Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| major glycoproteins in carp cns myelin: homology to p0 protein with hnk-1/l2 carbohydrate epitope. | we studied the myelin protein profiles of carp from a phylogenetic point of view. the carp central nerve myelin contained two reactive bands, 28 and 25 kda, demonstrated with anti-bovine p0 antibody. their molecular weights are slightly different from those of two positive bands found in carp peripheral myelin. the n-terminal amino acid sequences of these four positive bands were identical to one another and showed high homology with those of mammalian p0 protein, suggesting that carp central my ... | 1993 | 7693109 |
| cystine/glutamate antiporter expression in retinal müller glial cells: implications for dl-alpha-aminoadipate toxicity. | a cytotoxicity of glutamate or related amino acids (10 mm) mediated by a cystine/glutamate antiporter (system xc) has recently been demonstrated in n18 neuroblastoma-rat retina hybrid (n18re105) cells and c6 glioma cells. the antiporter usually transports glutamate outside and cystine inside, thereby maintaining cellular concentrations of glutathione. high concentrations of glutamate inhibit cystine uptake and lead to depletion of cellular levels of glutathione. among related amino acids, dl-alp ... | 1993 | 7906874 |
| studies on the toxic and immunosuppressive effects of cadmium on the common carp. | in the last couple of decades, research on cadmium extended from its toxicologic aspects to its role in environmental pollution. the toxicity of cadmium to different species of fish varies widely. the cadmium sensitivity of batches of common carp, the most important cultured fish species of hungary, was studied. lethality, histopathologic changes and effect of cadmium on the antibody response, lysozyme level and microbicidal capacity were monitored. wide variation was found in individual sensiti ... | 1993 | 8017244 |
| the occurrence of sphaerospora renicola k-stages in the choroidal rete mirabile of the common carp. | it is known from earlier studies that the so-called k-protozoa, the late presporogonic stages of sphaerospora renicola, a parasite causing renal sphaerosporosis in common carp (cyprinus carpio), accumulate in the swimbladder wall and give rise to the disease called myxosporean swimbladder inflammation. the present study indicates that in the case of intensive infection these parasites also occur in the rete mirabile of the choroid of the eye, causing haemorrhages and necrosis. the results sugges ... | 1993 | 8314174 |
| purification and properties of an indian major carp (cirrhinus mrigala, ham.) pituitary thyrotropin. | thyroid stimulating hormone (tsh) was isolated and purified from the pituitaries of indian major carp, cirrhinus mrigala, with the help of a sensitive in vitro bioassay system, based on incubating isolated thyroid follicles from murrel, channa gachua. in this assay, addition of test material containing tsh increased thyroxine (t4) release into the medium which was then measured by radioimmunoassay (ria). tsh activity was eluted as one major peak on sephadex g-100 gel filtration (peak i), which a ... | 1993 | 8319876 |
| expansion of genes that encode mhc class i molecules in cyprinid fishes. | we have analyzed the genomic genes and the mrna that encode mhc class i molecules in cyprinid fishes, namely, the ginbuna crucian carp (carassius auratus langsdorfii) and the carp (cyprinus carpio). the presence of several distinct class i genes was revealed in the genome of cyprinid fishes, and distinct class i genes that are expressed in carp cells were identified. the sequences of the alpha 3 domain of these class i genes are relatively well conserved, whereas the sequences of the respective ... | 1993 | 8326125 |
| effects of inoculum route and dose on the immune response of common carp, cyprinus carpio to the blood parasite, trypanoplasma borreli. | carp were inoculated intramuscularly (im) or intraperitoneally (ip) with different doses of live trypanoplasma borreli. prepatent period was shortened by using the im route and by increasing the dose. haematocrit was reduced following im inoculation with 9.5 x 10(5) parasites. most fish recovered between 8 and 13 weeks post-inoculation (wpi). neither parasitaemia nor anaemia was detected in any recovered fish following homologous challenge. an enzyme-linked immunosorbent assay was developed to q ... | 1993 | 8333145 |
| regulation of expression of transgenes in developing fish. | the transcriptional regulatory elements of the beta-actin gene of carp (cyprinus carpio) have been examined in zebrafish and goldfish harbouring transgenes. the high sequence conservation of the putative regulatory elements in the beta-actin genes of animals suggested that their function would be conserved, so that transgenic constructs with the same transcriptional control elements would promote similar levels of transgene expression in different species of transgenic animals. to test this assu ... | 1993 | 8353534 |
| expression and inheritance of rsvltr-rtgh1 complementary dna in the transgenic common carp, cyprinus carpio. | transgenic common carp, cyprinus carpio, possessing the long terminal repeat (ltr) sequence of avian rous sarcoma virus (rsv) fused to the rainbow trout (rt) growth hormone (gh1) complementary dna (cdna) were produced by microinjection. initial studies showed that the transgenic common carp transmitted the foreign dna to a significant fraction of their progeny in three of four crosses of transgenic males with control females. these progeny grew 20 to 40% faster than their nontransgenic full sibl ... | 1993 | 8364692 |
| efficiency of introns from various origins in fish cells. | several vectors containing (1) regulatory regions from rous sarcoma virus (rsv), human cytomegalovirus (cmv), and herpes simplex thymidine kinase (tk); (2) introns from early or late sv40 genes and from trout growth hormone gene (tgh); (3) chloramphenicol acetyltransferase gene (cat); and (4) transcription terminators from sv40 were transfected into carp epc cells, salmon chse cells, tilapia to2 cells, quail qt6 cells, and hamster cho cells. cat activity was measured in extracts from several cel ... | 1993 | 8395951 |
| recombinant carp (cyprinus carpio) growth hormone: expression, purification, and determination of biological activity in vitro and in vivo. | carp growth hormone (cgh) cdna (koren et al., 1989) was cloned under the control of lambda-phage plol promoter and lambda cll ribosomal binding site into pbr322 plasmid to enable its expression in escherichia coli a1645 that produces constitutively the thermolabile lambda repressor c1857. temperature shift to 42 degrees abolished the repression, resulting in a high level of cgh expression. the bacterially expressed cgh protein, contained within the refractile body pellet, was solubilized in 4.5 ... | 1993 | 8428649 |
| cloning and sequence analysis of cdnas encoding the mhc class ii beta chain in atlantic salmon (salmo salar). | atlantic salmon (salmo salar) cdnas encoding the major histocompatibility complex (mhc-sasa) class ii beta chain were isolated from a leucocyte library by a polymerase chain reaction (pcr) approach. three different cdnas (c144, c22, and c157) encoding the entire mature beta chain have been analyzed. clone c144 differs from clone c157 in 12.6% of the nucleotides in the beta 1-encoding region. the corresponding differences between clones c144 and c22, and clones c22 and c157, are 10.3% and 5.2%, r ... | 1993 | 8436418 |
