Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| neonatal thyroxine level and perchlorate in drinking water. | environmental contamination of drinking water has been observed for perchlorate, a chemical able to affect thyroid function. this study examines whether that exposure affected the thyroid function of newborns. neonatal blood thyroxine (t4) levels for days 1 to 4 of life were compared for newborns from the city of las vegas, nevada, which has perchlorate in its drinking water, and those from the city of reno, nevada, which does not (detection limit, 4 micrograms/l [ppb]). this study is based on b ... | 2000 | 10693082 |
| the colorado thyroid disease prevalence study. | the prevalence of abnormal thyroid function in the united states and the significance of thyroid dysfunction remain controversial. systemic effects of abnormal thyroid function have not been fully delineated, particularly in cases of mild thyroid failure. also, the relationship between traditional hypothyroid symptoms and biochemical thyroid function is unclear. | 2000 | 10695693 |
| comparison of combined spinal epidural anesthesia and epidural anesthesia for cesarean section. | epidural anesthesia (ea) is popular for cesarean section, but has some drawbacks such as incomplete block, inadequate muscle relaxation and delayed onset. combined spinal epidural anesthesia (csea) has gained increasing interest as it combines the reliability of a spinal block and the flexibility of an epidural block. we investigated the efficacy of csea that combines the main spinal and the supporting epidural anesthesia, comparing with ph-adjusted ea, for cesarean section. | 2000 | 10695917 |
| dna polymerase of the t4-related bacteriophages. | the dna polymerase of bacteriophage t4, product of phage gene 43 (gp43), has served as a model replicative dna polymerase in nucleic acids research for nearly 40 years. the base-selection (polymerase, or pol) and editing (3'-exonuclease, or exo) functions of this multifunctional protein, which have counterparts in the replicative polymerases of other organisms, are primary determinants of the high fidelity of dna synthesis in phage dna replication. t4 gp43 is considered to be a member of the "b ... | 2000 | 10697407 |
| identification of auditory evoked potentials of one's own voice. | we recorded vocalization related cortical potentials (vrcp) with complete masking of one's own voice, and separated the feedback auditory potentials following vocalization from the vrcp complex. | 2000 | 10680556 |
| crystallization and preliminary x-ray analysis of a bacteriophage t4 primase fragment. | the primase from bacteriophage t4 is a single-stranded dna-dependent rna polymerase that is one of the seven proteins that constitute the dna-replication machinery of bacteriophage t4. in an attempt to crystallize the protein, a number of variants were generated. one such construct, which includes the c-terminal region (residues 196-340), gave four different crystal forms which diffract in the 3. 5-6.0 a resolution range. | 2000 | 10666640 |
| the effect of the antithyroid drug propylthiouracil on the alternative pathway of complement in rats. | the effect of propylthiouracil (ptu) on the lytic activity of complement in rat serum was investigated in vivo. rats (180+/-10 g) were treated daily by gavage with ptu doses of 1-50 mg/200 g body weight for time intervals ranging from 1 to 30 days. serum classical pathway (cp) and alternative pathway (ap) activities were determined 24 h after the last dose. a single dose of 50 mg/200 g body weight was administered to additional groups and the animals were sacrificed after periods of 1-48 h. the ... | 2000 | 10684986 |
| posterior spinal instrumentation and fusion of a neuromuscular scoliosis in a patient with autosomal dominant osteopetrosis. | a case report of a patient with autosomal dominant osteopetrosis and neuromuscular scoliosis who required surgical instrumentation and fusion of her spine. | 2000 | 10685493 |
| strand switching during rolling circle replication of plasmid-like dna circles in the mitochondria of the higher plant chenopodium album (l.). | the structure of sigma-like mitochondrial dna molecules prepared from suspension cultured cells of chenopodium album (l.) was studied by electron microscopy. these molecules were highly variable in size, ranging from about 1 to 104 kb, and had single- and double-stranded regions typical for rolling circle replicating intermediates. partial denaturation studies confirmed that these structures constitute rolling circles. close inspection of the circle-tail junctions of the replication fork at high ... | 2000 | 10686137 |
| overexpression, crystallization and preliminary x-ray crystallographic analysis of dihydrofolate reductase from bacteriophage t4. | dihydrofolate reductase (dhfr) from bacteriophage t4 is a homodimer consisting of 193-residue subunits. it has been crystallized in the presence of the cofactor (nadph) and an inhibitor (aminopterin) at 296 k using sodium chloride as precipitant. the crystals are tetragonal, belonging to the space group p4(1)22 (or p4(3)22), with unit-cell parameters a = b = 61.14, c = 123.23 a under cryogenic conditions. the asymmetric unit contains a single subunit, with a corresponding v(m) of 2.65 a(3) da(-1 ... | 2000 | 10818362 |
| thyroidopathies. | pregnancy affects thyroid physiology in many ways: (a) the renal iodide clearance rate is increased, hence iodine requirements increase. (b) the fetal requirements for thyroid hormones and iodide are an additional problem. (c) serum thyroxine-binding globulin increases, thus producing an increase in the levels of total t4 and t3. (d) chorionic gonadotropin has a thyroid-stimulating activity. this may be compensated for by a decrease in tsh, but in some cases gestational thyrotoxicosis occurs. (e ... | 2000 | 10818394 |
| non-nucleoside hiv-1 reverse transcriptase inhibitors: synthesis and biological evaluation of novel quinoxalinylethylpyridylthioureas as potent antiviral agents. | new heterocyclic derivatives of ethylpyridylthiourea, quinoxalinylethylpyridylthiourea (qxpt) and analogues, inhibited human immunodeficiency virus type 1 (hiv-1) reverse transcriptase (rt) activity and prevented hiv-1 cytopathogenicity in t4 lymphocytes. several of these novel non-nucleoside rt inhibitors, with a substituted pyrroloquinoxalinone heteroaromatic skeleton, showed inhibitory activity against wild-type rt as well as against mutant rts containing the single amino acid substitutions l ... | 2000 | 10819438 |
