Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| affinity of synthetic peptides for the hsv-2 ribonucleotide reductase r1 subunit measured with an iodinated photoaffinity peptide. | the herpes simplex virus (hsv) ribonucleotide reductase comprises two nonidentical subunits, r1 and r2, which associate to form the active holoenzyme. a sensitive binding assay was developed to measure the affinity of inhibitory peptides for the hsv r1 subunit. the assay involved the use of a photoreactive radioligand [4'-azido-phe328,3',5'-125i-tyr329] hsv r2-(328-337), an analogue of the decapeptide ser-tyr-ala-gly-ala-val-val-asn-asp-leu which corresponds to the c-terminal sequence (328-337) ... | 1994 | 7978273 |
| recurrence rates in genital herpes after symptomatic first-episode infection. | to evaluate the frequency of reactivation of genital herpes infection and to identify predictors for recurrence. | 1994 | 7978697 |
| kinetic analysis of the interaction between the diphosphate of (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine, ddctp, azttp, and fiautp with human dna polymerases beta and gamma. | the inhibitory effects of the diphosphate of (s)-1-(3-hydroxy-2-phosphonylmethoxy-propyl)cytosine (hpmpcpp) toward human dna polymerases beta and gamma were studied. the ki values of hpmpcpp were compared with the ki values of the triphosphates of 3'-azidothymidine (azttp), 2',3'-dideoxycytidine (ddctp) and 5-iodo-2'-fluoroarabinosyluridine (fiautp). the ki values toward dna polymerase beta in increasing order were 1.32, 1.43, 140, and 520 microm for ddctp, fiautp, azttp and hpmpcpp, respectivel ... | 1994 | 7986213 |
| a potent peptidomimetic inhibitor of hsv ribonucleotide reductase with antiviral activity in vivo. | herpes simplex viruses (hsv) types 1 and 2 encode their own ribonucleotide reductases (rnrs) (ec 1.17.4.1) to convert ribonucleoside diphosphates into the corresponding deoxyribonucleotides. like other iron-dependent rnrs, the viral enzyme is formed by the reversible association of two distinct homodimeric subunits. the carboxy terminus of the rnr small subunit (r2) is critical for subunit association and synthetic peptides containing these amino-acid sequences selectively inhibit the viral enzy ... | 1994 | 7990963 |
| [studies on antiviral agents: synthesis of tai-ding-an analogs]. | tai-ding-an (3-phthalimido-2-oxo-n-butyraldehyde bisthiosemicarbazone, tda) is a synthetic antiviral drug developed by our institute. because of its low water/lipid solubility. tai-ding-an is limited to topical treatment of herpes viruses infected skin diseases and of condylomata acuminata (ginital warts), one of sexual transmitted diseases (std), caused by human papilloma virus (hpv). ten tda derivatives iiib-e, va, vb, viia-e have been synthesized in order to search for compounds with higher a ... | 1994 | 7992623 |
| male circumcision and common sexually transmissible diseases in a developed nation setting. | to determine whether the circumcision status of men affected their likelihood of acquiring sexually transmissible diseases (stds). | 1994 | 8001942 |
| evidence for a multistep mechanism for cell-cell fusion by herpes simplex virus with mutations in the syn 3 locus using heparin derivatives during fusion from within. | addition of heparin-na+ as well as related substances of high and intermediate mw (arteparon and polyanion sp54) 3 h after infection inhibit fusion from within (ffwi) induced by hsv strains with mutations in the syn 3 locus only. the concentration of heparin-na+ required to inhibit ffwi is 10-fold higher (1 mg/ml) than that needed to inhibit adsorption. instead of fusion, cell rounding is observed. the effect is readily reversible. a low mw heparin disaccharide is ineffective. neomycin, at a con ... | 1994 | 8002784 |
| protein-specific cervical antibody responses to primary genital herpes simplex virus type 2 infections. | antibodies to herpes simplex virus (hsv) have been demonstrated in cervicovaginal secretions but have not been analyzed for their viral protein targets, prevalence, isotype, or kinetics of development. a method was devised to collect cervical secretions from women with primary genital hsv-2. by western blot, cervical igg and iga responses to hsv-2 proteins vp5, gb, and gd were detected in most patients within 2 weeks of onset and to gc/ge within 3 weeks. cervical igm and igg responses to gg, vp1 ... | 1994 | 8014499 |
| analysis of the thymidine kinase of a herpes simplex virus type 1 isolate that exhibits resistance to (e)-5-(2-bromovinyl)-2'-deoxyuridine. | the mechanism responsible for the decreased sensitivity of a clinical herpes simplex virus type 1 (hsv-1) isolate, hsv-145, to (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) was examined. measurements of 50% inhibitory doses of several drugs demonstrated that although hsv-145 was sensitive to phosphonoacetic acid, adenine arabinoside and acyclovir, its sensitivity to bvdu and 5-(2-chloroethyl)-2'-deoxyuridine was significantly less than that normally observed for hsv-1. analysis of the thymidylate ... | 1994 | 8021603 |
| herpes simplex virus type 2 ascending myeloradiculitis: mri findings and rapid diagnosis by the polymerase chain method. | 1994 | 8021686 | |
| comparative cytostatic activity of different antiherpetic drugs against herpes simplex virus thymidine kinase gene-transfected tumor cells. | a series of selective antiherpetic compounds were found to exert pronounced cytostatic activity against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) thymidine kinase (tk) gene-transfected mammary carcinoma fm3a cells. based on their potency and mechanism of cytostatic action, the antiherpetic compounds could be divided into two different classes. the first class encompasses (e)-5-(2-bromovinyl)-2'-deoxyuridine and structurally related analogues thereof [i.e., the cytosine derivative (e ... | 1994 | 8022417 |
| stochastic curtailing and conditional power in matched case-control studies. | new methodology for early stopping of observational epidemiologic studies is developed. to indicate the likelihood of a positive result at the end of a study, the conditional power (cp) is calculated using an approach similar to that of halperin et al. given the current result, the conditional probability of a positive result for the entire study is determined. an expression relating the new approach to an approach given by lan and wittes is derived. as an example, the cp is calculated based on ... | 1994 | 8023041 |
| effects of the angiotensin ii receptor antagonist losartan on herpes simplex virus-type 2 infection of cultured vero and cardiac neonatal myocytes. | previous studies indicate that captopril, an angiotensin ii converting enzyme inhibitor, attenuates cardiomyopathy in a murine viral myocarditis model. accordingly, we investigated the ability of captopril as well as angiotensin ii (aii) and losartan, a nonpeptide aii receptor antagonist, to alter infection or replication of herpes simplex virus- type 2 (hsv-2) in cultured cardiac and vero cells. neither captopril nor aii influenced the ability of hsv-2 to replicate in either cell type. losartan ... | 1994 | 8028446 |
