization method with the polynucleotide probes was used to determine the distribution of the genes. of 60 ure+ strains, 59 strains (98%) had the trh (either trh1 or trh2) gene and 54 strain ... | 1997 | 9230364 |
| putative channel components for the fast-rotating sodium-driven flagellar motor of a marine bacterium. | the polar flagellum of vibrio alginolyticus rotates remarkably fast (up to 1,700 revolutions per second) by using a motor driven by sodium ions. two genes, motx and moty, for the sodium-driven flagellar motor have been identified in marine bacteria, vibrio parahaemolyticus and v. alginolyticus. they have no similarity to the genes for proton-driven motors, mota and motb, whose products constitute a proton channel. motx was proposed to be a component of a sodium channel. here we identified additi ... | 1997 | 9260952 |
| comparative sensitivity of 13 species of pathogenic bacteria to seven chemical germicides. | the relative resistance of diverse human bacterial pathogens to commonly used germicidal agents has not been established. | 1997 | 9276546 |
| invasive phenotype of vibrio parahaemolyticus. | many studies have been done on the virulence factors of vibrio parahaemolyticus, which causes acute gastroenteritis. invasion by this bacterium of culture cells in vitro, however, has not been clearly demonstrated. to assess the invasive ability of v. parahaemolyticus, quantitative studies using antibiotic survival assays were done. of 21 isolates examined, 4 could invade caco-2 cells, a human colon carcinoma-derived cell line. invasion of an isolate, aq4023, was inhibited by cytochalasin d, noc ... | 1997 | 9291344 |
| cloning and nucleotide sequence of the gyrb gene of vibrio parahaemolyticus and its application in detection of this pathogen in shrimp. | because biochemical testing and 16s rrna sequence analysis have proven inadequate for the differentiation of vibrio parahaemolyticus from closely related species, we employed the gyrase b gene (gyrb) as a molecular diagnostic probe. the gyrb genes of v. parahaemolyticus and closely related vibrio alginolyticus were cloned and sequenced. oligonucleotide pcr primers were designed for the amplification of a 285-bp fragment from within gyrb specific for v. parahaemolyticus. these primers recognized ... | 1998 | 9464408 |
| a new na+/h+ antiporter, nhad, of vibrio parahaemolyticus. | a gene encoding an na+/h+ antiporter was cloned from chromosomal dna of vibrio parahaemolyticus, a slightly halophilic bacterium, and expressed in escherichia coli cells. the gene enabled mutant e. coli cells, which were unable to grow in the presence of 10 mm licl (or 0.2 m nacl) because of the lack of major na+(li+)/h+ antiporters, to grow under such conditions. we detected na+/h+ antiport activity due to the gene in membrane vesicles prepared from e. coli cells that harbored the plasmid carry ... | 1998 | 9518619 |
| microbial hazard of salted om el-kholoul (wedge shell donax trunculus). | sixty samples of salted om el-kholoul were collected from different localities in giza, alexandria and ismailia. the samples were examined organoleptically and bacteriological examination of samples were performed for enumeration of aerobic, enterobacteriaceae, staph. aureus, enterococci counts as well as isolation and identification of vibrio parahaemolyticus. the mean counts/gm of aerobes, enterobacteriaceae, staph. aureus, strept. faecalis and strept. faecum were 4.9 x 10(5), 4.8 x 10(4), 1.9 ... | 1998 | 9529997 |
| haemolysin production by environmental isolates of vibrio parahaemolyticus from andamans. | sixty marine water samples were collected from various coastal sites around port blair at different times during august 1996 to july 1997. the specimens were subjected to standard procedure for isolation and identification of v. parahaemolyticus. forty four v. parahaemolyticus isolates were detected from these specimens and all showed clear haemolysis on wagatsuma agar plates. the haemolytic activity was abolished by heating the culture supernatants at 60 degrees c for 10 min and enhanced when p ... | 1998 | 9604541 |
