Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| preferential attack of the (s)-configured ether-linked carbons in bis-(1-chloro-2-propyl) ether by rhodococcus sp. strain dtb. | rhodococcus sp. strain dtb (dsm 44534) was grown on a mixture of (r,r)-, (s,s)- and meso-bis-(1-chloro-2-propyl) ether (bcpe) as the sole source of carbon and energy. during bcpe degradation 1'-chloro-2'-propyl-3-chloro-2-prop-1-enyl-ether (dve), 1-chloro-2-propanol and chloroacetone intermediates were formed. the bcpe or dve stereoisomers were metabolized in consecutive order via scission of the ether bond, with discrimination against the (r) configuration. resting cell suspensions of rhodococc ... | 2006 | 16420620 |
| study of a hexane-degrading consortium in a biofilter and in liquid culture: biodiversity, kinetics and characterization of degrading strains. | a gasoline-degrading consortium, originating from a mexican soil, was used to study its hexane-degradation kinetics in liquid culture and in a biofilter with mineral support. the biodiversity of the consortium depending on the culture conditions and electron and energy source (gasoline, hexane in liquid or hexane in the biofilter) was analyzed using a 16s rrna-based approach. significant differences between the populations were observed, indicating a probable adaptation to the substrate. two str ... | 2006 | 16420632 |
| potential of rhodococcus strains for biotechnological vanillin production from ferulic acid and eugenol. | the potential of two rhodococcus strains for biotechnological vanillin production from ferulic acid and eugenol was investigated. genome sequence data of rhodococcus sp. i24 suggested a coenzyme a-dependent, non-beta-oxidative pathway for ferulic acid bioconversion, which involves feruloyl-coa synthetase (fcs), enoyl-coa hydratase/aldolase (ech), and vanillin dehydrogenase (vdh). this pathway was proven for rhodococcus opacus pd630 by physiological characterization of knockout mutants. however, ... | 2006 | 16421716 |
| sequence analysis of three plasmids harboured in rhodococcus erythropolis strain pr4. | rhodococcus erythropolis strain pr4 has been isolated as an alkane-degrading bacterium. the strain harbours one linear plasmid, prel1 (271 577 bp) and two circular plasmids, prec1 (104 014 bp) and prec2 (3637 bp), all with some sequence similarities to other rhodococcus plasmids. for prel1, prec1 and prec2, 298, 102 and 3 open reading frames, respectively, were predicted. linear plasmid prel1 has several regions homologous to plasmid pbd2 found in r. erythropolis bd2. sequence analysis of prel1 ... | 2006 | 16423019 |
| recurrent rhodococcus equi infection with fatal outcome in an immunocompetent patient. | the majority of human rhodococcus equi infections occur in immunocompromised hosts, especially those with aids, and infection in immunocompetent patients is rare. reported here is a case of r. equi infection in a seemingly healthy patient with a very complicated course. despite neurosurgery and prolonged antibiotic therapy the patient deceased. | 2006 | 16424975 |
| intracranial abscess caused by rhodococcus equi infection in a foal. | a 4-month-old missouri fox trotter colt was examined for a 5-week history of head tilt after treatment for suspected pulmonary rhodococcus equi infection. | 2006 | 16426201 |
| enhanced biodegradation of diesel oil by a newly identified rhodococcus baikonurensis en3 in the presence of mycolic acid. | the aim of the present study was to isolate and characterize a bacterium, strain en3, capable of using diesel oil as a major carbon and energy source, and to analyse the enhancement of diesel oil degradation by this organism using synthetic mycolic acid (2-hexyl-3-hydroxyldecanoic acid). | 2006 | 16430509 |
| resolution of methyl nonactate by rhodococcus erythropolis under aerobic and anaerobic conditions. | [reaction: see text]. an efficient resolution of methyl nonactate is reported by biotransformation in shake flask cultures of rhodococcus erythropolis. the equilibrium of the reaction redox system can be manipulated by switching from aerobic to anaerobic growth, thereby generating both enantiomers of the target in excellent yield and enantiomeric purity. | 2006 | 16435855 |
| structural analysis of an extracellular polysaccharide produced by a benzene tolerant bacterium, rhodococcus sp. 33. | rhodococcus sp. 33 can tolerate and efficiently degrade various concentrations of benzene, one of the most toxic and prevailing environmental pollutants. this strain produces a large quantity of extracellular polysaccharide (33 eps), which plays an important role in the benzene tolerance in rhodococcus sp. 33, especially by helping the cells to survive an initial challenge with benzene. this eps has been reported to be composed of d-galactose, d-glucose, d-mannose, d-glucuronic acid, and pyruvic ... | 2006 | 16442512 |
| antibacterial activity of cefquinome against equine bacterial pathogens. | cefquinome is known for its use as an antibacterial drug in cattle and pigs. the objective of this study was to evaluate the antibacterial activity of cefquinome against equine pathogenic bacteria. the minimum inhibitory concentration (mic) of cefquinome was determined for a total of 205 strains, which had recently been isolated in europe from diseased horses (respiratory infection, foal septicaemia). the bactericidal activity was tested against 19 strains using the time killing method. the post ... | 2006 | 16455213 |
| molecular cloning and expression of genes encoding a novel dioxygenase involved in low- and high-molecular-weight polycyclic aromatic hydrocarbon degradation in mycobacterium vanbaalenii pyr-1. | mycobacterium vanbaalenii pyr-1 is able to metabolize a wide range of low- and high-molecular-weight (hmw) polycyclic aromatic hydrocarbons (pahs). a 20-kda protein was upregulated in pah-metabolizing m. vanbaalenii pyr-1 cells compared to control cultures. the differentially expressed protein was identified as a beta subunit of the terminal dioxygenase using mass spectrometry. pcr with degenerate primers designed based on de novo sequenced peptides and a series of plaque hybridizations were don ... | 2006 | 16461648 |
| mycobacterium tuberculosis prcba genes encode a gated proteasome with broad oligopeptide specificity. | genes predicted to be associated with the putative proteasome of mycobacterium tuberculosis (mtb) play a critical role in defence of the bacillus against nitrosative stress. however, proteasomes are uncommon in eubacteria and it remains to be established whether mtb's prcba genes in fact encode a proteasome. we found that coexpression of recombinant prcb and prca in escherichia coli over a prolonged period at 37 degrees c allowed formation of an alpha(7)beta(7)beta(7)alpha(7), 750 kda cylindrica ... | 2006 | 16468985 |
| j1 acylase, a glutaryl-7-aminocephalosporanic acid acylase from bacillus laterosporus j1, is a member of the alpha/beta-hydrolase fold superfamily. | j1 acylase, a glutaryl-7-aminocephalosporanic acid acylase (gca) isolated from bacillus laterosporus j1, has been conventionally grouped as the only member of class v gca, although its amino acid sequence shares less than 10% identity with members of other classes of gca. instead, it shows higher sequence similarities with rhodococcus sp. strain mb1 cocaine esterase (rhcoce) and acetobacter turbidans alpha-amino acid ester hydrolase (ataeh), members of the alpha/beta-hydrolase fold superfamily. ... | 2006 | 16469317 |
