Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| clostridial cystitis emphysematosa. | 1974 | 4372771 | |
| the distribution of enzymes and bacteria in the small intestines of slaughter pigs. | 1974 | 4372854 | |
| effect of trypsin, phospholipases, and membrane-impermeable reagents on the uptake of palmitic acid by isolated rat liver cells. | 1974 | 4372944 | |
| non-specific protein binding by adsorbents designed for the specific affinity chromatography of sialidase in crude bacterial extracts. | 1974 | 4373073 | |
| [composition of gases emitted by welchia perfringens and clostridium sporogenes cultivated in different synthetic media]. | 1974 | 4376902 | |
| an inhibitor of phospholipase d in saliva. | 1. bovine, dog and human saliva contain substances which inhibit the soluble phospholipase d present in grass leaf or celery stalk. 2. the inhibitor in bovine saliva is of high molecular weight and exhibits considerable stability to heat, acids and alkalis. 3. the inhibitor has been purified free from salivary mucoprotein. 4. it is suggested that the inhibitor could protect the upper alimentary tract of a herbage-eating animal from the necrotic action of phospholipase d. | 1974 | 4376946 |
| [the induced production of bacteriocin and bacteriophage by the bp6k-n-5 strain of "clostridium perfringens" (author's transl)]. | 1974 | 4377069 | |
| [inhibition of the initiation of the germination of a type clostridium perfringens spores by the bacteriocin from clostridium perfringens bp6k-n5]. | 1974 | 4377122 | |
| relation of enterotoxigenic clostridium perfringens type a to food poisoning. ii. acid exposure and storage conditions affecting enterotoxigenesis of c. perfringens. | 1974 | 4377155 | |
| acute emphysematous cholecystitis. | 1974 | 4377171 | |
| purification and characterization of a sialidase inhibitor, siastatin, produced by streptomyces. | 1974 | 4377572 | |
| clostridium welchii and bacillus cereus infection and intoxication. | 1974 | 4377580 | |
| [infection of surgical wounds with clostridium]. | 1974 | 4377678 | |
| infection in the severely traumatized patient. | 1974 | 4360415 | |
| ultrastructural study of clostridial myositis. | 1974 | 4358598 | |
| isolation of clostridium absonum and its cultural and biochemical properties. | a new procedure for isolation of clostridium absonum was devised. sixtyseven strains of c. absonum were isolated from 135 soil samples, but no strain of c. absonum could be found from human fecal samples. the lecithinase, hemolysin, and lethal toxin in the culture filtrates of this species exhibited low avidity for c. perfringens type a antitoxin. the three activities were inseparable by the present method of purification. a reinvestigation of biochemical properties revealed that incomplete supp ... | 1974 | 4357934 |
| in vivo effects of clostridium perfringens enteropathogenic factors on the rat ileum. | an experimental model was established using the terminal ileum of the rat for characterizing and studying the effects of crude cell-free extract from clostridium perfringens upon physiological and histological parameters involved in the transport process. further work was done with the model system using purified enterotoxin (protein) from the cell extract. using an in vivo perfusion technique it was found that crude extract induces a reversal of net transport, from absorption in controls to sec ... | 1974 | 16558104 |
| rapid microassays for clindamycin and gentamicin when present together and the effect of ph and of each on the antibacterial activity of the other. | gentamicin was measured in the presence of clindamycin by using as the assay organism a strain of staphylococcus aureus that was resistant to clindamycin; clindamycin was measured in the presence of gentamicin by using clostridium perfringens as the assay organism. both assays were in agar diffusion systems, were rapid (2 to 4 h), and had errors of less than 10%. neither antibiotic antagonized or synergized with the other against a variety of organisms. the pk of clindamycin was 7.3, and the pk ... | 1974 | 15828171 |
| antimicrobial susceptibilities of anaerobic bacteria: recent clinical isolates. | minimal inhibitory concentrations of clindamycin, minocycline, metronidazole, penicillin, and carbenicillin were determined by agar dilution against 150 recent clinical isolates of anaerobic bacteria. ninety-nine percent of bacteroides fragilis and all b. melaninogenicus, clostridium perfringens, and fusobacterium were inhibited by clindamycin at 3.1 mug/ml. only 58% of other clostridial species were inhibited by this concentration of clindamycin. minocycline at 3.1 mug/ml inhibited 72% of c. pe ... | 1974 | 15830478 |
