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predictive value of urinary cultures in assessment of microbial colonization of ureteral stents.bacterial colonization of indwelling ureteral stents may serve as a nidus for bacteriuria in operations where stents are manipulated. the predictive value of urine cultures in the assessment of stent colonization was examined in 65 patients with indwelling ureteral stents.199910646680
decreased production of ampc-type beta-lactamases associated with the development of resistance to quinolones in citrobacter freundii strains.the effect of fluoroquinolones in citrobacter freundii strains that results in a decreased expression of cephalosporin-hydrolysing beta-lactamases was studied. resistance to broad-spectrum cephalosporins and penicillins in two c. freundii clinical isolates was associated with moderate production of chromosomal ampc-type-beta-lactamase in addition to changes in the outer membrane proteins profile with respect to wild-type c. freundii strains. ten quinolone-resistant mutants were derived from the ...199910647079
resistance to third-generation cephalosporins in adult gram-negative bacillary meningitis.ninety-three patients with gram-negative bacillary meningitis (gnbm) were identified at kaohsiung chang gung memorial hospital, over a period of 12 years. among them, eight showed resistance to third-generation cephalosporins, accounting for 9% of the total gnbm cases and 29% of the postneurosurgical gnbm cases. the resistant pathogens included acinetobacter baumannii, klebsiella pneumoniae, citrobacter freundii and morganella morganii. these eight patients, six males and two females aged 18-61 ...199910378134
live/dead baclight : application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water.a rapid epifluorescence staining method using the live/dead bacterial viability kit (baclight) was applied to estimate both viable and total counts of bacteria in drinking water. baclight is composed of two nucleic acid-binding stains: syto 9 and propidium iodide. syto 9 penetrates all bacterial membranes and stains the cells green, while propidium iodide only penetrates cells with damaged membranes, and the combination of the two stains produces red fluorescing cells. optimal incubation conditi ...199910395466
[a consideration on the results of nationwide surveillance of antimicrobial susceptibilities--gram-negative bacilli].the results of the semi-annual nationwide surveillance of antimicrobial susceptibilities, conducted by the japanese ministry of health and welfare during the period of january 1993 to july 1995, were analyzed for typical gram-negative bacilli in the purpose of provision of an index for antimicrobial selection. during these 3 years, escherichia coli, citrobacter freundii, enterobacter aerogenes and proteus mirabilis showed slightly increasing tendency in susceptibility to fosfomycin (fom) and cit ...199910396690
the bacteriology of neonatal septicaemia in ile-ife, nigeria.the incidence of septicaemia among neonates categorized as being at high risk was 55 per cent in ile-ife, nigeria. gram-positive organisms, specifically staphylococcus aureus, were predominant (33.8 per cent) among bacteria cultured from proven cases of septicaemia. other coagulase-negative staphylococci also contributed 21 per cent, with staphylococcus epidermidis occurring in 5 per cent of the isolates. listeria monocytogenes was cultured from 8.4 per cent of septic neonates. pseudomonas aerug ...199910401192
susceptibility to levofloxacin of clinical isolates of bacteria from intensive care and haematology/oncology patients in switzerland: a multicentre study.the objective of this study was to examine the susceptibility of clinical isolates to levofloxacin, a fluoroquinolone with extended activity against gram-positive bacteria, and other antibiotics in 12 swiss clinical microbiology laboratories using the nccls disc diffusion technique. isolates were prospectively collected from intensive care units (icus (59%), oncology wards (7%) and other units with haematology/oncology patients (34%) from june 1995 to march 1996. the levofloxacin breakpoints use ...199910404338
biochemical identification of citrobacter species defined by dna hybridization and description of citrobacter gillenii sp. nov. (formerly citrobacter genomospecies 10) and citrobacter murliniae sp. nov. (formerly citrobacter genomospecies 11).recent work describing six named species and two unnamed genomospecies within citrobacter has enlarged the genus to 11 species. dna relatedness and phenotypic tests were used to determine how well these species can be identified. one hundred thirty-six strains were identified to species level by dna relatedness and then identified phenotypically in a blinded fashion. by using conventional tests, 119 of the 136 strains (88%) were correctly identified to species level. three additional strains (2% ...199910405411
citrobacter freundii invades and replicates in human brain microvascular endothelial cells.neonatal bacterial meningitis remains a disease with unacceptable rates of morbidity and mortality despite the availability of effective antimicrobial therapy. citrobacter spp. cause neonatal meningitis but are unique in their frequent association with brain abscess formation. the pathogenesis of citrobacter spp. causing meningitis and brain abscess is not well characterized; however, as with other meningitis-causing bacteria (e.g., escherichia coli k1 and group b streptococci), penetration of t ...199910417193
role of bacterial intimin in colonic hyperplasia and inflammation.enteropathogenic escherichia coli (epec) cells adhere to gut epithelial cells through intimin alpha: the ligand for a bacterially derived epithelial transmembrane protein called the translocated intimin receptor. citrobacter rodentium colonizes the mouse colon in a similar fashion and uses a different intimin: intimin beta. intimin alpha was found to costimulate submitogenic signals through the t cell receptor. dead intimin beta+ c. rodentium, intimin alpha-transfected c. rodentium or e. coli st ...199910417389
[the clinico-morphological characteristics of salmonellosis typhimurium in children].clinical and morphological features were studied of the course of gastrointestinal and generalized forms of salmonellosis. the grave gastrointestinal form runs its course against the unfavourable premorbid background getting associated with other flora: proteus, citrobacter, klebsiella, staphylococcus aureus. a distinguishing feature of the generalized form is its variegated clinical symptomatology. noted in some instances is an acute onset of the illness with a clinical picture of purulent meni ...199910424015
biodegradation of tannic acid by citrobacter freundii isolated from a tannery effluent.a bacterial strain capable of utilizing tannic acid as sole carbon source was isolated from the effluent of a tannery and was identified as citrobacter freundii. this organism could grow at concentrations as high as 5% (w/v) of tannic acid and produced extracellular tannase to hydrolyze the same. when grown in minimal medium containing 1% tannic acid (w/v) at 30 degrees c, this strain produced 1.87 u/ml of tannase at 6 h. at that time, tannic acid degradation products, namely glucose and gallic ...199910427737
