Publications

improvement of catalytic properties of lipase from arthrobacter sp. by encapsulation in hydrophobic sol-gel materials.	in this work, lipase from arthrobacter sp. was immobilized by sol-gel encapsulation to improve its catalytic properties. various silanizing agents including vinyl-trimethoxy silane, octyl-trimethoxy silane, gamma-(methacryloxypropyl)-trimethoxy silane (maptms) and tetraethoxysilane (teos) were chosen as the precursors. among them, maptms was for the first time utilized to encapsulate lipases, and the prepared enzyme by copolymerization of maptms and teos exhibited the highest activity in both th ...	2009	19428242
ecotoxicological characterisation of 12 incineration ashes using 6 laboratory tests.	in the european waste list (2000/532/ec as amended) the ash of municipal waste incineration is defined as a so called mirror entry. this waste can be classified as hazardous or non-hazardous depending on the content of hazardous substances and other risk properties. for the assignment of waste in mirror entries, 14 criteria are defined. one of the criteria is h14 "ecotoxic". in the presented study, the ecotoxicological potential of 12 ashes from different incineration plants has been assessed us ...	2009	19442505
uptake of l-nicotine and of 6-hydroxy-l-nicotine by arthrobacter nicotinovorans and by escherichia coli is mediated by facilitated diffusion and not by passive diffusion or active transport.	the mechanism by which l-nicotine is taken up by bacteria that are able to grow on it is unknown. nicotine degradation by arthrobacter nicotinovorans, a gram-positive soil bacterium, is linked to the presence of the catabolic megaplasmid pao1. l-[(14)c]nicotine uptake assays with a. nicotinovorans showed transport of nicotine across the cell membrane to be energy-independent and saturable with a k(m) of 6.2+/-0.1 microm and a v(max) of 0.70+/-0.08 micromol min(-1) (mg protein)(-1). this is in ac ...	2009	19443550
membrane bioreactor treatment of a simulated metalworking fluid wastewater containing ethylenediaminetetraacetic acid and dicyclohexylamine.	membrane bioreactors (mbrs) have been installed at automotive plants to treat metalworking fluid (mwf) wastewaters, which are known to contain toxic and/or recalcitrant organic compounds. a laboratory study was conducted to evaluate treatment of a simulated wastewater prepared from a semisynthetic mwf, which contains two such compounds, dicyclohexylamine (dcha) and ethylenediaminetetraacetic acid (edta). primary findings were as follows: during stable operating periods, almost all chemical oxyge ...	2009	19445324
[effect of elevation of 1-hydroxy-2-naphthoic acid concentration on the lag phase of arthrobacter sp. k3: kinetic analysis].		2009	19449734
viability and potential for immigration of airborne bacteria from africa that reach high mountain lakes in europe.	we have analysed the diversity of the bacteria, which grow after addition of concentrated airborne particles and desert dust in different microcosms combinations with water samples from oligotrophic alpine lakes. we used, on the one hand, airborne bacteria transported by an african dust plume and collected in a high mountain area in the central pyrenees (spain). on the other hand, we collected desert dust in mauritania (c. 3000 km distance, and a few days estimated airborne journey), a known sou ...	2009	19453609
[effect of a novel fibrinolytic enzyme fa-i on thrombosis and thrombolysis].	to study the effect of fa-i, a novel fibrinolytic enzyme isolated from the metabolite of arthrobacter sp. dr-536, on thrombosis and thrombolysis in vivo.	2009	19462909
molecular design of a nylon-6 byproduct-degrading enzyme from a carboxylesterase with a beta-lactamase fold.	a carboxylesterase with a beta-lactamase fold from arthrobacter possesses a low level of hydrolytic activity (0.023 mumol.min(-1).mg(-1)) when acting on a 6-aminohexanoate linear dimer byproduct of the nylon-6 industry (ald). g181d/h266n/d370y triple mutations in the parental esterase increased the ald-hydrolytic activity 160-fold. kinetic studies showed that the triple mutant possesses higher affinity for the substrate ald (k(m) = 2.0 mm) than the wild-type ald hydrolase from arthrobacter (k(m) ...	2009	19476493
microbial ecology of gorgonzola rinds and occurrence of different biotypes of listeria monocytogenes.	in this study we investigated the microbiota of gorgonzola rinds and maturing shelf swabs collected in 5 different maturing cellars in the northwest part of italy, in association with the detection and characterization of listeria monocytogenes. culture-dependent and -independent methods were performed in order to profile the main microbial populations present on the rinds and in the maturing shelves and species-specific pcr and pulsed field gel electrophoresis (pfge) were used to identify and t ...	2009	19477544
microbial responses to mustard gas dumped in the baltic sea.	microbiological studies were carried out on chemical weapon dump sites in the baltic sea. the effect of mustard gas hydrolysis products (mghps) on marine microbiota and the ability of microorganisms to degrade mghps were studied. many stations at the dump sites demonstrated reduced microbial diversity, and increased growth of species able to use mustard gas hydrolysis products as sole source of carbon. significant amounts of mghp-degrading bacteria were revealed in the near-bottom water. the mgh ...	2009	19481794
identification of a novel self-sufficient styrene monooxygenase from rhodococcus opacus 1cp.	sequence analysis of a 9-kb genomic fragment of the actinobacterium rhodococcus opacus 1cp led to identification of an open reading frame encoding a novel fusion protein, stya2b, with a putative function in styrene metabolism via styrene oxide and phenylacetic acid. gene cluster analysis indicated that the highly related fusion proteins of nocardia farcinica ifm10152 and arthrobacter aurescens tc1 are involved in a similar physiological process. whereas 413 amino acids of the n terminus of stya2 ...	2009	19482928
bacterial diversity of soil samples from the western himalayas, india.	high-altitude cold habitats of the himalayas are little explored with respect to bacterial diversity. diverse bacterial species and phylotypes obtained by culture-dependent and culture-independent approaches are reported here. phylogenetic analysis and modulation of bacterial diversity with altitude and available organic carbon content are also described. psychrophilic and psychrotolerant bacteria dominated the himalayan habitats, accounting for 60% of the cultivated strains. isolates produced o ...	2009	19483785
batch and continuous biosorption of cu(2+) by immobilized biomass of arthrobacter sp.	the ability of free and polysulphone immobilized biomass of arthrobacter sp. to remove cu(2+) ions from aqueous solution was studied in batch and continuous systems. the langmuir and freundlich isotherm models were applied to the data. the langmuir isotherm model was found to fit the sorption data indicating that sorption was monolayer and uptake capacity (q(o)) was 175.87 and 158.7mg/g for free and immobilized biomass respectively at ph 5.0 and 30 degrees c temperature, which was also confirmed ...	2009	19487070