| the effectiveness of preoperative intraluminal brachytherapy in preventing wall penetration and nodal involvement of rectal carcinomas. | a retrospective review was conducted of 115 patients with middle or lower rectal carcinoma, without distant metastasis, who were consecutively treated with preoperative intraluminal brachytherapy and radical surgery (ibt group). another 115 rectal carcinoma patients treated with surgery alone were reviewed as a control (nibt group). we investigated whether conventional pathologic stagings were correlated with prognosis. all tumors were diagnosed by the tnm classification. according to the distri ... | 2000 | 10819475 |
| prolactin-induced expression of intercellular adhesion molecule-1 and the accumulation of monocytes/macrophages during regression of the rat corpus luteum. | intercellular adhesion molecule-1 (icam-1) is thought to facilitate the recruitment and migration of monocytes/macrophages to sites of inflammation. here we investigated whether the luteolytic effect of prolactin in the hypophysectomized rat is associated with the expression of icam-1. in addition, we examined the effect of exogenous testosterone (or its potential conversion to estradiol endogenously) on the corpus luteum to address recent speculation that ovarian steroids might augment luteal r ... | 2000 | 10819757 |
| aqueous two-phase systems containing urea: influence of protein structure on protein partitioning. | during recombinant e. coli fermentation with high-expression levels inclusion bodies are often formed. aqueous two-phase systems have been successfully used in the presence of urea for the initial recovery step of inclusion bodies from e. coli. basic studies of the complex interactions are lacking. for a systematic study of protein partitioning in the presence of urea we selected t4-lysozyme mutants with different thermal stability as a model. the stabilization of these variants by phase compone ... | 2000 | 10820334 |
| elective ipsilateral neck irradiation of patients with locally advanced maxillary sinus carcinoma. | the current study was conducted to investigate retrospectively whether elective ipsilateral neck irradiation (eini) is effective in controlling subclinical neck disease in patients with locally advanced (t3 and t4) nonmetastatic (n0, m0) squamous cell carcinoma (scc) of the maxilla. | 2000 | 10820345 |
| thyroid hormone modulation of rat sulphotransferase mrna expression. | 1. thyroid hormones modulate sulphotransferase (sult) enzyme expression. specific substrates are not available for the study of the sult isoforms, so the regulation of hepatic sult mrna expression by thyroid hormones was examined by northern blot analysis with oligonucleotide probes specific for each sult mrna, including male-dominant phenol sult (1a1, 1c1, 1e2), female-dominant hydroxysteroid sult (20/21, 40/41, 60), and a non-sex-dependent sult, 1b1. the male and female rat were either untreat ... | 2000 | 10821164 |
| blood volume and hemoglobin after spinal cord injury. | the main purpose of this study was to investigate the difference in total blood volume and hemoglobin mass between spinal cord-injured and able-bodied individuals. | 2000 | 10821312 |
| changes in iodine metabolism during late pregnancy and lactation: a longitudinal study among sudanese women. | to elucidate the persistence, or otherwise, of the pregnancy-related changes in the iodine metabolism and thyroid function in a population residing in an area of mild iodine deficiency in the sudan. | 2000 | 10822292 |
| prognostic variables in male breast cancer. | the prognostic role of ploidy status, s phase fraction, estrogen and progesterone receptor status, and the expression of p53 and erbb-2 protein in male breast carcinoma (mbc) remains controversial. the primary objective of this study was to determine which of the common prognostic factors for female breast cancer predict prognosis in mbc. a secondary objective was to assess the impact of comorbid illnesses on survival. a retrospective review of demographic data, surgical treatment, pathological ... | 2000 | 10824754 |
| influence of biochrome on the response of metabolic hormones in pems-infected poults. | poult enteritis and mortality syndrome (pems), a disease that affects turkeys between 7 and 28 d of age, causes a severe inflammation of the intestinal tract and is characterized in poults by severe diarrhea, high morbidity, mortality, and stunting. the pems-associated mortality and growth depression is related to malabsorption and decreased metabolic activity caused, in part, by a possible insulin deficiency or insensitivity. insulin receptors are stimulated by the glucose tolerance factor (gtf ... | 2000 | 10824953 |
| anatomic considerations of costotransverse screw placement in the thoracic spine. | numerous techniques have been reported to restore spinal stability and to correct spinal deformities, including rods with wires/hooks, and rods or plates with pedicular screws. it was thought that posterior fixation of the thoracic spine through the costotransverse joint may be another alternative. | 2000 | 10825520 |
| abdominal wall recurrence after colorectal resection for cancer. | disease recurrence in the abdominal wall from a primary colorectal cancer is a poorly studied and little understood phenomenon that has received renewed attention after the recognition of port site metastases in patients after laparoscopic colorectal resections. the purpose of the present study was to define the clinical, pathologic, and management issues in patients with abdominal wall metastases from colorectal cancer. | 2000 | 10826422 |
| effect of nicotine on rat gingival fibroblasts in vitro. | tobacco smoking is considered a major risk factor for the development and progression of periodontal diseases (haber, j. and wattles, j. (1994). j. periodontol., 64, 16-23). the purpose of this study was to determine the effects of nicotine on rat gingival fibroblasts (rgf) cultured in vitro. after ether anesthesia, rat gingival tissues were obtained from the attached gingiva of a wistar rat. small fragments of gingiva were maintained in culture in petri dishes. fibroblasts developing from these ... | 2000 | 10826710 |
| radiation therapy for squamous cell carcinoma of the tonsillar region: a preferred alternative to surgery? | there are no definitive randomized studies that compare radiotherapy (rt) with surgery for tonsillar cancer. the purpose of this study was to evaluate the results of rt alone and rt combined with a planned neck dissection for carcinoma of the tonsillar area and to compare these data with the results of treatment with primary surgery. | 2000 | 10829041 |