| opar, a homolog of vibrio harveyi luxr, controls opacity of vibrio parahaemolyticus. | vibrio parahaemolyticus is an organism well adapted to communal life on surfaces. when grown on a surface or in a viscous layer, the bacterium induces a large gene system and differentiates to swarmer cells capable of movement over and colonization of surfaces. v. parahaemolyticus displays additional phenotypic versatility manifested as variable colony morphology, switching between translucent and opaque colony types. although not itself luminescent, v. parahaemolyticus produces autoinducer mole ... | 1998 | 9620967 |
| effect of mild acid treatment on the survival, enteropathogenicity, and protein production in vibrio parahaemolyticus. | vibrio parahaemolyticus is an important food-borne enteropathogen that encounters various adverse conditions in its native environment or during infection. effects of mild acid treatment on survival under stress conditions, enteropathogenicity, and protein production in this pathogen were investigated. logarithmically grown cells, at ph 7.5 shifted to ph 5.0 for 30 min, were more resistant to subsequent acid challenge at ph 4.4. a two-phase adaptive procedure (ph 5.8 for 30 min; ph 5.0 for 30 mi ... | 1998 | 9632567 |
| outbreak of vibrio parahaemolyticus infections associated with eating raw oysters--pacific northwest, 1997. | during july-august 1997, the largest reported outbreak in north america of culture-confirmed vibrio parahaemolyticus infections occurred. illness in 209 persons was associated with eating raw oysters harvested from california, oregon, and washington in the united states and from british columbia (bc) in canada; one person died. this report summarizes the investigations of the outbreak, which suggest that elevated water temperatures may have contributed to increased cases of illness and highlight ... | 1998 | 9639367 |
| [wound infection caused by vibrio parahaemolyticus]. | 1998 | 9646572 | |
| [a study on chemical and immunochemical properties and chemotaxonomy of lipopolysaccharides of vibrionaceae, in particular vibrio cholerae and v. parahaemolyticus]. | 1998 | 9654940 | |
| norm, a putative multidrug efflux protein, of vibrio parahaemolyticus and its homolog in escherichia coli. | we found that cells of vibrio parahaemolyticus possess an energy-dependent efflux system for norfloxacin. we cloned a gene for a putative norfloxacin efflux protein from the chromosomal dna of v. parahaemolyticus by using an escherichia coli mutant lacking the major multidrug efflux system acrab as the host and sequenced the gene (norm). cells of e. coli transformed with a plasmid carrying the norm gene showed elevated energy-dependent efflux of norfloxacin. the transformants showed elevated res ... | 1998 | 9661020 |
| from the centers for disease control and prevention. outbreak of vibrio parahaemolyticus infections associated with eating raw oysters--pacific northwest, 1997. | 1998 | 9669774 | |
| [bacteriological and virological studies on the cause of sporadic acute gastroenteritis in tama, tokyo (1991-1996)]. | between may 1991 and december 1996, bacteriological and virological investigations on the fecal samples from patient of infantile diarrhea (155 cases), or infectious gastroenteritis (1,409 cases) diagnosed with 9 medical institutions in tama of tokyo were carried out. of 1,564 samples, 722 (46.2%) were enteropathogen positive cases, and mixed infection was also observed in about 15% of the positive cases. among 13 different kinds of enteropathogens identified, the most prevalent one was pathogen ... | 1998 | 9695470 |
| note: molecular cloning of chitinase genes from vibrio anguillarum and v. parahaemolyticus. | chitinase genes from vibrio anguillarum kv9001 and v. parahaemolyticus atcc17802 were cloned into escherichia coli. open reading frames of chitinase genes from v. anguillarum (vac) and v. parahaemolyticus (vpc) are 1755 bp and 1890 bp, respectively. the deduced amino acid sequences of these genes have 71.6% identity. there are two consensus sequence regions in the vac and vpc proteins. the vac gene was highly prevalent in v. anguillarum, and the dna probe of the vac gene hybridized to v. alginol ... | 1998 | 9717305 |