glycinebetaine-induced water-stress tolerance in coda-expressing transgenic indica rice is associated with up-regulation of several stress responsive genes.	rice (oryza sativa l.), a non-accumulator of glycinebetaine (gb), is highly susceptible to abiotic stress. transgenic rice with chloroplast-targeted choline oxidase encoded by the coda gene from arthrobacter globiformis has been evaluated for inheritance of transgene up to r5 generation and water-stress tolerance. during seedling, vegetative and reproductive stages, transgenic plants could maintain higher activity of photosystem ii and they show better physiological performance, for example, enh ...	2009	19490479
optimization of physical parameters for lipase production from arthrobacter sp. bgcc#490.	the physical parameters for the production of thermostable, alkaline lipase from arthrobacter sp. bgcc# 490 were optimized using response surface methodology (rsm), employing face centered central composite design (fcccd). the design was employed by selecting ph, temperature and incubation period as the model factors and to achieve maximum yield, interaction of these factors was studied by rsm. a second-order quadratic model and response surface method showed that the optimum conditions for lipa ...	2009	19517996
two alternative modes for optimizing nylon-6 byproduct hydrolytic activity from a carboxylesterase with a beta-lactamase fold: x-ray crystallographic analysis of directly evolved 6-aminohexanoate-dimer hydrolase.	promiscuous 6-aminohexanoate-linear dimer (ald)-hydrolytic activity originally obtained in a carboxylesterase with a beta-lactamase fold was enhanced about 80-fold by directed evolution using error-prone pcr and dna shuffling. kinetic studies of the mutant enzyme (hyb-s4m94) demonstrated that the enzyme had acquired an increased affinity (k(m) = 15 mm) and turnover (k(cat) = 3.1 s(-1)) for ald, and that a catalytic center suitable for nylon-6 byproduct hydrolysis had been generated. construction ...	2009	19521995
synthesis of 9-beta-d-arabinofuranosylguanine by combined use of two whole cell biocatalysts.	unlike the preparation of other purine nucleosides, transglycosylation from a pyrimidine nucleoside and guanine is difficult because of the low solubility of this base. thus, another strategy, based on the coupled action of two whole cell biocatalyzed reactions, transglycosylation and deamination, was used. enterobacter gergoviae and arthrobacter oxydans were employed to synthesize 9-beta-d-arabinofuranosylguanine (arag), an efficient anti leukemic drug.	2009	19523826
isolation and characterization of arthrobacter sp. hy2 capable of degrading a high concentration of p-nitrophenol.	a soil bacterium strain, capable of using p-nitrophenol (pnp) as its sole source of carbon and energy, was isolated by enrichment on minimal salt medium (msm). on the basis of a phylogenetic analysis of 16s rrna gene sequences the bacterium is a species of arthrobacter, closely related to arthrobacter ureafaciens dsm 20126. this strain has an unusually high substrate tolerance for pnp degradation in msm. greatest degradation of pnp was observed at 30 degrees c and under slightly alkaline ph (ph ...	2009	19540107
azolla-anabaena's behaviour in urban wastewater and artificial media--influence of combined nitrogen.	the results of using the nitrogen fixing symbiotic system azolla-anabaena to improve the quality of treated urban wastewater, particularly on what concerns phosphorus removal efficiencies (40-65%), obtained in continuous assays performed during the past few years and presented earlier, were very promising. nevertheless, the presence of combined nitrogen in some wastewaters can compromise the treatment efficiency. the main goal of this work was to compare plants behaviour in wastewater and in min ...	2009	19559459
selection of a cold-adapted bacterium for bioremediation of wastewater at low temperatures.	amongst more than 1000 isolates collected in various cold environments, the strain arthrobacter psychrolactophilus sp 31.3 has been selected for its ability to grow and to produce exoenzymes at low temperatures, its inability to grow at 37 degrees c, its non-halophilic character and its growth versatility on various media. this non-pathogenic strain displays a strong resistance to desiccation and storage at room temperature and is suitable for the production of freeze-dried bacterial starters. w ...	2009	19578929
production of acrylic acid from acrylonitrile by immobilization of arthrobacter nitroguajacolicus zjutb06-99.	immobilized cells of arthrobacter nitroguajacolicus zjutb06-99 capable of producing nitrilase was used for biotransformation of acrylonitrile to acrylic acid. six different entrapment matrixes were chosen to search for a suitable support in terms of nitrilase activity. ca-alginate proved to be more advantageous over other counterparts in improvement of the biocatalyst activity and bead mechanical strength. the effects of sodium alginate concentration, cacl2 concentration, bead diameter, and rati ...	2009	19597316
arthrobacter psychrochitiniphilus sp. nov., a psychrotrophic bacterium isolated from antarctica.	a psychrotrophic arthrobacter strain, gp3(t), was isolated from adélie penguin guano from antarctica and characterized. the 16s rrna gene sequence of the novel strain showed the highest similarity (97.8 %) with that of arthrobacter psychrolactophilus b7(t). the novel strain showed a morphological change from rod to coccus. the growth temperature range of strain gp3(t) was 0-25 degrees c, with optimal growth at 20 degrees c. the strain grew over a salinity range of between 0 and 3 % (w/v) nacl an ...	2009	19625417
a new cold-adapted beta-d-galactosidase from the antarctic arthrobacter sp. 32c - gene cloning, overexpression, purification and properties.	the development of a new cold-active beta-d-galactosidases and microorganisms that efficiently ferment lactose is of high biotechnological interest, particularly for lactose removal in milk and dairy products at low temperatures and for cheese whey bioremediation processes with simultaneous bio-ethanol production.	2009	19631003
monitoring enzyme catalysis in the multimeric state: direct observation of arthrobacter 4-hydroxybenzoyl-coenzyme a thioesterase catalytic complexes using time-resolved electrospray ionization mass spectrometry.	the ability to examine real-time reaction kinetics for multimeric enzymes in their native state may offer unique insights into understanding the catalytic mechanism and its interplay with three-dimensional structure. in this study, we have used a time-resolved electrospray mass spectrometry approach to probe the kinetic mechanism of 4-hydroxybenzoyl-coenzyme a (4-hba-coa) thioesterase from arthrobacter sp. strain su in the millisecond time domain. intact tetrameric complexes of 4-hba-coa thioest ...	2009	19635449
isolation and identification of novel microcystin-degrading bacteria.	of 31 freshwater bacterial isolates screened using the biolog mt2 assay to determine their metabolism of the microcystin lr, 10 were positive. phylogenetic analysis (16s rrna) identified them as arthrobacter spp., brevibacterium sp., and rhodococcus sp. this is the first report of microcystin degraders that do not belong to the proteobacteria.	2009	19734339