in vitro and in vivo activities of syn2190, a novel beta-lactamase inhibitor.syn2190, a monobactam derivative containing 1,5-dihydroxy-4-pyridone as the c-3 side chain, is a potent inhibitor of group 1 beta-lactamase. the concentrations of inhibitor needed to reduce the initial rate of hydrolysis of substrate by 50% for syn2190 against these enzymes were in the range of 0.002 to 0.01 microm. these values were 220- to 850-fold lower than those of tazobactam. syn2190 showed in vitro synergy with ceftazidime and cefpirome. this synergy was dependent on the concentration of ...199910428909
role of permeability in the activities of beta-lactams against gram-negative bacteria which produce a group 3 beta-lactamase.the production of a group 3 beta-lactamase permitted escherichia coli to raise the mics of ceftazidime, cefpirome, and meropenem greatly but those of imipenem and piperacillin only slightly. the ratios of maximum rate of hydrolysis to k(m) of ceftazidime, cefpirome, and piperacillin were lower than those of meropenem and imipenem for the group 3 beta-lactamase. the permeability coefficients for piperacillin and meropenem were higher than those for ceftazidime and cefpirome. imipenem had the high ...199910428944
use of pna oligonucleotides for the in situ detection of escherichia coli in water.a species-specific peptide nucleic acid (pna) oligonucleotide probe directed against the v(1)region of the 16s rrna molecule was synthesized for the detection of escherichia coli in water. the specificity of the probe was tested in dot blot hybridizations against a number of environmental isolates including those from the genera escherichia, klebsiella, enterobacter and citrobacter. in situ hybridization experiments were performed with biotinylated pna oligonucleotide probes with subsequent dete ...199910441198
in vitro activity of newer broad spectrum beta-lactam antibiotics against enterobacteriaceae and non-fermenters: a report from austrian intensive care units. austrian carbapenem susceptibility surveillance group.we compared the in vitro activity of broad spectrum beta-lactam antibiotics against 573 gram-negative isolates (enterobacteriaceae and non-fermenters) collected between november 1996 and may 1997 from 9 laboratories serving intensive care units throughout austria. mic's (minimal inhibitory concentration) were obtained with the e-test for meropenem, imipenem, ceftazidime, cefepime, cefpirome and piperacillin/tazobactam. pseudomonas aeruginosa was the most frequently isolated organism (22%), follo ...199910467641
[bacteriological and clinical break points: evaluation of the usefulness of cefozopran based on mic].drug sensitivity of clinically isolated bacteria to cefozopran (czop), which is a new cephem antibiotic, was investigated, and the usefulness of the drug was evaluated from the viewpoint of bacteriological and clinical (pneumonia) break points. the following results were obtained. 1. according to bacteriological break points, methicillin-resistant staphylococcus aureus (mrsa) and enterococcus spp. showed low sensitivity to cefozopran (czop). however, the sensitivity of methicillin-sensitive stap ...199910480051
[citrobacter freundii endocarditis].infection by citrobacter appears in man only in certain circumstances, since it usually acts as contaminant or colonizer. bacteraemia by this bacillus can affect immunodeficient people, elderly people or those patients who have undergone invasive hospital processes. although incidence of bacteraemia is low (0.3-0.9%), the death rate is very high, about 48%. this bacillus is seldom the cause of endocarditis. that is why we describe a case of endocarditis by citrobacter freundii, in an aged person ...199910481339
phylogenetic analysis of l4-mediated autogenous control of the s10 ribosomal protein operon.we investigated the regulation of the s10 ribosomal protein (r-protein) operon among members of the gamma subdivision of the proteobacteria, which includes escherichia coli. in e. coli, this 11-gene operon is autogenously controlled by r-protein l4. this regulation requires specific determinants within the untranslated leader of the mrna. secondary structure analysis of the s10 leaders of five enterobacteria (salmonella typhimurium, citrobacter freundii, yersinia enterocolitica, serratia marcesc ...199910498727
quorum-sensing cross talk: isolation and chemical characterization of cyclic dipeptides from pseudomonas aeruginosa and other gram-negative bacteria.in cell-free pseudomonas aeruginosa culture supernatants, we identified two compounds capable of activating an n-acylhomoserine lactone (ahl) biosensor. mass spectrometry and nmr spectroscopy revealed that these compounds were not ahls but the diketopiperazines (dkps), cyclo(deltaala-l-val) and cyclo(l-pro-l-tyr) respectively. these compounds were also found in cell-free supernatants from proteus mirabilis, citrobacter freundii and enterobacter agglomerans [cyclo(deltaala-l-val) only]. although ...199910510239
the use of chromagar orientation as a primary isolation medium with presumptive identification for the routine screening of urine specimens.the aim of the study was to compare the use of a novel differential culture medium chromagar, for both primary isolation and presumptive identificaton, with the method currently used in our laboratory for screening mid-stream-urine samples (msu). routine methods (rm) included blotting paper imprinting of all specimens and additional quantitative culture on cysteine lactose electrolyte-deficient agar (cled) for selected samples together with microbact 12e for further identification. the chromagar ...199910519322
in vitro susceptibility to pexiganan of bacteria isolated from infected diabetic foot ulcers.during two clinical trials involving the treatment of 835 outpatients with infected diabetic foot ulcers, 2515 bacterial isolates, including 2337 aerobes and 178 anaerobes, were grown from cultures of the ulcers. the in vitro susceptibility of these isolates was determined to pexiganan, a peptide anti-infective evaluated in these clinical trials, and to other classes of antibiotics. pexiganan demonstrated broad spectrum antimicrobial activity against gram-positive and gram-negative aerobes and a ...199910529881
multicenter evaluation of the antimicrobial activity for seven broad-spectrum beta-lactams in turkey using the etest method. turkish antimicrobial resistance study group.from march through july 1997, a nine laboratory surveillance project was initiated in turkey to monitor the potency and spectrum of seven broad-spectrum antimicrobial agents (cefepime, ceftazidime, cefotaxime, imipenem, aztreonam, cefoperazone/sulbactam, and ticarcillin/clavulanate) tested against approximately 100 organisms (average 82; range 70 to 95 isolates) per participant center (736 strains). eleven groups of organisms were tested by the etest method (ab biodisk, solna, sweden) with resul ...199910529883
