Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| differential uptake and killing potential of campylobacter jejuni by human peripheral monocytes/macrophages. | the ability of campylobacter jejuni to survive in monocytes after phagocytic uptake was tested in a new in vitro model using adherent macrophages derived from human peripheral monocytes. the cells were stimulated with cytokines before use to ensure full phagocytic and killing activity. the kinetics of uptake and killing of bacteria was followed for 72 h with 16 strains, including stool and blood isolates and laboratory adapted strains. significant bacterial strain differences were not observed, ... | 1997 | 9403842 |
| construction of a helicobacter pylori-escherichia coli shuttle vector for gene transfer in helicobacter pylori. | in this study, a helicobacter pylori-escherichia coli shuttle vector was constructed for transferring dna into h. pylori. the smallest cryptic plasmid (1.2 kb), php489, among those harbored by 77 h. pylori isolates was selected as a base replicon for constructing vectors. hindiii-digested php489 was ligated with a kanamycin resistance gene [aph(3')-iii], which originated from campylobacter jejuni, to produce the recombinant plasmid php489k. php489k was efficiently transformed into and stably mai ... | 1997 | 9406406 |
| analysis of distribution of campylobacter jejuni and campylobacter coli in broilers by using restriction fragment length polymorphism of flagellin gene. | the incidence of campylobacter jejuni and campylobacter coli in broiler farms was 33.9% (19/56). c. jejuni-positive flocks accounted for 20.0% (17/85) and c. coli-positive ones was 4.7% (4/85). there were 14 patterns (fla type) of restriction fragment length polymorphism (rflp) of flagellin a gene among these 22 strains of c. jejuni and c. coli including the standard strain c. jejuni atcc 33560. different fla types of campylobacter were isolated from broilers in different growing cycles on the s ... | 1997 | 9409516 |
| [the drug susceptibility pattern of the presumed etiologic agents of infectious enteritis including verotoxin-producing escherichia coli o-157]. | the drug susceptibility patterns were investigated for verotoxin-producing escherichia coli (vtec) including o-157, salmonella spp., vibrio parahaemolyticus and campylobacter jejuni subsp. jejuni that were obtained in and after july 1996. the results are summarized as follows; 1. we found highly resistant strains of vtec to tetracycline (tc) and ampicillin (abpc). minimum inhibitory concentrations (mic) of some of the drugs against vtec in an aerobic condition were significantly different from t ... | 1997 | 9412873 |
| campylobacter jejuni in broiler chickens: colonization and humoral immunity following oral vaccination and experimental infection. | a formalin inactivated, campylobacter jejuni whole cell vaccine, either with or without escherichia coli heat labile toxin (lt) as a mucosal adjuvant, was administered orally to broiler chickens. three vaccine trials were performed, differing in the number of vaccinations, and time of administration, as well as the inclusion and dose of lt. the overall reductions of c. jejuni colonization in the vaccinated chickens ranged from 16 to 93% compared with non-vaccinated controls. enhanced levels of a ... | 1997 | 9413103 |
| human igm paraproteins demonstrate shared reactivity between campylobacter jejuni lipopolysaccharides and human peripheral nerve disialylated gangliosides. | igm paraproteins from patients with canomad (chronic ataxic neuropathy, ophthalmoplegia, m-protein, agglutination, anti-disialosyl antibodies) react with neuac(alpha 2-8)neuac epitopes on a wide range of gangliosides including gq1b, gt1a, gd1b and gd3. the tissue distribution of reactive antigens in human peripheral nerve has not been addressed in detail. in addition, the origin of these antibodies is unknown. here we report that purified anti-disialosyl paraproteins from two affected patients b ... | 1997 | 9413256 |
| the prevalence of campylobacters and arcobacters in broiler chickens. | chicken carcasses from a supermarket and from a poultry abattoir were examined using methods designed to isolate as many strains of campylobacters and related organisms as possible. strains of arcobacter, but no campylobacters, were isolated from every carcass after enrichment. campylobacter jejuni subsp. jejuni was isolated from all carcasses examined by direct plating and other campylobacter-like strains were isolated from nine out of 15 abattoir carcasses by direct plating but not after enric ... | 1997 | 9418023 |
| detection of campylobacter jejuni in food and poultry viscera using immunomagnetic separation and microtitre hybridization. | thermophillic campylobacter and camp. jejuni were detected from samples of chicken liver, gall bladder, muscle and contaminated milk and chicken meat after an enrichment step by using immunomagnetic capture of cells with monoclonal antibody against a specific outer membrane protein of thermophilic campylobacter. the detection of captured cells was achieved using two different hybridization methods. in one of the methods, the captured cells were lysed by guanidine isothiocyanate and the 23s rrna ... | 1997 | 9418026 |
| cloning and characterization of the lytb gene of campylobacter jejuni. | lytb of escherichia coli is an essential gene involved in penicillin tolerance and the stringent response. the lytb gene of campylobacter jejuni was cloned and characterized. it could complement a temperature-sensitive e. coli lytb mutant. the c. jejuni lytb gene encodes a protein of 277 amino acids that has 34, 36 and 40% amino acid identity with the lytb proteins of e. coli, haemophilus influenzae, and synechocystis sp. pcc6803, respectively. the lytb gene is situated between the aroa gene and ... | 1997 | 9418246 |
| comparison of the antibacterial activities of the quinolones bay 12-8039, gatifloxacin (am 1155), trovafloxacin, clinafloxacin, levofloxacin and ciprofloxacin. | the in-vitro activities of the quinolones bay 12-8039, gatifloxacin (am 1155), trovafloxacin, clinafloxacin, levofloxacin and ciprofloxacin were compared. gram-positive cocci were most susceptible to bay 12-8039, clinafloxacin and trovafloxacin; enterobacteriaceae and fastidious organisms were most susceptible to clinafloxacin [corrected]; pseudomonas spp. were most susceptible to clinafloxacin and ciprofloxacin; anaerobes, helicobacter pylori and campylobacter jejuni were most susceptible to ga ... | 1997 | 9421311 |
| restriction fragment length polymorphism analysis shows that the hippuricase gene of campylobacter jejuni is highly conserved. | a 1151-bp amplicon containing the hippuricase (hipo) gene was obtained from 118 strains of campylobacter jejuni and double-digested with alui and ddei to give five different pcr-rflp patterns. most strains had the six-banded profile predicted from sequence data. lack of polymorphisms within the hipo gene indicated it was highly conserved amongst strains of camp.jejuni, and the rflp analysis provided only low discrimination as an epidemiological typing method. detection of hipo by pcr provided a ... | 1997 | 9424104 |
| [enzymatic activities of campylobacter jejuni, c. coli, and c. lari]. | susceptibility to nalidixic acid has been considered for a long time as an important test in the identification of the different species of the genus campylobacter. however, due to the increasing development of resistance new laboratory tests are needed to carry out an accurate identification to the species level in combination with other typing schemes. we have evaluated the enzymatic activity of 180 campylobacter spp. strains isolated from clinical specimens performing the api zym system (biom ... | 1997 | 9424627 |