| antiviral activity of a sulphated polysaccharide from the red seaweed nothogenia fastigiata. | the antiviral activity of polysaccharide fractions obtained from water extracts of the red seaweed nothogenia fastigiata was investigated. fraction f6, corresponding to a sulphated xylomannan, was found to inhibit efficiently the replication of herpes simplex virus type 1 (hsv-1). furthermore, f6 selectively inhibited the replication of several other enveloped viruses including herpes simplex virus type 2, human cytomegalovirus (hcmv), respiratory syncytial virus, influenza a and b virus, junin ... | 1994 | 8031312 |
| prevalence of antibodies to herpes simplex virus in pregnant women in stockholm in 1969, 1983 and 1989: implications for std epidemiology. | prevalence of antibody to herpes simplex virus types 1 and 2 was assessed in consecutive serum samples from a total of 3700 women pregnant in 1969, 1983, or 1989 from the same catchment area in stockholm. there was little change in seroprevalence of antibody to herpes simplex type 1 in the 3 groups, but age-adjusted herpes simplex virus type 2 antibody prevalence was 19, 33, and 33% respectively. increase in type 2 seropositivity with age was slight and similar in 1969 and 1989, but steep in 198 ... | 1994 | 8031912 |
| selected epidemiological features of herpes genitalis in israel based on laboratory data. | the incidence in israel of genital infection with herpes simplex virus (hsv) has been increasing steadily over the last two decades. during the years 1973-91, 1,508 patients had an hsv infection confirmed by viral culturing. the yearly incidence of new cases rose from sporadic cases in the seventies to 32.4 cases per 1,000 patients in 1990. the age distribution pattern did not change since 1980 and showed a peak incidence in subjects aged 20-40 years. the increase of hsv type 2 (hsv-2) infection ... | 1994 | 8034486 |
| a seroprevalence study of herpes virus type 2 infection in israeli women: implications for routine screening. | the aim of the study was to obtain data on the prevalence of herpes simplex virus type 2 (hsv-2) in selected populations of women and to identify groups that might benefit from routine prenatal screening. the prospective seroprevalence study was performed in 1,921 women in israel during the period 1986-90. four different population groups of women 17-60 years old were included. groups 1 and 2 comprised 1,214 healthy female government employees and kibbutz and moshav residents, and groups 3 and 4 ... | 1994 | 8034487 |
| the inter-relationship of herpes virus, papilloma 16/18 virus infection and pap smear pathology in israeli women. | a previous survey of the seroprevalence of the herpes virus type 2 (hsv-2) infection in israel provided us with an opportunity to study a) the prevalence of human papillomavirus (hpv) 16/18 in selected groups of women; b) the correlation between the infection with hsv-2, hpv-16/18 and papanicolaou (pap) pathology; and c) to identify groups of women who might benefit from routine pap screening. four different population groups of women aged 17-60 years were studied: groups 1 and 2 comprised healt ... | 1994 | 8034488 |
| vaccine therapy for ocular herpes simplex virus (hsv) infection: periocular vaccination reduces spontaneous ocular hsv type 1 shedding in latently infected rabbits. | periocular vaccination of rabbits with preexisting herpes simplex virus type 1 (hsv-1) latent infection with recombinant hsv-2 glycoproteins b and d (gb2 and gd2) plus adjuvant significantly reduced ocular viral shedding. rabbits were infected in both eyes with hsv-1 strain mckrae. following hsv-1 infection and the establishment of latency (28 days postinfection), rabbits were given a periocular subconjunctival vaccination three times at 3-week intervals. beginning 3 weeks after the final vaccin ... | 1994 | 8035508 |
| cervical antibodies in patients with oral herpes simplex virus type 1 (hsv-1) infection: local anamnestic responses after genital hsv-2 infection. | herpes simplex virus (hsv)-specific immunoglobulin a, immunoglobulin g, and secretory-component-containing immunoglobulins were identified in cervical and salivary secretions from six subjects with oral hsv type 1 (hsv-1) infections. anamnestic cervical and salivary antibody responses were detected in two hsv-1-seropositive women with newly acquired genital hsv-2 infections. these data implicate the common mucosal immune system in antibody responses to hsv. | 1994 | 8035526 |
| interferon-gamma in cerebrospinal fluid from patients with viral and bacterial meningitis. | interferon (ifn)-gamma was analysed immunologically in cerebrospinal fluid (csf) sampled in the acute phase from 27 patients (15-66 years) with viral meningitis and from 18 patients (0.5-90 years) with bacterial meningitis. increased csf concentrations were observed in 19/27 viral and in 13/18 bacterial cases. csf-ifn-gamma did not distinguish between viral and bacterial meningitis. five of 8 patients with meningitis due to herpes simplex virus type 2 (hsv-2) had csf-ifn-gamma levels above the h ... | 1994 | 8036469 |
| successful treatment of progressive mucocutaneous infection due to acyclovir- and foscarnet-resistant herpes simplex virus with (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (hpmpc). | the acyclic nucleoside phosphonate (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (hpmpc) was used topically for the treatment of persistent mucocutaneous infections in two cases. one patient with aids suffered from a perineal lesion due to infection with herpes simplex virus type 2 (hsv-2) and did not respond to acyclovir and was intolerant of foscarnet. a bone marrow transplant recipient developed orofacial lesions due to infection with herpes simplex virus type 1 (hsv-1) that failed to r ... | 1994 | 8038312 |
| apparent increase in the prevalence of herpes simplex virus type 1 genital infections among women. | 1994 | 8039792 | |
| the current trend in genital herpes. progress in prevention. | recent serosurveys indicate that the prevalence of genital herpes has continued to increase even during the decade of hiv. much of this continued transmission is due to the difficulty of identifying the subclinical carrier of hsv-2. the development of serologic assays that accurately distinguish hsv-1 from hsv-2 infection now allow such persons to be identified, and recent studies indicate almost all hsv-2 seropositives have symptoms and signs of reactivation hsv-2. moreover, over 50% will shed ... | 1994 | 8042114 |
| cyclocreatine (1-carboxymethyl-2-iminoimidazolidine) inhibits the replication of human herpes viruses. | the creatine kinase/creatine phosphate (ck/crp) system plays an important role in cellular energy homeostasis. ck isoenzymes, which reversibly generate atp from crp, are compartmentalized at cellular sites where energy is produced or utilized. it has been noted that the expression of ck is induced in cells infected by several dna viruses, implicating a role for cellular energy modulation as an important step for efficient viral replication. a ck substrate analog, 1-carboxymethyl-2-iminoimidazoli ... | 1994 | 8042860 |
