Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| agglutination of bordetella species by lectins. | phase i cells of bordetella pertussis but not those of b. parapertussis, b. bronchiseptica or b. avium were agglutinated by limulus polyphemus lectin. most strains of b. pertussis but not those of the other species were also agglutinated by helix pomatia lectin. in precipitation reactions between lectins and purified bordetella lipopolysaccharide (lps) preparations a similar pattern occurred. lectin agglutination provides a rapid presumptive method for the differentiation of b. pertussis from b. ... | 1992 | 1555759 |
| potency testing of acellular pertussis vaccines. | the laboratory assessment of the potential of acellular pertussis vaccines to protect against human disease is a major problem. the mouse intracerebral challenge test, which is the accepted potency assay for whole cell pertussis vaccines, is not suitable for testing acellular vaccines, and more recently developed murine respiratory infection assays have methodological drawbacks and doubtful relevance to the human infection. we have found that the ability of several bordetella pertussis antigens ... | 1992 | 1557927 |
| comparison of erythromycin estolate and erythromycin ethylsuccinate for treatment of pertussis. the erythromycin study group. | in an open randomized multicenter study 190 culture-positive pediatric ambulatory pertussis patients were treated for 14 days with either erythromycin estolate (est) (n = 93; 40 mg/kg/day divided in 2 doses) or erythromycin ethylsuccinate (eth) (n = 97; 60 mg/kg/day divided in 3 doses). on day 14 bordetella pertussis was recovered from cultures of 2 patients (2.2%) treated with est and 1 patient (1.0%) treated with eth. despite the fact that 151 patients (79.4%) had reached the early paroxysmal ... | 1992 | 1565532 |
| a simple and sensitive elisa of antibodies to pertussis antigens. | a precise method for quantifying serum antibody levels to antigens of bordetella pertussis is required to evaluate pertussis vaccines, to diagnose pertussis infections, and to detect transplacentally transmitted maternal antibodies. the purpose of this paper is to report a rapid, sensitive, easy and standardized elisa method using polystyrene balls for the assay of serum antibodies against pertussis toxin (pt) and filamentous haemagglutinin (fha), which are protective antigens of b. pertussis. b ... | 1992 | 1574921 |
| the role of histidine 63 in the catalytic mechanism of bordetella pertussis adenylate cyclase. | of the 9 histidines located in the catalytic domain of bordetella pertussis adenylate cyclase, three (his63, his106, and his298) were found to be conserved in the adenylate cyclase of bacillus anthracis, another calmodulin-dependent enzyme. substitution of his63 with arg, glu, gln, or val decreased the catalytic efficiency of adenylate cyclase between 2 and 3 orders of magnitude and altered the kinetic properties of the enzyme. these effects varied in relation to the nature of the substituting r ... | 1992 | 1577816 |
| effect of anti-dnp igg1- and igg2a-secreting hybridomas in vivo on the development of an anti-dnp ige antibody response in mice. | we reported previously that cba mice pretreated with dinitrophenyl-bordetella pertussis (dnp-bp) conjugates exhibited sharply decreased anti-dnp ige, and increased igg2a antibodies following immunization with dnp-ovalbumin (dnp-oa) in alum. the objective of the present experiments was to determine whether the decrease in anti-dnp ige was attributed to a regulatory effect exerted by igg2a antibodies. anti-dnp monoclonal antibodies (mab) of the igg1 or igg2a isotype were passively transferred to m ... | 1992 | 1582706 |
| comparative roles of the arg-gly-asp sequence present in the bordetella pertussis adhesins pertactin and filamentous hemagglutinin. | pertactin and filamentous hemagglutinin (fha), proteins present on the surface of the gram-negative organism bordetella pertussis, have been shown to contain the putative cell-binding sequence arginine-glycine-aspartic acid (rgd) and to promote eukaryotic cell attachment. the attachment of epithelial cells to purified pertactin and the entry of b. pertussis into human hela cells are both inhibited by an rgd-containing peptide derived from the pertactin sequence. in contrast, an rgd-containing pe ... | 1992 | 1587605 |
| outer membrane proteins and lipopolysaccharides of nontypeable haemophilus influenzae. | several outer membrane proteins of nontypeable haemophilus influenzae are potential vaccine candidates: p2 and p6 elicit antibodies that are bactericidal and protective in experimental models of infection. other proteins are being investigated. a group of surface-exposed high-molecular-weight proteins that are major targets of antibody in human convalescent sera were identified. monoclonal antibodies to the high-molecular-weight proteins of a prototype strain recognized two distinct but related ... | 1992 | 1588159 |
| serum antibodies against a 69-kilodalton outer-membrane protein, pertactin, from bordetella pertussis in nonvaccinated children with and without a history of clinical pertussis. | serum antibodies against pertactin were found in all samples from 260 nonvaccinated healthy swedish 1- to 4-year-old children, although 42% had neither a history of clinical pertussis nor antibodies against pertussis toxin or filamentous hemagglutinin. pertactin antibody levels were, however, higher in children with a history of pertussis than in those without a history of pertussis, and higher in children with antibodies against pertussis toxin and filamentous hemagglutinin than in those withou ... | 1992 | 1593352 |
| targeted mutations that ablate either the adenylate cyclase or hemolysin function of the bifunctional cyaa toxin of bordetella pertussis abolish virulence. | bordetella pertussis, the causative agent of whooping cough, secretes several toxins implicated in this disease. one of these putative virulence factors is the adenylate cyclase (ac) toxin that elevates intracellular camp in eukaryotic cells to cytotoxic levels. this toxin is a bifunctional protein comprising both ac and hemolysin (hly) enzymatic domains. the gene encoding the ac toxin (cyaa) is expressed as part of an operon that includes genes required for secretion or activation of the toxin. ... | 1992 | 1594590 |
| [evolution and treatment of 19 cases of pertussis in infants under 4 months of age]. | we present 19 cases of pertussis in infants under 4 months of age. in all cases, bordetella pertussis was isolated from nasopharyngeal swabs. a whooping cough, cyanotic episodes and eating disturbances were the most characteristic clinical findings. treatment included: supportive care, salbutamol, beclomethasone, and josamycin. lymphocyte counts higher than 40,000/mm2 were associated with a greater severity of illness. the most frequent complications were: recurrent cough and pneumonia. none of ... | 1992 | 1605413 |
| pertussis toxin and target eukaryotic cells: binding, entry, and activation. | pertussis toxin, a protein virulence factor produced by bordetella pertussis, is composed of an a protomer and a b oligomer. the a protomer consists of a single polypeptide, termed the s1 subunit, which disrupts transmembrane signaling by adp-ribosylating eukaryotic g-proteins. the b oligomer, containing five polypeptides, binds to cell receptors (most likely containing carbohydrate) and delivers the s1 subunit. current knowledge suggests that expression of adp-ribosyltransferase activity in tar ... | 1992 | 1612292 |