| [the prevalence of antithyroid antibodies in lleida]. | to evaluate the prevalence of autoimmune thyroid antibody, goiter and levels of ioduria in the region of lleida. | 2000 | 10829458 |
| thyroid function and serum hepatic enzyme activity in dogs after phenobarbital administration. | phenobarbital is the drug of choice for control of canine epilepsy. phenobarbital induces hepatic enzyme activity, can be hepatotoxic, and decreases serum thyroxine (t4) concentrations in some dogs. the duration of liver enzyme induction and t4 concentration decreases after discontinuation of phenobarbital is unknown. the purpose of this study was to characterize the changes in serum total t4 (tt4), free t4 (ft4), thyroid-stimulating hormone (tsh), cholesterol and albumin concentrations, and act ... | 2000 | 10830541 |
| low-dose bupivacaine-fentanyl spinal anesthesia for cesarean delivery. | the hypotension following spinal anesthesia remains commonplace in cesarean delivery. intrathecal opioids are synergistic with local anesthetics and intensify sensory block without increasing sympathetic block. the combination makes it possible to achieve spinal anesthesia with otherwise inadequate doses of local anesthetic. we hypothesized that this phenomenon could be used to provide spinal anesthesia for cesarean delivery while incurring less frequent hypotension. | 2000 | 10834776 |
| determining epidural catheter location using nerve stimulation with radiological confirmation. | the use of epidural stimulation to confirm epidural catheter placement has been shown. this case report describes the benefits and problems of using the epidural stimulation test to confirm epidural catheter placement and provides supporting evidence for these observations using radiological imaging. | 2000 | 10834789 |
| use of a non-rigid region in t4 lysozyme to design an adaptable metal-binding site. | it is not easy to find candidate sites within a given protein where the geometry of the polypeptide chain matches that of metal-binding sites in known protein structures. by choosing a location in t4 lysozyme that is inherently flexible, it was possible to engineer a two-histidine site that binds different divalent cations. crystallographic analysis shows that the geometry of binding of zinc is distorted tetrahedral while that of cobalt and nickel is octahedral. insofar as spectroscopic data can ... | 2000 | 10835104 |
| the production of hemicellulases by aerobic fungi on medium containing residues of banana plant as substrate. | trichoderma harzianum strains t4 and t6, acrophialophora nainiana, and humicola grisea var. thermoidea were screened for their ability to produce carbohydrate-degrading enzyme activities in a medium containing banana plant residue as the carbon source. the best balance of enzyme activities was obtained from cultures of h. grisea var. thermoidea. xylanase activity from crude extract of a. nainiana had a maximum activity at ph 5.5-7.0 and a temperature range of 50-55 degrees c. it was stable up to ... | 2000 | 10835259 |
| one protein from two open reading frames: mechanism of a 50 nt translational bypass. | translating ribosomes bypass a 50 nt coding gap in order to fuse the information found in the two open reading frames (orfs) of bacteriophage t4 gene 60. this study investigates the underlying mechanism by focusing on the competition between initiation of bypassing and termination at the end of the first orf. while nearly all ribosomes initiate bypassing, no more than 50% resume translation in the second orf. two previously described cis-acting stimulatory signals are critical for favoring initi ... | 2000 | 10835364 |
| preoperative radiation with concurrent 5-fluorouracil for locally advanced t4-primary rectal cancer. | in ct4-rectal carcinoma disease-free margins often cannot be obtained by primary surgery, and even if total en bloc resection is accomplished, local failure remains high with surgery alone. herein we report on the curative resectability rate, acute toxicities, surgical complications, local control and 5-year survival rates achieved with a more aggressive multimodality regimen, including preoperative radiochemotherapy. | 2000 | 10812388 |
| thyroid hormone metabolism and action in the brain and pituitary. | brain and pituitary development, maturation, and function critically depend on thyroid hormone availability. the identification of several forms of nuclear t3-receptors, the region-specific expression of deiodinase isoenzymes in brain and pituitary, and the molecular analysis of thyroid hormone-responsive genes in fetal, newborn, and adult brain opened a new era in the understanding of thyroid hormone action. these integrated networks of receptors, deiodinases, and thyroid hormone responsive gen ... | 2000 | 10812455 |
| [the influence of hormones of stress-promoting system on the course of acute ischemic stroke]. | experimental and clinical studies have proved an important role of the reaction of a stress-realizing endocrine system for both a course and an outcome of acute somatic diseases. that is why plasma concentrations of thyroxine (t4), triiodo-thyronine (t3), thyrotropic (tth) and adrenocorticotropic (acth) hormones as well as of renin were measured during the 1-st week of acute ischemic stroke (ais) in relevant patients for determination of their prognostic significance. 16 patients with hemispheri ... | 2000 | 10812666 |
| the 'mysterious' disease in swedish moose. concentrations of trace elements in liver and kidneys and clinical chemistry. comparison with experimental molybdenosis and copper deficiency in the goat. | moose (alces alces l.) affected by a disease with unknown aetiology were compared with healthy moose regarding trace element concentrations in liver and kidneys, as well as certain clinical chemical parameters of blood plasma. the diseased moose showed decreased hepatic concentrations of cu, cd and mg. renal concentrations of cd, co, mg and mn were also decreased. substantially increased concentrations in both liver and kidneys were seen for al, ca, fe, pb and zn, while se and mo were increased ... | 2000 | 10813451 |
| experimental copper and chromium deficiency and additional molybdenum supplementation in goats. ii. concentrations of trace and minor elements in liver, kidneys and ribs: haematology and clinical chemistry. | since the mid-1980s a previously undescribed disease has affected moose in south-western sweden. investigations made to reveal evidence of a viral aetiology have proved unsuccessful. trace element studies in apparently healthy moose shot during regular hunting suggested a trace element imbalance, with excessive molybdenum uptake causing secondary copper deficiency. the results also indicated a possible chromium deficiency. to verify this hypothesis, an experimental study was performed in male go ... | 2000 | 10813454 |
