Publications

green fluorescent protein as a probe of rotational mobility within bacteriophage t4.	green fluorescent protein (gfp) was targeted into bacteriophage t4 heads and proheads as a probe of the internal environment. targeting was accomplished with internal protein iii (ipiii) fusion proteins or capsid targeting sequence (cts)-tagged proteins, where cts is the 10-amino acid residue cts of ipiii. recombinant phage t4[cts/ipiii/gfp], t4[cts/ipiii(t)gfp], and t4[cts/gfp] packaged gfp fusion proteins and processed them at cleavage sites designated /. steady-state and time-resolved fluores ...	2000	10921840
sympathetic innervation alters activation of pacemaker current (if) in rat ventricle.	pacemaker current (if) exists in both neonatal and adult ventricles, but activates at more negative voltages in the adult. this study uses whole-cell patch clamp to investigate the factors that may contribute to the maturational shift of if, comparing neonatal rat ventricular myocytes that were cultured for 4-6 days either alone, in co-culture with sympathetic nerves, or with neurotransmitters chronically present in culture. if recorded from nerve-muscle co-cultures had a significantly more nega ...	2000	10922008
establishment of introduced antagonistic bacteria in the rhizosphere of transgenic potatoes and their effect on the bacterial community.	in a field release experiment, rifampicin resistant mutants of two antagonistic plant-associated bacteria were used for seed tuber inoculation of transgenic t4 lysozyme expressing potatoes, transgenic control potatoes and non-transgenic parental potatoes. the t4 lysozyme tolerant pseudomonas putida qc14-3-8 was originally isolated from the tuber surface (geocaulosphere) of t4 lysozyme producing plants and showed in vitro antibacterial activity to the bacterial pathogen erwinia carotovora ssp. at ...	2000	10922502
double-strand break repair in tandem repeats during bacteriophage t4 infection.	recombinational repair of double-strand breaks in tandemly repeated sequences often results in the loss of one or more copies of the repeat. the single-strand annealing (ssa) model for repair has been proposed to account for this nonconservative recombination. in this study we present a plasmid-based physical assay that measures ssa during bacteriophage t4 infection and apply this assay to the genetic analysis of break repair. ssa occurs readily in broken plasmid dna and is independent of the st ...	2000	10924452
dinucleotide repeat expansion catalyzed by bacteriophage t4 dna polymerase in vitro.	dna replication normally occurs with high fidelity, but certain "slippery" regions of dna with tracts of mono-, di-, and trinucleotide repeats are frequently mutation hot spots. we have developed an in vitro assay to study the mechanism of dinucleotide repeat expansion. the primer-template resembles a base excision repair substrate with a single nucleotide gap centered opposite a tract of nine ca repeats; nonrepeat sequences flank the dinucleotide repeats. dna polymerases are expected to repair ...	2000	10924513
cortical representation of pain: functional characterization of nociceptive areas near the lateral sulcus.	many lines of evidence implicate the somatosensory areas near the lateral sulcus (sylvian fissure) in the cortical representation of pain. anatomical tracing studies in the monkey show nociceptive projection pathways to the vicinity of the secondary somatosensory cortex in the parietal operculum, and to anterior parts of insular cortex deep inside the sylvian fissure. clinical observations demonstrate alterations in pain sensation following lesions in these two areas in human parasylvian cortex. ...	2000	10924804
does the combination of radiotherapy and debulking surgery favor survival in paranasal sinus carcinoma?	to determine the contribution of debulking surgery on local control and survival in paranasal sinus tumors. as most patients present with locally advanced disease, the possibility of radical surgery is limited. consequently, radiotherapy is often needed as monotherapy or as an adjunct to surgery.	2000	10924968
subclinical thyroid disease after radiation therapy detected by radionuclide scanning.	the actuarial risk for developing benign or malignant thyroid disease following radiation therapy (rt) is controversial, but may be as high as 50% at 20 years. an effective screening modality should be specific but not overly sensitive, a limitation of ultrasound. we questioned whether technetium-99 m pertechnetate ((99m)tc tco(4)(-)) scanning could detect clinically significant disease in ostensibly disease-free cancer survivors.	2000	10924988
importance of gluten in the induction of endocrine autoantibodies and organ dysfunction in adolescent celiac patients.	it is well known that a high number of celiac patients may develop autoantibodies against endocrine glands, but it has not yet been clarified if this increased autoimmune response and the impaired organ function that can develop may be related to the presence or absence of gluten in the diet. the aim of the present study was to evaluate the effect of gluten on the autoimmunity and function of the endocrine glands in adolescent celiac patients.	2000	10925978
effects of pneumoperitoneum and reverse trendelenburg position on cardiopulmonary function in morbidly obese patients receiving laparoscopic gastric banding.	we prospectively evaluated the effects of pneumoperitoneum and reverse trendelenburg position on cardiopulmonary function in 20 asa physical status ii-iii morbidly obese patients (body mass index > 35 kg m(-2)) undergoing laparoscopic gastric banding. after general anaesthesia was induced, patients' lungs were ventilated using intermittent positive pressure ventilation (at measurement times, the following parameters were used: tidal volume 12 ml kg(-1) ideal body weight, respiratory rate of 12 b ...	2000	10926070
function of tyrosyl-trna synthetase in splicing group i introns: an induced-fit model for binding to the p4-p6 domain based on analysis of mutations at the junction of the p4-p6 stacked helices.	we used an escherichia coli genetic assay based on the phage t4 td intron to test the ability of the neurospora crassa mitochondrial tyrosyl-trna synthetase (cyt-18 protein) to suppress mutations that cause structural defects around its binding site in the p4-p6 domain of the group i intron catalytic core. we analyzed all possible combinations of nucleotides at either p4 bp-1 or p6 bp-1, which together form the junction of the p4-p6 stacked helices, and looked for synergistic effects in double m ...	2000	10926509
