Publications

glycoprotein enzymes secreted by aspergillus fumigatus: purification and properties of a second beta-glucosidase.	a second extracellular beta-glucosidase (betalarge) of aspergillus fumigatus was purified to homogeneity and shown to be a glycoprotein, as determined by polyacrylamide gel electrophoresis followed by staining for protein and for carbohydrate. its molecular weight was approximately 340,000 by gel filtration, while sodium dodecyl sulfate-polyacrylamide gel electrophoresis gave an apparent molecular weight of 170,000, suggesting that the enzyme has two subunits. the glucosidase contained covalentl ...	1975	51848
molecular restriction of anti-dnp antibodies induced by (dnp)2-gramicidin s.	the molecular properties of anti-dnp antibodies were studied in 80 rabbits hyperimmunized with (dnp)2-gramicidin s. unimmunized animals or those previously skin-painted with dnfb were subjected to biweekly injections of (dnp)2-gramicidin s. isoelectric focusing revealed that the spectrotypes of the anti-dnp antibodies fell into three classifications; monoclonal, 18 to 21%; biclonal, 21 to 30%; and restricted, 14 to 26%. in each immunization protocol 35% of the rabbits did not respond. longer tim ...	1975	51881
enterotoxigenic escherichia coli and idiopathic diarrhoea in bangladesh.	faecal escherichia coli from bangalee patients with idiopathic diarrhoea were tested in the chinese hamster ovary cell (c.h.o.) assay to detect production of thermolabile enterotoxin (l.t.). c.h.o-positive e. coli produce both l.t. and a thermostable toxin (s.t.). c.h.o.-positive strains were found in 19.2% of all cases and in 70% of the most severely ill patients with non-vibrio cholera. e. coli which produce s.t. alone were identified in a third of the c.h.o.-negative cases. enterotoxigenic e. ...	1975	53601
lack of effect of cholera enterotoxin on electrical resistance of lipid bilayer membranes.		1975	54067
antitoxic immunity in experimental cholera: protection, and serum and local antibody responses in rabbits after enteral and parenteral immunization.	the protective effect of enternal and parenteral immunization with cholera toxin antigen against experimental cholera in rabbits was studied by using the small-bowel loop technique. subcutaneous injection of crude toxin as well as purified toxin or toxoids gave rise to significant protection against toxin challenge. the enhanced resistance to toxin was found to correspond to a many-fold higher magnitude of protection against challenge with live vibrios. in the primary response the protection inc ...	1975	54337
[comparative data for the determination of the quality of the nutrient media for the diagnosis of cholera by means of cultures of vibrio cholerae of various ages].		1975	54498
cell-mediated immune response in mice treated with steroidal contraceptives.	the splenomegaly assay (simonsen, 1962) was standardized using different strains of rats and mice. male wistar rat (donor)-female swiss mouse (host) was found to be the suitable combination that could be employed in subsequent experiments to study the potential of contraceptive steroids to alter cmir. the index of splenomegaly appeared to increase in case of mice treated with combination oral contraceptives (ovulen, ovral or enovid). the differences observed, however, neared significance only in ...	1975	55327
vibrio cholerae flagellar antigens: a serodiagnostic test, functional implications of h-reactivity and taxonomic importance of cross-reactions within the vibrio genus.	serodiagnostic tests for all serotypes of vibrio cholerae using h-antisera were investigated. activity motile cell lines of 155 stock and international reference cultures of human, animal, fish, and halophilic vibrios, aeromonas, comomonas, pseudomonas, salmonella, and escherichia were investigated. without exception, all cholera vibrios (including the nag serotypes) reacted with h sera. positive reactions were obtained specifically (a) within 2 hrs at 52 degrees c in the tube test using thick f ...	1975	55952
[detection of specific o-antigen in atypical cultures of vibrio cholerae with the aid of immunochemical methods of study].		1975	57271
[improvement of the method of the h-agglutination reaction with vibrios on glass].		1975	57276
transplantation of tendons sterilized in formalin vapours and preserved in paraffin oil.		1975	58509
dynamic determinants of letf ventricular diastolic pressure-volume relations in man.	left ventricular (lv) diastolic pressure (p), volume (v), and rate of change of volume (dv/dt) were determined at 16.7 msec intervals in 17 patients (simultaneous micromanometer and single plane volume angiography). four patients had mitral stenosis with atrial fibrillation and 13 patients (three normal, two congestive cardiomyophathy, three lv hypertrophy, and five coronary artery disease) were in normal sinus rhythm. maximum early diastolic filling rates (max dv/dt) in the normal and cardiomyo ...	1975	122921
investigations on the biological activity of extracts of tylopilus felleus (bull. ex fr.) p karst. by means of mycological tests.		1975	123071
inhibition of mitogen stimulation of human peripheral blood leukocytes by vibrio cholerae enterotoxin.	it has been found that the enterotoxin (choleragen) of vibrio cholerae, strain 569b, can interfere with phytohemagglutinin-p, pokeweed mitogen and concanavalin a stimulation of human peripheral blood leukocytes. the viability of cultured cells was not affected by the toxin. the toxin does not interfere with stimulation by direct competition for mitogen receptor sites. the ability of choleragen to inhibit stimulation by mitogens declines with time after initiation of stimulation. the biologically ...	1975	123258
