Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| prostanoids and adrenaline release: a study of [3h]adrenaline efflux from the rabbit isolated, perfused, adrenal gland. | [3h]adrenaline was incorporated in an isolated perfused preparation of the rabbit adrenal gland and the effects of indomethacin, pge2 and pgi2 on its release were investigated. efflux of [3h]adrenaline was elicited by electrical stimulation of the splanchnic nerve (60 s at 5 hz). indomethacin (3 and 30 microm) had no effect on stimulation-induced efflux. pge2 (30, 90 and 300 nm) reduced the efflux; with 90 nm pge2 the inhibition amounted to approximately 30%. pgi2, in concentrations from 90 to 6 ... | 1985 | 2858566 |
| mechanism of tachycardia caused by intracarotid pge2 in conscious ewes. | conscious adult ewes prepared with nonocclusive indwelling vascular catheters were used to determine the mechanism by which heart rate increases during central administration of prostaglandin e2 (pge2). heart rate increased 14 bpm during steady-state intracarotid infusion of pge2, 10 ng/kg/min (p less than 0.05). intravenous atropine methyl bromide, 1 mg/kg, increased heart rate 26 bpm (p less than 0.05) 5 min after injection. heart rate remained elevated 30 min after injection. the heart rate r ... | 1985 | 2864696 |
| trials with portable screen rooms modified for use as animal-baited net traps for mosquito collection. | trials in larimer county, colorado during july and august 1984, with recreational screen rooms modified as large animal-baited mosquito traps are described. the two units tested are free-standing, portable and require no external support. in all-night trials, 462.5 mosquitoes/trap night were captured with horse bait compared with 367/trap night with cdc light traps. in 2-hour evening comparisons, mosquitoes collected per trapping period totalled 416 for horse-bait traps, 132 for light traps, and ... | 1985 | 2906667 |
| [comparisons of vasoconstrictions induced by alpha agonists either in the luminal perfusate or in the adventitial superfusate]. | 1985 | 2869558 | |
| thyroxine induced transformation in sarcoplasmic reticulum of rabbit soleus and psoas muscles. | the properties of the sarcoplasmic reticulum membranes isolated from slow-twitch type i soleus and fast-twitch type ii psoas muscles of control and thyroxine treated rabbits were comparatively studied. membrane yield, maximal calcium storing capacity, atp-supported calcium uptake, calcium-dependent atpase activity and calcium-dependent phosphoprotein formation were found to be 3-10 fold higher in psoas than in soleus preparations. membrane yield, calcium-dependent atpase activity, atp-supported ... | 1985 | 2934902 |
| [influence of pyronaridine phosphate on three-generation reproduction in rats]. | 1985 | 2934948 | |
| [results of a trial of a plague monoclonal antibody erythrocyte diagnostic agent]. | plague antibody monoclonal erythrocyte diagnosticum was studied in serological tests simultaneously with commercial plague antibody erythrocyte diagnosticum prepared on the basis of hyperimmune horse serum and with commercial plague antigenic erythrocyte diagnosticum. in this investigation the suspensions of numerous strains of yersinia pestis, other closely related and heterologous organisms, experimentally infected wild and laboratory animals, as well as samples of materials obtained from smal ... | 1985 | 2936047 |
| cosmid cloning of rickettsia prowazekii antigens in escherichia coli k-12. | rickettsia prowazekii dna was partially digested with sau3a or hindiii, ligated with the cosmid vector phc79, packaged in vitro, and transduced into escherichia coli hb101. cosmid cloning of sau3a-digested rickettsial dna yielded 1,288 ampicillin-resistant colonies; 798 cosmid clones resulted with hindiii-digested rickettsial dna. chimeric cosmid dna was extracted from the latter gene bank, digested to completion with hindiii, and compared by agarose gel electrophoresis with a hindiii digest of ... | 1985 | 2981194 |
| uridine 5'-diphosphate glucose analogues. inhibitors of protein glycosylation that show antiviral activity. | a series of analogues of uridine 5'-diphosphate glucose and uridine 5'-diphosphate glucosamine have been synthesized by reaction of 2,3,4,6-tetra-o-benzyl-, 2,3,4,6-tetra-o-benzoyl-, 2,3,4,6-tetra-o-acetyl-, and 2,3,4,6-tetra-o-palmitoyl-alpha-d-glucopyranose and 2-acetamido-3,4,6-tri-o-acetyl-2-deoxy-alpha-d-glucopyranose with chlorosulfonyl isocyanate and 2',3'-o-isopropylideneuridine. isopropylidene and acetyl groups of the resulting 5'-o-[[[[(alpha-d-glucopyranosyl)oxy]carbonyl]amino]sulfony ... | 1985 | 2981323 |
| middle tumor antigen of polyomavirus transformation-defective mutant ng59 is associated with pp60c-src. | we have found that lysis of mouse embryo cells infected with the polyomavirus host range transformation-defective (hr-t) mutant ng59 under gentle conditions that avoid ionic detergents results in detectable ng59-encoded middle tumor antigen (mtag) associated with pp60c-src. this mtag-pp60c-src complex could be immunoprecipitated from ng59-infected cell lysates by either sera from animals bearing polyomavirus-induced tumors or by monoclonal antibodies directed against mtag. immune complex kinase ... | 1985 | 2981329 |
| respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mrna, identification of cleavage activation site and amino acid sequence of n-terminus of f1 subunit. | the amino acid sequence of respiratory syncytial virus fusion protein (fo) was deduced from the sequence of a partial cdna clone of mrna and from the 5' mrna sequence obtained by primer extension and dideoxysequencing. the encoded protein of 574 amino acids is extremely hydrophobic and has a molecular weight of 63371 daltons. the site of proteolytic cleavage within this protein was accurately mapped by determining a partial amino acid sequence of the n-terminus of the larger subunit (f1) purifie ... | 1985 | 2987829 |
| effect of acute acrylonitrile exposure on metrazol induced seizures in the rat. | the effects of acute exposure to acrylonitrile (acn), 10, 20, or 40 mg/kg by gavage, on the ability of metrazol (mtz) to induce seizures was studied in adult, male sprague-dawley rats. the frequency of seizure occurrence and the frequency of a lethal seizure was greater when the high acn dosage was given in combination with metrazol. this dosage of acn was not lethal when given alone. examination of brain tissue in these animals revealed no difference in cyanide levels when mtz was combined with ... | 1985 | 2986062 |