| identification of a target protein of us3 protein kinase of herpes simplex virus type 2. | herpes simplex virus type 2 (hsv-2) gene us3 has been shown to encode a serine-threonine protein kinase. in this study, we have tried to identify target proteins of the us3 protein kinase using a us3 lacz insertion mutant of hsv-2. when permeabilized cells were labelled with [gamma-32p]atp under the optimum conditions for the us3 enzyme, the most striking difference between wild-type hsv-2 strain 186- and mutant-infected cells was observed in the phosphorylation of proteins ranging in m(r) value ... | 1994 | 8046410 |
| the interaction of glycoprotein c of herpes simplex virus types 1 and 2 with the alternative complement pathway. | glycoprotein c (gc) of herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) binds the human complement fragment c3b, but the two proteins differ in their ability to bind c3b on infected cell surfaces. in addition, gc-1, but not gc-2, accelerates the decay of the alternative pathway c3 convertase, thereby affecting later steps of the complement cascade. previously, we constructed linker insertion and deletion mutants of gc-1 and gc-2 and used transient transfection to express mutant proteins in ... | 1994 | 8053154 |
| acute retinal necrosis caused by reactivation of herpes simplex virus type 2. | acute retinal necrosis is a severe form of necrotizing retinitis. acute retinal necrosis has been demonstrated to be caused by varicella-zoster virus and herpes simplex virus type 1. we treated three patients with acute retinal necrosis apparently caused by recrudescence of latent herpes simplex virus type 2. primary viral infection was probably congenital, with documented perinatal herpes simplex virus type 2 infection in two patients. bilateral chorioretinal scars were present in two patients, ... | 1994 | 8053466 |
| synthesis and antiviral activity of three pyrazole analogues of distamycin a. | the synthesis of three new monopyrazole analogues of the antiviral compound distamycin a is reported. suitably protected 4-amino-1-methylpyrrole-2-carboxylic acid and 3-amino-1-methylpyrazole-5-carboxylic acid derivatives were chosen as starting materials. the construction of the trimeric polyamide framework was accomplished by assembly of the monomeric precursors under condensing conditions by analogy with our previous methodology, although with significant improvements in some pivotal steps. a ... | 1994 | 8060726 |
| epidemiology of genital herpes. | new serological methods, based on the detection of type-specific antibodies to herpes simplex virus (hsv) glycoproteins, have allowed a reappraisal of the prevalence of genital herpes. during the 1980s, genital herpes continued to be an infection of epidemic proportions in many developed countries. in the usa, although there was evidence of a decline in the age-specific prevalence of hsv type 1 (hsv-1) infection, the seroprevalence of hsv type 2 (hsv-2) has continued to increase. among those att ... | 1994 | 8063020 |
| undocumented history of maternal genital herpes followed by neonatal herpes meningitis. | 1994 | 8064427 | |
| synthesis of some acylphosphonate thiosemicarbazones and evaluation of their antiviral activity. | reaction of dimethyl acylphosphonates (1) with thiosemicarbazide afforded the corresponding dimethyl acylphosphonate thiosemicarbazones (4). lithium methyl acylphosphonate thiosemicarbazones (5) could be prepared either by monodealkylating compounds 4 by lithium bromide or by reacting lithium methyl acylphosphonates with thiosemicarbazide. dihydrogen acylphosphonate thiosemicarbazones (6) could be obtained by reacting acylphosphonic acids (3) with thiosemicarbazide. the alternative approach to 6 ... | 1994 | 8068818 |
| epithelial interleukin-11. regulation by cytokines, respiratory syncytial virus, and retinoic acid. | interleukin-11 (il-11) is a pleiotropic cytokine with effects that overlap with il-6. to determine if il-11 is produced by epithelial cells, we determined whether human alveolar a549 cells and airway 9hte cells produce il-11. we also determined whether retinoic acid (ra) altered this il-11 production. unstimulated cells produced low levels of il-11, while il-1, transforming growth factor (tgf-beta 1), and respiratory syncytial virus (rsv) stimulated il-11 protein production and mrna accumulation ... | 1994 | 8071352 |
| synthesis and antiviral activity evaluation of some aminoadamantane derivatives. | the synthesis of some spiro[cyclopropane-1,2'-adamantan]-2-amines and methanamines and some spiro[pyrrolidine-2,2'-adamantanes] is described. the title compounds were evaluated against a wide range of viruses (influenza a, influenza b, parainfluenza 3, rsv, hsv-1, tk- hsv-1, hsv-2, vaccinia, vesicular stomatitis, polio 1, coxsackie b4, sindbis, semliki forest, reo 1, hiv-1, and hiv-2), and some of them (compounds 6b, 6c, 9a, 16a, 16b, and 17) inhibited the cytopathicity of influenza a virus at a ... | 1994 | 8071937 |
| [acute benign lymphocytic meningitis caused by type 2 herpes simplex virus]. | 1994 | 8072966 | |
| detection of human alpha-herpesvirus dna using consensus primers and specific probes. | a simple and rapid diagnostic assay system was developed for detecting and identifying human alpha-herpesviruses, such as herpes simplex virus types 1 and 2 and varicella-zoster virus, by polymerase chain reaction. this system was based on an amplification step, using primers that bind the dna polymerase consensus sequence of alpha-human herpesviruses, and a detection step, using non-radioactive virus-specific probes. this method could be used to amplify any human alpha-herpesviruses, and each v ... | 1994 | 8073876 |
| cell type and cell state determine differential in vitro growth of non-neurovirulent icp34.5-negative herpes simplex virus types 1 and 2. | the herpes simplex virus (hsv) gene rl1 encodes the protein icp34.5, which is a specific neurovirulence factor. null mutants in rl1 fail to replicate in the central nervous system of mice and are therefore totally non-neurovirulent. additionally, they fail to replicate in neurons of the peripheral nervous system, although they are capable of establishing and reactivating from a latent infection. as the precise function of icp34.5 in hsv-neuronal interactions is unknown, we have studied the role ... | 1994 | 8077935 |
| differentiation of herpes simplex virus 1 and 2 in cerebrospinal fluid of patients with hsv encephalitis and meningitis by stringent hybridization of pcr-amplified dnas. | differentiation of herpes simplex virus (hsv) types 1 and 2 in cerebrospinal fluid of 17 patients with serologically diagnosed hsv encephalitis and meningitis or acute limbic encephalitis was determined by stringent hybridization of polymerase chain reaction--amplified dnas. ten of 17 patients were positive; six with hsv 1 isolates and four with hsv 2 isolates. we detected hsv type 1 in two cases of meningitis, although meningitis is generally thought to be caused by type 2. additionally, hsv ty ... | 1994 | 7799000 |