| cranial irradiation of female rats causes dose-dependent and age-dependent activation or inhibition of pubertal development. | cranial irradiation in prepubertal children with leukemia or brain tumors can lead to precocious or in high doses to late puberty. to unravel the underlying mechanisms, we developed a rat model with selective cranial co60-irradiation technique. infantile (12-16 d old) or juvenile (21-23 d old) female sprague-dawley rats received a single dose of 4, 5, 6, 9 or 2 x 9 gy (at days 21 and 23). each group consisted of 7-20 animals. high radiation doses (9 gy and more) caused retardation of sexual deve ... | 2000 | 10813581 |
| combined modality treatment of locally advanced breast carcinoma in elderly patients or patients with severe comorbid conditions using tamoxifen as the primary therapy. | the purpose of the current study was to evaluate the objective response rate and possibility of breast-conserving surgery using neoadjuvant tamoxifen in the multimodality treatment, including surgery and radiotherapy, of elderly or frail patients with locally advanced breast carcinoma. | 2000 | 10813717 |
| a novel neurotensin peptide analog given extracranially decreases food intake and weight in rodents. | neurotensin decreases food intake in the rat when injected into the cerebral ventricles. we tested the effect of a novel neurotensin analog (nt69l), injected intra-peritoneally (i.p.), on weight gain and food intake in rats. sprague-dawley rats (270 g) were injected i. p. with either saline or nt69l at 0.001 or 0.010 mg/kg. in further experiments, larger rats at a more steady state on the growth curve (400 g) were injected with either saline or 0.010 or 1 mg/kg nt69l. food intake, water consumpt ... | 2000 | 10814731 |
| autoimmune thyroid disease and antiphospholipid antibodies. | autoimmune thyroid disease (atd) is associated with circulating autoantibodies reactive with epitopes on thyroid tissue and that are thought to be pathogenic in the development of these diseases. antiphospholipid antibodies (apla) are a family of immunoglobulins that recognize a variety of plasma proteins in association with anionic phospholipids. these antibodies may lead to a number of clinical syndromes including venous and arterial thromboses, thrombocytopaenia, and recurrent fetal loss. we ... | 2000 | 10815793 |
| specificity of damage recognition and catalysis of dna repair. | a common feature of dna repair enzymes is their ability to recognize the damage independently of sequence in which they are found. the presence of a flipped out base inserted into the protein in several dna-enzyme complexes suggests a contribution to enzyme specificity. molecular simulations of damaged dna indicate that the damage produces changes in dna structure and changes the dynamics of dna bending. the reduced bending force constant can be used by the enzyme to induce dna bending and facil ... | 2000 | 10816003 |
| hypothyroid myopathy-pathological and ultrastructural study. | seventeen patients with hypothyroid myopathy were studied before and after thyroxine (t4) treatment. the severity of clinical myopathy was assessed with the aid of a modified rating scale. laboratory findings including thyroid function, serum creatine kinase (ck), and electromyography were assessed at regular time intervals until a final muscle biopsy was performed. the average period of follow-up was 1.8 years. the authors emphasize: 1) in skeletal muscle pathology of hypothyroidism, the fiber ... | 2000 | 10816989 |
| structure-effect relationships of amiodarone analogues on the inhibition of thyroxine deiodination. | amiodarone (ami) has proven to be a potent anti-arrhythmic compound. due to the structural similarity between ami and thyroid hormone, it is possible that the drug could inhibit the activity of the 5'-thyroxine-deiodinase. | 2000 | 10805058 |
| the flavin coenzymes: a new class of group i intron inhibitors. | effects of the coenzyme flavin mononucleotide (fmn) and its analogs on the self-splicing of primary transcripts of the phage t4 thymidylate synthase gene (td) have been investigated. among all flavins and analogs tested, the lumichrome was the most inhibitory. the kinetic analysis demonstrated that fmn acts as a competitive inhibitor for the td intron rna with a ki of 1.86 mm although it does not possess a guanidino group in its structure. fmn is able to inhibit the first step of the self-splici ... | 2000 | 10806339 |
| hormone profiles in humans experiencing military survival training. | clinical models of the human response to intense, acute stress have been limited to laboratory settings or cross sectional characterizations. as a result, data about the sensitivity of the human neuroendocrine activation to realistic stressors of varying magnitudes are limited. the u.s. army survival course offers a unique opportunity to examine, in a controlled manner, the human response to acute, realistic, military stress. | 2000 | 10807962 |
| quality of life and disease-specific functional status following microvascular reconstruction for advanced (t3 and t4) oropharyngeal cancers. | in an effort to evaluate quality-of-life benefits of ablative head and neck cancer surgery and microvascular reconstruction, a longitudinal study was undertaken in which patients with t3 or t4 oropharyngeal cancers without systemic metastases at presentation were administered both general and disease-specific quality-of-life instruments preoperatively and postoperatively. in an initial prospective pilot study, 17 cancer patients were evaluated both preoperatively and postoperatively using the me ... | 2000 | 10809090 |
| oral cancer in jordan: a retrospective study of 118 patients. | to evaluate clinical features, diagnosis, and treatment of oral cancer in jordan. | 2000 | 10810170 |
| the nucleotide sequence and genome organization of japanese iris necrotic ring virus, a new species in the genus carmovirus. | the genome of japanese iris necrotic ring virus (jinrv) consists of a positive-sense ssrna of 4014 nucleotides with six major open reading frames (orfs). a 5'-non-coding region of 31 nucleotides precedes the first initiation codon. like carnation mottle virus (carmv), the 5'-proximal three orfs encode a 26 kda protein (p26) and two readthrough proteins, i.e. an 85 kda putative rna replicase (p85) and a 99 kda protein (p99). the central orf encodes a small 8 kda protein (p8). the 3'-proximal orf ... | 2000 | 10795530 |