| experimental and clinical experience with hyperbaric oxygen in the treatment of clostridial myonecrosis. | at duke university medical center, 49 patients with proved clostridial myonecrosis were treated with hyperbaric oxygen (hbo) during the past 10 years. survival in patients with involvement confined to the extremities was 92.3 percent. survival in patients with combined involvement of extremity and trunk was 53-3 percent, and with primary trunk involvement half of the patients survived. survival for the entire series was 73.5 percent. among the 28 patients receiving at least five hbo treatments, ... | 1975 | 162815 |
| colon carcinogenesis with azoxymethane and dimethylhydrazine in germ-free rats. | the effect of intestinal microflora on the sensitivity of the colon to the carcinogenic effect of azoxymethane and a large dose of 1,2-dimethylhydrazine was studied using germ-free and conventional female fischer rats. injection s. c. of 1,2-dimethylhydrazine-induced tumors of the ear duct, kidney, and small intestine of conventional rats but none in germ-free animals. only 20% germ-free rats showed 1,2-dimethylhydrazine-induced colonic tumors, whereas 93% of conventional rats developed multiple ... | 1975 | 162868 |
| classification of enterotoxins on the basis of activity in cell culture. | two cell culture systems were used in a study of the biological properties of several bacterial enterotoxins in vitro. by means of one model, in which hela cell monolayers were used, cytotoxic effects, interms of detachment of cells from a glass surface due to cell death, were assayed. by means of the second model, activation of the adenyl cyclase-cyclic adenosine 3', 5'-monophosphate (amp) system, in terms of increased steroidogenesis by y-1 adrenal cells (an effect which we have termed cytoton ... | 1975 | 162926 |
| minimal growth requirements for clostridium perfringens and isolation of auxotrophic mutants. | the minimal growth requirements for two strains of clostridium perfringens were defined, and both synthetic and semisynthetic plating media were developed. plate counts of the wild-type strains on both of these minimal media were equivalent to those on complex media. a number of auxotrophic mutants of each strain were isolated, and their phenotypes were defined. | 1975 | 162964 |
| [gas gangrene as a complication of traumatic wounds]. | 1975 | 163054 | |
| composition of the capsular polysaccharides of clostridium perfringens as a basis for their classification by chemotypes. | an analytical procedure, using gas-liquid chromatography, was developed for the identification of the per(trimethylsilyl) ethers of the constituent monosaccharides obtained from the capsular polysaccharides of clostridium perfringens hobbs 5, hobbs 9, hobbs 10, and nctc 10578. qualitative and quantitative differences between the major polysaccharide components enabled the differentiation of the four strains of c. perfringens investigated. | 1975 | 163129 |
| gangliosides of the bovine neurohypophysis. | 1975 | 163296 | |
| [ethiology of infectious enterotexemia of sheep]. | 1975 | 163520 | |
| influence of carbohydrates on growth and sporulation of clostridium perfringens type a. | growth and sporulation of clostridium perfringens type a in duncan and strong (ds) sporulation medium was investigated. a biphasic growth response was found to be dependent on starch concentration. maximal levels of heat-resistant spores were formed at a starch concentration of 0.40%. addition of glucose, maltose, or maltotriose to a sporulating culture resulted in an immediate turbidity increase, indicating that biphasic growth in ds medium may be due to such starch degradation products. amylos ... | 1975 | 163617 |
| antigenic relationship between medullary thymocytes and a subpopulation of peripheral t cells in the rat: description of a masked antigen. | 1975 | 163704 | |
| superoxide dismutase in some obligately anaerobic bacteria. | 1975 | 163764 | |
| analysis of unidirectional fluxes of sodium during diarrhea induced by clostridium perfringens enterotoxin in the rat terminal ileum. | net intestinal transport of sodium in vivo, in control and enterotoxin (clostridium perfringens)-treated rats, was resolved into two unidirectional fluxes, influx from and efflux into the lumen of the terminal ileum. in rats treated with the toxin, sodium influx remained similar to control values even during fluid and electrolyte loss to the lumen. net loss of sodium was shown to be due to nearly a twofold increase in sodium efflux to the lumen in toxin-treated animals. there was only slight his ... | 1975 | 163797 |
| enterotoxin formation by different toxigenic types of clostridium perfringens. | sixty-nine strains of clostridium perfringens of different toxigenic types were investigated for enterotoxin production. enterotoxin was definitively detected only in strains of types a and c. this is the first report where enterotoxin production has been demonstrated in a toxigenic type other than type a. the exterotoxin-positive type c strains were isolated from cases of enteritis necroticans ("pig bel+) in new guinea. the major enterotoxin from type c showed a reaction of complete identity wi ... | 1975 | 163799 |
