Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| lambda interferon (ifn-lambda) in serum is decreased in hantavirus-infected patients, and in vitro-established infection is insensitive to treatment with all ifns and inhibits ifn-gamma-induced nitric oxide production. | hantaviruses, causing hemorrhagic fever with renal syndrome (hfrs) and hantavirus cardiopulmonary syndrome (hcps), are known to be sensitive to nitric oxide (no) and to pretreatment with type i and ii interferons (alpha interferon [ifn-alpha]/ifn-beta and ifn-gamma, respectively). elevated serum levels of no and ifn-gamma have been observed in hfrs patients, but little is known regarding the systemic levels of other ifns and the possible effects of hantaviruses on innate antiviral immune respons ... | 2007 | 17522204 |
| detection of dobrava hantaviruses in apodemus agrarius mice in the transdanubian region of hungary. | dobrava hantavirus (dobv) belongs to the genus hantavirus of the family bunyaviridae, and is carried by yellow necked and striped field mice (apodemus flavicollis and apodemus agrarius), respectively. the aim of this study was to detect and genetically characterize new dobv strains in rodents captured in the transdanubian region of hungary. rodent corpses were dissected and lung tissues were used for hantavirus detection by sybr green-based real-time rt-pcr using specific primers located in the ... | 2007 | 17524512 |
| nucleotide sequence characterization and phylogenetic analysis of hantaviruses isolated in shandong province, china. | china is the most severe endemic area of hemorrhagic fever with renal syndrome (hfrs) in the world with 30,000-50,000 cases reported annually, which accounts for more than 90% of total number of cases worldwide. the incidence rate of the syndrome in shandong province is one of the highest in china, which has ever reached 50 per 100,000 persons per year. however, the molecular characteristics of hantaviruses (hv) epidemic in shandong province remain unclear. therefore it is useful to clarify nucl ... | 2007 | 17531126 |
| puumala and tula hantaviruses in france. | the first genome sequences of tula (tulv) and puumala (puuv) hantaviruses undoubtedly originated from france were recovered from tissue samples of european common voles and bank voles captured in jura region. genetic analysis of s and m segments of french puuv strain revealed its highest similarity to strains from neighboring belgium and germany and also from slovakia. on phylogenetic trees, french puuv strain was placed within the central european lineage formed by strains from these three coun ... | 2007 | 17532080 |
| dynein-dependent transport of the hantaan virus nucleocapsid protein to the endoplasmic reticulum-golgi intermediate compartment. | in contrast to most negative-stranded rna viruses, hantaviruses and other viruses in the family bunyaviridae mature intracellularly, deriving the virion envelope from the endoplasmic reticulum (er) or golgi compartment. while it is generally accepted that old world hantaviruses assemble and bud into the golgi compartment, some studies with new world hantaviruses have raised the possibility of maturation at the plasma membrane as well. overall, the steps leading to virion assembly remain largely ... | 2007 | 17537852 |
| puumala hantavirus viremia diagnosed by real-time reverse transcriptase pcr using samples from patients with hemorrhagic fever and renal syndrome. | puumala virus (puuv) is the endemic hantavirus in northern sweden and causes nephropathia epidemica (ne), a milder form of hemorrhagic fever with renal syndrome. there is a need for fast and reliable diagnostics to differentiate the disease from other infections. by aligning virus rna sequences isolated from 11 different bank voles and one human patient, we designed a real-time reverse transcriptase (rt) pcr method for detection of puuv rna. the real-time rt-pcr assay showed linearity from 20 to ... | 2007 | 17537944 |
| [prevalence of antibodies to hantavirus among hemodialysis patients with end-stage renal failure in kaunas and its district]. | the objective of this study was to investigate the prevalence of antibodies to hantaviruses among hemodialysis patients with end-stage renal failure in kaunas and its district. | 2007 | 17551280 |
| hantavirus and arenavirus antibodies in persons with occupational rodent exposure. | rodents are the principal hosts of sin nombre virus, 4 other hantaviruses known to cause hantavirus pulmonary syndrome in north america, and the 3 north american arenaviruses. serum samples from 757 persons who had worked with rodents in north america and handled neotomine or sigmodontine rodents were tested for antibodies against sin nombre virus, whitewater arroyo virus, guanarito virus, and lymphocytic choriomeningitis virus. antibodies against sin nombre virus were found in 4 persons, agains ... | 2007 | 17553266 |
| novel hantavirus sequences in shrew, guinea. | 2007 | 17554814 | |
| update on hantavirus in oklahoma: are we missing cases? | hantavirus pulmonary syndrome (hps) was first recognized in 1993. through july 6, 2005, 396 cases have been reported in the us, including 50 from kansas, texas, and oklahoma. we report the second case of hps in oklahoma and present data from rodent testing to support the presence of hantaviruses across oklahoma. we examined oklahoma death certificates for 1999-2003 for possible unreported hps cases. the rate of death in young adults 15-49 years due to acute respiratory distress syndrome was 69% ... | 2007 | 17557601 |
| [an epidemiologle investigation of hantaviruses carried by rodent hosts in hunan province]. | to explore the hantavirus infection and their genotype in rodents in hunan. | 2007 | 17575936 |
| envelope glycoproteins of hantavirus can mediate cell-cell fusion independently. | hantaviruses (htvs) are enveloped viruses and can induce low ph-dependent cell fusion. in this report we molecularly cloned viral glycoproteins (gps) cdna and nucleocapsid (np) cdna of two strains of hantaan virus and one strain of seoul virus and expressed in vero e6 cells under control of a cmv promoter. the examinations of viral gene expressions were carried out by ifa and immune-precipitation. after treatment with low ph (ph 5.8) medium the syncytium were observed in the cells transfected wi ... | 2007 | 17619252 |
| a new hantaan-like virus in rodents (apodemus peninsulae) from northeastern china. | lung tissue samples of 76 korean field mice (apodemus peninsulae) collected from northeastern china bordering with far east russia and korea were detected for hantavirus partial m-segment or entire s-segment sequences by rt-pcr and 481-nt mitochondrial dna fragment of the rodents. four a. peninsulae mice were found positive for partial m-segment of hantavirus. sequence analyses of partial m-segment or/and entire s-segment of the hantaviruses revealed that three were closely related to hantaan vi ... | 2007 | 17624464 |
