Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs. | the extensive genetic diversity of porcine reproductive and respiratory syndrome virus (prrsv) strains is a major obstacle for vaccine development. we previously demonstrated that chimeric prrsvs in which a single envelope gene (orf3, orf4, orf5 or orf6) was shuffled via dna shuffling had an improved heterologous cross-neutralizing ability. in this study, we incorporate all of the individually-shuffled envelope genes together in different combinations into an infectious clone backbone of prrsv m ... | 2015 | 26342466 |
| persistence and retention of porcine reproductive and respiratory syndrome virus in stable flies (diptera: muscidae). | we investigated the acquisition of porcine reproductive and respiratory syndrome (prrs) virus by the stable fly (diptera: muscidae; stomoxys calcitrans (l.)) through a bloodmeal, and virus persistence in the digestive organs of the fly using virus isolation and quantitative reverse-transcription pcr (qrt-pcr). stable flies were fed blood containing live virus, modified live vaccine virus, chemically inactivated virus, or no virus. stable flies acquired prrsv from the bloodmeal and the amount of ... | 2015 | 26336236 |
| immune responses of pigs immunized with a recombinant porcine reproductive and respiratory syndrome virus expressing porcine gm-csf. | porcine reproductive and respiratory syndrome virus (prrsv) has spread worldwide, causing huge economic losses to the swine industry. the current prrsv vaccines have failed to provide broad protection against various strains. granulocyte macrophage colony-stimulating factor (gm-csf), an efficacious adjuvant, has been shown to enhance the immunogenicity of various vaccines. the purpose of this study was to construct a recombinant live attenuated prrsv that expresses porcine gm-csf (pgm-csf) and e ... | 2015 | 26300317 |
| porcine reproductive and respiratory syndrome virus vaccine does not fit in classical vaccinology. | all vaccines are developed to elicit an effective immune response in vaccinated animals such as innate, humoral and cell mediated response to protect animal health. quality and intensity of the immune responses are differing by characteristics of the vaccine formulation and nature of the infectious agent. modified live virus vaccines showed advantages over killed vaccines in terms of rapid immune response, duration of the immunity and better cell mediated protection mechanism. the porcine reprod ... | 2015 | 26273574 |
| inhibition of porcine reproductive and respiratory syndrome virus replication in vitro using dna-based short antisense oligonucleotides. | porcine reproductive and respiratory syndrome (prrs) is caused by porcine reproductive and respiratory syndrome virus (prrsv) and is an economically important disease in swine-producing areas. the objective of this study was to screen for effective antisense oligonucleotides (as-ons) which could inhibit prrsv replication in marc-145 cells and in pulmonary alveolar macrophages (pam). | 2015 | 26265453 |
| porcine reproductive and respiratory syndrome virus 3c protease cleaves the mitochondrial antiviral signalling complex to antagonize ifn-β expression. | porcine reproductive and respiratory syndrome, a highly infectious disease caused by porcine reproductive and respiratory syndrome virus (prrsv), has developed various strategies to evade the host innate immune response, including the suppression of type i ifn activation. the mitochondrial antiviral signalling protein (mavs) is an important bridging adaptor of retinoic acid-inducible gene i/melanoma differentiation-associated protein 5 signalling pathways. here, we demonstrated that the 3c-like ... | 2015 | 26253126 |
| hp-prrsv is attenuated by de-optimization of codon pair bias in its rna-dependent rna polymerase nsp9 gene. | there is an urgent need to develop new vaccines against highly pathogenic prrs virus (hp-prrsv) variant in china. the actual use of each codon pairs is more or less frequent than that of the statistical prediction and codon pair bias (cpb) usage affects gene translation. we "shuffled" the existing codons in hp-prrsv genes gp5, m, nsp2 and nsp9, so that the cpb of these genes could be more negative. de-optimization of nsp9, the rna-dependent rna polymerase, significantly decreased prrsv replicati ... | 2015 | 26247624 |
| importation and recombination are responsible for the latest emergence of highly pathogenic porcine reproductive and respiratory syndrome virus in china. | in china, a majority of the highly pathogenic porcine reproductive and respiratory syndrome (hp-prrsv) strains were seeded by the 2006 outbreak. however, the most recently emerged (2013-2014) hp-prrsv strain has a very different genetic background. it is a nadc30-like prrsv strain recently introduced from north america that has undergone genetic exchange with the classic hp-prrsv strains in china. subsequent isolation and characterization of this variant suggest high pathogenicity, so it merits ... | 2015 | 26246582 |
| molecular epidemiology of porcine reproductive and respiratory syndrome viruses isolated from 1991 to 2013 in taiwan. | porcine reproductive and respiratory syndrome virus (prrsv) was first identified in taiwan in 1991, but the genetic diversity and evolution of prrsv has not been thoroughly investigated over the past 20 years. the aim of this study was to bridge the gap in understanding of its molecular epidemiology. a total of 31 prrsv strains were collected and sequenced. the sequences were aligned using the muscle program, and phylogenetic analysis were performed by the maximum-likelihood method and the neigh ... | 2015 | 26246243 |
| inhibition of porcine reproductive and respiratory syndrome virus replication with exosome-transferred artificial microrna targeting the 3' untranslated region. | porcine reproductive and respiratory syndrome (prrs) is an economically important swine disease. as part of the development of rna interference (rnai) strategy against the disease, in this study a recombinant adenovirus (rad) expressing the artificial microrna (amirna) targeting the 3' untranslated region (utr) was used to investigate the exosome-mediated amirna transfer from different pig cell types to porcine alveolar macrophages (pams). quantitative rt-pcr showed that the sequence-specific am ... | 2015 | 26238924 |
| porcine reproductive and respiratory syndrome virus (prrsv) inhibits rna-mediated gene silencing by targeting ago-2. | porcine reproductive and respiratory syndrome virus (prrsv) infection strongly modulates the host's immune response. the rna silencing pathway is an intracellular innate response to viral infections. however, it is unknown whether prrsv interacts with cellular rna silencing to facilitate the viral infection. here, we report for the first time the interaction between prrsv and rna silencing in both the porcine macrophages and african green monkey kidney cell line (marc-145) cell line, which were ... | 2015 | 26512690 |
| unusual severe cases of type 1 porcine reproductive and respiratory syndrome virus (prrsv) infection in conventionally reared pigs in south korea. | porcine reproductive and respiratory syndrome virus (prrsv) causes a loss of approximately us$ 70 million every year to the south korean pork industry. there are two distinct genotypes: european (type 1) and north american (type 2). in south korea, type 1 and type 2 prrsv are widely distributed and have evolved continuously since the infection was first described. here, we present two field cases of type 1 prrsv infection with unusually severe pathogenicity. | 2015 | 26497589 |