| detection of the bc 24 transforming fragment of the herpes simplex virus type 2 (hsv-2) dna in cervical carcinoma tissue by polymerase chain reaction (pcr). | the polymerase chain reaction (pcr) was used to investigate samples from indonesian and swedish patients with cervical intraepithelial neoplasia grade iii (cin iii), squamous cell carcinoma or adenocarcinoma of the cervix for the presence of a transforming fragment (bc 24) of herpes simplex virus type 2 (hsv-2) dna. the pcr test for hsv-2 dna was more sensitive than the infectivity endpoint titer in a cell culture system and no cross reactivity was found with either varicella-zoster virus, cytom ... | 1994 | 7794306 |
| correlation of cervical cancer mortality with reproductive and dietary factors, and serum markers in china. | cervical cancer is the second leading cause of cancer death among chinese women. within china, a considerable geographical variation in mortality rates has been observed, but the reasons are not well understood. | 1994 | 7721512 |
| treatment of acyclovir-unresponsive cutaneous herpes simplex virus infection with topically applied sp-303. | the naturally occurring polyphenolic biopolymer sp-303 has in vitro activity against both hsv-1 and hsv-2, including strains that are resistant to acyclovir. nine aids patients with acyclovir-unresponsive mucocutaneous herpes simplex virus infection were treated with thrice daily topical sp-303t ointment in an open-label pilot study. although a transient decrease in lesion size was observed in 4 patients during study drug therapy, and 3 patients sustained a quantitative decrease in virus burden, ... | 1994 | 7710268 |
| approaches to maximizing stable expression of alpha 1-antitrypsin in transformed cho cells. | a variety of approaches to maximizing the production of recombinant human alpha 1-antitrypsin (aat) in chinese hamster ovary (cho) cells have been investigated. the highly active and inducible human cytomegalovirus immediate early (ie) promoter/enhancer was used to drive transcription of a recombinant aat gene in transiently transfected and stably transformed cho cells. the aat gene was modified to incorporate highly efficient 3'rna processing signals from the herpes simplex virus type 2 ie gene ... | 1994 | 7764427 |
| posttherapy suppression of genital herpes simplex virus (hsv) recurrences and enhancement of hsv-specific t-cell memory by imiquimod in guinea pigs. | imiquimod, an immunomodulator with no direct in vitro antiviral activity, has in vivo anti-herpesvirus activity by inducing interferon and enhancing other only partially defined immune responses. imiquimod treatment of primary genital herpes simplex virus (hsv) infection in guinea pigs reduces the level of genital disease by 90%. we further investigated its utility as suppressive therapy of recurrent disease in animals that had recently recovered from primary genital hsv-2 disease. imiquimod adm ... | 1994 | 7811019 |
| simultaneous measurement of antibodies to epstein-barr virus, human herpesvirus 6, herpes simplex virus types 1 and 2, and 14 enteroviruses in chronic fatigue syndrome: is there evidence of activation of a nonspecific polyclonal immune response? | as a test of the hypothesis that elevated titers of viral antibodies in patients with chronic fatigue syndrome (cfs) are due to a nonspecific polyclonal immune response, antibodies to epstein-barr virus (ebv), human herpesvirus 6 (hhv-6), and 14 enteroviruses in 20 patients with cfs and 20 age- and gender-matched controls were simultaneously measured. similarly, titers of igg to herpes simplex virus (hsv) types 1 and 2 were measured in 18 of these cases and in the respective controls. igg to ebv ... | 1994 | 7811864 |
| seroprevalence and incidence of sexually transmitted diseases in a rural ugandan population. | the aim of the study was to determine in a rural population the age- and sex-specific prevalence and incidence rates of serological reactivity of 5 common sexually transmitted diseases (stds) and their association with hiv-1 antibody status. of the adult population of two villages (529 adults aged 15 years or more) 294 provided an adequate blood specimen both on enrollment and at 12 months. the sera were tested at 3 collaborating laboratories for antibodies against hiv-1, treponema pallidum, hae ... | 1994 | 7819350 |
| comparison of various transport media for viability maintenance of herpes simplex virus, respiratory syncytial virus, and adenovirus. | seven viral transport media (vtm) were compared for effectiveness in preserving the infectivity of herpes simplex virus (hsv), respiratory syncytial virus (rsv), and adenovirus. the media tested were richards viral transport, sucrose-phosphate-glutamate (spg), virocult, hh medium, tryptose phosphate broth, cell culture medium, and bartel's viral transport. two laboratory strains of hsv (mcintyre and 333) and two clinical isolates (a0301 and a0386), comprising two hsv-1 types and two hsv-2 types, ... | 1994 | 7820992 |
| a sensitive assay system screening antiviral compounds against herpes simplex virus type 1 and type 2. | a highly sensitive and accurate assay system was developed for in vitro evaluation of anti-herpes simplex virus (anti-hsv) agents using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) and human embryonic lung fibroblast (mrc-5) cells. this assay system was found to be highly sensitive for both hsv-1 and -2. confluent mrc-5 cells were infected with either hsv-1 kos strain or hsv-2 g strain of 25 tcid50 in the presence of various concentrations of test compounds. the optical den ... | 1994 | 7822458 |
| [4'-branched nucleosides. ii. synthesis of 4'-hydroxymethyl derivatives of 2',3'-anhydronucleosides of the ribo- and lyxo-series]. | a general synthetic method for 4'-hydroxymethyl-2',3'-anhydronucleosides from 1,2-o-isopropylidene-4-hydroxymethyl-alpha-d-xylofuranose is described. the condensation of 1,2-di-o-acetyl-3-o-methanesulphonyl-4-benzoyloxymethyl-5-o- benzoyl-d-xylofuranose with trimethylsilyl derivatives of n6-benzoyladenine and n2-palmitoylguanine in the presence of stannic chloride resulted in the corresponding nucleosides. after their treatment with nh4oh-etoh, corresponding 2',3'-riboanhydronucleosides were iso ... | 1994 | 7826408 |
| teenage obstetric and gynaecological problems in an african city. | to measure the prevalence of sexually transmitted diseases (std), pelvic inflammatory disease (pid), cervical cancer, pregnancy and use of contraception in teenagers, and to determine socioeconomic factors associated with these conditions to aid planners of medical services and promotion of sexual health. | 1994 | 7834712 |
| p53 expression and genetic evidence for viral infection in intraepithelial neoplasia of the uterine cervix. | infections with high-risk strains of human papillomaviruses (hpvs) and with herpes simplex virus type 2 (hsv 2), as well as inactivation of the p53 tumor suppressor gene, are important cofactors in cervical carcinogenesis. we analyzed 41 paraffin-embedded cervical intraepithelial lesions, including 25 cases of low-grade cervical intraepithelia neoplasia (cin), and 16 cases of high-grade cin for the presence of hpv 16/18 and hsv 2 genomic sequences and for the nuclear accumulation of the p53 prot ... | 1994 | 7835772 |