| immunological comparison of various human pregnancy-associated plasma proteins. | direct immunodiffusion comparison with specific antisera demonstrated that all of the four pregnancy-associated plasma proteins (papps) described in our laboratory are distinct from the pregnancy zone protein (von schoultz); alpha2-pregnoglobulin (berne); pregnancy-associated alpha2-glycoprotein (sp3) (bohn); new serum alpha2-macroglobulin (stimson); pag (horne); pregnancy-associated alpha2-globulin (kasukawa); pal (mclaren), and xh protein (dunston). all the latter proved to be immunologically ... | 1975 | 163802 |
| susceptibility of anaerobic bacteria to metronidazole: relative resistance of non-spore-forming gram-positive baccilli. | susceptibility of 358 clinical isolates of obligate anaerobes to metronidazole was determined by an agar-dilution technique. only 66% of all isolates were inhibited by 6.25 mug/ml, whereas 30% required larger than or equal to 50 mug/ml. considerable variation in susceptibility was observed among different genera and species of bacteria. fusobacterium was most senstitive, followed by clostridium, bacteroides and peptococcus, peptostreptococcus, veillonella and acidaminococcus, and non-spore-formi ... | 1975 | 163867 |
| asymmetry of influenza virus membrane bilayer demonstrated with phospholipase c. | 1975 | 163976 | |
| the relation between the rabbit potency test and the response of sheep to sheep clostridial vaccines. | six commercially available clostridial vaccines comprising one oil-emulsion, two alum-precipitated and three aluminum hydroxide adjuvanted preparations, each containing between two and seven antigenic components, were administered to groups of 10 rabbits and eight sheep in accordance with manufacturers' recommendations. serum antitoxic values to cl welchii beta, cl welchii epsilon, cl septicum, cl oedematins and cl tetani toxins were determined 14 days after completion of each vaccination course ... | 1975 | 164058 |
| antitoxin titer in colostrum and milk after vaccination of sows with clostridium perfringens type c toxoid vaccine. | 1975 | 164076 | |
| an outbreak of c welchii type c enterotoxaemia in young lambs in south west scotland. | 1975 | 164082 | |
| effects of proteases and neuraminidase on rbc surface charge and agglutination. a kinetic study. | electrophoretic mobility, membrane sialic acid content and agglutinability by "incomplete" antisera against rh-o, hr' and k antigens were determined for red blood cells in the course of treatment with trypsin, ficin and neuraminidase. neuraminidase gradually produces a slight to moderate agglutinability as it reduced surface charge density in proportion to the amount of sialic acid removed. proteases acted in two distinct steps. the first stage is characterized by the cells rapidly becoming high ... | 1975 | 164087 |
| interaction of 4,5-dibromo-2,7-di-(acetatomercuri)-fluorescein with dnas of different base composition. | the changes in absorption spectra in the visible region observed on adding different naturally occurring and synthetic dna duplexes to solutions of 4,5-dibromo-2,7-di-(acetatomercuri)-fluorescein indicate that the mercurial reacts with polynucleotides of this type. the reaction is reversible as proved by adding excess of kcn which restores the original spectra of the free dye. the interaction is characterised also by quenching of the fluorescence of the dye and the induction of optical activity ... | 1975 | 164231 |
| changes in phosoholipid susceptibility toward phospholipases induced by atp depletion in avian and amphibian erythrocyte membranes. | about half of the sphingomyelin content of fresh and atp-depleted chicken erythrocytes is hydrolysed by sphingomyelinase. removal of spingomyelin exposes the rest of the membrane phospholipids to hydrolysis by phospholipase c only in atp-depleted but not in fresh cells. addition of both sphinogomyelinase and phospholipase c to atp-depleted cells causes about 60-70 percent hydrolysis of the total phospholipids accompanied by extensive (90 percent) hemolysis. the phospholipids of toad erythrocytes ... | 1975 | 164239 |
| determination of toxin-induced leakage of different-size nucleotides through the plasma membrane of human diploid fibroblasts. | human diploid lung fibroblasts were treated with cytolytic bacterial toxins and the nature of the membrane damage was investigated. [3h] uridine was used for differential labeling of cytoplasmic components of small or large molecular size. two principal size categories were achieved by labeling the fibroblasts in either early growth phase or stationary phase, a high-molecular weight ribonucleic acid label and a low-molecular-weight nucleotide label. the size of the labeled molecules was determin ... | 1975 | 164404 |