hormonal regulation of glutathione s-transferase expression in co-cultured adult rat hepatocytes.	glutathione s-transferases (gsts) are subject to regulation by thyroid and sex hormones and by gh. we have used an in vitro experimental system comprising adult rat hepatocytes co-cultured with rat liver epithelial cells of primitive biliary origin, to distinguish between direct and indirect effects of various hormones on gsts; to identify the gst subunits affected by individual hormones; and to investigate the level at which the hormones act. tri-iodothyronine (t3), thyroxine (t4) and 17beta-oe ...	2000	10927625
atrial resection in advanced lung carcinoma under total cardiopulmonary bypass.	there are few reports of a surgical approach to t4 lung carcinoma that has invaded the heart. although most cases will be considered inoperable, cases in which there is potential for complete resection and no distant or nodal metastatic disease (t4 n0 m0, stage iiib) may be considered for surgical therapy. we report a case of squamous cell carcinoma of the lung with cardiac involvement, in which we performed a completion pneumonectomy using total cardiopulmonary bypass. we describe indications a ...	2000	10928496
thyroxine modulates corticotropin-releasing factor but not arginine vasopressin gene expression in the hypothalamic paraventricular nucleus of the developing rat.	neonatal rats were daily injected with 100 microg/kg t4 and killed at 4, 8 or 15 days. circulating corticosterone and corticosteroid binding globulin concentrations increased in 8- and 15-day-old rats after t4 treatment. plasma adrenocorticotropic hormone (acth) concentrations, pituitary acth content and pro-opiomelanocortin mrna expression were unaffected in t4-treated rats. t4 treatment induced an increase in corticotropin-releasing factor (crf) mrna expression in the whole population of crf s ...	2000	10929090
reduced muscle vascular resistance in intrauterine growth restricted newborn piglets.	it has been shown that asymmetrical intrauterine growth restriction is denoted by disproportional reduction of muscle mass compared to body weight reduction. however, the effects of iugr on regional vascular resistance and blood flow of skeletal muscles and their contractile function have not been studied until now. therefore, muscle blood flow (mbf) and isometric force output of serial stimulated hindlimb plantar flexors was measured in thiopental -anesthetized normal weight (nw; n = 9) and int ...	2000	10930129
hemodynamics, intramucosal ph and regulators of circulation during perioperative epidural analgesia.	to evaluate the effects of perioperative epidural analgesia on hemodynamics, splanchnic perfusion and regulators of circulation.	2000	10930202
management of the nontoxic multinodular goitre: a european questionnaire study.	the optimum management strategy for the patient with a multinodular nontoxic goitre is still a matter of debate. our aim was to assess the attitudes towards management of such patients throughout europe by means of a questionnaire.	2000	10931075
thyroid function in wholly breast-feeding infants whose mothers take high doses of propylthiouracil.	propylthiouracil (ptu) might theoretically be preferred over methimazole (mmi) during breast-feeding because of its lower milk/serum concentration ratio (0.1 vs. 1.0). the problem is that graves' disease often relapses during the postpartum period, and high doses of ptu are sometimes needed to control maternal hyperthyroidism) during breast-feeding. however, there are virtually no data on the effects of maternal ptu on thyroid status of infants whose mothers take more than 300 mg ptu daily and w ...	2000	10931098
changes in serum thyroid hormones levels and their mechanisms during long-term growth hormone (gh) replacement therapy in gh deficient children.	the effects of gh therapy on thyroid function among previous reports have shown remarkable discrepancies, probably due to differences in hormone assay methods, degree of purification of former pituitary-derived gh preparations, dosage schedules, diagnostic criteria, patient selection, duration of treatment and study design. these considerations motivated us to investigate whether and how gh replacement therapy changes serum thyroid hormone levels, including the much less studied rt3 levels, in a ...	2000	10931099
[combined intercostal nerve block and epidural anesthesia in a patient with severe aortitis syndrome].	a 74-year-old woman with aortitis syndrome was scheduled for mastectomy. her left vertebral artery was totally occluded and left carotid arteries, left subclavia artery and bilateral common renal arteries were occluded. for anesthesia a catheter was inserted into the epidural space between t3 and t4. lidocaine 80 mg (2%, 4 ml) was administered epidurally. then intercostal nerve block was performed with bupivacaine 15 mg (0.5%, 3 ml) at t3 and t4 level. local infiltration of lidocaine 50 mg (1%, ...	2000	10933035
coexistent insulin dependent diabetes mellitus and hyperthyroidism in a patient with down's syndrome.	the prevalence of thyroid disease is increased in down's syndrome. compared with adults, thyroid dysfunction in children with down's syndrome is less frequently reported. insulin dependent diabetes mellitus is also uncommon in down's syndrome children. coexistent insulin dependent diabetes mellitus and hyperthyroidism in down's syndrome was only reported once previously in literature. we report an 8-year-old girl with down's syndrome that had polyuria, polydipsia, abdominal pain and urinary inco ...	2000	10933753
consequences of vertebral deformities in older men and women.	the objectives of this study were to ascertain the prevalence of the number and severity of vertebral deformities in elderly people and determine the extent to which these are associated with several aspects of functioning. the study was conducted in a subsample of the longitudinal aging study amsterdam (lasa) consisting of 527 participants (260 men and 267 women), aged 65 years or over. lateral radiographs of the spine (t4-l5) were made of each participant and a semiquantitative method was used ...	2000	10934655
[use of percutaneous cardiopulmonary support (pcps) for extended surgery in patients with t4 tumor].	since 1991, we have performed operations for tumors invading the upper airway, left atrium or main pulmonary artery with percutaneous cardiopulmonary support (pcps) stand by support available. of 15 cases with pcps stand by, 6 patients actually underwent operation using pcps. there were three esophageal cancers invading the carina, two lung neoplasms with left atrial invasion and one neoplasm extending to the main pulmonary artery. one of three patients with esophageal cancer had massive bleedin ...	2000	10935395
