Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the origin of non-outbreak vibrio parahaemolyticus infections on guam. | a study of vibrio parahaemolyticus infected patients not associated with known outbreaks and controls matched for sex, ethnicity and age (+/- 5 years) was conducted on guam. cases were asked if they had eaten seafood within the 24-hour period preceding onset of illness and controls were asked if they had eaten seafood within the 24-hour period preceding their interview. cases were more likely than controls to have eaten seafood (or = 37.59, cl [8.30-220.24]). | 1994 | 8165739 |
| hemolymph lectins of the blue crab, callinectes sapidus, recognize selected serotypes of its pathogen vibrio parahaemolyticus. | 1994 | 8192339 | |
| seasonal variations in the occurrence of vibrio vulnificus along the dutch coast. | the seasonal variation in the occurrence of v. vulnificus in relation to water temperature and salinity was studied along the dutch coast. in two consecutive years v. vulnificus strains could be isolated in august when the water temperature was highest. the indole-positive strains isolated from north sea water samples were identical to most strains isolated from human disease and from the environment. however, strains isolated from four of five patients living in countries around the north sea w ... | 1994 | 8150002 |
| [a case of cardiogenic shock caused by vibrio parahaemolyticus]. | a case of a 53 year old healthy female complaining of diarrhea and abdominal pain after taking raw fish is presented. she immediately went into shock and unconsciousness. central venous pressure was 8 cmh2o and her ecg showed a first-degree av block and st-t changes in almost all leads. after mechanical ventilation and administration of dopamine, dobutamine, cefotiam, ciprofloxacin, she became alert and recovered from her critical condition. v. parahaemolyticus which produces thermostable direct ... | 1994 | 8138673 |
| isolation and sequence of an endochitinase-encoding gene from a cdna library of trichoderma harzianum. | there are no reports of gene sequences coding for extracellular chitinolytic enzymes from filamentous fungi, even though these enzymes are considered critical to the biological control of plant pathogenic fungi. the purpose of this paper was to report the isolation of a gene (then-42) encoding endochitinase (ech) from trichoderma harzianum strain p1, describe its sequence, and to determine whether it was related to genes coding for enzymes with similar functions from prokaryotic or other eukaryo ... | 1994 | 8125293 |
| effect of ionizing dose rate on the radioresistance of some food pathogenic bacteria. | food pathogenic bacteria including listeria monocytogenes (1a1 and atcc 19111), staphylococcus aureus (gd13 and atcc 13565), escherichia coli o157:h7 (atcc 35150), salmonella typhimurium, yersinia enterocolitica, vibrio parahaemolyticus, and campylobacter jejuni were exposed to various rates of ionizing radiation (0.78, 2.6, and 22 kgy/h) emitted by three different 60co irradiators. d10 values (d10 is the radiation dose required to eliminate 90% of a bacterial population (one logarithmic cycle r ... | 1994 | 8069779 |
| responses of diverse heterotrophic bacteria to elevated copper concentrations. | the influence of copper on the growth of escherichia coli, pseudomonas aeruginosa, bacillus cereus, bacillus subtilis, vibrio parahaemolyticus, vibrio alginolyticus (three strains), and an unidentified vibrio sp. was examined in batch cultures. the effects of copper at micromolar concentrations varied from undetectable to complete growth inhibition. each strain was able to recover from a growth lag observed after copper addition at a characteristic concentration. copper concentrations that allow ... | 1994 | 8069784 |
| urea hydrolysis can predict the potential pathogenicity of vibrio parahaemolyticus strains isolated in the pacific northwest. | the ability of some strains of vibrio parahaemolyticus to hydrolyze urea (uh+) can be used as a marker to predict which strains isolated from molluscan shellfish harvested in the pacific northwest are potentially pathogenic. the thermostable direct hemolysin-producing (tdh+) characteristic is a marker that is correlated with potential pathogenicity, and all of the tdh+ strains that we have isolated have been found to be uh+. most of the uh+ strains belong to somatic antigen groups o3, o4 and o5. ... | 1994 | 8085837 |
| moty, a component of the sodium-type flagellar motor. | energy to power the rotation of bacterial flagella can be derived from the proton or sodium transmembrane potential. until now, genes encoding a bacterial sodium-type flagellar motor have not been defined. a gene, moty, encoding one component of the sodium-type flagellar motor of vibrio parahaemolyticus was cloned by complementation of a mot- mutant strain. sequencing revealed an open reading frame of 879 nucleotides in which a transposon conferring a motility defect mapped. overexpression of mo ... | 1994 | 8021208 |
| a mutant toxin of vibrio parahaemolyticus thermostable direct hemolysin which has lost hemolytic activity but retains ability to bind to erythrocytes. | a mutant toxin, r7, of thermostable direct hemolysin (tdh) with a single amino acid substitution at glycine 62 was analyzed. the hemolytic activity of r7 decreased to less than 1/1,000 of that of wild-type tdh, and its mouse lethality was undetectable. this mutant toxin, however, showed a marked inhibitory effect on hemolysis by wild-type tdh. enzyme immunoassay and flow cytometric analysis demonstrated that r7 retained approximately 50% of the ability to bind to erythrocytes compared with that ... | 1994 | 8039901 |
| construction and characterization of an isogenic mutant of vibrio parahaemolyticus having a deletion in the thermostable direct hemolysin-related hemolysin gene (trh) | a mutant, vp-mx02, having a deletion in the gene (trh) for the thermostable direct hemolysin-related hemolysin (trh) was constructed by double-crossover gene conversion from a trh-producing vibrio parahaemolyticus strain, th3996. the deleted region was the upstream half of trh. hemolysis was completely lost, and trh antigen was undetectable in the mutant. administration of the mutant into ligated rabbit small intestines elicited partial, but apparent, fluid accumulation. these results suggest th ... | 1994 | 8045908 |
| correlation between cell-associated mannose-sensitive hemagglutination by vibrio parahaemolyticus and adherence to a human colonic cell line caco-2. | cell-associated hemagglutination (cha) activity with human erythrocytes was examined for 468 clinical and 71 environmental strains of vibrio parahaemolyticus. approximately 95% of the strains tested were cha positive irrespective of source or kanagawa phenomenon. 75% of clinical strains showed relatively strong mannose-sensitive hemagglutination (msha), whereas 88% of the environmental strains showed relatively weak mannose-resistant hemagglutination (mrha). adherence of v. parahaemolyticus to c ... | 1994 | 7914500 |