| double staining (ctc-dapi) for detection and enumeration of viable but non-culturable campylobacter jejuni cells. | direct microscopic enumeration of viable campylobacter jejuni cells (ie, respiring bacteria) were performed in both culturable and non-culturable states. five different c jejuni strains were used, including a reference strain, atcc 33291. cells from all five strains were incubated alone with 5-cyano-2,3-ditolyl tetrazolium chloride (ctc), a redox dye. it was reduced by an electron transport chain to an insoluble red fluorescent ctc formazan salt, which accumulated intracellularly. the presence o ... | 1997 | 9428148 |
| short report: impact of traveler's diarrhea on united states troops deployed to thailand. | among a united states military unit of 170 personnel deployed to utapao, thailand for a three-week training exercise, 40% experienced diarrheal disease, and 12% sought medical treatment for diarrhea. most illness clustered within the first two weeks of arrival and individuals were ill an average of 3.6 days. fifty-five percent of cases lost two days of work and 15% required treatment with intravenous fluids. bacterial pathogens were recovered from 38% of 16 stools submitted, with campylobacter j ... | 1997 | 9430530 |
| campylobacter enteritis in children in an urban community. | to determine the prevalence of campylobacter species as aetiologic agents of diarrhoea and its antimicrobial susceptibility pattern. | 1997 | 9431745 |
| genotype analysis of human blood isolates of campylobacter jejuni in england and wales. | genomic profiles were obtained for 76 strains of campylobacter jejuni isolated from bacteraemic patients in england and wales over the period 1981-94. genotyping was performed by restriction fragment length polymorphism (rflp) analysis using a random cloned dna probe, and by ribotyping with a pcr-generated c. jejuni 16s ribosomal dna probe. phenotypic characterization was achieved by heat-stable (hs) and heat-labile (hl) serogrouping, and preston phagetyping and biotyping. the blood isolates wer ... | 1997 | 9129583 |
| the serotype distribution of campylobacter jejuni and c. coli isolated from patients with diarrhoea and controls at tikur anbassa hospital, addis ababa, ethiopia. | sixty-eight isolates of campylobacter jejuni and c. coli isolated from patients with diarrhoea (n = 630) and controls (n = 220) at tikur anbassa hospital, addis ababa, ethiopia were serotyped on the basis of the heat-labile (hl) and the heat-stable (hs) antigens, by using 16 and 34 antisera, respectively, for the two methods. with the antisera against heat labile antigens, 89.3% of the c. jejuni and 75% of the c. coli were typable. the hl serotypes 1, 2, 3, 4, 5, 6 and 7 were the most common amo ... | 1997 | 9129584 |
| cloning of an outer membrane protein gene from campylobacter jejuni. | an antigen in the outer membrane protein (omp) fraction of campylobacter jejuni was identified and characterized. western blot analysis demonstrated antigenic differences in this protein between two congenic c. jejuni strains. strain a74/c, which colonizes chickens, expressed the antigen at 34 kda, while strain a74/o, which poorly colonizes chickens, expressed the antigen at 32 and 34 kda. a genomic library was constructed in lambdagt11 with dna from a74/o and screened with antibody raised again ... | 1997 | 9142743 |
| in vitro permeation of human chorioamniotic membranes by campylobacter jejuni. | in vitro penetration of human chorioamniotic membranes by campylobacter jejuni was investigated by an organ culture model. membrane permeation was detected by an immunoperoxidase technique and viable bacterial counts of membrane homogenates. human clinical isolates of c. jejuni inoculated on the maternal side of the membranes penetrated to the fetal side suggesting that chorioamniotic membranes constituted a weak barrier against campylobacter infection. chicken fecal isolates did not penetrate c ... | 1997 | 9144913 |
| [a case of guillain-barré syndrome after the travel in southeast asia--isolation of campylobacter jejuni pen 5 serotype]. | a 57-year-old man, while on travel in malaysia, suffered from diarrhea after he ate fruits. he developed limbs weakness without sensory disturbance after his return to japan. serum from the patient had high igg anti-gm1 antibody titer. campylobacter jejuni was isolated from his stool. the serotype belonged to pen 5. the patient received double-filtration plasmapheresis 7 times during from days 6 to 17. muscle strength began to recover gradually on day 10, and returned to normal 5 months after th ... | 1997 | 9146072 |
| campylobacter spp. and salmonella spp. in black-headed gulls (larus ridibundus). | cloacal examination of 41 juvenile black-headed gulls (larus ridibundus) by cultivation demonstrated campylobacter jejuni in 26 (63%) and salmonella typhimurium in 21 (51%) of them. all the bird samples were collected in a breeding colony near the town hodonin, south moravia, czech republic in 1996. twenty six campylobacter isolates were tested for antibiotic and drug sensitivity: all were resistant to at least three agents (penicillin, tetracyclin and sulfomethoxazol-trimethoprim) while all wer ... | 1997 | 9151001 |
| [analysis of the swimming pattern and the velocity of bacteria using video tracking method]. | the swimming patterns and the velocities of several flagellated bacteria were measured by a computer assisted video tracking method. the moving path of the individual bacterium revealed that the bacterium frequently changed its swimming direction and velocity. the velocity among bacterial strains varies widely. in low viscous environment. campylobacter jejuni has characteristic swimming pattern with frequent changes in their swimming direction. as the viscosity increase, c. jejuni increases its ... | 1997 | 9154715 |
| the campylobacter jejuni porin trimers pack into different lattice types when reconstituted in the presence of lipid. | purified major outer membrane protein of campylobacter jejuni exhibited different classes of molecules by sds/page and immunoblotting. a high-molecular-mass product (120-140 kda) was observed under mild conditions of solubilization, a folded monomeric form of 35 kda was seen when treated at high sds concentrations and finally, a single band around 45 kda occurred when the sample was heated to 96 degrees c [bolla, j. m., loret, e., zalewski. m. & pages, j. m. (1995) j. bacteriol. 177, 4266-4271]. ... | 1997 | 9119026 |
| close association of guillain-barré syndrome with antibodies to minor monosialogangliosides gm1b and gm1 alpha. | cumulative evidence supports the theory that anti-ganglioside antibodies function in the development of guillain-barré syndrome (gbs). some patients have developed gbs after the administration of monosialoganglioside extracted from bovine brain. to clarify the pathogenesis of gbs associated with and without administration of the monosialoganglioside fraction, we investigated serum antibodies to the minor monosialogangliosides gm1b and gm1 alpha in patients with gbs and in control patients. gm1b ... | 1997 | 9119976 |
