Publications

the effects of castration and/or methimazole feeding on the pituitary response to temperature extremes by cockerels.	the pituitary of goitrogen-treated white leghorn cockerels is smaller in size than control birds and the pituitaries of castrated cockerels is nearly twice the size of control birds. the pituitary cells generated by these treatments may not be functional thyrotrophs or gonadotrophs and may not be able to respond to their usual stimuli. low ambient temperature is a well-known stimulus to the thyroid gland acting through pituitary tsh. cyclic-amp-dependent protein kinase activity levels are used h ...	1985	3000864
[results of a trial of a plague monoclonal antibody erythrocyte diagnostic agent].	plague antibody monoclonal erythrocyte diagnosticum was studied in serological tests simultaneously with commercial plague antibody erythrocyte diagnosticum prepared on the basis of hyperimmune horse serum and with commercial plague antigenic erythrocyte diagnosticum. in this investigation the suspensions of numerous strains of yersinia pestis, other closely related and heterologous organisms, experimentally infected wild and laboratory animals, as well as samples of materials obtained from smal ...	1985	2936047
relationship of acth1-39-immunostained fibers and magnocellular neurons in the paraventricular nucleus of rat hypothalamus.	dual antigen immunocytochemical staining procedures were used in the same tissue section to determine the distribution of acth immunostained fibers and varicosities within the magnocellular and parvocellular divisions in the paraventricular nucleus (pvn) of rat hypothalamus and elucidate its anatomical relationship to vasopressin (vp) and oxytocin (oxy)-containing neurons. double immunostained preparations using glucose oxidase-antiglucose oxidase complex combined with pap complex to visualize t ...	1985	3001668
reactivation of latent murine cytomegalovirus from kidney.	cytomegalovirus (cmv) is presumed to cause latent infection, but the sites of infection are incompletely known. we propose that latent murine cytomegalovirus is present in kidney and may be reactivated by explanation. immunosuppressive agents and allogeneic stimuli may enhance this process. balb/c mice were infected 11 to 14 months previously with 10(5) pfu of smith strain murine cytomegalovirus intraperitoneally. kidneys from 15 infected and nine uninfected mice were washed, minced into 1 to 2 ...	1985	3003444
[the afferent pathway of the hippocampus on participation in acupuncture analgesia. a study of retrograde transport method].		1985	3004787
[forestomach carcinoma and hepatoma induced in mice and rats by n-3-methylbutyl-n-1-methylacetonylnitrosamine (mambna)].	mambna is a new n-nitroso compound isolated from fusarium moniliforme-inoculated corn meal. in the present studies the carcinogenicity of mambna is shown by the induction of forestomach carcinomas and liver tumors in mice and rats following the gastric intubation of this compound. among 42 mice of kunming (km) stock treated with mambna (10-20 mg/week), 22 showed epithelial hyperplasia of the esophagus, 29 papillomas and 4 squamous carcinomas in the forestomach, and there were 6 liver adenomas an ...	1985	3004854
influenza viruses as lymphocyte mitogens. i. b cell mitogenesis by influenza a viruses of the h2 and h6 subtypes is controlled by the i-e/c subregion of the major histocompatibility complex.	influenza a viruses of the h2, h3, and h6 subtypes function as t cell-independent b cell mitogens for lymphocytes from balb/c mice. lymphocytes from c57bl/10 mice, however, undergo mitogenesis only in response to h3 viruses. the failure of c57bl/10 lymphocytes to respond to h2 and h6 viruses was shown not to reflect a difference in dose-response profile or kinetics of the response, nor was it due to the activity of suppressor t cells. experiments with congenic and recombinant strains of mice est ...	1985	3155540
non-immune igg f(ab')2 binding to group c and g streptococci is mediated by structures on gamma chains.	the present investigation was designed to determine whether the heavy or the light immunoglobulin chain is involved in the non-immune binding of igg f(ab')2 fragments to specific surface receptors on human group c and g streptococci. purified human polyclonal igg was mildly reduced with dithiothreitol and alkylated with iodoacetamide. light (l) and heavy (h) chains were separated. intact igg and purified l and h chains of polyclonal immunoglobulin g were tested in an inhibition assay for non-imm ...	1985	3156400
[inhibitory effects of bisphosphonates on the resorption of alveolar bone in rats].		1985	3161960
[specific protein in the plasma of rheumatoid arthritis patients].	a unique protein was detected in the plasma of rheumatoid arthritis patients by two-dimensional electrophoresis. we named it rheumatoid arthritis specific protein (rasp). according to the pattern and concentration of its spot, ra patients were classified into three groups as rasp+, rasp +/- and rasp-. rasp+ was detected only in ra and its occurrence was 45/120 (38%). the occurrence of rasp including rasp +/- was 84/120 (70%) in ra, 6/13 (46%) in sle, 2/8 (25%) in pss, 11/51 (22%) in liver diseas ...	1985	3831162