| close proximity of the tdh, trh and ure genes on the chromosome of vibrio parahaemolyticus. | the distribution and location of the virulence-factor genes of vibrio parahaemolyticus, tdh and trh, and the structural gene of urease, urec, were examined on the genomic dnas of 115 clinical isolates of v. parahaemolyticus. the majority of strains (81%) had two copies of tdh on the chromosome, and no copies of trh or ure. southern hybridization with a tdh probe, after pulsed-field gel electrophoresis of notl-digested genomic dna of each strain revealed only single bands, suggesting that the two ... | 1998 | 9782499 |
| the world's mine oyster. | 1998 | 9787608 | |
| survival of vibrio cholerae 01 strains in shrimp subjected to freezing and boiling. | this research was undertaken to assess the resistance of vibrio cholerae 01 strains inoculated into white shrimp, penaeus schimitti, to heating and freezing treatments. shrimp samples with and without carapace were obtained from sao luis, brazil. microbial analysis revealed the presence of marine vibrios including vibrio alginolyticus, vibrio parahaemolyticus, and other vibrios and aerobic gram-negative and gram-positive bacteria that grew on selective medium, thiosulfate-citrate-bile salt-sucro ... | 1998 | 9798148 |
| detection of tdh and trh genes in a urea-hydrolysing environmental isolate of vibrio parahaemolyticus from the andamans. | co-existence of trh gene and urea-hydrolysing property in one of 44 marine water isolates of vibrio parahaemolyticus correlates strongly with both genotypic and phenotypic characteristics of the bacterium. thus, urease-producing phenotype can be considered a marker of virulence for the production of thermostable direct haemolysin-related haemolysin (trh) (i.e. possession of trh gene). the same isolate also possessed the tdh gene. an environmental isolate possessing all the characteristics of a p ... | 1998 | 9805414 |
| investigation of the 1994-5 ukrainian vibrio cholerae epidemic using molecular methods. | thirty-seven vibrio cholerae and four non-cholera vibrio isolates from ukraine, including strains from the epidemic of 1994-5, were analysed by molecular methods. results from pfge and ribotyping indicated that all ukrainian toxigenic v. cholerae were closely related to each other and to an isolate from a patient from pakistan. a non-toxigenic river water strain obtained during the height of the epidemic was more distantly related to these v. cholerae strains, while the vibrio parahaemolyticus i ... | 1998 | 9747752 |
| filamentous bacteriophages of vibrio parahaemolyticus as a possible clue to genetic transmission. | we have previously reported the isolation and characterization of two filamentous bacteriophages of vibrio parahaemolyticus, designated vf12 and vf33. in this study, to understand the potential of these phages as tools for genetic transmission, we investigated the gene structures of replicative-form (rf) dnas of their genomes and the distribution of these dnas on chromosomal and extrachromosomal dnas. the 7,965-bp nucleotide sequences of vf12 and vf33 were determined. an analysis of the overall ... | 1998 | 9748441 |
| the drosophila pax gene eye gone is required for embryonic salivary duct development. | what are the developmental mechanisms required for conversion of an undifferentiated, two-dimensional field of cells into a patterned, tubular organ? in this report, we describe the contribution of the drosophila pax gene eye gone to the development of the embryonic salivary glands and ducts. eye gone expression in salivary tissues is controlled by several known regulators of salivary fate. after the initial establishment of the salivary primordium by sex combs reduced, fork head excludes eye go ... | 1998 | 9753671 |
| manifestation of the kanagawa phenomenon, the virulence-associated phenotype, of vibrio parahaemolyticus depends on a particular single base change in the promoter of the thermostable direct haemolysin gene. | thermostable direct haemolysin of vibrio parahaemolyticus has been shown to be a major virulence factor. the kanagawa phenomenon (kp), haemolysis induced by this haemolysin on a special blood agar medium, is strongly associated with clinical strains. we have been studying the expressions of various tdh genes encoding this haemolysin to elucidate the significance of the tdh genes possessed by kp-negative strains isolated from patients. we examined the importance of the promoter sequence variation ... | 1998 | 9822816 |