| both adenylate cyclase and hemolytic activities are required by bordetella pertussis to initiate infection. | among virulence factors synthesized and secreted by bordetella pertussis, pertussis toxin (ptx) and the bifunctional adenylate cyclase-hemolysin (ac-hly) are able to invade mammalian cells and to impair intracellular functions. moreover, both proteins are protective antigens in murine intracerebral and respiratory models. in order to study their in vivo properties, different b. pertussis mutants, deficient in ac-hly expression or secretion, or producing modified ac-hly devoid of either adenylate ... | 1992 | 1614333 |
| bordetella pertussis adenylate cyclase toxin. structural and functional independence of the catalytic and hemolytic activities. | the bordetella pertussis calmodulin-dependent adenylate cyclase (cyaa) is a 1706-residue-long toxin, endowed with hemolytic activity. we have constructed b. pertussis mutant strains producing modified cyaas devoid of adenylate cyclase activity. our results show that such modified cyaas display hemolytic activity identical to the wild-type toxin, thus demonstrating that the hemolytic activity is independent of the adenylate cyclase activity. furthermore, b. pertussis and escherichia coli strains ... | 1992 | 1618862 |
| detection of antibodies inhibiting the adp-ribosyltransferase activity of pertussis toxin in human serum. | bordetella pertussis produces a protein virulence factor termed pertussis toxin. many candidate pertussis vaccines are based on the rationale that an immune response that neutralizes the virulence activities of this toxin, which are thought to arise from its catalytic adp-ribosyltransferase activity, would be beneficial. the report describes two methods that quantify the inhibition of this activity by human serum. one, termed a direct assay, involves an initial incubation of toxin with serum, a ... | 1992 | 1624552 |
| modulation of adenylate cyclase toxin production as bordetella pertussis enters human macrophages. | during the course of human infection, bordetella pertussis colonizes sequential niches in the respiratory tract that include intracellular and extracellular environments. in vitro the expression of virulence factors such as the adenylate cyclase toxin is coordinately regulated by the bvg locus, which is an example of a two-component sensory transduction system. with this toxin as a reporter, enzyme activities were compared between a wild-type and an altered strain to determine whether bacterial ... | 1992 | 1631152 |
| [seroepidemiology of whooping cough in mexico]. | this paper presents the results from a serological survey developed in mexico during 1987 in order to estimate the prevalence of anti bordetella pertussis antibodies. agglutinins were measured in 25,666 samples taken from children one to 15 years old living throughout the country. in each case survey covered some data about social and demographic variables such as socioeconomic stratum, number of dpt vaccine doses received and urban or rural settlement, among others. titers greater than = 1.16 a ... | 1992 | 1631731 |
| trypanosoma cruzi: involvement of igg isotypes in the parasitemia control of mice immunized with parasite exoantigens of isoelectric point 4.5. | in a previous work we demonstrated that trypanosoma cruzi exoantigens of pi 4.5 (ea 4.5), whose most important epitopes are glucidic, are able to induce a partially protective immune response in mice. to ascertain the involvement of antibody isotypes in this protection, we immunized mice with ea 4.5 plus bordetella pertussis as adjuvant. the analysis of immune response by skin test revealed the occurrence of specific immediate type hypersensitivity on day 15 after the last immunization. by elisa ... | 1992 | 1639159 |
| a uniform alteration in serum lipid metabolism occurring during inflammation in mice. | the present study was designed to delineate changes in serum lipid levels following various kinds of tissue injury or inflammation such as contact sensitivity to picryl chloride, thermal burn, carrageenin-induced edema, the administration of turpentine oil, freund's complete adjuvant (fca), killed bordetella pertussis (bp) or lipopolysaccharide (lps). a uniform change in the serum lipid metabolism was observed in mice that received these inflammatory stimuli; that is, increases in total choleste ... | 1992 | 1640661 |
| epidermal growth factor and transforming growth factor alpha stimulate or inhibit proliferation of a human renal adenocarcinoma cell line depending on cell status: differentiation of the two pathways by g protein involvement. | transforming growth factor alpha production by renal tumors, acting through the epidermal growth factor receptor, has been implicated in malignant transformation by studies which compared gene expression in neoplastic and normal human tissue. we sought confirmation of this hypothesis by measuring the growth responses of a human renal tumor cell line to the addition of epidermal growth factor and transforming growth factor alpha. surprisingly, it was found that both growth factors could induce ei ... | 1992 | 1643633 |
| the epidemiology of pertussis in england and wales. | recovery in the uptake of whole-cell pertussis vaccine from 30% in 1978 to 91% in 1992 has resulted in a major reduction in the incidence of pertussis and in the proportion of cases occurring in pre-school children. this has been accompanied by a decline in pertussis mortality rates, with no deaths reported since september 1990. seventy-four per cent of the 50 fatal cases reported since 1980 have been infants under one year of age, demonstrating the importance of completing primary immunisation ... | 1992 | 1285134 |
| immunosuppressive effects induced by the polysaccharide moiety of some bacterial lipopolysaccharides. | the immunomodulatory properties of several lipopolysaccharides (lps) derived from clinical isolates of pseudomonas aeruginosa, branhamella catarrhalis, and bordetella pertussis were evaluated for their capacity to influence the magnitude of the antibody response to type iii pneumococcal polysaccharide (sss-iii), which is known to be regulated by suppressor and amplifier t cells (ts and ta, respectively). the administration of lps, two days after immunization resulted in a significant increase in ... | 1992 | 1286878 |
| epidemiology of pertussis and reactions to pertussis vaccine. | it remains clear that pertussis is a dangerous infectious disease that is well-controlled in industrialized countries by widespread immunization. in the developing world, it remains a source of high morbidity and mortality because of previously inadequate immunization programs. however, because of the intense efforts of the world health organization's expanded programme on immunization, the effects of pertussis have already been ameliorated and show promise of being within a decade of approximat ... | 1992 | 1289114 |
| bordetella pertussis extract induces increase in the activities of glycolytic enzymes in mouse liver. | the hypoglycemic effect of bordetella pertussis (challenge strain no.18323) purified cell extract (protein with traces of carbohydrates, 2 mg%) administered (0.1 mg/100 g body wt. i.v.) into mice on the activities of the key regulatory enzymes, viz. glucokinase, phosphofructokinase, pyruvate kinase, glyceraldehyde phosphodehydrogenase, glucose-6-phosphate dehydrogenase (g-6-pd) and lactate dehydrogenase, of glycolytic pathway in liver has been studied at varying intervals after injection. the ma ... | 1992 | 1289237 |
