Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| purification and sequence determination of an rna ligase from wheat embryos. | wheat embryos have an rna ligase activity that catalyses ligation of rna molecules between the 5'-hydroxyl and 2',3'-cyclic phosphate termini. in contrast to yeast trna ligase, which ligates trna half molecules specifically, the wheat enzyme acts on various rna substrates. it has been suggested that rna ligase plays some unknown roles in vivo other than the maturation of trna. here, we report purification of the enzyme and determination of the protease-digested peptide sequences. the sequences s ... | 2005 | 17150762 |
| high-level production of yeast (schwanniomyces occidentalis) phytase in transgenic rice plants by a combination of signal sequence and codon modification of the phytase gene. | this study was designed to produce yeast (schwanniomyces occidentalis) phytase in rice with a view to future applications in the animal feed industry. to achieve high-level production, chimeric genes with the secretory signal sequence of the rice chitinase-3 gene were constructed using either the original full-length or n-truncated yeast phytase gene, or a modified gene whose codon usage was changed to be more similar to that of rice, and then introduced into rice (oryza sativa l.). when the ori ... | 2005 | 17168898 |
| expression of escherichia coli branching enzyme in caryopses of transgenic rice results in amylopectin with an increased degree of branching. | physiochemical properties of starch are dependent on several factors including the relative abundance of amylose and amylopectin, and the degree of branching of amylopectin. utilizing agrobacterium-mediated transformation, a construct containing the coding region of branching enzyme of escherichia coli, under transcriptional control of the rice (oryza sativa l.) starch-branching enzyme promoter was introduced into rice cv. nakdong. to enhance glgb expression, the first intron of rice starch-bran ... | 2005 | 15517356 |
| spatial control of transgene expression in rice (oryza sativa l.) using the gal4 enhancer trapping system. | we used enhancer trapping with the gal4 transcriptional activator from yeast to obtain spatial control of transgene expression in all organs of the model monocotyledonous species rice (oryza sativa l. cv. nipponbare). our t-dna enhancer trapping cassette consisted of two principle components: (1) the minimal promoter-equipped gal4 gene placed adjacent to the right border, and (2) the green fluorescent protein gene (gfp) fused to the upstream activation sequence element (uas) to which gal4 binds ... | 2005 | 15703064 |
| characterization of a novel plant growth-promoting bacteria strain delftia tsuruhatensis hr4 both as a diazotroph and a potential biocontrol agent against various plant pathogens. | a novel, plant growth-promoting bacterium delftia tsuruhatensis, strain hr4, was isolated from the rhizoplane of rice (oryza sativa l., cv. yueguang) in north china. in vitro antagonistic assay showed this strain could suppress the growth of various plant pathogens effectively, especially the three main rice pathogens (xanthomonas oryzae pv. oryzae, rhizoctonia solani and pyricularia oryzae cavara). treated with strain hr4 culture, rice blast, rice bacterial blight and rice sheath blight for cv. ... | 2005 | 15709367 |
| gc-compositional strand bias around transcription start sites in plants and fungi. | a gc-compositional strand bias or gc-skew (=(c-g)/(c+g)), where c and g denote the numbers of cytosine and guanine residues, was recently reported near the transcription start sites (tss) of arabidopsis genes. however, it is unclear whether other eukaryotic species have equally prominent gc-skews, and the biological meaning of this trait remains unknown. | 2005 | 15733327 |
| rice (oryza sativa l.) containing the bar gene is compositionally equivalent to the nontransgenic counterpart. | this publication presents an approach to assessing compositional equivalence between grain derived from glufosinate-tolerant rice grain, genetic event llrice62, and its nontransgenic counterpart. rice was grown in the same manner as is common for commercial production, using either conventional weed control practices or glufosinate-ammonium herbicide. a two-season multisite trial design provided a robust data set to evaluate environmental effects between the sites. statistical comparisons to tes ... | 2005 | 15740024 |
| a mitogen-activated protein kinase cascade regulating infection-related morphogenesis in magnaporthe grisea. | many fungal pathogens invade plants by means of specialized infection structures called appressoria. in the rice (oryza sativa) blast fungus magnaporthe grisea, the pathogenicity mitogen-activated protein (map) kinase1 (pmk1) kinase is essential for appressorium formation and invasive growth. in this study, we functionally characterized the mst7 and mst11 genes of m. grisea that are homologous with the yeast map kinase kinase ste7 and map kinase kinase kinase ste11. similar to the pmk1 mutant, t ... | 2005 | 15749760 |
| molecular cloning and expression analysis of the cell-wall invertase gene family in rice (oryza sativa l.). | cell-wall invertase (cin) catalyzes the hydrolysis of sucrose into glucose and fructose for the supply of carbohydrates to sink organs via an apoplastic pathway. to study the cin genes in rice (oryza sativa l.), we isolated cdna clones showing amino acid similarity to the plant cell wall invertase proteins from a search of rice sequence databases. profile analyses revealed that the cloned genes are expressed in unique patterns in various organs. for example, transcripts of oscin1, oscin2, oscin4 ... | 2005 | 15759120 |
| spliced leader rna-mediated trans-splicing in phylum rotifera. | in kinetoplastids, euglena, and four metazoan phyla, trans-splicing has been described as a mechanism for the generation of mature messenger rnas (mrnas): 5'-ends of precursor mrnas are replaced by a short spliced leader (sl) exon from a small sl rna. although the full phylogenetic range is unknown, trans-splicing has not been found in vertebrates, insects, plants, or yeast. in animal groups where it does occur, i.e., nematodes, cnidarians, platyhelminths, and primitive chordates, sl rnas do not ... | 2005 | 15788744 |
| the genome sequence of xanthomonas oryzae pathovar oryzae kacc10331, the bacterial blight pathogen of rice. | the nucleotide sequence was determined for the genome of xanthomonas oryzae pathovar oryzae (xoo) kacc10331, a bacterium that causes bacterial blight in rice (oryza sativa l.). the genome is comprised of a single, 4 941 439 bp, circular chromosome that is g + c rich (63.7%). the genome includes 4637 open reading frames (orfs) of which 3340 (72.0%) could be assigned putative function. orthologs for 80% of the predicted xoo genes were found in the previously reported x.axonopodis pv. citri (xac) a ... | 2005 | 15673718 |
| identification of candidate markers associated with agronomic traits in rice using discriminant analysis. | plant genetic mapping strategies routinely utilize marker genotype frequencies obtained from progeny of controlled crosses to declare presence of a quantitative trait locus (qtl) on previously constructed linkage maps. we have evaluated the potential of discriminant analysis (da), a multivariate statistical procedure, to detect candidate markers associated with agronomic traits among inbred lines of rice (oryza sativa l.). a total of 218 lines originating from the us and asia were planted in fie ... | 2005 | 15678327 |
| phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase-related proteins. | protein disulfide isomerases (pdis) are molecular chaperones that contain thioredoxin (trx) domains and aid in the formation of proper disulfide bonds during protein folding. to identify plant pdi-like (pdil) proteins, a genome-wide search of arabidopsis (arabidopsis thaliana) was carried out to produce a comprehensive list of 104 genes encoding proteins with trx domains. phylogenetic analysis was conducted for these sequences using bayesian and maximum-likelihood methods. the resulting phylogen ... | 2005 | 15684019 |
| the genomes of oryza sativa: a history of duplications. | we report improved whole-genome shotgun sequences for the genomes of indica and japonica rice, both with multimegabase contiguity, or almost 1,000-fold improvement over the drafts of 2002. tested against a nonredundant collection of 19,079 full-length cdnas, 97.7% of the genes are aligned, without fragmentation, to the mapped super-scaffolds of one or the other genome. we introduce a gene identification procedure for plants that does not rely on similarity to known genes to remove erroneous pred ... | 2005 | 15685292 |
| purification and characterization of two ascorbate peroxidases of rice (oryza sativa l.) expressed in escherichia coli. | to clarify the diversity and function of isozymes of ascorbate peroxidase (apx) in plants, a method of producing large quantities of these proteins is needed. here, we describe an escherichia coli expression system for the rapid and economic expression of two rice apx genes, apxa and apxb (genebank accession nos. d45423 and ab053297, respectively). the two genes were cloned into the pgex-6p-3 vector to allow expression of apx as a glutathione-s-transferase (gst) fusion protein. the gst-apxa and ... | 2005 | 15685422 |
| biodiversity of rice (oryza sativa l.) and sugarcane (saccharum officinarum l.) rhizosphere pseudomonads. | fluorescent pseudomonads were isolated from the rhizosphere of rice and sugarcane and examined for their biodiversity. all fifty strains of the fluorescent pseudomonads produced indole acetic acid. among these pseudomonads, halves of sugarcane rhizosphere isolates and one isolate from the rice rhizosphere exhibited phosphate solubilization activity. on the contrary, majority of the rice rhizosphere pseudomonads, and one isolate from sugarcane rhizosphere exhibited antifungal activity. these fluo ... | 2005 | 15691070 |
| mir spectroscopic analysis on sugar metabolic and ethanol productive kinetics of suspension tby-2 and rice cells pre-cultured in various media. | the influence of sugars in pre-cultivation media suspended plant cells on the kinetics of the sugar uptake and the ethanol production was studied by mid-infrared spectroscopy using a fourier transform infrared spectrometer (ft-ir) equipped with an attenuate total reflection accessory (atr). we performed the plant cell cultivation with nicotiana tabacum cv. bright yellow no.2 (tby-2) cells and oryza sativa l., japonica, cv. nipponbare (rice) cells, respectively, in pre-culture and culture media, ... | 2005 | 15654611 |
| grain growth and endosperm cell size under high night temperatures in rice (oryza sativa l.). | high night temperatures are more harmful to grain weight in rice than high day temperatures. grain growth rate and growth duration were investigated to determine which was the cause of the decrease in final grain weight under high night temperatures. endosperm cell number and cell sizes were also examined to determine which might cause the decrease in final grain weight. | 2005 | 15655104 |
| the oslti6 genes encoding low-molecular-weight membrane proteins are differentially expressed in rice cultivars with contrasting sensitivity to low temperature. | rice (oryza sativa l.) is sensitive to chilling particularly at early stages of seedling establishment. two closely related genes (oslti6a, oslti6b), which are induced by low temperature during seedling emergence were isolated from a cold tolerant temperate japonica rice cultivar. these genes are closely related to the arabidopsis rare cold-inducible (rci2) and barley low-temperature-inducible (blt101) genes. based on direct biochemical and indirect physiological evidence and similarity with a c ... | 2005 | 15656983 |
| functional isolation of novel nuclear proteins showing a variety of subnuclear localizations. | nuclear proteins play key roles in the fundamental regulation of genome instability, the phases of organ development, and physiological responsiveness through gene expression. although nuclear proteins have been shown to account for approximately one-fourth of total proteins in yeast, no efficient method to identify novel nuclear proteins has been applied to plants. in this study, a trial to isolate nuclear proteins in rice was attempted, and several novel nuclear proteins showing a variety of s ... | 2005 | 15659629 |
| inparanoid: a comprehensive database of eukaryotic orthologs. | the inparanoid eukaryotic ortholog database (http://inparanoid.cgb.ki.se/) is a collection of pairwise ortholog groups between 17 whole genomes; anopheles gambiae, caenorhabditis briggsae, caenorhabditis elegans, drosophila melanogaster, danio rerio, takifugu rubripes, gallus gallus, homo sapiens, mus musculus, pan troglodytes, rattus norvegicus, oryza sativa, plasmodium falciparum, arabidopsis thaliana, escherichia coli, saccharomyces cerevisiae and schizosaccharomyces pombe. complete proteomes ... | 2005 | 15608241 |
| the rice (oryza sativa) blast lesion mimic mutant, blm, may confer resistance to blast pathogens by triggering multiple defense-associated signaling pathways. | here we characterized a rice (oryza sativa l.) blast lesion mimic (blm) mutant, identified previously in an n-methyl-n-nitrosourea-mutagenized population of the cultivar hwacheong (wild type). the rice blm displayed spontaneous necrotic lesion formation on the leaves during development under long-day condition and temperature shift from 28 to 24 degrees c in the absence of obvious stress/disease, and provided us with a highly reproducible and convenient experimental system in the growth chamber ... | 2005 | 15907692 |
| maximal sequence length of exact match between members from a gene family during early evolution. | mutation (substitution, deletion, insertion, etc.) in nucleotide acid causes the maximal sequence lengths of exact match (male) between paralogous members from a duplicate event to become shorter during evolution. in this work, male changes between members of 26 gene families from four representative species (arabidopsis thaliana, oryza sativa, mus musculus and homo sapiens) were investigated. comparative study of paralogous' male and amino acid substitution rate (d(a)<0.5) indicated that a clos ... | 2005 | 15909329 |
| oslsd1, a rice zinc finger protein, regulates programmed cell death and callus differentiation. | the arabidopsis lsd1 and lol1 proteins both contain three conserved zinc finger domains and have antagonistic effects on plant programmed cell death (pcd). in this study, a rice (oryza sativa) functional homolog of lsd1, designated oslsd1, was identified. the expression of oslsd1 was light-induced or dark-suppressed. overexpression of oslsd1 driven by the cauliflower mosaic virus 35s promoter accelerated callus differentiation in transformed rice tissues and increased chlorophyll b content in tr ... | 2005 | 15915636 |
| mapping of qtls conferring extremely early heading in rice (oryza sativa l.). | two genes related to extremely early heading were identified in populations derived from crosses between hoshinoyume, a variety adapted to the northernmost limit of rice cultivation (hokkaido), and nipponbare, a variety adapted to the temperate region of japan. the segregations for heading date clearly revealed that a two-gene model determined the extremely early heading in the f(2) and bc(1)f(1) populations under natural field conditions in hokkaido. using molecular markers corresponding to ten ... | 2005 | 15940510 |