| characterization of enterotoxin purified from clostridium perfringens type c. | enterotoxin produced by a sporulating culture of clostridium perfringens type c, which had been isolated from a case of severe necrotic enteritis, was purified. the molecular weight was estimated to be 36,000 by gel chromatography on sephadex g-100 and 33,400 by ultracentrifugation. the sedimentation coefficient s20,w was 2.92. the toxin protein exhibited unusual behavior on sodium dodecyl sulfate gels, and toxin aggregates having molecular weights of 68,000, 85,000, 105,000, and 140,000 were ob ... | 1975 | 164411 |
| the compatibility of netropsin and actinomycin binding to natural deoxyribonucleic acid. | the simultaneous binding of netropsin and actinomycin to four natural dnas was studied to determine the influence of one ligand on the binding of the other. actinomycin binds specifically to gc sites, whereas netropsin binds specifically to at sites. spectral titrations, thermal denaturation, and analytical buoyant density centrifugation were employed to measure the binding interference of these drugs. the binding of actinomycin to dna was decreased by the presence of netropsin. increasing the g ... | 1975 | 164447 |
| catabolism of photo-oxidized and desialylated hemopexin in the rabbit. | following injection of rabbit 125i-asialohemopexin, more than 90% of the protein-bound 125i was removed from the circulation of rabbits within 12 min. the amount of asialoprotein in the catabolic compartment reached a peak concentration (75 to 85%) 12 min after injection and was completely eliminated from this compartment within 2 hours. the degradation products were excreted into the urine, with 50 to 70% of the 125i eliminated during the first 24 hours and 90 to 95% excreted by 48 hours. analy ... | 1975 | 164457 |
| isolation of free brown and white fat cells. | 1975 | 164607 | |
| prophylactic antibiotics in cesarean section. | using a random selection procedure, 100 patients were evaluated in order to determine the value of prophylactic antibiotics in cesarean section. all patients were evaluated for febrile morbidity, and specific infection sites were recorded. significant differences were found between the treated and control groups and between cesarean sections performed prior to the onset of labor and after the onset of labor. | 1975 | 164642 |
| analysis of homozygous serum alpha1-antitrypsins: effects of neuraminidase. | 1975 | 164647 | |
| [use of a synthetic medium for cultivating pathogenic anaerobes]. | it was shown that a synthetic medium suggested by the authors earlier was useful for the growth and toxin formation of cl. tetani, cl. botulinum and cl. perfringens, types b and e. a study of the character of growth and toxinogensis, microscopic examination of morphology of culture cells and results of passages showed the suggested synthetic medium to be of value; a possibility of its application for studying the nutrient requirements and the role of individual components of the nutrient media i ... | 1975 | 164749 |
| ulcerative enteritis--clostridial antigens. | the causative agent of ulcerative enteritis (ue) is a clostridium and it is believed that it belongs to a new, hitherto undescribed species. it has been named, tentatively, clostridium colinum. transmission experiments carried out with artificial cultures of this clostridium have clarified some aspects of the pathogenesis of ue and have brought into focus other aspects of its pathogenesis, epizootiology, and immunology that need to be investigated. support for research is needed: 1) to investiga ... | 1975 | 164804 |
| comments on ulcerative enteritis. | 1975 | 164805 | |
| lipid-protein interactions in mitochondria. the effect of protein interaction on susceptibility of phospholipids to phospholipase a2 and c hydrolysis. | 1975 | 164836 | |
| studies on interaction between histone v (f2c) and deoxyribonucleic acids. | histone v (2fc) from chick erythroctes was used in the study of its interaction with dna from various sources. complexes between this histone and dna were formed using the procedure of continuous nacl gradient dialysis in urea. two physical methods, namely thermal denaturation and circular dichroism (cd), were used as analytical tools. thermal denaturation of nucleohistone v with chick or calf thymus dna shows three melting bands: band i at 45-50 degrees corresponds to free base pairs; band ii a ... | 1975 | 164887 |
| structures of gangliosides from bovine adrenal medulla. | five gangliosides, accounting for over 95% of the total ganglioside fraction, were isolated from bovine adrenal medulla by preparative thin-layer chromatography and the carbohydrate structures determined by a combination of periodate oxidation and permethylation techniques. partially methylated alditol acetates were generated from the neutral sugars of the fully methylated glycolipids and identified by gas-liquid chromatography. substitution on n-acetylgalactosamine was determined by methanolysi ... | 1975 | 164890 |