| 1-[((s)-2-hydroxy-2-oxo-1,4,2-dioxaphosphorinan-5-yl)methyl] cytosine, an intracellular prodrug for (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine with improved therapeutic index in vivo. | 1-[((s)-2-hydroxy-2-oxo-1,4,2-dioxaphosphorinan-5-yl)methyl]cytosi ne (cyclic [chpmpc]) was evaluated as a novel antiviral agent in comparison with (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (hpmpc). evaluation for in vitro activity against herpes simplex virus type 2 in ma-104 and mrc-5 cells showed that both chpmpc and hpmpc have comparable activities and cytotoxicities. chpmpc was found to be stable on incubation in human plasma and human liver homogenates. intracellular metabolism s ... | 1994 | 7840575 |
| herpes simplex virus type 2 meningitis and associated genital lesions in a three-year-old child. | 1994 | 7845725 | |
| the herpes simplex virus type 2 gene which encodes the large subunit of ribonucleotide reductase has unusual regulatory properties. | expression of herpes simplex virus type 2 (hsv-2) encoded ribonucleotide reductase (rr) is required for growth of the virus in non-dividing cells. the functional enzyme is composed of a large (rra) and small (rrb) subunit and the enzyme is expressed as a delayed early activity. the promoter of rra contains a cis-acting motif (taatgarat) which resembles those found in immediate early (ie) genes suggesting rra is an ie gene. when primate cells were infected with hsv-2, low levels of rra transcript ... | 1994 | 7856314 |
| antibody to herpes simplex virus type 2 as serological marker of sexual lifestyle in populations. | to examine the epidemiology of antibody to herpes simplex virus type 2 and to assess its suitability as a serological marker of sexual behaviour in populations with high and low prevalences. | 1994 | 7866079 |
| [distribution of herpes simplex virus type 1 and 2 genomes in the human spinal ganglia]. | herpes simplex virus (hsv) is well known for its propensity to cause recurrent oral or genital mucosal infections in humans. hsv-1 is involved primarily in oral lesions, whereas hsv-2 is more frequently involved in genital lesions. based on this, it is thought that hsv-1 may produce latent infections in trigeminal ganglia, and hsv-2 in the sacral ganglia. however the distribution pattern of latent hsv-1 and hsv-2 infections in spinal ganglia remains unknown. using the polymerase chain reaction w ... | 1994 | 7868053 |
| [the determination of the antigenic activity of recombinant virus-specific polypeptides from the herpes simplex virus types 1 and 2 and their use in immunoenzyme analysis]. | the structural and nonstructural recombinant proteins of hsv type 1 and type 2 were expressed in e. coli by fusion with cro-beta-galactosidase proteins. these proteins containing amino acid sequences of icp4, icp27, icp47, icp22, major capsid protein and major dna-binding proteins of hsv types 1 and 2 reacted in immunoblotting with the corresponding anti-hsv sera from hyperimmune rabbits. the nonstructural recombinant proteins can be used for enzyme immunoassay detection of hsv1 or hsv2 type-spe ... | 1994 | 7517084 |
| effect of mercuric chloride on macrophage-mediated resistance mechanisms against infection with herpes simplex virus type 2. | macrophages play an important role in the early, nonspecific resistance to infection with herpes simplex virus. mercuric chloride (hgcl2) accumulates in macrophages and has in certain concentrations a marked influence on the functional capacity of these cells. therefore the influence of hgcl2 on resistance to generalized infection with herpes simplex virus type 2 (hsv-2) in mice and its effect on the hsv-2-induced activation of macrophages in vitro was examined. mice injected intraperitoneally w ... | 1994 | 7526487 |
| the n-7-substituted acyclic nucleoside analog 2-amino-7-[(1,3-dihydroxy-2-propoxy)methyl]purine is a potent and selective inhibitor of herpesvirus replication. | 2-amino-7-[(1,3-dihydroxy-2-propoxy)methyl]purine (compound s2242) represents the first antivirally active nucleoside analog with the side chain attached to the n-7 position of the purine ring. compound s2242 strongly inhibits the in vitro replication of both herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) (50% effective concentration [ec50], 0.1 to 0.2 microgram/ml), varicella-zoster virus (ec50, 0.01 to 0.02 microgram/ml) and thymidine kinase (tk)-deficient strains of hsv (ec50, 0.4 mic ... | 1994 | 7695251 |
| identification of functional regions of herpes simplex virus glycoprotein gd by using linker-insertion mutagenesis. | glycoprotein gd is a component of the herpes simplex virus (hsv) envelope essential for virus entry into susceptible cells. previous studies using deletion and point mutations identified a functional domain of hsv-1 gd (gd-1) from residues 231 to 244. however, many of the deletion mutations had global effects on gd-1 structure, thus precluding assessment of the functional role of large portions of the protein. in this study, we constructed a large panel of linker-insertion mutants in the genes f ... | 1994 | 7511173 |
| antigenic specificities of human cd4+ t-cell clones recovered from recurrent genital herpes simplex virus type 2 lesions. | lesions resulting from recurrent genital herpes simplex virus (hsv) infection are characterized by infiltration of cd4+ lymphocytes. we have investigated the antigenic specificity of 47 hsv-specific cd4+ t-cell clones recovered from the hsv-2 buttock and thigh lesions of five patients. clones with proliferative responses to recombinant truncated glycoprotein b (gb) or gd of hsv-2 or purified natural gc of hsv-2 comprised a minority of the total number of hsv-specific clones isolated from lesions ... | 1994 | 7512152 |
| long-term foscarnet therapy not associated with the development of foscarnet-resistant human immunodeficiency virus type 1 in an acquired immunodeficiency syndrome patient. | sequential human-immunodeficiency virus type 1 (hiv-1) isolates from an acquired immunodeficiency syndrome (aids) patient on long-term foscarnet and zidovudine therapy were examined for the emergence of foscarnet resistance. one isolate obtained before therapy, and three post-foscarnet therapy hiv isolates had similar foscarnet sensitivity profiles, despite the emergence of foscarnet-resistant herpes simplex virus type 2 (hsv-2) during the period of therapy. virion reverse transcriptases from th ... | 1994 | 7512615 |
| detection of herpes simplex virus type 2 bgl ii n fragment in paraffin-embedded cervical tissue sections using nested polymerase chain reaction. | to study the presence of transforming sequence bgl ii n of hsv-2 in cervical cancer tissues, we developed the nested polymerase chain reaction (pcr) for detecting such a sequence in paraffin-embedded cervical tissue sections. samples derived from 46 patients with premalignant and malignant lesions were tested. the sequence was found in 20-25% of total cases tested but not observed in any of the normal healthy controls. this study also indicates that for the detection of hsv-2 bgl ii n sequence i ... | 1994 | 7700264 |