| molecular mapping of the fertility restorer gene for ms-cw-type cytoplasmic male sterility of rice. | cytoplasmic male sterility (cms) of rice (oryza sativa l.) was first reported using the cytoplasm of a chinese wild rice, oryza rufipogon griff. strain w1. however, it was not possible to characterize this ms-cw-type cms in more detail until a restorer line had been developed due to the lack of restorer genes among cultivars thus far tested. the breeding of a restorer line (w1-r) was eventually achieved by transferring the restorer gene(s) of w1 to a cultivar. we report here the characterization ... | 2005 | 15947907 |
| phylogenetic relationships among a-genome species of the genus oryza revealed by intron sequences of four nuclear genes. | the a-genome group in oryza consists of eight diploid species and is distributed world-wide. here we reconstructed the phylogeny among the a-genome species based on sequences of nuclear genes and mite (miniature inverted-repeat transposable elements) insertions. thirty-seven accessions representing two cultivated and six wild species from the a-genome group were sampled. introns of four nuclear single-copy genes on different chromosomes were sequenced and analysed by both maximum parsimony (mp) ... | 2005 | 15948847 |
| identification and mapping of yield and yield related qtls from an indian accession of oryza rufipogon. | cultivated rice (oryza sativa l.) is endowed with a rich genetic variability. in spite of such a great diversity, the modern rice cultivars have narrow genetic base for most of the agronomically important traits. to sustain the demand of an ever increasing population, new avenues have to be explored to increase the yield of rice. wild progenitor species present potential donor sources for complex traits such as yield and would help to realize the dream of sustained food security. | 2005 | 15949048 |
| phylogenomic analysis of the receptor-like proteins of rice and arabidopsis. | the tomato (lycopersicon esculentum) cf-9 resistance gene encodes the first characterized member of the plant receptor-like protein (rlp) family. other rlps such as clavata2 and too many mouths are known to regulate development. the domain structure of rlps consists of extracellular leucine-rich repeats, a transmembrane helix, and a short cytoplasmic region. here, we identify 90 rlps in rice (oryza sativa) and compare them with functionally characterized rlps from different plant species and wit ... | 2005 | 15955925 |
| pathogen-induced production of the antifungal afp protein from aspergillus giganteus confers resistance to the blast fungus magnaporthe grisea in transgenic rice. | rice blast, caused by magnaporthe grisea, is the most important fungal disease of cultivated rice worldwide. we have developed a strategy for creating disease resistance to m. grisea whereby pathogen-induced expression of the afp (antifungal protein) gene from aspergillus giganteus occurs in transgenic rice plants. here, we evaluated the activity of the promoters from three maize pathogenesis-related (pr) genes, zmpr4, mpi, and prms, in transgenic rice. chimeric gene fusions were prepared betwee ... | 2005 | 16167766 |
| two new sine elements, p-sine2 and p-sine3, from rice. | p-sine1 was the first plant sine element identified in the waxy gene in oryza sativa, and since then a large number of p-sine1-family members have been identified from rice species with the aa or non-aa genome. in this paper, we report two new rice sine elements, designated p-sine2 and p-sine3, which form distinct families from that of p-sine1. each of the two new elements is significantly homologous to p-sine1 in their 5'-end regions with that of the polymerase iii promoter (a box and b box), b ... | 2005 | 16172529 |
| biochemical character of the purified osraa1, a novel rice protein with gtp-binding activity, and its expression pattern in oryza sativa. | osraa1, as previously reported, is a novel conserved protein in plants and plays an important role in rice root development. overexpression of osraa1 results in reduced growth of primary roots and an increased number of adventitious roots. the biochemical functions and expression patterns of osraa1, however, remain poorly understood. to obtain purified osraa1 for biochemical analysis, the coding region was amplified by rt-pcr and expressed as a fusion protein with glutathione s-transferase in es ... | 2005 | 16173467 |
| production and characterization of a complete set of individual chromosome additions from oryza officinalis to oryza sativa using rflp and gish analyses. | monosomic alien addition lines (maals) are valuable materials for comparative analyses of two distinct genomes, for elucidating introgression mechanisms, and for dissecting genes controlling complex traits. in the study reported here, maals of rice containing the complete genome of oryza sativa and individual chromosomes of oryza officinalis were produced. interspecific hybridizations were made between o. sativa l. ssp. japonica (cv, hejiang 19, 2 n = 24, aa) and o. officinalis (acc. hy018, 2 n ... | 2005 | 16177899 |
| isolation of a calmodulin-binding transcription factor from rice (oryza sativa l.). | calmodulin (cam) regulates diverse cellular functions by modulating the activities of a variety of enzymes and proteins. however, direct modulation of transcription factors by cam has been poorly understood. in this study, we isolated a putative transcription factor by screening a rice cdna expression library by using cam:horse-radish peroxidase as a probe. this factor, which we have designated oscbt (oryza sativa cam-binding transcription factor), has structural features similar to arabidopsis ... | 2005 | 16192280 |
| high-resolution mapping of the barley leaf rust resistance gene rph5 using barley expressed sequence tags (ests) and synteny with rice. | the rapidly growing expressed sequence tag (est) resources of species representing the poacea family and availability of comprehensive sequence information for the rice (oryza sativa) genome create an excellent opportunity for comparative genome analysis. extensive synteny between rice chromosome 1 and barley (hordeum vulgare l.) chromosome 3 has proven extremely useful for saturation mapping of chromosomal regions containing target genes of large-genome barley with conserved orthologous genes f ... | 2005 | 16195886 |
| cloning and characterization of a rice cdna encoding glutamate decarboxylase. | in this study, we have isolated a rice (oryza sativa l.) glutamate decarboxylase (ricgad) clone from a root cdna library, using a partial arabidopsis thaliana gad gene as a probe. the rice root cdna library was constructed with mrna, which had been derived from the roots of rice seedlings subjected to phosphorus deprivation. nucleotide sequence analysis indicated that the ricgad clone was 1,712 bp long, and harbors a complete open reading frame of 505 amino acids. the 505 amino acid sequence ded ... | 2005 | 16202241 |
| isolation and characterization of a cdna encoding two novel heat-shock factor oshsf6 and oshsf12 in oryza sativa l. | as a crucial transcription factor family, heat-shock factors were mainly analyzed and characterized in tomato and arabidopsis. in this study, we isolated two putative heatshock factors oshsf6 and oshsf12 that interact specifically with heat-shock element (hse) from oryza sativa l by yeast one-hybrid method. the full-length cdna of oshsf6 and oshsf12 have 1074bp and 920bp open reading frame (orf), respectively. analysis of the deduced amino acid sequences revealed that oshsf6 was a class a heat s ... | 2005 | 16202242 |
| [riboswitches]. | riboswitches are rna structures able to bind small molecules and regulate gene expression at both, transcriptional and translational level. they are present in a wide variety of bacterial species. in bacillus subtilis more than 2% of the genome is regulated by riboswitches. metabolite binding is highly specific and can be provided in cooperative manner. several riboswitches has been identified and characterized to be specific for flavin mononucleotide, thiamine, thiamine pyrophosphate, adenosylc ... | 2005 | 16209348 |