the influence of host-guest inclusion complex formation on the biotransformation of cortisone acetate delta(1)-dehydrogenation.	an intensive and systematic investigation had been carried out on the delta(1)-dehydrogenation of cortisone acetate (ca) to prednisone acetate (pa) by arthrobacter simplex tccc 11037 in the presence of native and modified beta-cyclodextrins (beta-cds). the biotransformation was improved through the formation of the host-guest inclusion complex between ca and cds in aqueous solution. the inclusion complexes of cds with ca were investigated by means of phase solubility, 2d nmr spectroscopy and dif ...	2009	19744560
high-level chromate resistance in arthrobacter sp. strain fb24 requires previously uncharacterized accessory genes.	the genome of arthrobacter sp. strain fb24 contains a chromate resistance determinant (crd), consisting of a cluster of 8 genes located on a 10.6 kb fragment of a 96 kb plasmid. the crd includes chra, which encodes a putative chromate efflux protein, and three genes with amino acid similarities to the amino and carboxy termini of chrb, a putative regulatory protein. there are also three novel genes that have not been previously associated with chromate resistance in other bacteria; they encode a ...	2009	19758450
[catalytical properties of arthrobacter nicotianae cells, a producer of glucose isomerase, immobilized in xerogel of silicium dioxide].	arthrobacter nicotinanae cells, producers of glucose isomerase, were immobilized in xerogel of silicium dioxide, and properties of the resulted heterogeneous biocatalysts were investigated in the process of isomerization of monosaccharide (glucose and fructose). the glucose isomerase activity of the resulted biocatalysts was shown to be 10 u/g, on average, taking into account the loss of the activity upon the immobilization, which amounted to 50% of the cell activity in suspension. the rate of t ...	2009	19764612
a new beta-galactosidase with a low temperature optimum isolated from the antarctic arthrobacter sp. 20b: gene cloning, purification and characterization.	a psychrotrophic bacterium producing a cold-adapted beta-galactosidase upon growth at low temperatures was classified as arthrobacter sp. 20b. a genomic dna library of strain 20b introduced into escherichia coli top10f' and screening on x-gal (5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside)-containing agar plates led to the isolation of beta-galactosidase gene. the beta-galactosidase gene (bgas) encoding a protein of 1,053 amino acids, with a calculated molecular mass of 113,695 kda. analys ...	2009	19771412
[biodehydrogenation of 11beta-hydroxyl melroxyprogesterone by arthrobacter simplex ur016 in microemulsion system].	to improve mass transfer and enhance the yield for c(1,2) biodehydrogenation of steroid 11beta-hydroxyl medroxyprogesterone, we carried out the dehydrogenation reaction of 11beta-hydroxyl medroxyprogesterone in an oil-in-water (o/w) microemulsion by arthrobacter simplex ur016. we studied the effects of system composition, dehydrogenation temperature and substrate concentration on microbial transformation. we formulated a suitable o/w microemulsion system with arthrobacter simplex ur016 culture b ...	2009	19777818
impact of phenolic substrate and growth temperature on the arthrobacter chlorophenolicus proteome.	we compared the arthrobacter chlorophenolicus proteome during growth on 4-chlorophenol, 4-nitrophenol, or phenol at 5 and 28 degrees c, both for the wild-type and a mutant strain with mass spectrometry based proteomics. a label-free workflow employing spectral counting identified 3749 proteins across all growth conditions, representing over 70% of the predicted genome and 739 of these proteins form the core proteome. statistically significant differences were found in the proteomes of cells grow ...	2009	19714879
atrazine and terbuthylazine mineralization by an arthrobacter sp. isolated from a sugarcane-cultivated soil in kenya.	a tropical soil from a kenyan sugarcane-cultivated field showed a very high capability to mineralize (14)c-ring-labeled atrazine. in laboratory experiments this soil mineralized about 90% of the applied atrazine within 98 d. the atrazine-degrading microbial community was enriched in liquid cultures containing atrazine as the sole n source and 100 mgl(-1) glucose as additional c source. from the enrichment culture a bacterial strain was isolated and identified by comparative sequence analysis of ...	2009	19674769
the presence of embedded bacterial pure cultures in agar plates stimulate the culturability of soil bacteria.	traditional methods for bacterial cultivation recover only a small fraction of bacteria from all sorts of natural environments, and attempts have been made to improve the bacterial culturability. here we describe the development of a cultivation method, based on the embedment of pure bacterial cultures in between two layers of agar. plates containing either embedded pseudomonas putida or arthrobacter globiformis resulted in higher numbers of cfus of soil bacteria (21% and 38%, respectively) afte ...	2009	19699242
genetic diversity of plant growth promoting rhizobacteria isolated from rhizospheric soil of wheat under saline condition.	in this study, a total of 130 rhizobacteria was isolated from a saline infested zone of wheat rhizosphere, and screened for plant growth promoting (pgp) traits at higher salt (nacl) concentrations (2, 4, 6, and 8%). the results revealed that 24 rhizobacterial isolates were tolerant at 8% nacl. although all the 24 salt tolerable isolates produced indole-3-acetic acid (iaa), while 10 isolates solubilized phosphorus, eight produced siderophore, and six produced gibberellin. however, only three isol ...	2009	19701667
eric-pcr-based strain-specific detection of phenol-degrading bacteria in activated sludge of wastewater treatment systems.	to evaluate the use of enterobacterial repetitive intergenic consensus pcr (eric-pcr)-derived probes and primers to specifically detect bacterial strains in an activated sludge microbial community.	2009	19708883
[dormant form of micrococcus luteus and arthrobacter globiformis, not growing on standard media].		2009	19827710
analysis of two gene clusters involved in the degradation of 4-fluorophenol by arthrobacter sp. strain if1.	arthrobacter sp. strain if1 is able to grow on 4-fluorophenol (4-fp) as a sole source of carbon and energy. to clone the 4-fp degradation genes, dna libraries were constructed and screened with a probe obtained by pcr using primers designed on the basis of conserved regions of aromatic two-component monooxygenases. sequencing of positive clones yielded two gene clusters, each harboring a gene encoding a monooxygenase with high sequence similarity to the oxygenase component of 4-nitrophenol and 4 ...	2009	19837837
[genetic diversity of cultivable bacteria of dominant plants in a potassium mine tailing of nanjing].	plant rhizosphere bacteria play an important role in biogeochemical cycles. microbial diversity of cultivable bacteria from soil-plant-mineral system in a mine tailing was assessed.	2009	19873749
c and n isotope fractionation suggests similar mechanisms of microbial atrazine transformation despite involvement of different enzymes (atza and trzn).	transformation of atrazine to hydroxyatrazine in the environment may be underestimated by current assessment schemes since immobilization and further transformation of the metabolite can render parent-to-daughter compound ratios unreliable. this study reports significant c and n isotope fractionation of atrazine in transformation to hydroxyatrazine by chelatobacter heintzii, pseudomonas sp. adp, and arthrobacter aurescens tc1 highlighting an alternative approach to detecting this natural transfo ...	2009	19924926