| plasmodium falciparum: the immune response in rabbits to the clustered asparagine-rich protein (carp) after immunization in freund's adjuvant or immunostimulating complexes (iscoms). | the plasmodium falciparum clustered asparagine-rich protein (carp) is a merozoite-associated antigen which contains approximately 30% asparagine. analysis of the dna sequences located 5' of the cloned 1.4-kb carp gene in the p. falciparum genome suggests that this gene fragment may encode the complete carp and that the gene product is a protein of m(r) 50,000. to analyze the immunogenicity of carp, the gene was expressed as a fusion protein with staphylococcal protein a (spa-carp). immunization ... | 1993 | 8454022 |
| distribution of non-class-iii beta-tubulin isoforms in neuronal and non-neuronal cells. | beta-tubulin isoforms in brain tissues and in cell lines were analyzed by high-resolution isoelectric focusing in combination with monoclonal antibodies. post-translational modifications of brain non-class-iii beta-tubulin isoforms were found in phylogenetically distant species ranging from pig to carp. less extensive modifications were also observed in neuro-2a, hela and 3t3 cells, where most acidic isoforms were glutamylated, while the basic, most abundant isoforms were not. the data suggest p ... | 1993 | 8462682 |
| characterization of beta 2-microglobulin transcripts from two teleost species. | using degenerate primers based on published beta 2-microglobulin sequences we were able to obtain an expected 111 base pairs (bp) polymerase chain reaction (pcr) fragment from tilapia genomic dna. the sequence of this fragment showed a high degree of similarity to mouse beta 2-microglobulin at the protein level. we used these primers in an "anchored pcr" to obtain a 213 bp pcr fragment from a carp cdna library. this was then used to clone a full-length beta 2-microglobulin cdna from carp. the ca ... | 1993 | 8462991 |
| an invertebrate calcium-binding protein of the calbindin subfamily: protein structure, genomic organization, and expression pattern of the calbindin-32 gene of drosophila. | antisera against vertebrate calcium-binding proteins cross-react with drosophila nervous and muscle tissue. we have used an antiserum against carp parvalbumin to isolate from a drosophila head cdna library immunopositive expression clones. tissue in situ hybridization identified a clone that labeled specific neurons and muscles similar to the parvalbumin-like immunohistochemical staining pattern. five independent cdnas derive from an mrna whose open reading frame codes for a 310 amino acid polyp ... | 1993 | 8478695 |
| the deduced amino acid sequence of human carbonic anhydrase-related protein (carp) is 98% identical to the mouse homologue. | a recently reported mrna, encoding 'carbonic anhydrase-related polypeptide' (carp) from the purkinje cells of mouse cerebellum, was shown to have a 30-40% deduced amino acid sequence identity with the carbonic anhydrases (ca) of mammals. in order to compare the mouse and human carp sequences, we used the polymerase chain reaction (pcr) to amplify human carp sequences from several cdna libraries (salivary gland, testis and placenta). the sequence has an 89.3% sequence identity with mouse carp at ... | 1993 | 8482548 |
| tempo and mode of mitochondrial dna evolution in vertebrates at the amino acid sequence level: rapid evolution in warm-blooded vertebrates. | by using complete sequence data of mitochondrial dnas, three markov models (dayhoff, proportional, and poisson models) for amino acid substitutions during evolution were applied in maximum likelihood analyses of mitochondrially encoded proteins to estimate a phylogenetic tree depicting human, cow, whale, and murids (mouse and rat), with chicken, frog, and carp as outgroups. a cow/whale clade was confirmed with a more than 99.8% confidence level by any of the three models, but the branching order ... | 1993 | 8483165 |
| isolation and primary structure of the three major forms of granulin-like peptides from hematopoietic tissues of a teleost fish (cyprinus carpio). | granulins are cysteine-rich polypeptides purified from human and rat hematopoietic cells and structurally related to the epithelin family of growth modulatory factors. a prototypic form of granulin was isolated from the hematopoietic organs of teleost fish (belcourt, d., and bennett, h.p.j. (1988) j. cell biol. 107, 629 (abstr.)). this study reports the structure of three granulins purified from the spleen and head kidney of the carp (cyprinus carpio). ion-spray mass spectrometric analysis of gr ... | 1993 | 8486624 |
| [the population level and distribution of ectoparasites in carp]. | the abundance and the distribution of dactylogyrus extensus and ichthyophthirius multifiliis on gills of two-years old carps have been investigated. it has been established that at least there are five groups, which differs from each other on the asymmetry of infection. | 1993 | 8488057 |
| presence of growth hormone receptors in carp liver and intestine. | membranes were prepared from tissues of the carp ctenopharynogodon idellus including liver, kidney, intestine, adipose tissue, ovary, gill, heart, muscle and spleen. the carp liver and intestine membranes bound 125i-labeled bovine growth hormone and the binding could be displaced by cold bovine growth hormone. no changes in the ability to bind 125i-labeled bovine growth hormone occurred after treatment of the carp liver membrane with dnase, rnase, alpha-amylase and beta-glucosidase, suggesting t ... | 1993 | 8490577 |
| intestinal, segmented, filamentous bacteria in a wide range of vertebrate species. | segmented, filamentous bacteria (sfbs) form a group of bacteria with similar morphology and are identified on the basis of their morphology only. the relationships of these organisms are unclear as the application of formal taxonomic criteria is impossible currently due to the lack of an in vitro technique to culture sfbs. the intestine of laboratory animals such as mice, rats, chickens, dogs, cats and pigs is known to harbour sfbs. to see whether this extends to other animal species, intestines ... | 1993 | 8501895 |
| determination of ichthiomycin concentration by biological test with cyprinus carpio l. | we have found out that one-year-old carps are extremely sensitive to ichthiomycin in concentrations between 25-125 micrograms/dm3. this fact permitted the use of biological methods for determination of ichthiomycin concentrations in cultural medium of streptomyces levoris 1107 or crude preparations of the antibiotic. | 1993 | 8511994 |
| gaba- and glycine-immunoreactive neurons in the spinal cord of the carp, cyprinus carpio. | gamma-aminobutyric acid (gaba) and glycine are the two main inhibitory transmitter amino acids in the central nervous system of vertebrates. the distribution of cells containing gaba and glycine in the carp spinal cord was examined by using specific antisera raised against the two amino acids conjugated to bovine serum albumin. the immunoreaction on serial paraffin sections was visualized by a streptavidin-biotin method. both antisera gave highly specific labelings of cells. at least three types ... | 1993 | 8514921 |