| the oryza map alignment project: the golden path to unlocking the genetic potential of wild rice species. | the wild species of the genus oryza offer enormous potential to make a significant impact on agricultural productivity of the cultivated rice species oryza sativa and oryza glaberrima. to unlock the genetic potential of wild rice we have initiated a project entitled the 'oryza map alignment project' (omap) with the ultimate goal of constructing and aligning bac/stc based physical maps of 11 wild and one cultivated rice species to the international rice genome sequencing project's finished refere ... | 2005 | 16217601 |
| chromosomal regions with quantitative trait loci controlling cadmium concentration in brown rice (oryza sativa). | a novel mapping population consisting of 39 chromosome segment substitution lines (cssls) was used to locate the putative quantitative trait loci (qtls) for cadmium (cd) concentration in brown rice (oryza sativa). the mapping population carried a single chromosome segment of 'kasalath' (indica) in each line overlapping with neighbouring segments in a 'koshihikari' (japonica) genetic background. the parents and cssls were grown in pots filled with cd-polluted soil until grain filling. the brown r ... | 2005 | 16219074 |
| loci controlling partial resistance to rice blast do not show marked qtl x environment interaction when plant nitrogen status alters disease severity. | plant disease susceptibility is often increased by nitrogen (n) application. therefore, it is important to know if resistance loci are effective in different plant n environments. one-hundred lines of the bala x azucena rice (oryza sativa) mapping population were grown in two n treatments and tested for partial resistance to blast (magnaporthe grisea) isolate cd100. disease severity (ds), the number and size of lesions and plant n and c concentrations were measured and the results subject to qua ... | 2005 | 16219084 |
| plant-derived transfer dnas. | the transfer of dna from agrobacterium to plant cell nuclei is initiated by a cleavage reaction within the 25-bp right border of ti plasmids. in an effort to develop all-native dna transformation vectors, 50 putative right border alternatives were identified in both plant expressed sequence tags and genomic dna. efficacy tests in a tobacco (nicotiana tabacum) model system demonstrated that 14 of these elements displayed at least 50% of the activity of conventional agrobacterium transfer dna bord ... | 2005 | 16244143 |
| improvement of human lysozyme expression in transgenic rice grain by combining wheat (triticum aestivum) puroindoline b and rice (oryza sativa) gt1 promoters and signal peptides. | heterologous protein expression levels in transgenic plants are of critical importance in the production of plant-made pharmaceuticals (pmps). we studied a puroindoline b promoter and signal peptide (tapur) driving human lysozyme expression in rice endosperm. the results demonstrated that human lysozyme expressed under the control of the tapur cassette is seed-specific, readily extractable, active, and properly processed. immuno-electron microscopy indicated that lysozyme expressed from this cas ... | 2005 | 16245149 |
| transgenic rice plants expressing human cyp1a1 remediate the triazine herbicides atrazine and simazine. | the human cytochrome p450 cyp1a1 gene was introduced into rice plants (oryza sativa cv. nipponbare). one-month-old cyp1a1 plants grown in soil clearly showed a healthy growth and tolerance to 8.8 microm atrazine and 50 microm simazine, but nontransgenic plants were completely killed by the herbicides. although transgenic and nontransgenic plants metabolized the two herbicides into the same sets of compounds, cyp1a1 plants metabolized atrazine and simazine more rapidly than did control plants. in ... | 2005 | 16248553 |
| multigenic families and proteomics: extended protein characterization as a tool for paralog gene identification. | in classical proteomic studies, the searches in protein databases lead mostly to the identification of protein functions by homology due to the non-exhaustiveness of the protein databases. the quality of the identification depends on the studied organism, its complexity and its representation in the protein databases. nevertheless, this basic function identification is insufficient for certain applications namely for the development of rna-based gene-silencing strategies, commonly termed rna int ... | 2005 | 15627959 |
| inhibitory effects of a rice hull constituent on tumor necrosis factor alpha, prostaglandin e2, and cyclooxygenase-2 production in lipopolysaccharide-activated mouse macrophages. | isovitexin, isolated from rice hull of oryza sativa, has been characterized as a potent antioxidant. its antioxidant activity, determined on the basis of inhibition of lipid peroxidation by the fenton reaction, was comparable with that of alpha-tocopherol, a well-established antioxidant. isovitexin was able to reduce the amount of hydrogen peroxide production induced by lipopolysaccharide (lps) in mouse macrophage raw264.7 cells. in this study, we assessed its effects on the production of tumor ... | 2005 | 15965085 |
| biological and molecular variability of sarocladium oryzae, the sheath rot pathogen of rice (oryza sativa l.). | sheath rot disease of rice caused by sarocladium oryzae (sawada) (=acrocylindrium oryzae, sawada) has become an important production constraint in all rice-growing countries. pathogenicity, phytotoxic metabolites, and random amplified polymorphic dna (rapd) markers were used to assess the level of genetic variability of s. oryzae derived from rice cultivars, cr1018, ir36, and ir50, of different locations in north east and south india. variability in pathogenicity, phytotoxic metabolite productio ... | 2005 | 15968500 |
| maintaining a conserved methylation in plant and insect u2 snrna through compensatory mutation by nucleotide insertion. | the extensive post-transcriptional modification of u2 snrna is required for spliceosome assembly and pre-mrna splicing in vertebrates. however, the rare modification of u2 snrna in yeast implies a different mechanism for regulating spliceosome biogenesis in single-celled eukaryotes. to understand the evolutionary pattern of u2 snrna methylation, we determined for the first time, the 2'-o-methylations of u2 snrna in oryza sativa, arabidopsis thaliana and drosophila melanogaster, and revealed two ... | 2005 | 16223710 |
| high-resolution mapping, cloning and molecular characterization of the pi-k ( h ) gene of rice, which confers resistance to magnaporthe grisea. | in order to understand the molecular mechanisms involved in the gene-for-gene type of pathogen resistance, high-resolution genetic and physical mapping of resistance loci is required to facilitate map-based cloning of resistance genes. here, we report the molecular mapping and cloning of a dominant gene (pi-k ( h )) present in the rice line tetep, which is associated with resistance to rice blast disease caused by magnaporthe grisea. this gene is effective against m. grisea populations prevalent ... | 2005 | 16228246 |
| osgap1 functions as a positive regulator of osrab11-mediated tgn to pm or vacuole trafficking. | the ypt/rab family of small g-proteins is important in regulating vesicular transport. rabs hydrolyze gtp very slowly on their own and require gtpase-activating proteins (gaps). here we report the identification and characterization of osgap1, a rab-specific rice gap. osgap1 strongly stimulated osrab8a and osrab11, which are homologs of the mammalian rab8 and rab11 proteins that are essential for golgi to plasma membrane (pm) and trans-golgi network (tgn) to pm trafficking, respectively. substit ... | 2005 | 16230331 |