| a mixed foodborne outbreak with salmonella heidelberg and campylobacter jejuni in a nursing home. | to investigate a mixed salmonella heidelberg and campylobacter jejuni foodborne outbreak in a nursing home. | 1997 | 9120239 |
| [guillain-barré syndrome and fisher syndrome]. | guillain-barré syndrome is an acute inflammatory polyneuropathy that usually follows infections. fisher syndrome is a variant of guillain-barré syndrome, the triads of which are ophthalmoplegia, ataxia and areflexia. although the agents for the preceding infections cannot be determined in many cases, those viruses such as cytomegalovirus, epstein-barr virus, and hiv as well as mycoplasma and bacteria including campylobacter jejuni have been reported. the pathogenetic mechanisms may be immune-med ... | 1997 | 9103898 |
| acute motor axonal neuropathy with high titer igg and iga anti-gd1a antibodies following campylobacter enteritis. | we describe the first two european cases of acute axonal motor neuropathy with both igg and iga anti-gd1a antibodies following campylobacter enteritis. both patients acutely developed severe weakness without sensory involvement, had antibodies to campylobacter jejuni and polyclonal igg and iga titers > or = 12,800 to gd1a at onset, which decreased during follow-up. serial electrophysiologic studies showed: 1, normal or only slightly slowed motor conductions; 2, evidence of a progressive loss of ... | 1997 | 9106127 |
| a diffusible cytotoxin of haemophilus ducreyi. | little is known about the virulence mechanisms employed by haemophilus ducreyi in the production of genital ulcers. this gram-negative bacterium previously has been shown to produce a soluble cytotoxic activity that kills hela and hep-2 cells. we have now identified a cluster of three h. ducreyi genes that encode this cytotoxic activity. the predicted proteins encoded by these genes are most similar to the products of the escherichia coli cdtabc genes that comprise the cytolethal distending toxi ... | 1997 | 9108104 |
| history of macrolide use in pediatrics. | erythromycin, the prototypical macrolide, has been widely used since the 1950s in the management of pediatric infections. erythromycin is the drug of choice for infants and children with legionnaire's disease, pertussis, diphtheria, lower respiratory tract infections caused by mycoplasma pneumoniae, chlamydia pneumoniae and chlamydia trachomatis and enteritis caused by campylobacter jejuni. it is also indicated for treatment of syphilis; for streptococcal, staphylococcal and pneumococcal infecti ... | 1997 | 9109154 |
| igm anti-gm1 antibodies in the guillain-barré syndrome: a serological predictor of the clinical course. | it has been suggested that antibodies against gm1 are involved in the pathogenesis of the guillain-barré syndrome (gbs). recently, we have developed a standardized elisa assay for anti-gm1 antibodies of igm type well-suited for longitudinal patient studies. the relationship between serum antibodies against gm1 and campylobacter jejuni was investigated in patients with gbs and in patients with c. jejuni infection. patients with a short-lasting anti-gm1 elevation had a fast recovery, whereas patie ... | 1997 | 9003245 |
| application of the mast resistotyping scheme to campylobacter jejuni and c. coli. | the mast resistotyping scheme was assessed with 228 strains of campylobacter jejuni and c. coli from enteric infections in man and from a diverse selection of other sources (livestock, chickens and river water). most (153 of 158) c. jejuni examined were of the three most common penner (heat stable, hs) serotypes, hs1, hs2 and hs4 complex. fourteen resistotypes were identified in the 158 strains of c. jejuni and 16 in the 70 isolates of c. coli. the predominant codes were 00 (44% of c. jejuni; 33 ... | 1997 | 9003743 |
| acute diarrhea in under five-year-old children admitted to king mongkut prachomklao hospital, phetchaburi province. | a prospective epidemiological and clinical study of acute diarrhea in children under 5 years old was done at king mongkut prachomklao hospital in order to provide baseline data for health officers to make a strategic plan to reduce the diarrheal mortality and morbidity, which is one of the mid-decade goals for children. there were 105 cases of acute diarrhea patients admitted to the pediatric ward between may 1995 and april 1996. seventy-six per cent of them were in the younger age group (> 1 mo ... | 1997 | 9078814 |
| campylobacter-host cell interactions. | the enteric pathogens campylobacter jejuni and campylobacter coli are a major cause of infectious diarrhoea. their ability to adhere to human epithelial cells is ubiquitous and their propensity to invade cells is also well documented and requires motility and de novo protein synthesis, as well as several host factors. the molecular basis of the interaction between campylobacters and host cells is only beginning to be elucidate. the characteristics of this interaction promise to be interesting an ... | 1997 | 9080607 |
| outbreak of campylobacter enteritis in a residential school associated with bird pecked bottle tops. | an outbreak of gastroenteritis caused by campylobacter infection was identified in may 1995 in a residential school in gloucestershire for children with special needs. eight primary and four secondary cases were identified, mostly confined to one house in the school; faecal specimens from three cases grew campylobacter jejuni. a retrospective cohort study showed that drinking pasteurised milk from bottles with damaged tops was associated with illness in primary cases (p = 0.01). bird pecking of ... | 1997 | 9080727 |
| cranial polyneuropathy with elevated serum antiganglioside antibody. | we report a 6-year-old girl with cranial polyneuropathy with elevated serum levels of antiganglioside antibodies. she manifested herpetic vesicles around the right upper eyelid and mouth without antecedent infection. she developed facial asymmetry and double vision 5 days after the first appearance of the vesicles. neurological examination on admission disclosed palsies of the bilateral sixth and twelfth cranial nerves and right third and seventh cranial nerves, but limb muscle weakness, ataxia, ... | 1997 | 9090691 |
| disc susceptibility testing for thermomphilic campylobacters. | the minimum inhibitory concentrations (mics) and zone diameters around nccls strength discs of 100 clinical isolates of thermophilic campylobacter species, including 79 strains of campylobacter jejuni subsp. jejuni, 19 of c. coli and two of c. lari, plus three type strains of these species, were determined for erythromycin, clindamycin, nalidixic acid, norfloxacin, ciprofloxacin, ampicillin, piperacillin, cephalothin, ceftriaxone, chloramphenicol, gentamicin and tetracycline. using error-rate bo ... | 1997 | 9213344 |
| fatal campylobacter jejuni infection in a patient splenectomised for thalassaemia. | a 35 year old man with a fatal campylobacter jejuni infection is described. he had hbe/beta zero thalassaemia and had undergone splenectomy nine months previously for hypersplenism; he also had chronic hepatitis c infection. he presented with high grade fever but no gastrointestinal symptoms and rapidly progressed to septicaemic shock and hepatic encephalopathy despite treatment with penicillin, gentamicin, and, later, chloramphenicol and ceftazidime. only one case of campylobacter jejuni septic ... | 1997 | 9215130 |