| [the ecological and epidemiological aspects of the spread of vibrio parahaemolyticus in a freshwater region]. | for the first time vibrio parahaemolyticus have been isolated in this country in a freshwater region both from patients with the diarrheal syndrome and from environmental objects. in the region of the lower course of the volga morbidity in acute enteric infections (aei) caused by these vibrios may reach 9.1% of all aei cases in some years. the formation of an ecological niche suitable for the circulation of these bacteria has taken place in the volga delta. all these facts indicate that freshwat ... | 1994 | 7941868 |
| characterization of a glucose transport system in vibrio parahaemolyticus. | cells of a glucose-pts (phosphoenolpyruvate:carbohydrate phosphotransferase system)-negative mutant of vibrio parahaemolyticus transport d-glucose in the presence of na+. maximum stimulation of d-glucose transport was observed at 40 mm nacl, and na+ could be replaced partially with li+. addition of d-glucose to the cell suspension under anaerobic conditions elicited na+ uptake. thus, we conclude that glucose is transported by a na+/glucose symport mechanism. calculated vmax and km values for the ... | 1994 | 7961512 |
| [serotypes of urease producing vibrio parahaemolyticus and their relation to possession of tdh and trh genes]. | we analysed 467 isolates of vibrio parahaemolyticus for possession of tdh/trh gene in comparison with urease production and serotypes. strains possessing tdh+/trh-, tdh+/trh+, tdh-/trh-, and tdh-/trh- show positive urease production 2.1, 100, 65.7, 100%, respectively. serotypes of o1:k69, o3:k6, o3:k72, o6:k18, o6:k46 and o1:kut were frequently positive (100% except 91.7% of the latest one) in urease production. all isolates of o3:k6 prossessed trh, whereas all isolates of certain serotypes incl ... | 1994 | 7963797 |
| sleep-related periodic headache and imipramine. | 1994 | 197039 | |
| [priapism]. | 1994 | 302959 | |
| mechanism of quenching of fluorescein by anti-fluorescein igg antibodies. | 1994 | 303233 | |
| rem phase deprivation and schizophrenia ii. | nine schizophrenic patients with active symptomatology were compared with seven patient controls in their response to two nights of rapid eye movement (rem) sleep deprivation. the control subjects demonstrate "normal" increases in total rem and percentage rem time increase on recovery nights compared to base line nights. the schizophrenic subjects differ substantially from the control subjects in both these measurements and show no perceptible change from base line nights on recovery nights. the ... | 1995 | 173254 |
| influence of agents that act on dna and rna synthesis on the activity of poly(adp-rib) polymerase. | the activity of poly(adp-rib) polymerase is enhanced in the presence of spermine and spermidine. among the adenosine-like antibiotics tested, only formycin b and showdomycin cause an inhibition of the enzyme, which is competitive to nad. the activity of poly(adp-rib) polymerase is not reduced by rifamycin, alpha-amanitin and 2-phenylethanol. | 1995 | 170123 |
| [mental deficiency]. | 1995 | 1005559 | |
| isolation of mutant toxins of vibrio parahaemolyticus hemolysin by in vitro mutagenesis. | thermostable direct hemolysin produced by vibrio parahaemolyticus is a major virulence factor of the organism. the hemolysin has a variety of biological activities such as lethality to mice, cytotoxicity to cultured cells, cardiotoxicity, and fluid accumulating activity in rabbit ileal loop test. in this study, we attempted to isolate less hemolytic mutant toxins of the thermostable direct hemolysin to use them for analysis of mode of action of the hemolysin. six mutant toxins were obtained by i ... | 1995 | 7597724 |
| iron transport genes of the pjm1-mediated iron uptake system of vibrio anguillarum are included in a transposonlike structure. | the pjm1 genes encoding the proteins involved in iron transport in the anguibactin iron uptake system were found to be flanked by insertion sequences in a composite transposonlike structure. these vibrio anguillarum insertion sequences, isv-a1 and isv-a2, are related to is903, is102, and the isvs found in vibrio parahaemoliticus, vibrio mimicus, and non-o1 vibrio cholerae flanking various tdh (thermostable direct hemolysin) genes. the inverted repeats at the ends of isv-a1 and isv-a2 have no mor ... | 1995 | 7568465 |
| comparison of the fatty acids of the tunicate botryllus schlosseri from the black sea with two associated bacterial strains. | the fatty acid composition of the tunicate botryllus schlosseri and of two bacterial strains found within the tunicate, namely vibrio parahaemolyticus and of an associated but previously unreported gram positive cocci were studied. the polyunsaturated fatty acids 6,9,12-octadecatrienoic acid, 5,8,11,14,17-eicosapentaenoic acid, and 4,7,10,13,16,19-docosahexaenoic acid were particularly abundant in b. schlosseri and were not detected in the two bacterial strains found in the tunicate. the iso/ant ... | 1995 | 7564924 |
| purification and characterization of a cell-associated hemagglutinin of vibrio parahaemolyticus. | we found a positive correlation between cell-associated mannose-sensitive hemagglutination and adherence of vibrio parahaemolyticus to rabbit enterocytes by investigating 35 strains of v. parahaemolyticus for cell-associated hemagglutinin (cha) and for the ability to adhere to the enterocytes. we purified a mannose-sensitive cha from a kanagawa phenomenon-positive clinical strain of v. parahaemolyticus that exhibited a high level of mannose-sensitive hemagglutination and strongly adhered to the ... | 1995 | 7729912 |
| genetic and molecular characterization of the polar flagellum of vibrio parahaemolyticus. | vibrio parahaemolyticus possesses two alternate flagellar systems adapted for movement under different circumstances. a single polar flagellum propels the bacterium in liquid (swimming), while multiple lateral flagella move the bacterium over surfaces (swarming). energy to rotate the polar flagellum is derived from the sodium membrane potential, whereas lateral flagella are powered by the proton motive force. lateral flagella are arranged peritrichously, and the unsheathed filaments are polymeri ... | 1995 | 7883718 |
| variability of peptidoglycan structural parameters in gram-negative bacteria. | muropeptide composition of peptidoglycan from the gram-negative bacteria aeromonas sp., acinetobacter acetoaceticus, agrobacterium tumefaciens, enterobacter cloacae, proteus morganii, pseudomonas aeruginosa, pseudomonas putida, vibrio parahaemolyticus yersinia enterocolitica and escherichia coli, was analyzed by hplc. in all instances peptidoglycan was built up from the same subunits. a wide disparity in the relative abundance of muropeptides and all structural parameters was observed. the contr ... | 1995 | 7867925 |