| [high molecular weight forms of aminoacyl-trna synthetases in animals. ii. high molecular weight aminoacyl-trna synthetase complexes in lower animals]. | high-molecular-weight aminoacyl-trna synthetase complexes have been found in hen, frog and carp liver cytosol as well as in cytosol from snails and paramecium. the complexes from vertebrates and snails contained 13-15 aminoacyl-trna synthetases, revealed mass of about 4,000 kda and sedimentation constant about 19 s. the complex from paramecium containing 11 aminoacyl-trna synthetases, revealed a mass of about 1,600 kda and sedimentation constant 15 s. the results of the present study support the ... | 1993 | 8534161 |
| [the protein level in bothriocephalus acheilognathi cestodes and their hosts, carp fingerlings, in experimental feeding and starving of the fish]. | 1993 | 8152846 | |
| a case-control study of oral cancer in beijing, people's republic of china. associations with nutrient intakes, foods and food groups. | a case-control study of oral cancer was conducted in beijing, people's republic of china to examine the association between dietary nutrient intake and risk of oral cancer, both in terms of estimated intake of nutrients and micro-nutrients, and in terms of specific foods and food groups. the study was hospital-based and controls were hospital in-patients matched for age and sex with the cases. the response rate for cases and controls was 100% and 404 case/control pairs were interviewed. the resu ... | 1993 | 8180577 |
| comparative studies on fish and bird insulin receptors. | the comparative studies were performed on insulin receptors of erythrocytes and liver plasma membranes in fish (tench and carp) and bird (duck). the scatchard plots indicated the presence of two pools of binding sites both in fish and duck. these pools show inter-species differences in binding ability and the number of receptors. specific binding of insulin and the binding affinity are higher in duck than in fish. | 1993 | 8212960 |
| estimation of the genetic variation in complement activity of common carp (cyprinus carpio l.). | the complement status of hybrid, laboratory raised carp was determined by an in vitro approach of the alternate complement activity (ach50) and total haemolytic activity (ch50), and by measurement of serum c3 levels. the lysis of target sheep red blood cells (rbc) in the haemolytic assay for ch50 activity depended, amongst others, on the haemolysin concentration in the assay. rocket electrophoresis showed a mean serum c3 concentration of 0.95 mg ml-1. the variation for both ach50 and ch50 haemol ... | 1993 | 8236805 |
| [localization and activity of several enzymes in tissues of bothriocephalus acheilognathi and khawia sinensis tapeworms parasitizing carp]. | in this study, an attempt has been made to compare the most common species of tapeworms in carp: non-segmentary khawia sinensis and segmentary bothriocephalus acheilognathi on the basis of histoenzymatic examination of 8 enzymes, i.e. alkaline phosphatase, acid phosphatase, adenosine triphosphatase, nonspecific esterase alfa, succinic dehydrogenase, lactate dehydrogenase, leucine aminopeptidase and gamma-glutamylo transferase. | 1993 | 8237009 |
| multiphasic allometry. | a multiphasic linear model is presented that describes the allometric relation between body components and permits a smooth transition from one linear segment to the next. three applications of a diphasic linear model to allometric growth problems were used to illustrate the model. the data sets were selected because a breakpoint exists in the allometric relation. relations between growth of the alimentary tract and of empty body weight in rabbits, growth of length and of body weight in larval-c ... | 1993 | 8244622 |
| the expression of rat gap-43 cdna in transgenic carp. | to understand more about growth-associated protein-43 (gap-43), we produced transgenic carp by introduction of a rat gap-43 cdna linked to the rous sarcoma virus-long terminal repeat into fertilized eggs. of 180 eggs microinjected with exogenous gene, 59 embryos hatched and 4 fish were found to contain the exogenous gene sequences in the genomic dna. from a mature female transgenic carp, parthenogenetically, 126 progeny were derived and 52 of them survived for more than 90 days. the exogenous ge ... | 1993 | 8257558 |
| antibody dependent haemolysin, complement and opsonin in sera of a major carp, cirrhina mrigala and catfish, clarias batrachus and heteropneustes fossilis. | the present communication is a continuation of our earlier study on the natural serum haemagglutinin/lectins of cirrhina mrigala, clarias batrachus and heteropneustes fossilis. sera of cirrhina mrigala, belonging to the major carp family, could not only agglutinate heterologous rabbit erythrocytes, but also lyse them spontaneously. this lysis of rabbit rbc by cirrhina mrigala sera was calcium ion dependent and heat sensitive, indicating thereby that the haemolysis was mediated by the fish serum ... | 1993 | 8281746 |
| localized leucocyte response to ichthyophthirius multifiliis establishment in immune carp cyprinus carpio l. | o-group carp (cyprinus carpio) which had been immunized against ichthyophthirius multifiliis by controlled infections were challenged by topical application of theronts to the caudal fin. the parasites which established were examined ultrastructurally, and host leucocyte responses were compared with those observed in primary infections. in the primary exposure group eosinophils and (to a lesser extent) basophils were the predominant cells infiltrating infection sites. in contrast, parasite devel ... | 1993 | 8291210 |
| immunoreactive atrial natriuretic peptides in the oocyte. | 1. the presence and partial characterization of immunoreactive anp (iranp) in eggs were investigated using high performance liquid chromatography (hplc), immunohistochemistry and northern-blot hybridization in mammalian and nonmammalian vertebrates. 2. serial dilution curves of egg extracts from frogs and freshwater teleostean fishes (silver crusian carp and loach) were parallel to the standard curve of atriopeptin iii. rat oocytes also contained iranp (25-30 pg/egg). 3. the hplc profile of iran ... | 1993 | 8095876 |
| purification and characterization of urotensin ii from the brain of a teleost (trout, oncorhynchus mykiss) and an elasmobranch (skate, raja rhina). | peptides related to urotensin ii have been isolated in pure form from an extract of whole brain of a teleost, the rainbow trout (oncorhynchus mykiss) and of an elasmobranch, the longnose skate (raja rhina). the primary structure of the trout peptide [gly-gly-asn-ser-glu-cys-phe-trp-lys-tyr-cys-val] is similar to that of urotensin ii peptides isolated from the urophyses of other teleost fish. for example, trout urotensin ii contains only one amino acid substitution (thr4-->ser) compared with urot ... | 1993 | 8138107 |