| pulsed electromagnetic fields promote collagen production in bone marrow fibroblasts via athermal mechanisms. | primary and passaged cultures of fibroblasts (rbmfs) raised from the bone marrow stroma of young rabbits were treated with pulsed electromagnetic fields (pemfs) from the start of each culture until 1 week after they became confluent. the pemf treatment had no effect on cell proliferation, estimated by phase contrast microscopy, by 3h-thymidine incorporation into dna, or by total dna assay. collagen production, estimated by conversion of 3h-proline to 3h-hydroxyproline in nondialyzable material w ... | 1985 | 2988718 |
| effect of acute acrylonitrile exposure on metrazol induced seizures in the rat. | the effects of acute exposure to acrylonitrile (acn), 10, 20, or 40 mg/kg by gavage, on the ability of metrazol (mtz) to induce seizures was studied in adult, male sprague-dawley rats. the frequency of seizure occurrence and the frequency of a lethal seizure was greater when the high acn dosage was given in combination with metrazol. this dosage of acn was not lethal when given alone. examination of brain tissue in these animals revealed no difference in cyanide levels when mtz was combined with ... | 1985 | 2986062 |
| adrenergic receptors: possible mechanism of inverse regulation of alpha- and beta-receptors. | many physiologic and pathologic conditions, including bronchial asthma, are associated with inverse changes in alpha- and beta-receptor-mediated responses in various tissues. the direction of the change elicited by a given stimulus is tissue specific, as exemplified by the actions of thyroid hormones: in the rat heart, hypothyroidism reduces beta- and increases alpha-receptor responses, whereas in the rat liver it has the opposite effects. a similar increase in beta- and decrease in alpha-recept ... | 1985 | 2991359 |
| studies on the atp-sensitivity of yeast phosphofructokinase by means of affinity partitioning using polymer bound cibacron blue f3g-a. | yeast phosphofructokinase was partitioned in an aqueous two-phase system composed of polyethylene glycol and dextran in which a small amount of the first polymer was replaced by cibacron blue f3g-a substituted polyethylene glycol. it was found that the partition coefficient, k, of the enzyme determined immediately after adding the enzyme to the system and within a series of time intervals (min) shifts from a lower to a higher value. this effect was amplified when the enzyme was preincubated with ... | 1985 | 2935143 |
| electron microscopic assessment of cervical punch biopsies in women followed-up for human papillomavirus (hpv) lesions. | cervical punch biopsies from 102 women prospectively followed-up for hpv (human papillomavirus) lesions (flat, inverted and papillomatous condylomas) were assessed on transmission electron microscopy (tem) for the presence of hpv particles. the virus discovery rate was correlated with the other characteristics of significance to the clinical behaviour (natural history) of these lesions, i.e. the colposcopic pattern, expression of papillomavirus genus specific structural antigens (demonstrated by ... | 1985 | 2988479 |
| rates of cholesterol synthesis and low-density lipoprotein uptake in the adrenal glands of the rat, hamster and rabbit in vivo. | the absolute rate of cholesterol acquisition from de novo synthesis and from receptor-dependent and receptor-independent low-density lipoprotein (ldl) uptake was determined in the adrenal glands of the rat, hamster and rabbit under in vivo conditions. the rate of incorporation of [3h]water into cholesterol in the adrenal gland was much higher in the hamster (1727 nmol/h per g) and rabbit (853 nmol/h per g) than in the rat (71 nmol/h per g). assuming that 23 atoms of 3h are incorporated into the ... | 1985 | 2992599 |
| [emergency cardiac pacing via a coronary vessel during percutaneous coronary angioplasty]. | percutaneous coronary angioplasty (pca) is sometimes complicated by bradyarrhythmias necessitating emergency temporary cardiac pacing. this is usually performed by the classical transvenous endocardial approach. this experimental study investigated the possibility of using the metallic guide wire used during pca as a monopolar electrode. systematic electrical stimulation at different levels of the coronary arteries in 6 anaesthetised pigs showed threshold levels in the distal segments of 3 to 15 ... | 1985 | 2937382 |
| [effect of tunicamycin on human cytomegalovirus replication]. | 1985 | 2998078 | |
| [effect of levamisole on the course of chronic cytomegalovirus infection in women]. | the therapeutic effect of orally given levamisole in the treatment of women with chronic cytomegalovirus infection was studied. the patients were examined serologically, cytologically, virologically; the factors of cell-mediated immunity were also studied. oral therapy with levamisole resulted in a definite increase of the cell-mediated immunity with a trend for decrease in antibody titres and reduced virus excretion. evaluation of the clinical effect of levamisole therapy, however, revealed no ... | 1985 | 2998093 |
| [interaction of yersinia pestis bacteria with bacteriophage mu]. | yersinia pestis cells are shown to be sensitive to bacteriophage mu cts62 infection. lysis of bacteria has been shown to be more efficient on solid nutrient medium than in lb broth. 10(-5) pfu per ml is the maximal concentration of bacteriophage particles yielded from the broth cultures of bacteria. moi 0.1 has been used to obtain such yields of bacteriophage. lysogenization of yersinia pestis cells has not been achieved when the standard methods of bacteriophage infection have been used. it was ... | 1985 | 2948125 |