| hantavirus disease outbreak in germany: limitations of routine serological diagnostics and clustering of virus sequences of human and rodent origin. | in europe, hemorrhagic fever with renal syndrome results mainly from infection with puumala virus (puuv) or dobrava virus. for 31 patients from a hantavirus disease outbreak in lower bavaria, a district in southeast germany, serodiagnosis was undertaken by enzyme-linked immunosorbent assay, immunofluorescence assay, and immunoblot analysis. in a few of these cases, however, puuv-specific typing of antibodies by these standard assays failed and a virus neutralization assay under biosafety level 3 ... | 2007 | 17626170 |
| a longitudinal study of hantavirus infection in three sympatric reservoir species in agroecosystems on the argentine pampa. | prevalence of antibody reactive with sin nombre hantavirus (snv) was evaluated from rodents captured over 31 months (march 1988 to september 1990) from six mark-recapture grids on the central argentine pampa. the most frequently infected rodents were: akodon azarae (31/459), necromys benefactus (8/141), and oligoryzomys flavescens (10/281), which are known hosts of pergamino, maciel, and lechiguanas hantaviruses, respectively. relative population density and antibody prevalence varied seasonally ... | 2007 | 17627443 |
| early innate immune responses to sin nombre hantavirus occur independently of ifn regulatory factor 3, characterized pattern recognition receptors, and viral entry. | sin nombre virus (snv) is a highly pathogenic new world virus and etiologic agent of hantavirus cardiopulmonary syndrome. we have previously shown that replication-defective virus particles are able to induce a strong ifn-stimulated gene (isg) response in human primary cells. rna viruses often stimulate the innate immune response by interactions between viral nucleic acids, acting as a pathogen-associated molecular pattern, and cellular pattern-recognition receptors (prrs). ligand binding to prr ... | 2007 | 17641046 |
| human cd8+ t cell memory generation in puumala hantavirus infection occurs after the acute phase and is associated with boosting of ebv-specific cd8+ memory t cells. | the induction and maintenance of t cell memory is incompletely understood, especially in humans. we have studied the t cell response and the generation of memory during acute infection by the puumala virus (puuv), a hantavirus endemic to europe. it causes a self-limiting infection with no viral persistence, manifesting as hemorrhagic fever with renal syndrome. hla tetramer staining of pbmc showed that the cd8(+) t cell response peaked at the onset of the clinical disease and decreased within the ... | 2007 | 17641066 |
| [detection of igm antibody against hantavirus by chemiluminescent enzyme-linked immunosorbent assay]. | to develop a chemiluminescent enzyme-linked immunosorbent assay (cleia) for the detection of htnv igm antibody. | 2007 | 17653327 |
| [hantavirus cardiopulmonary syndrome in the triângulo mineiro and alto paranaíba regions, state of minas gerais, 1998-2005: clinical-epidemiological aspects of 23 cases]. | the epidemiological, clinical, laboratory and treatment findings from 23 cases of hantavirus cardiopulmonary syndrome were analyzed. these cases were identified either serologically or immunohistochemically in hospitals in the municipality of uberlândia, state of minas gerais. fever (100%), dyspnea (100%) and myalgia (78%) were the symptoms most frequently observed in this sample. the most prevalent physical signs were hypotension (65%) and tachycardia (65%). the most common laboratory findings ... | 2007 | 17653464 |
| hantavirus infection in east asia. | hantaviruses are enveloped rna viruses that belong to the hantavirus genus of the family bunyaviridae. these viruses persistently infect their rodent reservoirs without causing disease. the virus is transmitted to humans via the inhalation of infectious aerosols generated from contaminated animal secretions or through the contaminated saliva of animal bites. hantaviruses cause haemorrhagic fever with renal syndrome in euro-asia, and hantavirus pulmonary syndrome (hps) in north and south america. ... | 2007 | 17655929 |
| the cytoplasmic tails of uukuniemi virus (bunyaviridae) g(n) and g(c) glycoproteins are important for intracellular targeting and the budding of virus-like particles. | functional motifs within the cytoplasmic tails of the two glycoproteins g(n) and g(c) of uukuniemi virus (uuk) (bunyaviridae family) were identified with the help of our recently developed virus-like particle (vlp) system for uuk virus (a. k. overby, v. popov, e. p. neve, and r. f. pettersson, j. virol. 80:10428-10435, 2006). we previously reported that information necessary for the packaging of ribonucleoproteins into vlps is located within the g(n) cytoplasmic tail (a. k. overby, r. f. petters ... | 2007 | 17670814 |
| [transmission by feces of infected rodents. hantavirus illnesses are increasing]. | 2007 | 17672003 | |
| [the evaluation of viral load in patients with hemorrhagic fever with renal syndrome (hfrs) by real-time polymerase chain reaction]. | the system for real-time polymerase chain reaction was developed for detection of puumala virus rnaand quantitative analysis of viral rnaload in clinical samples. dynamics of viral load in blood samples from 12 patients with hfrs (from day 4 to day 17 of the disease) was studied. at day 4 viral rna load varied from 4 x 10(4) to 1 x 10(6) copies/ml. during the period of observation viral load decreased by 10 - 200 times and was not detected in majority of patients to day 11 - 15 of the disease. t ... | 2007 | 17672134 |
| seminiferous epithelium cycle of a hantavirus reservoir, the long-tailed mouse oligoryzomys flavescens (rodentia-cricetidae). | oligoryzomys flavescens, a common rodent of the temperate regions of south america, has been identified as a hantavirus reservoir. there is still little information concerning its reproductive biology, which is essential to devise effective control measurement of natural populations. this rodent is a seasonal breeder and adult males exhibit a short period of testicular regression during winter months (june-august). in the present study we provided a histological and ultrastructural analysis of t ... | 2007 | 17675200 |
| prevalence of anti-hantavirus antibodies in patients with hypertransaminemia in madrid (spain). | the hantaviruses are involved in a number of clinical syndromes of different severity and prognosis. hantaviruses are widely distributed around the world, but the spectrum of illnesses they cause outside recognized endemic areas is unclear. a retrospective analysis was performed to detect anti-hantavirus antibodies in the serum of patients with hypertransaminemia of unknown etiology and in that of healthy members of the general population of madrid (spain). antibodies were detected by indirect i ... | 2007 | 17690415 |