novel hepatoselective insulin analog: studies with a covalently linked thyroxyl-insulin complex in humans.	to test whether a thyroxyl-insulin analog with restricted access to receptor sites in peripheral tissues displays relative hepatoselectivity in humans.	2000	10937509
plant enzymes but not agrobacterium vird2 mediate t-dna ligation in vitro.	agrobacterium tumefaciens, a gram-negative soil bacterium, transfers dna to many plant species. in the plant cell, the transferred dna (t-dna) is integrated into the genome. an in vitro ligation-integration assay has been designed to investigate the mechanism of t-dna ligation and the factors involved in this process. the vird2 protein, which is produced in agrobacterium and is covalently attached to t-dna, did not, under our assay conditions, ligate t-dna to a model target sequence in vitro. we ...	2000	10938108
achyranthes aspera elevates thyroid hormone levels and decreases hepatic lipid peroxidation in male rats.	a study was made to evaluate the role of achyranthes aspera on the changes in serum thyroid hormone concentrations and glucose levels in male rats. an attempt was also made to establish the relationship between hepatic lipid peroxidation and extract induced changes in thyroid hormone concentration, if any. adult male wistar rats were orally administered with the aqueous leaf extract of achyranthes aspera at a dose of 200 mg/kg b. wt./day for 7 days. the effects of the extract on body weight, hep ...	2000	10940593
high-dose intra-arterial cisplatin and concurrent hyperfractionated radiation therapy in patients with locally advanced primary squamous cell carcinoma of the head and neck: report of a phase ii study.	this phase ii study evaluates the tolerability and efficacy of concurrent hyperfractionated radiation therapy (hfx-rt) and high-dose intra-arterial (ia) cisplatin in patients with locally advanced squamous cell carcinoma of the head and neck (scchn).	2000	10941154
[parasigmoid abscess revealed by anaerobic osteomyelitis].	we report the case of a 66 year-old-patient, who profited by a laminectomy t4 to t7 for spinal cord compression due to an osteomyelitis. bacteriological samples showed positives gram cocci and bacteroides fragilis by the culture. the systematic research of a primitive source permitted to find a parasigmoidis abscess, which was treated by a sigmoidectomy with a colorectal anastomosis.	2000	10941448
survival of patients with resected n2 non-small-cell lung cancer: evidence for a subclassification and implications.	patients who suffer from non-small-cell lung cancer (nsclc) with ipsilateral mediastinal lymph node involvement (n2) belong to a heterogeneous subgroup of patients. we analyzed the prognosis of patients with resected n2 nsclc to propose homogeneous patient subgroups.	2000	10944131
measured and calculated effects of mutations in bacteriophage t4 lysozyme on interactions in solution.	understanding the molecular determinants of protein interactions in solution has fundamental implications for understanding protein solution thermodynamics and, hence, processes as diverse as separations performance and cellular self-organization. our earlier theoretical calculations indicate that the protein-protein interactions are dominated by a small number of configurations in which highly complementary surface regions are apposed, rather than by the overall colloidal interactions. to exami ...	2000	10944399
[concurrent chemoradiotherapy for t4 and/or m1 lym squamous cell carcinoma of the esophagus].	a phase ii study was conducted to investigate the efficacy and feasibility of chemoradiotherapy for locally advanced carcinoma of the esophagus. fifty-four patients with clinical t4 and/or m1 lym squamous cell carcinoma of the esophagus were enrolled. patients received protracted infusions of fluorouracil 400 mg/m2/24 hours on days 1 to 5 and 8 to 12, 2-hour infusions of cisplatin 40 mg/m2 on days 1 and 8, and concurrent radiotherapy at a dose of 30 gy in 15 fractions over 3 weeks. filgrastim wa ...	2000	10945013
three-dimensional structural views of damaged-dna recognition: t4 endonuclease v, e. coli vsr protein, and human nucleotide excision repair factor xpa.	genetic information is frequently disturbed by introduction of modified or mismatch bases into duplex dna, and hence all organisms contain dna repair systems to restore normal genetic information by removing such damaged bases or nucleotides and replacing them by correct ones. the understanding of this repair mechanism is a central subject in cell biology. this review focuses on the three-dimensional structural views of damaged dna recognition by three proteins. the first protein is t4 endonucle ...	2000	10946233
the hypothalamic-pituitary-thyroid negative feedback control axis in children with treated congenital hypothyroidism.	measurements of serum concentrations of free t4, t3, tsh, and thyroglobulin (tg) were conducted in 42 infants (2-9 months of age) detected and treated through the northwest newborn regional screening program and 63 children and adolescents (1-18 yr of age) with congenital hypothyroidism (ch) detected and managed in the northern california kaiser permanente medical care program. normal feedback control axis data were developed by quest diagnostics, inc. - nichols institute diagnostics and loma li ...	2000	10946871
an elevation of serum immunoglobulin e provides a new aspect of hyperthyroid graves' disease.	in hyperthyroid graves' disease, short-term methimazole is sufficient to induce lasting remission in some patients, but even long-term treatment fails to do so in others. we have evaluated the role of autoimmune abnormalities in the helper t cell type 2 (th2)-interleukin-13 (il-13)-tsh receptor system in maintaining hyperthyroidism by comparing ige levels in patients with various thyroid diseases. one hundred and ninety-three patients with hyperthyroid graves' disease were treated with methimazo ...	2000	10946880
familial dysalbuminemic hyperthyroxinemia in a swiss family caused by a mutant albumin (r218p) shows an apparent discrepancy between serum concentration and affinity for thyroxine.	familial dysalbuminemic hyperthyroxinemia (fdh), is the most common cause of inherited increase in serum total t4 (tt4) in the caucasian population. it is caused by a mutation (r218h) in the human serum albumin (hsa) gene, resulting in 10-fold higher affinity for t4 and, in heterozygous affected subjects, a tt4 level 2-fold higher than that in subjects expressing the wild-type hsa only. we now report fdh in a swiss family, caused by hsa r218p, previously reported in subjects of japanese origin. ...	2000	10946882