| targeting swine leukocyte antigen class i molecules for proteasomal degradation by the nsp1α replicase protein of the chinese highly pathogenic porcine reproductive and respiratory syndrome virus strain jxwn06. | porcine reproductive and respiratory syndrome virus (prrsv) is a critical pathogen of swine, and infections by this virus often result in delayed, low-level induction of cytotoxic t lymphocyte (ctl) responses in pigs. here, we report that a chinese highly pathogenic prrsv strain possessed the ability to downregulate swine leukocyte antigen class i (sla-i) molecules on the cell surface of porcine alveolar macrophages and target them for degradation in a manner that was dependent on the ubiquitin- ... | 2015 | 26491168 |
| complete genomic characterization of porcine reproductive and respiratory syndrome virus strain hb-xl. | porcine reproductive and respiratory syndrome virus (prrsv) is the causal agent of a serious disease of swine. here, we report the genome sequence of prrsv strain hb-xl isolated from a pig farm with a clinical outbreak of porcine reproductive and respiratory syndrome. the genome is 15,323 bp long and has nine open reading frames (genbank: kp162169). comparative and phylogenetic analysis showed that hb-xl belongs to the highly pathogenic prrsv (hp-prrsv) subfamily in the family prrsv. the viral n ... | 2015 | 26213972 |
| innate and adaptive immunity against porcine reproductive and respiratory syndrome virus. | many highly effective vaccines have been produced against viruses whose virulent infection elicits strong and durable protective immunity. in these cases, characterization of immune effector mechanisms and identification of protective epitopes/immunogens has been informative for the development of successful vaccine programs. diseases in which the immune system does not rapidly clear the acute infection and/or convalescent immunity does not provide highly effective protection against secondary c ... | 2015 | 26209116 |
| emergence of a virulent porcine reproductive and respiratory syndrome virus in vaccinated herds in the united states. | in early 2014, a minnesota sow farm with a solid vaccination history suffered a severe porcine reproductive and respiratory syndrome (prrs) outbreak with unusually high morbidity and mortality in piglets and sows, as well as anorexia and secondary bacterial infections in nursery pigs. due to the unusual clinical severity in a prrs-immune herd, genetic characteristics of the virus were examined to determine if a new prrsv genotype had emerged. phylogenetic analysis indicated that the virulent str ... | 2015 | 26169029 |
| characterization of three porcine reproductive and respiratory syndrome virus isolates from a single swine farm bearing strong homology to a vaccine strain. | three porcine reproductive and respiratory syndrome viruses (prrsv), nt1, nt2, and nt3, were isolated from three dying piglets from a single pig farm in jiangsu province, china. whole genome sequencing revealed that the three isolates share the highest homology with jxa1-p80, an attenuated vaccine strain developed by serial passage of highly pathogenic prrsv jxa1 in marc-145 cells. more than ten amino acids residues in orf1a, orf1b, gp4, and gp5 that were thought to be unique to jxa1 attenuated ... | 2015 | 26162970 |
| whole blood microarray analysis of pigs showing extreme phenotypes after a porcine reproductive and respiratory syndrome virus infection. | the presence of variability in the response of pigs to porcine reproductive and respiratory syndrome virus (prrsv) infection, and recent demonstration of significant genetic control of such responses, leads us to believe that selection towards more disease resistant pigs could be a valid strategy to reduce its economic impact on the swine industry. to find underlying molecular differences in prrs susceptible versus more resistant pigs, 100 animals with extremely different growth rates and viremi ... | 2015 | 26159815 |
| complete genome sequence of a novel porcine reproductive and respiratory syndrome virus that emerged in china. | a novel porcine reproductive and respiratory syndrome virus (prrsv) strain with 393 nucleotide deletions in the nonstructural protein 2 (nsp2) region was examined in this study. results will help improve our understanding of the epidemiology and genetic diversity of the north american-type prrsv in china. | 2015 | 26159524 |
| adsorption of a porcine reproductive and respiratory syndrome virus dna vaccine candidate onto biodegradable nanoparticles improves immunogenicity in mice. | porcine reproductive and respiratory syndrome (prrs) is an economically devastating viral disease of pigs. safer and more effective vaccines are urgently needed. in this study, a synthetic orf5 gene of porcine reproductive and respiratory syndrome virus (prrsv) was adsorbed onto poly(d, l-lactide-co-glycolide)/polyethylenimine (plga/pei) nanoparticles. we prepared a plga-nanoparticle-adsorbed prrsv dna vaccine and a pei-dna complex. the results showed that these model vaccines could significantl ... | 2015 | 25877819 |
| the first detection of prrsv in wild boars in slovakia. | prrsv was confirmed by a nested rt-pcr in two out of 129 samples originating from wild boars from eastern slovakia. virus isolates were genetically typed in orf7 as prrsv-1, eu-1 with 99.7% (sample 14wb) and 100% (sample 10wb) nucleotide sequence similarity to lelystad reference strain and porcilis prrs vaccine strain, respectively. the origin of prrsv in these wild boars is unclear but it is highly likely that virus was transmitted from vaccinated domestic pigs to wild boars. | 2015 | 25876282 |
| comparison of the pathogenesis of single or dual infections with type 1 and type 2 porcine reproductive and respiratory syndrome virus. | the aim of this study was to compare the pathogenicity of single or dual infections with type 1 and type 2 porcine reproductive and respiratory syndrome virus (prrsv) in pigs. pigs were inoculated intranasally with type 1 or type 2 prrsv or both viruses together. pigs infected with type 1 and type 2 prrsv together had significantly (p <0.05) fewer genomic copies of type 1 prrsv than did pigs infected with type 1 prrsv alone. pigs infected with type 2 prrsv alone or type 1 and type 2 prrsv togeth ... | 2015 | 25869916 |
| prevalence of porcine reproductive and respiratory syndrome virus detection in aborted fetuses, mummified fetuses and stillborn piglets using quantitative polymerase chain reaction. | the objective of the present study was to investigate the prevalence of porcine reproductive and respiratory syndrome (prrs) virus detection in aborted fetuses (n=32), mummified fetuses (n=30) and stillborn piglets (n=27) from 10 swine herds in thailand using quantitative polymerase chain reaction (qpcr). pooled organs and umbilical cord from each fetus/piglet were homogenized and subjected to rna extraction and cdna synthesis. the qpcr was carried out on the orf7 of the prrs viral genome using ... | 2015 | 25866409 |
| difference in microrna expression and editing profile of lung tissues from different pig breeds related to immune responses to hp-prrsv. | porcine reproductive and respiratory syndrome (prrs) is one of the most devastating diseases for the pig industry. our goal was to identify micrornas involved in the host immune response to prrs. we generated microrna expression profiles of lung tissues from tongcheng or landrace pigs infected with a highly pathogenic prrs virus (prrsv) at 3, 5, 7 dpi (day post infection) and control individuals from these two breeds. our data showed that 278 known and 294 novel micrornas were expressed in these ... | 2015 | 25856272 |
| comparison of two commercial type 1 porcine reproductive and respiratory syndrome virus (prrsv) modified live vaccines against heterologous type 1 and type 2 prrsv challenge in growing pigs. | the objective of the present study was to compare the efficacy of two commercial type 1 porcine reproductive and respiratory syndrome virus (prrsv) modified live vaccines against heterologous type 1 and type 2 prrsv challenge in growing pigs. vaccination with a type 1 prrsv vaccine reduced the level of viremia after type 1 prrsv challenge but did not reduce the level of viremia after the type 2 prrsv challenge in pigs. increased levels of interleukin-10 (il-10) stimulated by type 2 prrsv coincid ... | 2015 | 25855554 |