| characterisation of a haemolysin related to vp-tdh produced by a kanagawa phenomenon-negative clinical isolate of vibrio parahaemolyticus. | the production of a family of haemolysins--thermostable direct haemolysin (vp-tdh), vp-tdh-related haemolysin (vp-trh) and vp-tdh/i--has been reported in clinical isolates of vibrio parahaemolyticus. this paper describes a fourth type of haemolysin--vp-tdh/ii--produced by a kanagawa phenomenon-negative clinical isolate of v. parahaemolyticus (o13:k, untypable). vp-tdh/ii was purified by ammonium sulphate precipitation and successive filtrations on deae-cellulose, hydroxyapatite, sepharose 4b and ... | 1995 | 7869352 |
| [choice of commercial elective nutrient media for the isolation of pathogenic vibrios of different species]. | commercial nutrient media and their modifications are assessed, including elective differentiation medium for v. cholerae developed at the rostov research anti-plague institute for the isolation of pathogenic vibrios. v. cholerae cholerae p-1 (145), v. cholerae el tor m-878, v. cholerae non 01 p-9741, e. coli 18, p. vulgaris 19, and 48 strains of vibrios belonging to different species were used in the study. all the strains used were characterized as to their nutritive requirements. alkaline aga ... | 1995 | 7670825 |
| [vibrios pathogenic to humans and laboratory diagnosis of diseases caused by them]. | present-day taxonomic status of vibrios pathogenic for humans is discussed, as are the phenotypical characteristics of some species, factors of virulence, and clinical manifestations of diseases caused by these agents. a scheme of isolation and identification of vibrios pathogenic for humans is offered. | 1995 | 8689064 |
| [the discovery of a dermonecrotic factor in vibrio cholerae non o1 and other pathogenic vibrios]. | 1995 | 7771160 | |
| utilization of hemin and hemoglobin as iron sources by vibrio parahaemolyticus and identification of an iron-repressible hemin-binding protein. | several clinical isolates of vibrio parahaemolyticus were examined for their ability to utilize either hemin or hemoglobin as a sole source of iron. both compounds appeared to be equally good iron sources. maximum growth was obtained at 5 microm hemin or 1.25 microm hemoglobin under the conditions tested. using a hemin-agarose batch affinity method, the hemin-binding protein was isolated from crude total membranes of a hemin-utilizing strain, wp1, grown under iron-deficient but not under iron-su ... | 1995 | 7750738 |
| ca(2+)-independent cytotoxicity of vibrio parahaemolyticus thermostable direct hemolysin (tdh) on intestine 407, a cell line derived from human embryonic intestine. | the effects of vibrio parahaemolyticus thermostable direct hemolysis on intestine 407, a cell line derived from the intestine of human embryos, were investigated. the hemolysin was shown to be cytotoxic to intestine 407. this cytotoxicity is accompanied by the damage of plasma membrane and lysosomes, as well as cellular degeneration in the form of large transparent blebs. although an increase in cytosolic free ca2+ due t the influx of extracellular ca2+ was observed in cells treated with thermos ... | 1995 | 8586273 |
| cloning and sequence analysis of vibrio parahaemolyticus ompk gene encoding a 26-kda outer membrane protein, ompk, that serves as receptor for a broad-host-range vibriophage, kvp40. | the ompk gene of vibrio parahaemolyticus 1010 (rimd 2210001) encoding an outer membrane protein (omp), ompk, which serves as the receptor for a broad-host-range vibriophage, kvp40, was cloned and sequenced. the gene consisted of 789 nucleotides encoding 263 amino acids. since the first 20 amino acids most likely constitute the signal peptide, mature ompk would consist of 243 amino acids with a calculated molecular mass of 27458 da. sequence comparisons indicate that ompk is unique among vibrio o ... | 1995 | 8586275 |
| thermostable direct hemolysin gene of vibrio parahaemolyticus: a virulence gene acquired by a marine bacterium. | 1995 | 7768586 | |
| tolerance to acid in ph 5.0-grown organisms of potentially pathogenic gram-negative bacteria. | a wide range of potentially pathogenic species of gram-negative bacteria were far more resistant to extreme acidity (ph 2.0-3.5) when cultured at ph 5.0 (habituated to acid) than after ph 7.0 culture. the differences were particularly great for citrobacter spp., enterobacter spp., klebsiella spp. and for vibrio parahaemolyticus; substantial habituation was also observed for proteus mirabilis and aeromonas formicans but the effect was less marked for serratia marcescens and acinetobacter calcoace ... | 1995 | 8554762 |
| [recent food poisoning and molecular biology]. | 1995 | 8568396 | |
| demonstration of a ferric vibrioferrin-binding protein in the outer membrane of vibrio parahaemolyticus. | under iron-restricted conditions, vibrio parahaemolyticus produces a siderophore, vibrioferrin, accompanying expression of two major outer membrane proteins of 78 and 83 kda. autoradiographic analysis of nondenaturing polyacrylamide gel electrophoregrams of outer membrane preparations previously incubated with [35fe]ferric vibrioferrin revealed a single radiolabeled band, in which the 78-kda protein was detected predominantly by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the anti ... | 1995 | 8577266 |
| evaluation of two assay kits for thermostable direct hemolysin (tdh) as an indicator of tdh-related hemolysin (trh) produced by vibrio parahaemolyticus. | reversed passive latex agglutination (rpla) or enzyme-linked immunosorbent assay kits with beads (bead-elisa) are commercially available in japan to detect the thermostable direct hemolysin (tdh) produced by vibrio parahaemolyticus isolates. we evaluated whether these kits can be used to assay the pathogenic toxin, tdh-related hemolysin (trh), produced by some so-called kanagawa phenomenon-negative v. parahaemolyticus strains isolated from patients with diarrhea. our results showed that the two ... | 1995 | 7783689 |
| a novel method to chemically immobilize antibody on nylon and its application to the rapid and differential detection of two vibrio parahaemolyticus toxins in a modified enzyme-linked immunosorbent assay. | a new method of chemically immobilizing antibody on nylon was developed. the method consists of serial treatments with hcl, polyethylene imine, and maleic anhydride methylvinyl ether copolymer, which resulted in the stable immobilization of sufficient amounts of antibodies on nylon. this principle was used to differentially detect two immunologically related but nonidentical hemolysins (thermostable direct hemolysin [tdh] and tdh-related hemolysin [trh]) of vibrio parahaemolyticus in a modified ... | 1995 | 7697525 |