| [factors of persistence and (or) pathogenicity in vibrios and aeromonads belonging to different ecotopes]. | factors of persistence and/or pathogenicity in vibrio parahaemolyticus and aeromonas hydrophila (hemolytic, lipase, lecithin, dnaase, rnaase, antilysozyme, "anti-interferon", anticomplementary activities and capacity for absorbing congo red) were studied. the study revealed the interspecific and subpopulation (hospital and extraorganismal parts of the population) differences in the activity of the manifestation of these factors. strong dependence of the whole complex of persistence and pathogeni ... | 1998 | 9825495 |
| crayfish: a newly recognized vehicle for vibrio infections. | we conducted a 1-year case-control study of sporadic vibrio infections to identify risk factors related to consumption of seafood products in two coastal areas of louisiana and texas. twenty-six persons with sporadic vibrio infections and 77 matched controls were enrolled. multivariate analysis revealed that crayfish (p < 0.025) and raw oysters (p < 0.009) were independently associated with illness. species-specific analysis revealed an association between consumption of cooked crayfish and vibr ... | 1998 | 9825776 |
| cloning and sequencing of major capsid protein (mcp) gene of a vibriophage, kvp20, possibly related to t-even coliphages. | a large, tailed, prolate-headed vibriophage designated kvp20 was isolated from seawater. kvp20 was morphologically very similar to the previously described vibriophage, kvp40 (matsuzaki, s., inoue, t., tanaka, s., 1998. virology, 242, 314-318). however, they showed entirely different host specificities and could easily be differentiated from each other by their patterns of dna restriction fragments. the major capsid protein (mcp) gene of kvp20 encoding the precursor of major capsid protein (pro- ... | 1998 | 9813227 |
| [a study on the distribution and etiology of pathogenic vibrio in the 5 coastal provinces of china]. | in order to understand the distribution and pathogenicity of vibrio in the coastal areas of china a study consisted of 51 water samples and foodstuff from 10 different areas in 5 provinces was conducted microbiologically and etiologically. results showed that 45 pathogenic vibrio were identified, with majority of a. caviae (26.7%), v. alginolyticus (22.2%) and v. parahaemolyticus (11.1%), same kinds of vibrio found in feces from patients with acute infectious diarrhea. prevalence rates of pathog ... | 1998 | 10322692 |
| antibacterial activity of marine-derived fungi. | a total of 227 marine isolates of ubiquitous fungi were cultivated on different media and the secondary metabolite content of the extracts (ethyl acetate/chloroform/methanol 3:2:1) characterized by hplc. the fungi were secured from animals, plants and sediments of venezuelan waters (0-10 m) including mangroves and lagoonal areas. the extracts were tested for antibacterial activity. a total of 7 were active towards vibrio parahaemolyticus and 55 towards staphylococcus aureus, representing 18 diff ... | 1998 | 10409050 |
| staging of myeloma. a preliminary study of staging factors and treatment in different stages. | attempts were made to find prognostic factors in myeloma. in 16 deceased patients, urinary light chains, skeletal lesions, and the quantity of the monoclonal protein fraction in the serum were correlated to prognosis, in contrast to the electrophoretic mobility of the monoclonal fraction, the hemoglobin, the serum creatinine value, the serum calcium, or the intestinal calcium absorption. skeletal calcium uptake was only numerically higher in mild myeloma than in advanced myeloma. since these fin ... | 1998 | 106448 |
| the role of magnesium in protein-energy malnutrition. | the present study was aiming to explore further the variations in the levels of mg in both serum and csf of pem children, hoping a better understanding revealed decreased values of both blood serum and csf levels of mg in pem children which denote some abnormalities in brain metabolism. such abnormalities add further support for assumption of the link between dietary insufficiency and subnormal mental performance in pem. | 1998 | 98923 |
| the mental health of nuclear submariners in the united states navy. | 1998 | 107489 | |