| growth rate and nutrient limitation affect the transport of rhodococcus sp. strain dn22 through sand. | rhodococcus strain dn22 grows on the nitramine explosive rdx as a sole nitrogen source, and is potentially useful for bioremediation of explosives-contaminated soil. in order for strain dn22 to be effectively applied in situ, inoculum cells must reach zones of rdx contamination via passive transport, a process that is difficult to predict at field-scale. we examined the effect of growth conditions on the transport of dn22 cells through sand columns, using chemostat-grown cultures. strain dn22 fo ... | 2006 | 16477351 |
| microbial succession in a compost-packed biofilter treating benzene-contaminated air. | air artificially contaminated with increasing concentrations of benzene was treated in a laboratory scale compost-packed biofilter for 240 days with a removal efficiency of 81-100%. the bacterial community in the packing material (pm) at different heights of the biofilter was analysed every 60 days. bacterial plate counts and ribosomal intergenic spacer analysis (risa) of the isolated strains showed that the number of cultivable aerobic heterotrophic bacteria and the species diversity increased ... | 2006 | 16502043 |
| biological degradation of aflatoxin b1 by rhodococcus erythropolis cultures. | aflatoxin contamination of food and grain poses a serious economic and health problem worldwide, but particularly in africa. aflatoxin b(1) (afb(1)) is extremely mutagenic, toxic and a potent carcinogen to both humans and livestock and chronic exposure to low levels of afb(1) is a concern. in this study, the biodegradation of aflatoxin b(1) (afb(1)) by rhodococcus erythropolis was examined in liquid cultures using thin layer chromatography (tlc), high performance liquid chromatography (hplc), el ... | 2006 | 16504326 |
| crystallization and preliminary x-ray analysis of a bacterial l-amino-acid oxidase from rhodococcus opacus. | l-amino-acid oxidases (ec 1.4.3.2) catalyse the stereospecific oxidative deamination of an l-amino-acid substrate to an alpha-keto acid with the production of ammonia and hydrogen peroxide. in this study, the crystallization and preliminary x-ray analysis of a bacterial l-amino-acid oxidase from rhodococcus opacus (rolaao) is described. rolaao is a dimeric protein consisting of two identical subunits of 489 amino acids with a calculated molecular weight of 54.2 kda and a non-covalently bound fad ... | 2006 | 16511322 |
| bioremediation of hydrocarbon-contaminated polar soils. | bioremediation is increasingly viewed as an appropriate remediation technology for hydrocarbon-contaminated polar soils. as for all soils, the successful application of bioremediation depends on appropriate biodegradative microbes and environmental conditions in situ. laboratory studies have confirmed that hydrocarbon-degrading bacteria typically assigned to the genera rhodococcus, sphingomonas or pseudomonas are present in contaminated polar soils. however, as indicated by the persistence of sp ... | 2006 | 16514512 |
| antimicrobial activity of gallium against virulent rhodococcus equiin vitro and in vivo. | rhodococcus equi, a facultative intracellular bacterium, causes severe pneumonia in foals. evidence suggests that most foals become infected very early in life, when they have immature or ineffective innate immune responses. this study evaluated the antimicrobial activity of gallium against r. equi, as a potential chemoprophylactic and therapeutic agent. rhodococcus equi was grown in media with various concentrations of gallium nitrate (gn), with and without excess iron. gn significantly inhibit ... | 2006 | 16515666 |
| selective biodegradation of s and n heterocycles by a recombinant rhodococcus erythropolis strain containing carbazole dioxygenase. | the carbazole dioxygenase genes were introduced into a dibenzothiophene degrader. the recombinant rhodococcus erythropolis sn8 was capable of efficiently degrading dibenzothiophene and carbazole simultaneously. sn8 could also degrade various alkylated derivatives of carbazole and dibenzothiophene in fs4800 crude oil by just a one-step bioprocess. | 2006 | 16517679 |
| biofilm development of the polyethylene-degrading bacterium rhodococcus ruber. | we have recently isolated a biofilm-producing strain (c208) of rhodococcus ruber that degraded polyethylene at a rate of 0.86% per week (r2=0.98). strain c208 adheres to polyethylene immediately upon exposure to the polyolefin. this initial biofilm differentiates (in a stepwise process that lasts about 20 h) into cell-aggregation-forming microcolonies. further organization yields "mushroom-like" three-dimensional structures on the mature biofilm. the ratio between the population densities of the ... | 2006 | 16534612 |
| characterization of four rhodococcus alcohol dehydrogenase genes responsible for the oxidation of aromatic alcohols. | four genes were isolated and characterized for alcohol dehydrogenases (adhs) catalyzing the oxidation of aromatic alcohols such as benzyl alcohol to their corresponding aldehydes, one from o-xylene-degrading rhodococcus opacus tkn14 and the other three from n-alkane-degrading rhodococcus erythropolis pr4. various aromatic alcohols were bioconverted to their corresponding carboxylic acids using escherichia coli cells expressing each of the four adh genes together with an aromatic aldehyde dehydro ... | 2006 | 16292529 |
| fermentation reactor coupled with capillary electrophoresis for on-line bioprocess monitoring. | in this paper, a filter probe integrated into a computerized pneumatic sampler for capillary electroseparations was developed for an on-line monitoring of bioprocesses. the optimization of the performance of the coupled system was done by using a response surface modeling and three-level two-factor design. the resolution was found to be the most important parameter influencing the performance of an on-line analysis of the microbial metabolism. for the on-line analysis the migration time and dete ... | 2006 | 16495032 |
| transformation of the herbicide 2,6-dichlorobenzonitrile to the persistent metabolite 2,6-dichlorobenzamide (bam) by soil bacteria known to harbour nitrile hydratase or nitrilase. | in soil the herbicide 2,6-dichlorobenzonitrile (dichlobenil) is degraded to the persistent metabolite 2,6-dichlorobenzamide (bam) which has been detected in 19% of samples taken from danish groundwater. we tested if common soil bacteria harbouring nitrile-degrading enzymes, nitrile hydratases or nitrilases, were able to degrade dichlobenil in vitro. we showed that several strains degraded dichlobenil stoichiometrically to bam in 1.5-6.0 days; formation of the amide intermediate thus showed nitri ... | 2006 | 16496093 |
| two cases of equine abortion caused by rhodococcus equi. | rhodococcus equi was isolated from lung, liver, spleen, and stomach content of two aborted equine fetuses of 7 and 8 months gestation from two different farms. lesions included diffuse pyogranulomatous pneumonia with numerous gram-positive coccobacilli within the cytoplasm of macrophages, multinucleated langhans giant cells and neutrophils, and enhanced extramedullary hematopoiesis with megakaryocytosis within the liver and spleen. detection of r. equi was made by bacteriology and immunohistoche ... | 2006 | 16537942 |