| cyclin d1 expression is useful as a prognostic indicator for advanced esophageal carcinomas, but not for superficial tumors. | the purpose of the present study was to define the overexpression of cyclin d1 in superficial and advanced esophageal carcinomas and to investigate whether the expression of this molecule indicates a poor prognosis. this study included 41 patients with superficial esophageal carcinomas (tis and t1) and 48 patients with advanced esophageal carcinomas (t2, t3, and t4). the expression of cyclin d1 in surgically resected specimens was evaluated immunohistochemically with a monoclonal antibody. posit ... | 2000 | 10795746 |
| systemic circulation and cerebral oxygenation during head-up tilt in spinal cord injured individuals. | to compare tilt-induced alterations in cardiovascular homeostasis and cerebral oxygenation of spinal cord-injured (sci) to able-bodied (ab) individuals. | 2000 | 10795936 |
| interactions of proteins in aqueous electrolyte solutions from fluorescence anisotropy and circular-dichroism measurements. | understanding aqueous protein-protein interactions is crucial for the development of a molecular-thermodynamic model for salt-induced protein precipitation. in addition, protein interactions are important in many disease states, including cataract formation and alpha-amyloid diseases. fluorescence anisotropy provides a means to measure intermolecular interactions. in this work, monomer-dimer equilibrium of the peptide t4 lys(11-36) was studied by fluorescence anisotropy over the ph range 4-7 and ... | 2000 | 10796032 |
| intracellular ph regulation in a nonmalignant and a derived malignant human breast cell line. | tumor cells in vivo often exist in an ischemic microenvironment that would compromise the growth of normal cells. to minimize intracellular acidification under these conditions, these cells are thought to upregulate h(+) transport mechanisms and/or slow the rate at which metabolic processes generate intracellular protons. proton extrusion has been compared under identical conditions in two closely related human breast cell lines: nonmalignant but immortalized hmt-3522/s1 and malignant hmt-3522/t ... | 2000 | 10797312 |
| high- and low-dose interferon alfa-2b in high-risk melanoma: first analysis of intergroup trial e1690/s9111/c9190. | pivotal trial e1684 of adjuvant high-dose interferon alfa-2b (ifnalpha2b) therapy in high-risk melanoma patients demonstrated a significant relapse-free and overall survival (rfs and os) benefit compared with observation (obs). | 2000 | 10856105 |
| 'needlescopic' video-assisted thoracic surgery for palmar hyperhidrosis. | the video-assisted thoracic surgery (vats) approach for thoracodorsal sympathectomy has been well accepted. we report the use of ultra-fine thoracoscopic equipment for this procedure, based on the experience from two centers in asia. | 2000 | 10856862 |
| induction chemotherapy followed by concurrent chemotherapy and high-dose radiotherapy for locally advanced squamous cell carcinoma of the upper-thoracic and midthoracic esophagus. | the purpose of this study was to evaluate the efficacy and toxicity of an induction chemotherapy schedule followed by high-dose radiotherapy and concurrent chemotherapy for locally advanced squamous cell carcinomas of the upper and midthoracic esophagus. patients were treated with three courses of fluorouracil, leucovorin, etoposide, and cisplatin-containing induction chemotherapy followed by high-dose external beam radiotherapy to 65 gy in 6 weeks for t4 and obstructing t3 tumors. transversable ... | 2000 | 10857883 |
| dna bending is a determinant of calicheamicin target recognition. | calicheamicin is a hydrophobic enediyne antibiotic that binds noncovalently to dna and causes sequence-selective oxidation of deoxyribose. while the drug makes several base contacts along the minor groove, the diversity of binding-site sequences and the effects of dna conformation on calicheamicin-induced dna cleavage suggest that sequence recognition per se is not the primary determinant of target selection. we now present evidence that calicheamicin bends its dna targets. using a gel mobility ... | 2000 | 10858311 |
| [prognostic factors in the treatment of advanced cervical cancer. a retrospective study]. | the value of prognostic factors in patients with advanced cervix carcinoma treated by radiotherapy was assessed in a retrospective study. | 2000 | 10858729 |
| the solution structure and internal motions of a fragment of the cytidine-rich strand of the human telomere. | we present the solution structure of d(cccta2cccta2cccta2ccct), a fragment of the vertebrate telomere which folds intramolecularly. the four cytidine stretches form an i-motif which includes six intercalated c.c+ pairs and terminates with the cytidines at the 5' extremity of each stretch. above, the second ta2 linker loops across one of the narrow grooves, while at the bottom, the first and third linkers loop across the wide grooves. at 30 degrees c, the spectra of the first and third linkers ar ... | 2000 | 10860727 |
| crystal structure of a pol alpha family dna polymerase from the hyperthermophilic archaeon thermococcus sp. 9 degrees n-7. | the 2.25 a resolution crystal structure of a pol alpha family (family b) dna polymerase from the hyperthermophilic marine archaeon thermococcus sp. 9 degrees n-7 (9 degrees n-7 pol) provides new insight into the mechanism of pol alpha family polymerases that include essentially all of the eukaryotic replicative and viral dna polymerases. the structure is folded into nh(2)- terminal, editing 3'-5' exonuclease, and polymerase domains that are topologically similar to the two other known pol alpha ... | 2000 | 10860752 |
| molecular architecture of bacteriophage t4 capsid: vertex structure and bimodal binding of the stabilizing accessory protein, soc. | t4 encodes two dispensable proteins that bind to the outer surface of the mature capsid. soc (9 kda) stabilizes the capsid against extremes of alkaline ph and temperature, but hoc (40 kda) has no perceptible effect. both proteins have been developed as display platforms. their positions on the hexagonal surface lattice of gp23*, the major capsid protein, were previously defined by two-dimensional image averaging of negatively stained electron micrographs of elongated variant capsids. we have ext ... | 2000 | 10860886 |
| characterization of bacteriophage t4-coordinated leading- and lagging-strand synthesis on a minicircle substrate. | the dna replication complex of bacteriophage t4 has been assembled as a single unit on a minicircle substrate with a replication fork that permits an independent measurement of the amount of dna synthesis on both the leading and lagging strands. the assembled replisome consists of the t4 polymerase [gene product 43 (gp43)], clamp protein (gp45), clamp loader (gp44/62), helicase (gp41), helicase accessory factor (gp59), primase (gp61), and single-stranded dna binding protein (gp32). we demonstrat ... | 2000 | 10860983 |