| a 26-kda outer membrane protein, ompk, common to vibrio species is the receptor for a broad-host-range vibriophage, kvp40. | kvp40 is a broad-host-range vibriophage forming plaques on strains of at least eight vibrio and one photobacterium species. a spontaneous kvp40-resistant mutant, r4000, derived from vibrio parahaemolyticus 1010 lacked a 26-kda outer membrane protein designated ompk. kvp40 was inactivated by outer membrane and ompk prepared from 1010, but not by outer membrane from r4000. these results strongly suggest that ompk is the receptor for kvp40. immunoblotting analyses using an anti-ompk rabbit serum re ... | 1995 | 7867914 |
| [the destructive activity of vibrios]. | 1995 | 7771159 | |
| outbreak of diarrhea due to vibrio parahaemolyticus among military personnel in lima, peru. | 1995 | 8749651 | |
| effect of iron on the recovery of viable bacteria from the cardiac blood in an experimental mouse model and on the serum resistance and macrophage phagocytosis of vibrio parahaemolyticus. | the role of iron in the pathogenesis of vibrio parahaemolyticus, an intestinal pathogen, is not clearly understood. in an intraperitoneally infected mouse model, viable bacteria of a kanagawa-negative food isolate ccrc12958 and a kanagawa-positive clinical isolate st550 of v. parahaemolyticus were rapidly recovered from the cardiac blood before the death of these animals. iron-limitation during the preparation of bacterial culture decreased the appearance of ccrc12958 strain in the cardiac blood ... | 1995 | 8838823 |
| fatal vibrio parahemolyticus septicemia in a patient with cirrhosis. a case report and review of the literature. | vibrio parahemolyticus has been well documented to cause outbreaks of infectious diarrhea, usually related to poor food handling; only rarely has it been reported to cause fetal septicemia. in contrast, vibrio vulnificus is a well-known cause of septicemia, especially in patients with cirrhosis. a 31-year-old woman with cirrhosis who developed fatal v. parahemolyticus sepsis after ingesting raw seafood is described. we review the clinical syndromes associated with sepsis caused by these two orga ... | 1995 | 7781443 |
| a transferable multiple drug resistance plasmid from vibrio cholerae o1. | ten multiple antimicrobial-resistant isolates of vibrio cholerae o1 isolated from patients in uganda were characterized, and the transferability of resistance to bacteria of diverse origins was investigated. the isolates were toxigenic and belonged to biotype e1 tor, serotype ogawa, and ribotype 8, and possessed a 130-mda plasmid of incompatibility group 6-c. this plasmid, designated prvc1, was shown to confer resistance to trimethoprim (mediated by a dhfri gene), sulfonamides (a suii gene), tet ... | 1995 | 9158776 |
| characterization of the manganese-resistant mutants derived from vibrio parahaemolyticus. | the virulence and some related factors of vibrio parahaemolyticus are regulated by the level of iron. in this study, five mn-resistant mutants were selected after n-methyl-n'-nitrosoguanidine treatment and two transfers in medium containing high levels of manganese chloride. production of siderophores and the 77-kda iron-regulated outer-membrane protein and the bacterial growth in these mn-resistants were deregulated, as compared with the wild-type strain. in addition, the regulation of these ph ... | 1995 | 9775003 |
| role of outer membrane proteins on the adherence of vibrio parahaemolyticus to rabbit intestinal epithelial cell in vitro. | antiserum against outer membrane preparation of a kanagawa phenomenon-positive strain of vibrio parahaemolyticus were raised in rabbits and absorbed with their lipopolysaccharide. the anti-outer membrane protein serum and its fab (igg) fragment inhibited the adherence of kanagawa-positive strains to rabbit intestinal epithelial cells in vitro. preincubation of rabbit intestinal epithelial cells with outer membrane preparation also inhibited the adherence of these bacteria. anti-lipopolysaccharid ... | 1995 | 9810668 |
| effect of chill and freezing temperatures on survival of vibrio parahaemolyticus inoculated in homogenates of oyster meat. | survival of vibrio parahaemolyticus was determined in oyster meat homogenates at various temperatures. (4 degrees c, 0 degrees c, -18 degrees c and -24 degrees c) and bacterial levels (10(2), 10(4), 10(5) and 10(7) ml-1). in all cases, the numbers of v. parahaemolyticus were a logarithmic function of log time. this study indicates that high numbers of v. parahaemolyticus can be inactivated at low temperatures. the time of total inactivation depends on the initial number of micro-organisms and in ... | 1995 | 7766116 |
| sequence of a cloned pr72h fragment and its use for detection of vibrio parahaemolyticus in shellfish with the pcr. | the nucleotide sequence of pr72h cloned from vibrio parahaemolyticus 93 was determined. we examined all v. parahaemolyticus gene sequences published in the genbank-embl databases for homology and found that no other dna sequence of v. parahaemolyticus was highly homologous to the sequence reported in this study. a pair of primers, vp33-vp32, derived from a pr72h fragment were selected to detect v. parahaemolyticus. the sensitivity of pcr detection for a pure culture of v. parahaemolyticus was 10 ... | 1995 | 7747952 |
| shared amino acid sequences between major histocompatibility complex class ii glycoproteins, type xi collagen and proteus mirabilis in rheumatoid arthritis. | to show molecular similarity between two sequences of proteus mirabilis (haemolysin--esrral; urease--irret) with hla-dr antigens (eqrraa) which are associated with rheumatoid arthritis (ra) and type xi collagen (lrrei), respectively; and, in patients with ra, to measure levels of antibody against a 16-mer synthetic peptide containing the esrral sequence, and the haemolysin and urease proteins of proteus mirabilis. | 1995 | 7748019 |
| [phages of halophilic vibrios]. | the morphology and antigenic properties of 34 phages of vibrio parahaemolyticus and v. alginolyticus strains isolated from black sea water, as well as their range of lytic activity and specificity of action, have been studied. most of these phages have been found to lyze both v. parahaemolyticus and v. alginolyticus strains. in addition, highly specific phages capable of lyzing only v. parahaemolyticus serotype o5:k15, have been detected. the latter fact is a precedent indicating the possibility ... | 1995 | 7778366 |
| sequence alignment and structural modelling of the lamb glycoporin family. | lamb gene segments were obtained from yersinia enterocolitica and vibrio parahaemolyticus by the pcr and the dna sequence determined. the deduced polypeptide sequences showed high similarity to six other lamb-related proteins and all contained typical signature sequences present in all members of the family but not other proteins. the aligned amino acid sequences permitted derivation of a model of lamb folding across the bacterial outer membrane using an approach successfully applied in the iden ... | 1995 | 7702622 |