characterization of the uridine diphosphate-glucuronosyltransferase-catalyzing thyroid hormone glucuronidation in man.	increased thyroid hormone glucuronidation in rats caused by exposure to xenobiotics has stimulated a search for the individual uridine diphosphate-glucuronosyltransferases (ugts) catalyzing this reaction in rats and man. microsomal preparations from crigler-najjar liver, normal human liver, and kidney have been used to try to identify the ugt isoforms responsible for glucuronidation of the thyroid hormones. the predominant thyroid hormone released from the thyroid gland, t4, and the inactive rt3 ...	2000	10946897
reevaluation of nonphytate phosphorus requirement of growing pullets with and without phytase.	an experiment was conducted to re-evaluate the nonphytate phosphorus (npp) requirement of growing pullets and to determine to what extent this requirement could be reduced by phytase. six diets in a 3 x 2 factorial design were used. the pullets of the control group (t1) were fed a sequence of 0.4-0.35-0.3% npp for the age periods of 0 to 6, 6 to 12, and 12 to 18 wk, respectively, according to nrc (1994) recommendation. the npp level was reduced by an increment of 0.1% in each phase in t2 and t3. ...	2000	10947183
transcriptional repression of trh promoter function by t3: analysis by in vivo gene transfer.	we consider how an integrated in vivo model can be used to study the specific transcriptional effects of specific receptors in neuroendocrine systems. our example is the role of thyroid receptor (tr) isoforms in mediating negative feedback effects of t3 on trh (thyrotropin releasing hormone) expression. the in vivo transfection method employed polyethylenimine (pei) to introduce genes directly into specific regions of the brains of mice, rats, and xenopus tadpoles. in the mouse model, the techni ...	2000	10949071
vitamin e supplementation attenuates leakage of enzymes following 6 successive days of running training.	the purpose of this study was to examine whether vitamin e supplementation in humans would attenuate an increase of serum enzymes as an indirect marker of muscle damage following a sudden large increase in the running distance in a 6-day running training or not. a randomized and placebo-controlled study was carried out on fourteen male runners who were supplied vitamin e (alpha-tocopherol 1200 iu x day(-1); e) or placebo (p) 4 weeks prior to (t1) and during 6 successive days of running training ...	2000	10950448
interaction between serum leptin levels and hypothalamo-hypophyseal-thyroid axis in patients with anorexia nervosa.	the main objective of the study was to evaluate the endocrinological picture of anorexia. serum leptin levels are low in untreated anorexia nervosa (an), but studies of the exact relationship between leptin, body weight and hormones of hypothalamo-hypophyseal-thyroid axis and the impact of refeeding in anorectics are limited. the sample consistent of 15 patients with anorexia nervosa before and 1 month after partial weight recovery, and 15 age-matched control subjects. the body mass index (bmi), ...	2000	10921449
effects of thyroxine on rat brown fat and muscle thermogenesis in the cold.	we studied whether the activation of rat brown adipose tissue (bat) by cold exposure or by the administration of beta-3-noradrenergic agonist cgp-12177 could be prevented by the inhibition of thyroxine (t4) to triiodothyronine (t3) conversion. hypothyroid rats were treated with replacement doses of t4, t4 plus iopanoic acid (ia) or t3. groups of rats were placed at 4 degrees c for 24 h or kept at room temperature. cold exposure induced a significant increase in guanosine diphosphate (gdp) bindin ...	2000	10921450
gemcitabine plus vinorelbine as first-line chemotherapy in advanced nonsmall cell lung carcinoma a phase ii trial.	response and survival in patients with advanced or metastatic nonsmall cell lung carcinoma (nsclc) remain poor. as single agents, the nucleoside analog gemcitabine, and the semisynthetic vinca alkaloid vinorelbine, have been shown to be effective in nsclc and to have a low toxicity profile.	2000	10951338
pleuropulmonary and cardiovascular consequences of thoracoscopy performed in healthy standing horses.	six healthy, awake, and pharmacologically restrained mature horses were studied in order to define the changes in cardiopulmonary function during and after exploratory thoracoscopy and to determine the presence of postoperative complications occurring 48 hours after thoracoscopy. in a randomised 3 x 3 latin square design with 2 replications, 18 procedures were performed: 6 right (rth) and 6 left thoracoscopies (lth) and 6 sham procedures (sth). prior to each procedure a physical examination and ...	2000	10952375
[thyroid gland nodules registered at the endocrinology department of the medical center in prokuplje].	thyroid nodules are often found in clinical practice. general population prevalence is about 4%, whereas in women it is over 6%. the basic disorder of cell kinetics of thyroid tumors is inappropriate growth response to corresponding thyroid-stimulating hormone (tsh) concentration.	2000	10953554
[quality control in surgery of locally advanced rectal cancer].	the role of surgery in the therapeutic balance of locally advanced rectal neoplasms needs to be redefined in the light of recent reports on the efficacy of radio- and chemotherapy in neoadjuvant treatment.	2000	10953562
[prognostic factors in gastric cancer. our experience and review of the literature].	this review of the latest literature and retrospective analysis of the authors' series aimed to identify the most relevant prognostic factors for gastric cancer.	2000	10953563
[effect of diabetes in the first year after gastric banding].	initial hormonal and metabolic investigation was performed in a group o 51 patients (mean age 41 years and body mass index--bmi 45.1 kg/m2) referred to gastric banding for morbid obesity. patients were followed for one year period after surgery. using multiple linear regression following parameters influencing bmi change were identified. negative influence on bmi reduction: high insulin, presence of diabetes, low t3/t4 ratio. positive influence on bmi reduction high triglycerides level, positive ...	2000	10953636