| endobacteria in some ectomycorrhiza of scots pine (pinus sylvestris). | the diversity of cultivable endobacteria associated with four different ectomycorrhizal morphotypes (suillus flavidus, suillus variegatus, russula paludosa and russula sp.) of scots pine (pinus sylvestris) was analysed by restriction fragment length polymorphism profiling of pcr-amplified rdna intergenic spacer regions and by sequence analyses of 16s rrna genes. ectomycorrhizal root tip surface-sterilization methods were developed and assessed for their efficiencies. bacterial communities from s ... | 2006 | 16542403 |
| the degradation of n-hexadecane in soil by thermophilic geobacilli. | soil microcosms have been used to demonstrate the ability of indigenous soil thermophiles to degrade effectively a representative alkane (n-hexadecane). a fragment of the alkane mono-oxygenase gene (alkb) was amplified from thermophilic geobacillus thermoleovorans strain t70 by pcr using degenerate primers. the amplicon demonstrated 96% sequence similarity with the alkb gene from rhodococcus erythropolis. critical controls ensured that the positive pcr signal detected was not a result of mesophi ... | 2006 | 16542404 |
| proteomic and transcriptional characterization of aromatic degradation pathways in rhodoccocus sp. strain tfb. | rhodococcus sp. strain tfb is a versatile gram-positive bacterium able to grow on a wide variety of aromatic compounds as carbon and energy sources. since the strain is refractory to genetic analysis, a proteomic approach was used to study the metabolic pathways involved in the catabolism of such compounds by analyzing differentially induced proteins. the most marked difference was observed when the proteome profiles of phthalate-grown cells were compared with those cultured in the presence of t ... | 2006 | 16544280 |
| biocatalytic approaches for the quantitative production of single stereoisomers from racemates. | strategies for the chemoenzymatic transformation of a racemate into a single stereoisomeric product in quantitative yield have been developed. a range of industrially relevant alpha-hydroxycarboxylic acids was deracemized in a stepwise fashion via lipase-catalysed enantioselective o-acylation, followed by mandelate racemase-catalysed racemization of the remaining non-reacted substrate enantiomer. alternatively, aliphatic alpha-hydroxycarboxylic acids were enzymatically isomerized using whole res ... | 2006 | 16545098 |
| rhodococcus and mycobacterium tuberculosis: masquerade or mixed infection. | rhodocci have a morphology similar to that of mycobacterium tuberculosis (tb), and are indistinguishable from normal diphtheroid flora. symptoms include fever, productive/non-productive cough and pleuritic chest pain. rhodococcal infections, being resistant to routine anti-tuberculosis medications, may be misdiagnosed as drug-resistant tb, thus prompting treatment for tb with rifampicin-containing regimens that promote the emergence of resistance. we present here a sputum smear afb-positive case ... | 2006 | 16562721 |
| a nucleotide mutation associated with fluoroquinolone resistance observed in gyra of in vitro obtained rhodococcus equi mutants. | in this study, the quinolone resistance-determining region (qrdr) in gyra and gyrb of in vitro fluoroquinolone-resistant rhodococcus equi mutants was sequenced. these mutants were selected from four r. equi strains on blood agar plates containing ciprofloxacin or enrofloxacin. each mutant became 8- to 64 or greater-fold resistant to fluoroquinolones compared with their parent strains. from the results of sequence analysis of qrdr in gyra and gyrb, a nucleotide mutation of codon gac for ggc in gy ... | 2006 | 16563665 |
| [the properties of hydrocarbon-oxidizing bacteria isolated from the oilfields of tatarstan, western siberia, and vietnam]. | eleven strains of hydrocarbon-oxidizing bacteria, isolated from oilfields, representing the genera rhodococcus, gordonia, dietzia, and pseudomonas, were characterized as mesophiles and neutrophiles. rhodococci were halotolerant microorganisms growing in a media containing up to 15% nacl. all the strains oxidized n-alkanes of crude oil. an influence of the cultivation temperatures (28 or 45 degrees c) and organic supplements on the degradation of c12-c30 n-alkanes in oxidized oil by two bacterial ... | 2006 | 16579448 |
| pet ownership in immunocompromised children--a review of the literature and survey of existing guidelines. | pet ownership has been associated with both emotional and physical health benefits. however, owning pets may also pose health risks to immunocompromised patients through zoonotic transmission of disease. our initial impression was that there is a lack of any evidence base in information given by health care professionals regarding these risks. we therefore aimed to produce evidence-based guidelines addressing this issue. a pubmed search was undertaken and a variety of literature on zoonoses revi ... | 2006 | 16581294 |
| polychlorinated biphenyl (pcb)-degrading bacteria associated with trees in a pcb-contaminated site. | the abundance, identities, and degradation abilities of indigenous polychlorinated biphenyl (pcb)-degrading bacteria associated with five species of mature trees growing naturally in a contaminated site were investigated to identify plants that enhance the microbial pcb degradation potential in soil. culturable pcb degraders were associated with every plant species examined in both the rhizosphere and root zone, which was defined as the bulk soil in which the plant was rooted. significantly high ... | 2006 | 16597927 |
| discovery of natural atypical nonhemolytic listeria seeligeri isolates. | we found seven listeria isolates, initially identified as isolates with the xyl(+) rha(-) biotype of listeria welshimeri by phenotypic tests, which exhibited discrepant genotypic properties in a well-validated listeria species identification oligonucleotide microarray. the microarray gives results of these seven isolates being atypical hly-negative l. seeligeri isolates, not l. welshimeri isolates. the aberrant l. seeligeri isolates were d-xylose fermentation positive, l-rhamnose fermentation ne ... | 2006 | 16597942 |
| degradation of aroclor 1242 dechlorination products in sediments by burkholderia xenovorans lb400(ohb) and rhodococcus sp. strain rha1(fcb). | burkholderia xenovorans strain lb400, which possesses the biphenyl pathway, was engineered to contain the oxygenolytic ortho dehalogenation (ohb) operon, allowing it to grow on 2-chlorobenzoate and to completely mineralize 2-chlorobiphenyl. a two-stage anaerobic/aerobic biotreatment process for aroclor 1242-contaminated sediment was simulated, and the degradation activities and genetic stabilities of lb400(ohb) and the previously constructed strain rha1(fcb), capable of growth on 4-chlorobenzoat ... | 2006 | 16597946 |
| molecular typing of vapa-positive rhodococcus equi isolates from jeju native horses, korea. | we recently demonstrated the presence of virulence-associated protein antigen (vapa)-positive rhodococcus equi in jeju island, korea. these bacteria contained one of two distinct plasmid types, a 90-kb type ii plasmid, which has been found in isolates from the native kiso horses of japan, and a new variant, a 90-kb type v plasmid. however, the genotypic characters of the vapa-positive r. equi from jeju native horses and their environments are poorly understood. ninety-eight isolates from soil sa ... | 2006 | 16598168 |
