Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| peptide inhibitors of dna cleavage by tyrosine recombinases and topoisomerases. | the study of biochemical pathways requires the isolation and characterization of each and every intermediate in the pathway. for the site-specific recombination reactions catalyzed by the bacteriophage lambda tyrosine recombinase integrase (int), this has been difficult because of the high level of efficiency of the reaction, the highly reversible nature of certain reaction steps, and the lack of requirements for high-energy cofactors or metals. by screening synthetic peptide combinatorial libra ... | 2000 | 10873446 |
| evaluation of enzymatic mutation detectiontrade mark in hereditary nonpolyposis colorectal cancer. | in hereditary nonpolyposis colorectal cancer (hnpcc), the majority of reported mutations are dispersed throughout the 35 exons of the two principal susceptibility genes, mlh1 and msh2, and because of this complexity, rapid mutation screening methods are required. the aim of this study was to evaluate the sensitivity of the enzymatic mutation detection (emd) assay in hnpcc using genomic dna samples with known gene alterations in mlh1 and msh2. the emd assay relies upon the enzyme t4 endonuclease ... | 2000 | 10874307 |
| enzymatic mutation detection (emd) of novel mutations (r565x and r1523x) in the fbn1 gene of patients with marfan syndrome using t4 endonuclease vii. | the enzymatic mutation detection (emdtrade mark) method is a streamlined and improved version of the original enzymatic cleavage of mismatch (emc) method. emd is a fully homogeneous, rapid four step procedure that allows for detection and localization of mismatched or unmatched nucleotides within heteroduplex dna. to test the utility of emd for use in the screening of large and complex genes, the fibrillin 1 (fbn1) gene was scanned in a cohort of six patients diagnosed with connective tissue dis ... | 2000 | 10874320 |
| natural history and patterns of recurrence of tongue tumours. | this retrospective study comprises 226 patients with squamous cell carcinoma of the tongue treated at canniesburn hospital, glasgow between 1980 and 1996. the male:female ratio was 1.2:1 and the average age was 64 years. the ratio of anterior 2/3 to posterior 1/3 tongue lesions was 1.8:1. a total of 23% of patients were clinically staged as t1, 50% were t2 and 27% were t3/t4. at presentation 156 patients (69%) had a clinically negative neck, while 110 patients (49%) had a neck dissection at the ... | 2000 | 10876250 |
| influence of whitacre spinal needle orifice direction on the level of sensory blockade. | the purposes of the present study were to determine if the direction of the needle orifice during injection of anesthetic into the subarachnoid space, using a 25-gauge whitacre spinal needle (becton dickinson, franklin lakes, nj) to deliver 15 mg of 0.75% hyperbaric bupivacaine, affected the level of sensory blockade achieved, and if there was a difference in time from injection to surgical anesthesia based on needle orifice direction. a convenience sample was selected from patients presenting f ... | 2000 | 10876454 |
| concordant decreases of thyroxine and thyroxine binding protein concentrations during sepsis. | the principal thyroxine (t4) binding proteins were measured in 8 septic patients and 8 controls to determine the extent to which a decrease in their concentration contributes to the decrease in serum t4 in sepsis. t4 binding globulin (tbg) evaluated by radioimmunoassay (ria) and radial immunodiffusion (rid) was 61% and 66%, respectively, of the normal mean value in sera from septic patients. decreases of albumin and transthyretin (ttr) to 55% and 29%, respectively, of the normal mean concentrati ... | 2000 | 10877201 |
| gene regulation by thyroid hormone. | regulation of gene expression by thyroid hormones (t3, t4) is mediated via thyroid hormone receptors (trs). trs are dna-binding transcription factors that function as molecular switches in response to ligand. trs can activate or repress gene transcription depending on the promoter context and ligand-binding status. in most cases, in the absence of ligand, trs interact with a corepressor complex containing histone deacetylase activity, which actively inhibits transcription. the binding of ligand ... | 2000 | 10878749 |
| thyroid hormone resistance and increased metabolic rate in the rxr-gamma-deficient mouse. | vitamin a and retinoids affect pituitary-thyroid function through suppression of serum thyroid-stimulating hormone (tsh) levels and tsh-beta subunit gene expression. we have previously shown that retinoid x receptor-selective (rxr-selective) ligands can suppress serum tsh levels in vivo and tsh-beta promoter activity in vitro. the rxr-gamma isotype has limited tissue distribution that includes the thyrotrope cells of the anterior pituitary gland. in this study, we have performed a detailed analy ... | 2000 | 10880050 |
| traumatic medial malleolar fracture of a fibula flap donor leg. | a 50-year-old male presented with a t4 n0 squamous cell carcinoma of the floor of the mouth and alveolus. treatment included a partial mandibulectomy with a free osseocutaneous fibula flap reconstruction. he made a good postoperative recovery and was given adjuvant radiotherapy. no problems were reported with the donor site or ankle. five months after harvesting the flap the patient sustained a fracture of the medial malleolus having jumped over a ditch playing golf. this was treated successfull ... | 2000 | 10880172 |
| relationship between serum leptin and thyroid hormones in children. | because leptin decreases food intake and increases energy expenditure, the possible influence of thyroid status on the leptin system has been investigated mainly in adults and animals. however, the data available at present are very confusing. the aim of the present study was to assess the possible interaction of thyroid hormones with the leptin system. | 2000 | 10881589 |
| [thyroid function tests in normal pregnant women (third trimester) and in pregnant women with pregnancy cholestasis or with acute hepatitis]. | intrahepatic cholestasis of pregnancy (icp) is a disease of unknown cause characterized by pruritus and biochemical cholestasis in the 3rd trimester of pregnancy. its pathogenesis may be due to the interaction of abnormalities in the metabolism of estrogens and progesterone, while still unknown environmental factor (s) modulate the expressivity of a genetic predisposing trait. | 2000 | 10883520 |