myelination of prospective large fibres in chicken ventral funiculus.	in mammals, the oligodendrocyte population includes morphological and molecular varieties. we reported previously that an antiserum against the t4-o molecule labels a subgroup of oligodendrocytes related to large myelinated axons in adult chicken white matter. we also reported that t4-o immunoreactive cells first appear in the developing ventral funiculus (vf) at embryonic day (e)15, subsequently increasing rapidly in number. relevant fine structural data for comparison are not available in the ...	2000	11387549
endocrine disruption: thyroid dysfunction in mummichogs (fundulus heteroclitus) from a polluted habitat.	mummichogs (fundulus heteroclitus) from piles creeks (pc), new jersey (a polluted site), are sluggish and show poorer prey capture and predator avoidance than reference fish from tuckerton (tk). the behavioral dysfunction of the pc fish may be associated with thyroid impairment due to endocrine disruption. in this study, we compared thyroid histology and thyroid hormones in the two populations and determined experimentally whether the polluted environment could alter thyroid hormone levels. pc f ...	2000	11460725
[side effects after treatment with a-interferon in children with chronic viral hepatitis].	this paper aimed at a long-term observation on side effects of alpha- ifn treatment in children with chronic viral hepatitis.	2000	11471032
cancer of the esophagogastric junction.	in the western world, there has been an alarming rise in the incidence and prevalence of adenocarcinoma arising at the esophagogastric junction during recent decades. epidemiological, clinical and pathological data support a sub-classification of adenocarcinomas arising in the vicinity of the esophagogastric junction (aeg) into adenocarcinoma of the distal esophagus (type i), true carcinoma of the cardia (type ii) and subcardial carcinoma (type iii). while most, if not all, adenocarcinomas of th ...	2000	11525305
[preoperative staging on liver metastasis from colorectal carcinoma].	preoperative staging of metastatic liver cancer (mlc) from colorectal carcinoma (crc) is conducted with the purpose to identify potentially resectable mlc cases. fifty-two patients undergo laparoscopic study (4.1% all endoscopies), and three--laparoscopic ultrasonography. laparoscope e wolf and 7.5 mhz linear transducer obtained from the aloka company are employed. the study covers 24 women and 28 men with mean age 61.6 years (range 23-87 y). mlc is diagnosed in 35.1% (13/37) of colonic, and in ...	2000	11484258
purification of the gp31 co-chaperonin of bacteriophage t4.		2000	11484492
epidural analgesia attenuates the systemic stress response to upper abdominal surgery: a randomized trial.	the effects of combined general anaesthesia and epidural analgesia in various endocrine and metabolic parameters were studied before, during, at the end, and 72 h after upper abdominal surgery, in an effort to further elucidate the role of epidural analgesia in the endocrine and metabolic response. 50 patients were randomly assigned into groups a and b, which received general anaesthesia alone and combined general anaesthesia and epidural analgesia, respectively. the effects of surgical stress i ...	2000	11589607
serotypes and phage types of vibrio cholerae in mysore.	a total of 565 samples of acute diarrhoeal stools from children received over a period of twelve months from november 1996 to october 1997, yielded 289 (51.15%) isolates of v. cholerae. out of these, 277 (95.85%) were v. cholerae 01 serotype ogawa, 7 (2.42%) were v. cholerae 0139 and 5 (1.73%) were v. cholerae non-o1 non-0139. phage typing of the isolates was carried out by niced calcutta. out of 78 isolates, 69 (88.46%) belong to phage type t27. of the remaining, 3 (3.85%), 3(3.85%), 2(2.56%) a ...	2000	11668944
prevalence of endocrine dysfunction in hiv-infected men.	endocrine dysfunction is a common problem in patients with human immunodeficiency virus infection (hiv). we therefore evaluated the endocrine function in 31 male homosexual hiv-1-infected men: mean age 37 +/- 7.2 years (range 24-52).	2000	11595820
effects of tripcholorolide on inflammatory reaction of mouse alveolar macrophages in vitro.	to observe the effects of tripcholorolide (t4) on inflammatory reaction of mouse alveolar macrophages.	2000	11603300
[results of radical cystectomy for management of invasive bladder cancer with special reference to prognostic factors and quality of life depending on the type of urinary diversion].	bladder cancer is one of the main problems in urology in terms of diagnosis and treatment, due to its high incidence, high recurrence rate, and difficulties in prognosis of its natural history. according to polish epidemiological reports, bladder cancer was the fifth cause of death due to malignant diseases in 1993 (1.2% in females and 4.0% in males). radical cystectomy remained for over forty years the main method of treatment in cases of invasive bladder cancer. improvements in postoperative c ...	2000	11712306
[indications for preoperative laparoscopic ultrasonography].	109 laparoscopic ultrasound examinations are performed for a period of 7 years (1993-1999). laparoscope r. wolf and 7.5 mhz linear probe "aloka" are used. on the basis of the results analysis the following indications for laparoscopic ultrasonography (lus) are recommended: 1. uncertain diagnosis; 2. contradictory results of imaging technics; 3. assessment of tumour's stage; 4. early diagnosis; 5. negative data for liver lesion on laparoscopic examination and established or suspected tumour by im ...	2000	11692932
metabolic functions of microbial nucleoside diphosphate kinases.	this article summarizes research from our laboratory on two aspects of the biochemistry of nucleoside diphosphate kinase from escherichia coli--first, its interactions with several t4 bacteriophage-coded enzymes, as part of a multienzyme complex for deoxyribonucleoside triphosphate biosynthesis. we identify some of the specific interactions and discuss whether the complex is linked physically or functionally with the t4 dna replication machinery, or replisome. second, we discuss phenotypes of an ...	2000	11768309
dna analysis of laryngeal carcinoma cells by flow cytometry: the histoclinical factors and their significance.	in patients suffering from several types of malignant tumours, changes in deoxyribonucleic acid (dna) content are usually associated with poorer survival prognosis. in the present study, dna content and clinical and histopathologic features were analyzed in patients suffering from laryngeal carcinoma, with a view to establishing the crucial prognostic factors. in the 5-year follow-up study, flow cytometry was used to analyze dna content in the paraffin-embedded samples of laryngeal carcinoma tis ...	2000	11770146