| seroepidemiological survey of rodents collected at a u.s. military installation, yongsan garrison, seoul, republic of korea. | a seroepidemiological study of selected rodent-borne diseases (hantavirus [seoul [seo] virus], scrub typhus [orientia tsutsugamushi], murine typhus [rickettsia typhi], and leptospirosis [leptospira interrogans]), as part of the u.s. military rodent surveillance and control program, was conducted from 2001 through 2005 at yongsan garrison, seoul, republic of korea. rodents were collected to determine the prevalence of rodent-borne diseases at a u.s. military installation in an urban environment. ... | 2007 | 17691691 |
| acute hantavirus infection or renal transplant rejection. | hantaviruses belong to the so-called emerging pathogens that are transmitted to humans by infected rodents and their excreta. in central europe, hantavirus infections usually occur in a mild to moderate form of hemorrhagic fever with renal syndrome. in contrast to the mostly benign or even asymptomatic course of hantavirus infections in previously healthy individuals, the acute hantavirus infection in kidney transplant recipients represents an exceptional situation regarding diagnosis and therap ... | 2007 | 17692069 |
| andes and prospect hill hantaviruses differ in early induction of interferon although both can downregulate interferon signaling. | hantavirus pulmonary syndrome (hps) is a severe respiratory disease which is thought to result from a dysregulated immune response to infection with pathogenic hantaviruses, such as sin nombre virus or andes virus (andv). other new world hantaviruses, such as prospect hill virus (phv), have not been associated with human disease. activation of an antiviral state and cell signaling in response to hantavirus infection were examined using human primary lung endothelial cells, the main target cell i ... | 2007 | 17202220 |
| case report of hantavirus pulmonary syndrome and review. | a previously healthy 29-year-old male presented to the emergency department with pleuritic chest pain, nonproductive cough, and dyspnea. he was treated empirically for community acquired pneumonia and discharged to home. two days later, the patient presented with respiratory distress along with neutrophilia, thrombocytopenia, elevated liver function tests, and hemoconcentration. radiographs of his chest showed bilateral lung infiltrates and pleural effusions. he was admitted to the hospital and ... | 2007 | 17694939 |
| ocular features of hantavirus infection. | hantavirus infections are an emerging infectious disease that is beginning to be recognized both worldwide and in india as a cause of hemorrhagic fever that may present as a pulmonary syndrome or as a renal syndrome. reports of ocular involvement are rare and include transient myopia, low intraocular pressure, conjunctival hemorrhages and changes of intraocular dimensions. eleven patients (10 males, one female, mean age 37.6 years) were admitted to the intensive care unit for pyrexia of unknown ... | 2007 | 17699950 |
| brush mouse (peromyscus boylii) population dynamics and hantavirus infection during a warm, drought period in southern arizona. | we monitored limestone canyon hantavirus (lscv) antibody prevalence, host (brush mouse, peromyscus boylii) abundance, and environmental variables (temperature and rainfall) in brush mice captured on three trapping webs in southern arizona for 5 yr. although seasonal patterns were subtle, we observed large multiyear variation in population abundance and antibody prevalence. limestone canyon hantavirus infection in brush mouse populations varied over time with prevalence ranging from 0% to 33%. at ... | 2007 | 17984263 |
| cluster of cases of hantavirus pulmonary syndrome in alberta, canada. | in may 2005, a cluster of four hantavirus pulmonary syndrome (hps) cases was confirmed in alberta, canada. the cluster is unusual given that three cases were from a single family and involved a 7-year-old child. this is the first family cluster reported in canada and includes one of the youngest cases of hps reported in north america. | 2007 | 17984353 |
| seewis virus, a genetically distinct hantavirus in the eurasian common shrew (sorex araneus). | more than 20 years ago, hantaviral antigens were reported in tissues of the eurasian common shrew (sorex araneus), eurasian water shrew (neomys fodiens) and common mole (talpa europea), suggesting that insectivores, or soricomorphs, might serve as reservoirs of unique hantaviruses. using rt-pcr, sequences of a genetically distinct hantavirus, designated seewis virus (swsv), were amplified from lung tissue of a eurasian common shrew, captured in october 2006 in graubünden, switzerland. pair-wise ... | 2007 | 17967200 |
| [genetic susceptibility to andes hantavirus: association between severity of disease and hla alíeles in chilean patients]. | andes hantavirus (andv) infection in chile has a variable clinical expression, and infected individuals may present with different grades of disease severity. this study aimed to determine if clinical expression of andv infection in chilean patients is associated with the hla system. hla alíeles a, b, drb1 and dqb1, were studied in two groups of patients with confirmed andv infection: 41 patients with a mild disease course (without respiratory failure and cardiovascular shock) and 46 patients wi ... | 2007 | 17989838 |
| [hantavirus specific igg antibodies in population of zoologists and forest workers]. | serological diagnostic tests were introduced for detection of immunoresponse against to etiological agents of current infectious diseases. interpretation criteria for those tests should eliminate unspecific reactions, cross-reactivity with related viruses as well as traces amounts of immunological response against to the past infections. in the case of past hantaviruses infections for obtaining an adequate seroepidemiological data revalidation of this tests are required. in present studies puuma ... | 2007 | 18069384 |
| [the cross and unspecific reactions in serological examination for antibodies against hantavirus puumala]. | a serological survey of 78 zoologist capturing small wild rodents in their environment, 9 patients with suspected hantavirus infections and 21 patients with acute renal dysfunction for antibodies to hantaviruses was conducted in poland. survey was done by the indirect elisa with puumala and hantaan virus antigens. out of the 78 mammalogists 15 were seropositive for hantavirus puumala igg without history of clinical illness. analysis of relation between reactive zoologist's sera igg with antigens ... | 2007 | 18069385 |
| [occurrence of human hantavirus infections in poland]. | a spread of hantavirus infections causing hemorrhagic fever with renal syndrome is one of the rising epidemiological problems in the world. there is evidence that this type of viruses exists in almost all european countries. it is known that in poland all the species of wild rodents hosting european hantaviruses are present. however, the data on hantavirus are very limited and information on incidence and prevalence of hantavirus infections among humans and animals in our country is lacking. up ... | 2007 | 18069386 |