| u-shaped free jejunal transplantation: improved technique and indications. | reconstruction of the oropharynx and the hypopharynx is difficult due to their wide diameters. we report eight cases of circumferential pharyngolaryngectomies for epidermoid carcinomas of the hypopharynx extended to the oropharynx and classified t4. reconstruction was performed with a u-shaped free jejunal transplantation. this specific technique consists of a side to side anastomosis between two loops of jejunum. it facilitates reconstruction at the level of the oropharynx and diminishes postop ... | 2000 | 10883824 |
| hypothyroxinemia of prematurity and infant neurodevelopment: a pilot study. | to assess whether hypothyroxinemia has specific effects on neurodevelopment in premature infants, thyroid hormone levels were determined at 2 weeks of life and 40 weeks postconceptional age (pca), and infants were evaluated at 3 months corrected age using the bayley scales of infant development and early infancy temperament questionnaire. additional attention scales were derived from the factor analysis of relevant bayley items. fifteen infants born between 30 and 35 weeks and 21 full-term infan ... | 2000 | 10883877 |
| congenital central isolated hypothyroidism caused by a homozygous mutation in the tsh-beta subunit gene. | we report a belgian girl born in 1983 with isolated thyrotropin (tsh) deficiency. hypothyroidism without goiter was diagnosed at the age of 2 months, with extremely low total thyroxine (t4) at 0.3 microg/dl (4 nmol/l; n[normal]: 5.6-11.4 microg/dl). basal tsh, only moderately elevated at 14.8 mu/l (n: 0-5.3; competitive radioimmunoassay, ria), increased to 18.2 mu/l after thyrotropin-releasing hormone (trh) stimulation, whereas prolactin increased normally. at age 15 years, after withdrawal of l ... | 2000 | 10884185 |
| neuronal structure of microvascular transplants with and without neuronal anastomosis. | latissimus dorsi transplants have little neuronal regenerative capacity without neuronal anastomosis. histologic differences between transplants with and without neuronal anastomosis and 2 distinct types of neurosurgical reanastomosis are highlighted in this study. | 2000 | 10884632 |
| traumatic medial malleolar fracture of a fibula flap donor leg. | a 50-year-old male presented with a t4 n0 squamous cell carcinoma of the floor of the mouth and alveolus. treatment included a partial mandibulectomy with a free osteocutaneous fibula flap reconstruction. he made a good postoperative recovery and was given adjuvant radiotherapy. no problems were reported with the donor site or ankle. five months after harvesting the flap the patient sustained a fracture of the medial malleolus having jumped over a ditch playing golf. this was treated successfull ... | 2000 | 10885815 |
| lymphocyte subset abnormalities in multitransfused hiv-negative haemophilia a patients are not due to chronic hepatitis c virus infection. | several abnormalities of immune parameters have been described in hiv-negative haemophiliacs, including changes in numbers of t4 and t8 cells, t4/t8 ratio and numbers of activated t cells. to assess the contribution of hepatitis c to these abnormalities, we compared lymphocyte subsets in 20 hiv-negative hcv-positive haemophilia a patients and in 14 non-haemophiliacs with chronic hepatitis c with those in 18 healthy controls. in haemophilia patients, higher numbers of t lymphocytes and activated ... | 2000 | 10886199 |
| incidentally detected liver metastasis of well-differentiated follicular carcinoma of the thyroid, mimicking ectopic thyroid. | a case of incidentally detected liver metastasis of follicular carcinoma of the thyroid, histologically mimicking ectopic thyroid, is described. the patient was a 48-year-old woman. a 2-cm mass was incidentally detected in the left lobe of the liver by abdominal computed tomography (ct) scan. partial liver resection was performed for diagnosis and treatment. histologically, the liver nodule was composed of small-to-large follicles containing colloid material. the lining epithelium was flat or cu ... | 2000 | 10886730 |
| the effects of microgravity on ligase activity in the repair of dna double-strand breaks. | in recent years, contradictory data have been reported about the effects of microgravity on radiation-induced biological responses in space experiments. the aim of the present study was to clarify whether enzymatic repair of dna double-strand breaks is affected by microgravity using an in vitro enzymatic reaction system. | 2000 | 10902732 |
| management of the solitary thyroid nodule: results of a north american survey. | the present survey evaluated current trends in the management of the nontoxic solitary thyroid nodule by expert endocrinologists in north america and compared their results with a similar european thyroid association survey. a questionnaire was circulated to all clinical members of the american thyroid association. an index case (a 42-yr-old woman with a solitary 2 x 3-cm thyroid nodule and no clinical suspicion of malignancy) and 11 variations were provided to evaluate how each alteration would ... | 2000 | 10902799 |
| results of combined resection of adjacent organs in lung cancer. | indications for surgical treatment in advanced lung cancer still remain to be established. | 2000 | 10903057 |
| a statistical appraisal of native state hydrogen exchange data: evidence for a burst phase continuum? | for a number of proteins, folding occurs via the rapid accumulation of secondary and tertiary structural features in a so-called burst phase, preceding the relatively slow, highly activated transition leading to the native state. a fundamental question is: do these burst phase reactions comprise two phase-separated thermodynamic states or a continuum of states? ribonuclease hi (rnase h) from escherichia coli and phage t4 lysozyme (t4l) both exhibit such a phenomenon. native-state hydrogen exchan ... | 2000 | 10903874 |
| effect of hibernation, thyroid hormones and dexamethasone on cytosolic and mitochondrial glycerol-3-phosphate dehydrogenase from jerboa (jaculus orientalis). | tissue distribution of the cytosolic and mitochondrial glycerol-3-phosphate dehydrogenase (cgpdh and mgpdh) activities in jerboa (jaculus orientalis), a hibernator, shows the highest level of enzyme activity in skeletal muscle and brown adipose tissue, respectively. the effect of hibernation on cgpdh indicates an increase of activity in all tissues examined. in contrast, hibernation decreases mgpdh activity in all tissues, except skeletal muscle. the effect of thyroid hormones on gpdh activity w ... | 2000 | 10904857 |
| mutations in the n-terminal cooperativity domain of gene 32 protein alter properties of the t4 dna replication and recombination systems. | the gene 32 protein (gp32) of bacteriophage t4 is the essential single-stranded dna (ssdna)-binding protein required for phage dna replication and recombination. gp32 binds ssdna with high affinity and cooperativity, forming contiguous clusters that optimally configure the ssdna for recognition by dna polymerase or recombination enzymes. the precise roles of gp32 affinity and cooperativity in promoting replication and recombination have yet to be defined, however. previous work established that ... | 2000 | 10906124 |