| the experience of screening mammography. | nearly 1000 women who had just undergone screening mammography responded to a survey regarding demographics, the circumstances of the mammographic examination, and their responses to it. several findings of interest to the family physician include the following: (1) the majority of respondents obtained the examination as a result of their physician's referral rather than on their own. (2) most respondents experienced less pain during the procedure than they had anticipated. (3) the major express ... | 1998 | 2809522 |
| [colo-rectal carcinomas: a challenge]. | 1998 | 108581 | |
| retrospect and prospect of electron microscopy in japan: biology/virology. | 1998 | 2809471 | |
| [the cranio-carpo-tarsal dysplasia syndrome in a 6-year-old girl (whistling-face syndrome)]. | 1998 | 106974 | |
| cell-number and cell size. | 1998 | 90908 | |
| immunocytochemical characterization of the lymphocytes in nodular lymphoid hyperplasia of the bowel. | the immunoglobulin constituents of hypertrophied lymphoid nodules in the intestinal tracts of six patients with the variable immunodeficiency syndrome and one patient with selective iga deficiency were evaluated by the peroxidase-labeled antibody technique. the nodules were found to contain a dense population of igm-bearing lymphocytes and much intercellular igm. evidence that the cells were engaged in igm synthesis was the presence of the immunoglobulin in the perinuclear spaces and endoplasmic ... | 1998 | 105208 |
| gray scale sonography of cystadenoma of the pancreas: report of two cases. | 1998 | 108314 | |
| catheter cervical vertebral venography. | cervical vertebral venography using catheterzation via the femoral vein was carried out in 78 cases. in disc disease the internal vertebral venous plexus (ivvp) are interrupted at single or multiple levels, and sometimes completely blocked. in ossification of posterior longitudinal ligament, the ivvp are separated bilaterally and interrupted at multiple levels due to centrally situated ossification and associated osteophytes. venography helps to determine the upper and lower limits of laminectom ... | 1998 | 106325 |
| the management of acute genitourinary infections. | 1998 | 124723 | |
| [heredity in eye diseases. ii. foundations of ophthalmological genetics (author's transl)]. | 1998 | 108459 | |
| [continuous measuring of intracranial pressure (icp) via epidural pressure transducer (author's transl)]. | continuous epidural monitoring of icp was performed on 20 patients during a 6 day's period with the model 77450 of philips. a significant decrease of intracerebral pressure after application of sorbitol and dexamethasone could be demonstrated. comparing the very low rate of complications with the resulting advantages, the described method can be recommended for controlled treatment of elevated icp. | 1998 | 107014 |
| a vest for ambulatory patients receiving hyperalimentation. | there have been no significant complications related to the use of this ambulatory hyperalimentation vest. the potential positive impact that increased use of this vest at home might have upon hospital stay and cost containment is obvious. it is further anticipated that, as advances are made in the technology of plastic bags, tubing, miniature pumps and microprocessors, ambulatory or home hyperalimentation delivery systems exemplified by this vest will achieve a much higher degree of sophisticat ... | 1998 | 107602 |
| the new peptide hormones of the gut and their clinical significance. | 1998 | 34965 | |
| fetal heart rate during a maternal grand mal epileptic seizure. | although maternal ingestion of antiepileptic drugs is strongly suspected of causing congenital defects, particularly oral clefts, the effect of epilepsy itself or a combined effect of drug intake and epilepsy have not been excluded as etiological factors. very little is known about fetal oxygenation during a maternal grand mal epileptic seizure. we describe two cases in which fetal heart rate was recorded during a maternal epileptic seizure during labor. the first fetus became clearly asphyctic ... | 1998 | 106102 |