| enhanced detection of porcine reproductive and respiratory syndrome virus in fixed tissues by in situ hybridization following tyramide signal amplification. | this study evaluated the sensitivity of biotinyl-tyramide-based in situ hybridization (tish) method by comparison with chromogenic in situ hybridization (cish) and immunohistochemical staining (ihc) methods. this study also determined the effect of fixative and fixation time on the detection of porcine reproductive and respiratory syndrome virus (prrsv) in paraffin-embedded tissues. lung samples were fixed in 4% paraformaldehyde (pfa) or 10% neutral buffered formalin (nbf) for various times befo ... | 2015 | 25855364 |
| broadly neutralizing antibodies against the rapidly evolving porcine reproductive and respiratory syndrome virus. | neutralizing antibodies are a critical part of the immune armory for defense against viruses, and the mechanism by which many effective vaccines work to protect against viral infections. however, infections by rapidly evolving and genetically diverse viruses are often characterized by ineffective neutralizing antibody responses. porcine reproductive and respiratory syndrome virus (prrsv) is a highly genetically diverse rna virus that causes prrs, the most significant disease of pigs worldwide. t ... | 2015 | 25845944 |
| porcine reproductive and respiratory syndrome virus (prrsv) modified-live vaccine reduces virus transmission in experimental conditions. | some vaccination strategies have shown good results in reducing the clinical outcomes of prrs. nevertheless the effect of vaccines on viral transmission is poorly described, so we aimed to fill this gap with the present study. twelve specific pathogen free (spf) piglets, vaccinated against prrsv at 3 weeks of age (porcilis prrs id(®), msd), were inoculated at 31 days post-vaccination with a heterologous genogroup 1.1 strain, and put in contact with 12 vaccinated piglets during 49 days. the same ... | 2015 | 25820061 |
| the cholera toxin b subunit (ctb) fused to the porcine arterivirus matrix m and gp5 envelope proteins fails to enhance the gp5-specific antibody response in pigs immunized with adenovectors. | the porcine reproductive and respiratory syndrome virus (prrsv) is an arterivirus of the arteriviridae family. as the current commercial vaccines are incompletely protective effective against prrsv infection, we developed a vaccine strategy using replicating but non-disseminating adenovectors (radvs) expressing the prrsv m matrix protein in fusion with the neutralizing major epitope-carrying gp5 envelope protein (roques et al. in vet res 44:17, 2013). although production of gp5-specific antibodi ... | 2015 | 25801418 |
| pathogenicity of three type 2 porcine reproductive and respiratory syndrome virus strains in experimentally inoculated pregnant gilts. | mechanisms of reproductive failure resulting from infection with porcine reproductive and respiratory syndrome virus (prrsv) are still poorly understood. presented herein are the results of a side-by-side evaluation of the pathogenicity of three type 2 prrsv strains in a reproductive model, from a pilot study used to develop experimental conditions and laboratory methods for a larger experiment. pregnant gilts were experimentally infected with prrsv at gestation day 85 or served as uninfected ne ... | 2015 | 25796212 |
| draco inhibits porcine reproductive and respiratory syndrome virus replication in vitro. | porcine reproductive and respiratory syndrome virus (prrsv) continues to cause substantial economic losses to the pig industry worldwide. current vaccination strategies and antiviral drugs against prrsv are still inadequate. therefore, there is an urgent need for new antiviral strategies to control prrsv. double-stranded rna (dsrna) activated caspase oligomerizer (draco) is a synthetic construct consisting of a dsrna detection domain, an apoptosis induction domain, and a transduction tag. it has ... | 2015 | 25772577 |
| cross-protection of a new type 2 porcine reproductive and respiratory syndrome virus (prrsv) modified live vaccine (fostera prrs) against heterologous type 1 prrsv challenge in growing pigs. | the objective of the present study was to determine the cross-protection of a new type 2 porcine reproductive and respiratory syndrome virus (prrsv) modified live vaccine against heterologous type 1 prrsv challenge in growing pigs. the mean rectal temperature and respiratory score was significantly (p<0.05) lower in vaccinated challenged pigs than in unvaccinated challenged pigs. vaccination of pigs with type 2 prrsv reduced the levels of type 1 prrsv viremia after challenge with type 1 prrsv. v ... | 2015 | 25769646 |
| porcine reproductive and respiratory syndrome virus nonstructural protein 2 (nsp2) topology and selective isoform integration in artificial membranes. | the membrane insertion and topology of nonstructural protein 2 (nsp2) of porcine reproductive and respiratory syndrome virus (prrsv) strain vr-2332 was assessed using a cell free translation system in the presence or absence of artificial membranes. expression of prrsv nsp2 in the absence of all other viral factors resulted in the genesis of both full-length nsp2 as well as a select number of c-terminal nsp2 isoforms. addition of membranes to the translation stabilized the translation reaction, ... | 2015 | 25768891 |
| development of a genome copy specific rt-qpcr assay for divergent strains of type 2 porcine reproductive and respiratory syndrome virus. | porcine reproductive and respiratory syndrome virus (prrsv) became a significant pathogen of swine upon its emergence in the late 1980s and since then has exemplified a rapidly evolving, constantly re-emerging pathogen. in addition to the challenges faced in development of vaccines and diagnostics, research on the basic molecular pathogenesis of prrsv is also restrained by the ability to accurately and comparatively quantitate levels of replication in different tissues and between strains. this ... | 2015 | 25766790 |
| a porcine reproductive and respiratory syndrome virus vaccine candidate based on prrsv glycoprotein 5 and the toll-like receptor 5 agonist salmonella typhimurium flagellin. | glycoprotein 5 (gp5) from porcine reproductive and respiratory syndrome virus (prrsv) is a key inducer of neutralizing antibodies. a truncated gp5 gene lacking the signal peptide and transmembrane sequences was amplified via an overlap pcr method and inserted into prokaryotic expression vectors, pet32a or pgex-6p-1, to add an his or gst tag, respectively. his-tagged gp5 was induced with iptg, verified by sds-page and western blotting, and purified to serve as an immunogen accompanied with the sa ... | 2015 | 25766593 |
| pd-l1 expression is increased in monocyte derived dendritic cells in response to porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus infections. | host immune system suppression is thought to be crucial in the development of porcine circovirus associated diseases (pcvad). many immune suppressive mechanisms have been studied in cases of pcvad, however, the role of programmed death ligand-1 (pd-l1) during porcine circovirus type 2 (pcv2) infection and pcvad development has yet to be determined. pd-l1 has become an important research target because of its ability to interfere with effective t-cell activity and proliferation during the course ... | 2015 | 26553563 |
| multiplex serology for common viral infections in feral pigs (sus scrofa) in hawaii between 2007 and 2010. | multiplex serology was performed for the detection of total immunoglobulin (ig) and igm antibodies against porcine circovirus type 2 (pcv2), porcine reproductive and respiratory syndrome virus (prrsv), and swine influenza virus (siv) antigens in feral swine (sus scrofa). serum samples were collected from the islands of oahu (292 pigs) and hawaii (52 pigs) between 2007 and 2010. the highest antibody prevalence was to pcv2 (63%), followed by siv (7.8%) and prrsv (5.8%). antigen-specific igm was de ... | 2015 | 25380357 |