| extracorporeal photochemotherapy alone or with adjuvant therapy in the treatment of cutaneous t-cell lymphoma: a 9-year retrospective study at a single institution. | extracorporeal photochemotherapy (ecp; photopheresis) is a treatment option for cutaneous t-cell lymphoma (ctcl). | 2000 | 10906649 |
| architecture of the replication fork stalled at the 3' end of yeast ribosomal genes. | every unit of the rrna gene cluster of saccharomyces cerevisiae contains a unique site, termed the replication fork barrier (rfb), where progressing replication forks are stalled in a polar manner. in this work, we determined the positions of the nascent strands at the rfb at nucleotide resolution. within an hpai-hindiii fragment essential for the rfb, a major and two closely spaced minor arrest sites were found. in the majority of molecules, the stalled lagging strand was completely processed a ... | 2000 | 10891513 |
| antithyroid action of tamoxifen in the rat: in vivo and in vitro studies. | the influence of administration of tamoxifen (tam) on thyroid metabolism was investigated. the potential action of tam on iodine in the thyroid gland was evaluated by determination of the equilibrium constant of the charge transfer complex formed with molecular iodine and by computational studies. adverse effects of tam on thyroid function parameters were also investigated in female wistar rats. rats were treated for seven weeks with 5 mg/kg/day of tam. irrespective of the iodine content of the ... | 2000 | 10895077 |
| epstein-barr virus latent membrane protein 1 (ebv-lmp1) and tumor proliferation rate as predictive factors of nasopharyngeal cancer (npc) radiation response. | irradiation is still the treatment of choice in npc treatment as one of highest malignancy in indonesia as well as in southeast asia. up to now there is no accurate predictor on radiation response, since that the similar histo-morphological pattern, as a well-known prognostic factor can revealed a wide range of treatment outcomes. purpose of the study is to established the influence of ebv-lmp 1 as the most important protein expressed by ebv oncogenes in cellular behaviour such as proliferation ... | 2000 | 10895175 |
| [prognostic factors in non-hodgkin's lymphoma of waldeyer's ring and the lymph nodes of the neck]. | prognostic factors and treatment outcome of 74 patients with non-hodgkin's lymphoma of waldeyer's ring (male 34, female 16, median age 63 years) and cervical lymph nodes (male 17, female 7, median age 62 years) were analyzed retrospectively. the prognostic factors analyzed were: age, b-symptoms, serum ldh levels, and ann arbor stage. we also analysed the tnm classification (1987) of waldeyer's ring lymphoma (previously referred as nasopharyngeal and oropharyngeal cancer) as a new prognostic fact ... | 2000 | 10897589 |
| [intensified after-care--a method for improving occupational reintegration after myocardial infarct and/or bypass operation]. | occupational reintegration after coronary heart disease of patients who are insured by the german workers pension (arbeiterrentenversicherung) succeeds in 40-60% of the recorded cases. patients who were not able to return to work after finishing their regular cardiac rehabilitation took part in a program called "intensivierte nachsorge (ina)". ina is an interdisciplinary support program, taking place twice a week for a whole day over a period of six weeks. on the remaining three days patients we ... | 2000 | 10900673 |
| [parameters of lipid metabolism during perimenopause and thyroid pathology]. | a total of 210 perimenopausal women were examined. lipid status was evaluated in groups of patients differing by thyroid function and characteristics of the perimenopausal period. case histories were analyzed using a universal information chart. serum levels of t3, t4, tth, lh, fsh, prl, e2, and progesterone were radioimmunoassayed. blood lipid spectrum was evaluated on a kone device (finland) by special standard methods. high levels of cholesterol and triglycerides, a tendency to an increase in ... | 2000 | 10900853 |
| the influence of thyroxine on intensity of energy metabolism in bone marrow myeloid cells and neutrophilic polymorphonuclear leukocytes of neonatal pig. | to investigate the participation of thyroxine in the regulation of energy metabolism in neutrophilic polymorphonuclear leukocytes and their bone marrow precursors. | 2000 | 10911408 |
| iodine deficiency and increased risk from radioactive iodine intake in nuclear medicine personnel. | since the entity of the stable endogenous iodine pool is inversely related to the probability of thyroid uptake of radioactive iodine isotopes in subjects exposed for professional reasons to radioiodine intake, we evaluated the urinary iodine excretion, which is a reliable indicator of stable iodine daily intake, in 19 subjects of a nuclear medicine department. seventeen out of 19 subjects showed a reduced iodine elimination < 150 mcg iodine per g of urinary creatinine, while the values of tsh a ... | 2000 | 10911555 |
| squamous cell carcinoma of the external auditory canal: an evaluation of a staging system. | the study was conducted to review a staging system proposed by the university of pittsburgh for temporal bone cancer and to evaluate survival status according to stage, treatment, and certain prognostic factors. | 2000 | 10912706 |
| crystal structures of spin labeled t4 lysozyme mutants: implications for the interpretation of epr spectra in terms of structure. | high resolution (1.43-1.8 a) crystal structures and the corresponding electron paramagnetic resonance (epr) spectra were determined for t4 lysozyme derivatives with a disulfide-linked nitroxide side chain [-ch(2)-s-s-ch(2)-(3-[2,2,5,5-tetramethyl pyrroline-1-oxyl]) identical with r1] substituted at solvent-exposed helix surface sites (lys65, arg80, arg119) or a tertiary contact site (val75). in each case, electron density is clearly resolved for the disulfide group, revealing distinct rotamers o ... | 2000 | 10913245 |
| increased prevalence of thyroglobulin antibodies in sri lankan schoolgirls--is iodine the cause? | iodine deficiency was the likely cause of a high prevalence of goitre previously in sri lankan schoolchildren. salt iodination was made compulsory in 1993 but there has been no recent study, using modern techniques, of its benefits or harmful effects. | 2000 | 10913936 |
| the in vivo effects of beta-3-receptor agonist cgp-12177 on thyroxine deiodination in cold-exposed, sympathectomized rat brown fat. | the effects of the beta-3-receptor agonist cgp-12177 on thyroxine (t4) deiodination in sympathectomized (sx) interscapular brown adipose tissue (bat) were assessed in 300 g body weight (bw) wistar rats. | 2000 | 10913948 |