| [study on the recombinant expression of hantaan virus protein n and the establishment and application of rnp-igm direct capture elisa]. | to clone the gene encoding nucleocapsid protein (np) of hantavirus strain z10 (hv-z10), to construct its prokaryotic expression system as well as to establish a rnp-igm direct capture elisa based on hrp-labeled recombinant np (rnp), in order to detect serum samples of patients suffering from hemorrhagic fever with renal syndrome (hfrs) and to evaluate the effects of detection. | 2007 | 18069562 |
| tula and puumala hantavirus nss orfs are functional and the products inhibit activation of the interferon-beta promoter. | the s rna genome segment of hantaviruses carried by arvicolinae and sigmodontinae rodents encodes the nucleocapsid (n) protein and has an overlapping (+1) open reading frame (orf) for a putative nonstructural protein (nss). the aim of this study was to determine whether the orf is functional. a protein corresponding to the predicted size of tula virus (tulv) nss was detected using coupled in vitro transcription and translation from a cloned s segment cdna, and a protein corresponding to the pred ... | 2007 | 17705180 |
| thottapalayam virus is genetically distant to the rodent-borne hantaviruses, consistent with its isolation from the asian house shrew (suncus murinus). | thottapalayam (tpm) virus belongs to the genus hantavirus, family bunyaviridae. the genomes of hantaviruses consist of three negative-stranded rna segments (s, m and l) encoding the virus nucleocapsid (n), glycoprotein (gn, gc), and polymerase (l) proteins, respectively. the genus hantavirus contains predominantly rodent-borne viruses, with the prominent exception of tpm virus which was isolated in india in 1964 from an insectivore, suncus murinus, commonly referred to as the asian house shrew o ... | 2007 | 17711577 |
| detection and typing of human pathogenic hantaviruses by real-time reverse transcription-pcr and pyrosequencing. | because the clinical course of human infections with hantaviruses can vary from subclinical to fatal, rapid and reliable detection of hantaviruses is essential. to date, the diagnosis of hantavirus infection is based mainly on serologic assays, and the detection of hantaviral rna by the commonly used reverse transcription (rt)-pcr is difficult because of high sequence diversity of hantaviruses and low viral loads in clinical specimens. | 2007 | 17717126 |
| differential resource allocation in deer mice exposed to sin nombre virus. | the resource allocation hypothesis predicts that reproductive activity suppresses immunocompetence; however, this has never been tested in an endemic disease system with free-ranging mammals. we tested the resource allocation hypothesis in wild deer mice (peromyscus maniculatus) with natural exposure to sin nombre virus (snv). immunocompetence was estimated from the extent of swelling elicited after deer mice were injected with phytohemagglutinin (pha); swelling is positively correlated with imm ... | 2007 | 17717814 |
| elevated testosterone and reduced 5-hiaa concentrations are associated with wounding and hantavirus infection in male norway rats. | among rodents that carry hantaviruses, males are more likely to engage in aggression and to be infected than females. one mode of hantavirus transmission is via the passage of virus in saliva during wounding. the extent to which hantaviruses cause physiological changes in their rodent host that increase aggression and, therefore, virus transmission has not been fully documented. to assess whether steroid hormones and neurotransmitters contribute to the correlation between aggression and seoul vi ... | 2007 | 17719050 |
| delayed density-dependent prevalence of sin nombre virus antibody in montana deer mice (peromyscus maniculatus) and implications for human disease risk. | american hantaviruses cause a severe respiratory disease known as hantavirus pulmonary syndrome (hps). in the united states, sin nombre virus (snv), carried by the deer mouse ( peromyscus maniculatus), is the etiologic agent in the majority of hps cases. the relationship between deer mouse population density and snv infection prevalence in deer mice is poorly understood. our purpose was to clarify this relationship by demonstrating the existence of delayed-density-dependent prevalence of snv inf ... | 2007 | 17767405 |
| bilateral acute acquired toxoplasmic retinochoroiditis after steroid therapy for hantavirus pulmonary syndrome: case report. | description of a case of acute acquired ocular toxoplasmosis following hantavirus pulmonary syndrome. a 41-year-old man presenting hantavirus pulmonary syndrome, confirmed in the laboratory by detection of igm antibodies to the virus, was submitted to high doses of intravenous corticosteroids for two months. after clinical improvement of hantavirus pulmonary syndrome the patient presented visual loss in both eyes that was secondary to a toxoplasmosis retinitis. the retinitis resolved with anti-t ... | 2007 | 17768562 |
| endemic hantavirus infection impairs the winter survival of its rodent host. | the influence of pathogens on host fitness is one of the key questions in infection ecology. hantaviruses have coevolved with their hosts and are generally thought to have little or no effect on host survival or reproduction. we examined the effect of puumala virus (puuv) infection on the winter survival of bank voles (myodes glareolus), the host of this virus. the data were collected by monitoring 22 islands over three consecutive winters (a total of 55 island populations) in an endemic area of ... | 2007 | 17824420 |
| emergence and persistence of hantaviruses. | hantaviral diseases have been recognized for hundreds of years but, until 1976, they had not been associated with an infectious agent. when lee and colleagues isolated what is now known as hantaan virus, the techniques they introduced allowed further investigations into the etiology of the classical hantavirus disease, hemorrhagic fever with renal syndrome (hfrs), now known to be caused by any of multiple hantaviruses. the discovery of hantavirus pulmonary syndrome (hps) in the new world, and th ... | 2007 | 17848067 |
| first detection of dobrava hantavirus from a patient with severe haemorrhagic fever with renal syndrome by sybr green-based real time rt-pcr. | dobrava hantavirus (dobv) infection was diagnosed in a previously healthy 46-y-old hunter suffering from severe haemorrhagic fever with renal syndrome (hfrs). specific igm antibodies against dobv were identified by an immunofluorescence assay, while viral nucleic acid was detected by the molecular method, confirming the diagnosis. our results reveal an existing risk of dobv transmission to humans in hungary. | 2007 | 17852891 |