| the pd-l1/cd86 ratio is increased in dendritic cells co-infected with porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus, and the pd-l1/pd-1 axis is associated with anergy, apoptosis, and the induction of regulatory t-cells in porcine lymphocytes. | porcine circovirus type 2 (pcv2) and porcine reproductive and respiratory syndrome virus (prrsv) continue to have a negative economic impact on global swine production operations. host immune modulations that potentiate disease during pcv2 and/or prrsv infections are important areas of ongoing research. in this study, we evaluated the expression levels of pd-l1, cd86, and il-10 in order to phenotype dendritic cells following viral infection with pcv2b and/or prrsv. the results showed that the in ... | 2015 | 26446939 |
| vaccination with a porcine reproductive and respiratory syndrome (prrs) modified live virus vaccine followed by challenge with prrs virus and porcine circovirus type 2 (pcv2) protects against prrs but enhances pcv2 replication and pathogenesis compared to results for nonvaccinated cochallenged controls. | coinfections involving porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2) contribute to a group of disease syndromes known as porcine circovirus-associated disease (pcvad). presumably, prrsv infection enhances pcv2 replication as a result of modulation of host immunity. the purpose of this study was to evaluate pcv2 replication and pathogenesis in pigs vaccinated with a prrs modified live virus (mlv) vaccine and subsequently challenged with a combina ... | 2015 | 26446422 |
| development of real-time pcr assay for detection of porcine circovirus-like virus p1 in domestic pigs in china. | the porcine circovirus-like agent p1 is a newly discovered dna virus with a single-stranded circular genome that is highly homologous to that of porcine circovirus type 2. p1 infection can cause symptoms resembling postweaning multisystemic wasting syndrome. this study aims to develop a rapid, sensitive and specific method to detect p1. | 2015 | 26404908 |
| diagnostic accuracy of porcine acute phase proteins in meat juice for detecting disease at abattoir. | the aim of this work was to evaluate whether acute phase protein (app) determinations could assist official veterinarians carrying out work in slaughterhouses. to test this hypothesis, the diagnostic accuracy of app determinations in meat juice of pigs was analysed to differentiate between healthy and diseased pigs. one hundred and one pigs of two different origins were classified into two groups according to their health status (healthy and diseased pigs), which was determined by a veterinary c ... | 2015 | 26101294 |
| expression of toll-like receptor signaling-related genes in pigs co-infected with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. | pigs co-infected with porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2) have been shown to develop more severe diseases than pigs infected with prrsv or pcv2 only. the underlying interaction mechanisms between the two viruses in developing the disease are unclear. the present study investigates the mrna expression of toll-like receptor (tlr) signaling-related molecules in peripheral blood mononuclear cells from pigs infected with prrsv or pcv2 or bo ... | 2015 | 26022073 |
| [construction and identification of a recombinant prrsv expressing orf2 of porcine circovirus type 2]. | porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2) are very two important pathogens that have coursed huge economic losses in swine production in worldwide. in this study,a vector pcmv-tjm containing the full-length cdna clone of prrsv attenuated strain tjm-f92 was firstly constructed by pcr method. then a gene sequence containing afl ii/mlu i e restriction enzyme sites and a transcription regulatory sequence for orf6 (trs6) was inserted be- tween or ... | 2015 | 25997333 |
| expression profile of toll-like receptor mrna in pigs co-infected with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. | field and experimental studies have shown that co-infection of pigs with porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2) increases the severity of the disease. the present study investigates the mrna expression profile of toll-like receptors (tlrs) in pigs co-infected with prrsv and pcv2. spf pigs were infected with prrsv, pcv2 or in a combination of both. the mrna expression levels of tlrs and related cytokines in peripheral blood mononuclear cel ... | 2015 | 25555603 |
| efficacy of combined vaccination against mycoplasma hyopneumoniae and porcine reproductive and respiratory syndrome virus in dually infected pigs. | porcine respiratory disease complex (prdc) is one of the main causes of economic losses for swine producers. this complex is due to a combination of different pathogens and their interactions. two major pathogens involved in prdc are mycoplasma hyopneumoniae (mhp) and porcine reproductive and respiratory syndrome virus (prrsv). the objectives of this study were (i) to develop an experimental model of dual mhp/prrsv infection in spf pigs with european strains of mhp and prrsv and (ii) to assess a ... | 2015 | 26422712 |
| genetic association of the porcine c9 complement component with hemolytic complement activity. | the complement system is a part of the natural immune regulation mechanism against invading pathogens. complement activation from three different pathways (classical, lectin, and alternative) leads to the formation of c5-convertase, an enzyme for cleavage of c5 into c5a and c5b, followed by c6, c7, c8, and c9 in membrane attack complex. the c9 is the last complement component of the terminal lytic pathway, which plays an important role in lysis of the target cells depending on its self-polymeriz ... | 2015 | 26194222 |
| transcription analysis on response of porcine alveolar macrophages to co-infection of the highly pathogenic porcine reproductive and respiratory syndrome virus and mycoplasma hyopneumoniae. | porcine respiratory disease complex (prdc) is of great concern economically, for swine producers worldwide. co-infections with porcine reproductive and respiratory syndrome virus (prrsv) and mycoplasma hyopneumoniae (mhp) are considered the major causative agents of prdc, and responsible for mass mortality in pigs. nevertheless, the molecular mechanisms underlying the host factors involved in pathogenesis and persistent infection have not been clearly established because of a lack of information ... | 2015 | 25445346 |
| first identification of porcine parvovirus 6 in north america by viral metagenomic sequencing of serum from pigs infected with porcine reproductive and respiratory syndrome virus. | currently, eight species in four genera of parvovirus have been described that infect swine. these include ungulate protoparvovirus 1 (classical porcine parvovirus, ppv), ungulate tetraparvovirus 2 (ppv3), ungulate tetraparvovirus 3 (which includes ppv2, porcine hokovirus, porcine partetravirus and porcine parv4), ungulate copiparvovirus 2 (which includes ppv4 and ppv5), ungulate bocaparvovirus 2 (which includes porcine bocavirus 1, 2 and 6), ungulate bocaparvovirus 3 (porcine bocavirus 5), ungu ... | 2015 | 26475593 |
| the pathogenic role of torque teno sus virus 1 and 2 and their correlations with various viral pathogens and host immunocytes in wasting pigs. | the pathogenic role of torque teno sus virus (ttsuv) in swine is controversial among different studies. the present study intended to evaluate the potential pathogenicity of ttsuv based on its correlations with the histopathological changes, various common concurrently infected viral pathogens including porcine circovirus type 2 (pcv2), porcine reproductive and respiratory syndrome virus (prrsv), and porcine parvovirus (ppv), as well as changes in the distribution and population of host immunocy ... | 2015 | 26390821 |