| risk factors for congenital hypothyroidism: an investigation of infant's birth weight, ethnicity, and gender in california, 1990-1998. | approximately 85% of primary congenital hypothyroidism (ch) is sporadic and due to malformations of the thyroid gland. past studies have reported an increased birth weight among infants with ch. we have attempted to replicate and expand these observations, examining the association between different birth weight categories and ch stratified by infant's sex. we have also examined the prevalence of ch by mother's age and infant's ethnicity, gender, and year of birth. | 2000 | 10861631 |
| the duplication of an eight-residue helical stretch in staphylococcal nuclease is not helical: a model for evolutionary change. | a common method of evolutionary change is gene duplication, followed by other events that lead to new function, decoration of folds, oligomerization, or other changes. as part of a study on the potential for evolutionary change created by duplicated sequences, we have carried out a crystallographic study on a mutant of staphylococcal nuclease in which residues 55-62 have been duplicated in a wild-type variant termed phs. in the parental protein (phs) these residues form the first two turns of a ... | 2000 | 10861938 |
| preoperative evaluation of colorectal neoplasms by colonoscopic miniprobe ultrasonography. | to investigate the value of colonoscopic miniprobe ultrasonography for preoperative staging of colorectal neoplasms. | 2000 | 10862194 |
| the effect of nodal status on determinants of initial treatment response and patterns of relapse-free survival in nasopharyngeal carcinoma. | to study the effect of regional nodal status on predictors of treatment response, failure patterns, and the time-dependent nature of the various pattern of relapse via a hazard function analysis. | 2000 | 10863054 |
| experience with total skin electron beam therapy in combination with extracorporeal photopheresis in the management of patients with erythrodermic (t4) mycosis fungoides. | we compared the prognosis of patients with erythrodermic mycosis fungoides (mf) administered total skin electron beam radiation (tseb) plus neoadjuvant, concurrent, and adjuvant extracorporeal photopheresis (ecp) with the prognosis of patients administered only tseb. outcomes of clinical interest include disease-free survival (dfs), progression-free survival (pfs), overall survival (os), and cause-specific survival (css). | 2000 | 10863224 |
| squamous cell carcinoma of the oral tongue: an analysis of prognostic factors. | to identify the prognostic significance of different factors in patients with squamous cell carcinoma of the tongue. | 2000 | 10864725 |
| effects of temperature strategy during cardiopulmonary bypass on cerebral oxygen balance. | cerebral injury is the most serious complication of cardiopulmonary perfusion (cpb). with the advent of warm heart surgery, the effect of temperature strategy during perfusion and its effect on cerebral oxygen balance needs further study. | 2000 | 10836213 |
| factors determining outcome after surgical resection of t3 and t4 lung cancers of the superior sulcus. | the treatment of superior sulcus lung cancers is evolving and preoperative chemotherapy is increasingly used. to establish a historical benchmark against which new therapies can be assessed, we reviewed our 24-year experience with patients undergoing thoracotomy for lung cancers of the superior sulcus. | 2000 | 10838531 |
| contribution of de novo protein synthesis to the hypertrophic effect of igf-1 but not of thyroid hormones in adult ventricular cardiomyocytes. | enhanced expression of igf-1 occurs in left ventricular hypertrophy (lvh) associated with systemic hypertension. cardiac dysfunction accompanied by lvh is also observed in hyperthyroidism. | 2000 | 10839201 |
| sentinel lymph node biopsy for melanoma: experience with 234 consecutive procedures. | sentinel lymph node biopsy is increasingly used to identify occult metastases in regional lymph nodes of patients with melanoma. selection of patients for sentinel lymph node biopsy and subsequent lymphadenectomy is an area of debate. the purpose of this study was to describe a large clinical series of these biopsies for cutaneous melanoma and to identify patients most likely to gain useful clinical information from sentinel lymph node biopsy. the indiana university melanoma program computerized ... | 2000 | 10839392 |
| structural heterogeneity in intramolecular dna triple helices. | oligodeoxynucleotides designed to form intramolecular triple helices are widely used as model systems in thermodynamic and structural studies. we now report results from uv, raman and nmr experiments demonstrating that the strand polarity, which also determines the orientation of the connecting loops, has a considerable impact on the formation and stability of pyr x pur x pyr triple helices. there are two types of monomolecular triplexes that can be defined by the location of their purine tract ... | 2000 | 10839455 |
| scrotal dog bites. | dog bites to the scrotum are rare but they potentially result in morbidity if improperly managed. | 2000 | 10840424 |
| nerve-sparing surgery in rectal cancer: feasibility and functional results. | traditional rectal cancer surgery has been burdened with a high rate of sexual and urinary dysfunctions due to intraoperative injury or the cutting of the sympathetic and/or parasympathetic nerves. the experience acquired in the last ten years with total mesorectal excisions has permitted the use of the "nerve-sparing" technique. the present study regards 239 patients from two surgical centres, most of whom underwent sphincter-saving radical surgery between 1994 and 1998 with the above mentioned ... | 2000 | 10840933 |
| evidence for unfolding of the single-stranded gcca 3'-end of a trna on its aminoacyl-trna synthetase from a stacked helical to a foldback conformation. | the conformation of a trna in its initial contact with its cognate aminoacyl-trna synthetase was investigated with the escherichia coli glutamyl-trna synthetase-trna(glu) complex. covalent complexes between the periodate-oxidized trna(glu) and its synthetase were obtained. these complexes are specific since none were formed with any other oxidized e. coli trna. the three major residues cross-linked to the 3'-terminal adenosine of oxidized trna(glu) are lys115, arg209, and arg48. modeling of the ... | 2000 | 10841758 |