| stimulation of peripheral blood lymphocytes with campylobacter jejuni generates a gammadelta t cell response in patients with guillain-barrésyndrome. | in three patients whose guillain-barré syndrome (gbs) was preceded by gastrointestinal infection due to campylobacter jejuni, gammadelta t cells were generated from peripheral blood in response to in vitro stimulation with c. jejuni. in one of the patients, where a diagnostic sural nerve biopsy was performed, gammadelta t cells were also isolated following culture of the nerve tissue. studies with healthy volunteers and c. jejuni gastroenteritis patients also showed preferential enrichment for g ... | 1997 | 9218834 |
| identification and molecular cloning of a gene encoding a fibronectin-binding protein (cadf) from campylobacter jejuni. | campylobacter jejuni, a gram-negative bacterium, is a common cause of gastrointestinal disease. by analogy with other enteric pathogens such as salmonella and shigella, the ability of c. jejuni to bind to host cells is thought to be essential in the pathogenesis of enteritis. scanning electron microscopy of infected int407 cells suggested that c. jejuni bound to a component of the extracellular matrix. binding assays using immobilized extracellular matrix proteins and soluble fibronectin showed ... | 1997 | 9220003 |
| a new cytolethal distending toxin (cdt) from escherichia coli producing cnf2 blocks hela cell division in g2/m phase. | escherichia coli strain 1404, isolated from a septicaemic calf, carries a transferable plasmid called pvir which codes for the cytotoxic necrotizing factor type 2 (cnf2). a 4h interaction between strain 1404 and hela cells induced the formation of giant mononucleated cells blocked in g2/m phase. mating experiments between strain 1404 and a non-pathogenic recipient strain demonstrated that the factor(s) encoded by pvir mediated the cell-cycle arrest. a 3.3 kb dna fragment isolated from a dna bank ... | 1997 | 9220015 |
| comparison of disc diffusion and agar dilution methods for antibiotic susceptibility testing of campylobacter jejuni subsp. jejuni and campylobacter coli. | the correlation between disc diffusion and agar dilution susceptibility testing of five antibiotics was studied against 145 campylobacter strains: 99 campylobacter jejuni subsp. jejuni and 46 campylobacter coli. the percentages of true results and 95% ci for disc diffusion for resistant strains were 100% (93.2-100%) for tetracycline (53 strains tested), 100% (77.2-100%) for ciprofloxacin (13 strains tested), 86.7% (62.1-96.3%) for nalidixic acid (15 strains tested), 100% (56.6-100%) for erythrom ... | 1997 | 9222038 |
| association of campylobacter jejuni serotype with antiganglioside antibody in guillain-barré syndrome and fisher's syndrome. | using penner's method and lior's scheme, we serotyped 31 isolates from patients with guillain-barré syndrome, 7 isolates from those with fisher's syndrome, and isolates from patients with sporadic enteritis. pen 19 of campylobacter jejuni was isolated more frequently from the guillain-barré syndrome patients (16/31 isolates, 52%) than from the sporadic enteritis patients (5%). lio 7 of c. jejuni also was isolated more frequently from the gillain-barré syndrome patients (14/31 isolates, 45%) than ... | 1997 | 9225682 |
| toxin production by campylobacter spp. | of all the virulence factors that were proposed for campylobacter jejuni and related species to cause disease in humans, the discovery of toxin production was the most promising but led to a rather confusing and even disappointing stream of data. the discussion of whether proteinaceous exotoxins are relevant in disease remains open. one important reason for this lack of consensus is the anecdotal nature of the literature reports. to provide a basis for an unbiased opinion, this review compiles a ... | 1997 | 9227862 |
| molecular discrimination between campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter upsaliensis by polymerase chain reaction based on a novel putative gtpase gene. | polymerase chain reaction (pcr) mediated dna fingerprinting has resulted in the identification of a novel campylobacter jejuni gene, encoding a gtpase protein. the gene, consisting of 383 amino acids contained semi-conserved gtp-binding sites (designated g-1 to g-4), that are characteristic for members of the gtpase protein superfamily. remarkably, this gene from c. jejuni appears to encode a member of a novel family of gtp-binding proteins, containing two separate putative gtp-binding domains, ... | 1997 | 9232616 |
| differentiation of campylobacter jejuni and campylobacter coli by polymerase chain reaction. | a multiplex pcr assay was developed using two primer sets for the identification and differentiation of campylobacter coli and campylobacter jejuni. primer set i amplifies a 460-bp fragment present in c. coli and c. jejuni. set ii amplifies a 160-bp target unique to c. jejuni. when the assay was performed on reference strains, amplification of c. coli yielded only the 460-bp fragment. amplification of c. jejuni generated both the 160- and 460-bp fragments. campylobacter field strains (n = 85) is ... | 1997 | 9232618 |
| evaluation of the nasba nucleic acid amplification system for assessment of the viability of campylobacter jejuni. | although nasba uses rna as a target molecule for amplification, the nucleic acid amplification system cannot be used for differentiating viable and non-viable c. jejuni. it was shown that 16s rrna, or the defined sequence within the 16s rrna enclosed by the primer set applied, is fairly stable and resistant to heating at 100 degrees c. false-positive results can occur in case of direct nasba detection of c. jejuni in foods. these false positive results due to amplification of rna from dead cells ... | 1997 | 9237117 |
| characterization of monoclonal antibodies for the rapid detection of foodborne campylobacters. | the specificity of 97 monoclonal antibodies (mabs) to the campylobacter jejuni lior serogroup 6 reference strain was assessed using an indirect enzyme linked immunosorbent assay (elisa). four mabs, m316, m337, m357 and m637, reacted with whole cells of the c. jejuni, c. coli and c. lari reference strains of the 20 most common lior serogroups and 25 recent c. jejuni and c. coli isolates, and did not react with most of the 42 other campylobacter and non-campylobacter spp. tested. immunoblot analys ... | 1997 | 9237126 |
| [campylobacter jejuni septicemia with cutaneous sites in a patient with human immunodeficiency virus infection]. | 1997 | 9161581 | |
| primary structure analysis and adhesion studies on the major outer membrane protein of campylobacter jejuni. | the major outer membrane protein of campylobacter jejuni (momp, 43 kda), supposed to be one of the structures responsible for adhesion to int 407 cells, was isolated from the crude outer membrane preparation by treatment with n-octyl-beta-d-glucopyranoside followed by preparative sds-polyacrylamide gel electrophoresis. by cleavage of the isolated protein with cyanogen bromide and proteolytic enzymes, peptides were generated, separated by reverse phase high pressure liquid chromatography, and seq ... | 1997 | 9163918 |
| resuscitation of 'non-culturable' cells from aged cultures of campylobacter jejuni. | when stationary phase batch cultures of campylobacter jejuni were stored in sealed flasks under static conditions, viable numbers declined from 2 x 10(9) c.f.u. ml-1 to around 10(3)-10(6) c.f.u. ml-1 within 4-6 weeks. when the aged cultures were sparged with a microaerobic gas mixture, there was a rapid increase in viable numbers accompanied by a change from predominantly coccoid to vibrioid morphology. the most probable number (mpn) technique was used to distinguish resuscitation of injured or ... | 1997 | 9168608 |