| interaction of clostridium perfringens theta-haemolysin, a contaminant of commercial phospholipase c, with erythrocyte ghost membranes and lipid dispersions. a morphological study. | commercially available preparations of phospholipase c from clostridium perfringens are commonly contaminated with theta haemolysin, one of a group of bacterial haemolysins called oxygen labile (o-labile) haemolysins. treatment of erythrocyte ghosts and a mixed lipid dispersion containing cholesterol with commercially available phospholipase c in the absence of ca-2+ and the presence of phosphate buffer and/or edta resulted in the formation and release of ring or arc-shaped structures. highly pu ... | 1975 | 164911 |
| sialoglycopeptides from bovine milk fat globule membrane. | milk fat globule membrane was shown to contain sialic acid, all of which could be released without disruption of the fat globule. sialoglycopeptides were cleaved from the surface of intact fat globules by pronase and fractionated on sephadex g-50. further fractionation of the major sialoglycopeptide peak on deae-sephadex gave two groups of sialoglycopeptides eluted with 0.1 m nacl (group a) and 0.5 m nacl (group b), respectively. refractionation gave a major sialoglycopeptide from each of the tw ... | 1975 | 164945 |
| clostridium welchii corneal ulcer--a case report. | a case of corneal ulcer in a 10-year-old nigerian boy in which clostridium welchii and achromobacter were isolated is reported. the absence of trauma and rarity of only corneal involvement in a clostridial infection is emphasized. the bacteriology of the clostridial infection is emphasized. the bacteriology of the clostridial ocular infections is reviewed, the media necessary for isolation of the organism and the symbiotic relationship with other bacteria are discussed. the clinical course of th ... | 1975 | 164996 |
| restorative effect of normal human serum, transfer factor and thymosin on the ability of heated human lymphocytes to form rosettes with sheep erythrocytes. | 1975 | 165007 | |
| changes in surface carbohydrate of human erythrocytes aged in vivo. | 1975 | 165104 | |
| the mucosubstance coating the pneumonocytes in the lungs of xenopus laevis and lacerta viridis. | the layer of mucosubstance that is associated with the free surface membranes of the pneumonocytes in the lungs of the toad xenopus laevis and the lizard lacerta viridis was demonstrated by electron microscopy using iron oxide stain. the form and staining reactions of the mucosubstance layer were similar in both animals. in electron micrographs the mucosubstance was represented by a band of densely stained material (25-50 nm thick) which coated the entire free surface of the pneumonocytes. it ap ... | 1975 | 165163 |
| tritium labeling of sialylated glycoprotein hormones. | 1975 | 165378 | |
| interconversion of bacterial agents causing botulism and gas gangrene. | 1975 | 165441 | |
| the effect of phospholipase c treatment on the latency and sedimentability of rat liver microsomal nucleoside diphosphatase. | 1975 | 165963 | |
| the physiological function of nitrate reduction in clostridium perfringens. | fermentation-balance studies have been carried out on clostridium perfringens grown in the presence and absence of nitrate in the medium. nitrate is able to serve as an electron acceptor for these bacteria, permitting increased growth yields over those obtained in its absence. this increase is due to an increase in the proportion of metabolite molecules which can participate in substrate-level phosphorylation reactions when an inorganic acceptor is available. the nitrate reduction can be regarde ... | 1975 | 166143 |
| [acute bovine mastitis caused by clostridium perfringens type a (author's transl.)]. | an acute case of bovine mastitis caused by clostridium perfringens type a is described. the condition appeared before delivery and was characterised by gas formation in the udder and severe general signs. in spite of parenteral treatment with large doses of penicillin and streptomycin combined with frequent stripping of the udder the animal died two days after the onset of symptoms. the strain isolated proved sensitive to penicillin, chloramphenicol and tetracyclin, but resistant to streptomycin ... | 1975 | 166353 |
| [effectiveness of gas gangrene anatoxins in revaccination by the aerosol method]. | 1975 | 166512 | |
| acute enteritis in rabbits: spontaneous infection by clostridium perfringens type a. | 1975 | 166529 | |
| [etiology of anaerobic infections in cattle and sheep in latin america. i. field experience and laboratory research]. | 1975 | 166532 | |
| the effect of phospholipase c treatment of microsomes on cytochromes p-450 and b5. | 1975 | 166883 | |
| interactions between aerolysin, erythrocytes, and erythrocyte membranes. | aerolysin, a hemolytic and lethal exotoxin of aeromonas hydrophila, was analyzed for amino acids. assuming 8 histidine residues/mol, the purified toxic protein has, by summation, a molecular weight of 49,000, a value in agreement with earlier estimates by other methods. erythrocytes from different animal species differ greatly in sensitivity to aerolysin's lytic action. there is some correlation between sensitivity and phosphatidyl choline content. erythrocyte membranes of different species bind ... | 1975 | 166917 |