| cloning and sequence analysis of the evgas genes involved in signal transduction of escherichia coli k-12. | we have cloned and sequenced new escherichia coli genes which belong to member of the family of environmentally responsive two-component system and named evga and evgs because their amino acid sequences were found the most homologus to the bordetella pertussis bvga and bvgs. they were mapped at 51 min. and extending from 6b9 to 7g9 in the kohara miniset library of the e. coli chromosome. in fact, both evga and evgs proteins predicted from their dna sequences were identified in the in vitro coupl ... | 1992 | 1289796 |
| laboratory correlates of protection and protective immunity to bordetella pertussis. | 1992 | 1295347 | |
| effects of ketotifen on airway responses to allergen challenge in the actively sensitized brown norway rat. | the purpose of the study was to examine the effect of ketotifen on the airway responses and the recruitment of the inflammatory cells into the airways of sensitized rats after antigen challenge. twenty-five brown norway rats, 7-9 weeks old, were actively sensitized to ovalbumin (oa) (1 mg s.c.) and bordetella pertussis vaccine (10(9) bacilli i.p.). at 14 days after sensitization rats were anesthetized with urethane (1.1 g/kg i.p.) and intubated endotracheally. aerosols of oa (5% w/v in saline fo ... | 1992 | 1295373 |
| bordetella pertussis fha antibodies in maternal/infants sera and colostrum. | the high incidence of pertussis in the first year of life confirms that susceptibility remains high for children in this age group despite > 90% pertussis vaccine compliance. in this respect, immunoresponse to bordetella pertussis was investigated. filamentous hemagglutinin (fha) antibodies were studied due to their important protective role, in blocking the adherence of the bacteria to respiratory tract ciliated cells. the relative rate of detection and degree of positivity of igg and iga antib ... | 1992 | 1295946 |
| siderophore production and membrane alterations by bordetella pertussis in response to iron starvation. | bordetella pertussis was grown in iron (fe)-free defined medium to limit the growth of the organism. doubling times of the fe-starved organism increased by approximately 1 h, and a 40% reduction in the final extent of growth in fe-depleted medium was observed. under these conditions, a hydroxamate siderophore named bordetellin was secreted by b. pertussis. lactoferrin and transferrin supported growth of b. pertussis even when the protein was sequestered inside dialysis tubing. this suggested tha ... | 1992 | 1309510 |
| signal transduction during antibody-dependent cellular cytotoxicity mediated by u937 cells. | stimulation of the human promonocytic cell line u937 with antibody-coated chicken red blood cells (ab-crbc), leads to inositol phosphate (ip) release in the effector cells. neomycin (5 x 10(-4) m) completely inhibits activation of phosphoinositide breakdown, while adcc is suppressed in a dose-dependent manner. bordetella pertussis toxin (pt) (0.5 micrograms/ml), entirely inhibits ip release, while adcc activity is markedly suppressed. the pkc inhibitors h-7 and propranolol also suppress adcc. ha ... | 1992 | 1312511 |
| bordetella pertussis induces respiratory burst activity in human polymorphonuclear leukocytes. | virulent bordetella pertussis strains survive intracellularly within human polymorphonuclear leukocytes (pmn), at least in part because of inhibition of phagosome-lysosome fusion (l. l. steed, m. setareh, and r. l. friedman, j. leukocyte biol. 50:321-330, 1991). further investigations were done to determine if b. pertussis also inhibited respiratory burst activity of pmn as an additional mechanism of intracellular survival. chemiluminescence and flow cytometry assays showed that b. pertussis ind ... | 1992 | 1314225 |
| adenosine 3',5'-monophosphate suppresses metastatic spread in nude mice of steroidogenic rat granulosa cells transformed by simian virus-40 and ha-ras oncogene. | 8-bromo-camp and substances elevating camp levels within cells, such as forskolin, cholera toxin, and bordetella pertussis-invasive adenylate cyclase (bpac), suppress the growth of cultured granulosa cells cotransfected by simian virus-40 (sv40) dna and ha-ras oncogene concomitantly with the induction of steroidogenesis and without affecting oncogene expression. we, therefore, tested the hypothesis that camp can modulate tumorigenesis and metastatic spread of these cells in vivo. the cotransfect ... | 1992 | 1319328 |
| enzymatic activity of adenylate cyclase toxin from bordetella pertussis is not required for hemolysis. | adenylate cyclase (ac) toxin from bordetella pertussis enters cells to cause supraphysiologic increases in camp. ac toxin is also hemolytic. substitution of lys-58 with a methionine residue by site-directed mutagenesis of the structural gene for ac toxin, cyaa, and introduction of this mutation onto the b. pertussis chromosome results in an organism that synthesizes an enzyme-deficient ac toxin molecule. this mutant toxin molecule exhibits 1000-fold reduction in enzymatic activity relative to wi ... | 1992 | 1319923 |
| isolation and molecular characterization of a novel broad-host-range plasmid from bordetella bronchiseptica with sequence similarities to plasmids from gram-positive organisms. | a 2.6 kb plasmid, named pbbr1, was isolated from bordetella bronchiseptica s87. after insertion of an antibiotic resistance marker, this plasmid could be transferred into escherichia coli, bordetella pertussis, b. bronchiseptica, vibrio cholerae, rhizobium meliloti, and pseudomonas putida by transformation or conjugation. conjugation was possible only when the incp group transfer functions were provided in trans. as shown by incompatibility testing, pbbr1 does not belong to the broad-host-range ... | 1992 | 1321324 |
| trans-acpd inhibits camp formation via a pertussis toxin-sensitive g-protein. | in primary cultured striatal neurons we found that (+-)-trans-1-amino-cyclopentyl-1,3-dicarboxylate (trans-acpd) could inhibit forskolin-induced camp formation in a dose-dependent manner (ec50 156 +/- 38 microm, n = 5, maximal inhibition 37.8 +/- 1.2, n = 37). the trans-acpd-induced inhibition was totally abolished in neurons preincubated with bordetella pertussis toxin (1 microgram/ml), demonstrating the involvement of a g-protein. this is the first report in intact neurons of a glutamate metab ... | 1992 | 1323480 |
| effects of beta-endorphin on mu and delta opioid receptor-coupled g-protein activity: low-km gtpase studies. | human beta-endorphin 1-31 (beta-end) stimulated low-km gtpase activity in a concentration-dependent and saturable manner in membranes prepared from the delta opioid receptor-containing hybrid cell line ng108-15 and from the mu opioid receptor-enriched human neuroblastoma cell line sk-n-sh. naloxone and the delta-selective antagonist, ici 174,864, blocked the stimulation of the gtpase activity produced by beta-end in ng108-15 cell membranes, whereas only naloxone inhibited the beta-end-induced st ... | 1992 | 1328614 |
| adrenergic inhibition of insulin secretion involves pertussis toxin-sensitive and -insensitive mechanisms. | we studied the involvement of bordetella pertussis toxin (ptx)-sensitive g proteins in the inhibition by adrenaline of insulin secretion from the isolated rat pancreas. the -90% inhibition induced by adrenaline (0.05 microm) was partially abolished after in vivo ptx pretreatment. the residual inhibitory effect of adrenaline in ptx-pretreated rats was suppressed by the alpha 2-adrenoceptor antagonist, yohimbine, but was not modified by the alpha 1-adrenoceptor antagonist, prazosin. thus, the alph ... | 1992 | 1330603 |