| soochong virus and amur virus might be the same entities of hantavirus. | amur virus (amrv) and soochong virus (soov) were reported to be carried by korean field mice (apodemus peninsulae) in the far east of russia, china, and korea. the distinction and demarcation between these two viruses have been a matter of debate. in order to clarify this issue and to confirm taxonomic position of amrv and soov, the extensive phylogenetic analyses based on entire s segment and entire m segment sequences of amrv, soov and other reference virus strains deposited in genbank, were c ... | 2007 | 17854038 |
| genetic evidence of puumala and tula hantaviruses in rodents in the jura region, france--preliminary results. | 2007 | 17868570 | |
| significant increase of hantavirus infections in germany since the beginning of 2007. | 2007 | 17868605 | |
| regulatory t cell-like responses in deer mice persistently infected with sin nombre virus. | hantavirus cardiopulmonary syndrome is a zoonotic illness associated with a systemic inflammatory immune response, capillary leak, noncardiogenic pulmonary edema, and shock in humans. cytokines, including tnf, ifn-gamma, and lymphotoxin, are thought to contribute to its pathogenesis. in contrast, infected rodent reservoirs of hantaviruses experience few or no pathologic changes and the host rodent can remain persistently infected for life. generally, it is unknown why such dichotomous immune res ... | 2007 | 17875986 |
| [identification of amur like virus in apodemus peninsulae and its molecular characteristics in china]. | to confirm the existence of amur-like viruses in apodemus peninsulae in china, and to understand the molecular characteristics of these viruses. | 2007 | 17877180 |
| regulatory t cells enhance persistence of the zoonotic pathogen seoul virus in its reservoir host. | hantaviruses are zoonotic pathogens that maintain a persistent infection in their reservoir hosts, yet the mechanisms mediating persistence remain unknown. regulatory t cell responses cause persistent infection by suppressing proinflammatory and effector t cell activity; hantaviruses may exploit these responses to cause persistence. to test this hypothesis, male norway rats were inoculated with seoul virus and regulatory t cells were monitored during infection. increased numbers of cd4(+)cd25(+) ... | 2007 | 17878294 |
| [identification of a new puumala hantavirus subtype in rodents from china]. | in order to detect the nucleic acid of puumala hantavirus, rna was extracted from lungs of bank voles captured in northeast china, and partial s and m genome segments of puumala virus were amplified by rt-pcr and sequenced. phylogenetic analysis suggested that chinese puumala virus had diverged from the common node of puuv, with accumulating nucleotide substitutions and formed a distinct lineage from other puumala viruses. newly found puumala virus was most closely related to the kamiiso-8cr-95 ... | 2007 | 17894236 |
| immunopathogenesis of hantavirus pulmonary syndrome and hemorrhagic fever with renal syndrome: do cd8+ t cells trigger capillary leakage in viral hemorrhagic fevers? | there are many viruses known to cause viral hemorrhagic fevers in humans. the mechanisms causing hemorrhage are likely to vary among viruses. some viruses, such as marburg virus, are directly cytopathic to infected endothelial cells, suggesting infection of endothelial cells alone can cause hemorrhage. on the other hand, there are viruses which infect endothelial cells without causing any cytopathic effects, suggesting the involvement of host immune responses in developing hemorrhage. typical ex ... | 2007 | 17897725 |
| use of saccharomyces cerevisiae-expressed recombinant nucleocapsid protein to detect hantaan virus-specific immunoglobulin g (igg) and igm in oral fluid. | hantaan virus is the causative agent of severe hemorrhagic fever with renal syndrome. clinical surveillance for hantaan virus infection is unreliable, and laboratory verification is essential. the detection of virus-specific immunoglobulin m (igm) and igg in serum is most commonly used for the diagnosis of hantavirus infection. testing of oral fluid samples instead of serum offers many advantages for surveillance. however, commercial tests for hantavirus-specific antibodies are unavailable. for ... | 2007 | 17913864 |
| case report: severe neurological manifestation of dobrava hantavirus infection. | in europe infection with puumala or dobrava viruses causes hemorrhagic fever with renal syndrome (hfrs). in the course of hfrs, mild neurological symptoms such as headache, vertigo, and nausea are common. however, the data about the occurrence of severe, potentially life-threatening neurological manifestations are rather scarce. here, we present a case of hfrs with serologically proven dobrava virus infection complicated by epileptic seizures and hemiparesis due to focal encephalitis. | 2007 | 17935168 |
| muju virus, a novel hantavirus harboured by the arvicolid rodent myodes regulus in korea. | acute-phase sera from >5 % of cases of haemorrhagic fever with renal syndrome occurring annually in korea have been found to exhibit a fourfold or higher antibody titre to puumala virus (puuv) than to hantaan virus (htnv) by double-sandwich igm elisa, suggesting the existence of a puuv-related hantavirus. based on the phylogenetic relationships among arvicolid rodents, the royal vole (myodes regulus) was targeted as a likely reservoir host of hantavirus. using rt-pcr, a genetically distinct hant ... | 2007 | 17947538 |
| pathogenic hantaviruses, northeastern argentina and eastern paraguay. | we describe the first, to our knowledge, cases of han-tavirus pulmonary syndrome in northeastern argentina and eastern paraguay. andes and juquitiba (juq) viruses were characterized. juqv was also confirmed in 5 oligoryzomys nigripes reservoir species from misiones. a novel akodon-borne genetic hantavirus lineage was detected in 1 rodent from the biologic reserve of limoy. | 2007 | 17953094 |
| morphological characterization of hantavirus hv114 by electron microscopy. | this study was sought to investigate the propagation and morphogenesis of a new strain of hantavirus, hv114. | 2007 | 17259735 |
| the expression and genetic immunization of chimeric fragment of hantaan virus m and s segments. | hemorrhagic fever with renal syndrome (hfrs), which is characterized by severe symptoms and high mortality, is caused by hantavirus. there are still no effective prophylactic vaccines directed to hfrs until now. in this research, we fused expressed g2 fragment of m segment and 0.7kb fragment of s segment. we expect it could be a candidate vaccine. chimeric gene g2s0.7 was first expressed in prokaryotic expression system pgex-4t. after inducing expressed fusion proteins, gst-g2s0.7 was induced an ... | 2007 | 17266922 |
| hantavirus pulmonary syndrome: high-resolution ct findings in one patient. | physical examination demonstrated petechiae, leg oedema and mild dyspnoea. chest radiograph showed minimal bilateral hazy increased opacification, mainly on the right side, and small bilateral pleural effusions. high-resolution ct demonstrated extensive bilateral ground-glass opacities most severe in the middle and lower lung zones. also noted were a few slightly thickened interlobular septa, a few poorly defined small nodules, bronchial wall thickening and small bilateral pleural effusions. blo ... | 2007 | 17267465 |