ultrastructural studies on the myocardial capillaries of the experimentally lathyritic rat, protective effect of certain flavonoids.	the ultrastructure of rat myocardial capillaries was studied in the course of experimental lathyrism. endothelial cells were hypertrophic, with a sinuous profile of the plasma membrane facing the lumen and with a consistent increase of pinocytotic vesicles; the nuclei were irregular in shape; atpase activity was no more demonstrable. therefore, various and well distinct structural endothelial mechanisms seem to be primarily involved, causing an alteration of the dynamics of transcellular exchang ...	1975	123669
immunogenic properties of membrane-bound atpase from stable proteus p 18 l-forms : a kinetic study of inhibition by specific antibodies.		1975	123742
enzymic synthesis of steroid sulphates. xi. study of the oestrogen binding site of oestrogen sulphotransferase by affinity labelling with 4-mercuri-17beta-oestradiol.	oistrogen sulphotransferase (3"-phosphoadenylylsulphate: oestrone sulphotransferase, ec 2.8.2.4) contains asingle sulphydryl group thought to be at, or near, the oestrogen-binding site. 4-mercuri-17beta-oestradiol, the activity of the enzyme decreased with increasing concentration of the oestrogen derivative. however, some 40% of the activity remained when all the sulphydryl had reacted to form mercaptide. formation of mercaptide was only marginally decreased in the presence of the substrate 17b ...	1975	123774
evidence that the deferential vein acts as a local transport system for androgen in the rat and the dog.	ligation of the deferential vein of the rat has been shown to bring about a reduction in the androgen-dependent activity of the prostatic rna polymerase enzyme. in the dog, the content of androgens in this vein is of the order of that found in the testicular vein and many times higher than that of peripheral plasma. it seems that the cauda epididymidis alone is sufficient to maintain the ipsilateral lobes of the prostate and seminal vesicles providing an intact ductus deferens and contralateral ...	1975	123961
fatal immune complex glomerulonephritis without deposits.	repeated intravenous injections of egg albumin in rabbits produced small antigen-excess complexes and severe glomerulonephritis. immunoglobulins and complement in the glomeruli were not clearly demonstrated by immunofluorescence; deposits were found to be sparse by electron microscopy. this study demonstrates that soluble immune complexes are responsible for the glomerular reaction. the apparent absence of deposits is thus not sufficient to exclude an immune complex pathogenesis.	1975	123971
effects of renal nerves on renal hemodynamics. i. direct stimulation and carotid occlusion.	renal nerves were stimulated either directly (loop electrodes) or indirectly (bilateral carotid occlusion with maintenance of control blood pressure) in anesthetized dogs to determine the effects on renal blood flow (rbf), glomerular filtration rate (gfr), sodium excretion (unav), and intrarenal distribution of blood flow (microsphere method). direct nerve stimulation decreased rbf 20% in 9 of 10 dogs; but gfr, unav, and intrarenal distribution of blood flow did not change significantly. carotid ...	1975	124020
hepatic mitochondrial function in ketogenic states. diabetes, starvation, and after growth hormone administration.	the study was designed to evaluate hepatic mitochondrial function during ketotic states. the ketogenic models studied were streptozotocin-induced diabetic ketoacidosis, 48 h of starvation, and after growth hormone administration. in the last-mentioned model we observed increased free fatty acids but not ketonemia. oxidative phosphorylation was measured using the citric acid cycle substrates pyruvate and succinate, the amino acid glutamate, a ketone body beta-hydroxybutyrate, and a long-chain fat ...	1975	124319
a comparison between the free grafting of sliced and intact muscles in the rat-1.	transverse slicing is a new technique whereby a mammalian muscle can be freely grafted with success. this method eliminates contamination of the early graft by surviving muscle fibres and allows one to measure the development of contractile properties on a uniform population of regenerating muscle fibres.?ioauthor	1975	124664
immunofluorescent localization of glycogenolytic and glycolytic enzyme proteins and of malate dehydrogenase isozymes in cross-striated skeletal muscle and heart of the rabbit.	specific antisera against glycogen phosphorylase, phosphofructokinase, aldolase, glyceraldehyde-phosphate dehydrogenase, enolase, lactate dehydrogenase, cytosolic and mitochondrial malate dehydrogenase from rabbit muscle were obtained from sheep. the gamma-globulins were used for indirect immunofluorescent localization of the respective enzymes in rabbit skeletal muscle and heart. in stretched skeletal muscle a cross-striation like distribution was observed for all enzymes studied. in the case o ...	1975	124716
inhibition not facilitation of sexual behavior by pcpa.	it has been proposed that estrous behavior in the female rat may be under tonic inhibition by a central serotonergi system. studies conbining estrogen priming and the pharmacological depletion of serotonin have provided some support for this hypothesis. some evidence, however, is not consistent with this hypothesis. in the present study estrogen primed ovariectomized-adrenalectomized rats were administered p-chlorophenylalanine and were tested for lordosis behavior 66 and 70 hr later. lordosis w ...	1975	125426
the effect of uncouplers on catecholamine incorporation by vesicles of chromaffin granules.	it is shown that uncouplers inhibit the incorporation of catecholamines by vesicles of chromaffin granules in parallel with their stimulatory effect on the membrane-bound adenosine triphosphatase.	1975	125589
a review of the role of swine skeletal muscle in malignant hyperthermia.		1975	125736
response of frozen and stored canine lymphocytes in culture after thawing.		1975	125944
protection against cholera. a bactericidal mechanism on the mucosal surface of the small intestine of mice.		1975	126054