| preparative separation of foreign antigens for highly efficient presentation to t cells in vitro. | a method is described for the separation and purification of proteins from complex mixtures of foreign antigens in a form suitable for stimulating t cells in vitro. the technique involves electrophoretic separation of proteins followed by elution, concentration and adsorption of the polypeptide subunits to latex microspheres. alternatively, where a specific antibody is available, proteins may be affinity-purified from a heterogeneous mixture of antigens, using antibody-coated latex microspheres. ... | 1992 | 1335464 |
| immune response in mice immunized with acidic antigenic fractions from trypanosoma cruzi cytosol. | the humoral and cellular immune responses as well as the resistance to infection with bloodstream forms of t. cruzi were studied in mice immunized with acidic antigenic fractions from parasite cytosol, f iii and f iv, plus bordetella pertussis as adjuvant. the immunization with f iii induced positive ith and dth responses to homologous antigens. in mice immunized with f iv, the ith was negative and four out of six animals presented positive dth reactions. in both groups of mice the analysis of i ... | 1992 | 1342100 |
| memory cell generation ablated by soluble protein antigen by means of effects on t- and b-lymphocyte compartments. | adult c57bl/6 mice were injected with 100 micrograms of soluble, freshly deaggregated human serum albumin (hsa) to produce partial immunologic tolerance. uninjected normal control (n) mice contain only approximately 100 b cells in their spleens with the capacity to (i) be activated in vitro into clonal proliferation by escherichia coli lipopolysaccharide plus interleukins 2, 4, and 5, (ii) form igg1 as well as igm antibody, and (iii) display specificity for hsa when only igg1 is allowed to score ... | 1992 | 1348366 |
| construction and analysis of bordetella pertussis mutants defective in the production of fimbriae. | although the role of fimbriae in bacterial disease has been well established, little is known about the function of bordetella pertussis fimbriae. to study this function, well-defined fimbrial mutants were constructed. b. pertussis harbours three fimbrial genes, fim2, fim3 and fimx, and strains were constructed in which one or more fimbrial genes were inactivated by means of gene replacement. analysis of these strains by means of immunoblotting suggested the presence of a fourth fimbrial gene, t ... | 1992 | 1350044 |
| role of carbohydrate recognition domains of pertussis toxin in adherence of bordetella pertussis to human macrophages. | pertussis toxin (pt) and filamentous hemagglutinin can each mediate the association of bordetella pertussis with human macrophages. adherence via filamentous hemagglutinin leads to integrin-mediated entry and survival of the bacteria within the human cell. we determined the contribution of pt to bacterial adherence to human macrophages. plating macrophages on wells coated with recombinant pt subunit 2 (s2) or s3 decreased pt-dependent bacterial binding by greater than 60%; s1, s4, and s5 were in ... | 1992 | 1353482 |
| common accessory genes for the bordetella pertussis filamentous hemagglutinin and fimbriae share sequence similarities with the papc and papd gene families. | the bordetella pertussis filamentous hemagglutinin (fha) is a major virulence factor responsible for attachment, one of the early events in bacterial pathogenesis. deletion of its structural gene, fhab, or a tn5 insertion in fhaa, downstream of fhab, resulted in a fha- and fimbriae- phenotype, although fhab and the fim genes are not linked. the fhab downstream region therefore most likely encodes accessory proteins required for the biosynthesis of fha and fimbriae, despite the lack of sequence s ... | 1992 | 1354611 |
| characterization of a metabotropic glutamate receptor: direct negative coupling to adenylyl cyclase and involvement of a pertussis toxin-sensitive g protein. | we have characterized a g-protein-coupled glutamate receptor in primary cultures of striatal neurons. glutamate, quisqualate, or trans-1-aminocyclopentane-1,3-dicarboxylate inhibited by 30-40% either forskolin-stimulated camp production in intact cells or forskolin plus vasoactive intestinal peptide-activated adenylyl cyclase assayed in neuronal membrane preparations. these inhibitory effects were suppressed after treatment of striatal neurons with bordetella pertussis toxin, suggesting the invo ... | 1992 | 1355603 |
| somatostatin inhibition of phosphoinositides turnover in isolated rat acinar pancreatic cells: interaction with bombesin. | the effects of somatostatin-14 and bombesin on [3h]inositol phosphate accumulation were studied in 24 h myo-[3h]inositol-prelabeled cultured rat acinar cells. bombesin, 10 nm, stimulated basal formation of phosphatidyl monophosphate (insp1), phosphatidyl 4,5-biphosphate (insp2) and inositol 1,4,5-triphosphate (insp3) by 128 +/- 5.2%, 147 +/- 10% and 155 +/- 5%, respectively. at 5 s, the ed50 value for insp3 stimulation was 0.70 +/- 0.2 nm. this stimulation was partly blocked (64 +/- 0.04% inhibi ... | 1992 | 1359613 |
| characterization of a bordetella pertussis fimbrial gene cluster which is located directly downstream of the filamentous haemagglutinin gene. | the biosynthesis of fimbriae is a complex process requiring multiple genes which are generally found clustered on the chromosome. in bordetella pertussis, only major fimbrial subunit genes have been identified, and no evidence has yet been found that they are located in a fimbrial gene cluster. to locate additional genes involved in the biosynthesis of b. pertussis fimbriae, we used tnphoa mutagenesis. a phoa+ mutant (designated b176) was isolated which was affected in the production of both ser ... | 1992 | 1360139 |
| immunochemical localization of a region of chaperonin-60 important for productive interaction with chaperonin-10. | an igg1 monoclonal antibody (mab 54g8) which binds to both bordetella pertussis chaperonin-60 (cpn60) and escherichia coli cpn60 (groel) was produced. mab 54g8 as well as fab fragments prepared from this antibody were found to abolish the ability of chaperonin-10 (cpn10, groes) to inhibit the atpase activity of both b. pertussis cpn60 and e. coli cpn60. electron microscopy was used to localize the binding site of the monoclonal antibody on the b. pertussis cpn60 molecule. in the absence of the a ... | 1992 | 1361184 |
| a call for a pertussis surveillance system. | pertussis is the 3rd highest vaccine preventable cause of death among children 5 years old. it is most frequent in children 2 years old and is fatal for 12 month old infants. people tend to confuse it with other respiratory infections. despite its importance and the fact that it is a reportable disease, health systems in the americas do not have an adequate surveillance system for pertussis. the number of reported cases in the americas fell 67% between 1980-1990 which corresponded with incre ... | 1992 | 12285225 |
| office diagnosis of pertussis. | 1992 | 22416193 | |
| acellular versus whole-cell pertussis vaccines. | 1992 | 22529731 | |
| production and release of tumor necrosis factor alfa, interleukin-1b and interleukin-6 by human mononuclear leukocytes stimulated with pertussis toxin. | pertussis toxin (pt) has been previously shown to affect a wide variety of immune responses and to cause lymphocyte proliferation. in this study, we examined the effect of pt on cultured human peripheral blood lymphocytes and monocytes with the regard to the capability of this toxin to stimulate the production and release of various cytokines. pt was found to induce the production and release of tumor necrosis factor alfa (tnf-alfa) and interleukin-6 (il-6) by both human lymphocytes and monocyte ... | 1993 | 8264421 |