| [ssr markers linked with early stability in rice]. | one hundred and thirty hybrids derived from the crosses between nine rice strains stabilized in early generation and seven cultivars (oryza sativa l.). in their f2 populations, 32 uniform strains of different agronomic traits were observed. in the same combination of these uniform strains,there were strains segregating in the mendelian manner. ssr markers analysis showed that f2 and f3 populations of the uniform strains and their f1 plants displayed the same markers indicating all the uniform st ... | 2005 | 16231739 |
| plasmid-associated genes in the model micro-symbiont sinorhizobium meliloti 1021 affect the growth and development of young rice seedlings. | sinorhizobium meliloti strain 1021 and its closely related strain rm2011 inhibit rice seedling (oryza sativa l. cv. pelde) growth and development under certain rice-growing conditions. experiments showed that inoculation of seedlings with approximately less than 10 cells of 1021 was sufficient to cause this inhibition. by using a series of plasmid-cured and plasmid-deleted derivatives of rm2011, it was found that interactions between genes encoded on psyma, and possibly psymb, of rm2011, affecte ... | 2005 | 16232297 |
| competition among symbiotic cyanobacterial nostoc strains forming artificial associations with rice (oryza sativa). | competition among four symbiotically competent nostoc strains, colonizing rice roots, was examined using hetr-dgge (denaturing gradient gel electrophoresis) as strain identification. although mixed in various combinations, only one strain at a time associated with the rice roots. nostoc strain 8964:3 was the most competitive and our data suggest that its competitive fitness was dependent on rapid hormogonial spreading as displayed on agar plates. furthermore, rice roots induced hormogonia in all ... | 2005 | 15796991 |
| rapid and selective surveillance of arabidopsis thaliana genome annotations with centrifuge. | centrifuge is a user-friendly system to simultaneously access arabidopsis gene annotations and intra- and inter-organism sequence comparison data. the tool allows rapid retrieval of user-selected data for each annotated arabidopsis gene providing, in any combination, data on the following features: predicted protein properties such as mass, pi, cellular location and transmembrane domains; swiss-prot annotations; interpro domains; gene ontology records; verified transcription; blast matches to th ... | 2005 | 15817689 |
| [inducible expression of a promoter of the gene encoding barley beta-1, 3-glucanase isoenzyme giii in transgenic rice]. | a promoter of the gene encoding beta-1, 3-glucanase isoenzyme giii was amplified from barley genomic dna using pcr. the giii gene promoter, designated p(giii), was ligated upstream of the gus report gene and pgiii-gus fusion fragment was then cloned into a binary vector pcambia1300 for agrobacterium-mediated transformation of rice (oryza sativa l. cv. taipei 309). pcr analyses indicated that the fusion gene was present in all t0 transgenic plants. the integration of the gene into rice genomic dn ... | 2005 | 15840932 |
| functional characterization and expression analysis of a gene, osent2, encoding an equilibrative nucleoside transporter in rice suggest a function in cytokinin transport. | we identified four genes for potential equilibrative nucleoside transporters (ents) from rice (oryza sativa; designated osent1 through osent4). growth analysis of budding yeast (saccharomyces cerevisiae) cells expressing osents showed that osent2 transported adenosine and uridine with high affinity (adenosine, k(m) = 3.0 microm; uridine, k(m) = 0.7 microm). purine or pyrimidine nucleosides and 2'-deoxynucleosides strongly inhibited adenosine transport via osent2, suggesting that osent2 possesses ... | 2005 | 15849298 |
| the lpb1 gene is important for acclimation of chlamydomonas reinhardtii to phosphorus and sulfur deprivation. | organisms exhibit a diverse set of responses when exposed to low-phosphate conditions. some of these responses are specific for phosphorus limitation, including responses that enable cells to efficiently scavenge phosphate from internal and external stores via the production of high-affinity phosphate transporters and the synthesis of intracellular and extracellular phosphatases. other responses are general and occur under a number of different environmental stresses, helping coordinate cellular ... | 2005 | 15849300 |
| [analysis of paddy field evapotranspiration in north china and calculation of crop coefficient]. | the potential evapotranspiration (et0) values from 1951 to 2002 for the lower reaches of liaohe plain, north china, were estimated by penman-monteith equation, and the annual change in et0 was analyzed. by using non-weighting lysimeters at shenyang experimental station of ecology (cas) in north china, the daily evapotranspiration of flooded rice (oryza sativa l.) at the interval of 10-11 days was determined for eight seasons from 1993-2002 (except for 1997 and 1999). the results showed that the ... | 2005 | 15852960 |
| transgenic rice containing human cyp2b6 detoxifies various classes of herbicides. | the human gene for cyp2b6, a cytochrome p450 monooxygenase that inactivates xenobiotic chemicals, was introduced into oryza sativa cv. nipponbare by agrobacterium-mediated transformation. at germination, r(1) seeds of transgenic rice plants expressing cyp2b6 (cyp2b6 rice) showed a high tolerance to 5 microm metolachlor, a preemergence herbicide that is degraded by cyp2b6. thin-layer chromatography after culture with (14)c-labeled metolachlor revealed that the amounts of residual metolachlor decr ... | 2005 | 15853388 |
| pleomorphomonas oryzae gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from paddy soil of oryza sativa. | five strains of free-living diazotrophs isolated from rice were characterized by using a polyphasic approach. the strains were found to be very closely related, with 99-100 % 16s rrna gene sequence similarity and dna-dna hybridization values greater than 70 %, suggesting that they represent a single species. when compared with other recognized species, they showed not more than 93 and 89 % similarity for the 16s rrna and nifh gene sequences, respectively. phylogenetic distances showed that these ... | 2005 | 15879261 |
| cloning and expression of a farnesyl diphosphate synthase in centella asiatica (l.) urban. | a cdna encoding farnesyl diphosphate synthase (fps; ec2.5.1.1/ec2.5.1.10) was isolated from centella asiacita (l.) urban, using degenerate primers based on two highly conserved domains. a full-length cdna clone was subsequently isolated by rapid amplification of cdna ends (race) pcr. the sequence of the cafps (c. asiatica farnesyl diphosphate synthase) cdna contains an open reading frame of 1029 nucleotides encoding 343 amino acids with a molecular mass of 39.6 kda. the deduced cafps amino acid ... | 2005 | 15879717 |
| comparison of the yeast proteome to other fungal genomes to find core fungal genes. | the purpose of this research was to search for evolutionarily conserved fungal sequences to test the hypothesis that fungi have a set of core genes that are not found in other organisms, as these genes may indicate what makes fungi different from other organisms. by comparing 6355 predicted or known yeast (saccharomyces cerevisiae) genes to the genomes of 13 other fungi using standalone tblastn at an e-value <1e-5, a list of 3340 yeast genes was obtained with homologs present in at least 12 of 1 ... | 2005 | 15883882 |