| molecular endocrinology of oocyte growth and maturation in fish. | pituitary gonadotropins (gths) are of primary importance in triggering oocyte growth and maturation. however, the actions of gths are not direct, but are mediated by the ovarian production of steroidal mediators of oocyte growth (estradiol-17β) and maturation (maturation-inducing hormone, mih; 17α,20β-dihydroxy-4-pregnen-3-one, 17α,20β-dp in salmonid fishes; 17α,20β,21-trihydroxy-4-pregnen-3-one, 20β-s in sciaenid fishes). it is established that production of estradiol-17β and 17α,20β-dp by salm ... | 1993 | 24202455 |
| preparation and comparison of biological properties of recombinant carp (cyprinus carpio) growth hormone and its cys-123 to ala mutant. | carp growth hormone (cgh) cdna, in which cys-123 was mutated to ala, was prepared, transferred to the expression vector, expressed in escherichia coli and the mutant was purified to homogeneity. the mutation only slightly improved yield of the monomeric fraction, indicating that cys-123 is not involved in improper refolding. as compared to cgh, the mutant (cgh-c123a) exhibited lower binding affinity toward homologous liver receptors and lower bioactivity in a 3t3-f442a preadipocyte bioassay desp ... | 1993 | 24202495 |
| cytoarchitecture, fiber connections, and ultrastructure of the nucleus pretectalis superficialis pars magnocellularis (psm) in carp. | the cytoarchitecture, fiber connections, and ultrastructure of the nucleus pretectalis superficialis pars magnocellularis (psm) were studied in cypriniform teleosts (cyprinus carpio). the psm is an oval nucleus in the pretectum. medium-sized cells and synaptic glomeruli are the main components of the nucleus. a lesser number of small cells are also present. most of the medium-sized cells form one or two cell layers on the periphery of the nucleus, and some cells are scattered among synaptic glom ... | 1993 | 8263230 |
| the branching of insulin-like growth factor 1 and insulin: an immunohistochemical analysis during phylogeny. | the co-existence of insulin-like growth factor 1 (igf-1) with the classical islet hormones insulin (ins), glucagon (gluc), somatostatin (som) and pancreatic polypeptide (pp) in the endocrine pancreas of representative species of cyclostomes (myxine glutinosa), cartilaginous fish (raja clavata, squalus acanthias) and bony fish (cottus scorpius, carassius auratus, cyprinus carpio, anguilla anguilla) was studied by the use of monoclonal and polyclonal antisera and the double immunofluorescence tech ... | 1993 | 8265818 |
| differential distribution and response to experimental sexual maturation of two forms of brain gonadotropin-releasing hormone (gnrh) in the european eel, anguilla anguilla. | using specific radioimmunoassays for the two gnrh molecular forms present in the european eel, anguilla anguilla, (mgnrh and cgnrh ii), we compared their distributions in the pituitary and different parts of the brain of female silver eels, as well as the modifications of their levels in experimentally matured female eels (treated with carp pituitary extract). in control eels, mgnrh levels were higher than cgnrh ii levels in the pituitary, olfactory lobes and telencephalon, di- and mesencephalon ... | 1993 | 24202465 |
| trading off safety against food: state dependent habitat choice and foraging in crucian carp. | the influence of hunger level and predation risk on habitat choice and foraging in crucian carp, carassius carassius, were studied in a laboratory experiment. experiments were carried out in aquaria with or without a predator (pike, esox lucius). habitat use and foraging activity of three-fish foraging groups of either fed or hungry crucian carp were studied. fish were allowed to choose between an open (risky) habitat with tubifex worms and a habitat with dense vegetation (safe) without food. ha ... | 1993 | 28314010 |
| brain sensitivity to anoxia in fish as reflected by changes in extracellular k+ activity. | most vertebrates have a very limited tolerance to anoxia. the only exceptions to this rule are found among ectothermic species, notably crucian carp (carassius carassius) and freshwater turtles. it has been assumed that the brains of these species are less sensitive to anoxia than ectothermic brains in general. however, it has not been possible to exclude that anoxia-intolerant species like rainbow trout (oncorhynchus mykiss) die from heart failure rather than brain failure during anoxia, and th ... | 1993 | 8447481 |
| influence of seasonal adaptation on the distribution of cytochemically detectable endogenous calcium in synaptic terminals of the optic tectum of crucian carp (carassius carassius l.). | crucian carps were seasonally adapted to high (23 degrees c) or low (4 degrees c) temperatures and synaptic regions in the optic tectum were analyzed by means of energy-filtering transmission electron microscopy (eftem). the amount of cytochemically detectable endogenous calcium in synaptic clefts was quantified by means of image analysis and the amount of glycogen granules in synaptic regions was assessed. in cold-adapted fish, glycogen is increased, especially in glial cells, while the calcium ... | 1993 | 8270788 |
| dna adduct detection: some applications in monitoring exposure to environmental genotoxic chemicals. | in the assessment of genotoxic risk factors in the environment, the measurement of dna adducts in aquatic organisms and in plants may have considerable implications. using 32p-postlabelling, we have detected dna adducts in the liver of carp (chondrostoma nasus) from the river rhône (france), both downstream and upstream from a polychlorinated biphenyl incineration plant. some of the dna adducts were specific to downstream fish, suggesting a differential pattern of exposure. we have also detected ... | 1993 | 8225509 |
| a coccidian (apicomplexa: eimeriidae) with extracytoplasmally located stages in the kidney tubules of golden carp (carassius auratus gibelio l.) (cyprinidae). | numerous coccidian stages were found in the kidney tubules of the golden carp (carassius auratus gibelio). the merogonial and gamogonial stages were localized extracytoplasmally in the microvillous region of the epithelial cells. the host-parasite interface consisted of i) a large area where the parasite was separated from the host cytoplasm by the parasitophorous vacuole membrane only, and ii) a zone of multiple fusions of the host cell membrane investing the parasite to the neighbouring microv ... | 1993 | 8325562 |
| vimentin persists in the mature glia of fish brain. | adult carp brains were processed for immunohistochemical staining against vimentin and glial fibrillary acidic protein. the shape, density and distribution of immunopositive structures were found to be very similar with both types of antibody. thus, it appears that vimentin, the cytoskeletal protein typical for immature glia in the early phase of ontogenesis in mammals can also be demonstrated in the adult glia of the phylogenetically older fish brain. | 1993 | 8369799 |
| biomonitoring of aquatic systems. | the 32p-postlabelling analysis provides a sensitive means for detecting pollution-related dna adducts in aquatic organisms exposed to environmental carcinogens. however, the following factors need to be taken into consideration during the data interpretation: (1) species-specific, naturally occurring dna modifications (or i-compounds) are found in aquatic organisms at levels which are highly season-dependent; and (2) many aquatic organisms, particularly lower invertebrates, cannot form dna adduc ... | 1993 | 8225508 |