| identification of bordetella avium using the polymerase chain reaction. | a dna fragment from bordetella pertussis, encoding the fim2 fimbrial subunit gene with adjacent sequences, was used as a probe for the detection of homologous sequences in chromosomal dna of bordetella avium. a 1.8 kb sa1i-psti fragment from the genome of b. avium, which hybridized with the probe, was isolated and sequenced. no fimbrial subunit gene was located on the b. avium dna fragment. two regions could be distinguished in the sequence of the fragment. region 1, which was 80% identical to t ... | 1993 | 8271920 |
| structural and physico-chemical characteristics of bordetella pertussis adenylate kinase, a tryptophan-containing enzyme. | the adk gene from the gram-negative pathogen bordetella pertussis was cloned by complementing the thermosensitive escherichia coli adk strain cr341t28. b. pertussis adenylate kinase is a 218-amino-acid protein that has high similarity with adenylate kinase from escherichia coli and hemophilus influenzae (57%). a distinct characteristic of enzyme from b. pertussis, not found in other bacterial adenylate kinases, is the presence of a tryptophan residue at position 185. although distant from the ca ... | 1993 | 8281944 |
| characterization of outbreak and vaccine strains of bordetella pertussis--quebec. | 1993 | 8298581 | |
| trypanosoma cruzi glutathione-binding proteins (tcgbp): protection induced by native proteins in an experimental model and analysis of the antibody response. | three trypanosoma cruzi glutathione-binding proteins (tcgbp) of 45, 30 and 25 kda presenting glutathione s transferase activity were characterized from t. cruzi epimastigotes. we show here that immunization of mice using tcgbp and complete freund's adjuvant (cfa) did not protect the animals against a challenge with bloodstream trypomastigotes. in contrast, immunization of mice using tcgbp in association with bordetella pertussis plus alum (bpai) resulted in greatly diminished parasitaemia and si ... | 1993 | 8303073 |
| the antiarrhythmic effect of ischaemic preconditioning in isolated rat heart involves a pertussis toxin sensitive mechanism. | the aims were to examine the effect of pretreatment with bordetella pertussis toxin on the antiarrhythmic effect of ischaemic preconditioning in order to determine the possible involvement of inhibitory g proteins in this phenomenon; and (2) to characterise the model used by varying the duration of a single preconditioning occlusion of the left coronary artery, the reperfusion time, and the duration of the subsequent prolonged coronary artery occlusion. | 1993 | 8324804 |
| pertussis toxin export requires accessory genes located downstream from the pertussis toxin operon. | pertussis toxin, a major virulence factor of bordetella pertussis, is an oligomeric protein composed of five different subunits that are exported individually to the periplasmic space by the signal peptide-dependent pathway. after assembly, the protein is exported from the periplasm to the extracellular compartment. we show that pertussis toxin secretion across the outer membrane requires the gene product of at least one gene (ptlc) that is located downstream from the pertussis toxin operon. the ... | 1993 | 8326857 |
| enhancement of histamine-induced vascular leakage by pertussis toxin in sjl/j mice but not balb/c mice. | pertussis toxin (ptx) from bordetella pertussis is known to enhance inflammatory responses which involve histamine and serotonin, including cell-mediated delayed-type hypersensitivity reactions. in this study we examined the effects of ptx on histamine-modulated microvascular responses. the actions of histamine on arteriole diameter and post-capillary leaky site formation in the cremaster muscle were measured intra-vitally in two inbred strains of mice (viz. balb/c and slj). in sjl mice the rate ... | 1993 | 8331165 |
| interleukin-1 is linked to the respiratory epithelial cytopathology of pertussis. | bordetella pertussis, the causative agent of whooping cough, releases a muramyl peptide known as tracheal cytotoxin (tct) that is responsible for destruction of ciliated epithelial cells lining the large airways. in vitro, tct has been shown to cause this specific pathology in human or hamster respiratory epithelium and to inhibit the proliferation of cultured hamster trachea epithelial cells. the diverse biological actions of muramyl peptides, including adjuvanticity, somnogenicity, and pyrogen ... | 1993 | 8335342 |
| effective immunization against bordetella pertussis respiratory infection in mice is dependent on induction of cell-mediated immunity. | a murine respiratory challenge model was used to examine the induction of cellular and humoral immune responses and their role in protection against bordetella pertussis following immunization or previous infection. spleen cells from mice convalescing from a b. pertussis infection exhibited extensive in vitro t-cell proliferation and secreted high levels of interleukin-2 (il-2) and gamma interferon but not il-4 or il-5, a cytokine profile typical of cd4+ th1 cells. serum from these mice had low ... | 1993 | 8335349 |
| clinical and laboratory diagnosis of pertussis in the regions of a large vaccine efficacy trial in germany. | as a support service for a pertussis vaccine efficacy trial, a central diagnostic laboratory was established. physicians in the geographic areas of the planned study were encouraged to send nasopharyngeal specimens from children and household contacts with cough illnesses whether or not the illnesses were typical of pertussis. from april, 1991, to february, 1992, 3629 specimens were received and in 601 instances (16.6%) bordetella pertussis was isolated. only 3.3% of patients with positive cultu ... | 1993 | 8345982 |
| differential regulation of bordetella pertussis virulence factors. | bordetella pertussis, the causative agent of whooping cough, regulates its virulence factors coordinately according to environmental parameters such as temperature and certain chemicals. a regulatory locus has been characterized which is essential for this regulation. this bvg locus codes for a two-component regulatory system composed of the sensor protein bvgs and the transcriptional activator protein bvga. it has been shown that the bvga and bvgs proteins are sufficient for the transcriptional ... | 1993 | 8347925 |
| bordetella pertussis adenylate cyclase: a toxin with multiple talents. | the adenylate cyclase toxin of bordetella pertussis is a secreted multifunctional protein, endowed with calmodulin-activated catalytic, haemolytic and cytotoxic activities. residues and domains involved in different functions have been localized and several permissive sites, able to accommodate insertion of peptides without impairing the different functions of the toxin, have been identified. a 400-bp region in the promoter upstream region of the cyaa gene, encoding the toxin, has been defined a ... | 1993 | 8347936 |
| major outbreak of pertussis in northern alberta, canada: analysis of discrepant direct fluorescent-antibody and culture results by using polymerase chain reaction methodology. | a major outbreak of 5,683 cases of pertussis occurred in northern alberta, canada, from december 1989 to january 1991. the outbreak highlighted a number of problems with current methods of pertussis diagnosis. in particular, an exceptionally high proportion of direct fluorescent-antibody (dfa)-positive, culture-negative specimens (88.4%) was identified. we took this opportunity to use polymerase chain reaction (pcr) methodology to examine whether the low culture rates were due to specimens conta ... | 1993 | 8349747 |