citrobacter urinary tract infections in children.citrobacter species have been described as the etiologic agents in cases of bacteremia, meningitis, diarrhea and brain abscess, but little is known of their role as a cause of urinary tract infections in children. the purpose of this study was to define the role of citrobacter species in pediatric urinary tract infections.199910530585
citrobacter rodentium espb is necessary for signal transduction and for infection of laboratory mice.citrobacter rodentium is the causative agent of transmissible murine colonic hyperplasia and contains a locus of enterocyte effacement (lee) similar to that found in enteropathogenic escherichia coli (epec). epec espb is necessary for intimate attachment and signal transduction between epec and cultured cell monolayers. mice challenged with wild-type c. rodentium develop a mucosal immunoglobulin a response to espb. in this study, c. rodentium espb has been cloned and its nucleotide sequence has ...199910531262
the distribution of enteric bacteria from australian mammals: host and geographical effects.bacteria of the family enterobacteriaceae were isolated from 642 mammalian hosts, representing 16 families and 79 species, collected from throughout australia. escherichia coli was the most common of the 24 enteric species recovered and represented almost half of the isolates. association analysis revealed that most other species of bacteria were less likely to be recovered from hosts in which e. coli was present. the composition of the enteric community of a host was found to be determined by b ...199910537188
the genetic structure of enteric bacteria from australian mammals.a total of 246 isolates representing five species of the family enterobacteriaceae, taken from a variety of australian mammal species, were characterized using multi-locus enzyme electrophoresis. genome diversity estimates varied significantly among species, with the klebsiella pneumoniae sample exhibiting the lowest diversity and the citrobacter freundii sample the highest. multi-locus linkage disequilibrium estimates revealed that alleles were non-randomly associated in all five species sample ...199910537189
regulation of inducible ampc beta-lactamase expression among enterobacteriaceae.ampc ss-lactamases are active-site serine enzymes that are primarily cephalosporinases. in many gram negative organisms, including enterobacter spp.,citrobacter freundii, serratia marcescens, morganella morganii and pseudomonas aeruginosa, the expression of chromosomal ampc genes is low but inducible in response to ss-lactams and other stimuli. the current working model for ampc induction requires exposure of bacterial cells to ss-lactam drugs or other stimuli and is linked to the cell wall recy ...199910539994
structural studies of the o-specific polysaccharide of hafnia alvei strain pcm 1207 lipopolysaccharide.the structure of the o-specific side-chain of the hafnia alvei strain pcm 1207 lipopolysaccharide (lps) has been investigated. methylation analysis, partial acid hydrolysis, matrix-assisted laser-desorption ionization time-of-flight (maldi-tof) ms, fast atom bombardment (fab)-ms/ms and 1h- and 13c-nmr spectroscopy were the principal methods used. glycerol phosphate was identified as a constituent in the polysaccharide and the following structure of a pentasaccharide repeating unit was establishe ...199910542050
the phylogenetic position of serratia, buttiauxella and some other genera of the family enterobacteriaceae.the phylogenetic relationships of the type strains of 38 species from 15 genera of the family enterobacteriaceae were investigated by comparative 16s rdna analysis. several sequences of strains from the genera citrobacter, erwinia, pantoea, proteus, rahnella and serratia, analysed in this study, have been analysed previously. however, as the sequences of this study differ slightly from the published ones, they were included in the analysis. of the 23 enterobacterial genera included in an overvie ...199910555323
detection and reporting of organisms producing extended-spectrum beta-lactamases: survey of laboratories in connecticut.extended-spectrum beta-lactamases (esbls) are enzymes produced in some gram-negative bacilli that mediate resistance to extended-spectrum cephalosporins and aztreonam. they are most common in klebsiella spp. and escherichia coli but are present in a variety of enterobacteriaceae. resistance mediated by these enzymes can be difficult to detect depending on the antimicrobial agents tested. ampc beta-lactamases are related to the chromosomal enzymes of enterobacter and citrobacter spp. and also med ...199910565933
epidemic infection caused by citrobacter rodentium in a gerbil colony.non-motile, gram-negative rods, isolated from the intestinal tract and kidney of several dead animals in a gerbil colony, were identified as citrobacter rodentium (formerly included in c. freundii species) on the basis of 31 biochemical tests. the isolates were tested against 40 antimicrobial agents and were all susceptible to ticarcillin plus clavulanate, ceftazidime and most of the quinolones studied, but were all resistant to most of the penicillins and aminoglycosides tested, and to fosfomyc ...199910574274
in vitro activity of trovafloxacin (cp 99,219), a new fluoroquinolone against hospital isolates.the susceptibility of 492 enterobacteriaceae, 227 other gram-negative bacteria, 448 gram-positive bacteria and 108 anaerobic organisms was determined by the agar dilution method against trovafloxacin and other antibiotics. trovafloxacin was highly active against most of the enterobacteriaceae including enterobacter spp. and citrobacter spp. [minimum inhibitory concentration (mic)90 <1 mg/l], acinetobacter spp. and pseudomonas aeruginosa (mic90 = 0.25 and 2 mg/l, respectively). the antimicrobial ...19999876206
cloning and expression in escherichia coli of an azoreductase gene from clostridium perfringens and comparison with azoreductase genes from other bacteria.a genomic library of clostridium perfringens atcc 3626 was constructed in phage lambda gt11 and screened with an antibody against the c. perfringens azoreductase, which catalyzes the reduction of azo dyes to aromatic amines. a positive recombinant phage, containing a 3.8 kb dna fragment insert was selected and purified. lytic and lysogenic escherichia coli cultures infected with the recombinant phage had higher azoreductase activity than cultures infected only with the vector lambda gt11. the 3. ...199910071864
synthesis and evaluation of 2 beta-oxyimino and alkenylpenicillanic acid sulfone derivatives as beta-lactamase inhibitors.the synthesis and in vitro synergies of 2 beta-alkenyl and oxyiminopenam sulfone derivatives are described. most of the compounds synthesized exhibited good inhibitory activities and synergistic antibacterial activities with piperacillin and ceftriaxone, respectively, against several beta-lactamase producing strains. particularly the 2 beta-alkenylpenam sulfone derivatives. 1e and 1g, showed good synergistic activity with ceftriaxone against citrobacter freundi nih 10018-68 and proteus vulgaris ...199910073138