| expression of antigenic epitopes of porcine reproductive and respiratory syndrome virus (prrsv) in a modified live-attenuated porcine circovirus type 2 (pcv2) vaccine virus (pcv1-2a) as a potential bivalent vaccine against both pcv2 and prrsv. | co-infection of pigs in the field with porcine circovirus type 2 (pcv2) and porcine reproductive and respiratory syndrome virus (prrsv) is common and poses a major concern in effective control of pcv2 and prrsv. we previously demonstrated that insertion of foreign epitope tags in the c-terminus of pcv2 orf2 produced infectious virions that elicited humoral immune responses against both pcv2 capsid and inserted epitope tags. in this study, we aimed to determine whether the non-pathogenic chimeric ... | 2015 | 26239318 |
| application of a pathogen microarray for the analysis of viruses and bacteria in clinical diagnostic samples from pigs. | many of the disease syndromes challenging the commercial swine industry involve the analysis of complex problems caused by polymicrobial, emerging or reemerging, and transboundary pathogens. this study investigated the utility of the lawrence livermore microbial detection array (lawrence livermore national laboratory, livermore, california), designed to detect 8,101 species of microbes, in the evaluation of known and unknown microbes in serum, oral fluid, and tonsil from pigs experimentally coin ... | 2015 | 25855363 |
| complete genomic characterization of a porcine reproductive and respiratory syndrome virus isolate in xinjiang province of china. | porcine reproductive and respiratory syndrome (prrs) is a swine disease of major economic importance that causes reproductive and respiratory problems in pigs. in the present study, one strain of porcine reproductive and respiratory syndrome virus (prrsv) was isolated in xinjiang province, northwest china, designated xju-1. the full-length genome of xju-1 was found to be 14,987 nucleotides in length, including the poly(a) tail. comparative analysis with the genomic sequences of type 2 isolates r ... | 2015 | 25272960 |
| comparative analysis of cytokine transcript profiles within mediastinal lymph node compartments of pigs after infection with porcine reproductive and respiratory syndrome genotype 1 strains differing in pathogenicity. | porcine reproductive and respiratory syndrome virus (prrsv) induces a weak immune response enabling it to persist in different organs of infected pigs. this has been attributed to the ability of prrsv to influence the induction of cytokine responses. in this study, we investigated the cytokine transcriptional profiles in different compartments of the mediastinal lymph node of pigs infected with three genotype 1 prrsv strains of differing pathogenicity: the low virulence prototype lelystad virus ... | 2015 | 25889072 |
| diagnosis of the lelystad strain of porcine reproductive and respiratory syndrome virus infection in individually housed pigs: comparison between serum and oral fluid samples for viral nucleic acid and antibody detection. | there has been a developing interest in the use of oral fluid for the diagnosis of different pathogens such as porcine reproductive and respiratory syndrome virus (prrsv). prrsv and prrsv-specific antibodies have been shown to be present in oral fluid samples, but the correlation between diagnostic results in oral fluid and serum samples has been insufficiently addressed. studies investigating this correlation focused on boars older than 6 months and type 2 strains, but it is known that the outc ... | 2015 | 25525137 |
| domestic pigs are susceptible to infection with influenza b viruses. | influenza b virus (ibv) causes seasonal epidemics in humans. although ibv has been isolated from seals, humans are considered the primary host and reservoir of this important pathogen. it is unclear whether other animal species can support the replication of ibv and serve as a reservoir. swine are naturally infected with both influenza a and c viruses. to determine the susceptibility of pigs to ibv infection, we conducted a serological survey for u.s. midwest domestic swine herds from 2010 to 20 ... | 2015 | 25673727 |
| inhibitory effects of lithium chloride on replication of type ii porcine reproductive and respiratory syndrome virus in vitro. | porcine reproductive and respiratory syndrome (prrs) is one of the most serious diseases affecting the swine industry worldwide; however, there are no efficient control strategies against prrs virus (prrsv) at present. therefore, development of new antiviral treatment strategies is urgently needed. as reported, lithium chloride (licl) can efficiently impair the replication of a variety of viruses, including infectious bronchitis coronavirus (ibv) and transmissible gastroenteritis coronavirus (tg ... | 2015 | 25560301 |
| concentration, size distribution, and infectivity of airborne particles carrying swine viruses. | when pathogens become airborne, they travel associated with particles of different size and composition. particle size determines the distance across which pathogens can be transported, as well as the site of deposition and the survivability of the pathogen. despite the importance of this information, the size distribution of particles bearing viruses emitted by infectious animals remains unknown. in this study we characterized the concentration and size distribution of inhalable particles that ... | 2015 | 26287616 |
| association of the presence of influenza a virus and porcine reproductive and respiratory syndrome virus in sow farms with post-weaning mortality. | influenza a virus (iav) and porcine reproductive and respiratory syndrome virus (prrsv) are among the most important pathogens affecting pigs worldwide. their effect on post-weaning mortality can be substantial and may be potentiated by other concomitant factors. here, the objective was to evaluate the association between iav and prrsv infection at weaning with post-weaning mortality observed in wean-to-finish farms in order to better quantify the full impact of their presence in breeding herds. ... | 2015 | 26210012 |
| function of cd163 fragments in porcine reproductive and respiratory syndrome virus infection. | monocyte/macrophage scavenger receptor cd163 plays an important role in porcine reproductive and respiratory syndrome virus (prrsv) infection. to identify the domains of cd163 involved in prrsv infection, cd163 fragments p1 (1-798 bp), p2 (790-2046 bp), p3 (2023-3345 bp), y-p1 (160-798 bp), y-p2 (790-2046 bp) and y-p3 (2143-3084 bp) were expressed by eukaryotic and prokaryotic expression systems, respectively. infection experiments revealed that non-permissive bhk-21 cells transfected with pcd16 ... | 2015 | 26629026 |
| hepatitis e virus chronic infection of swine co-infected with porcine reproductive and respiratory syndrome virus. | in developed countries, most of hepatitis e human cases are of zoonotic origin. swine is a major hepatitis e virus (hev) reservoir and foodborne transmissions after pork product consumption have been described. the risk for hev-containing pig livers at slaughter time is related to the age at infection and to the virus shedding duration. porcine reproductive and respiratory syndrome virus (prrsv) is a virus that impairs the immune response; it is highly prevalent in pig production areas and suspe ... | 2015 | 26048774 |
| immune response of porcine alveolar macrophages to a concurrent infection with porcine reproductive and respiratory syndrome virus and haemophilus parasuis in vitro. | porcine reproductive and respiratory syndrome virus (prrsv) can predispose pigs to secondary respiratory infection with bacteria such as haemophilus parasuis. animals infected with both pathogens develop more severe clinical disease. the immune response of porcine alveolar macrophages (pams) to simultaneous infection with prrsv and h. parasuis was analysed in vitro, describing cytokine production, expression of cell surface molecules, and production of reactive oxygen species (ros). concurrent i ... | 2015 | 26358898 |