| degrons at the c terminus of the pathogenic but not the nonpathogenic hantavirus g1 tail direct proteasomal degradation. | pathogenic hantaviruses cause two human diseases: hantavirus pulmonary syndrome (hps) and hemorrhagic fever with renal syndrome (hfrs). the hantavirus g1 protein contains a long, 142-amino-acid cytoplasmic tail, which in ny-1 virus (ny-1v) is ubiquitinated and proteasomally degraded (e. geimonen, i. fernandez, i. n. gavrilovskaya, and e. r. mackow, j. virol. 77: 10760-10768, 2003). here we report that the g1 cytoplasmic tails of pathogenic andes (hps) and hantaan (hfrs) viruses are also degraded ... | 2007 | 17267477 |
| seasonal variation of north american form of gigantolaelaps mattogrossensis (acari: laelapidae) on marsh rice rat in southern coastal texas. | the ectoparasites of a small mammal community within an intertidal zone in the upper gulf coast region of texas were studied to assess the seasonal variation in abundances of the mite gigantolaelaps mattogrossensis (fonseca) (acari: laelapidae) on the marsh rice rat, oryzomys palustris (harlan). further study into the ecology and dynamics of this parasite-host relationship was deemed to be necessary to expand the understanding of these potential participants in the ecology of bayou hantavirus, a ... | 2007 | 17294924 |
| cross-sectional survey on hantavirus seroprevalence in canton st. gallen, switzerland. | in 2002 the first endemic hantavirus infection in switzerland was detected only by chance following a broad spectrum of diagnostics. this raised the question, whether hantavirus infection should be included in the differential diagnosis of febrile illness of patients in switzerland. in order to estimate the frequency of hantavirus infections in switzerland, this survey on hantaviral seroprevalence was conducted in the canton st. gallen. | 2007 | 17299664 |
| evaluation of the efficacy of disinfectants against puumala hantavirus by real-time rt-pcr. | puumala virus, a hantavirus belonging to the bunyaviridae family, causes a human disease known as nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome. the implementation of effective decontamination procedures is critical in hantavirus research to minimize the risk of personnel exposure. this study investigated the efficacy of clidox((r)), dettol((r)), ethanol, halamid-d((r)), peracetic acid, sodium hypochloride and virkon((r))s for inactivating puumala virus. a real-tim ... | 2007 | 17188760 |
| development of serological assays for thottapalayam virus, an insectivore-borne hantavirus. | thottapalayam virus (tpmv), a member of the genus hantavirus in the family bunyaviridae, was isolated from an insectivore, suncus murinus (musk shrew), captured in southern india in 1964. while the isolation of tpmv predates the discovery of the prototype hantaan virus, little is known about its genetics and biology. to date, preliminary evidence suggests that tpmv differs significantly, both antigenically and genetically, from all known rodent-borne hantaviruses. however, since detailed epizoot ... | 2007 | 17182762 |
| radiology of biological weapons--old and the new? | 2007 | 17174174 | |
| emerging and re-emerging viral infections in europe. | emerging viral infections are becoming a serious problem in europe in the recent years. this is particularly true for severe acute respiratory syndrome (sars), west nile virus (wnv) disease, toscana virus (tosv) disease, and potentially for avian influenza virus (h5n1). in contrast, emergence or re-emergence of severe viral infections, including tick borne encephalitis virus, and viral haemorrhagic fever caused by hantavirus and dengue virus have been frequently reported in several european coun ... | 2007 | 16944546 |
| seaport as a source of hantavirus: a study on isolated isles. | since small mammals from seaports have relative higher seroprevalences of hantavirus, this seroepidemiologic study was conducted on the isles in kinmen and lienchiang counties along the coast of southern mainland china to determine whether seaport may play the role as a source of hantavirus. among six species of small mammals trapped in kinmen county, only male mus musculus (6.7%) and rattus losea (5.3%) were found to be positive. in lienchiang county, five species of small mammals were trapped ... | 2007 | 17365077 |
| clusters of hantavirus infection, southern argentina. | person-to-person transmission of a hantavirus was first confirmed during a 1996 outbreak of hantavirus pulmonary syndrome in southern argentina, where andes virus is endemic. to identify other episodes of secondary transmission, we reviewed reports of 51 cases of hantavirus infection from this region (november 1993-june 2005). nine clusters involving 20 cases (39.2%) were found. two patients, who had symptoms 3 weeks after they shared risks for rodent exposure, were considered a cluster. the oth ... | 2007 | 17370522 |
| isolation and characterization of hantavirus carried by apodemus peninsulae in jilin, china. | to provide a better understanding of hantavirus epidemiology in china, korean field mice (apodemus peninsulae) and striped field mice (apodemus agrarius) were captured in jilin province, china, where haemorrhagic fever with renal syndrome (hfrs) is endemic. hantavirus antigens were detected in eight of the 130 a. peninsulae individuals and in four of the 193 a. agrarius individuals by using an immunofluorescence assay. partial s and m segments were amplified from all of the antigen-positive samp ... | 2007 | 17374775 |
| hantavirus infection in brazil: development and evaluation of an enzyme immunoassay and immunoblotting based on n recombinant protein. | the symptoms of hantavirus pulmonary syndrome may resemble those of other febrile illnesses. the development of an accurate diagnostic test should therefore improve clinical prognosis and be useful in epidemiologic studies. we evaluated the use of a recombinant antigen (rndelta(85)) based on the s-segment sequences of a brazilian hantavirus for detecting immunoglobulin m (igm) and immunoglobulin g (igg) antibodies against hantavirus in an indirect enzyme immunoassay (eia). we assayed 613 serum s ... | 2007 | 17383845 |
| index of suspicion. | 2007 | 17400825 | |
| demographic factors associated with prevalence of antibody to sin nombre virus in deer mice in the western united states. | we used long-term data collected for up to 10 yr (1994-2004) at 23 trapping arrays (i.e., webs and grids) in arizona, colorado, montana, and new mexico to examine demographic factors known or suspected to be associated with risk of infection with sin nombre virus (snv) in its natural host, the deer mouse (peromyscus maniculatus). gender, age (mass), wounds or scars, season, and local relative population densities were statistically associated with the period prevalence of antibody (used as a mar ... | 2007 | 17347388 |