| changes with growth rate in the membrane lipid composition of and amino acid utilization by continuous cultures of campylobacter jejuni. | methods and media (defined and complex) are described which permit studies designed to determine the influence of single environmental factors on the survival and virulence of campylobacter jejuni. the effect of growth rate on selected physiological traits (amino acid utilization, membrane lipid composition, motility, cell morphology) was studied in continuous culture. in both media, growth was at the expense of amino acid (serine, aspartate, glutamate and proline) catabolism. slow growth in the ... | 1997 | 9172406 |
| fulminant guillain-barré syndrome with universal inexcitability of peripheral nerves: a clinicopathological study. | the pathological basis of nerve inexcitability in guillain-barré syndrome has not been established with certainty. we report the clinicopathological findings in a 67-year-old patient with fulminant guillain-barré syndrome who died 18 days after onset. three serial electrophysiological studies revealed nerve inexcitability. antibodies to campylobacter jejuni were present but there was no antiganglioside reactivity. spinal root sections revealed extensive and almost pure macrophage-associated demy ... | 1997 | 9179157 |
| campylobacter jejuni gastroenteritis in turkish children. | four hundred children between the ages of 1 month and 14 years with the complaint of diarrhea were studied to assess campylobacter jejuni isolation rates in childhood acute gastroenteritis in turkey and to clarify clinical presentations of c. jejuni enteritis. c. jejuni was found to be the second most common isolate with a rate of 8.3%, the first being shigella strains. the highest isolation rate was in the 6 to 14-year age range at 12%. the most frequent symptoms in patients with c. jejuni ente ... | 1997 | 9181383 |
| oral administration of antibodies as prophylaxis and therapy in campylobacter jejuni-infected chickens. | passive immunity against gastrointestinal infections has recently been successfully applied as prophylaxis and therapy in patients in a variety of virally and bacterially induced infections. campylobacter jejuni is frequently associated with acute diarrhoea in humans, and several species of animals have been shown to transmit the disease, although birds have been implicated as the main source of infection. we used bovine and chicken immunoglobulin preparations from the milk and eggs, respectivel ... | 1997 | 9182891 |
| cloning, sequencing, and expression of a campylobacter jejuni periplasmic binding protein (p29) involved in histidine transport. | 1997 | 9192026 | |
| serendipitous detection of persistent campylobacter jejuni subspecies jejuni bacteremia in a patient undergoing bone marrow transplantation. | 1997 | 9195103 | |
| nested polymerase chain reaction strategy simultaneously targeting dna sequences of multiple bacterial species in inflammatory joint diseases. i. screening of synovial fluid samples of patients with spondyloarthropathies and other arthritides. | bacteria play a crucial pathogenetic role in lyme arthritis (la), reactive arthritis (rea), other forms of spondyloarthropathy (spa), and possibly in undifferentiated oligoarthritis (uoligo). polymerase chain reaction (pcr) technology has been applied to detect bacterial dna of individual microbes in synovial fluid (sf) of patients with arthritides. we screened for dna sequences of 8 bacterial species simultaneously in sf of patients with inflammatory joint disease. | 1997 | 9195515 |
| nested polymerase chain reaction strategy simultaneously targeting dna sequences of multiple bacterial species in inflammatory joint diseases. ii. examination of sacroiliac and knee joint biopsies of patients with spondyloarthropathies and other arthritides. | bacteria play a crucial pathogenetic role in reactive arthritis (rea) and other forms of spondyloarthropathy (spa) and in lyme arthritis. although there is evidence of local persistence of bacterial antigens no definitive method revealing microbes in peripheral joints has been established. we detected dna of individual bacteria in synovial material by pcr. applying molecular technology we screened simultaneously for 8 bacterial genomes in arthritis and sacroiliitis. | 1997 | 9195516 |
| campylobacter 0:41 isolation in guillain-barré syndrome. | over a period of 15 months, 17 children were admitted to the red cross war memorial children's hospital (rcwmch) in cape town with guillain-barré syndrome. stool specimens were collected from 14 children and campylobacter was isolated from nine. six of the nine isolates of campylobacter jejuni were further identified as c jejuni biotype 2, serotype 0:41. this biotype 2, serotype 0:41 has been identified in only 12 of the 7119 campylobacter isolates at the rcwmch over a 19 year period. eight of t ... | 1997 | 9245852 |
| overproduction of campylobacter ferritin in escherichia coli and induction of paracrystalline inclusion by ferrous compound. | the ferritin gene (cft) of campylobacter jejuni was overexpressed in cells of escherichia coli using a t7 rna polymerase expression system. many round particles which were the same size as the ferritin particles purified from c. jejuni were observed in the lysate of the cft-overexpressed e. coli cells. since most of them were devoid of a central electron dense core consisting of ferric irons, the campylobacter ferritins over-produced in e. coli seemed to be apoferritin. when large amounts of fer ... | 1997 | 9251057 |
| restriction endonuclease analysis, dna relatedness and phenotypic characterization of campylobacter jejuni and camp. coli isolates involved in food-borne disease. | seven cases of campylobacter infection, each of them involving two isolates, were analysed. study of their biochemical profiles and susceptibility patterns allowed the identification of camp. jejuni and camp. coli isolates and the effective typing of camp. jejuni strains into biotypes. genotyping was carried out by comparing chromosomal dna restriction patterns obtained by cleavage with bg/ii and ecorv and by southern hybridization experiments. these studies revealed clonal homogeneity between b ... | 1997 | 9202438 |
| helicobacter pylori induces an array of pro-inflammatory cytokines in human gastric epithelial cells: quantification of mrna for interleukin-8, -1 alpha/beta, granulocyte-macrophage colony-stimulating factor, monocyte chemoattractant protein-1 and tumour necrosis factor-alpha. | despite the fact that helicobacter pylori is known to be non-invasive, mucosal infiltration of inflammatory cells have been observed in the gastric mucosa. the exact pathogenesis of such an inflammatory reaction has not been well defined. we explored the repertoire of cytokine genes expressed in human gastric epithelial cells in response to coculture with h. pylori. after gastric epithelial cells, snu-5 and kato iii, were infected with h. pylori, expression of several cytokine genes was assessed ... | 1997 | 9257236 |
| identification of a common structural motif in the disordered n-terminal region of bacterial flagellins--evidence for a new class of fibril-forming peptides. | flagellin proteins lacking the n- or c-terminus form polymers of reduced filament stability and straight morphology, in contrast to the coiled native flagella. in the present study, the n-terminal amino acid sequence of flagellins of the anaerobic beer spoilage bacteria pectinatus cerevisiiphilus and pectinatus frisingiensis as well as enterobacter aerogenes and pseudomonas sp. were determined. sequence similarity was revealed between these and the n-termini of all known eubacterial flagellins. ... | 1997 | 9431986 |