[isolation and identification of four dbp-degrading strains and molecular cloning of the degradation genes].	four di-butyl-phthalate(dbp)-degrading bacterial strains, jdc-1, jdc-8, jdc-9 and jdc-12, were isolated from soil. the strains were gram positive. the 16s rrna sequence analysis revealed that the four strains had similarities of 99% with arthrobacter sp.. according to the morphologic, physiobiochemical characteristics and the analysis of their 16s rrna, all the four strains were identified as arthrobacter sp.. a 900 bp dna fragment was obtained from the four strains by pcr amplified and clone. w ...	2009	19927831
16s rrna gene-based identification of cultured bacterial flora from host-seeking ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks, vectors of vertebrate pathogens.	a total of 151 bacterial isolates were recovered from different developmental stages (larvae, nymphs and adults) of field-collected ticks (67 strains from ixodes ricinus, 38 from dermacentor reticulatus, 46 from haemaphysalis concinna). microorganisms were identified by means of 16s rrna gene sequencing. almost 87 % of the strains belonged to g(+) bacteria with predominantly occurring genera bacillus and paenibacillus. other g(+) strains included arthrobacter, corynebacterium, frigoribacterium, ...	2009	19937215
antagonistic activity of bacteria isolated from crops cultivated in a rotation system and a monoculture against pythium debaryanum and fusarium oxysporum.	the effect of crop rotation and monocropping on the occurrence of bacteria with antagonistic activity toward pythium debaryanum and fusarium oxysporum was shown. arthrobacter spp., fluorescent pseudomonas spp. and actinomycetes were isolated from winter rape, sugar beet and winter barley rhizosphere and bulk soil from the plots of a long-term crop rotation experiment (18 years). the occurrence of mycoantagonistic isolates and their antibiosis level exhibited specificity for the site, crop and cr ...	2009	19937218
[dynamics of microbial populations in the biofilm on protective coating].	dynamics of microbial populations in the biofilm on the surface of protective coating poliken 980-25 has been studied. it was shown that in the process of succession one can observe some changes of taxonomic composition of biofilm bacteria. bacteria of pseudomonas genus were revealed at early stages of succession, they reduce fe+3 and oxidize carbohydrates. bacteria of arthrobacter genus begin developing 2 hours after the exposure, bacillus genus bacteria appear 6 hours after the experiment begi ...	2009	19938604
[monosaccharide composition of exopolymer complex of bacteria-destructors of protective coatings].	monosaccharide composition of exopolymer complex (epc) ofbacteria-destructors of protective coatings pseudomonas sp. t/, pseudomonas sp. 109, arthrobacter sp. 102 has been studied. it is established that monosaccharide composition of exopolymer complex of bacteria is different and depends on the bacteria growth model. it is mainly represented by penthoses and hexoses. glucose, galactose, arabinose, which are determined in epc of biofilm and plankton of all studied bacteria, are dominating monosa ...	2009	19938612
[lipolytic and catalase activity of bacteria-destructors of protective coatings].	lipolytic and catalase activity of bacteria-destructors of protective coatings at different growth models: biofilm and plankton have been studied. it was shown that the synthesis of exoenzymes in the biofilm is different. activity of extracellular lipase and catalase in the biofilm is 1.1-1.5 and 1.2-2.1 times higher, respectively, than in plankton. the experiment duration being increased to 14 days, the lipolytic activity considerably decreases. activity of studied enzymes, produced by bacteria ...	2009	19938616
a polyphasic study on the species diversity of the sediment microbiota of lake hévíz.	lake hévíz is the largest natural thermal lake of europe, harboring special bacterial communities. the aim of the present study was to gain information about the distribution and species diversity of the sediment microbiota, with special focus on actinobacteria, by using cultivation-based and -independent molecular methods. samples from two depths were taken in two different locations in october 2007. 245 strains were isolated, grouped to 85 otus by ardra, and identified by 16s rdna sequencing. ...	2009	20038486
genetic and phenotypic diversity of parathion-degrading bacteria isolated from rice paddy soils.	three parathion-degrading bacteria and eight pairs of bacteria showing syntrophic metabolism of parathion were isolated from rice field soils, and their genetic and phenotypic characteristics were investigated. the three isolates and eight syntrophic pairs were able to utilize parathion as a sole source of carbon and energy, producing p-nitrophenol as the intermediate metabolite during the complete degradation of parathion. analysis of 16s rrna gene sequence indicated that the isolates were rela ...	2009	20075637
isolation of an isocarbophos-degrading strain of arthrobacter sp. scl-2 and identification of the degradation pathway.	isocarbophos is a widely used organophosphorus insecticide that has caused environmental pollution in many areas. however, degradation of isocarbophos by pure cultures has not been extensively studied, and the degradation pathway has not been determined. in this paper, a highly effective isocarbophos-degrading strain, scl-2, was isolated from isocarbophos-polluted soil. strain scl-2 was preliminarily identified as arthrobacter sp. based on its morphological, physiological, and biochemical proper ...	2009	19996699
identification and characterization of bacteria isolated under selective pressure of volatile organic compounds.	several bacterial strains tolerant to volatile organic compounds (vocs) were isolated from the air emissions of a typical industrial wood painting plant. four strains that grew vigorously on a mineral salt basal medium and voc-saturated atmosphere were identified using biolog and fatty acids analysis. a 16s rdna sequence comparison was also performed on three of the four strains. their phylogenetic positions were respectively: mc8m6 of the genus arthrobacter mcep3 of the genus microbacterium, mc ...	2009	20112870
identification of airborne bacterial and fungal species in the clinical microbiology laboratory of a university teaching hospital employing ribosomal dna (rdna) pcr and gene sequencing techniques.	universal or "broad-range" pcr-based ribosomal dna (rdna) was performed on a collection of 58 isolates (n = 30 bacteria + 28 fungi), originating from environmental air from several locations within a busy clinical microbiology laboratory, supporting a university teaching hospital. a total of 10 bacterial genera were identified including both gram-positive and gram-negative genera. gram-positive organisms accounted for 27/30 (90%) of total bacterial species, consisting of seven genera and include ...	2009	20183192