| associations among haemophilus parasuis, mycoplasma hyorhinis, and porcine reproductive and respiratory syndrome virus infections in pigs with polyserositis. | the objective of this study was to determine the associations among haemophilus parasuis, mycoplasma hyorhinis, and porcine reproductive and respiratory syndrome (prrs) virus (eu-field strain) infections in 95 pigs with polyserositis. a significant association between h. parasuis and m. hyorhinis was identified. h. parasuis and m. hyorhinis were significantly more often detected in prrs virus positive pigs. | 2015 | 25750450 |
| development and evaluation of a polydiacetylene based biosensor for the detection of h5 influenza virus. | h5n1 avian influenza has caused serious economic losses as well as posed significant threats to public health, agriculture and wildlife. it is important to develop a rapid, sensitive and specific detection platform suitable for disease surveillance and control. in this study, a highly sensitive, specific and rapid biosensor based on polydiacetylene was developed for detecting h5 influenza virus. the polydiacetylene based biosensor was produced from an optimized ratio of 10,12-pentacosadiynoic ac ... | 2015 | 25819686 |
| partial sequence of a novel virus isolated from pelodiscus sinensis hemorrhagic disease. | outbreaks of hemorrhagic syndrome-like disease with high mortality rates have frequently occurred in pelodiscus sinensis farms. the purpose of this study was to investigate the pathogen through challenge infection assays and partial sequencing of the genome of the pathogen. a 453-bp amplicon was obtained by random pcr using the nucleic acid extracted from the tissue homogenate filtrate and showed 32% identity at the amino acid level with the replicase polyprotein of the porcine reproductive and ... | 2015 | 26279281 |
| in vivo assessment of equine arteritis virus vaccine improvement by disabling the deubiquitinase activity of papain-like protease 2. | arteriviruses are a family of positive-stranded rna viruses that includes the prototypic equine arteritis virus (eav) and porcine reproductive and respiratory syndrome virus (prrsv). although several vaccines against these viruses are commercially available there is room for improvement, especially in the case of prrsv. the ability of arteriviruses to counteract the immune response is thought to decrease the efficacy of the current modified live virus vaccines. we have recently shown that the de ... | 2015 | 25975520 |
| proteolytic processing of the porcine reproductive and respiratory syndrome virus replicase. | the porcine reproductive and respiratory syndrome virus (prrsv) replicase polyproteins pp1a and pp1ab are proteolytically processed by four proteases encoded in orf1a. in this study, a large set of prrsv replicase cleavage products were identified and pp1a cleavage sites were verified by using a combination of bioinformatics, proteomics, immunoprecipitation, and site-directed mutagenesis. for genotype 1 prrsv (isolate sd01-08), proteomic analysis identified h180/s181, g385/a386, and g1446/a1447 ... | 2015 | 25557977 |
| different clinical, virological, serological and tissue tropism outcomes of two new and one old belgian type 1 subtype 1 porcine reproductive and respiratory virus (prrsv) isolates. | in this study, the pathogenic behavior of prrsv 13v091 and 13v117, isolated in 2013 from two different belgian farms with enzootic respiratory problems shortly after weaning in the nursery, were compared with the belgian strain 07v063 isolated in 2007. full-length genome sequencing was performed to identify their origin. twelve weeks-old pigs were inoculated intranasally (in) with 13v091, 13v117 or 07v063 (9 pigs/group). at 10 days post inoculation (dpi), 4 animals from each group were euthanize ... | 2015 | 25885416 |
| evaluation of an electrostatic particle ionization technology for decreasing airborne pathogens in pigs. | influenza a virus (iav), porcine reproductive and respiratory syndrome virus (prrsv), porcine epidemic diarrhea virus (pedv) and staphylococcus aureus are important swine pathogens capable of being transmitted via aerosols. the electrostatic particle ionization system (epi) consists of a conductive line that emits negative ions that charge particles electrically resulting in the settling of airborne particles onto surfaces and potentially decreasing the risk of pathogen dissemination. the object ... | 2015 | 27616810 |
| rapid and specific detection of porcine parvovirus using real-time pcr and high resolution melting (hrm) analysis. | porcine parvovirus (ppv) is the important causative agent for infectious infertility, which is a fairly tough virus that multiplies normally in the intestine of pigs without causing clinical signs in the world. | 2015 | 25879634 |
| encephalomyocarditis virus 3c protease relieves traf family member-associated nf-κb activator (tank) inhibitory effect on traf6-mediated nf-κb signaling through cleavage of tank. | traf family member-associated nf-κb activator (tank) is a negative regulator of canonical nf-κb signaling in the toll-like receptor- and b-cell receptor-mediated signaling pathways. however, functions of tank in viral infection-mediated nf-κb activation remain unclear. here, we reported that tank was cleaved by encephalomyocarditis virus 3c at the 197 and 291 glutamine residues, which depends on its cysteine protease activity. in addition, encephalomyocarditis virus 3c impaired the ability of ta ... | 2015 | 26363073 |
| induction of ros generation and nf-κb activation in marc-145 cells by a novel porcine reproductive and respiratory syndrome virus in southwest of china isolate. | porcine reproductive and respiratory syndrome virus (prrsv) is the cause of an economically important swine disease that has devastated the swine industry since the late 1980s. the aim of the present study was to investigate the interaction between reactive oxygen species (ros) and nf-κb by prrsv infection. | 2015 | 26358082 |
| microrna mir-24-3p promotes porcine reproductive and respiratory syndrome virus replication through suppression of heme oxygenase-1 expression. | porcine reproductive and respiratory syndrome virus (prrsv) is one of the most economically important viruses affecting the swine industry worldwide. our previous research showed that prrsv downregulates the expression of heme oxygenase-1 (ho-1), a pivotal cytoprotective enzyme, postinfection and that overexpression of ho-1 inhibits prrsv replication. micrornas regulate gene expression at the posttranscriptional level and have recently been demonstrated to play vital roles in pathogen-host inter ... | 2015 | 25653454 |
| heme oxygenase-1 suppresses bovine viral diarrhoea virus replication in vitro. | viral cycle progression depends upon host-cell processes in infected cells, and this is true for bovine viral diarrhoea virus (bvdv), the causative agent of bvd that is a worldwide threat to the bovine industry. heme oxygenase-1 (ho-1) is a ubiquitously expressed inducible isoform of the first and rate-limiting enzyme for heme degradation. recent studies have demonstrated that ho-1 has significant antiviral properties, inhibiting the replication of viruses such as ebola virus, human immunodefici ... | 2015 | 26510767 |
| exploration of new sites in adenovirus hexon for foreign peptides insertion. | adenoviral vectors are now being explored as vaccine carriers to prevent infectious diseases in humans and animals. there are two strategies aimed at the expression of a vaccine antigen by adenoviral vectors. the first includes an insertion of the foreign gene expression cassette into the e1 region. the second strategy is antigen incorporation into the viral capsid proteins. to extend this methodology, we have searched for new sites at the human adenovirus serotype 5 major capsid protein hexon f ... | 2015 | 26069516 |