| response of serum calcium to administration of 1,25-dihydroxyvitamin d3 in the freshwater carp cyprinus carpio maintained either in artificial freshwater, calcium-rich freshwater or calcium-deficient freshwater. | freshwater carps (cyprinus carpio) were injected daily intraperitoneally either with vehicle (0.1 ml 95% ethanol/100 gm body wt), or 1,25(oh)2d3 (5 iu/100 gm body wt) and maintained either in artificial-freshwater, calcium-rich freshwater or calcium-deficient freshwater for 15 days. the specimens were sacrificed on day 1, 3, 5, 10 and 15 after initiation of the experiment and serum calcium levels were analysed. (i) artificial freshwater: serum calcium level of 1,25(oh)2d3 treated specimens incre ... | 1993 | 8197882 |
| recent achievements in the chemotherapy of myxosporean infections of fish. | until quite recently, the therapy of fish diseases caused by myxosporeans has been an unsolved problem. fumagillin is the only effective drug which can be successfully applied against several myxosporeoses in the form of prolonged feeding. it has been used with good results against sphaerosporosis of common carp, hoferellosis of goldfish, myxidiosis of eel, and proliferative kidney disease, whirling disease and ceratomyxosis of salmonids. already a 4- to 6-day feeding of medicated diets containi ... | 1993 | 8116501 |
| sensitivities of achatina giant neurones to peptides isolated from mollusca. | sensitivities of the achatina giant neurones to the peptides isolated from mollusca were examined by their local application of the pneumatic pressure ejection under current clamp. the peptides tested and their effects were as follows: ser2-mip (inhibitory). carp (inhibitory), oxytocin (excitatory), spcb (excitatory), alpha-bcp (no effect). elh (no effect). apgw-amide (inhibitory) and fmrfamide (inhibitory). membrane conductance (g) of a neurone, d-rpean, measured under voltage clamp, was unexpe ... | 1993 | 8098562 |
| growth hormone and gonadotropin secretion in the common carp (cyprinus carpio l.): in vitro interactions of gonadotropin-releasing hormone, somatostatin, and the dopamine agonist apomorphine. | the effects of salmon gonadotropin-releasing hormone (sgnrh) and the superactive agonist [d-arg6, pro9net]-sgnrh (sgnrh-a) on growth hormone (gh) and gonadotropin (gth) release were examined using a perifusion system for pituitary fragments of the common carp (cyprinus carpio). perifusion of 2-min pulses of different concentrations of sgnrh or sgnrh-a stimulated a rapid and dose-dependent increase in gh release: ed50 values for sgnrh and sgnrh-a in stimulating gh release were 2.8 +/- 0.7 and 0.5 ... | 1993 | 8094060 |
| subunit structure of multiple hemoglobins in carp. | three hemoglobin components in carp designated ci, cii, and ciii, were isolated by deae-tokyo-pearl ion-exchange chromatography. constituent globin chains, alpha 1, alpha 2, beta 1, and beta 2, were analyzed by urea-triton acid polyacrylamide gel electrophoresis and isolated by high performance liquid chromatography with a reverse-phase column. tryptic peptide mapping indicated that the alpha-globin chains of the three hemoglobin components have slightly different structures. in addition, n-term ... | 1993 | 8071464 |
| effect of sublethal concentration of mercury and zinc on the energetics of a freshwater fish cyprinus carpio (linnaeus). | exposure of a freshwater fish cyprinus carpio to the sublethal concentration of mercury (0.1 mg l-1) and zinc (6.0 mg l-1) resulted in distinct changes in the energy metabolism of gill, liver and muscle at 1, 15 and 30 days. the changes were: (a) the rate of oxygen consumption and sdh activity decreased in the organs of mercury-exposed fish at all the three exposure periods in the order 1 > 15 < 30 days. whereas, an increase was observed in these parameters in the organs of zinc-exposed fish in ... | 1993 | 7871926 |
| peptidergic and aminergic centers in the helix cerebral ganglia: somatotopy and immunocytochemistry. | in helix cerebral ganglia the relationship between location of serotonin, dopamine, carp, fmrfamide, met-enkephalin, and substance p immunoreactive neurons and the different representation foci of the head areas was compared. the majority of immunoreactive neurons was located in representation foci, however, the extension of immunoreactive centers are larger than the representation foci. therefore, the real extension of a representation focus with the additional non-efferent neurons might be lar ... | 1993 | 7684175 |
| reprogramming of nucleolar gene expression during the acclimatization of the carp. | during seasonal acclimatization of eurythermal fish, the nucleolus of the hepatocyte undergoes ultrastructural reprogramming. in winter acclimatized carp, the nucleolar components are segregated, a condition that suggests a decreased transcription of rrna. the same nucleolar reorganization was observed when pituitary cells from winter- and summer-acclimatized carp were examined. in situ analyses of nucleolar rna revealed a marked lowering of rna content in the segregated nucleoli. accordingly, i ... | 1993 | 7519943 |
| optical probing of active membrane potential using lasers and microoptics. | we developed an optical probing system of active membrane potentials using lasers and microoptics. we measured the action potential of carp heart by multiple-wavelength and multiple-site optical probing system. taking advantage of the coherent laser beam, an optical microprobing method for micro-study on ion channel activity is proposed. | 1993 | 7505857 |
| the phylogenetic position of the zebrafish (danio rerio), a model system in developmental biology: an invitation to the comparative method. | the zebrafish, danio (brachydanio) rerio, has become one of the most widely studied model systems in developmental biology. we present a dna-based phylogeny of zebrafish and other species of the genus danio, and the genera rasbora, puntius and cyprinus. homologous regions of the large (16s) mitochondrial ribosomal rna gene were amplified by the polymerase chain reaction and directly sequenced. the phylogeny revealed: (i) the zebrafish, danio (brachydanio) rerio, is identical in its 16s sequence ... | 1993 | 8394584 |
| a pulsed photolysis procedure for determining oxygen equilibrium parameters of low-affinity noncooperative hemoglobins. | it is often very difficult to obtain precise values for oxygen equilibrium constants for low-affinity noncooperative hemoglobins owing to the ease with which they are oxidized and denatured with even the most gentle stirring or agitation. we have developed a procedure that eliminates this problem by maintaining the hemoglobin as hbco except during the brief time it is present as hb, and hbo2 forms following photolysis. oxygen is continuously removed by an enzyme system. the solution is photolyze ... | 1993 | 8367536 |
| in vitro phosphorylation in isolated horizontal cells of the fish retina: effects of the state of light adaptation. | horizontal cells, which are second-order neurons of the vertebrate retina, exhibit synaptic plasticity governed by light and dark adaptation. we have investigated the alterations in the protein phosphorylation patterns of isolated carp (cyprinus carpio) horizontal cells in relation to their state of light adaptation by using an in vitro phosphorylation assay and compared the resulting data with protein synthesis patterns of the whole retina. phosphoproteins and [35s]methionine-labelled proteins ... | 1993 | 8261133 |