| cloning, expression, and characterization of a self-sufficient cytochrome p450 monooxygenase from rhodococcus ruber dsm 44319. | a new member of class iv of cytochrome p450 monooxygenases was identified in rhodococcus ruber strain dsm 44319. as the genome of r. ruber has not been sequenced, a p450-like gene fragment was amplified using degenerated primers. the flanking regions of the p450-like dna fragment were identified by directional genome walking using polymerase chain reaction. the primary protein structure suggests a natural self-sufficient fusion protein consisting of ferredoxin, flavin-containing reductase, and p ... | 2006 | 16607529 |
| plasmid profiles of virulent rhodococcus equi isolates from soil environment on horse-breeding farms in hungary. | the plasmid profiles of virulent rhodococcus equi strains isolated on three horse-breeding farms located in different parts of hungary were investigated. from 49 soil samples collected on the three farms, 490 r. equi isolates (10 from each sample) were obtained and tested for the presence of 15- to 17-kda antigens (vapa) by immunoblotting and pcr. ninety-eight vapa-positive isolates were detected from 30 of the 49 culture-positive samples with a prevalence ranging from 13.1% to 23.2%. of the 98 ... | 2006 | 16613022 |
| foals are interferon gamma-deficient at birth. | the increased vulnerability of foals to specific pathogens such as rhodococcus equi is believed to reflect an innate immunodeficiency, the nature of which remains poorly understood. previous studies have demonstrated that neonates of many species fail to mount potent th1 responses. the current research investigates the ability of circulating and pulmonary lymphocytes of developing foals to produce interferon gamma (ifngamma). peripheral blood mononuclear cells (pbmc) were prepared from up to 10 ... | 2006 | 16621024 |
| molecular analysis of microbial communities identified in different developmental stages of ixodes scapularis ticks from westchester and dutchess counties, new york. | ixodes scapularis ticks play an important role in the transmission of a wide variety of pathogens between various mammalian species, including humans. pathogens transmitted by ticks include borrelia, anaplasma and babesia. although ticks may harbour both pathogenic and non-pathogenic microflora, little is known about how the diversity of the microflora within ticks may influence the transmission of pathogens. to begin addressing this question, we examined the composition of bacterial communities ... | 2006 | 16623735 |
| methods for the preparation of a biodesulfurization biocatalyst using rhodococcus sp. | several methods to prepare a biodesulfurization (bds) biocatalyst were investigated in this study using a strain of rhodococcus sp. 1awq. this bacterium could selectively remove sulfur from dibenzothiophene (dbt) via the "4s" pathway. dbt, dimethylsulfoxide (dmso), sodium sulphate and mixed sulfur sources were used to study their influence on cell density, desulfurization activity, desulfurization ability, and the cost of biocatalyst production. in contrast to that observed from bacteria culture ... | 2006 | 16624377 |
| rhodococcus equi pneumonia in a foal. | 2006 | 16627120 | |
| rhodococcus kroppenstedtii sp. nov., a novel actinobacterium isolated from a cold desert of the himalayas, india. | the taxonomic position of an actinomycete, strain k07-23t, isolated from a cold desert of the himalayas, india, was established by a polyphasic approach. the strain exhibited phenotypic characters that were typical of the genus rhodococcus. 16s rrna gene sequence (1467 bases) comparisons confirmed that strain k07-23t belongs to the genus rhodococcus. 16s rrna sequence similarity studies showed that the isolate is very closely related to nocardia corynebacterioides dsm 20151t (98.6 %), which has ... | 2006 | 16627641 |
| clinical evaluation of a peptide-elisa based upon n-terminal b-cell epitope of the vapa protein for diagnosis of rhodococcus equi pneumonia in foals. | a total of 227 field samples from naturally exposed foals aged between 3 weeks and 6 months were used in an evaluation of a peptide-based enzyme-linked immunosorbent assay (elisa) for diagnosis of rhodococcus equi infection. a biotinylated peptide derived from the virulence-associated protein a (vapa) of r. equi, a horse pathogen, was synthesized and designated as pn11-14. the peptide corresponds to the n-terminal b-cell epitope tslnlqkdepngrasdtagq of the vapa protein. based upon a serum immuno ... | 2006 | 16629724 |
| evidence for the role of the monb genes in polyether ring formation during monensin biosynthesis. | ionophoric polyethers are produced by the exquisitely stereoselective oxidative cyclization of a linear polyketide, probably via a triepoxide intermediate. we report here that deletion of either or both of the monbi and monbii genes from the monensin biosynthetic gene cluster gave strains that produced, in place of monensins a and b, a mixture of c-3-demethylmonensins and a number of minor components, including c-9-epi-monensin a. all the minor components were efficiently converted into monensin ... | 2006 | 16632258 |
| mutational study on alphagln90 of fe-type nitrile hydratase from rhodococcus sp. n771. | nitrile hydratase (nhase) from rhodococcus sp. n771 is a non-heme iron enzyme having post-translationally modified cysteine ligands, alphacys112-so2h and alphacys114-soh. we replaced alphagln90, which is conserved in all known nhases and involved in the hydrogen-bond network around the catalytic center, with glutamic acid or asparagine. the kcat of alphaq90e and alphaq90n mutants decreased to 24% and 5% that of wild type respectively, but the effect of mutations on km was not very significant. i ... | 2006 | 16636455 |
| microbial desulfurization of gasoline by free whole-cells of rhodococcus erythropolis xp. | rhodococcus erythropolis xp could grow well with condensed thiophenes, mono-thiophenic compounds and mercaptans present in gasoline. rhodococcus erythropolis xp was also capable of efficiently degrading the condensed thiophenes in resting cell as well as biphasic reactions in which n-octane served as a model oil phase. free whole cells of r. erythropolis xp were adopted to desulfurize fluid catalytic cracking (fcc) and straight-run (sr) gasoline oils. about 30% of the sulfur content of fcc gasol ... | 2006 | 16640586 |
| structural analysis of an extracellular polysaccharide produced by rhodococcus rhodochrous strain s-2. | a possibility has been suggested of applying the eps produced by rhodococcus rhodochrous strain s-2 (s-2 eps) to the bioremediation of oil-contaminated environments, because its addition, together with minerals, to oil-contaminated seawater resulted in emulsification of the oil, increased the degradation of polyaromatic hydrocarbons (pah) of the oil, and led to the dominance of pah-degrading marine bacteria. to understand the underlying principles of these phenomena, we determined the chemical s ... | 2006 | 16676419 |
| comparison of concentrations of rhodococcus equi and virulent r. equi in air of stables and paddocks on horse breeding farms in a temperate climate. | rhodococcoccus equi is a significant cause of bronchopneumonia in foals worldwide. infection of the lungs is believed to result from inhalation of virulent r. equi in dust from contaminated environments. a measure of infectious risk in an environment is the level of airborne contamination. | 2006 | 16706283 |