bacterial diversity from benthic mats of antarctic lakes as a source of new bioactive metabolites.	during the micromat project, the bacterial diversity of microbial mats growing in the benthic environment of antarctic lakes was accessed for the discovery of novel antibiotics. in all, 723 antarctic heterotrophic bacteria belonging to novel and/or endemic taxa in the +¦-, +¦- and +¦-subclasses of the proteobacteria, the bacteroidetes branch, and of the high and low percentage g+c gram-positives, were isolated, cultivated in different media and at different temperatures, and then screened for th ...	2009	21798170
heterologous expression and characterization of choline oxidase from the soil bacterium arthrobacter nicotianae.	in the course of a microbial screening of soil samples for new oxidases, different enrichment strategies were carried out. with choline as the only carbon source, a microorganism was isolated and identified as arthrobacter nicotianae. from this strain, a gene coding for a choline oxidase was isolated from chromosomal dna. this gene named coda was cloned in escherichia coli bl21-gold and the protein (an_coda) heterologously overexpressed as a soluble intracellular protein of 59.1 kda. basic bioch ...	2009	18787818
plasmid as a measure of microbial degradation capacity for 2,4-dichlorophenoxyacetic acid.	the purpose of this research was to pursuit the quantification of microbial degradation capacity for 2,4-dichlorophenoxyacetic acid (2,4-d) by detecting and quantifying a prominent 2,4-d degradation encoding plasmid. batch reactor acclimation, de-acclimation, and re-acclimation tests were conducted during which periods the courses of 2,4-d dissipation and plasmid evolution were quantitatively measured. pure cultures of bacterial strains were detected to give rise to a plasmid approximately the s ...	2009	18930390
liquid chromatography-mass spectrometry to study chondroitin lyase action pattern.	liquid chromatography-mass spectrometry was applied to determine the action pattern of different chondroitin lyases. two commercial enzymes, chondroitinase abc (proteus vulgaris) and chondroitinase acii (arthrobacter aurescens), having action patterns previously determined by viscosimetry and gel electrophoresis were first examined. next, the action patterns of recombinant lyases, chondroitinase abc from bacteroides thetaiotaomicron (expressed in escherichia coli) and chondroitinase ac from flav ...	2009	18992215
endophytic bacterial flora in root and stem tissues of black pepper (piper nigrum l.) genotype: isolation, identification and evaluation against phytophthora capsici.	to isolate and identify black pepper (piper nigrum l) associated endophytic bacteria antagonistic to phytophthora capsici causing foot rot disease.	2009	19018963
treatment of chromium(vi) solutions in a pilot-scale bioreactor through a biofilm of arthrobacter viscosus supported on gac.	the aim of this work is to evaluate the applicability of a biofilm to the removal of chromium in solution, at a pilot scale. the effect of the initial concentration of metal on the biosorption behavior of an arthrobacter viscosus biofilm supported on granular activated carbon, in batch and column essays was also analyzed. six isotherm equations have been tested in the present study. the best fit was obtained with the freundlich model. it was observed that as the initial chromium concentration in ...	2009	18565747
replication-involved genes of pal1, the linear plasmid of arthrobacter nitroguajacolicus rü61a--phylogenetic and transcriptional analysis.	the 113-kb pal1 is the only arthrobacter linear plasmid known; it has terminal inverted repeats and 5' covalently attached terminal proteins (tps). the latter and a telomere-associated protein (tap) are encoded by plasmid orfs 102 and 101, respectively. as for streptomyces linear replicons, in which both above proteins are instrumental in telomere patching, they are involved in pal1 replication as well. however, the alignment of actinobacterial taps and tps revealed that pal1 and the linear elem ...	2010	21185858
studies of dimethylglycine oxidase isoenzymes in arthrobacter globiformis cells.	glycine betaine (gb) could be used by arthrobacter globiformis cells as a sole carbon source. the cells took up this molecule in the low as well as in the high salinity medium. addition of gb to the mineral medium with high salt concentration revealed that gb was also used as an osmoprotectant. dimethylglycine oxidase (dmgo) was involved in the catabolism of gb. two genes for dmgo were detected in a cloned 26267 bp fragment of a. globiformis dna. the genes involved in the tetrahydrofolate-depend ...	2010	21188587
discovery and characterization of d-phenylserine deaminase from arthrobacter sp. tks1.	we discovered a d-phenylserine deaminase that catalyzed the pyridoxal 5'-phosphate (plp)-dependent deamination reaction from d-threo-phenylserine to phenylpyruvate in newly isolated arthrobacter sp. tks1. the enzyme was partially purified, and its n-terminal amino acid sequence was analyzed. based on the sequence information, the gene encoding the enzyme was identified and expressed in escherichia coli. the expressed protein was purified to homogeneity and characterized. the enzyme consisted of ...	2010	21190106
a novel replicative enzyme encoded by the linear arthrobacter plasmid pal1.	the soil bacterium arthrobacter nitroguajacolicus rü61a contains the linear plasmid pal1, which codes for the degradation of 2-methylquinoline. like other linear replicons of actinomycetes, pal1 is characterized by short terminal inverted-repeat sequences and terminal proteins (tppal1) covalently attached to its 5' ends. tppal1, encoded by the pal1.102 gene, interacts in vivo with the protein encoded by pal1.101. bioinformatic analysis of the pal1.101 protein, which comprises 1,707 amino acids, ...	2010	20675469
[effects of nitrogen and phosphorus fertilizer on atrazine degradation and detoxification by degrading strain hb-5].	an atrazine-degrading strain hb-5 was used as a bacteria for biodegradation. treatments of soil with nitrogen single, phosphate single and nitrogen phosphate together with hb-5 were carried out for degradation and eco-toxicity test; then, relationship between atrazine degradation rate and soil available nitrogen, available phosphorus were discussed. atrazine residues were determined by hplc; available nitrogen was determined with alkaline hydrolysis diffusion method; available phosphorus was det ...	2010	21229764
weathering-associated bacteria from the damma glacier forefield: physiological capabilities and impact on granite dissolution.	several bacterial strains isolated from granitic rock material in front of the damma glacier (central swiss alps) were shown (i) to grow in the presence of granite powder and a glucose-nh(4)cl minimal medium without additional macro- or micronutrients and (ii) to produce weathering-associated agents. in particular, four bacterial isolates (one isolate each of arthrobacter sp., janthinobacterium sp., leifsonia sp., and polaromonas sp.) were weathering associated. in comparison to what was observe ...	2010	20525872
influence of ph and inorganic phosphate on toxicity of zinc to arthrobacter sp. isolated from heavy-metal-contaminated sediments.	because of its high solubility over a wide range of ph conditions, zinc is found in many natural and human-impacted systems. zinc speciation is critical in assessing zinc toxicity to microorganisms because it varies considerably with ph and is dependent on other aqueous constituents. combined results of thermodynamic modeling, statistical analysis, and batch culture studies using arthrobacter sp. jm018 suggest that the toxic species may not be solely limited to the free ion, but also includes zn ...	2010	20553043