[priapism secondary to a bilateral abscess of the corpora cavernosa].presentation of an exceptional case of priapism in an 82-year-old male, secondary to primary bilateral abscess corpus cavernosum. diagnosis was reached through aspiration puncture and pus drainage, which also allowed the resolution of the condition. after a literature review, no similar case was found. this was a painful, long-lasting erection that resulted from corpus cavernosum repletion with purulent material instead of blood.199910089636
[spontaneous bacterial peritonitis due to citrobacter freundii]. 199910089713
conversion of tyrosine phenol-lyase to dicarboxylic amino acid beta-lyase, an enzyme not found in nature.tyrosine phenol-lyase (tpl), which catalyzes the beta-elimination reaction of l-tyrosine, and aspartate aminotransferase (aspat), which catalyzes the reversible transfer of an amino group from dicarboxylic amino acids to oxo acids, both belong to the alpha-family of vitamin b6-dependent enzymes. to switch the substrate specificity of tpl from l-tyrosine to dicarboxylic amino acids, two amino acid residues of aspat, thought to be important for the recognition of dicarboxylic substrates, were graf ...19999880502
characterization of sfo-1, a plasmid-mediated inducible class a beta-lactamase from enterobacter cloacae.enterobacter cloacae 8009 produced an inducible class a beta-lactamase which hydrolyzed cefotaxime efficiently. it also hydrolyzed other beta-lactams except cephamycins and carbapenems. the activity was inhibited by clavulanic acid and imipenem. the bla gene was transferable to escherichia coli by electroporation of plasmid dna. the molecular mass of the beta-lactamase was 29 kda and its pi was 7.3. all of these phenotypic characteristics of the enzyme except for inducible production resemble th ...19999925524
a novel chromogenic ester agar medium for detection of salmonellae.a novel agar medium, chromogenic salmonella esterase (cse) agar, for the differentiation of salmonellae is described. the agar contains peptones and nutrient extracts together with the following (grams per liter unless otherwise specified): 4-[2-(4-octanoyloxy-3, 5-dimethoxyphenyl)-vinyl]-quinolinium-1-(propan-3-yl carboxylic acid) bromide (slpa-octanoate; bromide form), 0.3223; lactose, 14. 65; trisodium citrate dihydrate, 0.5; tween 20, 3.0; ethyl 4-dimethylaminobenzoate, 0.035% (wt/vol), novo ...19999925620
antibiotic policy: a tool for controlling resistance of hospital pathogens.multiresistant gram-negative bacilli, including strains of klebsiella pneumoniae, enterobacter spp, acinetobacter baumannii and pseudomonas aeruginosa, resistant to broad spectrum beta-lactams, aminoglycosides and fluoroquinolones, are recovered at increasing frequency from patients suffering from nosocomial infections, particularly from those receiving intensive care. the emergence and spread of resistant pathogens to endemic and epidemic levels has frequently been related in time and place to ...199911869273
in vitro susceptibility of gram-negative bacterial isolates to chlorhexidine gluconate.to investigate the susceptibility of clinical isolates of gram-negative bacteria to chlorhexidine gluconate.199910750501
citrobacter diversus urosepsis and cerebral abscess in a child with antenatal hydronephrosis.one percent of all pregnancies are found to have an antenatal abnormality; of these, 20% involve the genitourinary system. today, controversy still exists regarding the postnatal management of some antenatal abnormalities detected by ultrasound. we present a case in which antenatal hydronephrosis initially detected by ultrasound appeared to resolve in utero. postnatally, the child developed citrobacter diversus urosepsis, meningitis, and cerebral abscess. voiding cystourethrogram obtained after ...199910754158
isolation of yersinia enterocolitica from cases of acute appendicitis and ice-cream.seventy (70) appendiceal specimens and 80 ice-cream samples were analysed to detect yersinia enterocolitica using three different media. both y. enterocolitica and citrobacter freundii were recovered in appendiceal specimens (17.1% and 8.6%) and ice-cream (26.25% and 18.75%) respectively. thioglycollate medium was more selective and productive in isolating yersinia. y. enterocolitica was the major causative agent of acute appendicitis (11/25, 44%). it was sensitive to chloramphenicol, gentamicin ...199910793790
a bacterial study of pyoderma in belgaum.one hundred children with primary pyoderma and 50 healthy children were included in the study. the swabs collected from the lesions were cultured on various media. the colonies were identified by conventional methods. commonest isolate was staph. aureus (45%), followed by strep. pyogens (35%), e. coli (5%), citrobacter (1%) and staph. and strept. together (14%). staphylococci showed highest resistance to ampicillin (85%), followed by penicillin (78%), tetracycline (40%) and ciprofloxacin (15%). ...199920885049
moxifloxacin (bay 12-8039): a new methoxy quinolone antibacterial.moxifloxacin (bay 12-8039) is a new 8 methoxy quinolone antibacterial. the mic90 values are < or = 0.25 mg/l for streptococcus pneumoniae (irrespective of penicillin susceptibility), haemophilus influenzae (beta-lactamase positive or negative), morexella catarrhalis, bordetella pertussis, legionella sp., mycoplasma pneumoniae, clamydia pneumoniae, mycobacterium tuberculosis, methicillin-sensitive staphylococcus aureus, beta-haemolytic streptococci (macrolide-sensitive or -resistant), listeria sp ...199915992072
characterization of a bioflocculant produced by citrobacter sp. tkf04 from acetic and propionic acids.a bacterial strain, tkf04, capable of producing a bioflocculant from acetic and/or propionic acids was isolated from a biofilm formed in inside a kitchen drain. it was identified as a citrobacter based on its morphological and physiological characteristics and the partial sequences of its 16s rrna. tkf04 produced the bioflocculant during the logarithmic phase of growth, and the optimum temperature and ph for the bioflocculant production were 30 degrees c and 7.2-10.0, respectively. it could util ...200016232696
bacteriological examination of milk and milk products sold in harare.a study to assess the bacteriological quality of milk and ice cream was conducted using the direct plate count method and the methylene blue dye reduction test. a total of 105 milk and 95 ice cream samples were obtained form two factories (depots) and distributing supermarkets (outlets) in harare. under the methylene blue test, all milk and ice cream samples passed the hour and 2 hour tests respectively. however, 99% of the milk and 69% of the ice cream samples reduced the dye after 5.5 and 4 hr ...200017650038