| comparison of 99mtc-methoxyisobutyl isonitrile and 201t1 scintigraphy in visualization of suppressed thyroid tissue. | both (201)ti and (99m)tc-methoxyisobutyl isonitrile (mibi) have been used in the visualization of suppressed thyroid tissue in patients with autonomously functioning thyroid nodules (aftns). it has been suggested that thyroid-stimulating hormone (tsh) control is not a major determinant of both tracers. however, the mechanism of thyroid uptake of these agents is controversial. in this study, we compared (201)ti and mibi in the visualization of suppressed thyroid tissue in patients with a solitary ... | 2000 | 10914905 |
| functional lip reconstruction with a radial forearm free flap combined with a masseter muscle transfer after wide total excision of the chin. | total lower lip reconstruction was accomplished by combining a radial forearm free flap with a masseter muscle transfer. the patient, who had t4 carcinoma, had the entire lower lip resected including the depressor anguli oris muscle. a radial forearm flap was used to reconstruct the lower lip lining and the floor of the oral cavity. the right and left masseter musculofascial flaps were elevated and transferred in the medial-superior direction, and the peripheral margins of the flaps were sutured ... | 2000 | 10917103 |
| surgical treatment and postoperative outcomes for middle and lower bile duct carcinoma in japan--experience of a single institute. | recently, the japanese classification on cancer of the biliary tract was revised and adopted the new comprehensive staging that is similar to uicc's tnm classification. we should be paying close attention to the significance of newly defined extensive factors of carcinomas on the long-term prognosis. | 2000 | 10919005 |
| video-endoscopic ultrasonography in staging gastric carcinoma. | clinical staging of gastric carcinoma is important in designing the strategy of treatment. early gastric carcinomas can be treated by minimally invasive therapy, whereas advanced gastric carcinomas should be treated by surgery with or without combined chemotherapy. this study was undertaken to evaluate the accuracy and limitations of video type endoscopic ultrasound in preoperative staging of gastric cancer and assessing lymph node metastasis. | 2000 | 10919057 |
| effect of feeding level and thyroxine on adipose tissue development and growth in postnatal lambs. | we investigated the influence of exogenous thyroxine (t4) administration in conjunction with level of feeding on adipose tissue and liver growth in postnatal lambs. pairs of lambs were fed either 100 g (i.e. low fed) or 200 g (i.e. high fed) of milk powder per litre of reconstituted milk replacer over the first month of life. half of the pairs of lambs were fed a bolus dose of t4 (15 mg (kg body weight)-1) daily until 8 days of age. perirenal adipose tissue and hepatic tissue were sampled at eit ... | 2000 | 10919873 |
| differences in bone turnover and skeletal response to thyroid hormone treatment between estrogen-depleted and repleted rats. | this study was undertaken to compare the effect of supraphysiological doses of thyroxine (t4) on bone metabolism in sham and ovx young adult rats. female sprague dawley rats (220 +/- 2 g, approx. 5 months of age) were divided into four groups of eight animals each. the animals were intraperitoneally injected 6 days per week with vehicle (vh): 0.001 n naoh/0.9% nacl (sham+vh and ovx+vh) or 250 microg of thyroxine/kg/day (sham+t4 and ovx+t4) during a 5-week period. serum t4 and osteocalcin (bgp), ... | 2000 | 10920223 |
| quantification of enterovirus rna in sludge samples using single tube real-time rt-pcr. | we have developed a quantitative rt-pcr method that can be used to determine the amount of enterovirus rna in urban sludge samples. this method combines taq-man technology with the abi prism 7700 real-time sequence detection system. we optimized a one-step rt-pcr that uses a dual-labeled fluorogenic probe to quantify the 5' noncoding region of enteroviruses. for accurate quantification of the number of copies, a mahoney type 1 poliovirus rna standard was designed and produced using genetic engin ... | 2000 | 10907082 |
| thyroid hyperactivity induced by methimazole, spironolactone and phenobarbital in marmosets (callithrix jacchus): histopathology, plasma thyroid hormone levels and hepatic t4 metabolism. | to determine drug-induced hyperfunction of marmoset thyroids due to inhibition of synthesis or enhancement of metabolic elimination of thyroid hormones, males were orally administered 10 and 30 mg/kg/day methimazole (mmi), 30 and 100 mg/kg/day spironolactone (spl), or 50 mg/kg/day phenobarbital (pb) for 4 weeks. mmi caused marked hypertrophy of follicular epithelial cells in accordance with a significant decrease in the plasma thyroxin (t4) level. hypertrophied epithelial cells were filled with ... | 2000 | 10907687 |
| thoracic spine dysfunction in upper extremity complex regional pain syndrome type i. | case study. | 2000 | 10907896 |
| the effect of iodine administration on the development of thyroid autoimmunity in patients with nontoxic goiter. | previous studies, mostly performed in iodine-deficient areas, have suggested that the administration of iodine to patients with endemic goiter may be associated with the development of thyroid autoantibodies (thabs); however, this has not been a consistent finding. in this study, we evaluated the effect of iodine on thyroid function and on the development of indices of autoimmunity (thabs and lymphocytic infiltration) in an iodine replete area. | 2000 | 10907993 |
| subacute toxicity of alpha-ergocryptine in sprague-dawley rats. 2: metabolic and hormonal changes. | the present study describes the metabolic changes observed in a dietary subacute toxicity experiment with the ergot alkaloid alpha-ergocryptine in sprague-dawley rats. the observed effects on metabolic and hormonal parameters were described separately from the general toxicological effects, in view of the important role of dopamine agonists in metabolism (e.g. ergot alkaloids in fescue toxicosis). the rats were fed 0, 4, 20, 100 or 500 mg ergocryptine/kg diet for 28-32 days (equal to 0, 0.36, 1. ... | 2000 | 10908816 |
| torsional rigidity of scoliosis constructs. | a biomechanical study of the rigidity of various scoliosis constructs instrumented with and without caudal pedicle screw anchors and with none, one, or two cross-link devices. | 2000 | 10908931 |