| campylobacter jejuni major outer membrane protein and a 59-kda protein are involved in binding to fibronectin and int 407 cell membranes. | campylobacter jejuni is one of the major causes of human diarrhea throughout the world. attachment to host cells and extracellular matrix proteins is considered to be an essential primary event in the pathogenesis of enteritis. outer membrane proteins of three c. jejuni strains, one of which was aflagellate, were investigated for their contribution to the process of adhesion to int 407 cell membranes and the extracellular matrix protein fibronectin. using a ligand-binding immunoblotting assay th ... | 1997 | 9435102 |
| lethal maternal sepsis caused by campylobacter jejuni: pathogen preserved in placenta and identified by molecular methods. | campylobacter jejuni is one of the most common organisms in the etiology of febrile diarrhea. bacteremia is reported to be rare. in pregnant women, however, c. jejuni (previous name, vibrio fetus) can cause fetal death. we report the case of a pregnant woman with enterocolitis and sepsis that caused the death of the fetus and, 11 days later, the death of the mother. c. jejuni was detected by culture techniques at the time of the first symptoms but not during the subsequent course of disease. bac ... | 1997 | 9436972 |
| a structural analysis of the regularly arranged porin on the outer membrane of campylobacter jejuni based on correlation averaging. | a negatively stained electron micrograph of regularly arranged porin proteins of campylobacter jejuni on the isolated outer membrane of bacteria was analyzed in detail by the correlation averaging method using a computer-assisted program. the results showed that the porin of c. jejuni had a trimeric structure separated by about 10.4 +/- 0.15 nm. in addition, the pores in the trimers were also separated by about 4.3 +/- 0.1 nm. | 1997 | 9444326 |
| comparison of a novel microaerobic system with three other gas-generating systems for the recovery of campylobacter species from human faecal samples. | three commercial gas-generating systems--campygen (oxoid, uk), oxoid br56 (oxoid, uk), and campypak plus (becton dickinson, usa)--and the evacuation replacement technique were compared for the recovery of campylobacter spp. from 500 human faecal samples collected from patients with gastroenteritis. four hundred fifty faecal samples were tested upon receipt in the laboratory. fifty faecal samples that had been previously found to be positive for campylobacter spp. were tested retrospectively; the ... | 1997 | 9447907 |
| effect of haemin on the enterotoxin production of campylobacter jejuni. | when campylobacter jejuni was exposed to iron compounds, alterations in enterotoxin production were monitored by enzyme linked immunosorbent assay (elisa) and western blotting. by elisa, treatment with 10 microm haemin increased the ganglioside binding activity of the toxin in the extracellular fraction but not in the intracellular fraction. by western blotting, a 68 kd subunit of the enterotoxin was observed to increase in concentration. the results suggest that haemin may have a positive regul ... | 1997 | 9589608 |
| rapid identification of campylobacter jejuni strains by polymerase chain reaction & their restriction fragment length polymorphism analysis. | a polymerase chain reaction (pcr) technique was developed for specific identification of c. jejuni. a primer pair of a conserved region of flagellin a (fla a) gene identified all 15 strains of c. jejuni isolated from human faeces. none of the control strains like helicobacter pylori, vibrio cholerae escherichia coli and salmonella typhimurium except c. coli exhibited any amplified product by pcr. a predicted 450 bp could also be amplified from 4 chicken caecal contents positive for c. jejuni-c c ... | 1997 | 9029829 |
| identification of a functional homolog of the escherichia coli and salmonella typhimurium cysm gene encoding o-acetylserine sulfhydrylase b in campylobacter jejuni. | the final step of l-cysteine biosynthesis in escherichia coli and salmonella typhimurium consists of the formation of l-cysteine from o-acetylserine and sulfide. this reaction can be catalyzed by two enzymes, o-acetylserine sulfhydrylase a and o-acetylserine sulfhydrylase b, the former of which has been more rigorously characterized. in contrast to o-acetylserine sulfhydrylase a, o-acetylserine sulfhydrylase b is preferentially used for cysteine biosynthesis during anaerobic growth and is able t ... | 1997 | 9034314 |
| direct detection of campylobacter jejuni in chicken cecal contents by pcr. | campylobacter jejuni in chicken feces was detected by pcr and southern blot hybridization (sbh). the detection limits of c. jejuni in chicken feces were 34,000 cells by pcr and 340 cells by sbh. some cecal contents of chickens up to 3 weeks old were c. jejuni positive by sbh whereas all of them were negative by pcr. two of 51 cecal contents of 18-day-old chicken embryos were c. jejuni positive by pcr and sbh; but, c. jejuni were not isolated from the samples by conventional culture with selectiv ... | 1997 | 9035088 |
| multifocal motor neuropathy with conduction block after campylobacter jejuni enteritis. | 1997 | 9040761 | |
| cross-reactive antibodies against gangliosides and campylobacter jejuni lipopolysaccharides in patients with guillain-barré or miller fisher syndrome. | campylobacter jejuni was isolated from stool specimens of 3 patients with miller fisher syndrome (mfs) and 2 patients with guillain-barré syndrome (gbs). anti-gq1b antibodies in serum from all mfs patients cross-reacted with sialidase-sensitive epitopes in the lipopolysaccharide fraction of c. jejuni from these 3 mfs patients. one gbs patient had anti-gm1 antibodies that bound with lipopolysaccharide of c. jejuni from a control patient and from the other gbs patient without anti-gm1 antibodies. ... | 1997 | 9041356 |
| specific identification of the enteropathogens campylobacter jejuni and campylobacter coli by using a pcr test based on the ceue gene encoding a putative virulence determinant. | a pcr method for the rapid identification and discrimination of thermophilic campylobacter jejuni and campylobacter coli was developed by using a gene encoding a protein involved in siderophore transport (ceue). a nucleotide sequence divergence of approximately 13% in the ceue genes of c. jejuni and c. coli facilitated the design of two species-specific pcr primer sets. the specificity of the pcr amplification reactions was confirmed by using two nonradioactively labelled species-specific intern ... | 1997 | 9041429 |
| n-nitrosation of medicinal drugs catalysed by bacteria from human saliva and gastro-intestinal tract, including helicobacter pylori. | micro-organisms commonly present in human saliva and three dsm strains (helicobacter pylori, campylobacter jejuni and neisseria cinerea), which can be isolated from the human gastro-intestinal tract, were assayed in vitro for their capacity to catalyse n-nitrosation of a series of medicinal drugs and other compounds. following incubation at ph 7.2 in the presence of nitrate (or nitrite) for up to 24 (48) h, the yield of n-nitroso compounds (noc) was quantified by hplc equipped with a post-column ... | 1997 | 9054633 |