| single-chain anti-idiotypic antibody retains its specificity to porcine reproductive and respiratory syndrome virus gp5. | monoclonal anti-idiotypic antibody (mab2-5g2) raised against idiotypic antibodies to membrane glycoprotein gp5 of porcine reproductive and respiratory syndrome virus (prrsv). the variable regions of the heavy chain (vh) and light chain (vl) of mab2-5g2 were cloned and connected with a (gly4ser)3 linker. the recombinant scfv gene was cloned into the peasy-e1 vector and expressed in e. coli as inclusion bodies. the expressed scfv-his proteins renatured in a ph and urea gradient buffer retained the ... | 2015 | 25448704 |
| analysis of ttsuv1b antibody in porcine serum and its correlation with four antibodies against common viral infectious diseases. | the purpose of the present study was to evaluate the correlation between torque teno sus virus 1b (ttsuv1b) infection and other viral infections or vaccine immunization in conventional pigs. | 2015 | 26260234 |
| simultaneous detection of eight swine reproductive and respiratory pathogens using a novel gexp analyser-based multiplex pcr assay. | a new high-throughput genomelab gene expression profiler (gexp) analyser-based multiplex pcr assay was developed for the detection of eight reproductive and respiratory pathogens in swine. the reproductive and respiratory pathogens include north american porcine reproductive and respiratory syndrome virus (prrsv-na), classical swine fever virus (csfv), porcine circovirus 2 (pcv-2), swine influenza virus (siv) (including h1 and h3 subtypes), porcine parvovirus (ppv), pseudorabies virus (prv) and ... | 2015 | 26259690 |
| recombinant encephalomyocarditis viruses elicit neutralizing antibodies against prrsv and csfv in mice. | encephalomyocarditis virus (emcv) is capable of infecting a wide range of species and the infection can cause myocarditis and reproductive failure in pigs as well as febrile illness in human beings. in this study, we introduced the entire orf5 of the porcine reproductive and respiratory syndrome virus (prrsv) or the neutralization epitope regions in the e2 gene of the classical swine fever virus (csfv), into the genome of a stably attenuated emcv strain, t1100i. the resultant viable recombinant ... | 2015 | 26076449 |
| fatal disease associated with swine hepatitis e virus and porcine circovirus 2 co-infection in four weaned pigs in china. | in recent decades, porcine circovirus 2 (pcv2) infection has been recognized as the causative agent of postweaning multisystemic wasting syndrome, and has become a threat to the swine industry. hepatitis e virus (hev) is another high prevalent pathogen in swine in many regions of the world. pcv2 and hev are both highly prevalent in pig farms in china. | 2015 | 25889526 |
| a single amino acid deletion in the matrix protein of porcine reproductive and respiratory syndrome virus confers resistance to a polyclonal swine antibody with broadly neutralizing activity. | assessment of virus neutralization (vn) activity in 176 pigs infected with porcine reproductive and respiratory syndrome virus (prrsv) identified one pig with broadly neutralizing activity. a tyr-10 deletion in the matrix protein provided escape from broad neutralization without affecting homologous neutralizing activity. the role of the tyr-10 deletion was confirmed through an infectious clone with a tyr-10 deletion. the results demonstrate differences in the properties and specificities of vn ... | 2015 | 25855739 |
| actinobacillus pleuropneumoniae induces sjpl cell cycle arrest in g2/m-phase and inhibits porcine reproductive and respiratory syndrome virus replication. | porcine reproductive and respiratory syndrome virus (prrsv) is one of the most important pathogens in the swine industry and causes important economic losses. no effective antiviral drugs against it are commercially available. we recently reported that the culture supernatant of actinobacillus pleuropneumoniae, the porcine pleuropneumonia causative agent, has an antiviral activity in vitro against prrsv in sjpl cells. objectives of this study were (i) to identify the mechanism behind the antivir ... | 2015 | 26577697 |
| erythema multiforme associated with respiratory disease in a commercial breeding pig herd. | this study describes an erythema multiforme (em) in breeding sows, after their mixing in the group housing system. sows at 30-35 days of gestation showed red and raised skin areas, depression, anorexia, fever, respiratory problems, and increased return to estrus. blood and nasal samples from diseased sows were examined by quantitative polymerase chain reaction and enzyme-linked immunosorbent assay for respiratory pathogens. hematological and biochemical analyses were performed on the blood sampl ... | 2015 | 26266696 |
| exposure of extensively farmed wild boars (sus scrofa scrofa) to selected pig pathogens in greece. | increased density and distribution of wild boar populations are likely to promote interactions and transmission of certain pathogens, not only among wild boar but also from wild boar to livestock or humans and vice versa. | 2015 | 25774434 |
| a multiplex pcr for simultaneous detection of classical swine fever virus, african swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines. | in this assay, we developed and evaluated a multiplex pcr (mpcr) for its ability in detecting multiple infections of swine simultaneously. four pairs of primers were used to detect five viruses. specific primers were designed for classical swine fever virus (csfv), african swine fever virus (asfv) and pseudorabies (prv). a pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respirato ... | 2015 | 26812812 |
| maternal and fetal predictors of fetal viral load and death in third trimester, type 2 porcine reproductive and respiratory syndrome virus infected pregnant gilts. | minimal research has focused on understanding mechanisms underlying porcine reproductive and respiratory syndrome virus (prrsv) induced reproductive failure. we have completed a large-scale project investigating phenotypic and genotypic predictors of reproductive prrs severity in which numerous clinical, pathological, immunologic and viral responses were characterized in dams and fetuses. the goal was to determine which phenotypic responses were associated with fetal viral load and death after e ... | 2015 | 26407558 |
| a serosurvey for selected pathogens in greek european wild boar. | serum samples, collected from 94 european wild boar (sus scrofa) during the hunting seasons 2006 -2010 from different regions of greece, were examined in order to estimate the role of these wildlife species as reservoir of pathogens important for livestock and/or public health. | 2015 | 26392908 |
| identification of a putative quantitative trait nucleotide in guanylate binding protein 5 for host response to prrs virus infection. | previously, we identified a major quantitative trait locus (qtl) for host response to porcine respiratory and reproductive syndrome virus (prrsv) infection in high linkage disequilibrium (ld) with snp rs80800372 on sus scrofa chromosome 4 (ssc4). | 2015 | 26016888 |
| early postnatal respiratory viral infection induces structural and neurochemical changes in the neonatal piglet brain. | infections that cause inflammation during the postnatal period are common, yet little is known about their impact on brain development in gyrencephalic species. to address this issue, we investigated brain development in domestic piglets which have brain growth and morphology similar to human infants, after experimentally infecting them with porcine reproductive and respiratory syndrome virus (prrsv) to induce an interstitial pneumonia piglets were inoculated with prrsv on postnatal day (pd) 7 a ... | 2015 | 25967923 |