| pyrimidinones. 1. 2-amino-5-halo-6-aryl-4(3h)-pyrimidinones. interferon-inducing antiviral agents. | interferon induction and antiviral activity was discovered with 2-amino-5-bromo-6-phenyl-4(3h)-pyrimidinone. an analogue study incorporating a series of 2-amino-5-substituted-6-arylpyrimidinones revealed that the most potent interferon inducers were mono- and difluorophenyl analogues. these same analogues were also potent antiviral agents against semliki forest virus and herpes simplex type 1. in addition the monomethoxyphenyl analogues were potent antiviral agents but weak interferon inducers. ... | 1985 | 2999405 |
| relationship of acth1-39-immunostained fibers and magnocellular neurons in the paraventricular nucleus of rat hypothalamus. | dual antigen immunocytochemical staining procedures were used in the same tissue section to determine the distribution of acth immunostained fibers and varicosities within the magnocellular and parvocellular divisions in the paraventricular nucleus (pvn) of rat hypothalamus and elucidate its anatomical relationship to vasopressin (vp) and oxytocin (oxy)-containing neurons. double immunostained preparations using glucose oxidase-antiglucose oxidase complex combined with pap complex to visualize t ... | 1985 | 3001668 |
| [the afferent pathway of the hippocampus on participation in acupuncture analgesia. a study of retrograde transport method]. | 1985 | 3004787 | |
| [forestomach carcinoma and hepatoma induced in mice and rats by n-3-methylbutyl-n-1-methylacetonylnitrosamine (mambna)]. | mambna is a new n-nitroso compound isolated from fusarium moniliforme-inoculated corn meal. in the present studies the carcinogenicity of mambna is shown by the induction of forestomach carcinomas and liver tumors in mice and rats following the gastric intubation of this compound. among 42 mice of kunming (km) stock treated with mambna (10-20 mg/week), 22 showed epithelial hyperplasia of the esophagus, 29 papillomas and 4 squamous carcinomas in the forestomach, and there were 6 liver adenomas an ... | 1985 | 3004854 |
| viremia is present in incubation period in nonimmunocompromised children with varicella. | 1985 | 2981309 | |
| corticotropin-releasing factor desensitization of adrenocorticotropic hormone release is augmented by arginine vasopressin. | the desensitization of corticotropin-releasing factor (crf)-stimulated acth release from and intracellular cyclic amp accumulation in anterior pituitary cells was investigated in primary cultures of rat pituitary cells and in a mouse tumor cell line (att 20/d16-16). crf potently stimulated acth secretion and cyclic amp accumulation in both preparations. when primary cultures were preincubated with 100 nm crf, 50% desensitization of acth release occurred within 4 hr while similar desensitization ... | 1985 | 2981299 |
| specific binding of radioiodinated granulocyte-macrophage colony-stimulating factor to hemopoietic cells. | the hemopoietic growth factor granulocyte-macrophage colony-stimulating factor, gm-csf, specifically controls the production of granulocytes and macrophages. this report describes the binding of biologically-active 125i-labeled murine gm-csf to a range of hemopoietic cells. specific binding was restricted to murine cells and neither rat nor human bone marrow cells appeared to have surface receptors for 125i-labeled gm-csf. 125i-labeled gm-csf only appeared to bind specifically to cells in the my ... | 1985 | 2990915 |
| the ultrastructural basis for synaptic transmission between primary muscle afferents and neurons in clarke's column of the cat. | the synaptic connection between primary muscle afferents and dorsal spinocerebellar tract (dsct) neurons has been studied in an attempt to reveal some of the mechanisms underlying excitatory transmission in the mammalian central nervous system. previous electrophysiological experiments have shown that the excitatory postsynaptic potentials (epsps) evoked dsct neurons by impulses in a single muscle afferent fluctuate in amplitude. these fluctuations occur between discrete amplitudes which are sep ... | 1985 | 2991483 |
| diverse involvements of ni protein in superoxide anion production in polymorphonuclear leukocytes depending on the type of membrane stimulants. | effects of islet-activating protein (iap) were examined to assess the involvement of the guanine nucleotide-binding regulatory protein responsible for inhibition of adenylate cyclase system (ni protein) in the superoxide anion (o-2) production in polymorphonuclear leukocytes (pmnl) stimulated with various agents. n-formyl-methionyl-leucyl-phenylalanine (fmlp)-stimulated o-2 production was inhibited by the pretreatment with iap. o-2 production induced by each of phorbol myristate acetate, concana ... | 1985 | 2992509 |
| delay in platelet recovery after bone marrow transplantation: impact of cytomegalovirus infection. | the effect of cytomegalovirus (cmv) infection on hematopoietic recovery after marrow-ablative chemoradiotherapy followed by autologous bone marrow transplantation (bmt) was studied in patients with non-hodgkin's lymphoma of high-grade malignancy and in patients with acute leukemia. the recovery of platelets after autologous bmt occurred significantly quicker in cmv-negative patients than in cmv-positive patients (platelets greater than 50,000 per cubic millimeter after 21 1/2 v 40 days, respecti ... | 1985 | 2994781 |
| results of investigations of the cell surface membranes composition of cells transformed by td-mutant of rsv. | 1985 | 2995158 | |
| insertion of dna sequences into the human chromosomal beta-globin locus by homologous recombination. | a 'rescuable' plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmid integrated into the human beta-globin locus. the planned modification was achieved in about one per thousand transformed cells whether or not the target gene was expressed. | 1985 | 2995814 |
| the ultrastructural basis for synaptic transmission between primary muscle afferents and neurons in clarke's column of the cat. | the synaptic connection between primary muscle afferents and dorsal spinocerebellar tract (dsct) neurons has been studied in an attempt to reveal some of the mechanisms underlying excitatory transmission in the mammalian central nervous system. previous electrophysiological experiments have shown that the excitatory postsynaptic potentials (epsps) evoked dsct neurons by impulses in a single muscle afferent fluctuate in amplitude. these fluctuations occur between discrete amplitudes which are sep ... | 1985 | 2991483 |