double osteotomy operation in the surgical treatment of coxa plana disease.	during the years 1975 to 1982, what was named the double osteotomy--which is a combination of pelvic osteotomy together with subtrochanteric varus, extension and derotation osteotomy--was applied to 26 cases suffering from coxa plana (perthes-calvé-legg disease). the postoperative follow up averages a period of three years and five months and was undertaken at the department of orthopedics and traumatology of the faculty of medicine, university of ankara, turkey.	1985	3832036
[isolation of yersinia pestis in plague foci in northeast brazil from 1966 to 1982].		1985	3832341
[effect of titanium phosphate, zirconium oxide and a mixture of ammonium phosphotungstenate with zirconium phosphate on the elimination of radioactive cesium from the bodies of rats].		1985	3835674
[effect of polyphenols of coffee pulp on iron absorption].	the effect of the polyphenols of coffee pulp on iron absorption was studied using the method of ligated segments in rats. optimal conditions to measure iron absorption, were determined using as criteria the concentration of fe59 and the time that produced the highest value of blood radioactivity. a concentration of 0.4 uci/dose of fe59 and a 3-hr period were chosen to measure iron absorption. experimental groups were formed assigning six rats randomly to each group. each group was injected with ...	1985	3836605
involvement of iron and copper in biological damage that is induced by oxygen free radicals.		1985	3837800
plasma cell leukemia accompanied by polyclonal gammopathy and congenital tumor in the atrioventricular node.	a 32-year-old man with plasma cell leukemia and congenital tumor of the atrioventricular node is described. although m protein could not be detected by immunoelectrophoresis, the examination of bone marrow atypical plasma cells with immunofluorescent and immunoperoxidase showed that most of these plasma cells produced igg or iga. also some plasma cells possessed concomitantly both igg and iga. an electrocardiogram revealed complete atrioventricular block. the patient died of aspergillosis and bl ...	1985	3837948
[heterogeneity of monoclonal antibody affinity].	in experiments with pasteurella pestis monovalent capsular antigen and hybridoma monoclonal antibodies obtained after cloning and recloning the heterogeneity of the active centers of antibodies with respect to their affinity was revealed. in ascitic fluids obtained from the animals inoculated with different hybridoma clones 5 groups of antibodies, differing in their affinity, were determined in each fluid sample.	1985	3838847
heterogeneity of membrane phospholipid mobility in endothelial cells depends on cell substrate.	cellular growth control and differentiation have been shown to be dependent on both cell-cell and cell-substrate contacts. interactions of cells with extracellular material are critical events during embryonic development and maintenance of tissue function. plasma membrane receptors have been described for components of the extracellular matrix such as fibronectin, laminin and various collagen types. transmembrane signalling has been shown to be influenced by the lateral mobilities of the plasma ...	1985	3839906
the action of the yersinia pestis phage on the plague envelope antigen and the human erythrocytes.	the activity of the yersinia pestis phage on the plague envelope antigen has been described. the purified envelope antigen was shown to possess the receptor properties. the phage caused changes of viscosity and hemagglutination properties of the antigen. the phage-treated antigen showed the additional precipitation arc in the immunoelectrophoretic investigations. the adsorption of the yersinia pestis phage on the sheep and human erythrocytes was examined. the human red cells of group b exhibited ...	1985	3841818
the effect of toosendanin on monkey botulism.		1985	3849628
theoretical mechanisms for synthesis of carcinogen-induced embryonic proteins: xiv. mutational and non-mutational mechanisms as subsets of a more general mechanism. part c. a defined cancer mutation.	the results of applying a mechanism of ethionine-induced embryonic gene derepressions to explain similar features found in hereditary tyrosinemia have been extended to a well defined cancer mutation. in all three cases, the described mechanism is compatible with the explanation for the etiology of embryonic like phenotypic expressions in cells and potentially for the carcinogenic process. the essence of the formulated process for a human bladder carcinoma mutation in the ras gene for a protein p ...	1985	3853079
[the plague].		1985	3854706
quantitative assessment of the role of o6-methylguanine in the initiation of carcinogenesis by methylating agents.	induction of transformation, cell lethality, and dna lesions were quantitatively compared in syrian hamster embryo cells (hec) treated with three different methylating agents: n-methyl-n'-nitro-n-nitrosoguanidine (mnng), n-methyl-n-nitrosourea (mnu), or methyl methanesulfonate (mms). each induced transformation in a dose-dependent manner. on a molar basis, mnng was approximately equal to 100- and 500-fold more effective than mnu and mms, respectively. for each carcinogen the induction and repair ...	1985	3855560