| studies on the hypocalcemic actions of salmon calcitonin and ultimobranchial gland extracts in the freshwater teleost cyprinus carpio. | a single injection of synthetic salmon calcitonin (sct) or homologous ultimobranchial gland (ubg) extract caused a significant reduction in total and ultrafiltrable plasma ca2+ in a freshwater teleost, cyprinus carpio. a time-bound analysis on the effect of sct or ubg extract caused a short-duration hypocalcemia and the response was more pronounced in fish kept in low-ca2+ water. sexually immature fish showed a greater response than did mature fish. in all the studies the protein-bound ca2+ frac ... | 1993 | 8224753 |
| toxic effects of industrial effluents on hatchability and viability of cyprinus carpio eggs. | 1993 | 8387839 | |
| risk factors for kidney cancer in new south wales. iv. occupation. | in a population based case-control study of kidney cancer in new south wales, data from structured interviews with 489 cases of renal cell cancer (rcc), 147 cases of renal pelvic cancer (carp), and 523 controls from the electoral roles were obtained about employment in certain industries or occupations, and exposure to particular chemicals chosen because of suspected associations with kidney cancer. a low level of education increased the risk for carp but not rcc. after adjustment for known risk ... | 1993 | 8494775 |
| variable sensitivity of cyprinus carpio eggs, larvae, and fry to pesticides. | 1993 | 8467147 | |
| mimosine, a naturally occurring drug interfering primarily with the cell nucleus. | the non-proteinogen aminoacid mimosine interfered seriously with cultured carp hepatocytes when added to the culture medium for 6 h in a concentration of 0.1 m. cell transformation started with condensation of the chromatin into an unusual network of fibrils and segregation of the reticulate nucleolus into its fibrillar and granular components. then chromatin condensation spread homogeneously across the entire nucleus. at the end of the condensation period the nuclear envelope extended towards t ... | 1993 | 7686812 |
| zinc (zn2+) and fish immune response effect on carp il2-like production and activity. | supernatants obtained from pha-activated pronephric carp lymphocytes were able to provoke the growth of carp blasts but not from fresh lymphocytes without mitogens. these supernatants contain certainly soluble growth factor(s) such as il2. the addition of zn2+ (1 mm) to lymphocyte cultures could not enhance supernatants' ability to promote blast proliferation. when zn2+ and pha (50 micrograms/ml) were added, respectively, supernatants obtained could enhance significant tritiated thymidine incorp ... | 1993 | 7682507 |
| acute and subacute toxicity of atrazine to carp (cyprinus carpio l.). | investigations of acute and subacute atrazine toxicity in carp (cyprinus carpio l.) were carried out. acute toxicity was investigated in a semi-static test during a 96-hr exposition. the estimated lc-50 value was 18.8 mg/l. subacute toxicity was investigated by exposing fish (carp) to different atrazine concentrations (1.5, 3.0, and 6.0 mg/l) for 14 days. biochemical and histopathological changes in certain organs and tissues were investigated. the results show that atrazine leads to changes of ... | 1993 | 7682501 |
| steroidogenesis by ovaries and testes of the european catfish, the wels (silurus glanis), in vitro. | testosterone, 3α,17-dihydroxy-5β-pregnen-20-one, 17,20β-dihydroxy-4-pregnen-3-one (17,20βp) and 5β-pregnane-3α,17,20β-triol were identified as the major metabolites of [(3)h] 17-hydroxyprogesterone in ovarian incubations of the european catfish silurus glanis. 17,20βp and the reduced triol were present only in ovaries from fish primed with carp hypophysial homogenate (chh) while testosterone yields were significantly higher in controls than in treated fish. 11-ketotestosterone, 11β-hydroxytestos ... | 1993 | 24214377 |
| efficacy of fumagillin against thelohanellus kitauei infection of israel carp, cyprinus carpio nudus. | the potential of fumagillin dicyclohexylamine salt to treat and prevent intestinal giant-cystic disease in israel carp, cyprinus carpio nudus, was monitored in field experimental studies. in experiment 1 (therapeutic), most fish were already naturally infected with more advanced stage of thelohanellus kitauei. fumagillin was administered to fish (mean body weight of 830 g) for a period of one month at a dose of 10.62 mg in the first group and 5.3 mg in the second group per fish per day. in exper ... | 1993 | 8512900 |
| pharmacokinetics of sulphadiazine and trimethoprim in carp (cyprinus carpio l.) acclimated at two different temperatures. | 1993 | 8478994 | |
| involvement of fibronectin during epiboly and gastrulation in embryos of the common carp, cyprinus carpio. | the present report firstly describes a pilot study in which, during early development of embryos of the common carp, cyprinus carpio, the cellular adhesion to fibronectin (fn) was blocked by administration of grgds peptide (which binds to the fn-receptor). as this treatment resulted in developmental aberrations, suggesting a functional role for fn, the major part of the work was focussed on the distribution of reactivity of anti-fn antibodies during epiboly and gastrulation. grgds treatment had ... | 1993 | 28305992 |
| spinule-type neurite outgrowth from horizontal cells during light adaptation in the carp retina: an actin-dependent process. | dendrites of horizontal cells in the carp retina which invaginate the cone pedicles form numerous spinules during light adaptation. we have analyzed the contribution of cytoskeletal elements to this process. isolated horizontal cells and frozen sections were screened with phalloidin for the existence of f-actin. f-actin was present in all types of horizontal cells and particularly enriched in the distal parts of the dendrites. electron microscopical analysis demonstrated that interruption of the ... | 1993 | 8445409 |
| acute toxicity of selected organophosphorus pesticides to cyprinus carpio and barilius vagra. | the acute toxicity of two organophosphorus (op) pesticides, diazinon and malathion to cyprinus carpio and barilius vagra was determined. mortalities and lc50-96 hr values for b. vagra and c. carpio were variable. both juvenile common carp and b. vagra were extremely sensitive to diazinon. adult b. vagra were more sensitive to malathion than were juvenile common carp. | 1993 | 8426062 |
| hypo-osmotic shock induces an osmolality-dependent permeabilization and structural changes in the membrane of carp sperm. | we carried out spectrofluorimetric and flow cytometric measurements to investigate the effect of hypo-osmotic shock on cell membranes of common carp sperm. the time course of the permeability of the sperm cell membrane, as monitored by dna-related propidium iodide (pi) fluorescence, was followed for 30 min after dilution of semen in hypo-osmotic environments of different ionic strengths. spectrofluorimetric measurements indicated a continuous increase in the total pi emission intensity of a sper ... | 1993 | 8419464 |