| isolation and characterization of the human pulmonary surfactant apoprotein gene. | pulmonary surfactant is a phospholipid-protein complex which serves to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. inadequate levels of surfactant at birth, a frequent situation in premature infants, results in respiratory failure. in all species examined, surfactant is composed primarily of dipalmitoylphosphatidylcholine and two major protein species of relative molecular mass (mr) 32,000 (32k) and 10k (refs ... | 1985 | 2995821 |
| calcitonin receptors in the rat mesencephalon mediate its analgesic actions: autoradiographic and behavioral analyses. | autoradiographic analyses of salmon calcitonin (sct) binding in the rat mesencephalon revealed an exceptionally high concentration of receptors in the ventral and ventrolateral segments of the periaqueductal gray matter (pag) extending along the entire rostral-caudal axis. relatively heavy labeling was also seen along a band extending ventrolaterally through the mesencephalic reticular formation. other receptor-rich areas include the nucleus linearis, pars compacta and lateralis of the substanti ... | 1985 | 2996694 |
| mutations affecting gyrase in haemophilus influenzae. | mutants separately resistant to novobiocin, coumermycin, nalidixic acid, and oxolinic acid contained gyrase activity as measured in vitro that was resistant to the antibiotics, indicating that the mutations represented structural alterations of the enzyme. one novr mutant contained an altered b subunit of the enzyme, as judged by the ability of a plasmid, pnov1, containing the mutation to complement a temperature-sensitive gyrase b mutation in escherichia coli and to cause novobiocin resistance ... | 1985 | 2997115 |
| isolation and characterization of ca2+-blind mutants of yersinia pestis. | the plasmid pcd1 is required for expression of the low-calcium response (lcr), virulence, and production of v antigen in yersinia pestis kim. five independent mutants constitutive for the lcr at 37 degrees c (lcrc) were obtained through ethyl methanesulfonate mutagenesis followed by ampicillin enrichment. a sixth, spontaneous mutant was obtained directly through ampicillin enrichment. these mutants failed to grow at 37 degrees c regardless of calcium concentration and produced v antigen constitu ... | 1985 | 2997127 |
| rflp for the human apolipoprotein b gene: ii;ecori. | 1985 | 2997725 | |
| [isolation of yersinia pestis plasmids with transposon markers]. | transposons tn1, tn7, tn9, tn10 have been inserted into each of three known plasmids in yersinia pestis and have been shown to mutagenize the different plasmid genes. the marked plasmids are shown to be transduced by bacteriophage p1 cml clr 100 ts in intrageneric crosses. the genes of 61-65 md plasmid were found to be impaired with high frequencies by tn9 and tn10 insertions blocking the synthesis of fraction i antigen. the genes are also impaired in course of transduction of transposon marked ... | 1985 | 3025677 |
| evaluation of a photolabile derivative of 1,25-dihydroxyvitamin d3 as a photoaffinity probe for 1,25-dihydroxyvitamin-d3 receptor in chick intestinal cytosol. | we evaluated the viability of 1 alpha, 25-dihydroxyvitamin d3-3 beta-[n-(4-azido-2-nitrophenyl)glycinate] (1,25-(oh)2-d3-ang), an analog of 1 alpha, 25-dihydroxyvitamin d3 (1,25-(oh)2-d3) as a photoaffinity probe for 1,25-(oh)2-d3 receptor in chick intestinal cytosol. a competitive-binding assay revealed that chick intestinal cytosolic 1,25-(oh)2- d3 receptor bound to 1,25-(oh)2-d3-ang approximately 20-times less effectively than it did to 1,25-(oh)2-d3. irradiation of 1,25-(oh)2-d3- ang in the ... | 1985 | 2998355 |
| autoxidation reactions of hemoglobin a free from other red cell components: a minimal mechanism. | rates of autoxidation reactions are determined for normal human hemoglobin a preparations which are extensively purified to remove all other redox active red cell components. the effects of superoxide dismutase, catalase, and hydroxyl radical scavengers on the reaction provide evidence for superoxide formation as the rate determing step followed by fast reactions that involve peroxide and hydroxyl radical. these results support a minimum overall mechanism for heme iron(ii) oxidation and dioxygen ... | 1985 | 2998382 |
| collagen accumulation can continue with decreased prolyl hydroxylase activity in the liver. | a single dose of dimethylnitrosamine dose-relatedly increased total hepatic hydroxyproline content in rats 14 days after the dosing. in cases of 35 mg/kg of dimethylnitrosamine, it increased rapidly to 1.7 times the normal level within 14 days. this increase persisted thereafter until 84 days. hepatic collagen prolyl hydroxylase activity was 1.8 times the normal level by the fourth day after the dosing but normalized within 14 days. it decreased further to levels significantly lower than those i ... | 1985 | 2999546 |
| the effects of castration and/or methimazole feeding on the pituitary response to temperature extremes by cockerels. | the pituitary of goitrogen-treated white leghorn cockerels is smaller in size than control birds and the pituitaries of castrated cockerels is nearly twice the size of control birds. the pituitary cells generated by these treatments may not be functional thyrotrophs or gonadotrophs and may not be able to respond to their usual stimuli. low ambient temperature is a well-known stimulus to the thyroid gland acting through pituitary tsh. cyclic-amp-dependent protein kinase activity levels are used h ... | 1985 | 3000864 |
| the effects of castration and/or methimazole feeding on the pituitary response to temperature extremes by cockerels. | the pituitary of goitrogen-treated white leghorn cockerels is smaller in size than control birds and the pituitaries of castrated cockerels is nearly twice the size of control birds. the pituitary cells generated by these treatments may not be functional thyrotrophs or gonadotrophs and may not be able to respond to their usual stimuli. low ambient temperature is a well-known stimulus to the thyroid gland acting through pituitary tsh. cyclic-amp-dependent protein kinase activity levels are used h ... | 1985 | 3000864 |