reversible and irreversible phases of serotonin depletion by 4-chloroamphetamine.	pretreatment of rats with an agent that inhibits uptake into serotoninergic neurons [lilly 110140: 3-(p-trifluoromethylphenoxy)-n-methyl-3-phenylpropylamine hydrochloride] prevented the depletion of brain serotonin by 4-chloroamphetamine, presumably by preventing the entry of 4-chloroamphetamine into the serotonin neuron. when the uptake inhibitor was given after 4-chloroamphetamine, the lowering of both serotonin and tryptophan hydroxylase levels in brain was reversed. serotonin levels returned ...	1975	126166
[clinical conference: hl-a antigens in chronic myeloid leukemia].		1975	126439
[clinical conference: histamine and basophils after cellular separation on albumin gradient].		1975	126441
immunochemical studies with synthetic immunogens chemically related to peptidoglycan.	rabbits were immunized with synthetic immunogens hsa-(gly-l-ala-l-ala-d-ala-d-ala)39 and hsa-(gly-gamma-d-glu-l-ala-d-ala-d-ala)40, respectively. antibodies against hsa-(gly-l-ala-l-ala-d-ala-d-ala)39 showed a strong precipitin reaction with the homologous antigen, with hsa-(gly-gamma-d-glu-l-ala-d-ala-d-ala)40 and with solubilized peptidoglycan containing peptide subunits with c-terminal d-alanyl-d-alanine. the albumin-peptide conjugates also cross-reacted with rabbit antisera to streptococcus ...	1975	126550
cell-mediated immune reactions in vitro to cell walls and peptidoglycan from staphylococcus aureus.	migration inhibition factor (mif) was produced by peritoneal exudate (pe) cells from guinea pigs sensitized with heat-killed cells of staphylococcus aureus cultured in vitro with staphylococcal cell walls or defined subunits of the cell walls. mif activity was assayed by inhibition of migration of alveolar macrophages from lungs of normal guinea pigs. specificity of inhibition was established using pe cells from tuberculin sensitive guinea pigs and tuberculin as appropriate controls. staphylococ ...	1975	126563
inhibition of excision repair without influence upon uv-sensitivity and uv-mutability in escherichia coli b/r hcr+.	pre-irradiation starvation of exponentially growing escherichia coli b/r hcr+ thy-try- strain for thymine and tryptophan causes inhibition of pyrimidine dimer excision from ultraviolet damaged cells dna. this inhibition of excision repair has not resulted in increasing ultraviolet sensitivity nor in increasing frequency of ultraviolet induced tryptophan revertants. the possible mechanisms of the non-excision repair in the prestarved cells, which is at least as accurate and effective as the whole ...	1975	126583
effect of zinc and cadmium on delta-aminolevulinate dehydratase of red blood cells in protecting against enzyme losses during storage.	the effect of zinc and cadmium on delta-aminolevulinate dehydratase of bovine erythrocytes stored at -30 degrees c for different times was determined. the results show a. storage of erythrocytes leads to an enhancement of the enzyme activity, which after six weeks is 165% (500 mum zncl2) respectively 220% (100 mum cdcl2) for red blood cells of calves, and after four weeks in 420% respectively 450% (same concentrations) for red blood cells of adult cattle, b. the older the samples are, the higher ...	1975	126587
neuraminidase treatment enhances the lysolecithin induced intercellular adhesion of amoeba proteus.	egg lysolecithin induces intercellular adhesion of amoeba proteus. pre-treatment of cells with vibrio cholerae neuraminidase (vcn) increases the adhesive property of cells as was evidenced in their formation of larger cell aggregates than controls. a possible role of vcn exposed receptor sites in cellular adhesion is suggested.	1975	126599
[ecology of the cholera vibrions].		1975	126601
biogenesis of mitochondria. the effects of membrane unsaturated fatty acid content on the activity and assembly of the yeast mitochondrial protein-synthesizing system.		1975	126665
[influence of culture on evolution of biological structures in human groups].		1975	126716
[effect of some antifibrillatory agents on the isolated rabbit auricle under hypothermia].		1975	126723
[optical microscopic study of the effect of the hypothalamic hormone t.r.h. on certain cell types of rat anterior hypophysis].	a cytological and optical microscopic study of the anterior lobe of hypohysis in the wistar adult rat 10, 30 or 60 minutes after intravenous injection of 50 mug of synthetic trh discloses modifications of thyrotropic cells and prolactin cells. it's possible to recognize a quick degranulation of the prolactin cells (with a maximum at the 10th minute) and a slower degranulation of the thyrotropic cells (perceptible at the 10th minute and very pronounced at the 60th minute). the somatrotopic cells ...	1975	126765
[effect of a beryllium complex on growth of pseudomonas fluorescens (types r and s). i. influence on the lag phase].	the toxicity of beryllium for pseudomonas fluorescens is studied with the aid of an anionic complex of the element which is stable in peptone medium at ph 6 during the period of investigation. the toxic effect is characterized by an increase in the lag phase which is proportional to the square of the beryllium concentration. further, a process of progressive adaptation is observed when the concentration of the beryllium complex is gradually increased.	1975	126778
letter: safety of retinoic acid in acne therapy.		1975	126796
[developmental aspects of organism immunoreactivity in vaccination against cholera (author's transl)].		1975	126811