| gene transfer by microinjection in the zebra fish brachydanio rerio. | although transgenesis studies of fish started relatively later compared to those of mammals, they have not been completely neglected, and in recent years, many publications have appeared in this field. reviews of transgenesis in fish are given by maclean et al., hew, maclean and penman, and ozato et al. much attention had been focused on commercially important food species, especially in the attempt to insert beneficial genes, such as the antifreeze and growth-hormone genes, into salmonids, such ... | 1993 | 21390647 |
| [digestion, growth and body composition of carp (cyprinus carpio l.) after feeding different starch varieties]. | maize starch (treatment i), pregelatinized maize starch (ii), wheat starch (iii), pregelatinized wheat starch (iv), potato starch (v) or manioc meal (vi) were mixed in portions of 41% (i-v) or 46.7% (vi) to a basal diet. each experimental diet was fed to 33 carp (cyprinus carpio l.) (three aquaria with 11 carp each) in a daily amount of 1.8-2.0% of live weight. the experiment lasted 118 days and the carp had a mean live weight of 146 g at the beginning. the diets i-iv had a similar, high energy ... | 1993 | 8517771 |
| characterization of catch-relaxing peptide (carp) immunoreactive neurons in the helix nervous system. | both small and large diameter of carp immunoreactive neurons could be observed in the different ganglia of cns of helix but not in the foot musculature. the immunoreactivity is the strongest in the varicose segments of immunoreactive fibers. the present findings suggest a transmitter or modulatory role of carp in both central and peripheral regulatory processes. | 1993 | 8493855 |
| new beta chain-encoding mhc class ii genes in the carp. | 1993 | 8482577 | |
| filament-forming domain of carp dorsal myosin rod. | substructure of the myosin rod and its correlation to the filament formation were investigated by using fish myosin rod. it was found that fish rod contains a unique chymotrypsin susceptible site, 40 kda from the cooh-terminus or 20 kda downstream from the subfragment-2/light meromyosin junction (s-2/lmm junction). cleavage at this new site produced subfragment-2 possessing 95 kda subunit (95k s-2) and light meromyosin possessing 40 kda subunit (40k lmm). the latter is the shortest unit ever rep ... | 1993 | 8454573 |
| localization of extratectally evoked visual response in the corpus and valvula cerebelli in carp, and cerebellar contribution to 'dorsal light reaction' behavior. | in carp with the optic tectum removed bilaterally, the optic nerve stimulation evoked a surface positive response on the cerebellum, which originated in the granular layer. in the corpus cerebelli, the response was composed of a single component, well localized at the rostromedial part, supporting previous electrophysiological and morphological findings. in the valvula cerebelli, on the other hand, the response consisted of two wave components of oligo-synaptic and poly-synaptic transmission. th ... | 1993 | 8155291 |
| effect of lethal and sublethal concentrations of cadmium on energetics in the gills of fry and fingerlings of cyprinus carpio. | 1993 | 8257822 | |
| [structure analysis of the mitochondrial trna(cys) gene and the original region of light-strand replication from carp]. | in this study, we have determined the nucleotide sequences of the cysteine trna gene and the original region of light-strand replication from carp (cyprinus carpio l.) mitochondrion, which were further represented respectively in cloverleaf secondary structure and stem loop structure. by comparing the nucleotide sequences of 5 vertebrate trna(cys) genes, it is found that carp mitochondrial trna(cys) gene have many unusual structural features differing from those of cytoplasmic trna(cys) gene. th ... | 1993 | 8023638 |
| oral administration of insulin in winter-acclimatized carp (cyprinus carpio) induces hepatic ultrastructural changes. | 1. the intestinal absorption of insulin in carps was assessed examining the transepithelial passage of ingested gold-labeled hormone by electron microscopy. insulin transfer occurred mainly through the intercellular spaces between the enterocytes. 2. when reaching the lamina propria, the gold-labeled hormone gathered predominantly around the granules of the granular cells, and therefore can enter the circulatory system via the blood capillaries which are found in close contact with these cells. ... | 1993 | 7906626 |
| 'broken backs' in koi carp (cyprinus carpio) following lightning strike. | 1993 | 8296436 | |
| dendrites of rod dominant on-bipolar cells are coupled by gap junctions in carp retina. | gap junctions are supposed to be the anatomical substrate for electrical coupling between neurons. in fish retina, bipolar cells are electrically coupled and their receptive field diameters are always larger than dendritic field size, however, gap junctions have not been described between dendrites of bipolar cells. in this paper, using immunostaining for protein kinase c, we show that every rod dominant on-bipolar cell is connected with its neighboring dendrites by gap junctions forming a plexu ... | 1993 | 8121632 |
| feeding of an iberian stream cyprinid assemblage: seasonality of resource use in a highly variable environment. | food resource use by seven cyprinids from an iberian stream was analysed over 9 months. differences in food resource use were found both between species and within species between seasons. plant material was a more important food for carp, nase, goldfish and barbel than for gudgeon, roach and chub, irrespective of the season. chironomid larvae were the staple animal food for the former five species throughout the year. roach and chub, especially the latter, displayed large seasonal variations in ... | 1993 | 28313422 |
| the effect of 75% glycerol on the oxygen binding properties of carp hemoglobin. | at ph 6 in the presence of inositol hexaphosphate, ihp, conditions where ligand-saturated carp hemoglobin is already in the low affinity t state, the addition of glycerol has little effect on affinity and ligand binding remains noncooperative. at all other ph values examined, with and without ihp, the effect of glycerol is to lower oxygen affinity possibly by shifting the equilibrium between the t state and the high affinity r state in the direction of the t state. although glycerol does not app ... | 1993 | 8216252 |
| neuropeptide y in the carp torus semicircularis: an immunocytochemical study. | the presence of neuropeptide y-like immunoreactivity in the torus semicircularis of the teleost fish cyprinus carpio was studied using an indirect immunoperoxidase technique. in general, both the nuclei lateralis and centralis of the torus semicircularis showed a high density of immunoreactive fibers from rostral to caudal levels. in particular, in the nucleus lateralis a low density of immunoreactive fibers was only found in the subependymal layer, whereas this was high in the small cell, fibri ... | 1993 | 8250673 |