| reactivation of latent murine cytomegalovirus from kidney. | cytomegalovirus (cmv) is presumed to cause latent infection, but the sites of infection are incompletely known. we propose that latent murine cytomegalovirus is present in kidney and may be reactivated by explanation. immunosuppressive agents and allogeneic stimuli may enhance this process. balb/c mice were infected 11 to 14 months previously with 10(5) pfu of smith strain murine cytomegalovirus intraperitoneally. kidneys from 15 infected and nine uninfected mice were washed, minced into 1 to 2 ... | 1985 | 3003444 |
| nucleotide sequence of the central non-transcribed spacer region of physarum polycephalum rdna. | in physarum polycephalum the rrna genes are present on linear extrachromosomal molecules, each containing two transcription units arranged as a giant palindrome. in the center of the molecule, between the two transcription units, is the 23-kb central spacer, previously shown to contain the replication origins and several regions of direct and inverted repeats. segments of all the repeats in the spacer have been sequenced and their overall organization determined. the entire spacer consists of re ... | 1985 | 3005112 |
| [mutagenic effect during transduction of (gm-km)r markers of the r323 plasmid in yersinia pestis]. | genes of resistance to some aminoglycoside antibiotics from plasmid r323 were transduced by bacteriophage p1 cml clr100 ts in yersinia pestis. the resistance markers were capable of insertion into the chromosome or plasmid in the recipient cells causing the mutagenic effect. the results obtained suggest the transposon nature of plasmid fragment coding for gentamicin-kanamycin resistance. | 1985 | 3025695 |
| non-immune igg f(ab')2 binding to group c and g streptococci is mediated by structures on gamma chains. | the present investigation was designed to determine whether the heavy or the light immunoglobulin chain is involved in the non-immune binding of igg f(ab')2 fragments to specific surface receptors on human group c and g streptococci. purified human polyclonal igg was mildly reduced with dithiothreitol and alkylated with iodoacetamide. light (l) and heavy (h) chains were separated. intact igg and purified l and h chains of polyclonal immunoglobulin g were tested in an inhibition assay for non-imm ... | 1985 | 3156400 |
| [inhibitory effects of bisphosphonates on the resorption of alveolar bone in rats]. | 1985 | 3161960 | |
| influenza viruses as lymphocyte mitogens. i. b cell mitogenesis by influenza a viruses of the h2 and h6 subtypes is controlled by the i-e/c subregion of the major histocompatibility complex. | influenza a viruses of the h2, h3, and h6 subtypes function as t cell-independent b cell mitogens for lymphocytes from balb/c mice. lymphocytes from c57bl/10 mice, however, undergo mitogenesis only in response to h3 viruses. the failure of c57bl/10 lymphocytes to respond to h2 and h6 viruses was shown not to reflect a difference in dose-response profile or kinetics of the response, nor was it due to the activity of suppressor t cells. experiments with congenic and recombinant strains of mice est ... | 1985 | 3155540 |
| [research extension in midwifery education (stockholm)]. | 1985 | 261495 | |
| [a case of endothoracic goiter]. | 1985 | 261941 | |
| new american dental association specification no. 32 for orthodontic wires not containing precious metals. council on dental materials and devices. | 1985 | 271683 | |
| presidential address. social changes and student nurses. | 1985 | 261352 | |
| attitudes to death and dying. | 1985 | 261501 | |
| phosphatases in the central nervous system of spiders (arachnida, araneae). | the central nervous systems of web-building spiders (araneidae, agelenidae) and hunting spiders (lycosidae, salticidae) were tested for non-specific and specific phosphatases. acid phosphatase exhibited weakly to moderately positive reactions in the neuronal cell bodies and in the neuropile fibre mass of all species investigated. alkaline phosphatase could only be demonstrated in the external and internal neural lamellae of the brain and ventral cord of several specimens of the araneid species i ... | 1985 | 218910 |
| genetics: the shape of things to come. | 1986 | 260179 | |
| [obesity in a walk-in consultation office]. | the weight, height, body mass index (bmi) and deviation from the ideal weight according to tables were measured in the patients over 24 years who spontaneously attended a demand clinic. these data permitted to calculate the prevalence of obesity in the demand clinic, and its relation with age, sex, frequentation rate and type of consultation were evaluated. 301 subjects were included, 113 males and 188 females, with a mean age of 53.43 +/- 10.68 years. according to their bmi, 52.62% were obese. ... | 1986 | 2129654 |
| legislative bulletin: the thrill of victory--the agony of defeat. | 1986 | 260967 | |
| the quality initiative of the royal college of general practitioners. | the quality initiative of the royal college of general practitioners was set up in 1983 to promote the methods of quality assurance to general practitioners, and to answer some of the concerns of government about the quality of general practice. many small projects have been carried out, and this paper describes several undertaken by one faculty of the college, including studies of paediatric surveillance, the care of the elderly, and peer review visits to some members practices. general practit ... | 1986 | 2490949 |
| [circulating immune complexes containing an insulin of bovine origin in a patient "allergic" to insulin]. | 1986 | 3302476 | |
| [the rodent. akodon arviculoides, wagner, 1842 (cricetidae)--importance in plague foci in brazil]. | the occurrence of the rodent akodon arviculoides wagner, 1842 in the plague focus of the "agreste" region of the state of pernambuco and a report on its ability for survival, reproduction and development in captivity, its susceptibility to yersinia pestis infection and the role of this rodent species in brazilian plague foci are reported. | 1986 | 3302594 |
| canine lymphoma: clinical considerations. | 1986 | 3333887 | |
| canine lymphoma: clinical considerations. | 1986 | 3333887 | |