| large-scale identification of expressed sequence tags involved in rice and rice blast fungus interaction. | to better understand the molecular basis of the defense response against the rice blast fungus (magnaporthe grisea), a large-scale expressed sequence tag (est) sequencing approach was used to identify genes involved in the early infection stages in rice (oryza sativa). six cdna libraries were constructed using infected leaf tissues harvested from 6 conditions: resistant, partially resistant, and susceptible reactions at both 6 and 24 h after inoculation. two additional libraries were constructed ... | 2005 | 15888683 |
| crystal structure of nucleoside diphosphate kinase required for coleoptile elongation in rice (oryza sativa l.). | nucleoside diphosphate kinase (ndk) is a ubiquitous enzyme found in all organisms and cell types, and catalyzes the transfer of the phosphoryl group from a nucleoside triphosphate to a nucleoside diphosphate. the enzyme is involved in and required for coleoptile elongation in rice as the level of the rice ndk (rndk) changes during seed germination and the early stages of seedling growth. the expression of rice ndk gene is up-regulated in the growing coleoptiles when the anaerobic stress persists ... | 2005 | 15890279 |
| purification and characterization of a xip-type endoxylanase inhibitor from rice (oryza sativa). | a rice xip-type inhibitor was purified by affinity chromatography with an immobilized aspergillus aculeatus family 10 endoxylanase. rice xip is a monomeric protein, with a molecular mass of ca. 32 kda and a pi of ca. 5.6. its n-terminal amino acid sequence was identical to that of a rice chitinase homologue, demonstrating the difficulty when using sequence information to differentiate between endoxylanase inhibitors and (putative) chitinases in rice. rice xip inhibited different endoxylanases to ... | 2005 | 15895691 |
| effects of cracks and some key factors on emissions of nitrous oxide in paddy fields. | paddy field is a primary agricultural landscape in the south of china and is often regarded as one of main sources emitting nitrous oxide to atmosphere. the nitrous oxide emissions under a variety of paddy field practices, such as fertilization, flooding/draining management were investigated to study on agricultural activities on paddy field affect the dynamic process of the emission. under no addition of fertilizers the average emission flux of nitrous oxide was 8.55 microg/(m2 x h) during the ... | 2005 | 15900754 |
| enhanced drought tolerance of transgenic rice plants expressing a pea manganese superoxide dismutase. | we investigated the role that manganese superoxide dismutase (mnsod), an important antioxidant enzyme, may play in the drought tolerance of rice. mnsod from pea (pisum sativum) under the control of an oxidative stress-inducible swpa2 promoter was introduced into chloroplasts of rice (oryza sativa) by agrobacterium-mediated transformation to develop drought-tolerant rice plants. functional expression of the pea mnsod in transgenic rice plants (t1) was revealed under drought stress induced by poly ... | 2005 | 15900889 |
| refolding and purification of recombinant osnifu1a domain ii that was expressed by escherichia coli. | osnifu1a is a nifu-like rice (oryza sativa) protein, discovered recently. its amino acid sequence is very homologous to the sequence of cyanobacterial cnfu and to the sequences of nifu c-terminal domains. based on its sequence, osnifu1a is probably a modular structure consisting of two cnfu-like domains, with domain i (formed by residues leu73 to gly153) and domain ii (formed by residues leu154 to ser226). domain i have a conserved cys-x-x-cys motif, which may function as an iron-sulfur cluster ... | 2005 | 15978837 |
| a gibberellin-regulated calcineurin b in rice localizes to the tonoplast and is implicated in vacuole function. | many developmental and environmental signals are transduced through changes in intracellular calcium concentrations, yet only a few calcium-binding proteins have been identified in plants. calcineurin b-like (cbl) proteins are calcium-binding proteins that are thought to function as plant signal transduction elements. rna profiling using a rice (oryza sativa cv nipponbare) oligonucleotide microarray was used to monitor gene expression in de-embryonated rice grains. this analysis showed that a pu ... | 2005 | 15980189 |
| interacting proteins and differences in nuclear transport reveal specific functions for the nap1 family proteins in plants. | nucleosome assembly protein 1 (nap1) is conserved from yeast to human and facilitates the in vitro assembly of nucleosomes as a histone chaperone. inconsistent with their proposed function in the nucleus, however, many nap1 proteins had been reported to localize in the cytoplasm. we investigated the subcellular localization of tobacco (nicotiana tabacum) and rice (oryza sativa) nap1 family proteins first by identification of interacting partners and by direct examination of the localization of g ... | 2005 | 15980199 |
| structure, evolution, and expression of the two invertase gene families of rice. | invertases catalyze the irreversible hydrolysis of sucrose to glucose and fructose. plants contain two unrelated families of these enzymes: acid forms that derive from periplasmic invertases of eubacteria and are found in cell wall and vacuole, and neutral/alkaline forms evolved from the cytosolic invertases of cyanobacteria. genomes of rice (oryza sativa) and thale cress (arabidopsis thaliana) contain multiple genes encoding these two families. here for rice we identify the member genes of a ce ... | 2005 | 15983871 |
| distribution of fertility-restorer genes for wild-abortive and honglian cms lines of rice in the aa genome species of genus oryza. | rice (oryza sativa) is one of the most important cereal plants in the world. wild-abortive (wa) and honglian (hl) cytoplasmic male sterility (cms) have been used extensively in the production of hybrid seeds. although a variable number of fertility-restorer genes (rf) for wa and hl-cms have been identified in various cultivars, information on rf in oryza species with the aa-genome is sparse. therefore the distribution and heredity of rf for wa and hl-cms in wild rice species of oryza with the aa ... | 2005 | 15987696 |
| evolutionary conservation of minor u12-type spliceosome between plants and humans. | splicing of rare, u12-type or at-ac introns is mediated by a distinct spliceosome that assembles from u11, u12, u4atac, u6atac, and u5 snrnps. although in human cells the protein composition of minor and major snrnps is similar, differences, particularly in u11 and u12 snrnps, have been recently described. we have identified an arabidopsis u11 snrnp-specific 35k protein as an interacting partner of an rs-domain-containing cyclophilin. by using a transient expression system in arabidopsis protopl ... | 2005 | 15987817 |
| mechanistic information from analysis of molecular weight distributions of starch. | a methodology is developed for interpreting the molecular weight distributions of debranched amylopectin, based on techniques developed for quantitatively and qualitatively finding mechanistic information from the molecular weight distributions of synthetic polymers. if the only events occurring are random chain growth and stoppage (i.e., the rates are independent of degree of polymerization over the range in question), then the number of chains of degree of polymerization n, p(n), is linear in ... | 2005 | 16004469 |
| munich information center for protein sequences plant genome resources: a framework for integrative and comparative analyses 1(w). | with several plant genomes sequenced, the power of comparative genome analysis can now be applied. however, genome-scale cross-species analyses are limited by the effort for data integration. to develop an integrated cross-species plant genome resource, we maintain comprehensive databases for model plant genomes, including arabidopsis (arabidopsis thaliana), maize (zea mays), medicago truncatula, and rice (oryza sativa). integration of data and resources is emphasized, both in house as well as w ... | 2005 | 16010004 |