| involvement of thyroid hormones in the effect of intracerebroventricular leptin infusion on uncoupling protein-3 expression in rat muscle. | we have shown previously that continuous (6 days) intracerebroventricular (icv) leptin infusion in normal rats resulted in decreases in food intake and body weight. a reduction of food intake imposed on control rats (pair-feeding), aimed at mimicking leptin-induced hyperphagia, produced a marked decrease in the expression of muscle uncoupling protein-3 (ucp-3), whereas icv infusion of leptin prevented such a decrease in ucp-3. to investigate an involvement of thyroid hormones in this effect of l ... | 2000 | 10909965 |
| preanesthetic train-of-four fade predicts the atracurium requirement of myasthenia gravis patients. | the most sensitive diagnostic criterion of myasthenia gravis is a decrement in the muscular response to repetitive stimulation. the authors hypothesized that myasthenia gravis patients who show a train-of-four ratio (t4/t1) < 0.9 in the preanesthetic period will have increased sensitivity to nondepolarizing neuromuscular blocking agents compared with myasthenia gravis patients with preanesthetic t4/t1 > or = 0.9. | 2000 | 10910480 |
| clinical review 115: effect of thyroxine therapy on serum lipoproteins in patients with mild thyroid failure: a quantitative review of the literature. | the objective of our study was to estimate the expected change in serum lipoprotein concentrations after treatment with t4 in patients with mild thyroid failure (i.e. subclinical hypothyroidism). our data sources included medline, between january 1966 and may 1999, and review of references from relevant articles. there were 1,786 published studies identified, 461 abstracts reviewed, 74 articles retrieved, 24 articles evaluated against predetermined entry criteria, and 13 studies systematically r ... | 2000 | 10999775 |
| effects of metyrapone administration on thyrotropin secretion in healthy subjects--a clinical research center study. | although pharmacological doses of glucocorticoids suppress tsh secretion, less is known regarding the effects of physiological variations in cortisol levels on tsh. to study this issue, 12 healthy subjects each underwent 2 studies, in random order: 1) each subject received an infusion of saline for 48 h; and 2) each subject received an infusion of saline and oral administration of metyrapone (500 mg every 4 h) for 48 h. cortisol and tsh levels were measured every 15 min during the final 24 h of ... | 2000 | 10999784 |
| thyroid function and intellectual development of infants nursed by mothers taking methimazole. | for many years, breast-feeding was forbidden if antithyroid drugs were being used. recently, limited studies have shown the relative safety of propylthiouracil and methimazole (mmi). it is not known whether mmi therapy of lactating mothers for 1 yr is safe for breast-fed infants and does not cause alterations in thyroid function and intellectual development. between 1988 and 1998, 139 thyrotoxic lactating mothers and their infants were studied. fifty-one thyrotoxic lactating mothers were treated ... | 2000 | 10999814 |
| thyroid hormone levels in cord blood of infants with acidemia at birth. | to study the funic thyroid hormone levels in cases were fetal distress during labor resulted in acidemia at birth. | 2000 | 11000504 |
| identification of essential amino acids in the bacterial alpha -mannosyltransferase acea. | the alpha-mannosyltransferase acea from acetobacter xylinum belongs to the cazy family 4 of retaining glycosyltransferases. we have identified a series of either highly conserved or invariant residues that are found in all family 4 enzymes as well as other retaining glycosyltransferases. these residues included glu-287 and glu-295, which comprise an ex(7)e motif and have been proposed to be involved in catalysis. alanine replacements of each conserved residue were constructed by site-directed mu ... | 2000 | 11001941 |
| usefulness of thyroxine and free thyroxine filter paper measurements in neonatal screening for congenital hypothyroidism of preterm babies. | low thyroxine (t4) with normal thyroid stimulating hormone (tsh) is a well known condition in preterm (pt) infants. the establishment of t4 and freet4 (ft4) values in filter paper dried blood spots in pt could provide useful information in the neonatal period. | 2000 | 11002447 |
| evaluation of new tnm lung cancer classification. | a new staging system for lung cancer was proposed by the union internationale contre le cancer in 1997, with stages i and ii subdivided and t3n0m0 assigned as stage iib. we studied the usefulness of this new classification. | 2000 | 11002579 |
| stoichiometry and inter-subunit interaction of the wedge initiation complex, gp10-gp11, of bacteriophage t4. | association of gp10 and gp11 (gp=gene product) is the first step in the assembly pathway of the wedge part of the baseplate of bacteriophage t4. the gp10-gp11 complex constitutes the six tail pins at the corners of the baseplate hexagon on the distal side. the stoichiometry of the subunits, gp10 and gp11, of this complex was determined in combination with sedimentation equilibrium, edman degradation of the complex and sodium dodecylsulfate-polyacrylamide gel electrophoresis (sds-page). from the ... | 2000 | 11004546 |
| chiropractic biophysics digitized radiographic mensuration analysis of the anteroposterior cervicothoracic view: a reliability study. | to investigate the reliability of a radiographic measurement procedure that uses a computer and sonic digitizer to determine projected spinal displacements from an ideal, normal position. | 2000 | 11004652 |
| [assessment of mediastinal tumor involvement (t factor) in lung cancer with transesophageal ultrasonography]. | transesophageal sonography was originally used to assess esophageal-gastric neoplasm. the technique may also be useful in the evaluation of pulmonary neoplasms for possible mediastinal involvement, with regard to both t and n factors. | 2000 | 11004987 |
| outcome of patients with rectal adenocarcinoma and localized pelvic non-nodal metastatic foci. | the aim of this study was to evaluate the outcome of patients with primary rectal adenocarcinoma and soft tissue metastatic foci restricted to the pelvis and to determine whether this entity, which is considered n1 disease in the american joint committee on cancer staging system, behaves like completely replaced nodal disease or the first sign of m1 disease. the clinical course for patients with this finding is not well-described in the literature. | 2000 | 11005486 |
| serum t3 and t4 concentrations in the adult rats treated with herbicide 2,4-dichlorophenoxyacetic acid. | the influence of sublethal doses of 2,4-dichlorophenoxyacetic acid (2,4-d) on serum t3 and t4 concentrations in hsd cpb: wistar rats of both sexes was studied. the trial was performed on 24 males and females respectively, each divided into three groups of 8 animals (control, groups 1 and 2). aqueous solution of the compound (11 mg/kg body weight--group 1 and 110 mg/kg body weight--group 2) or clean tap water (control group) was used. aliquots of 2.4 ml/kg body weight were administered with a sto ... | 2000 | 11005658 |