| release of opioid peptides from the spinal cord of rats subjected to chronic pain. | chronic localized pain increased the level of the opioid peptides, dynorphin (dyn), alpha-neoendorphin (aneo), met-enkephalin (met) and mets-enkephalin-arg6-gly7-leu8 (meagl), in the lumbar enlargement of the rat spinal cord. it was accompanied with a reduction of the spontaneous and k+-stimulated release of aneo and meagl from spinal cord slices in vitro and a decreased release of aneo from the spinal cord in vivo. the results indicate that the reduction in the activity of endogenous opioid pep ... | 1986 | 3123959 |
| molecular biology of homo sapiens. | 1986 | 3088726 | |
| characterization of cyanobacterial ferredoxin-nadp+ oxidoreductase molecular heterogeneity using chromatofocusing. | chromatofocusing has been used as an analytical tool to check preparations of the enzyme ferredoxin-nadp+ oxidoreductase (ec 1.18.1.2) purified in either the presence or absence of the serine protease inhibitor phenylmethylsulfonyl fluoride from the cyanobacterium anabaena sp. strain 7119. only one isoelectric species was found when the crude extract was processed in the presence of the protease inhibitor. nevertheless, when the inhibitor was omitted, four ionic forms of the enzyme--showing appa ... | 1986 | 3089056 |
| standardization and evaluation of the clinical usefulness of mycobacterial skin test antigens. | 1986 | 3090420 | |
| galactosyltransferase purified from rat milk is distinct from the human and bovine enzyme. | n-acetylglucosaminide beta 1, 4-galactosyltransferase (ec 2.4.1.22) was purified from rat milk by affinity chromatography on n-acetylglucosamine-sepharose and alpha-lactalbumin-sepharose columns. the purified enzyme migrated as three polypeptides of relative molecular weight 59,000, 54,000, and 27,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. antiserum raised against the 54k rat protein immunoprecipitated all three polypeptides, suggesting that they share common antigenic sit ... | 1986 | 3091666 |
| calspectin (fodrin or nonerythroid spectrin)-actin interaction: a possible involvement of 4.1-related protein. | the calspectin/actin complex extracted from the bovine brain membrane crosslinks f-actin, resulting in the increasing viscosity of f-actin determined by low-shear viscometry. we demonstrated the presence of a protein factor in this complex, which regulated the calspectin-f-actin interaction in a ca2+- and calmodulin-dependent manner. erythrocyte protein 4.1, but not synapsin i, mimics the function of this brain factor using a reconstitution system including purified calspectin, calmodulin and f- ... | 1986 | 3096326 |
| primary structure of the paramecium tetraurelia small-subunit rrna coding region: phylogenetic relationships within the ciliophora. | we have sequenced the coding region for the small-subunit rrna gene from paramecium tetraurelia. similarity comparisons between small-subunit rrnas from representatives of the metazoa, the plantae, the fungi and four other members of the ciliophora were used to construct phylogenetic trees. in these phylogenies the ciliophora diverged from the eukaryotic line of descent as a loose phylogenetic grouping during a radiative period that gave rise to the fungi, the plantae and the metazoa. | 1986 | 3084799 |
| immunochemical evidences that hexachlorobenzene induces two forms of cytochrome p-450 in the rat liver microsomes. | induction by hexachlorobenzene (hcb) of the liver microsomal system of metabolism of xenobiotics has been studied in comparison with the inductions by phenobarbital (pb) and 3-methylcholanthrene (mc). it has been shown that hcb increases the content of cytochrome p-450 in the microsomes. like pb, hcb induces the activities of aminopyrine- and benzphetamine-n-demethylases. at the same time hcb increases also the activities of benzpyrenehydroxylase and 7-ethoxyresorufin-o-deethylase, which are cha ... | 1986 | 3085962 |
| survey of aminoglycoside resistance patterns. | we studied the susceptibility of 545 gram-negative clinical isolates to six aminoglycosides: gentamicin, tobramycin, netilmicin, amikacin, 2'-n-ethylnetilmicin, and 6'-n-ethylnetilmicin. the resistant strains (359) have been classified into aminoglycoside resistance (patterns) phenotypes (agrps) corresponding to known resistance mechanisms. the results clearly demonstrated that most strains of proteus spp., klebsiella spp., escherichia coli and pseudomonas aeruginosa appeared to be able to adeny ... | 1986 | 3087636 |
| gastric form of alpha chain disease. | a case of alpha chain disease, involving the stomach only, is reported in an algerian man suffering from epigastric pains. upper digestive tract fibreoptic endoscopy showed two antral ulcers and an ulcerative gastritis pattern, which promptly disappeared with cimetidine treatment. antral biopsies at a distance from the ulcers, but not of the ulcer crater itself, disclosed a dense infiltration of antral lamina propria by mature or sometimes atypical plasma cells. on transmural surgical antral bio ... | 1986 | 3087826 |
| comparison of three actin-coding sequences in the mouse; evolutionary relationships between the actin genes of warm-blooded vertebrates. | we have determined the sequences of three recombinant cdnas complementary to different mouse actin mrnas that contain more than 90% of the coding sequences and complete or partial 3' untranslated regions (3'utrs): pam 91, complementary to the actin mrna expressed in adult skeletal muscle (alpha sk actin); paf 81, complementary to an actin mrna that is accumulated in fetal skeletal muscle and is the major transcript in adult cardiac muscle (alpha c actin); and pal 41, identified as complementary ... | 1986 | 3084797 |
| comparative tests for detection of plague antigen and antibody in experimentally infected wild rodents. | the enzyme-linked immunosorbent assay (elisa) was compared with other standard tests for detection of plague (yersinia pestis) antibody and antigen in multimammate mice (mastomys coucha and m. natalensis) which were experimentally infected and then killed at daily intervals postinoculation. for detection of antibody in sera from m. natalensis, the immunoglobulin g (igg) elisa was equivalent in sensitivity to passive hemagglutination and more sensitive than the igm elisa and complement fixation. ... | 1986 | 3097065 |