| sin nombre virus infection of deer mice in montana: characteristics of newly infected mice, incidence, and temporal pattern of infection. | sin nombre virus (snv), hosted by the deer mouse (peromyscus maniculatus), is the principal cause of hantavirus pulmonary syndrome (hps) in north america. to improve our understanding of factors that contribute to the occurrence of hps, we conducted an extensive field study of the characteristics of newly infected (as determined by recent acquisition of antibody) deer mice and the temporal pattern of antibody acquisition (seroconversion) from 1994 through 2004 in montana, usa. we sampled 6,584 i ... | 2007 | 17347389 |
| two cases of hantavirus pulmonary syndrome in randolph county, west virginia: a coincidence of time and place? | hantavirus pulmonary syndrome (hps) is caused by an infection with viruses of the genus hantavirus in the western hemisphere. rodent hosts of hantaviruses are present throughout the united states. in july 2004, two hps case-patients were identified in randolph county, wv: a wildlife science graduate student working locally and a randolph county resident. we interviewed family members and colleagues, reviewed medical records, and conducted environmental studies at likely exposure sites. small mam ... | 2007 | 17360864 |
| detection of circulating antigen with a mab-based sandwich-elisa and its comparison with specific igm detection in sera of patients with hemorrhagic fever with renal syndrome. | a monoclonal antibody (1h2) against nucleocapsid protein of hantavirus was developed. a sandwich enzyme-linked immunosorbent assay (elisa) with the monoclonal antibody (mab) was established and evaluated for detecting circulating antigen (cag) in serum of patients with hemorrhagic fever with renal syndrome (hfrs). results were compared to that of an immunoglobulin m (igm)-detecting elisa. of 143 patients with hfrs, 106 were positive for cag of hantavirus and 128 positive for specific igm. among ... | 2007 | 17316085 |
| selection and characterization of scfv antibodies against the sin nombre hantavirus nucleocapsid protein. | rodent-borne hantaviruses cause hemorrhagic fever with renal syndrome (hfrs) in the old world and hantavirus cardio-pulmonary syndrome (hcps) in the new. most cases of hcps in north america are caused by sin nombre virus (snv). current viral detection technologies depend upon the identification of anti-viral antibodies in patient serum. detection of viral antigen may facilitate earlier detection of the pathogen. we describe here the characterization of two single-chain fv antibodies (scfvs), sel ... | 2007 | 17336997 |
| ex vivo stability of the rodent-borne hantaan virus in comparison to that of arthropod-borne members of the bunyaviridae family. | the possible effect of virus adaptation to different transmission routes on virus stability in the environment is not well known. in this study we have compared the stabilities of three viruses within the bunyaviridae family: the rodent-borne hantavirus hantaan virus (htnv), the sand fly-borne phlebovirus sandfly fever sicilian virus (sfsv), and the tick-borne nairovirus crimean-congo hemorrhagic fever virus (cchfv). these viruses differ in their transmission routes: sfsv and cchfv are vector bo ... | 2007 | 17337567 |
| the coiled-coil domain structure of the sin nombre virus nucleocapsid protein. | hantaviruses can cause hemorrhagic fever with a renal syndrome and hantavirus pulmonary syndrome when transmitted to humans. the nucleocapsid protein of hantaviruses encapsidates viral genomic rna and associates with transcription and replication complexes. both the amino and carboxy termini of the nucleocapsid protein had been predicted to form trimers prior to the formation of the ribonucleoprotein. crystal structures of amino-terminal fragments of the nucleocapsid protein showed the formation ... | 2007 | 17222867 |
| computer-aided diagnosis with potential application to rapid detection of disease outbreaks. | our objectives are to quickly interpret symptoms of emergency patients to identify likely syndromes and to improve population-wide disease outbreak detection. we constructed a database of 248 syndromes, each syndrome having an estimated probability of producing any of 85 symptoms, with some two-way, three-way, and five-way probabilities reflecting correlations among symptoms. using these multi-way probabilities in conjunction with an iterative proportional fitting algorithm allows estimation of ... | 2007 | 17225213 |
| utilization of hantavirus antibody results generated over a five-year period to develop an improved serologic algorithm for detecting acute sin nombre hantavirus infection. | in the united states most cases of hantavirus pulmonary syndrome (hps) are caused by the sin nombre virus (snv) and are typically identified by serology. the goal of this study was to assess the performance of our hantavirus serologic testing algorithm by reviewing results generated over five years. sera were screened for pan-hantavirus immunoglobulin (ig)g and igm by enzyme-linked immunosorbent assay (elisa). screen igg+ sera were then tested by immunoblot for snv glycoprotein-specific igg, and ... | 2007 | 17245757 |
| ribavirin, human convalescent plasma and anti-beta3 integrin antibody inhibit infection by sin nombre virus in the deer mouse model. | the new world hantavirus sin nombre virus (snv) is an aetiological agent for the often-fatal hantavirus cardiopulmonary syndrome (hcps). there is no disease model for snv and specific treatments for hcps do not exist. by using the deer mouse infectious model, the in vivo inhibitory potential of ribavirin, human anti-snv immune plasma (hip), an anti-beta3 antibody (reopro) and a polyclonal rabbit anti-recombinant nucleocapsid (n) antibody against snv was investigated. concurrent intraperitoneal a ... | 2007 | 17251568 |
| andes hantavirus as possible cause of disease in travellers to south america. | hantaviruses in europe and asia cause haemorrhagic fever with renal syndrome and epidemic nephritis (mortality rate <1-15%). new strains of hantaviruses cause hantavirus pulmonary syndrome (hps) from canada to south america. andes virus mortality rate is about 30% in chile. | 2007 | 17161316 |
| serological evidence for borna disease virus infection in humans, wild rodents and other vertebrates in finland. | borna disease virus (bdv) can infect many vertebrate species, including humans. bdv infection may lead to meningoencephalomyelitis in animals. an association with human neuropsychiatric diseases has been reported, but the causal relationship between bdv and human disease remains unclear. | 2007 | 17129759 |
| rapid immunochromatographic test for hantavirus andes contrasted with capture-igm elisa for detection of andes-specific igm antibodies. | hantavirus is associated with hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). the clinical diagnosis of hantavirus infections has been confirmed routinely by the immunofluorescence antibody assay (ifa) or enzyme-linked immunosorbent assay (elisa). a rapid and easy diagnostic test for hantavirus infection is required. a new immunochromatographic assay for hantavirus, poc-puu, useful for the diagnosis of epidemic nephrophaty associated with hantavirus puumala ... | 2007 | 17133547 |