| identification of a 29 kda flagellar sheath protein in helicobacter pylori using a murine monoclonal antibody. | the membrane-like flagellar sheath of helicobacter pylori is of unknown function and little is known of its composition. a murine monoclonal antibody to h. pylori, designated gf6, which reacts by immunoblot with a polypeptide with an apparent molecular mass of 29 kda was shown by immunogold-electron microscopy to label specifically the flagellar sheath structure. the antigen was detected by immunoblot using the monoclonal antibody in all 11 strains, of diverse geographic origin, so far tested. t ... | 1995 | 7711897 |
| molecular analysis of an extracellular protease gene from vibrio parahaemolyticus. | the structural gene prtvp encoding the extracellular protease of vibrio parahaemolyticus strain 93 was cloned in escherichia coli and sequenced. the cloned dna fragment contained a 1761 bp orf encoding a 587 amino acid protein. the deduced polypeptide is composed of a 25 amino acid signal peptide and a 562 amino acid extracellular polypeptide with a calculated molecular mass of 63,156 da. protease analysis using a gelatin-containing sds-polyacrylamide gel detected the presence of a 62 kda protea ... | 1995 | 7582017 |
| urease production correlates with possession of the trh gene in vibrio parahaemolyticus strains isolated in thailand. | a total of 489 vibrio parahaemolyticus isolates from patients in thailand with diarrhea was examined for the presence of thermostable direct hemolysin (tdh) and tdh-related hemolysin genes (tdh and trh, respectively), their serovars, tdh production, and urease activity. of the strains, 81% were positive only for the tdh gene, 6% for both trh and tdh genes, and 2% for the trh gene only. thirty-seven (8%) of the 489 isolates were positive for urease production. of special interest, all urease-posi ... | 1995 | 7594689 |
| a simple and rapid method for transformation of vibrio species by electroporation. | 1995 | 7550730 | |
| distribution of the cytolethal distending toxin a gene (cdta) among species of shigella and vibrio, and cloning and sequencing of the cdt gene from shigella dysenteriae. | we investigated the distribution of the cytolethal distending toxin a gene (cdta) among s. dysenteriae, vibrio cholerae 01 and vibrio parahaemolyticus by polymerase chain reaction (pcr) using primers constructed from the nucleotide sequences of escherichia coli cdta gene reported independently by scott and kaper (infect immun 1994; 62: 244-51) and by pickett et al. (infect immun 1994; 62: 1046-51). the cdta gene reported by scott and kaper was found to occur among eight of the 35 strains of s. d ... | 1995 | 7565011 |
| characterization of a cloned pr72h probe for vibrio parahaemolyticus detection and development of a nonisotopic colony hybridization assay. | vibrio parahaemolyticus is a halophilic bacterium often found in shellfish and is an important causative agent of food poisoning in taiwan. a rapid and efficient detection method is required to identify this foodborne pathogen. a 0.76-kb hindiii dna fragment was cloned from the chromosomal dna of v. parahaemolyticus strain no. 93, designated as pr72h fragment, was used as a polynucleotide probe. it was labeled with digoxigenin-11-dutp (dig) by the random primer-labeling method. the sensitivity a ... | 1995 | 7603362 |
| nutrient starvation induces cross protection against heat, osmotic, or h2o2 challenge in vibrio parahaemolyticus. | glucose-starved cells of vibrio parahaemolyticus were compared with non-starved counterparts with respects to heat, osmotic, and oxidative challenges. the starved cells demonstrated greater thermal and oxidative resistance than did the non-starved cells. the starved cells also showed greater resistance against low osmotic challenge than did the non-starved cells although both cells showed a comparable resistance against high osmotic challenge. | 1995 | 7603366 |
| calcium-dependent intestinal chloride secretion by vibrio parahaemolyticus thermostable direct hemolysin in a rabbit model. | vibrio parahaemolyticus is a major agent of seafood gastroenteritis that induces intestinal secretion in the rabbit through its thermostable direct hemolysin. the aim of this study was to characterize the enterotoxicity of purified hemolysin in vitro. | 1995 | 7615186 |
| [correlation between trh possession and urease production of clinical isolates of vibrio parahaemolyticus]. | 1995 | 7616022 | |
| vibrio parahaemolyticus o serotypes from o1 to o13 all produce r-type lipopolysaccharide: sds-page and compositional sugar analysis. | the molecular architecture of lipopolysaccharide (lps) isolated from all o serotypes of vibrio parahaemolyticus was investigated. in gel chromatography on a sephadex g-50 column, the degraded polysaccharide fraction prepared from each serotype lps by mild acid hydrolysis yielded only core oligosaccharide (frc ii) and monosaccharide (frc iii) fractions, but no fraction (frc i) corresponding to o polysaccharide chain consisting of polymeric repeating oligosaccharide units. compositional sugar anal ... | 1995 | 7649452 |
| the binding of vibrio parahaemolyticus 125i-labeled thermostable directhemolysin to erythrocytes. | thermostable direct hemolysin (tdh) produced by vibrio parahaemolyticus was iodinated using chloramine t. the 125i-labeled tdh retained up to 80% of the activity of intact toxin. the binding of 125i-tdh to rabbit erythrocytes was inhibited by addition of nonlabeled tdh. the binding of 125i-tdh to rabbit erythrocytes was completed in the 1st or 2nd min of incubation at 37 degrees c in contrast to that at 4 degrees c. 125i-tdh, which cannot lyse horse erythrocytes as does intact tdh, bound to hors ... | 1995 | 7660369 |
| [a study of the cause of massive mortality among marine-cultured rainbow trout (oncorrhynchus mykiss, walbaum) in a fish farm in the southwestern netherlands]. | the present study investigated the mass mortality of marine cultured young rainbow trout (oncorhynchus mykiss, walbaum) in the netherlands. the course of the disease, the clinical symptoms, and bacteriological and virological investigations lead to the diagnosis: 'primary infectious pancreatic necrosis virus (ipnv) infection followed by secondary vibriosis'. treatment with flumequine seemed to be effective. the trout were possible infected with ipnv at the trout hatchery and nursery from which t ... | 1995 | 7482471 |
| properties of a na+/galactose (glucose) symport system in vibrio parahaemolyticus. | we have investigated galactose transport in a mutant strain of vibrio parahaemolyticus that lacks a glucose-pts (phosphoenolpyruvate:carbohydrate phosphotransferase system) and a trehalose-pts. cells of the v. parahaemolyticus actively transported d-galactose and na+ greatly stimulated the transport. maximum stimulation of d-galactose transport activity was observed at 10mm nacl, and na+ could be replaced with li+. addition of galactose to the cell suspension under anaerobic conditions elicited ... | 1996 | 8603081 |