| [combined efficacy of polyamine antimetabolites and cis-diamminedichloroplatinum]. | the combined antitumor effects of the polyamine antimetabolites, alpha-difluoro methylornithine (dfmo) and methylglyoxal-bis-guanylhydrazone (mgbg), with cddp were studied using human gastric cancer cells xenotransplanted into nude mice. dfmo (1000 mg/kg in two divided doses) and mgbg (50 mg/kg) were given ip for six consecutive days from the time when the xenotransplanted tumor weighted about 100 mg, and cddp (3.0 mg/kg) was given ip every other day from the same time. animals treated with dfmo ... | 1986 | 3100839 |
| antibodies to la, jo-1, nrnp and sm detected by multi-track immunoblotting using a novel filter holder: a comparative study with counterimmunoelectrophoresis and immunodiffusion using sera from patients with systemic lupus erythematosus and sjögren's syndrome. | the use of western blotting or immunoblotting to detect autoantibodies in the serum of patients with autoimmune connective tissue diseases was investigated. an apparatus suitable for simultaneously screening 16 sera on immunoblots was used to show that a complex pattern of antibody binding polypeptides was present in whole hela cells. a simpler and readily interpreted pattern of binding was achieved using affinity-purified rabbit thymus antigens. seventy-seven patients with systemic lupus erythe ... | 1986 | 3088125 |
| galactosyltransferase: multiple forms in serum of normal and hepatoma mc-29 bearing chickens and from liver and hepatoma microsomal and plasma membrane preparations. | the multiple forms of galactosyltransferase in chicken serum and in microsomal and plasma membrane preparations from liver and viral induced hepatoma mc-29 have been studied by isoelectric focussing. an elevation of the hepatoma plasma membrane enzyme activity was described and in the pattern of the multiple forms of the enzyme two forms were found (pi-5.34 and 8.22) which were similar to those described in the serum of hepatoma bearing chickens (pi-5.36 and 8.24). a conclusion is drawn that the ... | 1986 | 3102037 |
| comparative study of encainide and quinidine in the treatment of ventricular arrhythmias. | the antiarrhythmic efficacy and safety of oral encainide hydrochloride and quinidine sulfate were compared in a nine center double-blind crossover study in 187 outpatients with benign or potentially lethal ventricular arrhythmias. patients with at least 30 premature ventricular complexes/h were randomized to receive either encainide, 25 mg four times/day, or quinidine, 200 mg four times/day, for 2 weeks. these doses were continued for another 2 weeks if a 75% or greater reduction in premature ve ... | 1986 | 3079781 |
| effects of the binding of myosin light chain kinase on the reactivities of calmodulin lysines. | the effects of the binding of smooth muscle myosin light chain (mlc) kinase on the microenvironments of different regions of calmodulin (cam) were investigated by comparing the acylation rate constants of the seven lysine amino groups of free cam with those of cam complexed with mlc kinase. equimolar amounts of cam and cam-mlc kinase complex were trace labeled with [3h]acetic anhydride in the presence of phenylalanine as a standard nucleophile. after completion of the reaction, equal amounts of ... | 1986 | 3091599 |
| phenotypic characterisation of peripheral blood lymphoid cells in people exposed to fibrous zeolite. | among inhabitants of the village of karain in turkey there is an extremely high incidence of malignant mesothelioma, most probably due to exposure to erionite, which is a fibrous zeolite and similar in appearance and properties to asbestos. this mineral may be found in the dust in the village. to characterise possible disturbances in the immune system of people exposed to fibrous zeolite, a phenotypic characterisation of lymphoid cells in the peripheral blood of 74 immigrants to sweden from kara ... | 1986 | 3026434 |
| phenotypic characterisation of peripheral blood lymphoid cells in people exposed to fibrous zeolite. | among inhabitants of the village of karain in turkey there is an extremely high incidence of malignant mesothelioma, most probably due to exposure to erionite, which is a fibrous zeolite and similar in appearance and properties to asbestos. this mineral may be found in the dust in the village. to characterise possible disturbances in the immune system of people exposed to fibrous zeolite, a phenotypic characterisation of lymphoid cells in the peripheral blood of 74 immigrants to sweden from kara ... | 1986 | 3026434 |
| in vitro binding of an ige protein to human platelets. | bronchoconstriction in extrinsic asthma is initiated by mediators released from ige-sensitized leukocytes after contact with polyvalent antigen. because platelets also contain soluble mediators that can cause bronchoconstriction, platelet activation and release of the contents of platelet granules may play a role in ige-mediated responses under some circumstances. we therefore sought to determine if platelets are capable of binding ige and if cross-linking this cell-bound ige initiates secretion ... | 1986 | 3007618 |
| construction and characterisation of a series of multi-copy promoter-probe plasmid vectors for streptomyces using the aminoglycoside phosphotransferase gene from tn5 as indicator. | several versatile, multi-copy, promoter-probe plasmid vectors have been constructed that replicate in a wide range of streptomyces species. transcriptional activity is detected by the expression of a promoter-less aminoglycoside phosphotransferase gene (neo) derived from the transposon tn5; expression of this gene confers kanamycin and neomycin resistance on streptomyces lividans. an efficient transcriptional terminator from e. coli phage fd has been inserted upstream of the neo coding region to ... | 1986 | 3018431 |