| clostridium welchii arthritis. case report. | 1975 | 167025 | |
| vascular leakage produced in the brains of mice by clostridium welchii type d toxin. | 1975 | 167060 | |
| the identification and purification of multiple forms of theta-haemolysin (theta-toxin) of clostridium perfringens type a. | the theta-haemolysin of clostridium perfringens was purified from culture supernatant fluids of type a strains by fractional ammonium sulphate precipitation and isoelectric focusing in narrow ph 5 to 8 gradients. four components detected on electrofocusing were designated theta-1(pi6-8to6-9),theta-2(pi6-5to6-6),theta-3(pi6-1to6-3) and theta-4(pi5-7to5-9). specific activities ranged from 0-4 x 10-6 to 1-2 x 10-6 haemolytic units/mg protein and 2950 to 3600 ld-50/mg protein. each haemolytic compon ... | 1975 | 167102 |
| the production of neuraminidase by food poisoning strains of clostridium welchii (c. perfringens). | the production of neuraminidase by a classical strain of clostridium welchii (c. perfringens) type a was studied. good yields were produced in 5% proteose peptone-water medium (ppw5); the enzyme was essentially extracellular but some further neuraminidase could be released by ultrasonic disintegration of the cells. this also released n-acyl neuraminic acid-aldolase (nan-aldolase) and the degree to which this interferes with the assay for neuraminidase was evaluated. forty-one british reference f ... | 1975 | 167169 |
| buoyant density sedimentation of macromolecules in sodium iothalamate density gradients. | 1975 | 167175 | |
| phospholipase c catalyzed formation of sphinogomyelin--14c from lecithin and n-(-14c)-oleoyl-sphingosine. | commercial preparations of clostreidium perfringens were incubated with phosphatidyl choline and n-1-(-14c) oleoylsphingosine. a radioactive product was formed which cochromatogramed with spingomyelin standard in three different solvent systems. several other phospholipases and phosphatases were unable to catalyze this reaction. neither choline, phosphoryl choline, cytidine diphosphate choline nor p-nitrophenyl phosphoryl choline were acitve donors. sphingomyelin was only slightly active as ... | 1975 | 167259 |
| specifity of dna-basic polypeptide interactions. influence of neutral residues incorporated into polylysine and polyarginine. | an approach is described for evaluation of the specificity of basic polypeptides concerning the base pair composition of dna. the polypeptides were covalently bound to cnbr activated agarose and two dnas strongly different in base composition but of equal molecular weight were loaded and detached by a nacl gradient. the difference in the nacl concerntrations between the elution maxima of the two dnas was taken as a measure for the recognition specificity. the results obtained confirmed the known ... | 1975 | 167352 |
| antibiotic susceptibility testing of anaerobic bacteria by the standardized disc diffusion method with special reference to bacteroides fragilis. | the susceptibility of 90 strains of bacteroides fragilis to 16 antibacterial drugs was determined by the agar plate dilution test and the disc diffusion test. good correlation of results was obtained with the two methods, and regression line analysis could be performed for beta-lactam antibiotics, erythromycin, tetracyclines, lincomycin and clindamycin. thus the standardized disc diffusion method can be used for susceptibility testing, but only a clear distinction between sensitive and resistant ... | 1975 | 167431 |
| outbursting wedges of the colonies of clostridium welchii containing chain forming mutants after ultra violet irradiation. | cl. welchii nctc 6785 was irradiated with uv light. the 40 minutes irradiated cells after 3-8 days ageing at room temperature (22-25 degrees c) and 48 hours initial incubation at 37 degrees c produced hairy outbursting wedges. these wedges gave rise to the colonies composed of chain forming mutants, which on transfer into the fluid medium grew into a pure chain forming culture. | 1975 | 167536 |
| anaerobic infections of the female genital tract. | anaerobic bacterial organisms are a frequent cause of female genital tract infections. there has been a lack of appreciation of the significant role anaerobes play in obstetric and gynecologic infections. utilization of newer appropriate anaerobic culture techniques has shown the importance of these organisms as pathogens in serious infections of the genital tract. many species of anaerobic bacteria appear to be part of the normal microflora of the lower genital tract in female subjects. similar ... | 1975 | 167582 |
| a highly efficient solid-phase radioimmune assay for a clostridial toxin. | 1975 | 167605 | |