| use of an arbitrarily primed pcr product in the development of a campylobacter jejuni-specific pcr. | development of a pcr assay for campylobacter jejuni is based on the isolation of species-specific dna. an arbitrarily primed pcr incorporating 10-mer primers was used to generate fingerprints of c. jejuni m129 genomic dna. fingerprint products were then screened individually for their species specificity in dot blot hybridizations with 6 c. jejuni isolates, 4 campylobacter species other than c. jejuni, and 27 enteric bacterial species other than campylobacter spp. a 486-bp fingerprint product hy ... | 1997 | 9055418 |
| cloning and nucleotide sequence of the gyra gene from campylobacter fetus subsp. fetus atcc 27374 and characterization of ciprofloxacin-resistant laboratory and clinical isolates. | the gyra gene of campylobacter fetus subsp. fetus, which encodes the a subunit of dna gyrase, was cloned, and its nucleotide sequence was determined. an open reading frame of 2,586 nucleotides which encodes a polypeptide of 862 amino acids with an mr of 96,782 was identified. c. fetus subsp. fetus gyra is most closely related to campylobacter jejuni gyra, with 73% homology at the nucleotide level and 78% identity between polypeptides. the next most closely related gyra was that from helicobacter ... | 1997 | 9056011 |
| campylobacter jejuni seasonal recovery observations of retail market broilers. | this study investigated possible seasonal trends in the campylobacter jejuni carrier state of market broilers. in this study, broiler carcasses, 15 each of two major companies, were obtained from a local supermarket each month for an entire year to evaluate the presence of c. jejuni on the carcasses. direct plating and the whole carcass rinse procedure were used for c. jejuni detection. resuscitation of damaged cells and preenrichment of low numbers of micoorganisms were accomplished by hunt's p ... | 1997 | 9057212 |
| reactivity of serum igg anti-gm1 ganglioside antibodies with the lipopolysaccharide fractions of campylobacter jejuni isolates from patients with guillain-barre syndrome (gbs). | campylobacter jejuni (cj) enteritis is the most frequently recognised infection preceding guillain-barre syndrome (gbs) and this combination is commonly associated with anti-gm1 ganglioside (anti-gm1) antibodies. we have examined the hypothesis that the anti-gm1 antibodies represent an immune response against the cj lipopolysaccharide (lps). we prepared the lps fraction from 8 isolates of cj, 3 from gbs patients with acute inflammatory demyelinating polyradiculoneuropathy (aidp), 3 from patients ... | 1997 | 9058756 |
| immunological investigation of chronic inflammatory demyelinating polyradiculoneuropathy. | in order to investigate the hypothesis that chronic inflammatory demyelinating polyradiculoneuropathy (cidp) is an autoimmune disease related to the acute inflammatory form of guillain-barre syndrome (gbs), we studied 40 patients, 40 age and sex matched controls with other forms of peripheral neuropathy (onp) and 37 controls from the same family or household (fc). we sought antibodies to gangliosides gm1 and lm1 by enzyme linked immunoassay (elisa) confirmed by immuno-overlay. only 6 (15%) cidp ... | 1997 | 9058768 |
| lineages within campylobacter jejuni defined by numerical analysis of pulsed-field gel electrophoretic dna profiles. | forty-seven penner heat-stable (hs) serotype reference strains for campylobacter jejuni and 47 serologically non-typable strains were examined by pulsed field gel electrophoresis (pfge) dna restriction analysis. the smai and kpni digest profiles were compared by numerical analysis. most strains grouped differently in the two analyses but strain lineages were inferred where the two agreed. genetic relationships between reference strains in the cross-reacting hs4 complex were examined. three clona ... | 1997 | 9060876 |
| motor nerve terminal degeneration provides a potential mechanism for rapid recovery in acute motor axonal neuropathy after campylobacter infection. | we investigated the possible mechanisms of paralysis and recovery in a patient with the acute motor axonal neuropathy (aman) pattern of the guillain-barré syndrome. the aman pattern of gbs is characterized clinically by acute paralysis without sensory involvement and electrodiagnostically by low compound motor action potential amplitudes, suggesting axonal damage, without evidence of demyelination. many aman patients have serologic or culture evidence of recent campylobacter jejuni infection. pa ... | 1997 | 9065554 |
| hydrophobic characterization of thermophilic campylobacter species and adhesion to int 407 cell membranes and fibronectin. | cell surface hydrophobicity of campylobacter jejuni, c. coli, c. lari and c. upsaliensis was tested by hydrophobic interaction chromatography on octylsepharose cl-4b. the hydrophobicity was influenced by cultivation mode, presence or absence of intact lipopolysaccharide (lps) and outer membrane protein structures. species-specific differences of hydrophobic characteristics were not detected. bacteria grown in fluid medium exhibited a high degree of hydrophobicity. agar-grown bacteria showed hydr ... | 1997 | 9075218 |
| chey-mediated modulation of campylobacter jejuni virulence. | four motile, non-adherent and non-invasive mutants of campylobacter jejuni 81-176 generated by a site-specific insertional mutagenesis scheme were characterized at the molecular level and all contained a duplication of the same region of the chromosome. when this region was cloned from wild-type 81-176 and transferred into 81-176 on a shuttle plasmid, the same non-invasive phenotype as the original mutants was observed, suggesting that the region contained a repressor of adherence and invasion. ... | 1997 | 9076738 |
| genotypic diversity among campylobacter jejuni isolates in a commercial broiler flock. | analysis of nucleic acid polymorphism in the flagellin genes of campylobacter jejuni was used to investigate genetic diversity among campylobacter spp. in a commercial broiler flock. three hundred single colonies of c. jejuni were isolated from fecal samples collected weekly for 3 weeks immediately before slaughter. both the flaa and flab genes were amplified by pcr, and the pcr product was digested with the restriction enzyme alui. the fragments generated were then analyzed by agarose gel elect ... | 1997 | 16535602 |
| [in process citation]. | oral delivery of vaccines results in these being taken up by specialised microfold epithelial cells covering peyer's patches of the gastrointestinal tract, therefore stimulating regulatory t cells and surface iga positive (siga+) b cells. t helper cells can be divided into 2 subsets, type 1 (t(h)1) and type 2 (t(h)2), according to their function and the cytokines they secrete. t(h)1 cytokines such as interleukin (il)-2, interferon-gamma and tumour necrosis factor-beta (tnfbeta) elicit activation ... | 1997 | 18031087 |
| preferential interaction of the his pause rna hairpin with rna polymerase beta subunit residues 904-950 correlates with strong transcriptional pausing. | rna secondary structures (hairpins) that form as the nascent rna emerges from rna polymerase are important components of many signals that regulate transcription, including some pause sites, all rho-independent terminators, and some antiterminators. at the his leader pause site, a 5-bp-stem, 8-nt-loop pause rna hairpin forms 11 nt from the rna 3' end and stabilizes a transcription complex conformation slow to react with ntp substrate. this stabilization appears to depend at least in part on an i ... | 1997 | 9237994 |