| the impact of bioaugmentation on metal cyanide degradation and soil bacteria community structure. | metal cyanides are significant contaminants of many soils found at the site of former industrial activity. in this study we isolated bacteria capable of degrading ferric ferrocyanide and k2ni(cn)4. one of these bacteria a rhodococcus spp. was subsequently used to bioaugment a minimal medium broth, spiked with k2ni(cn)4, containing 1 g of either an uncontaminated topsoil or a former coke works site soil. degradation of the k2ni(cn)4 was observed in both soils, however, bioaugmentation did not sig ... | 2006 | 16715400 |
| [rhodococcus equi]. | 2006 | 16721450 | |
| enzymatic c-4 deacetylation of 10-deacetylbaccatin iii. | second-generation paclitaxel analogues that require replacement of the c-4 acetate by other substituents are in development. an enzyme able to specifically remove the c-4 acetate from paclitaxel could simplify preparation of the analogues. several strains were isolated from soil samples that contain enzyme activities able to 4-deacetylate 10-dab (10-deacetylbaccatin iii). selection was made using plates containing 10-dab as the sole carbon source and screening colonies for deacetylation of 10-da ... | 2006 | 16722823 |
| bioremediation of halogenated compounds: comparison of dehalogenating bacteria and improvement of catalyst stability. | five bacterial strains were compared for halogenated compounds conversion in aqueous media. depending on the strain, the optimal temperature for dehalogenase activity of resting cells varied from 30 to 45 degrees c, while optimal ph raised from 8.4 to 9.0. the most effective dehalogenase activity for 1-chlorobutane conversion was detected with rhodococcus erythropolis ncimb13064 and escherichia coli bl21 (de3) (dhaa). the presence of 2-chlorobutane or propanal in the aqueous media could inhibit ... | 2006 | 16723151 |
| characterization of rhodococcus-e. coli shuttle vector pnc9501 constructed from the cryptic plasmid of a propene-degrading bacterium. | rhodococcus-e. coli shuttle vector pnc9501 was constructed using circular cryptic plasmid pnc903 from propene-degrading rhodococcus ruber p-ii-123-1. sequence analysis of pnc903 revealed two open-reading frames encoding the replication proteins reps a and b. in the amino acid sequence of the putative rep b, a helix-turn-helix motif, which is responsible for the binding of dna, was found. sequencing of the upstream region of the putative rep a and incompatibility tests revealed that pnc903 is a m ... | 2006 | 16732453 |
| biocatalytic deuterium- and hydrogen-transfer using over-expressed adh-'a': enhanced stereoselectivity and 2h-labeled chiral alcohols. | employing the over-expressed highly organic solvent tolerant alcohol dehydrogenase adh-'a' from rhodococcus ruber dsm 44541, versatile building blocks, which were not accessible by the wild type catalyst, were obtained in > 99% e.e.; furthermore, employing d8-2-propanol as deuterium source, stereoselective biocatalytic deuterium transfer was made feasible to furnish enantiopure deuterium labeled sec-alcohols on a preparative scale employing a single enzyme. | 2006 | 16733594 |
| genetic and biochemical characterization of the dioxygenase involved in lateral dioxygenation of dibenzofuran from rhodococcus opacus strain sao101. | rhodococcus opacus strain sao101 was shown to degrade on various polycyclic aromatic hydrocarbons such as naphthalene, dibenzofuran (df), and dibenzo-p-dioxin (dd). one of the unique traits of the strain sao101 is its ability to oxidize df compounds by lateral dioxygenation. to clone the lateral dioxygenase gene involved in compound degradation in strain sao101, we identified a cosmid clone that oxidizes aromatic compounds by using sao101 genomic dna. sequencing analysis revealed that isolated c ... | 2006 | 16736088 |
| [co-expression of rhodococcus sp. ds-3 dszabc and dszd gene with incompatible plasmids in escherichia coli]. | biological dusulfurizaion of petroleum feedstocks and products may offer an attractive alternative to reduce sulfur oxide emissions that cause serious environmental pollution. dibenzothiophene (dbt), a model of organic sulfur compound in petroleum, can be microbially desulfurized without degradation of the organic structure by 4s pathway. three desulfurization enzymes (dsza, dszb and dszc) and flavin reductase (dszd) are involved in sulfur-specific dbt desulfurization. dsza and dszc are fmnh2-de ... | 2006 | 16736591 |
| proteome changes after metabolic engineering to enhance aerobic mineralization of cis-1,2-dichloroethylene. | metabolically engineered escherichia coli has previously been used to degrade cis-1,2-dichloroethylene (cis-dce). the strains express the six genes of an evolved toluene ortho-monooxygenase from burkholderia cepacia g4 (tom-green, which formed a reactive epoxide) with either (1) gamma-glutamylcysteine synthetase (gshi, which forms glutathione) and the glutathione s-transferase isoilr1 from rhodococcus ad45 (which adds glutathione to the reactive cis-dce epoxide) or (2) with an evolved epoxide hy ... | 2006 | 16739990 |
| internally controlled real-time pcr method for quantitative species-specific detection and vapa genotyping of rhodococcus equi. | we developed a novel quantitative real-time pcr (q-pcr) method for the soil actinomycete rhodococcus equi, an important horse pathogen and emerging human pathogen. species-specific quantification was achieved by targeting the chromosomal monocopy gene choe, universally conserved in r. equi. the choe q-pcr included an internal amplification control (iac) for identification of false negatives. a second q-pcr targeted the virulence plasmid gene vapa, carried by most horse isolates but infrequently ... | 2006 | 16751540 |
| lariatins, antimycobacterial peptides produced by rhodococcus sp. k01-b0171, have a lasso structure. | two antimycobacterial agents, lariatins a and b, were isolated from the culture broth of rhodococcus sp. k01-b0171. their structures were elucidated by spectral analysis and advanced protein chemical methods to be unique cyclic peptides, which consist of 18 and 20 l-amino acid residues with an internal linkage between the gamma-carboxyl group of glu8 and the alpha-amino group of gly1. the three-dimensional structure of lariatin a deduced from nmr data by dynamical simulated annealing method indi ... | 2006 | 16756302 |
| [the prospects of using bacteria of the genus rhodococcus and microbial surfactants for the degradation of oil pollutants]. | the possibility of accelerating oil degradation by an enrichment culture of oil-oxidizing microorganisms in the presence of bacteria of the genus rhodococcus and microbial surfactants was studied. it was shown that the degree of consumption of crude oil (2% v/v) after 192 h of enrichment culture growth reached 84%. inoculation of the active hydrocarbon-oxidizing strain rhodococcus erythropolis ek-1 and exogenous surfactants produced by pseudomonas sp. ps-27 increased this degree to 90% and 93-94 ... | 2006 | 16761570 |