| radiographic changes of the skull in sickle cell anemia. | skull radiographs of 194 patients from 4 months to 55 years old with sickle cell anemia revealed porous decreased bone density in 25%, widening of diploë associated with a relative decrease in the width of the outer table in 22%, and vertical "hair-on-end" striations in 5%. the youngest patient with vertical striations was 5 years old and the oldest was 39. serial examinations in 60 patients revealed no decrease of the skull width nor disappearance of the striations with age. | 1998 | 106639 |
| pole cells of drosophila melanogaster in culture. normal metabolism, ultrastructure, and functional capabilities. | 1998 | 108161 | |
| [hemodialysis and nursing. 2. indication for hemodialysis]. | 1998 | 105196 | |
| prophylactic antibiotics in surgery. practices within surgical services of the veterans administration. | a survey of prophylactic antibiotic-prescribing habits among chiefs of surgical services of the veterans administration yielded a 99% response. these responses have been compared with guidelines set by an expert committee on antimicrobial use for the va central office. assuming that 100% concordance with the standards of the committee is a desired state, the average hospital is approximately one third away from this goal (average score, 62.4%). the majority of errors were those of overuse rather ... | 1998 | 105162 |
| increased insulin sensitivity and cellular insulin binding in obese diabetics following treatment with glibenclamide. | the aim of the present study was to examine the effect of glibenclamide on the insulin receptors, the insulin sensitivity and the insulin secretion in obese non-ketotic diabetics. two groups of 9 obese diabetics were studied before and after 10 days' treatment with a 1200 kcal's diet and a 1200 kcal's diet + 10 mg/day of glibenclamide, respectively. in the group treated with diet alone we found no significant alteration of the insulin secretion pattern (p greater than 0.1). however, the insulin ... | 1998 | 106617 |
| [recrystallization and reaction of urates in gout tophus after fixation with glutaraldehyde]. | uric acid and urates usually dissolve in the instance of routine fixation of material with formol. bioptic confirmation of diagnosis is therefore usually very difficult if a murexide reaction test cannot be carried out concurrently. after fixation in glutaraldehyde for electron optic aims the crystals remain present in microscopic paraffin preparations for a long period of time and undergo even recrystallization which largely facilitates the microscopic diagnosis. it is assumed that a compound o ... | 1998 | 98928 |
| a preliminary assessment of possible mutagenicity of betel nut and ingredients of the betel quid when administered alone or in combinations to larvae of drosophila melanogaster. | the "carcinogenic" betel nut and constituents of betel quid were tested for possible mutagenicity in drosophila. the test compounds were administered either alone or in combinations by larval feeding. the data on sex-chromosome loss, sex-linked recessive lethals and autosomal translocations suggest lack of mutagenicity. | 1998 | 108597 |
| first year radiology residents: pgi vs. pgii. | 1998 | 107768 | |
| cerebrospinal fluid-, blood-, and ascitis-catecholamines in hepatic cirrhosis. | the consideration of the extensive innervation of liver parenchyma, together with data relating to the degradation of catecholamines in the liver and the importance of hepatic vascular process in hepatic cirrhosis, prompted us to investigate the behaviour of catecholamines in this condition. the following parameters were measured in cerebrospinal fluid (csf), blood and ascitis fluid: total catecholamines, adrenaline and noradrenaline. during hepatic cirrhosis, total catecholamines level in blood ... | 1998 | 106413 |
| [stimulation with automatic radiofrequency scanning in the long term treatment of tachyarrythmias]. | a pacemaker is described which uses radiofrequency signals for synchronisation and pacing, and seeks automatically the phase of the cycle at which the tachycardia may be interrupted. the patient himself can activate the pacemaker. the programme of the external transmitter, which scans nearly all the r-r cycles of the tachycardia, may be pre-set to transmit one or two synchronised stimuli, with a progressive delay of 5 or 10 ms. if the tachycardia is interrupted the pacing stops automatically. th ... | 1998 | 105682 |
| acquired red cell pyruvate kinase deficiency in acute leukemias. | 1998 | 107074 |