structural and kinetic studies on the ser101ala variant of choline oxidase: catalysis by compromise.	the oxidation of choline catalyzed by choline oxidase includes two reductive half-reactions where fad is reduced by the alcohol substrate and by an aldehyde intermediate transiently formed in the reaction. each reductive half-reaction is followed by an oxidative half-reaction where the reduced flavin is oxidized by oxygen. here, we have used mutagenesis to prepare the ser101ala mutant of choline oxidase and have investigated the impact of this mutation on the structural and kinetic properties of ...	2010	20561507
high-density polyethylene (hdpe)-degrading potential bacteria from marine ecosystem of gulf of mannar, india.	assessment of high-density polyethylene (hdpe)-degrading bacteria isolated from plastic waste dumpsites of gulf of mannar.	2010	20586938
investigation of mineral water springs of miercurea ciuc (csíkszereda) region (romania) with cultivation-dependent microbiological methods.	water samples of ten mineral water springs at miercurea ciuc (csíkszereda) region (romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. the results of standard hygienic bacteriological tests showed that the hargita spring had perfect and five other springs had microbiologically acceptable water quality (zsögöd-, nagy-borvíz-, taploca-, szentegyháza- and lobogó springs). the water of borsáros spring was exceptionable (high germ count, presence of enterococc ...	2010	20587384
variability of sediment-contact tests in freshwater sediments with low-level anthropogenic contamination--determination of toxicity thresholds.	freshwater sediments with low levels of anthropogenic contamination and a broad range of geochemical properties were investigated using various sediment-contact tests in order to study the natural variability and to define toxicity thresholds for the various toxicity endpoints. tests were performed with bacteria (arthrobacter globiformis), yeast (saccharomyces cerevisiae), nematodes (caenorhabditis elegans), oligochaetes (lumbriculus variegatus), higher plants (myriophyllum aquaticum), and the e ...	2010	20594629
prevalence of the gene trzn and biogeographic patterns among atrazine-degrading bacteria isolated from 13 colombian agricultural soils.	the following study evaluated the diversity and biogeography of 83 new atrazine-degrading bacteria and the composition of their atrazine degradation genes. these strains were isolated from 13 agricultural soils and grouped according to rep-pcr genomic fingerprinting into 11 major clusters, which showed biogeographic patterns. three clusters (54 strains) belonged to the genus arthrobacter, seven clusters (28 strains) were similar to the genus nocardioides and only one strain was a gram-negative f ...	2010	20597985
study on the removal of indoor vocs using biotechnology.	with the improvement of living standard and civil building innovation, large numbers of building materials and decoration are being used. the emission of gaseous organic pollutants such as formaldehyde, benzene, toluene and xylene from these materials may trigger immediate or persistent potential harm to human health. in this study, with the combination of bake-out exhaust, biological treatment was applied to deal with volatile organic compounds (vocs). four bacterial strains were isolated from ...	2010	20598440
chemical composition and antibacterial activity of essential oils of lantana camara, ageratum houstonianum and eupatorium adenophorum.	essential oils have applications in folk medicine, food preservation, and as feed additives. the essential oils of lantana camara linn. (verbenaceae), ageratum houstonianum mill. (asteraceae) and eupatorium adenophorum spreng. (asteraceae) were analyzed by gas chromatography-mass spectrometry (gcms). in l. camara oil, of the total identified (83.91%) volatile constituents, five constituents [3,7,11-trimethyl-1,6,10-dodecatriene (28.86%), beta-caryophyllene (12.28%), zingiberene (7.63%), gamma-cu ...	2010	20645797
[diversity of halophilic and halotolerant bacteria isolated from non-saline soil collected from xiaoxi national natural reserve, hunan province].	to investigate the diversity of cultivable halophilic and halotolerant bacteria isolated from ordinary non-saline soil samples collected from xiaoxi national natural reserve (28 degrees 42' 15"-28 degrees 53' 15" n, 110 degrees 6' 50"-110 degrees 21' 35" e), hunan province, china.	2010	21268889
[isolation, identification and soil remediation of atrazine-degrading strain t3 ab1].	to provide new atrazine-degrading strains for atrazine-polluted soil, we isolated the high-efficiency degradation bacterium from contaminated soil, identified with taxonomy, and studied the degrading characteristics and remediation capability of the strain in black soil.	2010	21365918
characterization of a bacterial community from a northeast siberian seacoast permafrost sample.	a combination of culture-dependent and -independent techniques was used to characterize a bacterial community, examine cold adaptation of isocitrate lyase (icl) genes, and detect genes with important ecological functions in a permafrost sample from the bykovsky peninsula on the laptev sea coast of northeast siberia. according to the 16s rrna gene sequence, 47 of the cultured isolates were members of the phyla firmicutes, proteobacteria, and actinobacteria, with 85% of the isolates belonging to t ...	2010	20695892
engineering host cell lines to reduce terminal sialylation of secreted antibodies.	covalently-linked glycans on proteins have many functional roles, some of which are still not completely understood. antibodies have a very specific glycan modification in the fc region that is required for mediating immune effector functions. these fc glycans are typically highly heterogeneous in structure, and this heterogeneity is influenced by many factors, such as type of cellular host and rate of ab secretion. glycan heterogeneity can affect the fc-dependent activities of antibodies. it ha ...	2010	20716959
isolation and characterization of hexavalent chromium-reducing rhizospheric bacteria from a wetland.	scirpus americanus pers. occurs naturally in "san germán," a pond that serves as a receptor of industrial wastewater in guanajuato, méxico. this plant accumulates metals mainly in the root: concentrations (mg/kg) of cr, as, cd and se were 970, 49, 41, and 85 respectively. analysis of rhizosphere samples indicated bacterial population of 10(8) cfu g(-1) in media with 0.2 mm cr(vi) and 10 mm sodium gluconate. thirteen isolates were obtained and phylogenetic analyses (16s rrna) indicated they corre ...	2010	20734910
diversity of cold-active protease-producing bacteria from arctic terrestrial and marine environments revealed by enrichment culture.	a new approach for enrichment culture was applied to obtain cold-active protease-producing bacteria for marine and terrestrial samples from svalbard, norway. the method was developed for the enrichment of bacteria by long-term incubation at low temperatures in semi-solid agar medium containing meat pieces as the main source of carbon and energy. zobell and 0.1x nutrient broth were added for marine and terrestrial microorganisms, respectively, to supply basal elements for growth. one to three typ ...	2010	20799082