| growth curves of anaerobic bacteria in solid media. | simple pour plate and spectrophotometric techniques for the evaluation of growth curves of several anaerobic bacteria on solid media are described. three basic patterns of anaerobic growth were observed. the curves obtained were very reproducible when studied on separate occasions. the curves obtained by spectrophotometric measurement were comparable to those obtained by the pour plate method, especially when a large bacterial inoculum was used. limitations in the interpretation of the results a ... | 1975 | 167660 |
| wound infection during the yom kippur war: observations concerning antibiotic prophylaxis and therapy. | eighty-eight episodes of wound associated infection were identified among 624 consecutively admitted battlefield casualties. ninety-one per cent of infections occurred during the administration of antibiotic therapy or prophylaxis and 65% were associated with the use of multiple antibacterial agents. gram negative bacillary and mixed microbial infection predominated and were found to increase in relative incidence after the second day of hospitalization. appropriate therapy, based on disc sensit ... | 1975 | 167666 |
| on the structure of the peptidoglycan of cell walls from myxobacter al-1 (myxobacterales). | basically the peptidoglycan of myxobater al-1 consists of alternating beta-1,4-linked n-acetylglucosamic-n-acetylmuramic acid chains. after splitting the aminosugar backbone with a specific algal enzyme three subunits arise: a monomer, a dimer and a timer. investigation of the monomer with specific enzymes and comparison of the degradation products to standards derived from other bacterial peptidoglycans suggest the following structure of the monomer peptide: l-alanyl-d-glutamic-l-meso-diaminopi ... | 1975 | 167671 |
| presence of endo-beta-n-acetylglucosaminidase and protease activities in the commercial neuraminidase preparations isolated from clostridium perfringens. | 1975 | 167779 | |
| template specificity of the cytoplasmic dna polymerase in xenopus laevis oocytes. | 1975 | 167780 | |
| the dna melting transition in aqueous magnesium salt solutions. | the melting transition of the magnesium salt of dna has been systematically examined in the presence of various types of anions. the addition of clo4- to a concentration of 3.0 n results in the biphasic optical transition, with the first phase exhibiting rapid reversibility and independence of the dna concentration. this subtransition, which is interpreted as an intramolecular condensation to a collapsed form of dna, is followed by a dna concentration-dependent aggregation reaction. the aggregat ... | 1975 | 167826 |
| characterization of human alpha-galactosidase a and b before and after neuraminidase treatment. | it has been previously reported that following neuraminidase treatment alpha-galactosidase a is converted into the b form, as revealed by electrophoresis. by a variety of techniques such as isoelectrofocusing, deae-chromatography and by enzyme kinetic parameters, no conversion of alpha-galactosidase a into b, or the reverse, could be detected after neuraminidase treatment. only an apparent transformation of alpha-galactosidase a into b was revealed by cellogel electrophoresis. in addition, a dis ... | 1975 | 167833 |
| [plasmids controlling the resistance to several antibiotics in c. perfringens type a, strain 659]. | a strain of c. perfringens type a, isolated from a patient, was found to be resistant to four antibiotics: tetracycline (tet), chloramphénicol (chl), erythromycin (ero) and clindamycin (cli). clones resistant to only two drugs (tet-chl or ero-cli), or sensitive to all drugs were found in cultures of the wild-type strain treated by acridine dyes or ethidium bromide. dna analysis by equilibrium centrifugation confirmed that the original strain contains two resistance plasmids, one called rip 401 ( ... | 1975 | 167997 |
| interactions of heteroaromatic compounds with nucleic acids. 2. influence of substituents on the base and sequence specificity of intercalating ligands. | this paper presents the results of a systematic study on the effects of substituents on the base and sequence specificity of tricyclic heteroaromatic compounds interacting with dna by intercalation. all the compounds tested are derived from proflavine and acridine orange analogs with different heteroatoms in the middle ring. their base and sequence specificities were determined by differential dialysis of the ligand against dna samples of differing g-c content. the main results indicate that (a) ... | 1975 | 168079 |
| effects of clostridium perfringens enterotoxin on metabolic indexes of everted rat ileal sacs. | everted sacs of rat ileum were incubated in ringer phosphate solution while oxygen uptake, glucose uptake, and lactate production were determined. sacs treated with clostridium perfringens enterotoxin, in the form of crude cell-free extract and purified protein, consumed significantly less oxygen than untreated sacs. however, the toxin-treated sacs took up glucose and produced lactate at levels that were not significantly different than those observed in controls. we conclude that oxidative meta ... | 1975 | 168153 |