| [genital herpes simplex virus infection among men screened for genital papillomavirus]. | our aim was to assess the frequency of herpetic genital infection (hsv) among men attending a human papillomavirus (hpv) screening centre. clinical screening of a herpetic lesion was completed with biological detection of hsv by cell culture and by polymerase chain reaction (pcr). we also evaluated the role of the male viral factor on the female partners. | 1994 | 7702261 |
| [activation of viral reproduction in a mixed infection with human immunodeficiency virus and herpes viruses]. | mutual activation of reproduction of type 1 hiv and herpes simplex types 1 and 2 viruses (hsv) was observed in simultaneous infection of continuous t-cellular lymphoblastoid lines (cem, 119, hut-78, mt-4, jurkat-tat) and u-937 monocytic line. syncytium formation and cytodestructive pattern of reproduction of viruses of both families in these cell lines necessitated the use of enzyme immunoassay (eia) to detect the antigens of these viruses in order to assess the level of reproduction. the concen ... | 1994 | 7716908 |
| [selection of primers and their use in the polymerase chain reaction for diagnosing infections caused by herpes simplex virus and cytomegalovirus]. | the authors present the results of studies on the construction of highly sensitive primers for rapid diagnosis of infections caused by herpes simplex virus (hsv) and cytomegalovirus. an oligonucleotide primer to hsv-1 is described, which permits detection of hsv-1 dna in infected cultures and in clinical material in dilution 10(-7), whereas the "universal" primer constructed on the basis of published data detects both hsv-1 and hsv-2 only in dilution 10(-4). study of the clinical material in pol ... | 1994 | 7716914 |
| [laboratory criteria for assessing the safety of candidate strains for live vaccines against tick-borne encephalitis]. | the authors present the results of studies on the construction of highly sensitive primers for rapid diagnosis of infections caused by herpes simplex virus (hsv) and cytomegalovirus. an oligonucleotide primer to hsv-1 is described, which permits detection of hsv-1 dna in infected cultures and in clinical material in dilution 10(-7), whereas the "universal" primer constructed on the basis of published data detects both hsv-1 and hsv-2 only in dilution 10(-4). study of the clinical material in pol ... | 1994 | 7716915 |
| immunotherapy with low-dose interleukin-2 and anti-transforming growth factor-beta antibody in a murine tumor model. | the purpose of the present study was to evaluate the therapeutic efficacy of low-dose interleukin-2 (il-2) alone or together with antibody against transforming growth factor-beta (tgf-beta) in a herpes simplex virus type 2-transformed (h238) fibrosarcoma model. balb/c mice were inoculated subcutaneously (s.c.) with 5 x 10(5) h238 tumor cells in one or both hind thighs and treated with il-2, anti-tgf-beta, or a combination of both agents. nontreated tumor-bearing and normal animals served as cont ... | 1994 | 7719379 |
| typing and strain differentiation of clinical herpes simplex virus type 1 and 2 isolates by polymerase chain reaction and subsequent restriction fragment length polymorphism analysis. | in the present study, different combinations of primers were investigated for pcr amplification and typing of clinical herpes simplex virus type 1 (hsv-1) (n = 22) and herpes simplex virus type 2 (hsv-2) (n = 12) isolates. intratypic strain differentiation was performed by restriction fragment length polymorphism (rflp) analysis of different pcr amplified hsv genome regions. enzymatic amplification of hsv dna from all the clinical isolates could be achieved using primer combinations dnap5/dnap3- ... | 1994 | 7727898 |
| sp-303, an antiviral oligomeric proanthocyanidin from the latex of croton lechleri (sangre de drago). | sp-303, a large proanthocyanidin oligomer isolated from the latex of the plant species croton lechleri (eupborbiaceae) has demonstrated broad activity against a variety of dna and rna viruses. in cell culture, sp-303 exhibits potent activity against isolates and laboratory strains of respiratory syncytial virus (rsv), influenza a virus (flu-a) and parainfluenza virus (piv). parallel assays of sp-303 and ribavirin showed comparable activity against these viruses. sp-303 also exhibits significant ... | 1994 | 23195881 |
| necrotizing funisitis and herpes simplex infection of placental and decidual tissues: study of four cases. | necrotizing funisitis (nf) is a macroscopically and microscopically distinctive pattern of umbilical cord inflammation recently heralded as presumptive of congenital syphilis. four nonsyphilitic cases are presented in which herpes simplex virus (hsv) 2 antigen was demonstrated in the placenta by immunohistochemistry. the clinicopathologic findings in one case, including hsv 2 antigen in amniotic epithelium, subamniotic chorion, and wharton jelly, indicate that nf was caused by chronic ascending ... | 1994 | 8026831 |
| follow-up of pregnant women at high risk of transmitting herpes simplex virus. | prospects for neonatal herpes transmission were studied in a group of pregnant chilean women at high herpetic risk--including 59 with a history of genital herpes, 11 with a first genital herpes episode during the observed pregnancy, and 16 whose sexual partners had a history of genital herpes. each woman completed a survey questionnaire, provided serologic samples for detection of herpes simplex virus (hsv), and provided periodic samples taken with cotton swabs for hsv isolation. the 86 women wh ... | 1994 | 8069336 |
| herpes simplex virus infection of the fetus and newborn. | although infrequent, untreated neonatal herpes results in death in half the cases and neurologic sequelae in three quarters of the survivors. neonatal infection is usually acquired from maternal genital herpes, which is asymptomatic or unrecognized in 60% to 80% of women. the greatest risk of neonatal infection occurs when the mother has primary genital herpes involving the cervix at delivery, and the infant is premature and delivered with instrumentation (eg, scalp electrodes). more than 80% of ... | 1994 | 8015861 |
| recent advances in genital herpes. | the majority of transmissions occur as a consequence of inapparent infection in the source contact, as asymptomatic viral excretion in individuals with recurrent infection. the development of type specific serological assays has allowed for a more accurate determination of herpes simplex virus (hsv) 2 prevalence and has revealed that the prevalence of hsv 2 in sexually transmitted disease clinic attenders varies from 8%-83%, in female prostitutes from 75%-96% and in antenatal clinic attenders fr ... | 1995 | 8849193 |
| the hsv-2 la-1 oncoprotein is a member of a novel family of serine threonine receptor kinases. | the large subunit of the herpes simplex virus type 2 ribonucleotide reductase (rr) (icp10) is a chimera consisting of a serine/threonine (ser/thr) protein kinase (pk) domain preceded by a transmembrane (tm) segment at the amino terminus (la-i oncoprotein) and the rr domain at the carboxy terminus. human cells transformed by the la-i oncogene constitutively express the oncoprotein on the cell surface and internalize it by the endocytic pathway as determined by immunogold staining and electron mic ... | 1995 | 21552868 |