| [therapy of hypothyroidism. t4 or better t3/t4?]. | 2000 | 10842475 | |
| changes in plasma low-density lipoprotein (ldl)- and high-density lipoprotein cholesterol in hypo- and hyperthyroid patients are related to changes in free thyroxine, not to polymorphisms in ldl receptor or cholesterol ester transfer protein genes. | thyroid function disorders lead to changes in lipoprotein metabolism. both plasma low-density lipoprotein cholesterol (ldl-c) and high-density lipoprotein cholesterol (hdl-c) increase in hypothyroidism and decrease in hyperthyroidism. changes in ldl-c relate to altered clearance of ldl particles caused by changes in expression of ldl receptors on liver cell surfaces. changes in cholesterol ester transfer activity partly explain changes in hdl-c. it has been suggested that the magnitude of these ... | 2000 | 10843165 |
| pria and phage t4 gp59: factors that promote dna replication on forked dna substrates microreview. | the initiation of dna synthesis on forked dna templates is a vital process in the replication and maintenance of cellular chromosomes. two proteins that promote replisome assembly on dna forks have so far been identified. in phage t4 development the gene 59 protein (gp59) assembles replisomes at d-loops, the sites of homologous strand exchange. bacterial pria protein plays an analogous function, most probably restarting replication after replication fork arrest with the aid of homologous recombi ... | 2000 | 10844643 |
| antisense rna-dependent transcription termination sites that modulate lysogenic development of satellite phage p4. | in the lysogenic state, bacteriophage p4 prevents the expression of its own replication genes, which are encoded in the left operon, through premature transcription termination. the phage factor responsible for efficient termination is a small, untranslated rna (ci rna), which acts as an antisense rna and controls transcription termination by pairing with two complementary sequences (seqa and seqc) located within the leader region of the left operon. a rho-dependent termination site, timm, was p ... | 2000 | 10844696 |
| quantitative assessment of pituitary resistance to thyroid hormone from plots of the logarithm of thyrotropin versus serum free thyroxine index. | previous studies have shown that, in patients with primary alterations in thyroid hormone secretion, the level of the natural logarithm of serum tsh (lntsh) is negatively related to the level of free t4. because such patients can generally be assumed to exhibit normal tissue responsivity to thyroid hormone, we were interested in determining whether the lntsh/free t4 index (fti) relationship in patients with established thyroid hormone resistance (thr) exhibit a lower slope than patients with nor ... | 2000 | 10852467 |
| phage t4-like intermediates of dna replication and recombination in the mitochondria of the higher plant chenopodium album (l.). | we have studied intermediates of the recombination and replication of chromosomal mitochondrial (mt) dna prepared from suspension cultured cells of chenopodium album (l.) by electron microscopy during the whole growth cycle. we identified several types of potential recombination and replication intermediates including rosette-like structures, as well as other branched and sigma-like molecules. the absolute and relative amounts of these structures changed dramatically during the growth cycle, ind ... | 2000 | 10853767 |
| induction of oocyte maturation by jun-n-terminal kinase (jnk) on the oncogenic ras-p21 pathway is dependent on the raf-mek signal transduction pathway. | we have previously found that microinjection of activated mek (mitogen activated kinase kinase) and erk (mitogen-activated protein; map kinase) fails to induce oocyte maturation, but that maturation, induced by oncogenic ras-p21 and insulin-activated cell ras-p21, is blocked by peptides from the ras-binding domain of raf. we also found that jun kinase (jnk), on the stress-activated protein (sap) pathway, which is critical to the oncogenic ras-p21 signal transduction pathway, is a strong inducer ... | 2000 | 10854130 |
| [direct effect of anesthetic inducers on the electromyographic signals of the adductor pollicis]. | to analyze the effect of thiopental, propofol, etomidate and midazolam on electromyographic study of the adductor pollicis before muscle relaxation. | 2000 | 10846912 |
| the combined pituitary function test in children: an evaluation of the clinical usefulness of trh and lhrh stimulation tests through a retrospective analysis of one hundred and twenty six cases. | the combined pituitary function test is routinely used in the endocrine investigation of short children. the trh and luteinising hormone-releasing hormone (lhrh) response tests have been shown to be of minimal value in adults. we have evaluated the clinical utility of these tests in the context of combined pituitary function testing in children. | 2000 | 10848877 |
| energy metabolism and thyroid hormone levels of growing rats in response to different dietary proteins--soy protein or casein. | energy balances were measured by indirect calorimetry in four experiments on male growing rats, fed restrictively on isoenergetic and isonitrogenous (10% cp) diets based on either casein supplemented with methionine, or soy protein isolate (experiments 1, 2 and 3) and soy protein isolate supplemented with methionine (experiment 0), respectively. at the end of experiments the rats were killed for body analysis and determination of thyroid hormones and lipids in blood as well as mitochondrial resp ... | 2000 | 10849866 |
| c-abl expression in oral squamous cell carcinomas. | c-abl is proto-oncogene product. c-abl has roles in signal transduction, cell cycle regulation, and inhibition of apoptosis. there are many reports about c-abl function in hematopoietic cells, but few are concerned with solid tumors. in the present study, biopsy specimens from 44 patients with oral squamous cell carcinomas were subjected to immunohistochemistry, and the expression levels of c-abl were correlated with clinicopathological features. statistical analyses revealed that c-abl expressi ... | 2000 | 10889926 |
| partial hepatectomy of marmoset: clinical and pathological effects and utility in microsomal enzyme analysis. | liver biopsy based on a partial hepatectomy technique (shearing) was performed in 10 common marmosets (callithrix jacchus). this is a preliminary study to evaluate the effects of drugs on hepatic microsomal enzymes: cytochrome p-450 and t4 uridine diphosphate glucuronyl transferase (t4-udpgt), by comparing post-treatment values with pre-treatment values individually with a limited number of animals. the effects of the biopsy on clinical findings and liver pathology were evaluated during the firs ... | 2000 | 10889947 |