[clinical study of astragalus injection plus ligustrazine in protecting myocardial ischemia reperfusion injury].	to investigate the mechanism of astragalus injection plus ligustrazine in preventing the occurrence of myocardial ischemia reperfusion injury (miri) during open heart surgery of cardiopulmonary bypass, and the treating principle of miri in tcm.	2000	11789206
[clinical observation on treatment of thyrotoxic exophthalmos with jiayanxiao plus tapazole].	to study the therapeutic effect of jiayanxiao (jyx) on thyrotoxic exophthalmos.	2000	11789227
prognostic factors in patients with advanced gastric cancer with macroscopic invasion to adjacent organs treated with radical surgery.	background: the prognosis of patients with gastric cancer with invasion to adjacent organs is poor. the prognostic factors of patients with advanced gastric cancer with macroscopic invasion to adjacent organs (t4) who were treated with radical surgery was determined in the present study.methods: a total of 86 consecutive patients with advanced gastric cancer who underwent radical (potentially curable) gastrectomy with combined resection of other organs for macroscopic invasion to adjacent organs ...	2000	11984737
effects of a single venous dose of zinc on thyroid status in healthy individuals and patients with graves' disease.	zinc metabolism may regulate thyroid function acting at trh (thyrotropin-releasing hormone) synthesis, peripheral deiodination of t4 (tetraiodothyronine), and binding of thyroid hormones to nuclear receptors. the aim of this study was to investigate the effect of acute zinc administration on tsh (thyroid-stimulating hormone), ft3 (free triiodothyronine), and ft4 (free tetraiodothyronine) in 10 healthy individuals and 12 hyperthyroid patients with graves' disease. all these individuals were studi ...	2000	18475939
effect of thyroid hormones on the stage-specific pigmentation of the japanese flounder paralichthys olivaceus.	three experiments were carried out to clarify the effect of thyroid hormones on the pigmentation of larval japanese flounder paralichthys olivaceus. the first two experiments were conducted to investigate the critical concentration of thyroxine (t4) which causes abnormal pigmentation, and the third was to determine the sensitive stage of larval development at which albinism is induced by exogenous t4. the larvae treated with over 10 nm t4 showed a significant increase of albinism in experiments ...	2000	18522465
[persistent hyperinsulinemic hypoglycemia of infancy: case report]	objective: to report a case of persistent hyperinsulinemic hypoglycemia in twins which is a situation not yet reported in the literature. methods: report of seizures in identical twins, from consanguineous parents, with persistent hypoglycemia as cause of the seizures. laboratory tests, performed for etiological investigation of the hypoglycemia, included thyroid hormones (t4/tsh), insulin, cortisol, growth hormone, stimulation test with glucagon (to evaluate the insulin/glucose relation), and h ...	2000	14647692
[tetanus osteoarticular complications in dakar].	to assess the epidemiological and clinical features of osteoarticular symptoms during tetanus infection, we carried out a retrospective study in infectious diseases ward of the university teaching hospital in dakar (senegal). over a period of 7 years (1990-1996), 1, 199 cases of tetanus of more than 1 month of age were diagnosed. among those who survived (n = 948), 18 cases of osteo-articular manifestations were observed (1.8%): 15 presented with vertebral fracture located within the thoracical ...	2000	14666790
[study on midazolam used for awake blind intubation of maxillofacial surgery].	to provide clinical data for midazolam to be used as an assistant drug for awake tracheal intubation.	2000	15014819
gastroesophageal junction adenocarcinoma.	the incidence rate of adenocarcinoma of the esophagogastric junction (aeg) is increasing in association with the epidemiologic rise in distal esophageal adenocarcinoma and gastric cardial (aeg type iii) tumors. the overall survival rate is poor in most patients with aeg because lymph node or visceral metastases are frequently present at the time patients become symptomatic. a few patients are identified early in the disease because of screening for gastroesophageal reflux and barrett's esophagus ...	2000	12057146
[in carciraded inguinoscrotal hernia within sigmodial perforated adenocarcinoma and peritonitis in the scrotal sac.a case report]	a case of perforated sigmoid colon, complicated with peritonitis inside the inguinal hernia scrotal sack is presented. a 91 years old male with reducible inguinal tumor from 4 years ago, that 4 days before hospitalization turns to an unyielding restitution inguinal hernia. producing an intestinal obstruction. sigmoidectomy with a hartman colostomy and inguinal herniorraphy was done. anatomopatologycal study show adenocarcinoma of colon, tubular type, with moderate differentation. tnm classificat ...	2000	12140589
the application of a novel lytic system to the recovery of recombinant proteins in e.coli.	in order to efficiently recover recombinant proteins, a temperature-sensitive lytic system was constructed on the basis of the feature that t4 lysozyme disrupts the bacteria through cutting specific bond in the peptidoglycan layer of cell wall. this system was evaluated by constructing and introducing a low copy plasmid psc-lys (psc101 replication origin) into e.coli. the plasmid contained a temperature sensitive t4 lysozyme (lys(ts)) gene under the control of three tandem tac promoters and the ...	2000	12075442
hiv vaccine development and africa.	the genetic variation of hiv poses a great problem in the development of a vaccine that will work against the viral subtypes that predominate in africa. hiv-1 exists in as many as 10 subtypes and these subtypes have 20-30% inter-subtype variation, while differences within a subtype can differ up to 15%. moreover, hiv differs from person to person; it creates so many different versions of itself, which overwhelms the person's immune system. however, many areas of the code are conserved or shar ...	2000	12322293
the role of pretreatment serum albumin to predict pathological stage and recurrence among radical prostatectomy cases.	serum albumin level has been implicated as a prognostic factor in various cancers, however it has not been studied in early stage prostate cancer. we examine the clinical prognostic value of the readily available pretreatment albumin level as a marker for determining staging and prognosis for men undergoing radical prostatectomy. a retrospective review was performed on 354 patients who underwent a radical prostatectomy at a tertiary facility from 15 april, 1990 until 3 december, 1996 who had a p ...	2000	12497095