| herpesvirus papio 2, an sa8-like alpha-herpesvirus of baboons. | several sa8 isolates obtained from baboons were compared to the prototype sa8 herpesvirus of african green monkeys. sds-page and restriction enzyme analyses revealed definite differences between green monkey and baboon isolates. dna and amino acid sequences of the gb, gd and gj glycoprotein genes exhibited substantial differences in variable regions. for the gb and gd, the amount of amino acid substitutions between sa8 and the baboon viruses was comparable to levels observed between analogous ge ... | 1995 | 7733825 |
| [molecular differential diagnosis of herpes virus using common primer pairs: detection of hsv-1, hsv-2, vzv and cmv by the pcr]. | differential diagnosis of herpes virus species is often required in various clinical courses, such as transplantation and immunocompromised patients. pairs of common primers which enabled sufficient amplifying and differential diagnosis of herpes virus species were designed and nested pcr using the common primer pairs was conducted to evaluate for its clinical application. the pcr was shown to provide amplification and differential diagnosis of hsv-1, hsv-2, vzv and cmv. the results of the pcr w ... | 1995 | 7739118 |
| recurrence rates of genital herpes. | 1995 | 7741383 | |
| inhibition of herpes simplex virus in culture by oligonucleotides composed entirely of deoxyguanosine and thymidine. | oligodeoxynucleotides (odns) composed entirely of deoxyguanosine and thymidine, but not specifically designed to act as antisense agents, were able to significantly inhibit herpes simplex virus growth in acute infection assay systems. the guanosine/thymidine (gt) odns which demonstrated this antiviral activity contained either natural phosphodiester (po) or phosphorothioate (ps) modified internucleoside linkages. in all experiments, the antiviral activity of the odns was enhanced when the backbo ... | 1995 | 7741520 |
| a recombinant glycoprotein vaccine for herpes simplex virus type 2: safety and immunogenicity [corrected]. | to evaluate the safety and immunogenicity of a recombinant glycoprotein vaccine for herpes simplex virus type 2 (hsv-2), which contains glycoproteins gd2 and gb2 combined with the novel mf59 adjuvant emulsion, in hsv-2-seronegative persons. | 1995 | 7755223 |
| neonatal herpes simplex type 2 encephalitis: its appearances on ultrasound and ct. | we report the ultrasound findings in a 28-day-old infant with encephalitis due to herpes simplex type 2. to date less than ten such cases have appeared in the world literature. asymmetric ventricular dilatation was seen sonographically with widespread parenchymal hyperechoic areas which corresponded to areas of low attenuation on ct. cranial ultrasound has a distinctive appearance which, if recognised, and suggested, can lead to an early definitive diagnosis. | 1995 | 7761173 |
| neonatal herpes simplex virus infection presenting as fulminant liver failure. | 1995 | 7761194 | |
| effect of imiquimod as an adjuvant for immunotherapy of genital hsv in guinea-pigs. | imiquimod, an immunomodulator which upregulates cell-mediated immune responses, was evaluated as an adjuvant for immunotherapy of recurrent genital herpes simplex virus (hsv) infection in guinea-pigs. in two experiments at separate research centres, animals were immunized with hsv glycoprotein and either placebo, 1 or 5 days of imiquimod, or complete freund's adjuvant, 14 and 35 days after genital hsv-2 infection. recurrent lesion days were then evaluated from days 15-91. in both experiments, im ... | 1995 | 7762282 |
| comparative study of the genetic variability in thymidine kinase and glycoprotein b genes of herpes simplex viruses by the rnase a mismatch cleavage method. | the variability of herpes simplex viruses has been measured using the rnase a mismatch cleavage method in two genes: thymidine kinase and glycoprotein b of both hsv-1 and hsv-2. this technique permitted us to study the variability of the virus with a greater level of resolution than restriction endonuclease analysis. the phylogenetic trees obtained for the different genes allowed us to identify consistent clusters of viruses circulating in the same geographical area. our results showed that thym ... | 1995 | 7762293 |
| [immune system in women with genital inflammatory diseases caused by chlamydia and virus infection]. | immunologic studies were carried out in 29 women with clinical manifestations of chronic inflammations of the internal genitals. dot-hybridization with biotin-labeled dna probes revealed chlamydial dna in the cervical cells of 14 women, herpes simplex type 2 and/or cytomegaloviral dna in 15 women. cell-mediated immunity was assessed using monoclonal antibodies cd3, cd4, cd8, and cd19 and by facscan flow cytofluorometer (becton dickinson). immunoglobulins were measured by nephelometry with abbott ... | 1995 | 7762752 |
| are human herpes viruses or measles virus associated with esophageal achalasia? | in order to test the hypothesis that esophageal achalasia may be due to neurotropic viral damage to the esophageal myenteric plexus, esophageal tissue with or without achalasia was analyzed by polymerase chain reaction for the presence of human herpes virus dna or measles virus rna. the dna and rna were extracted from the esophageal muscle of 12 patients with achalasia and six patients with upper esophageal carcinoma. peripheral blood mononuclear cells from eight adult volunteers and two samples ... | 1995 | 7720482 |
| comparative studies of types 1 and 2 herpes simplex virus infection of cultured normal keratinocytes. | to investigate the differences in biological properties, multiplication patterns, and cytopathic effects between type 1 and type 2 herpes simplex virus (hsv) through the replication of hsv in cultured normal human keratinocytes. | 1995 | 7706526 |
| a controlled seroprevalence survey of primate handlers for evidence of asymptomatic herpes b virus infection. | herpes b virus (bv) is a common cause of recurring mucocutaneous infections in monkeys of the genus macaca. like its human counterpart, herpes simplex virus (hsv), bv establishes lifelong latency and can be reactivated from infected monkeys symptomatically or asymptomatically. incidental infection of humans handling bv-shedding monkeys can result in fatal meningoencephalitis. to determine whether humans exposed to infected monkeys can acquire asymptomatic bv infections, 480 subjects were evaluat ... | 1995 | 7706783 |
| analysis of the promoter and cis-acting elements regulating expression of herpes simplex virus type 2 latency-associated transcripts. | in latently infected neurons, herpes simplex virus type 2 (hsv-2) expresses one abundant family of transcripts, the latency-associated transcripts (lats). we demonstrate here that the sequence lying about 700 bp upstream of the 5' end of the hsv-2 major lat acts as a very strong promoter in transient expression assays in both neuronal and nonneuronal cells. transcription starts about 27 to 32 bp downstream of a functional tata box. the proximal fragment from -102 to +34 includes the basal promot ... | 1995 | 7707511 |