effect of methysergide, a blocker of serotonin receptors, on plasma prolactin levels in lactating and ovariectomized rats.	the effect of methysergide (mes, 2.5 mg/100 g body wt), a serotonin antagonist, on prolactin release has been studied in lactating and ovariectomized rats. mes caused significant increases in prolactin release in both animals. studies in ovariectomized, hypophysectomized rats indicate that this effect is not due to a decrease in the peripheral metabolism of prolactin. in vitro incubations of anterior pituitary fragments with mes failed to demonstrate any increase in prolactin release, suggesting ...	1975	126860
tightness and orientation of vesicles from guinea-pig kidney estimated from reactions of adenosine triphosphatase dependent on sodium and potassium ions.	in order to study the "sidedness" of the ligands of the na+, k+-atpase in the phosphorylation from [32p]atp, tight vesicles were prepared from guinea pig kidney and partially purified by a two-stage sucrose and ficoll gradient centrifugation procedure. these vesicles were derived presumably from plasma membrane fragments resealed after the initial disruption of the cells during homogenization. tightness of the vesicles was estimated according to activation by the nonionic detergent, triton x-100 ...	1975	126864
elementary particles in isolated rat liver and spleen nuclear membranes.	elementary spherical particles similar to those described in the mitochondria are found in isolated rat liver and spleen nuclear membranes. the particles are characterized by electron microscopy of sections and preparations negatively stained with phosphotungstic acid or with the atpase histochemical reaction product. the particles exhibit adenosine triphosphatase activity and have a mushroom-like shape with a sphere about 85-90 angstrom in diameter attached to inner face of the inner nuclear me ...	1975	126876
[effect of sensitization on the characteristics of development of experimental atherosclerosis].	tests were set up on 99 rabbits to study the influence of sensitization with the vascular "atherosclerotic" antigen on the features specific for the development of experimental atherosclerosis. the results obtained bear witness to a variable effect of sensitization that depends upon the timing of the latter. sensitization practised concurrently and right from the beginning of feeding the rabbits on cholesterol introduced some elements of prophylaxis of the experimental atherosclerosis. on the ot ...	1975	127064
the significance of coronary arterial stenosis during cardiopulmonary bypass.	myocardial infarction may develop during an uneventful open-heart operation. in order to better understand this complication, we undertook an experimental study. the left circumflex coronary artery of 20 dogs was narrowed to 50 per cent of its area by a metal screw clamp to produce a localized coronary stenosis. regional myocardial perfusion in the distribution of both the stenotic circumflex and normal left anterior descending (lad) coronary arteries was measured by injection of a radioactive-l ...	1975	127092
[certain ways of regulation of the oxidative processes in the heart in myocardial infarct].		1975	127156
antigenic and enzymatic architecture of micrococcus lysodeikticus membranes established by crossed immunoelectrophoresis.	by crossed immunoelectrophoresis with membrane antiserum, 17 antigens have been detected in fractions from plasma membranes of m. lysodeikticus solubilized with triton x-100. absorption tests with protoplasts have demonstrated that eight of the antigens are expressed on the surface. of these antigens the major one has been identified as a succinylated mannan. five of the principal immunoprecipitates unaffected by absorption with protoplasts were shown by zymograms to possess the following enzymi ...	1975	127177
the hurler syndrome: treatment of cultured hurler fibroblasts with normal human serum.	prolonged replacement of fetal calf serum by normal human serum for the enrichment of medium during tissue culture of hurler fibroblasts resulted in increased acid mucopolysaccharides in the cells and in the medium. the predominant intracellular mucopolysaccharide had the characteristics of dermatan sulfate when hurler cells were treated with either serum. normal human serum contains a nonspecific coreective factor capable of augmenting the loss of 35so4-amps from hurler cells, but not from norm ...	1975	127180
[study of anti-t-agglutinins in active and inactivated human sera (author's transl)].	anti-t-agglutinins were found in 252 of 283 human sera investigated applying the two-step neuraminidase test and using an rde-preparation of czechoslovakian origin. storage and heating of the sera was followed by a reduction or loss of t-agglutination dependent on the time of incubation at 4 degrees c and 56 degrees c. this was proved to be caused by the inactivation of the anti-t-agglutinins and not by the loss of complement. t-transformed erythrocytes absorb anti-t-agglutinins completely. howe ...	1975	127453
studies on plasma membranes. xxiii. hormone-like action of concanavalin a on liver plasma membranes: inhibition of (na+-k+)atpase.	concanavalin a inhibits the (na+-k+)-atpase activity of isolated rat-liver plasma membranes, while leaving the mg2+-atpase unaffected. glucagon and cyclic amp act supplementary to the lectin in the inhibition. the lectin effect is counteracted by insulin and l-epinephrine, and is completely abolished by the beta-adrenergic blocking agent propranolol. results are discussed on the basis of the known interactions of concanavalin a with plasma membrane components, including its hormone-like action.	1975	127672
cadmium-induced experimental fanconi syndrome.	we have demonstrated that repetitive injections of cadmium into rats leads to the abrupt development of fanconi syndrome after the renal cortical concentration of cadmium has increased approximately eighty-fold. the syndrome is temporally associated with a fall in both na-k-atpase activity and atp levels, suggesting a possible causal relationship between altered proximal tubular transport and inhibition of the atp-na-k-atpase transport system.	1975	127688