| deciphering transcriptome profiles of peripheral blood mononuclear cells in response to prrsv vaccination in pigs. | porcine reproductive and respiratory syndrome (prrs) is one of the most economically important viral diseases affecting swine industry worldwide. despite routine farm vaccination, effective control strategies for prrs remained elusive which underscores the need for in-depth studies to gain insight into the host immune response to vaccines. the current study aimed to investigate transcriptional responses to prrs virus (prrsv) vaccine in the peripheral blood mononuclear cells (pbmcs) within 3 days ... | 2016 | 27528396 |
| safety and early onset of immunity with a novel european porcine reproductive and respiratory syndrome virus vaccine in young piglets. | porcine reproductive and respiratory syndrome virus (prrsv) can be difficult to manage in commercial settings. a novel type i prrsv vaccinal strain (94881) was evaluated for safety and efficacy/onset of immunity (ooi) in piglets. in 2 experiments, groups of piglets were vaccinated intramuscularly (im) at approximately 14 d of age with a maximum-range commercial dose, an overdose, or a placebo in experiment 1 and either a minimum-range commercial dose or a placebo in experiment 2. the piglets in ... | 2016 | 27127339 |
| identification of mirnomes reveals ssc-mir-30d-r_1 as a potential therapeutic target for prrs viral infection. | porcine reproductive and respiratory syndrome virus (prrsv) is known to cause reproductive disorders, such as abortion, in pregnant sows as well as immunosuppressive respiratory complications, leading to severe respiratory tract infections in young pigs. in this study, an in-depth analysis of the mirnomes in mock- and virus-infected pig lungs was carried out. we found that highly expressed ssc-mir-30d-r_1 was decreased in infected lungs, and reduced levels were significantly correlated with infe ... | 2016 | 27117627 |
| bioinformatic analyses in early host response to porcine reproductive and respiratory syndrome virus (prrsv) reveals pathway differences between pigs with alternate genotypes for a major host response qtl. | a region on sus scrofa chromosome 4 (ssc4) surrounding single nucleotide polymorphism (snp) marker wur10000125 (wur) has been reported to be strongly associated with both weight gain and serum viremia in pigs after infection with prrs virus (prrsv). a proposed causal mutation in the guanylate binding protein 5 gene (gbp5) is predicted to truncate the encoded protein. to investigate transcriptional differences between wur genotypes in early host response to prrsv infection, an rna-seq experiment ... | 2016 | 26951612 |
| a genome-wide association study of fetal response to type 2 porcine reproductive and respiratory syndrome virus challenge. | control of porcine reproductive and respiratory syndrome (prrs) is economically important for the swine industry worldwide. as current prrs vaccines do not completely protect against heterologous challenge, alternative means of control, including enhanced genetic resilience, are needed. for reproductive prrs, the genetic basis of fetal response to prrs virus (prrsv) infection is poorly understood. genome-wide association studies (gwas) were done here using data from 928 fetuses from pregnant gil ... | 2016 | 26846722 |
| no clear effect of initiating vaccination against common endemic infections on the amounts of prescribed antimicrobials for danish weaner and finishing pigs during 2007-2013. | it is often stated that vaccines may help reduce antimicrobial use in swine production. however, limited evidence is available outside clinical trials. we studied the change in amounts of antimicrobials prescribed for weaners and finishers in herds following initiation of vaccination against five common endemic infections: mycoplasma hyopneumoniae, actinobacillus pleuropneumoniae, porcine circovirus type ii, porcine reproductive and respiratory syndrome virus, and lawsonia intracellularis. compa ... | 2016 | 28138438 |
| enriched housing reduces disease susceptibility to co-infection with porcine reproductive and respiratory virus (prrsv) and actinobacillus pleuropneumoniae (a. pleuropneumoniae) in young pigs. | until today, anti-microbial drugs have been the therapy of choice to combat bacterial diseases. resistance against antibiotics is of growing concern in man and animals. stress, caused by demanding environmental conditions, can reduce immune protection in the host, influencing the onset and outcome of infectious diseases. therefore psychoneuro-immunological intervention may prove to be a successful approach to diminish the impact of diseases and antibiotics use. this study was designed to investi ... | 2016 | 27606818 |
| a survey of porcine respiratory disease complex (prdc) associated pathogens among commercial pig farms of korea via oral fluid method. | oral fluid analysis for monitoring herd is an interest in commercial pig farms in korea. the aim of this study was to investigate the positive rates and correlation of eight pathogens associated with porcine respiratory disease complex (prdc) by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. the samples collected by rope-chewing method were analyzed with reverse-transcription polymerase chain reaction (rt-pcr) or standard pcr depending on microorganisms. the pathogens ... | 2016 | 27586468 |
| factors associated with herd-level prrsv infection and age-time to seroconversion in farrow-to-finish herds. | factors associated with porcine reproductive and respiratory syndrome virus (prrsv) infection were investigated in 109 herds. serums from four batches of pigs (4, 10, 16 and 22 weeks, 15 pigs/batch) were tested by elisa for prrsv antibodies. infection by mycoplasma hyopneumoniae (mhp), actinobacillus pleuropneumoniae, h1n1 and h1n2 swine influenza a viruses (swiav) and pcv2 were detected by specific serological or pcr tests. data related to herd characteristics, biosecurity, management housing a ... | 2016 | 27527759 |
| simultaneous typing of seven porcine pathogens by multiplex pcr with a gexp analyser. | a novel high-throughput method was developed for simultaneous detection and differentiation of seven porcine pathogens by multiplex pcr based on a genomelab gene expression profiler (gexp) analyser. the pathogens included in this study were pseudorabies virus (prv), classical swine fever virus (csfv), african swine fever virus (asfv), porcine reproductive and respiratory syndrome virus (prrsv), porcine parvovirus (ppv), porcine circovirus type 2 (pcv-2) and japanese encephalitis virus (jev). sev ... | 2016 | 26706731 |
| insulated isothermal reverse transcriptase pcr (iirt-pcr) for rapid and sensitive detection of classical swine fever virus. | classical swine fever (csf) is an oie-listed disease that can have a severe impact on the swine industry. user-friendly, sensitive, rapid diagnostic tests that utilize low-cost field-deployable instruments for csf diagnosis can be useful for disease surveillance and outbreak monitoring. in this study, we describe validation of a new probe-based insulated isothermal reverse transcriptase pcr (iirt-pcr) assay for rapid detection of classical swine fever virus (csfv) on a compact, user-friendly dev ... | 2016 | 25644051 |
| pseudorabies virus and brucella abortus from an expanding wild pig ( sus scrofa ) population in southern oklahoma, usa. | wild pigs ( sus scrofa ) are causing increasing ecologic and economic damage at a global scale. because wild pigs can carry ≥65 diseases that affect livestock, their widespread expansion threatens native wildlife and livestock. we screened wild pigs from south-central oklahoma, us for antibodies against brucella abortus , pseudorabies virus (prv), and porcine reproductive and respiratory syndrome virus (prrs). these pathogens were chosen because they are part of eradication programs in the us an ... | 2016 | 27124329 |