a note on escherichia coli o157:h7 serotype in turkish meat products.255 minced beef, 101 soudjouk and 50 uncooked hamburger samples were analyzed for the presence of escherichia coli o157:h7 serotype. m-ec and lst broths were used as selective enrichment media and smac agar was used as a selective isolation medium. a total of 3 e. coli o157 were isolated by conventional culture techniques; one from each of minced beef, uncooked hamburger and soudjouk but none were identified as the h7 serotype. for determination of selective media-growing cohabitant bacteria, 26 ...200022062162
structure and serologic properties of o-specific polysaccharide from citrobacter freundii possessing cross-reactivity with escherichia coli o157:h7.citrobacter freundii ocu158 is a serologically cross-reactive strain with escherichia coli o157:h7. to explore the close relationship between two strains, we have analyzed the chemical structures of o-specific polysaccharides and antigenic properties of lipopolysaccharides (lpss) of both strains. the structure of o-specific polysaccharides from both strains was found to be identical by chemical and nuclear magnetic resonance analyses, in which d-pernac was 4-acetamido-4,6-dideoxy-d-mannose: [--> ...200010799808
murine colonic mucosa hyperproliferation. i. elevated cftr expression and enhanced camp-dependent cl(-) secretion.fluid transport in the large intestine is mediated by the cystic fibrosis gene product and camp-dependent anion channel cystic fibrosis transmembrane conductance regulator (cftr). camp-mediated cl(-) secretion by gastrointestinal cell lines in vitro has been positively correlated with the insertion of cftr into the apical membrane of differentiated senescent colonocytes and negatively correlated with the failure of cftr to insert into the plasma membrane of their undifferentiated proliferating c ...200010801268
tuberculous false aneurysm of the femoral artery managed by endoluminal stent graft insertion. 200010801384
[the structure and antibiotic sensitivity of the causative agents of community-acquired infectious diseases of bacterial origin in children].four hundred and forty pediatric patients at the age of 7 days to 15 years with various infections admitted to the hospital within a month were examined. the biological material was inoculated to blood agar on the first days of the patient admittance to the hospital and after the growth the organisms were isolated and identified. antibiotic susceptibility of the isolates was assayed with the disk diffusion method. 479 strains in all were tested. the most frequent cases requiring hospitalization ...200010768061
citrobacter freundii tyrosine phenol-lyase: the role of asparagine 185 in modulating enzyme function through stabilization of a quinonoid intermediate.asn185 is an invariant residue in all known sequences of tpl and of closely related tryptophanase and it may be aligned with the asn194 in aspartate aminotransferase. according to x-ray data, in the holoenzyme and in the michaelis complex asn185 does not interact with the cofactor pyridoxal 5'-phosphate, but in the external aldimine a conformational change occurs which is accompanied by formation of a hydrogen bond between asn185 and the oxygen atom in position 3 of the cofactor. the substitutio ...200010775663
ascending cholangitis as a cause of pyogenic liver abscesses complicated by a gastric submucosal abscess and fistula.ruptures of nonamebic (pyogenic) liver abscesses into the thorax and peritoneum are very uncommon; but, hepatoduodenal and hepatocolonic fistulas are ever more rare. we report a case where ascending cholangitis was associated with pyogenic liver abscess formation and a gastric fistula. drainage into the stomach was demonstrated by gastroduodenal endoscopy for gastric bleeding. after fistula formation, we could successfully treat the inflammation caused by infection of citrobacter freundii and ca ...200010777197
[detection of extended spectrum beta-lactamases producing enterobacteriaceae in feces].a study was made of 366 feces for detection of extended spectrum beta-lactamases producing enterobacteriaceae from feces. the selective agar was used for modified drigalski agar (eiken chemical co., ltd) with 2 micrograms/ml cefotaxime (esbl screen agar). 92 strains of enterobacteriaceae, 41 escherichia coli, 15 citrobacter freundii, 13 enterobacter cloacae, 11 klebsiella pneumoniae, and other 12, were isolated from esbl screen agar. and, r-plasmid that were selected by 2 micrograms/ml cefotaxim ...200010783580
treatment of sanitary-important bacteria by bacteriocin substance v24 in cattle dung water.quantification of sanitary-important bacteria (e.g. enterobacteriaceae), as well as indicators of environmental contamination, was assessed in samples of cattle dung from 25 cattle farms in 15 north-eastern slovakia districts. the inhibitory effect of crude bacteriocin extract cbe v24 from enterococcus faecalis v24 against listeria monocytogenes ohio and yersinia enterocolitica ye85 was examined in cattle dung water with the aim of finding a new way of eliminating the health risk of the animal s ...200010792671
type 1 pili of citrobacter freundii mediate invasion into host cells. 200011109110
orbital abscess: management and outcome.to discuss the diagnosis, management and outcome of various types of orbital abscess.200011116509
susceptibility of bacterial isolates to gatifloxacin and ciprofloxacin from clinical trials 1997-1998.mics of gatifloxacin and ciprofloxacin against 3482 pre-treatment, clinical trial isolates collected during 1997-1998 are reported. these data suggested that gatifloxacin was four- to eight-fold more active than ciprofloxacin against gram-positive bacteria, with gatifloxacin mic(90)s < or = 0.33 mg/l against staphylococcus aureus and streptococcus pneumoniae, and < or = 1.0 mg/l versus viridans streptococci and enterococcus faecalis. both quinolones had similar mic(90)s versus enterobacteriaceae ...200011118848
bacterial agents of lower respiratory tract infections (lrtis), beta-lactamase production, and resistance to antibiotics in elderly people. dedalo study group.this study determined the etiology of lower respiratory tract infections in the elderly and assessed whether the growth of beta-lactamase producing bacteria is particularly favoured in these patients. between december 1998 and may 1999, 187 patients with community-acquired pneumonia (cap), and 887 patients with acute exacerbations of chronic obstructive pulmonary disease (aecopd) were enrolled. the mean age was 74 years (range of 65-94 year). sputum and bronchial aspirate for microbiological inv ...200011118860
comparison of the escherichia coli k-12 genome with sampled genomes of a klebsiella pneumoniae and three salmonella enterica serovars, typhimurium, typhi and paratyphi.the escherichia coli k-12 genome (eco) was compared with the sampled genomes of the sibling species salmonella enterica serovars typhimurium, typhi and paratyphi a (collectively referred to as sal) and the genome of the close outgroup klebsiella pneumoniae (kpn). there are at least 160 locations where sequences of >400 bp are absent from eco but present in the genomes of all three sal and 394 locations where sequences are present in eco but close homologs are absent in all sal genomes. the 394 s ...200011121489