| drug therapy for hyperthyroidism in pregnancy: safety issues for mother and fetus. | hyperthyroidism (thyrotoxicosis) in pregnancy and the child bearing years is usually attributable to graves' disease. this is an autoimmune condition in which thyroid-stimulating immunoglobulins (tsi) cause hyperthyroidism. as a rule, pregnancy complicates the management of hyperthyroidism, rather than vice versa. however, patients who remain thyrotoxic during pregnancy are at increased risk of maternal and fetal complications, particularly miscarriage and stillbirth. therefore, bodyweight loss, ... | 2000 | 11005705 |
| cortical potentials associated with voluntary mandibular movements. | movement-related cortical potentials (mrcps) are negative potentials over the scalp, which gradually increase prior to voluntary movements, and might be applied to elucidate the cortical efferent function of the mandibular movements. we compared the mrcps accompanying various mandibular movements to study the motor control mechanism underlying these movements. electroencephalograms (eegs) were recorded from 11 electrodes placed over the scalp (f3, fz, f4, t3, c3, cz, c4, t4, p3, pz, and p4), acc ... | 2000 | 11005737 |
| sound velocity measurements of nuclear-ordered solid 3he along the melting curve. | we have measured the temperature dependence of the 10.98 mhz longitudinal sound velocity of solid 3he in the nuclear-ordered u2d2 phase and in the paramagnetic phase along the melting curve. the temperature dependence of the sound was attributed to the contributions from the nuclear spin system and the molar volume change along the melting curve. the sound velocity increased with temperature as t4 in the u2d2 phase and the sound anisotropy due to the exchange interaction was found to be about 10 ... | 2000 | 11005982 |
| [median sagittal mandibulotomy in head-neck tumors]. | histologically proven radical resections are the goal in patients with head and neck cancer because of improved survival rates. the frequency of histologically radical resections using the median mandibulotomy and the morbidity of this approach for tumors of the oropharynx and the parapharyngeal space are described in a patients series. | 2000 | 11006914 |
| kinetic characterization of outer-ring deiodinase activity (ord) in the liver, gill and retina of the killifish fundulus heteroclitus. | conversion of t4 to t3 is the first step in th action and deiodinases are the major determinants of th tissue availability and disposal. we here report the kinetic characterization of the outer-ring deiodinating (ord) enzymes in the liver, gill and retina of sea water-adapted killifish, by using both rt3 and t4 as substrates. in liver, by using rt3, we detected a high km (84 nm) and a low km (1.3 nm) component with kinetic characteristics similar to mammalian deiodinases di and dii. in contrast, ... | 2000 | 11007170 |
| synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase and several ligases. | the findings presented here originally arose from the suggestion that the synthesis of dinucleoside polyphosphates (np(n)n) may be a general process involving enzyme ligases catalyzing the transfer of a nucleotidyl moiety via nucleotidyl-containing intermediates, with release of pyrophosphate. within this context, the characteristics of the following enzymes are presented. firefly luciferase (ec 1.12. 13.7), an oxidoreductase with characteristics of a ligase, synthesizes a variety of (di)nucleos ... | 2000 | 11007993 |
| correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of acanthamoeba spp. | eleven acanthamoeba isolates, obtained from acanthamoeba keratitis patients, from contact lens cases of non-acanthamoeba keratitis patients, from asymptomatic individuals, from necrotic tissue, and from tap water and two reference strains were investigated by morphological, molecular biological, and physiological means in order to discriminate clinically relevant and nonrelevant isolates. all clinically relevant isolates showed acanthamoeba sp. group ii morphology. 18s ribosomal dna sequencing r ... | 2000 | 11010891 |
| expression of thyroid receptor isoforms in the human fetal central nervous system and the effects of intrauterine growth restriction. | congenital hypothyroidism is known to be associated with mental retardation which, if recognized promptly, is largely prevented by thyroid hormone replacement. intrauterine growth restriction (iugr) is a major cause of perinatal mortality and morbidity, and is also associated with neurodevelopmental delay. fetuses with iugr have reduced circulating concentrations of free thyroxine (t4) and free triiodothyronine (t3), leading to the hypothesis that a reduction in the tissue effects of thyroid hor ... | 2000 | 11012572 |
| functional connections between the suprachiasmatic nucleus and the thyroid gland as revealed by lesioning and viral tracing techniques in the rat. | frequent blood sampling via intraatrial cannula revealed daily rhythms of tsh and thyroid hormones in both male and female wistar rats. thermic ablation of the biological clock, i.e. the suprachiasmatic nucleus (scn), eliminated the diurnal peak in circulating tsh and thyroid hormones. in addition, scn lesions produced a clear decrease of 24-h mean t4 concentrations. a more pronounced effect of scn-lesions on thyroid hormones, as opposed to tsh, suggested routes of scn control additional to the ... | 2000 | 11014240 |
| growth hormone increases connexin-43 expression in the cerebral cortex and hypothalamus. | several studies indicate that systemic gh influences various brain functions. connexin-43 forms gap junctions that mediate intercellular communication and establish the astroglial syncytium. we investigated the effects of peripheral administration of bovine gh (bgh) and recombinant human insulin-like growth factor i (rhigf-i) on the expression of connexin-43 in the rat brain. hypophysectomized female sprague dawley rats were substituted with cortisol (400 microg/kg x day) and l-t4 (10 microg/kg ... | 2000 | 11014245 |
| the somatostatin analogue octreotide modulates iodothyronine deiodinase activity and pituitary neuromedin b. | somatostatin inhibits growth hormone and thyrotropin (tsh) secretion. it also enhances the inhibitory effect of thyroid hormone (th) on tsh by poorly understood mechanisms. we investigated the acute effect of the long-acting somatostatin analogue, octreotide (oct), on anterior pituitary type 1 (d1) and 2 (d2) deiodinase activity, on liver d1, and on pituitary content of neuromedin b (nb), an autocrine inhibitor of tsh secretion, which is positively regulated by thyroid hormones. euthyroid or hyp ... | 2000 | 11014308 |