| protection of mice against respiratory bordetella pertussis infection by intranasal immunization with p.69 and fha. | intranasal immunization of adult female balb/c mice with the bordetella pertussis antigens fha or p.69, greatly enhanced their ability to clear b. pertussis from their lungs following aerosol challenge compared with ovalbumin-immunized controls. low numbers of lymphocytes secreting antibodies (igg, iga and igm) against the immunizing antigens could be isolated from the lungs of immunized mice. following aerosol challenge with b. pertussis there was a large increase in the numbers of fha or p.69- ... | 1993 | 8356847 |
| use of the polymerase chain reaction to detect bordetella pertussis in patients with mild or atypical symptoms of infection. | nasopharyngeal aspirates and nasopharyngeal swabs from 177 children exhibiting mild to severe clinical symptoms of whooping cough were tested by the polymerase chain reaction (pcr) and culture for the presence of bordetella pertussis. in the pcr analysis amplifications of samples prepared with and without dna extraction were compared. in 26% of samples prepared without dna extraction, the pcr was found to be inhibited, whereas no inhibition was detected after dna extraction. twelve percent (21/1 ... | 1993 | 8359168 |
| cyac-mediated activation is important not only for toxic but also for protective activities of bordetella pertussis adenylate cyclase-hemolysin. | bordetella pertussis adenylate cyclase-hemolysin (ac-hly), encoded by the cyaa gene, belongs to the rtx family of toxins with extensive glycine-rich repeats in the carboxy-terminal portion. ac-hly possesses both adenylate cyclase toxic and hemolytic activities that depend on a posttranslational modification mediated by the product of the cyac gene. an improved system for ac-hly synthesis and activation in escherichia coli was developed. the results show that with purified ac-hly (i) increased ex ... | 1993 | 8359880 |
| polymerase chain reaction assay for pertussis: simultaneous detection and discrimination of bordetella pertussis and bordetella parapertussis. | a polymerase chain reaction (pcr) assay which allows the simultaneous detection and discrimination of the two causative agents of pertussis, bordetella pertussis and bordetella parapertussis, was developed. primer pairs were based on insertion sequence elements is481 and is1001. is481 is specific for b. pertussis and is present in about 80 copies per cell, while is1001 is specific for b. parapertussis and is found in 20 copies per cell. an internal control was included in the pcr assay to monito ... | 1993 | 8370741 |
| lung infections in children. | airway infections in children is a considerably broad topic. this discussion focuses on several common nonbacterial causes of lower respiratory tract infection in children, including respiratory syncytial virus, mycoplasma pneumoniae, and chlamydia pneumoniae. in addition, the occurrence of two important bacterial causes of lower respiratory illness (bordetella pertussis and mycobacterium tuberculosis) is increasing. this review focuses on current information on the prophylaxis, treatment, and d ... | 1993 | 8374645 |
| ligand specificity of purified complement receptor type three (cd11b/cd18, alpha m beta 2, mac-1). indirect effects of an arg-gly-asp (rgd) sequence. | we have purified cr3 to homogeneity by affinity chromatography on c3bi-sepharose and elution with edta. c3bi-coated erythrocytes bound to this purified cr3, and binding was dependent on the concentration of both c3bi and cr3, as well as on temperature and the presence of divalent cations. moreover, binding could be blocked by mab against cr3 or c3bi and could be enhanced by the addition of integrin modulating factor-1. we used the purified cr3 to test whether several putative ligands of cr3 dire ... | 1993 | 8376780 |
| isolation and characterization of bordetella bronchiseptica mutants deficient in siderophore activity. | iron acquisition by the gram-negative pathogens bordetella bronchiseptica and bordetella pertussis is thought to occur by hydroxamate siderophore-mediated transport as well as an apparently siderophore-independent process by which host transferrins bind to bacterial surface receptors. we constructed b. bronchiseptica mutants deficient in siderophore activity by insertional mutagenesis with minitn5/lacz1. the mutants could be placed into four distinct complementation groups, as determined from cr ... | 1993 | 8381782 |
| characterization of adenylate cyclase toxin from a mutant of bordetella pertussis defective in the activator gene, cyac. | bordetella pertussis adenylate cyclase (ac) toxin has the abilities to 1) enter target cells where it catalyzes cyclic amp production and 2) lyse sheep erythrocytes, and these abilities require post-translational modification by the product of an accessory gene cyac (barry, e. m., weiss, a. a., ehrmann, e. e., gray, m. c., hewlett, e. l., and goodwin, m. st. m. (1991) j. bacteriol. 173, 720-726). in the present study, ac toxin has been purified from an organism with a mutation in cyac, bpde386, ... | 1993 | 8385122 |
| shapes of cells spreading on fibronectin: measurement of the stellation of bhk21 cells induced by raising cyclic amp, and of its reversal by serum and lysophosphatidic acid. | in common with many other animal cells in culture, bhk21, cho and nih-3t3 cells adopt bizarre stellate or arborized shapes when exposed, in the absence of serum, to agents which increase cytoplasmic cyclic amp (camp). dibutyryl camp, 3-isobutyl-1-methylxanthine, 5'-deoxy-5'-methylthioadenosine, cholera toxin and the invasive adenylate cyclase from bordetella pertussis all induce similar shapes. time lapse video recording of bhk21 cells spreading on fibronectin shows that stellate shapes are gene ... | 1993 | 8389376 |
| the adenylate cyclase toxin contributes to the survival of bordetella pertussis within human macrophages. | the adenylate cyclase toxin (act) of bordetella pertussis has been shown to penetrate eucaryotic cells and produce a rapid elevation in intracellular camp which leads to altered cell function. recent studies have demonstrated an intracellular state for the bacteria within professional and non-professional phagocytes. a virulent strain was compared to two act defective strains to determine if this toxin contributes to intracellular survival within human macrophages. when challenged by 10(6) macro ... | 1993 | 8392135 |
| dna topology affects transcriptional regulation of the pertussis toxin gene of bordetella pertussis in escherichia coli and in vitro. | the bvg locus of bordetella pertussis encodes an environmentally inducible operon essential for the expression of virulence genes. we show that in escherichia coli, the ptox promoter cloned in cis of the bvg locus is activated and environmentally regulated. cotransformation of e. coli with the bvg locus cloned in a low-copy-number plasmid and with the ptox promoter cloned in a high-copy-number plasmid can give rise to two different results. if the ptox promoter is cloned in the pgem-3 vector, tr ... | 1993 | 8393006 |
| [persistent cough caused by bordetella pertussis. study of 6 cases]. | pertussis is currently an infrequent illness in spain, since dtp vaccine was introduced. immunity acquired after immunization is, nevertheless, lower than immunity acquired after the clinical disease, and decreases steadily. after the adolescence, serum levels of antibodies against b. pertussis may not be enough, leading to atypical infections in other wise immunized patients. | 1993 | 8399476 |