| cross-reactive immune responses in mice after genetic vaccination with cdna encoding hantavirus nucleocapsid proteins. | hantaviruses cause hemorrhagic fever with renal syndrome (hfrs) in about 150,000 individuals in eurasia, and several hundred cases of hantavirus pulmonary syndrome (hps) on the american continent annually. there is consequently a need for rapid diagnostics and effective prevention of hantaviral infections. in this study we have performed dna-vaccination of mice with full-length genes encoding the immunogenic nucleocapsid protein (np) of puumala (puuv), seoul (seov) and sin nombre virus (snv). th ... | 2007 | 17110000 |
| increased permeability of human endothelial cell line ea.hy926 induced by hantavirus-specific cytotoxic t lymphocytes. | hantavirus infection causes two human diseases, hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. the typical feature of these diseases is increased permeability in microvascular beds in the kidneys and the lungs, respectively. the mechanism of capillary leakage, however, is not understood. some evidence suggests that hantavirus disease pathogenesis is immunologically mediated by cytotoxic t lymphocytes and other immune cells in target organs producing inflammatory cytokin ... | 2007 | 16979772 |
| puumala hantavirus in slovenia: analyses of s and m segment sequences recovered from patients and rodents. | in slovenia, the co-existence of dobrava and puumala (puuv) hantaviruses in a single endemic region has been demonstrated. this study presents selected slovenian hfrs cases caused by puuv combined with genetic analysis of viral genome sequences recovered from clinical specimens and tissue samples of clethrionomys glareolus (bank voles). serum samples from nine hfrs patients were included in the study. rodents study sites were selected with regard to the hfrs cases. partial s segment sequences we ... | 2007 | 16997412 |
| disease emergence in multi-host epidemic models. | most pathogens are capable of infecting multiple hosts. these multiple hosts provide many avenues for the disease to emerge. in this investigation, we formulate and analyse multi-host epidemic models and determine conditions under which the disease can emerge. in particular, sis and sir epidemic models are formulated for a pathogen that can infect n different hosts. the basic reproduction number is computed and shown to increase with n, the number of hosts that can be infected. therefore, the po ... | 2007 | 17012365 |
| hsp70 gene fused with hantavirus s segment dna significantly enhances the dna vaccine potency against hantaviral nucleocapsid protein in vivo. | heat shock proteins (hsps) have been shown to act as adjuvants when coadministered with peptide antigens or given as fusion proteins and enhance the vaccination efficiency. to evaluate the enhancement of the potency of hantaan virus (htnv) nucleocapsid protein (np) immunogenicity by heat shock protein 70 (hsp70), we developed a novel chimeric htnv s-hsp70 dna vaccine plasmid by genetically linking hsp70 gene to the full-length htnv s segment dna (htnv s dna). c57bl/6 mice were immunized with thi ... | 2007 | 16935395 |
| [virological surveillance on hemorrhagic fever with renal syndrome in hunan province in 2006]. | to understand the rate of viral carrying status among rodents as well as genotypes and distribution of hantaviruses (hv) isolated in hunan province. | 2007 | 18476580 |
| [etiology of infectious diseases in pregnant women with symptoms of fever and lung syndrome]. | 31 samples of blood serum of pregnant women with acute respiratory virus infection and pneumonia symptoms who had been administered into hospital were analyzed. antibacterial and antifungal treatment was ineffective. autumn-winter-spring seasonality was detected. in connection with above-mentioned, an attempt was made to find out the etiology of a disease using viroloy methods. primers of hantaviruses, crimea-and-congo hemorrhage fever, coronaviruses, sars, leptospirosis and virus hepatitis. gen ... | 2007 | 18712099 |
| [the role of natural environment in spreading of hantavirus--model of the correlation between host, pathogen and human infections]. | the environmental changes caused by humans influence ecosystem and thus have significant impact on occurrence of emerging and re-emerging diseases. the hantavirus infection belong to the one of them. the aim of this paper was to present current knowledge about relationship between hantavirus, their natural host and the spread of the infection to people. rodents constitute both the natural host of the hantaviruses and the reservoir of hantavirus for environment. circulation of the virus in the ro ... | 2007 | 18572496 |
| stratifying land use/land cover for spatial analysis of disease ecology and risk: an example using object-based classification techniques. | landscape epidemiology has made significant strides recently, driven in part by increasing availability of land cover data derived from remotely-sensed imagery. using an example from a study of land cover effects on hantavirus dynamics at an atlantic forest site in eastern paraguay, we demonstrate how automated classification methods can be used to stratify remotely-sensed land cover for studies of infectious disease dynamics. for this application, it was necessary to develop a scheme that could ... | 2007 | 18686252 |
| can modeling of fine-scale spatial patterns of environmental markers of zoonotic infections enhance disease prevention and clinical outcomes? | 2007 | 18165510 | |
| a spatial model of shared risk for plague and hantavirus pulmonary syndrome in the southwestern united states. | plague and hantavirus pulmonary syndrome (hps) are severe, often fatal diseases in humans that share a broad epidemiologic focus in the southwestern united states. prevention of these diseases relies heavily on education and reducing rodent abundance in peridomestic environments. resources for these activities are limited. therefore, identifying areas sharing elevated risk for these two relatively rare but severe diseases could be useful for targeting limited public health resources. using logis ... | 2007 | 18165511 |
| mode of infection of hokkaido virus (genus hantavirus) among grey red-backed voles, myodes rufocanus, in hokkaido, japan. | hokkaido virus (hokv) is a member of the genus hantavirus, in the family bunyaviridae. to investigate hokv infection in the host myodes rufocanus, the grey red-backed vole, 199 animals were captured at tobetsu (october 2004 and july 2005) and nakagawa (october 2004) in hokkaido, japan, for detection of antibody, antigen, and viral rna. in the surveys in tobetsu (2004) and nakagawa (2004), seropositive animals were detected at a frequency of 6.0% (5/84) and 10.4% (5/48), respectively. no seroposi ... | 2007 | 18037785 |