[androgen metabolism and leydig-cell function in acute and chronic liver disease (author's transl)].	diurnal variations of plasma testosterone and urinary excretion rates of testosterone-, androsterone-, aetiocholanolone-, and dha-glucuronide, as well as dha-sulphate were measured before and after selective leydig-cell stimulation (with 3 x 5000 iu human gonadotrophin) in eight patients with alcoholic liver cirrhosis, ten with acute hepatitis and four with haemochromatosis. the circadian variation of plasma testosterone and the maximal testosterone concentration after human gonadotrophin stimul ...	1975	127698
[influence of long-term swimming training on the structure and enzyme activity of myosin in the rat myocardium (author's transl)].	intermittent hemodynamic loading of the rat myocardium due to swimming training for several weeks leads to a significant increase in the specific atpase activity of myosin. this enzymatic alteration of the myosin molecule is accompanied by changes in the stoichiometry of its light chains which are of great significance for the atpase activity. the maximum shortening velocity of the unloaded myocardium (vmax), estimated on the basis of afterloaded contractions, shows a slight increase as a result ...	1975	127740
'immobile' (im), a recessive lethal mutation of xenopus laevis tadpoles.	'immobile' (im) is a recessive lethal mutation discovered in the f3 of a xenopus (xenopus laevis laevis) originating from a mesodermal nucleus of a neurula transplanted into an enucleated egg. the im embryos do not contract after mechanical stimulation nor do they present any spontaneous contraction from the neurula stage onwards. development proceeds normally during the first days after which deformation of the lower jaw and tail are observed. the im tadpoles die when normal controls are at the ...	1975	127824
effect of essential phospholipids on the properties of atpases of isolated rat liver plasma membranes of young and old animals.	"essential" phospholipids (epl), rich in dilinoleyl-phosphatidylcholine were given orally to young and 780-800 day-old rats. the double-labelled (14c/32p) dilinoleyl-phosphatidylcholine was incorporated in plasma membranes and the amounts incorporated into different organs investigated. old animals incorporated higher radioactivity than young. the 14c/32p ratio however decreased in comparison with the substance administered. the amount of (na+-k+)-atpase in rat liver plasma membranes of old anim ...	1975	127894
[histochemical and quantitative studies of rna in the thyroid epithelium under the influence of thyrotropic hormone (tsh)].		1975	127942
a quantitative model of venous stasis thrombosis in rats.	a quantitative model of stasis-type of venous thrombosis in rats is described. the ligated bowel loop was used after provocation by an injection of kaolin. the mesenteric vessels of the loop were cut in a dish filled with distilled water and the extinction of escaped haemoglobin was measured photometrically. heparin was highly effective in this model. vessel wall lesion may be used or inducing thrombosis instead of kaolin.	1975	128018
[vaccination strategy in influenza].	the present paper deals with the conditions that influenza vaccines must comply with, their efficiency in terms of the route of administration, the major antigenic changes of influenza viruses, that has to be known from beforehand, a basic problem for the efficient control of a pandemic. vaccination must have in view reduction of the mortality and morbidity rate, a major criterion in establishing a programme for the prevention of influenza by vaccination.	1975	128101
[supplementary data on the theory concerning the cyclic evolution of influenza viruses].	the distribution per age groups of antibodies to influenza viruses a/hong kong/1/68, a/england/837/69 and a/england/42/72 in the sera collected in 1970 demonstrates that antigenically similar strains also dominated the epidemiologic picture of influenza in the past century. the results suggest that the pandemic of 1893 and infection in persons born before 1885 were caused by a strain similar to a/hong kong/1/68 and in those born in 1895-1897 by the antigenic variant a/england/42/72. the anamnest ...	1975	128105
physiopathological aspect of glynn's type of experimental arthritis.	knee joint arthritis is induced among rabbits previously immunized by intradermal injection with egg albumin (ea) emulsified in adjuvant containing either m. tuberculosis or m. butyricum, then by intra-articular injection with ea. arthritis evolution involves two phases, an early one during the first 2 months and a late one from 3 months to 1 year. during the early phase, arthritis intensities are similar no matter which mycobacterium is used. however, during the late phase, only rabbits immuniz ...	1975	128112
in vitro fertilisation of human oöcytes.	this paper describes attempts to cure infertility due to irremediable fallopian tube lesions by the laparoscopic recovery of pre-ovulatory ovarian oöcytes, their fertilisation in vitro, and their subsequent reimplantation into the uterine cavity.	1975	128140
[some properties of preparations of na+ and k+-atpase treated with deoxycholate].	the treatment of membrane suspension from the guinea pig kidney cortex possessing na+, + k+ -atpase activity with 0.20% sodium deoxycholate (dox : protein equals 12 : 1) leads to lowering of their enzymatic activity. dox-treated preparation can be divided into two fractions by centrigugation (1 hr - 22 000 g). the pellet has 1.5-fold higher enzymatic activity than the initial preparation and cannot be activated by small amounts of histones. the supernatant has 2-fold lower enzymatic activity tha ...	1975	128167