isolation and characterization of a pseudomonas sp. strain iitr01 capable of degrading α-endosulfan and endosulfan sulfate.	to isolate bacteria capable of degrading endosulfan (es) and the more toxic es sulfate and to characterize their metabolites.	2010	20825519
novel isonitrile hydratase involved in isonitrile metabolism.	we previously discovered n-substituted formamide deformylase (nfda) in arthrobacter pascens f164, which degrades n-substituted formamide (fukatsu, h., hashimoto, y., goda, m., higashibata, h., and kobayashi, m. (2004) proc. natl. acad. sci. u.s.a. 101, 13726-13731). in this study, we found an enzyme involved in the first step of isonitrile metabolism, isonitrile hydratase, that hydrates isonitrile to the corresponding n-substituted formamide. first, we investigated the optimum culture conditions ...	2010	20826798
phytoremediation of mercury in pristine and crude oil contaminated soils: contributions of rhizobacteria and their host plants to mercury removal.	the rhizospheric soils of three tested legume crops: broad beans (vicia faba), beans (phaseolus vulgaris) and pea (pisum sativum), and two nonlegume crops: cucumber (cucumis sativus) and tomato, (lycopersicon esculentum) contained considerable numbers (the magnitude of 10(5)g(-1) soil) of bacteria with the combined potential for hydrocarbon-utilization and mercury-resistance. sequencing of the 16s rrna coding genes of rhizobacteria associated with broad beans revealed that they were affiliated t ...	2010	20833430
the catalytic efficiency of trehalose-6-phosphate synthase is effected by the n-loop at low temperatures.	the enzyme otsa (trehalose-6-phosphate synthase) is ubiquitous in both prokaryotic and eukaryotic organisms, where it plays a critical role in stress resistance and glucose metabolism. here, we cloned the otsa gene from arthrobacter sp. cjts, and expressed and then purified the recombinant proteins. enzyme activity analysis indicated that the high catalytic efficiency of otsa from arthrobacter sp. cjts resulted from the high affinity of the enzyme for uridine 5'-diphosphoglucose (udp-glc) at low ...	2010	20838774
arthrobacter equi sp. nov., a novel bacterium isolated from a veterinary clinical material.	a gram-positive, aerobic, catalase-positive, non-spore-forming, rod-shaped bacterium, strain immib l-1606t, isolated from genital swabs of a horse was characterized using a polyphasic approach. comparative 16s rrna gene sequence analysis showed that the organism was related to the genus arthrobacter, displaying 93.5 - 99.1 % sequence similarities with established species of the genus. cell wall analysis revealed cell-wall peptidoglycan type a3α l-lys - l-ser - l-thr - l-ala. the results of dna-d ...	2010	20870884
[xylose isomerase constitutive biosynthesis in arthrobacter nicotianae].	the specific features of biosynthesis of the cell-bound xylose isomerase by the actinobacterium arthrobacter nicotianae bim v-5 were studied. it was demonstrated that the constitutive synthesis of this enzyme in the studied bacteria, not subject to catabolite repression, was inhibited by xylulose, an intermediate product ofxylose utilization and the final product of its enzymatic isomerization. short-term experiments demonstrated that xylulose at a concentration of 0.005% almost completely repre ...	2010	20873168
bacterial community diversity in the brazilian atlantic forest soils.	the aim of this study was to characterize the bacterial community diversity of the brazilian atlantic forest soil by means of both cultivation and 16s rrna clone libraries. a collection of 86 representative isolates, obtained from six samples of atlantic forest soils from the national park of serra dos órgãos (parnaso), belonged to the genera arthrobacter, bacillus, burkholderia, leifsonia, paenibacillus, pseudomonas, ralstonia, serratia, and streptomyces according to the 16s rrna sequences. rep ...	2010	20886336
identification of chitinases is-chia and is-chib from isoptericola jiangsuensis clg and their characterization.	a 274-bp conserved fragment of chia (chia-cf) was amplified from the genomic dna of isoptericola jiangsuensis clg (dsm 21863, cctcc ab208287) using the specific pcr primers. based on chia-cf sequences, a 5233-bp dna fragment was obtained by self-formed adaptor pcr. dna sequencing analysis revealed there were two contiguous open reading frames coding for the precursors of is-chia [871 amino acids (aa)] and is-chib (561 aa) in the 5233-bp dna fragment. the is-chia and is-chib exhibited 58% and 62% ...	2010	20922373
purification and characterization of inulin fructotransferase (dfa iii-forming) from arthrobacter aurescens sk 8.001.	the soil bacterium arthrobacter aurescens sk 8.001 produces inulin fructotransferase (iftase), and liquid chromatography-mass spectrometry (lc-ms) and carbon-13 nuclear magnetic resonance (13c nmr) analysis demonstrated that the main product of the enzyme was difructose anhydride iii (dfa iii). the iftase was purified by ethanol precipitation, deae sepharose fast flow, and superdex 200 10/300 gl gel chromatography. its molecular mass was estimated to be 40 kda by sds-page and 35 kda by gel filtr ...	2010	20933390
appraising bacterial strains for rapid bod sensing--an empirical test to identify bacterial strains capable of reliably predicting real effluent bods.	the measured response of rapid biochemical oxygen demand (bod) biosensors is often not identical to those measured using the conventional 5-day bod assay. this paper highlights the efficacy of using both glucose-glutamic acid (gga) and organisation for economic cooperation and development (oecd) bod standards as a rapid screen for microorganisms most likely to reliably predict real effluent bods when used in rapid bod devices. using these two synthetic bod standards, a microorganism was identifi ...	2010	20938773
diversity and activity of cultivable aerobic planktonic bacteria of a saline lake located in sovata, romania.	aerobic bacterial strains from the salt water of lake red (sovata, romania) were cultivated. more than half of the 80 strains were g(-) and formed motile straight rods. only a few strains produced acid from d-glucose and reduced nitrate to nitrite. optimum nacl concentration for growth varied between 5 and 15 % in the majority of the strains, so the isolates were regarded moderately halophilic. on the basis of the 16s rrna gene sequence similarity almost half of the strains were identified as me ...	2010	20941581
direct cellular lysis/protein extraction protocol for soil metaproteomics.	we present a novel direct protocol for deep proteome characterization of microorganisms in soil. the method employs thermally assisted detergent-based cellular lysis (sds) of soil samples, followed by tca precipitation for proteome extraction/cleanup prior to liquid chromatography-mass spectrometric characterization. this approach was developed and optimized using different soils inoculated with genome-sequenced bacteria (gram-negative pseudomonas putida or gram-positive arthrobacter chloropheno ...	2010	20954746