| generation and structural analysis of soluble oligomeric gp140 envelope proteins derived from neutralization-resistant and neutralization-susceptible primary hiv type 1 isolates. | we generated dna constructs expressing soluble truncated forms of the envelope of sf162, a neutralization-resistant primary human immunodeficiency virus type 1 isolate, and sf162av2, a neutralization-susceptible virus derived from sf162 after the deletion of 30 amino acids from the v2 loop. the constructs express the entire gp120 subunit and the extracellular region of the gp41 subunit, with either the presence ("cleaved" forms, designated gp140c) or the absence ("fused" forms, designated gp140f ... | 2000 | 10890360 |
| influence of dopexamine on hemodynamics, intramucosal ph, and regulators of the macrocirculation and microcirculation in patients undergoing abdominal aortic surgery. | to investigate whether dopexamine hydrochloride beneficially influences splanchnic perfusion and regulators of the macrocirculation and microcirculation in patients undergoing surgery of the abdominal aorta. | 2000 | 10890482 |
| a hypothesis for dna viruses as the origin of eukaryotic replication proteins. | the eukaryotic replicative dna polymerases are similar to those of large dna viruses of eukaryotic and bacterial t4 phages but not to those of eubacteria. we develop and examine the hypothesis that dna virus replication proteins gave rise to those of eukaryotes during evolution. we chose the dna polymerase from phycodnavirus (which infects microalgae) as the basis of this analysis, as it represents a virus of a primitive eukaryote. we show that it has significant similarity with replicative dna ... | 2000 | 10888648 |
| the influence of the radicality of resection and dose of postoperative radiation therapy on local control and survival in carcinomas of the upper aerodigestive tract. | to evaluate dose concepts in postoperative irradiation of carcinomas of the upper aerodigestive tract according to the radicality of resection. | 2000 | 10889383 |
| brachytherapy for primary and recurrent nasopharyngeal carcinoma: 20 years' experience at long beach memorial. | we evaluated treatment outcomes of patients with mostly locally advanced primary and recurrent cancer of the nasopharynx managed with interstitial and intraluminal brachytherapy. | 2000 | 10889385 |
| hmg1 protein stimulates dna end joining by promoting association of dna molecules via their ends. | high mobility group (hmg) 1 protein is a highly abundant and an evolutionarily conserved chromosomal protein with two homologous dna-binding domains (hmg boxes), a and b, attached by a short basic region to an acidic c-terminal tail. the protein has been implicated in a number of fundamental biological processes including dna replication, transcription, recombination and repair. we demonstrate that hmg1 is able to enhance cohesive-end and blunt-end dna ligation by t4 dna ligase via its b domain. ... | 2000 | 10866811 |
| [treatment of epidermoid carcinoma of the oral cavity and oropharynx: our result (1991-1995)]. | in a study of 79 patients diagnosed as squamous cell carcinoma of the oropharynx and oral cavity, we compared the results obtained with two different types of initial treatment (surgery versus irradiation). patients were grouped by tumor size: small tumors (t1 and t2) and bigger tumors (t3 and t4). thirty-one patients had t1-t2 tumors:17 treated surgically with an 83% cure rate, 14 treated by irradiation with a 71% cure rate. forty-three patients had t3-t4 tumors: 38 treated by irradiation with ... | 2000 | 10867395 |
| type ii and type iii monodeiodinase activities in the skin of untreated and propylthiouracil-treated cashmere goats. | the presence or absence of types i, ii and iii iodothyronine monodeiodinase enzymes (mdi, mdii and mdiii) and their levels of activity in the skin of goats, which were orally dosed for 60 days with 0, 1.1, 2.2, 4.4, 8.8, 17.5, or 35 mg(-1)kg liveweight day(-1)of the anti-thyroid, enzyme-inhibiting drug, propylthiouracil (ptu), were determined. contrary to our earlier report that ptu did not influence skin mdii activity, the currect more thorough investigation (in terms of numbers of observations ... | 2000 | 10756128 |
| breast cancer progression and expression of blood group-related tumor-associated antigens. | there is sufficient evidence that blood group related lewis antigens are tumor-associated molecules. we have conducted immunohistochemical analysis of the expression of lewis antigens in breast cancer tissue as an indicator of the degree of malignancy and as a prognostic factor. the studies were performed by examining 43 female patients diagnosed with invasive ductal carcinoma of the breast. postoperative specimens were stained immunohistochemically using a panel of monoclonal antibodies (mabs) ... | 2000 | 10868792 |
| receptor-mediated endocytosis of transthyretin by ependymoma cells. | transthyretin (ttr) is involved in the transport of thyroxine (t4) and retinol-binding protein (rbp) in cerebrospinal fluid (csf) and serum. ttr is secreted in the csf by the epithelial cells of choroid plexus. the binding of [(125)i]ttr to cultured ependymoma cells which form the brain cerebrospinal barrier, was studied to determine whether these cells carry receptor(s) for ttr. ttr was bound by ependymoma cells in a time-dependent manner reaching equilibrium within 2 h. scatchard analysis was ... | 2000 | 10869517 |
| goiter and impairment of thyroid function in acromegalic patients: basal evaluation and follow-up. | we evaluated morphological, biochemical and cytological thyroid parameters in acromegalic patients, investigated before and after treatment for acromegaly. | 2000 | 10871160 |
| interaction of the bacteriophage t4 gene 59 helicase loading protein and gene 41 helicase with each other and with fork, flap, and cruciform dna. | bacteriophage t4 gene 59 helicase loading protein accelerates the loading of t4 gene 41 dna helicase and is required for recombination-dependent dna replication late in t4 phage infection. the crystal structure of 59 protein revealed a two-domain alpha-helical protein, whose n-terminal domain has strong structural similarity to the dna binding domain of high mobility group family proteins (mueser, t. c., jones, c. e., nossal, n. g., and hyde, c. c. (2000) j. mol. biol. 296, 597-612). we have pre ... | 2000 | 10871615 |