| occurrence of urease-positive vibrio parahaemolyticus in kanagawa, japan, with specific reference to presence of thermostable direct hemolysin (tdh) and the tdh-related-hemolysin genes. | a total of 132 strains of v. parahaemolyticus isolated from patients and from the suspected causal food items of past food poisoning cases occurring in kanagawa prefecture, japan, were examined for the ability to hydrolyze urea, with specific reference to the presence of the thermostable direct hemolysin gene (tdh) and the gene for thermostable direct hemolysin-related hemolysin (trh). ten strains belonging to five different o-antigen serotypes were positive for urea hydrolysis (uh+), and four o ... | 1996 | 8593076 |
| study of the haemolytic process and receptors of thermostable direct haemolysin from vibrio parahaemolyticus. | the haemolytic action of 125i-labelled thermostable direct haemolysin from vibrio parahaemolyticus was studied on human and equine erythrocytes. in the first step, the haemolysin bound to the membranes of both erythrocyte species. this binding seemed temperature-independent. then, for human erythrocytes, haemolysin produced cell disruption, and haemoglobin was released. following this step, haemolysin was also released in a temperature-dependent manner. in contrast, equine erythrocytes were not ... | 1996 | 9296103 |
| phosphorylation of a 25 kda protein is induced by thermostable direct hemolysin of vibrio parahaemolyticus. | thermostable direct hemolysin (tdh) is a possible virulence factor produced by vibrio parahaemolyticus. although tdh has a variety of biological activities, including hemolytic activity, the biochemical mechanism of action remains uncertain. here we analysed biochemical events, especially phosphorylation, caused by tdh in erythrocytes, and found that tdh caused significant phosphorylations of proteins on erythrocyte membrane. phosphorylation of proteins was studies using [gamma-32p] atp and sds- ... | 1996 | 9022294 |
| hemolysins and plasmid profiles of vibrio parahaemolyticus. | forty clinical isolates of vibrio parahaemolyticus were studied for the production of the thermostable direct hemolysin (tdh), and the tdh-related hemolysin (trh) including the respective encoding genes, tdh and trh. the presence of tdh and its encoding genes were found amongst 95% of the strains, whereas the trh was absent amongst these isolates. thirty-two isolates were found to be plasmid-free, whereas eight isolates possessed plasmids with sizes ranging from 2.4 > or = 23 kb. using a dna pro ... | 1996 | 9031414 |
| isolation and partial characterization of polymyxin b-resistant isolates of vibrio parahaemolyticus. | two polymyxin-resistant isolates v2pxr and p1r4pxr of vibrio parahaemolyticus were spontaneously isolated and compared with the polymyxin-sensitive strains as to their chemical compositions of cell envelopes and their outer membrane protein compositions. when grown in the presence or absence of polymyxin, the cell envelopes of polymyxin-resistant isolates showed a significantly increased content of protein as compared with those of respective polymyxin-sensitive strains. both polymyxin-resistant ... | 1996 | 8857266 |
| vibrio parahaemolyticus flaj, a homologue of flis, is required for production of a flagellin. | the flaa locus of vibrio parahaemolyticus encodes one of the four polar flagellin genes, the flagellum-capping protein hap2, and three additional flagellar genes. sequence analysis downstream of the gene encoding hap2 revealed the region to be similar to the flid (hap2) locus of pseudomonas aeruginosa. the deduced protein sequences for the newly identified genes suggest that one protein belongs to the family of transcriptional regulatory proteins known to interact with sigma (54), one may be a r ... | 1996 | 8861212 |
| rapid polymerase chain reaction method for detection of kanagawa positive vibrio parahaemolyticus in seafoods. | detection of kanagawa positive strains of vibrio parahaemolyticus by polymerase chain reaction (pcr) was tested. primer pairs for specific amplification of tdh gene fragment is described. the assay could detect contamination of seafood homogenate when pcr was performed using lysate prepared directly from fish homogenates. the sensitivity of the assay could be improved to detect less than 10 cells of v. parahaemolyticus by performing pcr after 8 h enrichment in alkaline peptone water. | 1996 | 8880318 |
| [characteristics of energy transducing systems in membrane of vibrio parahaemolyticus]. | 1996 | 8840803 | |
| [origin of the pathogenic vibrios in the environment: inference from the studies on the molecular genetics of vibrio cholerae and vibrio parahaemolyticus]. | 1996 | 8840813 | |
| identification and characterization of additional flagellin genes from vibrio anguillarum. | previously, the flagellar filament of vibrio anguillarum was suggested to consist of flagellin a and three additional flagellin proteins, flab, -c, and -d. this study identifies the genes encoding flab, -c, and -d and a possible fifth flagellin gene that may encode flae. the flagellin genes map at two separate dna loci and are most similar to the four polar flagellin genes of vibrio parahaemolyticus, also located at two dna loci. the genetic organization of these two loci is conserved between bo ... | 1996 | 8752337 |
| isolation and characterization of restriction endonuclease in plesiomonas shigelloides and aeromonas species. | five restriction endonucleases (enases) and one enase were found in a screen of 196 strains of plesiomonas shigelloides and 147 strains of aeromonas species. plesiomonas and aeromonas species are classified as vibrionaceae, identified as food-poisoning bacteria, are closely genetically related to each other, and their enases producing abilities have not bee reported. enases were detected at relatively low frequencies in these species as compared to those in other species, such as salmonella spec ... | 1996 | 8951174 |
| detection of hsp60 (gro-el)-like proteins in vibrio parahaemolyticus and vibrio species by western immunoblotting analysis. | detection of heat shock proteins in vibrio parahaemolyticus was investigated by sds-page and western immunoblotting procedure using an anti-hsp 60 antibody. results indicate that v. parahaemolyticus elicited at least one hsp 60 (groel)-like protein with apparent molecular weight of about 58,000 (58 kda) when submitted to a heat shift from 30 to 42 degrees c. kanagawa phenomenon-positive and -negative strains of v. parahaemolyticus responded the same way. six other vibrio species also showed an i ... | 1996 | 8987708 |