[effect of haidonghua powder(hdhp) on hypothalamic obesity in rats].	to study the effect of hdhp including laminaria japonica aresch. and benincasa hispida(thunb.) cogn. etc. on antiobesity in rats with hypothalamic obesity.	2000	12515214
[therapy for short stature in girls with turner syndrome].	this study was aimed to examine the growth-promoting effects of recombinant human growth hormone (r-hgh) and low dose of thyroid hormone on short stature in girls with turner syndrome. 8 girls with turner syndrome, at the chronological age of 8.5-14.5 years (12.3 +/- 2.1 years) and bone age of 8.3-12.7 years (9.8 +/- 1.7 years) were observed. the maximum serum gh levels in 3 patients out of 5 with provocative testing available were > or = 10 ng/ml (10.0, 10.0 and 15.52 ng/ml, respectively), and ...	2000	12545844
neonatal congenital hypothyroidism screening in addis ababa, ethiopia.	to determine the prevalence of congenital hypothyroidism, establish reference levels for thyroid stimulating hormone (tsh), and determine the cut-off points of tsh level for neonatal screening in congenital hypothyroidism.	2000	12862157
stereological analysis of the cerebellum of 24-day old rats with propylthiouracil-induced hypothyroidism.	to investigate the structural parameters of the developing cerebellum in propylthiouracil (ptu)-induced hypothyroidism during pre and postnatal stages in 24 day old rat pups.	2000	12858908
fluorescence microscopic visualization of a dna-cationic fullerene complex.	cationic fullerene derivative 1, which is soluble in aqueous medium, bound to double stranded dna. observation of coliphage t4 dna in the absence and presence of 1 by fluorescence microscopy suggested that 1 bound to dna along its groove. upon irradiation of visible light on the dna complex of 1, dna was cleaved dramatically. the process of dna photo-cleavage could be monitored continuously by fluorescence microscopy.	2000	12903324
preparation of dna catenanes and observation of their topological structures by atomic force microscopy.	dna catenanes have been prepared by the reaction of t4 dna ligase with linear dna in the presence of nicked dna. single molecular images of dna catenanes and large circular dnas have been clearly observed by afm using a tapping mode at room temperature and in an ambient atmosphere.	2000	12903352
single long dna molecule analysis using fluorescence microscopy.	single long dna molecule (t4 dna) in agarose gel was visualized with a fluorescence microscope. we confirmed alternating current electric fields is effective for stretching of single dna molecule in agarose gel. this stretching phenomenon was observed with wide range of agarose gel concentration from 0.5%(w/v) to 1.5%. from this observation, the presence of agarose gel fiber is essential for this stretching phenomenon. the stretching process of several dna molecules in gel shows discontinuity, w ...	2000	12903361
salt-dependent folding of long duplex dna by histone h1.	it is found that t4 phage dna complexed with histone h1 assembled into a string-of-bead structure, when the complex is prepared by a gentle diluting procedure from a high salt solution (2 m nacl) to a low salt solution (50 mm nacl). we used fluorescence microscopy to perform the real-time observation on formation and motion of a string-of-bead structure. spatial histone h1 distribution on the dna-h1 complex is observed by immuno-fluorescence microscopy.	2000	12903384
[effect of tripterygium wilfordii hook t4 monomer on proliferation and interleukin-6 production of synovial fibroblasts of patients with rheumatoid arthritis].	tripterygium wilfordii hook multi-glycosides t2 has been wildly used in china in treatment of ra. t4 was abstracted from t2 and was reported much more efficient in anti-inflammatory and immune suppression than t2. this study was to investigate the effect tripterygium wilfordii hook t4 monomer on proliferation and interleukin-6 production of synovial fibroblasts of patients with rheumatoid arthritis.	2000	12903526
[horizontal-vertical hemilaryngectomy for advanced laryngeal cancer].	to evaluate the adequacy of horizontal-vertical partial laryngectomy in the treatment of advanced laryngeal cancer.	2000	12768673
[laryngeal function-sparing surgery for pyriform sinus cancer].	to evaluate the oncologic effect of laryngeal function-sparing surgery for the patients with pyriform sinus carcinoma.	2000	12768706
[measuring disability of patients with low-back pain--validation of a german version of the roland & morris disability questionnaire].	in this study three instruments measuring disability of patients with low-back pain are presented and evaluated: (1) the behinderungsfragebogen (rm) - a german version of the roland & morris disability questionnaire (rdq) (2) a numerical rating scale measuring disability in general and (2) eight numerical rating scales measuring specific dimensions of disability (standing, sitting, walking, driving a car, carrying light loads, carrying heavy loads, sleeping, and sexual intercourse).	2000	12800012
[long-term follow-up result of partial laryngectomy].	to study the long-term follow-up result of partial laryngectomy and reservation of laryngeal function.	2000	12768742
enzymic chemical reaction under microgravity environment in space.	in recent years, some papers have reported synergism in the biological effects of space radiation and microgravity. however, there is no direct evidence for these phenomena. as one possible mechanism, we investigated whether dna ligation in the final step of dsbs repair of dna molecules induced by radiation is depressed by microgravity. therefore, we have scheduled the space experiments of the effects of microgravity on repair activity of t4 dna ligase for dsbs prepared with digestion of a restr ...	2000	12697569
[thyrotoxicosis and coexistent diseases in atrial fibrillation.]	objective: to evaluate the prevalence of thyrotoxicosis and coexistent diseases in patients with atrial fibrillation admitted to a general medicine ward. material and methods: all patients with the discharge diagnosis of atrial fibrillation were studied. the study design was retrospective for the years 1993-1994 and prospective from april 1995 through december 1997. information on thyroid function tests was obtained from medical records in the retrospective part, but tsh, t4 and t3 were measured ...	2000	17018961