| [eco-toxicological effects of selenium and its critical value on oryza sativa]. | in a pot experiment with brown-yellow soil, this paper studied the effects of different concentration selenium on the growth and activated oxygen- scavenging system of oryza sativa. the results showed that low concentration se (< 8 mg x kg(-1)) had a promoting effect on the wheat biomass, chlorophyll a, chlorophyll a/b, and activated oxygen- scavenging system, while high concentration se (> 16 mg x kg(-1)) was in adverse. soil se concentration had a significant correlation with root biomass, sho ... | 2005 | 16011166 |
| [stress-resistance of weedy rice luolijing (oryza sativa)]. | weedy rice luolijing (oryza sativa) is generated in dandong, liaoning province of northeast china. its plant height is taller than normal planted rice varieties, and its caryopsis is medium or long in size, which dropped easily after matured. the color of the caryopsis shell is straw-like or yellow alternated with black-gray. the spikelet is with or without awns, and the length of the awns is 2-12 cm. the 1000 caryopsis weight is 23.5 g, and the hull is nacarat. the seed germination rate at 13-3 ... | 2005 | 16011174 |
| azospirillum oryzae sp. nov., a nitrogen-fixing bacterium isolated from the roots of the rice plant oryza sativa. | the taxonomic position of the free-living diazotrophic strain coc8(t) isolated from rice was investigated based on phylogenetic analyses. 16s rrna gene sequence analyses indicated that strain coc8(t) was closely related to the genus azospirillum (96% similarity). chemotaxonomic characteristics (g+c content of the dna 66.8 mol%, q-10 quinone system, 18:1omega7c as the major fatty acid and 14:0 3-oh and 16:0 3-oh as the major hydroxy fatty acids) were also similar to those of the genus azospirillu ... | 2005 | 16014463 |
| identification of 33 rice aquaporin genes and analysis of their expression and function. | plant aquaporins form a large protein family including plasma membrane-type (pips) and tonoplast-type aquaporins (tips), and facilitate osmotic water transport across membranes as a key physiological function. we identified 33 genes for aquaporins in the genome sequence of rice (oryza sativa l. cv. nipponbare). we investigated their expression levels in leaf blades, roots and anthers of rice (cv. akitakomachi) using semi-quantitative reverse transcription-pcr (rt-pcr). at both early tillering (2 ... | 2005 | 16033806 |
| structure-based in vitro engineering of the anthranilate synthase, a metabolic key enzyme in the plant tryptophan pathway. | rice (oryza sativa) anthranilate synthase alpha-subunit, oasa2, was modified by in vitro mutagenesis based on structural information from bacterial homologs. twenty-four amino acid residues, predicted as putative tryptophan binding sites or their proximal regions in the oasa2 sequence, were selected and 36 mutant oasa2 genes were constructed by pcr-based site-directed mutagenesis. corresponding mutant proteins were synthesized in a combination of two in vitro systems, transcription with a bacter ... | 2005 | 16040654 |
| lip19, a basic region leucine zipper protein, is a fos-like molecular switch in the cold signaling of rice plants. | the rice low-temperature-induced lip19 gene encodes a 148-amino-acid basic region/leucine zipper (bzip) protein, termed lip19. in this study we characterized lip19 and showed that it lacks the usual ability of bzip proteins to homodimerize and to bind dna, as does the fos protein in mammals. using a yeast two-hybrid system, the cdna clones whose products interact with lip19 were screened. this search revealed a clone termed osobf1 (oryza sativa obf1) that encodes a new bzip protein (osobf1). thi ... | 2005 | 16051676 |
| sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species. | rice (oryza sativa l.) chromosome 3 is evolutionarily conserved across the cultivated cereals and shares large blocks of synteny with maize and sorghum, which diverged from rice more than 50 million years ago. to begin to completely understand this chromosome, we sequenced, finished, and annotated 36.1 mb ( approximately 97%) from o. sativa subsp. japonica cv nipponbare. annotation features of the chromosome include 5915 genes, of which 913 are related to transposable elements. a putative functi ... | 2005 | 16109971 |
| interaction of arsenic with zinc and organics in a rice (oryza sativa l.)--cultivated field in india. | a laboratory experiment on an inceptisol with ph 7.6, organic carbon 6.8 g kg(-1), and 0.5 m nahco3 extractable arsenic 0.4 mg kg(-1) was conducted to study the interaction effect of graded levels of arsenic (0, 5, and 10 mg kg(-1)) with zinc (0, 10, and 20 mg kg(-1)) and organics (0, 1, and 2% on soil weight basis) separately on the mobilization of arsenic in soils. the results show that the amount of 0.5 m nahco3 extractable arsenic at ph 8.5 increased with the progress of submergence up to 35 ... | 2005 | 16113941 |
| molecular cloning and characterization of oryzacystatin-iii, a novel member of phytocystatin in rice (oryza sativa l. japonica). | on the basis of cdna sequences, we found that the calli of rice encodes an amino acid sequence that shares 56% and 89% identity, respectively, with oryzacystatin-i and oryzacystatin-ii. this sequence differs from that of oryzacystatin-ii in the n-terminal region (gln(7)-ala(19) in the oryzacystatin-iii numbering), and this region contained a glycine residue (gly(14)), which is evolutionarily conserved in the cystatin superfamily. we named this novel protein oryzacystatin-iii. nucleotide sequenci ... | 2005 | 15969500 |
| phytoremediation of metolachlor by transgenic rice plants expressing human cyp2b6. | we introduced the human cytochrome p450 gene cyp2b6 into rice plants (oryza sativa l. cv. nipponbare), and the cyp2b6-expressing rice plants became more tolerant to various herbicides than nontransgenic nipponbare rice plants. in particular, cyp2b6 rice plants grown in soil showed tolerance to the chloroacetanilide herbicides alachlor and metolachlor. we evaluated the degradation of metolachlor by cyp2b6 rice plants to confirm the metabolic activity of the introduced cyp2b6. although both cyp2b6 ... | 2005 | 16277416 |
| [effects of rice-duck farming system on oryza sativa growth and its yield]. | the study showed that under rice-duck farming, the number of rice non-productive tiller reduced significantly, the ratio of its effective panicles increased by 8.08 %, and its basal penetration light rate enhanced by 4.05%. at full-heading and maturing stages, the green leaf ratio under rice-duck farming was 6.01% and 10.65% higher than the control, and the leaf chlorophyll content was increased by 2.90% and 17.82%, respectively. under rice-duck farming, the root vigour at full-heading stage and ... | 2005 | 16252861 |
| cloning and biochemical properties of cdpk gene oscdpk14 from rice. | a rice cdpk gene, oscdpk14 (ay144497), was cloned from developing caryopses of rice (oryza sativa cv. zhonghua 15). its cdna sequence (1922 bp) contains an orf encoding a 514 amino acids protein (56.7kd, pl 5.18). oscdpk14 shows the typical structural features of the cdpk family, including a conserved catalytic ser/thr kinase domain, an autoinhibitory domain and a cam-like domain with four putative ca2+-binding ef hands. subcellular targeting indicated that oscdpk14 was located in the cytoplasm, ... | 2005 | 16255173 |
| oszip4, a novel zinc-regulated zinc transporter in rice. | zinc (zn) is an essential element for the normal growth of plants but information is scarce on the mechanisms whereby zn is transported in rice (oryza sativa l.) plants. four distinct genes, oszip4, oszip5, oszip6, and oszip7 that exhibit sequence similarity to the rice ferrous ion transporter, osirt1, were isolated. microarray and northern blot analysis revealed that oszip4 was highly expressed under conditions of zn deficiency in roots and shoots. real-time-pcr revealed that the oszip4 transcr ... | 2005 | 16263903 |