transgenic tobacco plants expressing atza exhibit resistance and strong ability to degrade atrazine.	atrazine chlorohydrolase (atza) catalyzes hydrolytic dechlorination and can be used in detoxification of atrazine, a herbicide widely employed in the control of broadleaf weeds. in this study, to investigate the potential use of transgenic tobacco plants for phytoremediation of atrazine, atza genes from pseudomonas sp. strain adp and arthrobacter strain ad1 were transferred into tobacco. three and four transgenic lines, expressing atza-adp and atza-ad1, respectively, were produced by agrobacteri ...	2010	20960204
16s rdna sequence analysis of culturable marine biofilm forming bacteria from a ship's hull.	marine bacteria from the hull of a ship in the form of biofilms or microfouling were isolated, cultured, and identified by phylogenetic analysis using 16s rdna sequences. with an average length of 946 bp, all the 16 sequences were classified using the ribosomal database project (rdp) and were submitted to the national center for biotechnology information. phylogenetic analysis using 16s rdna sequences indicated that the 16 strains belonged to the firmicutes (ik-mb6 exiguobacterium aurantiacum, i ...	2010	21038149
transgenic brassica chinensis plants expressing a bacterial coda gene exhibit enhanced tolerance to extreme temperature and high salinity.	transgenic brassica compestris l. spp. chinensis plants expressing a choline oxidase (coda) gene from arthrobacter globiformis were obtained through agrobacterium tumefaciens-mediated transformation. in the transgenic plants, coda gene expression and its product transportation to chloroplasts were detected by the enzyme-linked immunosorbent assay (elisa) examination, immunogold localization, and (1)h-nuclear magnetic resonance ((1)h-nmr). stress tolerance was evaluated in the t(3) plants under e ...	2010	21043054
biodegradability of bacterial surfactants.	this work aimed at evaluating the biodegradability of different bacterial surfactants in liquid medium and in soil microcosms. the biodegradability of biosurfactants by pure and mixed bacterial cultures was evaluated through co(2) evolution. three bacterial strains, acinetobacter baumanni lbbma es11, acinetobacter haemolyticus lbbma 53 and pseudomonas sp. lbbma 101b, used the biosurfactants produced by bacillus sp. lbbma 111a (mixed lipopeptide), bacillus subtilis lbbma 155 (lipopeptide), flavob ...	2010	21053055
optimization of electroporation conditions for arthrobacter with plasmid part2.	a prerequisite for genetic studies of arthrobacter is a high efficiency transformation system that allows for dna transfer, transposon mutagenesis, and expression of specific genes. in this study, we develop a detailed electroporation method through a systematic examination of the factors involved in the entire electroporation process. key features of this procedure, including the addition of penicillin to cells during the early log phase of growth and the presence of 0.5m sorbitol in the electr ...	2010	21078345
evaluation of the ltq-orbitrap mass spectrometer for the analysis of polymerase chain reaction products.	we have investigated the potential and robustness of the off-line coupling of polymerase chain reaction (pcr) with electrospray ionization mass spectrometry (esi-ms), for further applications in the screening of single-nucleotide polymorphisms (snps). this was based on recently reported data demonstrating that anion-exchange solid-phase extraction was the most efficient technique for efficiently desalting pcr products, with a recovery of ∼70%. results showed that this purification approach effic ...	2010	21080500
root exudates modify bacterial diversity of phenanthrene degraders in pah-polluted soil but not phenanthrene degradation rates.	to determine whether the diversity of phenanthrene-degrading bacteria in an aged polycyclic aromatic hydrocarbon (pah) contaminated soil is affected by the addition of plant root exudates, dna stable isotope probing (sip) was used. microcosms of soil with and without addition of ryegrass exudates and with ¹³c-labelled phenanthrene (phe) were monitored over 12 days. phe degradation was slightly delayed in the presence of added exudate after 4 days of incubation. after 12 days, 68% of added phe di ...	2010	21087382
complete biodegradation of 4-fluorocinnamic acid by a consortium comprising arthrobacter sp. strain g1 and ralstonia sp. strain h1.	a consortium of the newly isolated bacterial strains arthrobacter sp. strain g1 and ralstonia sp. strain h1 utilized 4-fluorocinnamic acid for growth under aerobic conditions. strain g1 converted 4-fluorocinnamic acid into 4-fluorobenzoic acid and used the two-carbon side chain for growth, with some formation of 4-fluoroacetophenone as a dead-end side product. in the presence of strain h1, complete mineralization of 4-fluorocinnamic acid and release of fluoride were obtained. degradation of 4-fl ...	2010	21097599
lactulose biosynthesis by β-galactosidase from a newly isolated arthrobacter sp.	lactulose, a ketose disaccharide, is used in both pharmaceutical and food industries. this study was undertaken to screen and isolate potent β-galactosidase-producing bacteria and to evaluate their enzymatic production of lactulose. soil samples from fruit gardens were collected. one isolate designated las was identified whose cell extract could convert lactose and fructose into lactulose. the 16s rdna gene analysis of las revealed its phylogenetic relatedness to arthrobacter sp. the β-galactosi ...	2010	21104424
degradative potential of stenotrophomonas strain hpc383 having genes homologous to dmp operon.	a strain, stenotrophomonas hpc383 is isolated from effluent treatment plant treating wastewater from pesticide industry; degrades various aromatic compounds (cresols, phenol, catechol, 4methyl-catechol and hydroquinone) and crude oil, as determined through hplc and gc analysis. culture hpc383 could degrade (%) various compounds (1 mm) from a mixture: phenol - 99, p-cresol - 100, 4-methylcatechol - 96 and hydroquinone - 43 within 48 h of incubation, whereas it took 7 days to degrade 94% of 0.5% c ...	2010	21123060
isolation, characterization, and use for plant growth promotion under salt stress, of acc deaminase-producing halotolerant bacteria derived from coastal soil.	in total, 140 halotolerant bacterial strains were isolated from both the soil of barren fields and the rhizosphere of six naturally growing halophytic plants in the vicinity of the yellow sea, near the city of incheon in the republic of korea. all of these strains were characterized for multiple plant growth promoting traits, such as the production of indole acetic acid (iaa), nitrogen fixation, phosphorus (p) and zinc (zn) solubilization, thiosulfate (s2o3) oxidation, the production of ammonia ...	2010	21124065
the arthrobacter arilaitensis re117 genome sequence reveals its genetic adaptation to the surface of cheese.	arthrobacter arilaitensis is one of the major bacterial species found at the surface of cheeses, especially in smear-ripened cheeses, where it contributes to the typical colour, flavour and texture properties of the final product. the a. arilaitensis re117 genome is composed of a 3,859,257 bp chromosome and two plasmids of 50,407 and 8,528 bp. the chromosome shares large regions of synteny with the chromosomes of three environmental arthrobacter strains for which genome sequences are available: ...	2010	21124797