[histological, histoenzymatic and biochemical study of the liver and small intestine of rats during short periods of starvation].	the structure and the activity of acid phosphatase, beta-glucuronidase, alkaline phosphatase and atp-ase in the liver and small intestine of rats receiving for 20 days a one-time, fixed at a certain time (2 o'clock) feeding was studied morphologically in dynamics in 2, 6, 24 and 48 hours after the last feeding. furthermore, parallel with this the activity of acid phosphatase, beta-glucuronidase, alpha-glucosidase and beta-acetylglucosaminidase was determined in homogenates by biochemical methods ...	1975	128202
repair of massive septic abdominal wall defects with marlex mesh.	marlex mesh was used to close the abdominal wall defect in six patients with septic wound dehiscence and intra-abdominal infection. the mesh was implanted under local anesthesia and served as a protective covering for the bowel and allowed early ambulation, including prone positioning of the patient for easier wound care. in four surviving patients, the marlex mesh was covered by full thickness skin flaps after granulation tissue had covered the material. no patients had infected sinus tract for ...	1975	128301
editorial: subunits of myosin. relations to atpase activity and mechanical function of muscle.	under certain conditions the specific atpase activity of myosin of a given muscle can be altered. the cause of this alteration can only lie in the myosin molecule itself. to produce an enzymatic activity of myosin, an interaction between their light and heavy chains is necessary. however, the specific activity appears to be determined mainly by light chains. hence, one ought also to look for a basis of the changed activity in changes of the subunits of myosin. there are strong indications that t ...	1975	128346
ascorbic acid and glycosaminoglycan and lipid metabolism in guinea pigs fed normal and atherogenic diets.	the effect of low and high doses of ascorbic acid on glycosaminoglycan and lipid metabolism was studied in guinea pigs fed both normal and atherogenic diets. the high dose of ascorbic acid (25 mg/100 g body weight/day) decreased the cholesterol level in the liver and aorta but not in the serum in animals fed the normal diet in comparison with those fed the low dose of ascorbic acid (0.1 mg/100 g body weight/day). in animals fed the atherogenic diet, cholesterol decreased in the serum and liver, ...	1975	128367
[organization of the veterinary service of a dairy complex].		1975	129913
cholericidic effect of some intestinal disinfectants.	several compounds have been found which are markedly more effective and more economical as chemoprophylactic agents than the tetracycline compounds traditionally used in cholera-endemic areas. some synergetically acting combinations were shown to exert by several orders of magnitude higher vibriocidal potency than the sum of potency of the compounds when applied singly. in the presence of human intestinal content, however, markedly higher concentrations are needed to exert the proper vibriocidic ...	1975	130761
the acid mucopolysaccharides (glycosaminoglycans) in secondary cultures of embryonic rat fibroblasts.	in secondary cultures of embryonic rat fibroblasts the protein-free acid mucopolysaccharides were separated by cetylpyridinium chloride fractionation and identified afterwards. the following acid mucopolysaccharides were found: hyaluronic acid, chondroitin, 2 heparan sulphate fractions, chondroitin-4-sulphate, chondroitin-6-sulphate, and dermatan sulphate.	1975	134903
[effects of 4-hydroxypentenal on the noncancerous and the cancerous ectocervix uteri hominis (author's transl)].	problem: 4-hydroxy-2,3-transpentenal (hpe) represents the 5-c-homologue of a series of hitherto unknown alpha,beta-unsaturated aldehydes. the marked inhibition of dna-biosynthesis by these chemical compounds, particularly by hpe, suggests blocking of mitosis by hpe due to direct action on the s-phase. it is known that dna-biosynthesis as well as energy metabolism are generally more disturbed by hpe in animal tumor cells (ehrlich ascites and solid tumors, nk/ly sarcoma 37, sarcoma 180, plasmocyto ...	1975	134905
acid mucopolysaccharides in metal-induced tumor and connective tissue capsules surrounding implants in rats.		1975	142824
classification of enterotoxins on the basis of activity in cell culture.	two cell culture systems were used in a study of the biological properties of several bacterial enterotoxins in vitro. by means of one model, in which hela cell monolayers were used, cytotoxic effects, interms of detachment of cells from a glass surface due to cell death, were assayed. by means of the second model, activation of the adenyl cyclase-cyclic adenosine 3', 5'-monophosphate (amp) system, in terms of increased steroidogenesis by y-1 adrenal cells (an effect which we have termed cytoton ...	1975	162926
stimulation of cyclic adenosine 3':5'-monophosphate and corticosterone formation in isolated rat adrenal cells by cholera enterotoxin. comparison with the effects of acth.	1. the production of cyclic adenosine 3':5'-monophosphate (cyclic amp) and corticosterone isolated ratadrenal cells was increased by cholera enterotoxin. both responses were accompanied by a lag period which is characteristic of other known actions of enterotoxin. the duration of the lag period in the production of corticosterone depended on the concentration of enterotoxin; with the maximally stimulating amounts it was 30-45 min. 2. maximum rates of cyclic amp and corticosterone synthesis, afte ...	1975	163102
adenosine 3',5'-cyclic monophosphate in vibrio cholerae.	the extracellular concentration of cyclic adenosine 3',5'-monophosphate (amp) of three different strains of vibrio cholerae growing in syncase medium were measured. cyclic amp secreted by v. cholerae 569b varied widely, with different carbon sources. mutant 13, which produced little or no toxin, released half the amount of cyclic amp as the wild type. the release of less cyclic amp into the medium by mutant 13 may be accounted for by the lower activity of adenylate cyclase observed. high glucose ...	1975	163219