| bioremediation of environmental endocrine disruptor di-n-butyl phthalate ester by rhodococcus ruber. | in this study dbp-degradation strain cq0301 was isolated from rubbish landfill soil. according to the biophysical, biochemical characteristics and analysis of 16s rrna, the strain was identified as rhodococcus ruber. three new protein bands could be fractioned after dbp-inducing, which were suspected to participate the process of dbp-degrading. catechol was suspected to be an intermediate product of dbp and cleaving the benzene ring was catalyzed by catechol 1,2-dioxygenase, because a highly act ... | 2006 | 16762396 |
| rhodococcus equi--an emerging human pathogen in immunocompromized hosts: a report of four cases from malaysia. | rhodococcus equi, a recognized pathogen in horses, is emerging as a human opportunistic pathogen, especially in immunocompromized hosts. we describe four immunocompromized patients who had serious r. equi infections with an overall mortality of 75%. the natural habitat of r. equi is soil, particularly soil contaminated with animal manure. necrotizing pneumonia is the commonest form of infection but extrapulmonary infections, such as wound infections and subcutaneous abscess, have also been descr ... | 2006 | 16771229 |
| isolation and characterization of a carbendazim-degrading rhodococcus sp. djl-6. | bacterium djl-6, capable of degrading carbendazim, was isolated by continuous enrichment culture originating from carbendazim-treated soil. the isolate was identified as rhodococcus sp. according to its phenotypic features, physiologic and biochemical characteristics, and phylogenetic analysis. the strain could use carbendazim as sole carbon or nitrogen source. it showed a high average degradation rate of 55.56 mg . l(-1) . d(-1) in m9 medium amended with carbendazim. high-pressure liquid chroma ... | 2006 | 16775791 |
| interaction of metabolites with r. rhodochrous during the biodegradation of di-ester plasticizers. | the commonly used plasticizers di-ethylhexyl phthalate (dehp) and di-ethylhexyl adipate (deha) are known to partially degrade in the presence of soil microorganisms, such as rhodococcus rhodochrous, releasing persistent and toxic metabolites. the metabolites adipic acid and 2-ethylhexanol were both shown to inhibit growth of the degrading microbe. 2-ethylhexanol enhanced the activity of ethanol dehydrogenase - an enzyme involved in its metabolism - but the activity of this enzyme was inhibited b ... | 2006 | 16777176 |
| resuscitation-promoting factors: distribution among actinobacteria, synthesis during life-cycle and biological activity. | we have identified new members of the resuscitation-promoting factor (rpf) protein family in numerous as yet unsequenced actinobacteria suggesting that rpfs are common in this bacterial group. formation and final delivery of the rpf proteins varied from strain to strain and were dependent on growth conditions. rpfs were found to act complementary to resuscitate other actinobacteria and might therefore be useful tools for the isolation of new actinobacterial species, especially from soil samples ... | 2006 | 16779632 |
| immunogenicity of synthetic rhodococcus equi virulence-associated protein peptides in neonate foals. | rhodococcus equi infection is considered the most common cause of pneumonia in foals less than 6 months of age. immunization of foals and/or mares may become a procedure of choice for prevention. the present work documents the antibody response of neonate foals to r. equi virulence-associated protein (vap) vaccine candidate peptides. a mixture of 4 r. equi (atcc 33701) vap peptides was selected based on their hydrophilicity and recognition by naturally acquired igg antibodies from 13 adult horse ... | 2006 | 16782401 |
| a regulatory effect of the balance between tnf-alpha and il-6 in the granulomatous and inflammatory response to rhodococcus aurantiacus infection in mice. | after i.v. inoculation with rhodococcus aurantiacus, wild-type (wt) mice develop nonnecrotic, epithelioid granulomas. because a high level of tnf-alpha is observed during the initial phase postinfection, we examined the extent to which tnf-alpha contributes to granulomatous inflammation using tnf-alpha gene-deficient (tnf-alpha(-/-)) mice. despite a lack of r. aurantiacus proliferation, tnf-alpha(-/-) mice displayed high mortality rates within 5 days postinfection, as well as a high level of il- ... | 2006 | 16785562 |
| caver: a new tool to explore routes from protein clefts, pockets and cavities. | the main aim of this study was to develop and implement an algorithm for the rapid, accurate and automated identification of paths leading from buried protein clefts, pockets and cavities in dynamic and static protein structures to the outside solvent. | 2006 | 16792811 |
| crystal structure of 3-chlorocatechol 1,2-dioxygenase key enzyme of a new modified ortho-pathway from the gram-positive rhodococcus opacus 1cp grown on 2-chlorophenol. | the crystal structure of the 3-chlorocatechol 1,2-dioxygenase from the gram-positive bacterium rhodococcus opacus (erythropolis) 1cp, a fe(iii) ion-containing enzyme specialized in the aerobic biodegradation of 3-chloro- and methyl-substituted catechols, has been solved by molecular replacement techniques using the coordinates of 4-chlorocatechol 1,2-dioxygenase from the same organism (pdb code 1s9a) as a starting model and refined at 1.9 a resolution (r(free) 21.9%; r-factor 17.4%). the analysi ... | 2006 | 16793061 |
| overcoming the thermodynamic limitation in asymmetric hydrogen transfer reactions catalyzed by whole cells. | whole lyophilized cells of an escherichia coli overexpressing the alcohol dehydrogenase (adh-'a') from rhodococcus ruber dsm 44541 were used for the asymmetric reduction of ketones to secondary alcohols. the recycling of the required nicotinamide cofactor (nadh) was achieved in a coupled-substrate process. in the course of the reaction the ketone is reduced to the alcohol and the hydrogen donor 2-propanol is oxidized to acetone by one enzyme. this leads to a thermodynamic equilibrium between all ... | 2006 | 16804944 |
| desulfurization of dibenzothiophene by bacillus subtilis recombinants carrying dszabc and dszd genes. | the desulfurization (dsz) genes from rhodococcus erythropolis ds-3 were successfully integrated into the chromosomes of bacillus subtilis atcc 21332 and uv1 using an integration vector pdgsdn, yielding two recombinant strains, b. subtilis m29 and m28 in which the integrated dsz genes were expressed efficiently under the promoter, pspac. the dibenzothiophene (dbt) desulfurization efficiency of m29 was 16.2 mg dbt l(-1) h(-1) at 36 h, significantly higher than that of r. erythropolis ds-3 and b. s ... | 2006 | 16810451 |
| biodesulfurization in biphasic systems containing organic solvents. | biphasic systems can overcome the problem of low productivity in conventional media and have been exploited for biocatalysis. solvent-tolerant microorganisms are useful in biotransformation with whole cells in biphasic reactions. a solvent-tolerant desulfurizing bacterium, pseudomonas putida a4, was constructed by introducing the biodesulfurizing gene cluster dszabcd, which was from rhodococcus erythropolis xp, into the solvent-tolerant strain p. putida idaho. biphasic reactions were performed t ... | 2006 | 16820450 |