| identification of pasteurella multocida tryptophan synthase beta-subunit by antisera against strain p1059. | pasteurella multocida strain p1059 is a highly virulent bacterium which causes fowl cholera in turkeys and chickens. a genomic library of p. multocida p1059 dna was constructed using puc19, expressed in escherichia coli dh5 alpha, and screened with chicken antisera generated against p. multocida p1059. twelve out of the 4100 clones screened were immunoreactive. plasmids isolated from these twelve clones were transformed into e. coli csr603 for maxicell analysis. five proteins, with molecular mas ... | 1996 | 8581158 |
| the epidemiology of vibrio infections in florida, 1981-1993. | the epidemiology of 690 vibrio infections reported in florida during 1981-1993 is described. most infections resulted in one of three clinical syndromes: gastroenteritis (51%), wound infections (24%), or primary septicemia (17%). case-fatality rates were 1% for gastroenteritis, 5% for wound infections, and 44% for primary septicemia. while gastroenteritis had little seasonal variation, 91% of primary septicemias and 86% of wound infections occurred from april through october, mostly due to the s ... | 1996 | 8627070 |
| cloning and sequencing of the gene for na+/h+ antiporter of vibrio parahaemolyticus. | a gene encoding an na+/h+ antiporter was cloned from vibrio parahaemolyticus into the plasmid pbr322 and expressed in escherichia coli cells. the gene enabled mutant e. coli cells to grow in the presence of 0.2 m nacl (or 10 mm licl). these cells were originally unable to grow under such conditions because of the lack of major na+(li+)/h+ antiporters. we detected na+/h+ antiporter activity due to the gene in membrane vesicles. the gene was sequenced and the deduced amino acid sequence was found ... | 1996 | 8651921 |
| sequence of a na+/glucose symporter gene and its flanking regions of vibrio parahaemolyticus. | the nucleotide sequence of an approximately 6 kbp segment of chromosomal dna of vibrio parahaemolyticus was determined. the nucleotide sequence revealed four open reading frames (orfs) in this region. hydropathy profiles of the deduced amino acid sequence of the orfs indicate that orf1 encodes a hydrophobic polypeptide with typical characteristics of a membrane transport protein. all other orfs encode hydrophilic polypeptides. orf1 showed significant amino acid sequence similarity to proteins of ... | 1996 | 8652595 |
| nature of the cation leak induced in erythrocyte membranes by kanagawa haemolysin of vibrio parahaemolyticus. | vibrio parahaemolyticus is an important enteric pathogen that produces an exotoxin prepared as kanagawa haemolysin (kh). isotope flux techniques were used to analyse toxin action on the basal permeability of human erythrocytes. kh induced a cation leak that was (i) rapid in onset (lag phase < 1 min), (ii) 'pore-like' in terms of kinetic characteristics, and (iii) of high magnitude initially (first 10 min) and then subsequently lower (but still raised with reference to control cells). the suscept ... | 1996 | 8664321 |
| food-borne disease outbreaks in taiwan, 1994. | in 1994, 102 outbreaks of food-borne disease involving 4,726 cases were reported to the taiwan department of health. this is the highest number of outbreaks and cases in recent years in taiwan. of these outbreaks, 72.5% (74/102) were caused by bacterial pathogens, with vibrio parahaemolyticus responsible for 56.7% (42/74), staphylococcus aureus 20.3% (15/74), bacillus cereus 14.9% (11/74) and salmonella spp other than s. typhi and s. paratyphi 8.1% (6/74). v. parahaemolyticus has been a leading ... | 1996 | 8688712 |
| comparison of a fluorogenic assay with a conventional method for rapid detection of vibrio parahaemolyticus in seafoods. | a conventional method and a fluorogenic assay for the detection of vibrio parahaemolyticus were compared. among 29 seafood samples examined for the presence of v. parahaemolyticus, 17 samples harbored v. parahaemolyticus, and trypsinlike activity was noticed in 19 seafoods. the added fluorogenic substrate was cleaved in single samples of shrimp, turbo, and cuttlefish from which v. parahaemolyticus could not be isolated by the conventional method. vibrio alginolyticus, in addition to v. parahaemo ... | 1996 | 8795246 |
| rapid detection of vibrio parahaemolyticus in oysters by immunofluorescence microscopy. | a method of indirect immunofluorescence microscopy was developed for the rapid detection of vibrio parahaemolyticus in oysters. affinity-purified antibodies against two outer membrane proteins (molecular mass 36 and 34 kda, respectively) of v. parahaemolyticus were used as the primary antibodies to label the vibrio, and fluorescein isothiocyanate-conjugated goat anti-rabbit immunoglobulin g was used to reveal the antigen-antibody reaction. of 85 strains of v. parahaemolyticus tested, all produce ... | 1996 | 8796431 |
| [bacteriological studies of travellar's diarrhoea. 5) analysis of enteropathogenic bacteria at osaka airport quarantine station from january 1992 through september 3rd, 1994]. | during the last 2 years and 8 months before the closure of osaka airport quarantine station (from jan. 1992 to sep. 3, 1994), a total of 7,421,909 overseas travellers were quarantined. 15,919 reported themselves of suffering from diarrhoea. bacteriological examination of a total of 6,031 individuals' stools were performed. 1) various enteropathogenic bacteria were isolated from 31.2% of the stools examined. isolated species were as follows: plesiomonas shigelloides, 1,127 cases (59.9%); vibrio p ... | 1996 | 8822051 |
| [in vitro antibacterial activity of pazufloxacin (pzfx) against clinical isolates from infectious enteritis]. | antibacterial activity of pazufloxacin (pzfx) against 65 strains of shigella spp., 13 strains of salmonella spp., 7 strains of escherichia coli, 4 strains of vibrio parahaemolyticus, 4 strains of v. cholerae o1, 2 strains of aeromonas spp., 4 strains of plesionomas shigelloides and 3 strains of v. cholerae non-o1 isolated from patients of infectious enteritis and carriers was compared with that of ofloxacin (oflx), ciprofloxacin (cpfx), tosufloxacin (tflx) and nalidixic acid (na). the mic90 of p ... | 1996 | 8822053 |
| production of thermostable direct hemolysin by vibrio parahaemolyticus enhanced by conjugated bile acids. | the effects of conjugated bile acids, glycocholic acid, and taurocholic acid (tc) on production of thermostable direct hemolysin (tdh) by vibrio parahaemolyticus were determined by a reversed passive latex agglutination assay against tdh. the amount of tdh excreted in growth medium containing either glycocholic acid or taurocholic acid (5 mm/liter) was, on a per-cell basis, 4- to 16-fold greater than that excreted in medium without the bile acids. the amounts of tdh released from lysed cells gro ... | 1996 | 8702295 |