modulation of in vivo antibody responses by cholera toxin.	treatment of mice with an exotoxin (0.01 mug to 1.0 mug) purified from vibrio cholerae culture filtrates markedly influenced the immune response to sheep erythrocytes (srbc) and the escherichia coli lipopolysaccharide (lps). simultaneous administration of the toxin (ct) with antigen resulted in a delayed appearance of antibody plaque-forming cells (pfc) during the first few days after immunization, followed by a marked enhancement of both igm and igg pfc. the secondary immune response to srbc wa ...	1975	163279
selection of a variant lymphoma cell deficient in adenylate cyclase.	isoproterenol, a stimulator of adenylate cyclase, was used to select a stable variant clone of mouse lymphosarcoma cells deficient in the enzyme. the inability of four different stimulators to activate cyclic adenosine monophosphate synthesis in the variant, in contrast to its wild-type parent, implies that in normal cells one type of adenylate cyclase molecular can respond to different activators.	1975	163487
inactivation of viruses and bacteria by ozone, with and without sonication.	selected organisms with public health significance were placed in a reaction chamber for treatment by ozonation, by ozonation and sonication, by sonication, or by sonication during oxygenation. vesicular stomatitis virus, encephalomyocarditis virus, gdvii virus, staphylococcus aureus, pseudomonas fluorescens, salmonella typhimurium, enteropathogenic escherichia coli, vibrio cholerae, and shigella flexneri were inactivated by treatment with ozone. when microorganisms were suspended in phosphate-b ...	1975	163616
structural studies on carcinoembryonic antigen. periodate oxidation.	periodate oxidation has been applied to examine the carbohydrate structure of carcinoembryonic antigen (cea) and the possible role of the carbohydrate residues in its antigenic activity. sialic acid (n-acetylneuraminic acid) and fucose were completely destroyed, and galactose and mannose were partially destroyed by a single periodate treatment. serial periodate treatment (smith degradation) destroyed additional amounts of galactose and mannose as well as significant amounts of n-acetylglucosamin ...	1975	163645
antigenic relationship between medullary thymocytes and a subpopulation of peripheral t cells in the rat: description of a masked antigen.		1975	163704
immunological comparison of various human pregnancy-associated plasma proteins.	direct immunodiffusion comparison with specific antisera demonstrated that all of the four pregnancy-associated plasma proteins (papps) described in our laboratory are distinct from the pregnancy zone protein (von schoultz); alpha2-pregnoglobulin (berne); pregnancy-associated alpha2-glycoprotein (sp3) (bohn); new serum alpha2-macroglobulin (stimson); pag (horne); pregnancy-associated alpha2-globulin (kasukawa); pal (mclaren), and xh protein (dunston). all the latter proved to be immunologically ...	1975	163802
regulation of the in vitro early anamnestic antibody response by exogenous cholera enterotoxin and cyclic amp.	keyhole limpet hemocyanin was injected into the hind foot pads of rabbits. six days later cell suspensions were prepared from the popliteal lymph nodes. various amounts of hemocyanin (1 ng to 100 mug) were added to 1 times 10-7 cells to induce an anamnestic antibody response. various amounts of cholera enterotoxin, which stimulates the enzyme adenylate cyclase, or dibutyryl cyclic adenosine 3'5'-monophosphate (amp), were added to the cultures with or without hemocyanin. de novo synthesis of anti ...	1975	163858
mechanism of action of cholera toxin and the mobile receptor theory of hormone receptor-adenylate cyclase interactions.	rat liver membrane adenylate cyclase (ec 4.6.1.1) that has been stimulated more than 10-fold by cholera toxin (choleragen) has a 3-fold greater sensitivity to stimulation by glucagon. choleragen similarly increases the sensitivity of cyclase to other peptide (acth, vasoactive intestinal polypeptide) and nonpeptide (catecholamines) hormones in this and other tissues. the rate of 125i-labeled glucagon-membrane dissociation is decreased about 2-fold in toxin-treated liver membranes. toxin-activated ...	1975	164020
effects of proteases and neuraminidase on rbc surface charge and agglutination. a kinetic study.	electrophoretic mobility, membrane sialic acid content and agglutinability by "incomplete" antisera against rh-o, hr' and k antigens were determined for red blood cells in the course of treatment with trypsin, ficin and neuraminidase. neuraminidase gradually produces a slight to moderate agglutinability as it reduced surface charge density in proportion to the amount of sialic acid removed. proteases acted in two distinct steps. the first stage is characterized by the cells rapidly becoming high ...	1975	164087
in vitro stimulation of steroidogenesis in rat testis by cholera enterotoxin.		1975	164187
the biogenesis of mitochondrial membranes in the yeast saccharomyces cerevisiae.	membrane lipids of yeast mitochondria have been enriched by growing yeast cells in minimal medium supplemented with specific unsaturated fatty acids as the sole lipid supplement. using the activity of marker enzymes for the outer (kynurenine hydroxylase) and inner (cytochrome c oxidase and oligomycin-sensitive atpase) mitochondrial membranes, arrhenius plots have been constructed using both promitochondria and mitochondria obtained from o2-adapting cells in the presence of a second unsaturated f ...	1975	164216
kinetics of glycerol uptake by the perfused rat liver. membrane transport, phosphorylation and effect on nad redox level.	the kinetics of glycerol uptake by the perfused rat liver were determined according to a model which includes membrane transport, intracellular phosphorylation and competitive inhibition of glycerol phosphorylation by l-glycerol 3-phosphate. the membrane transport obeys first-order kinetics at concentrations below 10 mm in the affluent medium. the k-m of the glycerol phosphorylation was 10 mum and the k-i of the l-glycerol 3-phosphate inhibition was 50 mum. the maximum activity (v) was 3.70 mum ...	1975	164217