| sensitive thyroid-stimulating antibody assay in whole serum containing five percent polyethylene glycol using porcine thyroid cells. | previously we reported that the amounts of cyclic adenosine monophosphate (camp) production by polyethylene glycol (peg) 22.5% precipitated fraction (pf) (crude immunoglobulin g [igg]) from thyroid-stimulating antibody (tsab)-positive serum were higher than those by peg 12.5% pf, and that peg (5%) augmented affinity purified tsab-igg-stimulated camp production in porcine thyroid cells (ptc) assay. in the present work, we studied sensitive tsab assay using whole sera in the presence of high peg c ... | 2000 | 11014309 |
| atypical hypothyroidism and the very low birthweight infant. | results of thyroid screening tests were examined retrospectively on 311,282 infants born in massachusetts from january 1, 1993 to december 31, 1996. during this period, 118 infants were found to have typical hypothyroidism, characterized by a low thyroxine (t4) and an elevated thyrotropin (tsh) on the initial newborn-screening specimen. of these, 98 were normal birthweight (nbw, > or = 2,500 g), 9 were low birthweight (lbw, 1,501-2,499 g), and 11 were very low birthweight (vlbw, < or = 1,500 g). ... | 2000 | 11014314 |
| effect of subclinical hypothyroidism on body fluid compartments. | the present study was aimed to assess the effects of subclinical hypothyroidism on body composition (bc). | 2000 | 11014384 |
| thermodynamic and structural studies of cavity formation in proteins suggest that loss of packing interactions rather than the hydrophobic effect dominates the observed energetics. | the hydrophobic effect is widely believed to be an important determinant of protein stability. however, it is difficult to obtain unambiguous experimental estimates of the contribution of the hydrophobic driving force to the overall free energy of folding. thermodynamic and structural studies of large to small substitutions in proteins are the most direct method of measuring this contribution. we have substituted the buried residue phe8 in rnase s with alanine, methionine, and norleucine. bindin ... | 2000 | 11015216 |
| impact of lead exposure on pituitary-thyroid axis in humans. | thyroid function tests (serum levels of thyroxine-t4, triiodothyronine-t3 and thyroid stimulating hormone-tsh) were performed in fifty-eight men (mean age: 31.7 +/- 10.6 years; mean duration of lead exposure: 156.9 +/- 122.7 months). these subjects were exposed to lead either as petrol pump workers or automobile mechanics. the mean whole blood lead (pb-b) levels were 2.49 +/- 0.45 micromole/l (51.90 +/- 9.40 microg/dl) in the lead exposed workers and were approximately 5 times higher than in the ... | 2000 | 11016408 |
| radical surgery for gallbladder cancer: current options. | gallbladder carcinoma is the most common malignancy of the biliary tract. there are still many controversies regarding the type of curative surgical treatment for each stage of the disease. the staging system used is the tnm classification of the international union against cancer. different patterns of spread characterize gallbladder cancer but the two main types are direct invasion and lymph node metastases; since only the depth of invasion can be easily recognized by imaging techniques, it be ... | 2000 | 11016462 |
| the success rate of identification of the sentinel lymph node in breast cancer: our training series. | a new phase of breast conserving surgery has started very recently, aimed at eliminating axillary dissection in node-negative patients by using the sentinel lymph node (sn) technique. between november 1998 and january 2000 we performed 151 operations for breast cancer on 145 patients. we performed axillary lymphoscintigraphy using 99tc-labeled human serum albumin microcolloidal particles injected subdermally in 50 patients who met our selection criteria. in this series we focused on the success ... | 2000 | 11016712 |
| cisplatin-epirubicin-paclitaxel weekly administration with g-csf support in advanced breast cancer. a southern italy cooperative oncology group (sicog) phase ii study. | it has been shown in vitro that both cisplatin and epirubicin increase the antitumor activity of paclitaxel. weekly administration could give a substantial improvement in the therapeutic index of cisplatin and paclitaxel. this study was aimed at defining the antitumor activity of a weekly cisplatin-epirubicin-paclitaxel (pet) administration in locally advanced or metastatic breast cancer patients. | 2000 | 11016746 |
| enzymatic mutation detection in the p53 gene. | the enzymatic mutation detection (emd) assay uses the bacteriophage resolvase t4 endonuclease vii, which cleaves preformed heteroduplex molecules at mismatch sites, forming two shorter fragments that can be resolved by gel electrophoresis. the method can be used to detect single and multiple base changes, as well as insertions and deletions. | 2000 | 11017933 |
| thyroid stimulating hormone levels in cord blood are not influenced by non-thyroidal mothers' diseases. | screening programs not only offer the opportunity to trace and treat almost all cases of congenital hypothyroidism but also mean large savings to the health system. however, carefully planned strategies are necessary to extend their benefits and reduce costs. | 2000 | 11018848 |
| [advanced gastric cancer: the findings of delayed phase dynamic ct and radiologic-histopathologic correlation]. | the aim of this study was to describe delayed phase dynamic ct findings of advanced (t2-t4) gastric cancer and to correlate with histopathologic findings. | 2000 | 11019578 |
| reduced global genomic repair of ultraviolet light-induced cyclobutane pyrimidine dimers in simian virus 40-transformed human cells. | the p53 tumor-suppressor gene has been implicated in the inducible activation of excision repair of ultraviolet (uv)-induced cyclobutane pyrimidine dimers (cpds) in human cells. because the large t antigen (ltag) of the simian virus 40 (sv40) binds p53 protein and can interfere with its function, it was of interest to study dna repair in normal human fibroblasts that had been transformed by sv40 compared with that in their nontransformed parental counterparts and to determine whether such transf ... | 2000 | 11020243 |
| high-dose-rate interstitial brachytherapy as a monotherapy for localized prostate cancer: treatment description and preliminary results of a phase i/ii clinical trial. | to improve results for localized prostate cancer, a prospective clinical trial of hyperfractionated iridium-192 high-dose-rate (hdr) brachytherapy as a monotherapy was initiated. | 2000 | 11020563 |
| involved-field radiotherapy alone for early-stage non-small-cell lung cancer. | to describe the outcome of involved-field radiotherapy in patients with early-stage non-small-cell lung cancer (nsclc). | 2000 | 11020567 |