| bradykinin stimulates gtp hydrolysis in ng108-15 membranes by a high-affinity, pertussis toxin-insensitive gtpase. | in membranes of neuroblastoma x glioma hybrid (ng108-15) cells, bradykinin (ec50 approximately equal to 5 nm) stimulates gtp hydrolysis by a high-affinity gtpase (km approximately equal to 0.2 microm). the octapeptide, des-arg9-bradykinin, was inactive. stimulation of gtp hydrolysis by bradykinin and an opioid agonist was partially additive. treatment of ng108-15 cells with pertussis toxin, which inactivates ni, eliminated gtpase stimulation by the opioid agonist but not by bradykinin. the data ... | 1986 | 3005034 |
| bradykinin stimulates gtp hydrolysis in ng108-15 membranes by a high-affinity, pertussis toxin-insensitive gtpase. | in membranes of neuroblastoma x glioma hybrid (ng108-15) cells, bradykinin (ec50 approximately equal to 5 nm) stimulates gtp hydrolysis by a high-affinity gtpase (km approximately equal to 0.2 microm). the octapeptide, des-arg9-bradykinin, was inactive. stimulation of gtp hydrolysis by bradykinin and an opioid agonist was partially additive. treatment of ng108-15 cells with pertussis toxin, which inactivates ni, eliminated gtpase stimulation by the opioid agonist but not by bradykinin. the data ... | 1986 | 3005034 |
| [ecology and epidemiology of transmissible viruses of sandflies in italy]. | 1986 | 3019208 | |
| susceptibility to varicella-zoster virus among adults at high risk for exposure. | the adult health care provider who is susceptible to varicella zoster virus (vzv) represents a risk to her or himself and to patients. nineteen percent of employees at this children's hospital had no or uncertain prior experience with vzv, and of these, 28% were found to be vzv susceptible, representing 5% of the total population of 2,730 hospital employees. during the 12 months of study, six of the potential 135 to 137 vzv-susceptible individuals acquired varicella. vzv-susceptible health care ... | 1986 | 3019915 |
| susceptibility to varicella-zoster virus among adults at high risk for exposure. | the adult health care provider who is susceptible to varicella zoster virus (vzv) represents a risk to her or himself and to patients. nineteen percent of employees at this children's hospital had no or uncertain prior experience with vzv, and of these, 28% were found to be vzv susceptible, representing 5% of the total population of 2,730 hospital employees. during the 12 months of study, six of the potential 135 to 137 vzv-susceptible individuals acquired varicella. vzv-susceptible health care ... | 1986 | 3019915 |
| in vitro and in vivo inhibition of cysteine proteinases by est, a new analog of e-64. | e-64 isolated from a culture of aspergillus japonicus is a specific inhibitor of cysteine proteinases. e-64-c, a synthetic analog of e-64, was effective in model animals of muscular dystrophy only when it was given intraperitoneally and by means of osmotic minipump. it showed no effects due to its low absorbability from intestine when it was administered orally. est, the ethyl ester of e-64-c, was expected to be readily absorbed through intestinal membrane, since it is more lipophilic than e-64- ... | 1986 | 3023601 |
| expression of simian virus 40 early and late genes in mouse oocytes and embryos. | simian virus 40 (sv40) large- and small-tumor antigens (t-ag, t-ag) are normally synthesized early after infection of either permissive (monkey) or nonpermissive (mouse) fibroblasts, whereas an equivalent amount of viral coat protein (v-ag) is observed late after infection of permissive cells and only after viral dna replication has occurred. to determine whether or not expression of these genes is regulated in the same manner during early mammalian development, sv40 dna was injected into the nu ... | 1986 | 3016324 |
| pain, increasing girth in healthy japanese man. | 1986 | 3021791 | |
| pain, increasing girth in healthy japanese man. | 1986 | 3021791 | |
| frostbite: experimental assessment of tissue damage using tc-99m pyrophosphate. work in progress. | we designed an experimental model using a new method of freezing to study the pathogenesis and treatment of frostbite. frostbite was simulated in a manner that closely resembles that which occurs in a natural environment. we used a radionuclide imaging technique to monitor the evolution and extent of tissue damage relative to temperature, rate of freezing, and controlled rewarming. characteristic sequential changes were demonstrated on sequential nuclear scans. nonperfusion, followed by perfusio ... | 1986 | 3020608 |
| inhibition and reversal of endotoxin-, aggregated igg- and paf-induced hypotension in the rat by sri 63-072, a paf receptor antagonist. | platelet activating factor (paf) given intravenously produces systemic hypotension in the rat. similar effects can be induced using endotoxin or heat-aggregated igg challenges, which are thought to involve endogenous paf release. extending this concept, we have examined the ability of the paf antagonist sri 63-072 to inhibit or reverse systemic hypotension induced with paf, heat-aggregated igg or endotoxin 0111-b4 in rats. at 100 ng kg-1 paf, there occurred a 38.6 +/- 5.1% decrease in carotid me ... | 1986 | 3019921 |
| inhibition and reversal of endotoxin-, aggregated igg- and paf-induced hypotension in the rat by sri 63-072, a paf receptor antagonist. | platelet activating factor (paf) given intravenously produces systemic hypotension in the rat. similar effects can be induced using endotoxin or heat-aggregated igg challenges, which are thought to involve endogenous paf release. extending this concept, we have examined the ability of the paf antagonist sri 63-072 to inhibit or reverse systemic hypotension induced with paf, heat-aggregated igg or endotoxin 0111-b4 in rats. at 100 ng kg-1 paf, there occurred a 38.6 +/- 5.1% decrease in carotid me ... | 1986 | 3019921 |
| effect of dietary ascorbic acid and vitamin e on metabolic changes in rats and guinea pigs exposed to pcb. | the effect of the addition of dietary ascorbic acid and/or vitamin e (all-rac-alpha-tocopheryl acetate) in rats and guinea pigs exposed to pcb (polychlorinated biphenyls) was studied. rats were fed diets containing one of three levels of vitamin e (30, 500 or 1000 mg/kg diet) with or without pcb (200 mg/kg diet). guinea pigs were fed diets containing pcb (40 mg/kg diet) with 200 or 1000 mg ascorbic acid/kg diet and/or 70 or 2000 mg vitamin e/kg diet. for rats fed pcb, ascorbic acid in urine was ... | 1986 | 3080560 |