| restriction fragment length polymorphism of the tdh and trh genes in clinical vibrio parahaemolyticus strains. | the restriction fragment length polymorphism of the genes encoding thermostable direct hemolysin (tdh) and thermostable direct hemolysin-related hemolysin (trh) was analyzed for 137 strains of vibrio parahaemolyticus isolated from specimens from diarrheal patients in thailand. the hindiii restriction fragment patterns of tdh and trh were grouped into five and four types, respectively. a strong association between the restriction fragment patterns of tdh and trh was observed with v. parahaemolyti ... | 1996 | 8727923 |
| changes to erythrocyte membrane cation permeability induced by a bacterial toxin. | vibrio parahaemolyticus secretes an exotoxin prepared as kanagawa haemolysin (kh) which causes marked alterations to the function of epithelial, cardiac and other cell types, but whose cellular mode of action is poorly understood. using human red cells as a model system with radiotracer flux techniques, we have shown that kh (1) markedly elevated the basal leak to k+, (2) raised ca2+ influx, and as a consequence of this, (3) stimulated the ca2+-activated k+ channel. these results suggest that an ... | 1996 | 8596714 |
| subspecies typing of vibrio parahaemolyticus by pulsed-field gel electrophoresis. | vibrio parahaemolyticus is one of the most important food-borne pathogens in taiwan, japan, and other costal regions. we report on the development of a pulsed-field gel electrophoresis (pfge) method for the molecular typing of this pathogen. genomic dna was digested with sfii, and the fragments were resolved on 1% agarose with a contour-clamped homogeneous electric field apparatus set at 190 v and a pulse time of 3 to 80 s. a total of 130 selected isolates obtained from outbreaks during 1993 and ... | 1996 | 8735112 |
| the sodium-driven polar flagellar motor of marine vibrio as the mechanosensor that regulates lateral flagellar expression. | certain marine vibrio species swim in sea water, propelled by a polar flagellum, and swarm over surfaces using numerous lateral flagella. the polar and the lateral flagellar motors are powered by sodium- and proton-motive forces, respectively. the lateral flagella are produced in media of high viscosity, and the relevant viscosity sensor is the polar flagellum. the cell might monitor either the rotation rate of the flagellar motor or the mechanical force applied against the flagellum. to test th ... | 1996 | 8793868 |
| purification, characterization, and pathogenicity of urease produced by vibrio parahaemolyticus. | vibrio parahaemolyticus is a halophilic bacterium associated with gastroenteritis and traveller's diarrhea. the urease-positive, kanagawa-negative v. parahaemolyticus had been isolated from patients and the environment in the pacific northwest, first reported by kelly et al. (5). recently, we purified the urease produced by a clinical isolated of v. parahaemolyticus, and its characterization and pathogenicity has been studied. the urease isolation procedure included a water extraction and anion ... | 1996 | 8852357 |
| selective growth of thermostable direct hemolysin-producing vibrio parahaemolyticus in the alimentary tract of a gastropod, clithon retropictus, at a selected estuary in japan. | thermostable direct hemolysin (tdh)-producing vibrio parahaemolyticus increased in cell number in the alimentary tract of a gastropod. clithon retropictus, from an undetectable level in july to 2.0 x 10(3)/g in september 1991 at the sada estuary in the southern part of japan. it was not detected in microalgae, foods of the gastropod, at the estuary or muddy sediments at a neighboring fishing port. the strain was not detected at two estuaries and two fishing ports ecologically separated from the ... | 1996 | 8898295 |
| survival of vibrio parahaemolyticus at low temperatures under starvation conditions and subsequent resuscitation of viable, nonculturable cells. | morphological changes of vibrio parahaemolyticus from rods to spheres took place after a culture was subjected to starvation at a wide range of temperatures. scanning electron micrographs revealed that starved spherical cells gradually developed a rippled cell surface with blebs and an extracellular filamentous substance adhesive to the cell surface. cells starved at a low temperature for certain intervals were counted by various bacterial enumeration methods, including plate count, direct viabl ... | 1996 | 8919790 |
| [an ecological study for prediction of vibrio parahaemolyticus food poisoning in shizuoka prefecture]. | the mean mpn viable cell counts in 15 samples of sea water in which clams were held at the time of the onset of mass outbreaks of food poisoning, the number of food poisoning outbreaks, and prevalence of kanagawa phenomenon-positive strains, and effectiveness of measures to control food poisoning were investigated over 6 years from 1990 to 1995. the results obtained were as follows: 1. of 6 materials, including sea water and shellfish, which were examined to determine the best marker material fo ... | 1996 | 8940834 |
| utilization of iron sources and its possible roles in the pathogenesis of vibrio parahaemolyticus. | vibrio parahaemolyticus is an important enteropathogen in japan, taiwan and other coastal regions. the influence of the regulation of iron on the pathogenesis of this pathogen has not been well characterized. the growth of pathogenic and non-pathogenic strains of v. parahaemolyticus on iron-limited agar plates was stimulated by ferritin, lactoferrin and transferrin at 30 microm, and also by hemin, hemoglobin and ferric ammonium citrate at 100 microm. spontaneous iron-utilizing mutant strains (mu ... | 1996 | 8985934 |
| survey on the distribution of vibrionaceae at the seaport areas in taiwan, 1991-1994. | a monthly survey on the distribution of human-pathogenic vibrionaceae of the seawater from five principal harbors in taiwan was conducted by national quarantine service from july, 1991 to february, 1994. of the total 1,167 vibrionaceae isolates, strains of vibrio alginolyticus (449 strains) were the most frequently isolated, followed by vibrio parahaemolyticus (262) , aeromonas hydrophila (153), vibrio cholerae non-o1 (86), and vibrio vulnificus (67). none of vibrio cholerae o1 was isolated. the ... | 1996 | 10592802 |
| [k-serotype analyses of vibrio parahaemolyticus isolated in northern taiwan, 1983 through 1993]. | from 1983 through 1993, 786 strains of vibrio parahaemolyticus were collected from food-borne disease outbreaks and sporadic cases of diarrheal illness in northern taiwan, involving 42 k-serotypes. five top leading serotypes were k8 (36.8%), k15 (10.8%), k12 (8.7%), k56 (7.9%) and k63 (4.7%). however, a variation of k-serotypes was found during this study period. from 112 food-borne outbreaks associated with this microorganism, only 54 (48.2%) outbreaks were caused by a single serotype, while 58 ... | 1996 | 10592803 |