| carbon load in aquatic ecosystems affects the diversity and biomass of water biofilm consortia and the persistence of the pathogen campylobacter jejuni within them. | the influence of carbon load on autochthonous water microflora population distribution and diversity, and on the persistence of campylobacter jejuni, was examined with a two-stage aquatic biofilm model. serine was chosen since it is a carbon source utilised by c. jejuni and concentrations were chosen to reflect upper limits of amino acid load reported in surface water. the total viable count of the autochthonous biofilm microflora increased with increasing serine concentration (10-fold and 20-fo ... | 1998 | 21182705 |
| serological evidence for infection with campylobacter jejuni/coli in patients with multifocal motor neuropathy. | campylobacter jejuni/coli (cjc) infection has been implicated in the immunopathogenesis of guillain-barré syndrome (gbs), acute motor axonal neuropathy (aman), and miller fisher syndrome (mfs). however, its role in chronic immune mediated neuropathies such as multifocal motor neuropathy (mmn) or chronic inflammatory demyelinating polyradiculoneuropathy (cidp) is less clear. anti-ganglioside antibodies are associated with chronic motor neuropathies such as mmn and igm anti-gm1, and igm anti-asial ... | 1998 | 18644284 |
| incidental finding of myxobolus spores (protozoa: myxozoa) in stool samples from patients with gastrointestinal symptoms. | myxozoan spores were detected in fecal samples from three patients presenting with abdominal pain and/or diarrhea. the spores were identical to those of myxobolus plectroplites, a previously described pathogen from the freshwater fish plectroplites ambiguus. all patients had recently eaten fish caught from local waters, and frozen fillets of such fish were found to be infected with m. plectroplites cysts. the passage of spores unchanged through the alimentary tract suggests they were incidental ... | 1998 | 9817910 |
| inflammatory bowel disease: definition, epidemiology, etiologic aspects, and immunogenetic studies. | 1998 | 11819343 | |
| communication behaviours in a hospital setting: an observational study. | an exploratory study to identify patterns of communication behaviour among hospital based healthcare workers. | 1998 | 9522794 |
| characterization of campylobacter spp. using restriction fragment length polymorphism and sds-polyacrylamide gel electrophoresis. | twenty four campylobacter jejuni and coli isolates obtained from egyptian children were characterized using restriction fragment length polymorphism (rflp) of flagellin genes and polyacrylamide gel electrophoresis of whole cell and glycine-extracted proteins. the isolates were found to fall into nine polymorphism groups, eight of which were reported previously in egypt but one group displayed by 3 isolates represented a new group that was not reported before. furthermore, the relative prevalence ... | 1998 | 17249207 |
| effect of temperature on adhesion of vibrio strain ak-1 to oculina patagonica and on coral bleaching. | laboratory aquarium experiments demonstrated that vibrio strain ak-1 caused rapid and extensive bleaching of the coral oculina patagonica at 29 degrees c, slower and less-complete bleaching at 23 degrees c, and no bleaching at 16 degrees c. at 29 degrees c, the application of approximately 100 vibrio strain ak-1 cells directly onto the coral caused 50 and 83% bleaching after 10 and 20 days, respectively. at 16 degrees c, there was no bleaching, even with an initial inoculum of 1.2 x 10 bacteria. ... | 1998 | 16349542 |
| water-borne campylobacter jejuni infection in a danish town---a 6-week continuous source outbreak. | objective: to determine the cause and characteristics of illness of a campylobacter jejuni outbreak in denmark in 1995--96. methods: a retrospective follow-up study was designed for culture-confirmed cases and for residents without a bacteriologic diagnosis. stored clinical and environmental isolates were analyzed by serotyping and genotyping with restriction endonuclease analysis (rea), pulsed-field gel electrophoresis (pfge) and ribotyping. results: campylobacter jejuni was isolated from 110 r ... | 1998 | 11864264 |
| anti-ganglioside antibody and neuropathy: review of our research. | some patients developed guillain-barré syndrome (gbs) after the administration of bovine gangliosides. patients with gbs subsequent to campylobacter jejuni enteritis frequently have igg antibody to gm1 ganglioside. fisher's syndrome (fs), a variant of gbs, is associated with igg antibody to gq1b ganglioside. we showed the existence of molecular mimicry between gm1 and lipopolysaccharide (lps) of c. jejuni isolated from a gbs patient, and that between gq1b and c. jejuni lpss from fs patients. sev ... | 1998 | 10959233 |
| iron storage in bacteria. | iron is an essential nutrient for nearly all organisms but presents problems of toxicity, poor solubility and low availability. these problems are alleviated through the use of iron-storage proteins. bacteria possess two types of iron-storage protein, the haem-containing bacterioferritins and the haem-free ferritins. these proteins are widespread in bacteria, with at least 39 examples known so far in eubacteria and archaebacteria. the bacterioferritins and ferritins are distantly related but ret ... | 1998 | 9889981 |
| campylobacter jejuni infection and guillain-barré syndrome: a case-control study. emilia-romagna study group on clinical and epidemiological problems in neurology. | we performed a case-control study to investigate the association between campylobacter jejuni (cj) infection and guillain-barr| syndrome (gbs) or miller-fisher syndrome. we compared 60 cases with 109 hospital controls matched for age, gender, hospital and geographical location. to diagnose the cj infection, we considered the association between serologic positivity for cj and a previous diarrheal illness within 3 months of inclusion in the study. fifteen percent of cases versus 5% of hospital co ... | 1998 | 9890808 |
| campylobacter jejuni bacteremia and guillain-barré syndrome in a renal transplant recipient. | in patients who have not undergone transplantation, guillain-barré syndrome (gbs) is typically preceded by an acute infection often sustained by campylobacter jejuni. thus far, in renal transplant recipients, only eight cases of gbs have been reported. in seven patients gbs was attributed to cytomegalovirus infection and in the eighth patient to cyclosporin a neurotoxicity. we report here the case of a gbs in a renal transplant recipient following c. jejuni bacteremia. the infection quickly disa ... | 1998 | 9870273 |
| aerobic growth and survival of campylobacter jejuni in food and stream water. | when 40 campylobacter jejuni isolates from human clinical cases, raw chicken and water were tested, 29 (72.5%) could be adapted to grow on nutrient agar under aerobic conditions. once adapted, these isolates could grow on repeated aerobic subculture. an aerobically-grown camp. jejuni isolate survived almost as well as the same isolate grown microaerophilically in sterile chicken mince at 5 degrees c, and survival of a cocktail of camp. jejuni isolates under both atmospheres was comparable at 25 ... | 1998 | 9871351 |