| structural features involved in the mitogenic activity of bordetella pertussis lipopolysaccharides for spleen cells of c3h/hej mice. | spleen cells from the c3h/hej mouse strain cannot be stimulated by many smooth-type lipopolysaccharides (lpss), and by the main biologically-active region (lipid a) of these molecules. the genetic origin of this defect (expression of the mutant allele lpsd at the chromosome 4 locus) was established over 20 years ago, but its biochemical nature has remained undefined. several investigators have noted, however, that some particular lpss can bypass this defect, and stimulate the proliferation of c3 ... | 1993 | 8401423 |
| [nucleotide sequence and properties of an inverted repeating element of a bordetella pertussis chromosome]. | the repeated sequence from bordetella pertussis chromosome was cloned using the method described by ohtsubo. the sequences of the characterized b. pertussis chromosome are homologous to the previously described sequences and analogous in the structure to the already known is elements. the parental recombinant plasmids containing the rs were unstable and segregated to the plasmids of different structure. the segregants' structure is characterized in this paper. it is shown in our study that the r ... | 1993 | 8405971 |
| bordetella pertussis induces apoptosis in macrophages: role of adenylate cyclase-hemolysin. | bordetella pertussis, the causative agent of whooping cough, has been shown recently to enter and survive in epithelial cells and macrophages in vitro. in the present study, we show that b. pertussis is cytotoxic for j774a.1 cells, a monocyte-macrophage cell line, and for murine alveolar macrophages. we demonstrate that cell cytotoxicity mediated by b. pertussis occurred through apoptosis, as shown by changes in nuclear morphology and by host cell dna fragmentation. parental strains and a mutant ... | 1993 | 8406793 |
| pertussis toxin-induced alterations of murine hepatic drug metabolism following administration of diphtheria and tetanus toxoids and pertussis vaccine adsorbed. | administration of pertussis toxin (pt) in combination with diphtheria and tetanus toxoids adsorbed (dt vaccine) or with acellular pertussis vaccine adsorbed and diphtheria and tetanus toxoids (apdt) elicits dose- and time-dependent alterations in hepatic drug metabolism in mice. cytochrome p-450 (p-450) levels were inhibited more than 50% at 7 days following a single injection of pt mixed with either vaccine. when combined with dt vaccine, 125 ng of pt was required to produce this effect, while ... | 1993 | 8406812 |
| a phase variant of bordetella pertussis with a mutation in a new locus involved in the regulation of pertussis toxin and adenylate cyclase toxin expression. | a novel nonhemolytic phase variant of bordetella pertussis was characterized. this strain is strongly impaired in the transcription of the pertussis and adenylate cyclase toxins, whereas other known virulence-related factors such as the filamentous hemagglutinin, the fimbriae, and the outer membrane protein pertactin are expressed and regulated normally. complementation and allelic exchange experiments demonstrated that the mutation is localized neither in the bvg locus involved in virulence reg ... | 1993 | 8407844 |
| virulence of a bordetella pertussis strain expressing a mutant adenylyl cyclase with decreased calmodulin affinity. | bordetella pertussis, the pathogen responsible for whooping cough, produces a toxic calmodulin-sensitive adenylyl cyclase which enters animal cells and increases intracellular camp. a point mutant of b. pertussis with abolished adenylyl cyclase catalytic activity was over 1000-fold less pathogenic to newborn mice than wild-type bacteria, demonstrating the importance of the adenylyl cyclase for b. pertussis virulence (gross et al.). the b. pertussis adenylyl cyclase is highly sensitive to calmodu ... | 1993 | 8412621 |
| orientation of porin channels in the outer membrane of bordetella pertussis. | we have examined the surface topography and channel connectivity of a naturally crystalline porin that is known to be functional, and whose structure has not been perturbed by detergent extraction. a three-dimensional density map, calculated from two independent tilt series of negatively stained cell envelopes, reveals three separate channels per trimer on one side (the 'smooth' side), and a single common opening at the other ('rough') side. this arrangement is consistent with the molecular stru ... | 1993 | 8412696 |
| haemophilus influenzae type b polysaccharide-tetanus protein conjugate vaccine does not depress serologic responses to diphtheria, tetanus or pertussis antigens when coadministered in the same syringe with diphtheria-tetanus-pertussis vaccine at two, four and six months of age. | the safety and immunogenicity of a vaccine against haemophilus influenzae type b consisting of purified polyribosylribitol phosphate conjugated to tetanus toxoid (prp-t) were evaluated in 277 chilean infants who were randomly assigned to one of three treatment groups: group a, prp-t mixed with diphtheria-tetanus-pertussis (dtp) vaccine in a single syringe and given as a single inoculation in one arm and placebo in the other arm; group b, prp-t given in one arm and dtp in the other arm; group c, ... | 1993 | 8414775 |
| adhesion of bordetella pertussis to eukaryotic cells requires a time-dependent export and maturation of filamentous hemagglutinin. | bordetella pertussis, the human pathogen of whooping cough, when grown at 22 degrees c is nonvirulent and unable to bind eukaryotic cells. in response to a temperature shift to 37 degrees c, the bacterium acquires the ability to bind eukaryotic cells in a time-dependent fashion. by studying in vitro the temperature-induced transition, from the nonvirulent to the virulent state, we found that binding to cho cells is mediated by the arg-gly-asp-containing domain of filamentous hemagglutinin (fha), ... | 1993 | 8415678 |
| rapid immunodot technique for identifying bordetella pertussis. | we developed and evaluated a rapid test with monoclonal antibodies to identify cultures of bordetella pertussis. samples of 5 microliters of cells suspended in formalin-saline were dried onto a nitrocellulose disk. the disk was placed in a filtration device, and 5-microliters volumes of murine monoclonal antibody directed against b. pertussis lipooligosaccharide and peroxidase conjugate were added consecutively, with washing after each addition. the disk was removed and immersed in peroxidase su ... | 1993 | 8417027 |
| comparison of nasopharyngeal aspirates with swabs for culture of bordetella pertussis. | nasopharyngeal samples were collected from 117 children by aspiration from one nostril and by swab from the other one and cultured for bordetella pertussis. among 33 culture-positive specimens, there were 30 positive aspirates and 26 positive swab specimens. aspirates are easily divided and saved for investigations with other assays which may further improve diagnostic sensitivity. as the aspiration technique was also preferred by nurses and parents, it was recommended and chosen for a planned p ... | 1993 | 8417032 |
| repressor binding to a regulatory site in the dna coding sequence is sufficient to confer transcriptional regulation of the vir-repressed genes (vrg genes) in bordetella pertussis. | five tnphoa fusions to vir-repressed genes (vrg genes) have been identified in the respiratory pathogen bordetella pertussis. a comparison of vrg dna sequences suggests a consensus dna element within the coding regions of four of five vrg genes. to determine the role of this dna sequence in vrg regulation, a nucleotide substitution mutation in the conserved region of vrg-6 was isolated. this mutant showed constitutively high levels of expression in the absence of antigenic modulators, mgso4 and ... | 1993 | 8419298 |