improvement of mannitol lysine crystal violet brilliant green agar for the selective isolation of h2s-positive salmonella.mannitol lysine crystal violet brilliant green agar (mlcb) is widely used in japan for salmonella isolation because the medium has been commercially available. colonies of salmonella on mlcb appear colorless with black centers due to h2s gas production; however, most citrobacter freundii also produce h2s gas. in order to distinguish h2s-positive salmonella from c. freundii we have improved mlcb. to mlcb was added 1% lactose (l-mlcb). the relation for ph and black center colony formation was exam ...200011131884
emergence of domestically acquired ceftriaxone-resistant salmonella infections associated with ampc beta-lactamase.ceftriaxone, an expanded-spectrum cephalosporin, is an antimicrobial agent commonly used to treat severe salmonella infections, especially in children. ceftriaxone-resistant salmonella infections have recently been reported in the united states, but the extent of the problem is unknown.200011135779
citrobacter diversus endocarditis.citrobacter species are motile gram-negative bacilli that cause disease in humans, such as urinary tract infection, pneumonia, superficial and deep wound infections, gastroenteritis, meningitis, bacteremia, and rarely endocarditis. in those cases of endocarditis, intravenous drug use has been associated with citrobacter species. gram-negative organisms are present in less than 10% of cases of endocarditis in intravenous drug users. we present a case of tricuspid valve endocarditis in an intraven ...200011149556
[diagnostic value of a novel nutrient medium for isolation and cultivation of pathogens causing enteric yersiniosis and pseudotuberculosis].a new nutrient medium for isolation and cultivation of the causative agents of enteric yersiniosis and pseudotuberculosis was found to have advantages over endo medium in its differentiating and inhibiting properties. this medium permitted the easy differentiation of yersinia pseudotuberculosis from y. enterocolitica, as well as from escherichia coli, shigella flexneri, klebsiella pneumoniae, k. rhinoscleromatis, hafnia, enterobacter and citrobacter by color; from proteus inconstans by swarming. ...200011210625
[in vitro antibacterial activity of prulifloxacin, a new oral quinolone, and comparative susceptibility rate at clinical breakpoint mic].in vitro drug sensitivity of clinically isolated bacteria against prulifloxacin (pufx), which is a new quinolone, was investigated, and the antibacterial activity and susceptibility rate at clinical breakpoint were compared with those of norfloxacin, ofloxacin (oflx), ciprofloxacin, tosufloxacin, fleroxacin, sparfloxacin and levofloxacin (lvfx). the following results were obtained. 1) pufx showed a broad-spectrum antibacterial activity against both gram-positive and gram-negative bacteria. 2) mi ...200011214999
comparison of antimicrobial susceptibility of citrobacter freundii isolates in two different time periods.citrobacter freundii was first identified in 1932, since then it has been reported to cause a variety of infections in aged, immunocompromised, and debilitated patients. with the use of broad-spectrum antibiotics, c. freundii has become increasingly resistant to antimicrobial agents. in order to determine the chronological changes in susceptibility and current susceptibility status of c. freundii, we compared the antimicrobial susceptibility of c. freundii in two different time periods, from 198 ...200011269372
mechanical carrier of bacterial enteric pathogens by chrysomya megacephala (diptera: calliphoridae) in chiang mai, thailand.chrysomya megacephala was studied regarding its mechanically bacterial carrier in urban areas of chiang mai, northern thailand. fifty-six adult flies were randomly collected using sweep insect net during april-may, 1999 from 3 fresh food markets and examined for bacteriological isolation. among them, 49 flies (87.5%) were bacterial carriers. the total 22 bacterial species and 8 groups were isolated. three species previously reported as the bacterial enteric pathogens causing diarrheal disease we ...200011414447
liver hematoma following endoscopic retrograde cholangiopancreatography (ercp).we report the case of an 81-year-old man who presented with abdominal pain following endoscopic retrograde cholangiopancreatography (ercp) for choledocholithiasis. a diagnosis of infected hematoma was made. a ct-guided puncture produced bloody matter that grew citrobacter freundii. a catheter was left in place for 3 weeks before the patient could be discharged from hospital. we hypothesize that the hepatic parenchyma had been torn by the guide used during the ercp. this case represents the first ...200011287996
[multicenter evaluation of the antimicrobial activity in vitro for six broad-spectrum beta-lactams in china using the e-test method].to investigate the in vitro activities of cefepime and other 5 beta-lactam antimicrobial agents against 937 strains isolated from 10 teaching hospitals in different geographical areas in china during the early 1998.200011798789
resolution of high-molecular-weight components in lipopolysaccharides of escherichia coli, morganella morganii, citrobacter freundii and citrobacter diversus strains with sodium dodecyl sulfate polyacrylamide gels.the use of 0.5% sodium dodecyl sulfate in polyacrylamide separation gels allowed the resolution in several bands of high-molecular-mass components in smooth lipopolysaccharide of bacterial outer membrane from escherichia coli, morganella morganii, citrobacter freundii and citrobacter diversus. with or without 0.1% sds, however, such a result was not possible.200010576704
fragmentation of 23s rrna in strains of proteus and providencia results from intervening sequences in the rrn (rrna) genes.intervening sequences (ivss) were originally identified in the rrl genes for 23s rrna (rrl genes, for large ribosomal subunit, part of rrn operon encoding rrna) of salmonella enterica serovars typhimurium lt2 and arizonae. these sequences are transcribed but later removed during rnase iii processing of the rrna, resulting in fragmentation of the 23s species; ivss are uncommon, but have been reported in at least 10 bacterial genera. through pcr amplification of ivs-containing regions of the rrl g ...200010648538
association of qace and qacedelta1 with multiple resistance to antibiotics and antiseptics in clinical isolates of gram-negative bacteria.clinical isolates of enterobacter cloacae, citrobacter freundii, pseudomonas aeruginosa, and stenotrophomonas maltophilia were tested for resistance to antibiotics and to the antiseptics benzalkonium chloride and cetyltrimethylammonium bromide. furthermore, they were examined for the presence of the resistance genes qace and qacedelta1. qacedelta1 was detected by pcr in 10% of all (n=103) and in 81% of multiply antibiotic-resistant strains (n=15). qace was found in only one of 37 p. aeruginosa s ...200010650208