| in vivo antiherpesvirus activity of n-7-substituted acyclic nucleoside analog 2-amino-7-[(1,3-dihydroxy-2-propoxy)methyl]purine. | the efficacy of 2-amino-7-[(1,3-dihydroxy-2-propoxy)methyl]purine (s2242) was evaluated in several animal models for herpesvirus infections. compound s2242 was more effective than acyclovir (i) when administered subcutaneously in a model for herpes simplex virus type 1 (hsv-1)-induced mortality in immunocompetent mice and (ii) when applied topically to hairless (hr/hr) mice that had been infected intracutaneously with hsv-2. in scid (severe combined immune deficient) mice that had been infected ... | 1995 | 7695329 |
| design, synthesis, and structure-activity relationship of novel dinucleotide analogs as agents against herpes and human immunodeficiency viruses. | a new acyclic nucleoside phosphonate (13) containing an adenine moiety was synthesized, which acted as an excellent inhibitor of calf mucosal adenosine deaminase. this inhibitory property allows it to exert great synergistic effect on certain antiviral agents (e.g., ara-a, 37). phosphonate 13 was not phosphorylated by the bovine brain guanylate kinase nor by 5-phosphoribosyl 1-pyrophosphate synthetase. syntheses of biologically active nucleotide phosphonate 40 and its phosphonoamidate derivative ... | 1995 | 7473592 |
| role of the virion host shutoff (vhs) of herpes simplex virus type 1 in latency and pathogenesis. | the herpes simplex virus type 1 (hsv-1) ul41 gene product, virion host shutoff (vhs), has homologs among five alphaherpesviruses (hsv-1, hsv-2, pseudorabies virus, varicella-zoster virus, and equine herpesvirus 1), suggesting a role for this protein in neurotropism. a mutant virus, termed ul41nhb, which carries a nonsense linker in the ul41 open reading frame at amino acid position 238 was generated. ul41nhb and a marker-rescued virus, ul41nhb-r, were characterized in vitro and tested for their ... | 1995 | 7474089 |
| herpes simplex virus-2 may increase susceptibility of the sexual transmission of hepatitis c. | antibodies against herpes simplex viruses-1 and -2, cytomegalovirus, and syphilis were determined in six heterosexual couples with strong indications of having sexually transmitted hepatitis c virus infection and in 17 other heterosexual couples in which one partner was hepatitis c virus viremic (source partner), but the other had remained hepatitis c virus uninfected (exposed partner). | 1995 | 7482102 |
| increased genital shedding of herpes simplex virus type 2 in hiv-seropositive women. | to compare the prevalence of genital herpes simplex virus type 2 (hsv-2) shedding in human immunodeficiency virus (hiv)-seropositive women and hiv-seronegative women. | 1995 | 7486467 |
| mode of action of (r)-9-[4-hydroxy-2-(hydroxymethyl)butyl]guanine against herpesviruses. | the activity, metabolism, and mode of action of (r)-9-[4-hydroxy-2-(hydroxymethyl)butyl]guanine (h2g) against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and varicella-zoster virus (vzv) were studied. compared to acyclovir (acv), h2g has superior activity against vzv (50% inhibitory concentration of 2.3 microm) and epstein-barr virus (50% inhibitory concentration of 0.9 microm), comparable activity against hsv-1, and weaker activity against hsv-2. the antiviral effect on hsv-1 showed ... | 1995 | 7486922 |
| suppression of infectious virus spread to the liver by foscarnet following lethal infection of acyclovir-resistant herpes simplex virus type 2 in mice. | patients with the acquired immune deficiency syndrome (aids) occasionally develop hepatitis, pneumonia or esophagitis due to herpes simplex virus type 2 (hsv-2) infection. hsv hepatitis is a rare but serious complication in liver transplantation. acyclovir-resistant hsv strains may emerge in immunocompromised patients. following intraperitoneal inoculation, hsv-2 induces necrotizing hepatitis in mice. we studied the virus spread and mortality following intraperitoneal inoculation of hsv-2 rk (an ... | 1995 | 7486949 |
| the us3 protein kinase of herpes simplex virus type 2 is associated with phosphorylation of the ul12 alkaline nuclease in vitro. | herpes simplex virus type 2 (hsv-2) gene us3 encodes a serine-threonine protein kinase. we previously described the isolation of a us3-inactivated mutant which is able to replicate in vero cells but not in murine macrophages. to learn more about the biological role of the us3 protein kinase, we have sought to identify the target proteins of the enzyme. studies of in vitro phosphorylation with extracts of infected cells demonstrate that the us3 protein kinase is involved in phosphorylation of the ... | 1995 | 7487495 |
| immunization with recombinant varicella-zoster virus expressing herpes simplex virus type 2 glycoprotein d reduces the severity of genital herpes in guinea pigs. | varicella-zoster virus (vzv) is an attractive candidate for a live-virus vector for the delivery of foreign antigens. the oka vaccine strain of vzv is safe and effective in humans, and recombinant oka vzv (roka) can be generated by transfecting cells with a set of overlapping cosmid dnas. by this method, the herpes simplex virus type 2 (hsv-2) glycoprotein d (gd2) gene was inserted into an intergenic site in the unique short region of the oka vzv genome. expression of gd2 in cells infected with ... | 1995 | 7494331 |
| roles of different t-cell subsets in control of herpes simplex virus infection determined by using t-cell-deficient mouse-models. | herpes simplex virus infection of the scarified dermis results in infection of the nervous system and, subsequently, a cutaneous lesion in the innervated dermatome. we compared the pathogenesis of such zosteriform lesions in mice lacking or severely depleted of cd4+ or cd8+ t cells because of targeted gene disruption. mice without cd4+ cells showed markedly increased susceptibility, whereas beta 2 microglobulin knockout mice lacking cd8+ t cells were as resistant to challenge as were immunocompe ... | 1995 | 7494346 |
| antiviral activity of trichothecene mycotoxins (deoxynivalenol, fusarenon-x, and nivalenol) against herpes simplex virus types 1 and 2. | the effect of trichothecene mycotoxins, deoxynivalenol (don), fusarenon-x (fx) and nivalenol (niv), on plaque formation of herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) in hep-2 cells was examined. the 50% effective concentrations (ec50) of don, fx, and niv for hsv-1 plaque formation were 160, 56, and 120 ng/ml, respectively. those for hsv-2 plaque formation were 94, 26, and 50 ng/ml, respectively. these three mycotoxins showed about 2-fold higher selectivity to hsv-2 than to hsv-1. plaqu ... | 1995 | 7494505 |
| improved methods for determination of rotational symmetries in macromolecules. | rotational symmetries of macromolecules are most clearly perceived in the en face projection and may be assessed by inspection of rotational power spectra calculated from electron micrographs of individual particles. however, if the symmetry is not contrasted strongly, this procedure may be inconclusive since the relevant peak may not be convincingly higher than other spectral components. to some extent, this is a sampling problem since the number of repeating elements involved is usually small. ... | 1995 | 7502382 |