| etiological misidentification by routine biochemical tests of bacteremia caused by gordonia terrae infection in the course of an episode of acute cholecystitis. | gordonia terrae has been reported to be a rare cause of bacteremia. we report the first case of bacteremia associated with acute cholecystitis. commercial biochemical testing was not able to identify the strain at the genus level, classifying it instead as rhodococcus sp. definitive identification was obtained by sequencing of the 16s rrna gene. | 2006 | 16825404 |
| biodegradation by members of the genus rhodococcus: biochemistry, physiology, and genetic adaptation. | 2006 | 16829254 | |
| rhodococcus sp. strain tm1 plays a synergistic role in the degradation of piperidine by mycobacterium sp. strain tho100. | mycobacterium sp. strain tho100 and rhodococcus sp. strain tm1 were isolated from a morpholine-containing enrichment culture of activated sewage sludge. strain tho100, but not strain tm1, was able to degrade alicyclic amines such as morpholine, piperidine, and pyrrolidine. the mixed strains tho100 and tm1 showed a better growth on piperidine as the substrate than the pure strain tho100 because strain tm1 was able to reduce the level of glutaraldehyde (ga) produced during piperidine degradation. ... | 2006 | 16832627 |
| [pneumonia caused rhodococcus equi in a immunocompromised patient treated with corticosteroids]. | 2006 | 16834958 | |
| dramatic enhancement of enantioselectivity of biotransformations of beta-hydroxy nitriles using a simple o-benzyl protection/docking group. | [structure: see text] catalyzed by the rhodococcus erythropolis aj270 whole cell catalyst, the o-benzylated beta-hydroxy alkanenitriles underwent remarkably high enantioselective biotransformations, whereas the biotransformations of free beta-hydroxy alkanenitriles gave very low enantioselectivity. the easy manipulations of o-protection and o-deprotection, excellent chemical and enantiomeric yields of biotransformations, along with the scalability render this enzymatic transformation attractive ... | 2006 | 16836373 |
| replication and inheritance of nocardia plasmid pc1. | nocardia sp. c-14-1, isolated from acrylic fiber wastewater, can degrade long-chain alkanes and succinonitrile efficiently. here we report the characterization of an indigenous plasmid pc1. the overall nucleotide sequence of pc1 consisted of 5841 bp. the five orfs, encoding a dna recombinase, replication protein (rep(pc1)) and three proteins of unknown function, were predicted on pc1. the rep(pc1) displayed its homology with the rep of rhodococcus large plasmid p33701, suggesting a theta type of ... | 2006 | 16842357 |
| n-acylhomoserine lactonase producing rhodococcus spp. with different ahl-degrading activities. | n-acylhomoserine lactones (ahls) are conserved signal molecules that control diverse biological activities in quorum sensing system of gram-negative bacteria. recently, several soil bacteria were found to degrade ahls, thereby interfering with the quorum sensing system. previously, rhodococcus erythropolis w2 was reported to degrade ahls by both oxido-reductase and ahl-acylase. in the present study, two ahl-utilizing bacteria, strains ls31 and pi33, were isolated and identified as the genus rhod ... | 2006 | 16842366 |
| proteasome assembly triggers a switch required for active-site maturation. | the processing of propeptides and the maturation of 20s proteasomes require the association of beta rings from two half proteasomes. we propose an assembly-dependent activation model in which interactions between helix (h3 and h4) residues of the opposing half proteasomes are prerequisite for appropriate positioning of the s2-s3 loop; such positioning enables correct coordination of the active-site residue needed for propeptide cleavage. mutations of h3 or h4 residues that participate in the ass ... | 2006 | 16843899 |
| detection, isolation and partial characterization of an immunostimulating glycoprotein from rhodococcus fascians. | in a search for novel immunostimulating substances we detected that culture supernatants of the gram-positive phytopathogenic bacterium, rhodococcus fascians, were able to induce cytokine release (tnf(alpha)) from mouse peritoneal macrophages. monoclonal antibodies were generated against the active principle, and were employed for its isolation and partial characterization as a high molecular (mw>100 kda) glycoprotein. in addition, methods practicable for its biotechnological preparation and sev ... | 2006 | 16846838 |
| modulation of eukaryotic cell apoptosis by members of the bacterial order actinomycetales. | apoptosis, or programmed cell death, is normally responsible for the orderly elimination of aged or damaged cells, and is a necessary part of the homeostasis and development of multicellular organisms. some pathogenic bacteria can disrupt this process by triggering excess apoptosis or by preventing it when appropriate. either event can lead to disease. there has been extensive research into the modulation of host cell death by microorganisms, and several reviews have been published on the phenom ... | 2006 | 16850163 |
| vapi, a new member of the rhodococcus equi vap family. | rhodococcus equi is a facultative intracellular bacterium which can cause bronchopneumonia in foals and aids patients. in this report we show that the orf13-protein coded by the virulence associated plasmid of r. equi is clearly homologous to vape. nucleotide sequence analysis revealed frame shift mutations that shorten the sequence of the orf13-protein. a theoretical extension of the sequence of orf13 by the introduction of a single nucleotide yields a translated amino acid sequence that is hig ... | 2006 | 16871422 |
| culturable actinobacteria from the marine sponge hymeniacidon perleve: isolation and phylogenetic diversity by 16s rrna gene-rflp analysis. | a total of 106 actinobacteria associated with the marine sponge hymeniacidon perleve collected from the yellow sea, china were isolated using eight different media. the number of species and genera of actinobacteria recovered from the different media varied significantly, underlining the importance of optimizing the isolation conditions. the phylogenetic diversity of the actinobacteria isolates was assessed using 16s rrna gene amplification-restriction fragment length polymorphism (rflp) analysi ... | 2006 | 16871424 |
| the isolation of microorganisms capable of phenol degradation. | the results of a study on the composition of microflora settling the pilot biofilter bed that purifies the exhausting gases from a cable factory's coil-wire varnishing division are presented in this study. the ability of isolated bacterial strains to biodegrade phenol was also evaluated using culture media of various compositions. phenol was introduced into the medium at the following concentrations: 0.25, 0.5, 0.75 and 1 g x dm(-3). in addition, air in desiccators, where micro-organisms grew, w ... | 2006 | 16878606 |
| multiple-subunit genes of the aromatic-ring-hydroxylating dioxygenase play an active role in biphenyl and polychlorinated biphenyl degradation in rhodococcus sp. strain rha1. | a gram-positive strong polychlorinated biphenyl (pcb) degrader, rhodococcus sp. strain rha1, can degrade pcbs by cometabolism with biphenyl or ethylbenzene. in rha1, three sets of aromatic-ring-hydroxylating dioxygenase genes are induced by biphenyl. the large and small subunits of their terminal dioxygenase components are encoded by bpha1 and bpha2, etba1 and etba2, and ebda1 and ebda2, respectively, and the deduced amino acid sequences of etba1 and etba2 are identical to those of ebda1 and ebd ... | 2006 | 16885291 |