convenient test for screening metallo-beta-lactamase-producing gram-negative bacteria by using thiol compounds.a simple disk diffusion test was constructed for detection of imp-1-type metallo-beta-lactamase-producing gram-negative bacteria. two kirby-bauer disks containing ceftazidime (caz) and a filter disk containing a metallo-beta-lactamase inhibitor were used in this test. several imp-1 inhibitors such as thiol compounds including 2-mercaptopropionic acid, heavy metal salts, and edta were evaluated for this test. two caz disks were placed on a mueller-hinton agar plate on which a bacterial suspension ...200010618060
[community-acquired bacteremic pneumonia due to citrobacter diversus]. 200010804650
e. coli invasion of brain microvascular endothelial cells as a pathogenetic basis of meningitis.a major limitation to advances in prevention and therapy of bacterial meningitis is our incomplete understanding of the pathogenesis of this disease. successful isolation and cultivation of bmec, which constitute the blood brain barrier, and the development of experimental hematogenous meningitis animal model, which mimics closely the pathogenesis of human meningitis, enabled us to dissect the pathogenetic mechanisms of bacterial meningitis. we have shown for the first time using e. coli as a pa ...200010804851
molecular beacons: a real-time polymerase chain reaction assay for detecting salmonella.molecular beacons are oligonucleotide probes that become fluorescent upon hybridization. we developed a real-time pcr assay to detect the presence of salmonella species using these fluorogenic reporter molecules. a 122-base-pair section of the hima was used as the amplification target. molecular beacons were designed to recognize a 16-base-pair region on the amplicon. as few as 2 colony-forming unit (cfu) per pcr reaction could be detected. we also demonstrated the ability of the molecular beaco ...200010805535
pk(a) calculations for class c beta-lactamases: the role of tyr-150.the poisson-boltzmann method was used to compute the pk(a) values of titratable residues in a set of class c beta-lactamases. in these calculations, the pk(a) of the phenolic group of residue tyr150 is the only one to stand out with an abnormally low value of 8.3, more than one pk(a) unit lower than the measured reference value for tyrosine in solution. other important residues of the catalytic pocket, such as the conserved lys67, lys315, his314, and glu272 (hydrogen-bonded to the ammonium group ...200010813827
in vitro activity of gemifloxacin against a broad range of recent clinical isolates from the usa.the antibacterial potencies of gemifloxacin (sb-265805) and 13 comparator compounds were determined by broth microdilution against a panel of 645 gram-positive and 995 gram-negative organisms collected from various usa sites. time-kill studies were performed and postantibiotic effect (pae) was determined for several organisms using trovafloxacin and ciprofloxacin as comparator compounds. based on mic(90)s, gemifloxacin was the most potent compound tested against gram-positive isolates: streptoco ...200010824027
prevalence of salmonella spp. in poultry broilers and shell eggs in korea.this study was conducted to determine the presence of salmonella spp. in raw broilers and shell eggs in korea. in total, 135 dozen shell eggs and 27 raw broilers were tested. none of the egg yolks were found to contain salmonella organisms but escherichia coli, escherichia hermanii, and citrobacter freundii were isolated from egg shells. salmonella spp. were detected in 25.9% of raw broilers, and salmonella serotypes isolated from raw broilers were salmonella enteritidis, salmonella virchow, and ...200010826725
enteropathogenic escherichia coli isolates in paediatric diarrhoea.enteropathogenic escherichia coli (epec) are known to cause infantile enteritis. we studied the prevalence of epec in paediatric patients with acute and persistent diarrhoea. a total of 56 stool samples from paediatric patients were studied. there were 28 significant bacterial isolates. of these 21 were untypable e. coli, 5 were typable e. coli, four of which belonged to members considered to be enteropathogenic. non e. coli isolates grown in pure culture were one each of pseudomonas aeruginosa ...200010835962
[accuracy of antibiograms performed directly from urine incubation bottles of the sebac system for diagnosing bacteriuria]. 200010846244
a kinetic study on the interaction between tazobactam (a penicillanic acid sulphone derivative) and active-site serine beta-lactamases.the interaction between tazobactam and several chromosome- and plasmid-encoded (tem, shv, pse types) class a and c beta-lactamases was studied by spectrophotometry. tazobactam behaved as a competitive inhibitor or inactivator able to restore in several cases the efficiency of piperacillin as a partner beta-lactam. a detailed kinetic analysis permitted measurement of the acylation efficiency for some cephalosporinases and broad-spectrum beta-lactamases; the presence of a turn-over of acyl-enzyme ...200010850951
in vitro efficacy of six cephalosporins tested against enterobacteriaceae isolated at 38 north american medical centres participating in the sentry antimicrobial surveillance program, 1997-1998.the sentry antimicrobial surveillance program is an ongoing international collaboration that monitors the predominant bacterial and fungal pathogens and antimicrobial susceptibility patterns associated with community-acquired and nosocomial infections. sentry data on the current cephalosporin susceptibility patterns (1997-98) of north american isolates of clinically important enterobacteriaceae were analyzed. susceptibility to a selection of cephalosporins was assessed at a central laboratory us ...200010854806
a new rapid automated method for the detection of listeria from environmental swabs and sponges.many food and meat processors test environmental swabs and sponges to confirm the absence of listeria spp. spectral pattern changes in a liquid growth medium, resulting from esculin hydrolysis by listeria in contaminated swabs and sponges, were automatically monitored by the biosys instrument in a semifluid layer (sfl). the blackening of sfl in modified mox broth resulted in sharply declining curves, which were easily detected by the instrument. the instrument detected all nine strains of lister ...200010857550
a novel ctx-m beta-lactamase (ctx-m-8) in cefotaxime-resistant enterobacteriaceae isolated in brazil.to estimate the diversity of extended-spectrum beta-lactamases in brazil, 18 strains from different species of the family enterobacteriaceae exhibiting a positive double-disk synergy test were collected by a clinical laboratory from several hospitals in rio de janeiro, brazil, in 1996 and 1997. four strains (proteus mirabilis, enterobacter cloacae, enterobacter aerogenes, and citrobacter amalonaticus) hybridized with a 550-bp ctx-m probe. the p. mirabilis strain produced a ctx-m-2 enzyme. the e. ...200010858358