| cooperative phenomena in binding and activation of bordetella pertussis adenylate cyclase by calmodulin. | the catalytic domain of bordetella pertussis adenylate cyclase located within the first 400 amino acids of the protein can be cleaved by trypsin in two subdomains (t25 and t18) corresponding to atp-(t25) and calmodulin (cam)-(t18) binding sites. reassociation of subdomains by cam is a cooperative process, which is a unique case among cam-activated enzymes. to understand better the molecular basis of this phenomenon, we used several approaches such as partial deletions of the adenylate cyclase ge ... | 1993 | 8420945 |
| characterization of a synthetic calmodulin-binding peptide derived from bacillus anthracis adenylate cyclase. | a 34-amino acid peptide corresponding to residues 532-565 of bacillus anthracis adenylate cyclase (p532-565), a calmodulin (cam)-activated enzyme, was synthesized by solid phase method. although not homologous to any known cam binding sequence, p532-565 exhibits molecular features characteristic of this class of peptides: a higher proportion of basic and hydrophobic residues, segregated onto the two faces of the alpha-helical structure. fluorescence measurements and gel retardation analysis show ... | 1993 | 8420946 |
| efficacy of acellular pertussis vaccine in young infants. | a prospective study of a pertussis outbreak in a residential facility was done. among 19 residents aged < or = 2 years, 10 children were unimmunized and 9 were immunized with acellular pertussis vaccines. of the 10 unimmunized children, 7 acquired laboratory-confirmed pertussis (four-fold titer rise, positive culture, or both); of these, 6 developed typical symptoms. eight of the 9 immunized children acquired laboratory-confirmed infections, and 1 of these developed typical symptoms. no differen ... | 1993 | 8421187 |
| cell-mediated immunity to bordetella pertussis: role of th1 cells in bacterial clearance in a murine respiratory infection model. | a murine respiratory infection model was used to study the mechanism of protective immunity to bordetella pertussis. we found that nude mice, which are deficient in t cells, developed a persistent infection and failed to clear the bacteria after aerosol inoculation. in contrast, normal adult nonimmune mice cleared a respiratory infection approximately 35 days after challenge. before bacterial clearance, antipertussis antibody levels in serum were low or undetectable, whereas consistent antigen-s ... | 1993 | 8423070 |
| bordetella pertussis and bordetella parapertussis: two immunologically distinct species. | bordetella pertussis and bordetella parapertussis are closely related species. both are responsible for outbreaks of whooping cough in humans and produce similar virulence factors, with the exception of pertussis toxin, specific to b. pertussis. current pertussis whole-cell vaccine will soon be replaced by acellular vaccines containing major adhesins (filamentous hemagglutinin and pertactin) and major toxin (pertussis toxin). all of these factors are antigens that stimulate a protective immune r ... | 1993 | 8423077 |
| antibody responses in the serum and respiratory tract of mice following oral vaccination with liposomes coated with filamentous hemagglutinin and pertussis toxoid. | mice were orally vaccinated with liposomes coated with filamentous hemagglutinin (fha) and detoxified pertussis toxin (pt) of bordetella pertussis. fha- and pt-specific immunoglobulin g (igg) was detected in serum, and both igg and iga were detected in lung washes following the immunization. antibody responses in mice immunized with liposomes coated with fha and pt were significantly higher than those in mice immunized with free fha and pt, which demonstrated the adjuvanticity of the liposome ca ... | 1993 | 8423087 |
| neisseria meningitidis produces iron-regulated proteins related to the rtx family of exoproteins. | a monoclonal antibody (a4.85) which reacted with fe-regulated proteins of neisseria meningitidis, was used to isolate a lambda gt11 clone from n. meningitidis fam20. chromosomal fragments flanking the fragment expressing the a4.85 epitope were cloned, and their dna sequences revealed a 3,345-bp open reading frame predicting a 122-kda protein. this gene was named frpa (fe-regulated protein). a computer similarity search of genbank revealed high levels of similarity to members of the rtx family of ... | 1993 | 8423153 |
| identification of bordetella pertussis in nasopharyngeal swabs by pcr amplification of a region of the adenylate cyclase gene. | the polymerase chain reaction (pcr) was used to amplify a 522-bp region of the adenylate cyclase toxin (cyaa) gene of bordetella pertussis. as few as 100 cfu from a suspension of b. pertussis could be detected by this procedure when the amplified pcr product was detected by ethidium bromide staining of agarose gels. however, simulated clinical specimens, prepared from swabs impregnated with known numbers of b. pertussis cells, only yielded a positive reaction with > or = 10(4) cfu. hybridisation ... | 1993 | 8429539 |
| pertussis is rare in human immunodeficiency virus disease. | many adults are susceptible to pertussis, and bordetella pertussis has been isolated from five patients with hiv disease. the prevalence of b. pertussis in 60 hiv-infected adults with nasopharyngeal (np) swab cultures were studied and questionnaires were used that assessed hiv-related risk behaviors and disease status, immunization history, and symptoms of respiratory disease. although 72% had cough and 33% had cough for > 14 days, no nasopharyngeal (np) swab cultures were positive for bordetell ... | 1993 | 8430967 |
| characterization of environmental regulators of bordetella pertussis. | bordetella pertussis suppresses transcription of its virulence genes in response to specific environmental conditions, a response called modulation. the organism responds to high concentrations of so4 and cio4 ions, nicotinic acid, and nicotinic acid analogs in vitro; however, the in vivo modulator has not been identified. we investigated which chemical structures of the nicotinic acid molecule are important for modulation by testing various analogs for their ability to modulate. the ring nitrog ... | 1993 | 8432601 |
| inhibition of bordetella pertussis filamentous hemagglutinin-mediated cell adherence with monoclonal antibodies. | filamentous hemagglutinin (fha), a 220-kda protein located on the surface of bordetella pertussis, is one of the major cell adhesins of this bacterium. we have produced three hybridoma cell lines that express monoclonal antibodies (mabs) against fha: x3c, x3e and x4b. the anti-fha mabs x3c and x3e reacted with 220-kda and 98-kda fha protein bands on western blots. the mab x4b, which reacted with fha in elisa, did not bind to fha in a western blot assay. all three mabs seemed to be directed to th ... | 1993 | 8440465 |
| interferon-gamma levels in serum and bronchoalveolar lavage fluid of mice infected with bordetella pertussis. | the adherence of bordetella pertussis to respiratory cilia and its survival in neutrophils and macrophages is crucial to the pathogenesis of whooping cough. to investigate the role of endogenous interferon (ifn)-gamma in acute infection, levels of ifn-gamma in bronchoalveolar lavage (bal) fluid of mice infected intranasally with b. pertussis were determined. since pertussis toxin is released during infection by b. pertussis either locally or systemically, serum levels of ifn-gamma in mice inject ... | 1993 | 8440945 |