| crystal structure and desulfurization mechanism of 2'-hydroxybiphenyl-2-sulfinic acid desulfinase. | the desulfurization of dibenzothiophene in rhodococcus erythropolis is catalyzed by two monooxygenases, dsza and dszc, and a desulfinase, dszb. in the last step of this pathway, dszb hydrolyzes 2'-hydroxybiphenyl-2-sulfinic acid into 2-hydroxybiphenyl and sulfite. we report on the crystal structures of dszb and an inactive mutant of dszb in complex with substrates at resolutions of 1.8a or better. the overall fold of dszb is similar to those of periplasmic substrate-binding proteins. in the subs ... | 2006 | 16891315 |
| rhodococcus equi in the soil environment of horses in inner mongolia, china. | little is known about the distribution of rhodococcus equi in the soil environment of native horses in china. one hundred and eight soil samples were collected from native-horse farms in the hulun beier grasslands of eastern mongolia, the xilin goler grasslands of southern mongolia, and tongliao city in inner mongolia, china. the isolation rates of r. equi from soil samples from the hulun beier and xilin goler grasslands ranged from 25.9% to 30.0%. in contrast, isolation rates from soil samples ... | 2006 | 16891789 |
| nitrile hydrolysis activity of rhodococcus erythropolis ncimb 11540 whole cells. | the nitrile hydrolyzing properties of the bacterium strain rhodococcus erythropolis ncimb 11540 have been investigated. using whole cells of the microorganism, a wide variety of aromatic and aliphatic cyanide-containing substrates was successfully hydrolyzed to the corresponding amide or acid. in the case of dicyanides, selective monohydrolysis took place, which was further explored in the desymmetrization of malononitriles resulting in the corresponding cyano amides in enantiomeric excesses of ... | 2006 | 16892293 |
| variations of bacterial community structure in flooded paddy soil contaminated with herbicide quinclorac. | the denaturing gradient gel electrophoresis (dgge) method was applied to determine the relative genetic complexity of microbial communities in flooded paddy soil treated with herbicide quinclorac (3,7-dichloro-8-quinoline-carboylic acid). the results obtained showed a significant effect of quinclorac on the development of bacterial populations in soils contaminated with different concentrations of the herbicide at the early time after application. in general, however, the number of populations o ... | 2006 | 16893772 |
| rhodococcus imtechensis sp. nov., a nitrophenol-degrading actinomycete. | a gram-positive actinobacterium, strain rkj300(t), capable of utilizing p-nitrophenol and 2,4-dinitrophenol, was isolated from a pesticide-contaminated site in india. the morphological and chemotaxonomic properties of the isolate were typical of members of the genus rhodococcus. the dna g+c content was 72 mol%. strain rkj300(t) exhibited the highest level of sequence similarity with rhodococcus wratislaviensis ncimb 13082(t) (99.3 %), followed by rhodococcus opacus dsm 43205(t) (98.8 %), rhodoco ... | 2006 | 16902038 |
| biosurfactant production by rhodococcus erythropolis grown on glycerol as sole carbon source. | the production of biosurfactant by rhodococcus erythropolis during the growth on glycerol was investigated. the process was carried out at 28 degrees c in a 1.5-l bioreactor using glycerol as carbon source. the bioprocess was monitored through measurements of biosurfactant concentration and glycerol consumption. after 51 h of cultivation, 1.7 g/l of biosurfactant, surface, and interfacial tensions values (with n-hexadecane) of 43 and 15 mn/m, respectively, 67% of emulsifying index (e24), and 94% ... | 2006 | 16915696 |
| [performance of demulsification by rhodococcus sp. pr-1]. | rhodococcus sp. pr-1 with high capability of demulsification on a surfactant-stabilized kerosene-water model emulsion was isolated from sewage of dagang oil field. it could demulsify the model emulsion completely in 8 hours at 55 degrees c and had better demulsifying capability than chemical demulsifier dgf-01. the freezing-thawing and autoclaving had no effect on the process of demulsification, yet ultrasonic disposal and deal with organic solvent could inhibit its activity. a linear relationsh ... | 2006 | 16921960 |
| genetic diversity of dioxygenase genes in polycyclic aromatic hydrocarbon-degrading bacteria isolated from mangrove sediments. | to investigate the diversity of dioxygenase genes involved in polycyclic aromatic hydrocarbon (pah)-degradation, a total of 32 bacterial strains were isolated from surface mangrove sediments, from the genera mycobacterium, sphingomonas, terrabacter, sphingopyxis, sphingobium and rhodococcus. two sets of pcr primers were constructed to detect the nida-like and nahac-like sequences of the alpha subunit of the pah ring-hydroxylating dioxygenase. pcr amplified the dna fragments from all gram-positiv ... | 2006 | 16923069 |
| protocol for mutagenesis of alkene monooxygenase and screening for modified enantiocomposition of the epoxypropane product. | alkene monooxygenase (amo) from rhodococcus rhodochrous b-276 is a 3-component enzyme system encoded by the 4-gene operon amoabcd, which catalyzes the stereoselective epoxidation of aliphatic alkenes yielding primarily the r enantiomer. with propene as the substrate, wild-type amo yields r-epoxypropane with an enantiomeric excess (e.e.) of 83%. the presumed site of alkene oxidation is a dinuclear iron center situated within the large subunit of the epoxygenase component, amoc. substantial proble ... | 2006 | 16928986 |
| [long term survival after rhodococcus equi pneumonia in a patient with human immunodeficiency virus infection in the era of highly active antiretroviral therapy: case report and review]. | before highly active antiretroviral therapy (haart) has become available, antibiotic treatment was usually unable to eradicate rhodococcus (r.) equi infection in hiv-infected patients, although some clinical improvement could be observed in most cases. there are limited data on the outcome of treatment of r. equi pneumonia in the haart era. we report on a 52-year-old hiv-infected man who presented in poor general condition with an extensive lung cavitation lesion caused by r. equi. the patient r ... | 2006 | 16933840 |
| simultaneous biodetoxification of s, n, and o pollutants by engineering of a carbazole-degrading gene cassette in a recombinant biocatalyst. | the gene cassette encoding enzymes responsible for degrading carbazole to anthranilic acid was introduced into a dibenzothiophene degrader. the resultant strain, rhodococcus erythropolis xpdn, could simultaneously transform the model pollutants dibenzothiophene, carbazole, and dibenzofuran to nontoxic metabolites and may have an application potential for bioremediation. | 2006 | 16936043 |
| characterization of nocardia plasmid pxt107. | nocardia, rhodococcus and streptomyces, all members of the actinomycetes family, are gram-positive eubacteria with high g+c content and able to form mycelium. we report here a newly identified plasmid pxt107 of nocardia sp. 107, one of the smallest circular plasmids found in nocardia. the complete nucleotide sequence of pxt107 consisted of 4335 bp with 65% g+c content, and encoded one replication extragenic palindromic (rep) and six hypothetical proteins. the rep, double-strand origin and single ... | 2006 | 16953300 |