| the rice dwarf virus p2 protein interacts with ent-kaurene oxidases in vivo, leading to reduced biosynthesis of gibberellins and rice dwarf symptoms. | the mechanisms of viral diseases are a major focus of biology. despite intensive investigations, how a plant virus interacts with host factors to cause diseases remains poorly understood. the rice dwarf virus (rdv), a member of the genus phytoreovirus, causes dwarfed growth phenotypes in infected rice (oryza sativa) plants. the outer capsid protein p2 is essential during rdv infection of insects and thus influences transmission of rdv by the insect vector. however, its role during rdv infection ... | 2005 | 16299167 |
| genome-wide identification and molecular characterization of ole_e_i, allerg_1 and allerg_2 domain-containing pollen-allergen-like genes in oryza sativa. | pollen allergens play important roles in plant development in addition to their allergenic nature for human. more than 10 groups of pollen allergens have been reported. among them, pollen_ole_e_i (ole), pollen_allerg_1 (allerg1) and pollen_allerg_2 (allerg2) domain-containing proteins are the majority of allergens. we have identified 114 pollen-allergen-like genes in rice genome by bioinformatics using public databases. among them, 45 genes encode ole domain-containing proteins, 62 with allerg1 ... | 2005 | 16303748 |
| development of simple and efficient in planta transformation method for rice (oryza sativa l.) using agrobacterium tumefaciens. | seeds of rice (oryza sativa l. var. koshihikari) were soaked in water for 2 d. thereafter, the embryo containing an apical meristem was inoculated with agrobacterium tumefaciens by piercing a site of the husk overlying the embryonic apical meristem with a needle that had been dipped in an a. tumefaciens inoculum. the inoculated seeds were then grown to maturation (t0 plants) and allowed to pollinate naturally to set seeds (t1 plants) in pots under nonsterile conditions. to examine the transforma ... | 2005 | 16310727 |
| low frequency of transgene flow from bt/cpti rice to its non-transgenic counterparts planted at close spacing. | crop-to-crop transgene flow will affect seed purity of non-gm rice varieties, leading to unwanted consequences. to assess the maximum probability of transgene outflow in rice (oryza sativa), gene flow experiments were conducted with three cultivation patterns with different mixed-planting proportions of adjacent gm and non-gm rice at two sites in fujian and hainan provinces of china. three gm rice lines containing two insect-resistance genes (bt/cpti) and their non-gm counterparts were used in t ... | 2005 | 16313639 |
| analysis of substrate specificity of pig cyp2b22 and cyp2c49 towards herbicides by transgenic rice plants. | we introduced two novel types of pig (sus scrofa) cytochrome p450, cyp2b22 and cyp2c49, into rice plants (oryza sativa l. cv. 'nipponbare') to produce herbicide-tolerant plants and to confirm the metabolic activities of the cytochrome p450 species. in germination tests, both types of transgenic plants showed tolerance to various herbicides with different modes of action. cyp2b22 rice plants showed tolerance towards 12 herbicides including chlortoluron (100 microm), amiprofos-methyl (2.5 microm), ... | 2005 | 16315095 |
| genealogy of the "green revolution" gene in rice. | during the "green revolution" of rice, high-yielding varieties (hyvs) were developed using a semi-dwarf gene (sd1 or osga20ox2). the presence or absence of the two mutant alleles (dgwg type in dee-geo-woo-gen and jkk type in jikkoku) were surveyed by pcr using 256 accessions of eight wild and two cultivate rice species. the dgwg allele was detected in a landrace (oryza sativa) and two accessions of wild rice (o. rufipogon), all of which are from china, showing their limited distribution. genealo ... | 2005 | 16394586 |
| real-time polymerase chain reaction based assays for quantitative detection of barley, rice, sunflower, and wheat. | quality assurance is a major issue in the food industry. the authenticity of food ingredients and their traceability are required by consumers and authorities. plant species such as barley (hordeum vulgare), rice (oryza sativa), sunflower (helianthus annuus), and wheat (triticum aestivum) are very common among the ingredients of many processed food products; therefore the development of specific assays for their specific detection and quantification are needed. furthermore, the production and tr ... | 2005 | 16131102 |
| isotopic discrimination of zinc in higher plants. | * the extent of isotopic discrimination of transition metals in biological processes is poorly understood but potentially has important applications in plant and biogeochemical studies. * using multicollector inductively coupled plasma (icp) mass spectrometry, we measured isotopic fractionation of zinc (zn) during uptake from nutrient solutions by rice (oryza sativa), lettuce (lactuca sativa) and tomato (lycopersicon esculentum) plants. * for all three species, the roots showed a similar extent ... | 2005 | 15720681 |
| rice serk1 gene positively regulates somatic embryogenesis of cultured cell and host defense response against fungal infection. | here we report on the isolation and characterization of a somatic embryogenesis receptor-like kinase (osserk1) gene in rice (oryza sativa). the osserk1 gene belongs to a small subfamily of receptor-like kinase genes in rice and shares a highly conserved gene structure and extensive sequence homology with previously reported plant serk genes. though it has a basal level of expression in various rice organs/tissues, as high expression level was detected in rice callus during somatic embryogenesis. ... | 2005 | 15968510 |
| calculation of binding length of base-specific dna dyes by comparison of sequence and flow cytometric data. application to oryza sativa and arabidopsis thaliana. | from biochemical experiments it has been found that at- and gc-specific dyes need a certain number of consecutive bases of the same type for binding one dye molecule. from known base sequences the amount of bases included in dye binding can be calculated and compared with experimental data from flow cytometry. oryza sativa and arabidopsis thaliana are the first higher plants which are nearly completely (>90%) sequenced. from the published sequences the theoretical fluorescence intensity of base- ... | 2005 | 15498596 |
| molecular analysis of the crinkly4 gene family in arabidopsis thaliana. | the maize (zea mays l.) crinkly4 (cr4) gene encodes a serine/threonine receptor-like kinase that controls an array of developmental processes in the plant and endosperm. the arabidopsis thaliana (l.) heynh. genome encodes an ortholog of cr4, acr4, and four crinkly4-related (crr) proteins: atcrr1, atcrr2, atcrr3 and atcrk1. the available genome sequence of rice (oryza sativa l.) encodes a cr4 ortholog, oscr4, and four crr proteins: oscrr1, oscrr2, oscrr3 and oscrr4, not necessarily orthologous to ... | 2005 | 15549374 |
| evolutionary expansion, gene structure, and expression of the rice wall-associated kinase gene family. | the wall-associated kinase (wak) gene family, one of the receptor-like kinase (rlk) gene families in plants, plays important roles in cell expansion, pathogen resistance, and heavy-metal stress tolerance in arabidopsis (arabidopsis thaliana). through a reiterative database search and manual reannotation, we identified 125 oswak gene family members from rice (oryza sativa) japonica cv nipponbare; 37 (approximately 30%) oswaks were corrected/reannotated from earlier automated annotations. of the 1 ... | 2005 | 16286450 |