in vitro activity of rifaximin, metronidazole and vancomycin against clostridium difficile and the rate of selection of spontaneously resistant mutants against representative anaerobic and aerobic bacteria, including ammonia-producing species.rifaximin is a rifamycin derivative characterized by a wide antibacterial activity. this drug is neither absorbed by the gastrointestinal tract nor inactivated by gastric juices, and exerts its action entirely within the intestinal lumen.200010859431
cefditoren activity against nearly 1000 non-fastidious bacterial isolates and the development of in vitro susceptibility test methods.cefditoren (formerly me-1206) is an investigational, orally administered cephalosporin ester with bactericidal activity against many gram-positive and -negative organisms. cefditoren potency against nearly 1000 non-fastidious species was determined by national committee for clinical laboratory standards (nccls) reference broth microdilution and standardized disk diffusion methods. against staphylococci, usable cefditoren activity was completely correlated with oxacillin with respect to potency a ...200010863109
modification of the bile salts-irgasan-brilliant green agar for enumeration of aeromonas species from food.the present study evaluated the productivity of bibg medium for the isolation of aeromonas spp. from food and describes a modification of the bibg medium (mbibg) (increased ph (8.7), replacement of xylose by soluble starch as a carbon source, decreased concentration of bile salts) to increase its selectivity and electivity. using the mbibg medium, growth of the majority of the enterobacteriaceae (9/10) was suppressed except for citrobacter freundii. the mbibg medium supported growth of pseudomon ...200010868682
a cycle: recurrent gram-negative folliculitis with citrobacter diversus (koseri) following eradication of recurrent staphylococcal pyoderma. 200010871958
the immune responses to bacterial antigens encountered in vivo at mucosal surfaces.mammals have evolved a sophisticated immune system for handling antigens encountered at their mucosal surfaces. the way in which mucosally delivered antigens are handled influences our ability to design effective mucosal vaccines. live attenuated derivatives of pathogens are one route towards the development of mucosal vaccines. however, some molecules, described as mucosal immunogens, are inherently immunogenic at mucosal surfaces. studies on mucosal immunogens may facilitate the identification ...200010874742
citrobacter sepsis and severe newborn respiratory failure supported with extracorporeal membrane oxygenation.an infant with fulminant citrobacter sepsis and respiratory failure is presented. the severity of respiratory failure and the need for systemic heparinization on extracorporeal membrane oxygenation delayed the opportunity of initial lumbar puncture to rule out meningitis. the infant was successfully treated with extracorporeal membrane oxygenation and long-term antibiotics. repeated cranial computed tomography scans remained negative for intracerebral abscesses, and the infant is within normal l ...200010879343
assessment of the microbiological quality and wash treatments of lettuce served in university restaurants.one hundred and forty-four samples of lettuce from 16 university restaurants were analyzed. the mesophilic aerobic counts of all samples ranged from 3.01 to 7.81 log10 cfu g(-1). results of total coliforms ranged from < 0.47 to > 3.38 log10 most probable number (mpn) g(-1). of the lettuce samples, 25.7% harbored escherichia coli, 22.9% staphylococcus aureus and 84% group d streptococci. similarly, 10.4% of the samples harbored aeromonas hydrophila, 2.8% pseudomonas aeruginosa, and coliforms such ...200010898470
increased nuclear translocation of catalytically active pkc-zeta during mouse colonocyte hyperproliferation.protein kinase (pk) c-zeta is implicated in the control of colonic epithelial cell proliferation in vitro. however, less is known about its physiological role in vivo. using the transmissible murine colonic hyperplasia (tmch) model, we determined its expression, subcellular localization, and kinase activity during native crypt hyperproliferation. enhanced mitosis was associated with increased cellular 72-kda holoenzyme (pkc-zeta, 3.2-fold), 48-kda catalytic subunit (pkm-zeta, 3- to 9-fold), and ...200010898766
expression of intimin gamma from enterohemorrhagic escherichia coli in citrobacter rodentium.the carboxy-terminal 280 amino acids (int280) of the bacterial adhesion molecule intimin include the receptor-binding domain. at least five different types of int280, designated alpha, beta, gamma, delta, and epsilon, have been described based on sequence variation in this region. importantly, the intimin types are associated with different evolutionary branches and contribute to distinct tissue tropism of intimin-positive bacterial pathogens. in this study we engineered a strain of citrobacter ...200010899867
[enterobacterial native valve endocarditis in the intensive care unit: report of two cases]. 200010909160
the role of glutamic acid-69 in the activation of citrobacter freundii tyrosine phenol-lyase by monovalent cations.tyrosine phenol-lyase (tpl) from citrobacter freundii is activated about 30-fold by monovalent cations, the most effective being k(+), nh(4)(+), and rb(+). previous x-ray crystal structure analysis has demonstrated that the monovalent cation binding site is located at the interface between subunits, with ligands contributed by the carbonyl oxygens of gly52 and asn262 from one chain and monodentate ligation by one of the epsilon-oxygens of glu69 from another chain [antson, a. a., demidkina, t. v. ...200010913261
[diagnosis of malacoplakia in a transplanted kidney by needle biopsy].malakoplakia is an uncommon inflammatory condition rarely involving parenchyma of transplanted kidney. we report a 44-year-old female recipient of a cadaveric renal allograft treated with cyclosporin a and prednisone. after transplantation, e. coli and citrobacter bacteruria occurred and three years later decreased graft function developed. percutaneous needle biopsy was performed and diagnosis of malakoplakia was established. histologically, interstitial sheets of plasmocytes and histiocytes wi ...200010916930
isolations of enteric pathogens from synanthropic flies trapped in downtown kuala lumpur.four species of synanthropic flies were trapped in downtown kuala lumpur: chrysomya megacephala, chrysomya rufifacies, musca domestica, and musca sorbens. burkholderia pseudomallei, the organism causing melioidosis, was the dominant bacteria isolated from chrysomya megacephala. klebsiella oxytoca, commonly associated with nosocomial infections, was commonly isolated from chrysomya megacephala, musca domestica, and musca sorbens. aeromonas hydrophila, the bacteria causing gastroenteritis, was pre ...200010925800
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