effects of cardiopulmonary bypass on thyroid function.	alterations in serum concentration of thyroid hormones occur even in euthyroid patients undergoing cardiopulmonary bypass. the purpose of our prospective study was to define the effects of cardiopulmonary bypass on thyroid hormones. twenty euthyroid patients for mitral valve replacement were included in this study. heparinised arterial sample for thyroid function tests were obtained before cardiopulmonary bypass (cpb), 30 and 60 minutes after initiation of cpb, immediate post-cpb and 24 hours af ...	2000	17848764
evidence-based outcomes for patients with non-small cell lung cancer undergoing resection.	surgery plays a vital role in the management of stages i-iiia nsclc. careful patient selection and improved perioperative care now allow pulmonary resection to be performed with a very low morbidity and mortality even in older patients, or in patients undergoing pneumonectomy or extended operations. most patients with stage iiib disease are not candidates for surgery, but resection can be curative for highly selected groups of patients with t4 tumors when these are not associated with nodal meta ...	2000	16088754
characterization of oxygen-dependent lysis of escherichia coli cells infected by bacteriophage t4.	infection of escherichia coli cells by t4 phage at a multiplicity of infection (moi) of 0.01 caused inhibition of cell lysis for up to 4 h. such cells grown under aerobic condition were lysed by external stimuli such as cold shock, osmotic shock or addition of toxic substances, e.g., carbonyl cyanide m-chlorophenylhydrazone (cccp). however, the effects of these external stimuli were reduced by transferring the cells to static incubation, by which dissolved oxygen was consumed by the cells within ...	2000	16232751
[transient neonatal hypothyrosis in premature neonates].	plasma levels were measured of t4, free t4, t3, tsh in 64 premature neonates and in 41 those born at right time during the time-related course of the neonatal period. the results secured permit regarding the thyroid hypohormonosis syndrome in premature neonates as a consequence of the hypothalamic-hypophyseal-thyroid axis deficiency and of immaturity of the thyreostatic receptors as well. on the one hand, this promotes hypometabolism and, correspondingly, favours more economic utilization of bod ...	2000	16786655
[association of atypical pulmonary tb, polyserositis, severe leukopenia and panniculitis. case report].	many reports have associated tuberculosis with haematological abnormalities. these reports suggest that severe pulmonary tuberculosis, if associated with reduced tissue cellular reaction, may cause blood discrasias. anemia was present in 32 percent of patients. leucopenia with neutropenia and lymphopenia was observed in 15 percent in patients with very severe clinical tuberculosis. active tuberculosis was associated with significant reductions in absolute numbers of total t, t4 and b lymphocytes ...	2001	11977490
[surgical treatment of neck lymph nodes in squamous cell carcinoma of the pyriform sinus].	the present study reports the results of 66 patients surgically treated for squamous cell carcinoma of the pyriform sinus between 1984 and 1996. twenty eight patients underwent mono!ateral neck dissection and bilateral neck dissection was performed in 38 cases, for a total of 104 radical neck dissections. of these, 73 (71%) were modified type iii dissections, 17 (16%) were classical, and 14 (13%) were modified type i and ii dissections. the primary lesion was strictly lateralized in 47 cases (71 ...	2001	11938706
a simple way to reduce heat stress in laying hens as judged by egg laying, body weight gain and biochemical parameters.	this study was designed to test the effects of feed withdrawal and darkening on the performance, triiodothyronine (t3), thyroxine (t4), thyroid-stimulating hormone (tsh), adrenocorticotropic hormone (acth), and some blood serum metabolite and mineral concentrations of laying hens reared at high ambient temperatures ranging from 25 to 35 degrees c. ninety, 16-week-old hens (ross brown) were divided into 3 groups, 30 hens each. the first group was used as control. hens in the second group (feed wi ...	2001	11942121
low dose preoperative radiation in the treatment of carcinoma of the rectum.	recent advances in the treatment of cancer of the rectum show a steady improvement in survival. newer surgical techniques and expanding options for adjunctive therapy appear to have had a significant impact on improving both local control and distant disease. five-year survival of patients now ranges from 85-90% for stage i cancers and 50-55% for stage iii cancers. local recurrence of disease following curative surgical resections is dependent on the stage of the tumor and for some high-risk pat ...	2001	11975407
thyroid cancer in children and adolescents--consequences in later life.	thyroid cancer is rare below the age of 16 years, with an annual incidence of 0.02-0.3 cases per 100,000. papillary and follicular thyroid cancer in childhood and adolescence is more advanced upon presentation than in adults, as evident from a higher frequency of extra-thyroidal spread. the recurrence rate is also higher. nevertheless, the prognosis for survival in children and adolescents is better than in adults; why this is so remains unclear. an approximately 30-fold increase in the incidenc ...	2001	11964025
chemoenzymatic synthesis of biotinylated nucleotide sugars as substrates for glycosyltransferases.	the enzymatic oxidation of uridine 5'-diphospho-alpha-d-galactose (udp-gal) and uridine 5'-diphospho-n-acetyl-alpha-d-galactosamine (udp-galnac) with galactose oxidase was combined with a chemical biotinylation step involving biotin-epsilon-amidocaproylhydrazide in a one-pot synthesis. the novel nucleotide sugar derivatives uridine 5'-diphospho-6-biotin-epsilon-amidocaproylhydrazino-alpha-d-galactose (udp-6-biotinyl-gal) and uridine 5'-diphospho-6-biotin-epsilon-amidocaproylhydrazino-n-acetyl-al ...	2001	11948877
chemotherapy with vinorelbine, cisplatin and continuous infusion of 5-fluorouracil in locally advanced breast cancer: a promising low-toxic regimen.	primary chemotherapy for locally advanced breast cancer, usually an anthracycline-containing regimen, improves local disease control allowing for an initially inoperable tumour to be resected. the feasibility and efficacy of a regimen containing vinorelbine (v), cisplatin (p) and 5-fluorouracil (5-fu) as continuous infusion (vifup regimen) for patients with locally advanced breast cancer were evaluated. twenty-six patients with a t4 breast cancer presentation (eight also had synchronous distant ...	2001	11911307