| measurement of biological activities of purified and crude enterotoxin of clostridium perfringens. | enterotoxin of clostridium perfringens was assayed and compared with toxicity in mice and erythemal activity in guinea pigs. conversion factors were used to express these biological activities of crude enterotoxin in terms of weight of pure enterotoxin protein. one microgram of enterotoxin was equivalent to 3.41 erythema units and to 0.68 mouse median lethal dose. | 1975 | 168155 |
| the quantitative bacteriology of some commercial bivalve shellfish entering british markets. | incidents of non-specific illness associated with the consumption of oysters have highlighted the lack of published information on the bacteriology of shellfish suitable for consumption. investigations showed that the majority of molluscan shellfish entering english markets conform to the accepted standard of less than 5 escherichia coli/ml. tissue. the numbers of e. coli were related to the sanitary quality of the growing area but no relation could be established between numbers of e. coli and ... | 1975 | 168251 |
| taxonomy of the clostridia: ribosomal ribonucleic acid homologies among the species. | rrna homologies have been determined on reference strains representing 56 species of clostridium. competition experiments using tritium-labelled 23s rrna were employed. the majority of the species had dna with 27 to 28% guanine plus cytosine (%gc). these fell into rrna homology groups i and ii, which were well defined, and a third group which consisted of species which did not belong in groups i and ii. species whose dna was 41 to 45% gc comprised a fourth group. thirty species were placed into ... | 1975 | 168308 |
| radiation resistance of spores of some clostridium perfringens strains. | clostridium perfringens spores (eight strains) were irradiated in a model system with 60co gamma rays at -30 c. the quantal response data obtained were analyzed with extreme value statistics. it was found (at the 95% confidence level) that all eight strains followed the same rate of death and that this rate was probably (at the 95% level) not exponential. the statistics did not exclude, however, a normal, lognormal, weibull, or related rate of spore kill. a more definitive study would be require ... | 1975 | 168810 |
| inhibition of glucose 6-phosphatase by pure and impure c-type phospholipases. reactivation by phospholipid dispersions and protection by serum albumin. | 1. pure or impure c-type phospholipases hydrolysed rat liver microsomal phosphatides in situ at 5 degrees or 37 degrees c. at 5 degrees c mean hydrolysis of total phospholipids was 90% by bacillus cereus and 75% by clostridium perfringens (clostridium welchii) c-type phospholipases. 2. four degrees of inhibition of glucose 6-phosphatase (d-glucose 6-phosphate phosphohydrolase; ec 3.1.3.9) resulted. (a) at 37 degrees c inhibition was virtually complete and apparently irreversible. (b) at 5 degree ... | 1975 | 168886 |
| [gas edema in broilers]. | 1975 | 169118 | |
| sensitive assay for detection of toxin-induced damage to the cytoplasmic membrane of human diploid fibroblasts. | a sensitive assay was developed for detection and quantitation of subtle permeability changes in the cytoplasmic membrane of human diploid fibroblasts. release of the non-metabolizable amino acid [1-14c]alpha-aminoisobutyric acid (aib; molecular weight (103) from the cytoplasm of prelabeled cells was used as an indicator of toxin-induced membrane damage. an optimal procedure for labeling these cells was designed after varying the conditions with regard to ph, temperature, concentration of aib, c ... | 1975 | 169201 |
| isolation and characterization of clostridium perfringens mutants altered in both hemagglutinin and sialidase production. | the relationship between the production of hemagglutinin and sialidase activities by clostridium perfringens was investigated by screening for mutants producing reduced levels of hemagglutinin activity. twelve mutants were isolated; all produced reduced levels of sialidase activity and several had other altered phenotypic markers. revertants that regained the ability to produce active hemagglutinin were isolated. all of these revertants produced increased sialidase activity. these results show t ... | 1975 | 169224 |
| the mechanism of action of cholera toxin in pigeon erythrocyte lysates. | the adenylate cyclase activity of intact pigeon erythrocytes begins to rise after about 20 min of exposure to cholera toxin. the maximum rate at which the cyclase activity increases appears to be limited by the number of toxin molecules which can reach an intracellular target. if the erythrocytes are made permeable to the toxin by a bacterial hemolysin, no such limit exists, and adenylate cyclase activity starts to rise immediately upon the addition of toxin, and continues to rise to a maximum a ... | 1975 | 169243 |