cyclic 3',5'-amp phosphodiesterase of rabbit aorta.	cyclic amp and cyclic gmp phosphodiesterase activities (3' : 5'-cyclic amp 5'-nucleotidohydrolase, ec 3.1.4.17) were demonstrated in the isolated intima, media, and adventitia of rabbit aorta. the activity for cyclic amp hydrolysis in the intima was 2.7-fold higher than that for cyclic gmp hydrolysis. the activity for cyclic amp hydrolysis in the media was approximately equal to that for cyclic gmp hydrolysis, but in the adventitia, cyclic gmp hydrolytic activity was 2.1-fold higher than cyclic ...	1975	164219
aspects of hepatic hypoxia: observations on the isolated, perfused pig liver.		1975	164256
lanthanum inhibition of vibrio cholerae and escherichia coli enterotoxin-induced enterosorption and its effects on intestinal mucosa cyclic adenosine 3',5'-monophosphate and cyclic guanosine 3',5'-monophosphate levels.	several trivalent cations, including lanthanum (la3+), inhibited the secretion (enterosorption) induced by the enterotoxins of vibrio cholerae and escherichia coli in the rabbit ileum in vivo. high concentrations (greater than 10 mm) of la3+ were required to inhibit cholera enterotoxin (ce)-induced enterosorption, probably because of the adsorption of the la3+ often potentiated the ce-induced enterosorption. if luminal la3+ exposure followed ce exposure, some recovery of the enterosorptive respo ...	1975	164410
specific susceptibility of sensitized (memory) b cells to suppression and antigenic alteration by murine leukemia virus.	mice infected with murine oncogenic rna viruses, either friend virus (fv) or moloney leukemia virus (mulv-m) reacted well to a primary immunization with antigen(s) of vibrio cholerae; both the kinetics and magnitude of the direct (igm) primary vibriolytic antibody plaque forming cell (pfc) response in the spleen was the same as in non-infected control mice. in contrast, the anamnestic pfc (igm) response of mice preimmunized with cholera and later (2 to 9 months following priming) infected with e ...	1975	164504
lymphocyte depletion induced by cholera toxin; relationship to adrenal cortical function.	intravenous injection of cholera toxin (choleragen) into mice caused a profound lymphocytopenia associated with marked cellular depletion of the lymph nodes, spleen, and thymus. after administration of 1 mu g of choleragen, lymphocytopenia was mot marked at 24 hr; recovery occurred 6 to 10 days later. similarly depletion of lymph nodes and spleen was maximal at 24 hr with recovery by 14 days. choleragen also caused a marked elevation of serum corticosterone and lymphocyte depletion was not obser ...	1975	164505
human very low density lipoproteins: loss of electrophoretic mobility on enzymatic removal of sialic acid residues.	incubation of very low density lipoproteins (vldl) from three hyperlipoproteinemic patients with neuraminidase resulted in a progressive liberation of sialic acid residues. after the original sialic content had been reduced by approx. 75% a lipoprotein fraction having zero electrophoretic mobility on paper appeared. this fraction increased until, after the removal of all the sialic acid residues, the mobility of the entire vldl fraction was reduced to zero.	1975	165020
immune response of cattle to intranasally or parenterally administered parainfluenza type 3 virus vaccines.	the principal significance of pi-3 virus in the bovine respiratory disease complex would seem to be its role in predisposing the respiratory tract to bacterial infection by (5) disrupting the integrity of the superficial mucosa and (3) producing a more favourable milieu for bacterial growth. attention has therefore been given to establishing local immunity to preclude infection of superficial cells of the mucosa. criteria used to evaluate the degree of such immunity are (5) presence of specific ...	1975	165124
local and systemic response after simultaneous intranasal inoculation of temperature-sensitive mutants of parainfluenza 3, ibr and bovine adenovirus 3.	triple seronegative calves were exposed by the nasal route to three (ts) mutants of bovine respiratory viruses (pi3, ibr, adeno3). after a single exposure, they responded with significant levels of serum antibodies to the three viruses. nasal antibodies were demonstrated for pi3 and adenovirus antigens. the failure to demonstrate nasal antibodies to ibr may be due to lack of sensitivity of the procedure used. when reexposed six weeks later, calves had sharp increases in levels of serum antibodie ...	1975	165126
organ cultures of rat and hamster colon.	human fibrin foam as a matrix for three-dimensional organ cultures was successfully employed for the cultivation of newborn rat and hamster colon tissue. colonic tissue was maintained under different oxygen atmospheres and with various synthetic media. explants of rat and hamster colon, maintained in leibovitz's l-15 medium buffered with hepes in the presence of 5% co2 and air, retained their normal columnar epithelial architecture for 24 days in culture. outgrowth of epithelial cells from colon ...	1975	165141
non-producer human cells induced by murine sarcoma virus.	non-produces (np) human cells were isolated from transformed foci induced by the kirsten mouse sarcoma virus. these morphologically altered np cells produced neither infectious virus nor complement-fixing antigens of the murine sarcoma-leukemia virus complex. however, the sarcoma virus genome could be rescued from these nf cells by co-cultivation with cells carrying "helper" kirsten mouse leukemia virus or woolly monkey leukemia virus. the possible usefulness of these cells in efforts designed t ...	1975	165148