| post-partum thyroiditis in south brazil presenting as thyrotoxicosis: prevalence and risk factors. | the prevalence of post-partum thyroiditis (ppt) has been reported in several countries (1.9 to 16.7%) but is not known in brazil. several factors have been associated to its development, such as a female sex of the newborn, ppt in a previous pregnancy, a family history of thyroid disease and cigarette smoking. to investigate the prevalence of ppt and its risk factors in a southern brazilian city, a three-cross-sectional observation study was performed. ppt was diagnosed in 14/284 subjects (5.3%) ... | 2000 | 11021764 |
| t4 early promoter strength probed in vivo with unribosylated and adp-ribosylated escherichia coli rna polymerase: a mutation analysis. | the consensus sequence of t4 early promoters differs in length, sequence and degree of conservation from that of escherichia coli sigma(70) promoters. the enzyme interacting with these promoters, and transcribing the t4 genome, is native host rna polymerase, which is increasingly modified by the phage-encoded adp-ribosyltransferase, alt. t4 early transcription is a very active process, possibly out-competing host transcription. the much stronger t4 promoters enhance viral transcription by a fact ... | 2000 | 11021939 |
| effect of combined treatment with calcitonin on bone densitometry of patients with treated hypothyroidism. | thyroid hormones (th) may affect bone metabolism and turnover, inducing a loss of bone mass among hyperthyroid and in hypothyroid patients under hormone replacement treatment. thyroid dysfunction leads to changes in the dynamics of parathyroid hormone (pth) and calcitonin (ct) secretion. | 2000 | 11022359 |
| eus in preoperative staging of pancreatic cancer. | endoscopic ultrasound (eus) is believed to be highly accurate in the local (t) and nodal (n) staging of pancreatic cancer. however, there are scant data concerning the predictive value of eus for resectability of pancreatic adenocarcinoma. this study was performed to determine the accuracy of tnm staging by eus in patients with pancreatic adenocarcinoma and to evaluate the role of preoperative tnm staging by eus for determining resectability in patients with pancreatic adenocarcinoma. | 2000 | 11023561 |
| exhaustive mutagenesis in silico: multicoordinate free energy calculations on proteins and peptides. | we have extended and applied a multicoordinate free energy method, chemical monte carlo/molecular dynamics (cmc/md), to calculate the relative free energies of different amino acid side-chains. cmc/md allows the calculation of the relative free energies for many chemical species from a single free energy calculation. we have previously shown its utility in host:guest chemistry (pitera and kollman, j am chem soc 1998;120:7557-7567)1 and ligand design (eriksson et al., j med chem 1999;42:868-881)2 ... | 2000 | 11025549 |
| case series: brief parent-child group therapy for childhood anxiety disorders using a manual-based cognitive-behavioral technique. | to report on a brief parent-child group therapy program for children with anxiety disorders. | 2000 | 11026186 |
| psychological functioning of recently bereaved, middle-aged women: the first 13 months. | this study examined the natural course of psychological functioning in recently bereaved middle-aged women. 69 widows were assessed four times (t1-t4) between the period of 4 to 13 mo. after the loss and were compared to a matched nonwidowed group of 57. of the scl-90 feelings of depression, agoraphobic behavior, anxiety, hostility, somatization, feelings of insufficiency, and sleep disorders were heightened at 4 mo. after bereavement compared to the norm group. significantly higher psychologica ... | 2000 | 11026420 |
| flexibility and ligand exchange in a buried cavity mutant of t4 lysozyme studied by multinuclear nmr. | the leu99-->ala mutant of t4 lysozyme contains a large internal cavity in the core of its c-terminal domain that is capable of reversibly binding small hydrophobic compounds. although the cavity is completely buried, molecules such as benzene or xenon can exchange rapidly in and out. the dynamics of the unliganded protein have been compared to the wild-type protein by measuring the nmr spin relaxation rates of backbone amide and side chain methyl nuclei. many residues surrounding the cavity were ... | 2000 | 11027141 |
| left temporal deficit of p300 in patients with schizophrenia: effects of task. | p300 is often, but not always, observed to be more reduced over left than right temporal lobes in patients with schizophrenia. the possibility that task differences contribute to the inconsistency in the literature was explored in this study. erps were collected from 17 right-handed men with schizophrenia (dsm-iiir) and 11 right-handed healthy male community controls, performing three auditory oddball tasks - respond to a target tone by: (1) counting; (2) pressing a response button with the righ ... | 2000 | 11027795 |
| hyperthyroidism in children. | this study was done to characterize the clinical features, laboratory parameters and response to therapy and outcome of childhood hyperthyroidism. the evaluation included history, examination, laboratory investigations: serum t3, t4, tsh, free t3, free t4 by ria or immunochemiluminescence (ic), antithyroid antibodies by standard techniques, bone age (ba) by greulich and pyle's method, clinical and laboratory response to treatment, and follow-up of 15 children with hyperthyroidism seen in past ei ... | 2000 | 11028118 |
| epidural analgesia-anaesthesia in obstetrics. | epidural analgesia is the most effective and innocuous technique for obstetrics. pain relief is its main indication but maternal diseases that might be decompensated by labour and delivery are also accepted indications. low doses of long-acting local anaesthetics alone or in combination with low doses of fentanyl or sufentanil provide good quality analgesia and are safe for mother and fetus. test doses in parturients lack sufficient specificity and sensitivity for detecting inadvertent intravasc ... | 2000 | 11029122 |
| ballotability of cuff to confirm the correct intratracheal position of the endotracheal tube in the intensive care unit. | the cuff ballotability method was used in 120 adult patients to confirm the correct depth of insertion of the endotracheal tube after tracheal intubation. the correct tube position was assumed when the cuff of the endotracheal tube could be felt to distend over the suprasternal notch when the pilot balloon was squeezed and the pilot balloon was felt to distend when pressure was applied over the suprasternal notch. chest radiography was performed later to confirm the position of the endotracheal ... | 2000 | 11029127 |