| the marker for nitric oxide synthase, nadph-diaphorase, co-localizes with gaba in horizontal cells and cells of the inner retina in the carp retina. | nadph-diaphorase histochemistry selectively stained discrete populations of neurons contributing to the inner plexiform layer of the carp retina. in addition h1 horizontal cells contributing to the outer plexiform layer were labeled. in these cells as in a subpopulation of the labeled cells in the inner retina, nadph-activity co-localized with gaba immunoreactivity. nadph-activity is a direct marker for no synthesis and it is concluded that no might be an important mediator of light-dependent ad ... | 1993 | 7694204 |
| cloning and characteristics of fish glial fibrillary acidic protein: implications for optic nerve regeneration. | mammalian central nervous system neurons do not regenerate after axonal injury, unlike their counterparts in fish and amphibians. after axonal injury, glial cells in mammals do not support regrowth of axons, while in fish they support the regeneration process. controversy exists as to whether or not the intact fish optic nerve expresses glial fibrillary acidic protein, a well-known marker for mature astrocytes, and thus whether its astrocytes differ in this respect from those of the brain and sp ... | 1993 | 8376626 |
| hormonal replacement therapy in fish:human growth hormone gene function in hypophysectomized carp. | transgenic common carp,cyprinus carpio, produced by the microinjection of fertilized eggs with a linearized chimeric plasmid pmthgh, a human growth hormone (hgh) gene with a mouse metallothionein-1 (mt) gene promoter in pbr322, were used to produce f1 and f2 transgenics. following hypophysectomy of the transgenic f2 common carp, non-transgenic common carp and non-transgenic crucian carp, growth was monitored for up to 110 days. in addition, recombinant hgh was injected subcutaenously into a grou ... | 1993 | 24202695 |
| neuropeptides and monoamines in the carp (cyprinus carpio) pretectum: an immunocytochemical study. | the distribution of neurotensin, neurokinin a, dynorphin a, galanin, somatostatin-28 (1-12), neuropeptide y, vasoactive intestinal polypeptide, gastrin-releasing peptide, gamma-melanocyte stimulating hormone, alpha-neo-endorphin, angiotensin h, cholecystokinin-8, serotonin and tyrosine hydroxylase has been studied in the pretectal nuclei of the cyprinus carpio: nuclei pretectalis superficialis parvicellularis and magnocellularis, pretectalis centralis, pretectalis, and pretectalis periventricula ... | 1993 | 18621246 |
| cyclic nucleotide-activated channels in carp olfactory receptor cells. | when applied from the cytoplasmic side, cyclic 3',5'-adenosine and guanosine monophosphates reversibly increased the ion permeability of inside-out patches of carp olfactory neuron plasma membrane. the camp (cgmp)-induced permeability via camp (cgmp) concentration was fitted by hill's equation with the exponents of 1.07 +/- 0.15 (1.12 +/- 0.05) and ec50 = 1.3 +/- 0.6 microm (0.9 +/- 0.3 microm). substitution of nacl in the bathing solution by chlorides of other alkali metals resulted in a slight ... | 1993 | 8334139 |
| binding and bioactivity of insulin in primary cultures of carp (cyprinus carpio) hepatocytes. | isolated carp hepatocytes cultured in serum-free, chemically defined medium were used to investigate within the same cell preparation characteristics of the binding of insulin as well as effects of insulin on cellular metabolism. the binding of human [(125)i]-insulin to carp hepatocytes was studied in kinetic, saturation and displacement experiments. a dependency of insulin binding on the collagenase used for cell isolation was demonstrated. insulin binding decreased during the first 12h of cult ... | 1993 | 24202501 |
| all-fish gene constructs for growth hormone gene transfer in fish. | in order to develop all-fish expression vectors for microinjection into fertilized fish eggs, we have prepared the following constructs: rainbow trout metallothionein a/b and the gilthead seabream growth hormone cdna (ptmta-gbsghcdna, ptmtb-gsbghcdna), carp β-actin gilthead seabream gh cdna (pcaβ-gsbghcdna). the inducible metallothionein promoters a and b were cloned from rainbow trout, and the constitutive promoter β-actin was isolated from carp.the metallothionein promoters were cloned by usin ... | 1993 | 24202494 |
| insulin and igf-i receptors and tyrosine kinase activity in carp ovaries: changes with reproductive cycle. | insulin and insulin-like growth factor i (igf-i) receptors from carp ovaries were semipurified with wheat germ agglutinin at different moments of the reproductive cycle and their binding characteristics and tyrosine kinase activity were studied. specific receptors for insulin and igf-i were found. igf-i receptors presented higher binding (23.8 ± 1.5%), number of receptors (965 ± 20fm/mg) and affinity (kd 0.24 ± 0.03nm) than those shown for insulin receptors (4.1 ± 1%, 530 ± 85fm/mg and 0.85 ± 0. ... | 1993 | 24202482 |
| the regulatory effects of thyrotropin-releasing hormone on growth hormone secretion from the pituitary of common carp in vitro. | the effects of thyrotropin-releasing hormone (trh) on growth hormone (gh) and gonadotropin (gth) release, and the influences of somatostatin (srif), the dopamine agonist apomorphine (apo) and extracellular calcium on basal and trh-induced gh release were examined using an in vitro perifusion system for pituitary fragments of common carp (cyprinus carpio). five minute pulses of different dosages of trh stimulated a rapid and dose-dependent increase in gh release from the perifused pituitary fragm ... | 1993 | 24202462 |
| rapid and simultaneous measurement of anodic and cathodic haemoglobins and atp and gtp concentrations in minute quantities of fish blood | oxygen transport to vertebrate tissues is dependent upon (a) the intrinsic o2-binding properties of haemoglobin (hb) and (b) modulation by allosteric effectors, such as nucleoside triphosphates (ntp), that depress hb o2-affinity in fish red cells. while some species, such as flatfish, primarily use adenosine triphosphate (atp) to modulate o2-binding to hb, others, such as eel and carp, also use guanosine triphosphate (gtp) (kono and hashimoto, 1977; leray, 1982; weber and jensen, 1988). unlike m ... | 1994 | 9317794 |
| cloning of a growth hormone from a primitive bony fish and its phylogenetic relationships. | growth hormone (gh), prolactin, and their relatives constitute a multigene family which is considered to have evolved from a common ancestor. the structural and functional domains of gh appear to be highly conserved among vertebrates. in order to investigate the phylogenetic relationships among ghs in the actinopterygii and sarcopterygii, we have cloned and sequenced gh from the pituitary of the primitive bony fish, amia calva. bony fishes (teleosts) and amia (halecomorphi) are purported sister- ... | 1994 | 7926657 |
| double-staining of horizontal and amacrine cells by intracellular injection with lucifer yellow and biocytin in carp retina. | horizontal and amacrine cells in the isolated carp retina were impaled with micropipette electrode, identified by their characteristic light responses, and injected iontophoretically with markers for morphological study. both lucifer yellow ch and biocytin were injected simultaneously. lucifer yellow was seen by its own fluorescence while biocytin was visualized by binding with texas red-linked or horseradish peroxidase-conjugated avidin. for cone-connected horizontal cells, biocytin-coupled cel ... | 1994 | 7514770 |