recognition of a leukemia-related antigen by an antiidiotypic antiserum to an anti-gp70 monoclonal antibody.	the possibility that receptors for retroviral gp70 share structural elements with the antigen-binding sites of anti-retroviral gp70 antibodies was investigated. a monoclonal antibody (1416) was produced that reacted with the gp70 of a cloned recombinant leukemogenic retrovirus, termed p1. an antiidiotypic antiserum raised to 1416 was tested for its ability to bind to the thymic leukemia induced by p1 (p1 thy). a membrane structure was identified on the surface of p1 thy that reacted with the ant ...	1985	3856626
human monocyte-endothelial cell interaction in vitro.	we have examined the interaction of freshly isolated human blood monocytes with cultured human umbilical vein endothelial cells in vitro. purified monocytes incubated with confluent primary or passaged endothelial cells (ec) for 90 min at 37 degrees c bound at maximal densities of 6.5-7.0 x 10(3)/mm2 (8 or 9 per ec) without causing disruption of the monolayer. monocyte-ec binding proceeded in the presence of plasma proteins or optimal phagocytic doses of opsonized zymosan particles. the avidity ...	1985	3865221
[community-acquired pneumonias: importance of pneumococcal pathology].	the frequency and the various etiologies of community acquired pneumonia are not well established. in adults, it is believed that half of the cases are caused by viruses and mycoplasma pneumoniae. the pneumonia in these patients is limited and treated on an ambulatory basis. in patients who need to be hospitalized, the main etiology of pneumonia is s. pneumoniae. despite effective antibiotic treatment, pneumococcal pneumonia still remains a life-threatening infection, especially in bacteremic pa ...	1985	3871965
reactivities of neoplastic t cells to human autoantibody and monoclonal antibodies.		1985	3873778
release of interleukin i and low-molecular-weight lymphocyte-activating factors by rat peritoneal macrophages and its enhancement by acute non-specific inflammatory processes.	peritoneal macrophages harvested from rats undergoing an acute non-specific inflammatory reaction induced by an injection of calcium pyrophosphate (capp) into the pleural cavity released increased amounts of interleukin i (il-i)-like material. lymphocyte-activating factors were also found in ultrafiltrates of the macrophage supernatants below 10 kd and 5 kd. a similar pattern of activity was observed when lysates of the macrophages were tested. in addition pre-exposure of normal peritoneal macro ...	1985	3873954
aids: discrimination issues.		1985	3880472
aids: discrimination issues.		1985	3880472
integration of the vwa plasmid into the chromosome of yersinia pestis strains harboring f' plasmids of escherichia coli.	conditional virulent strains of yersinia pestis were used as recipients of an f-lac plasmid from either escherichia coli 23.10s or e. coli csh23. the transconjugants of y. pestis were calcium independent; however, calcium dependence was restored after the loss of the e. coli plasmid. the plasmid contents of several y. pestis f-lac clones were compared with those of the parent strains and several lac segregants. although the 6- and 61-megadalton plasmids were detected in all strains tested, the v ...	1985	3882563
transfer of the virulence plasmid of yersinia pestis to yersinia pseudotuberculosis.	transposon tn5 insertion derivatives of the virulence plasmid pyv019 of yersinia pestis were transferred by p1 transduction into a plasmid-free strain of y. pseudotuberculosis. one of these plasmid derivatives conferred virulence upon the y. pseudotuberculosis strain. this strain had the ability to express temperature-inducible plasmid-coded outer membrane proteins and was also found to be ca2+ dependent.	1985	3884511
cigarette smoking--induced enhancement of platelet function: lack of prevention by aspirin in men with coronary artery disease.	increased cardiovascular morbidity and mortality among cigarette smokers may be mediated in part by enhanced platelet function. previous data showed that cigarette smoking--induced lowering of the platelet aggregate ratio of normal individuals was prevented by taking aspirin before smoking. our study was undertaken to determine whether similar results would occur in men with coronary artery disease and whether platelet factor 4 would be released. a random-order, double-blind crossover study comp ...	1985	3884719
synthesis by mouse peritoneal cells of g-csf, the differentiation inducer for myeloid leukemia cells: stimulation by endotoxin, m-csf and multi-csf.	normal c57bl mouse peritoneal cells were able to synthesize material with the ability to induce differentiation in colonies of the mouse myelomonocytic leukemia cell line, wehi-3b. the active factor was provisionally identified biochemically as the normal regulator, granulocyte colony-stimulating factor, g-csf. thioglycollate-induced peritoneal exudate cells had little or no capacity to synthesize such material. production of active material was elevated 10-100-fold by exposure of peritoneal cel ...	1985	3887043
[fleas immune to plague studied by a serological method].		1985	3887115
[effectiveness of various methods of exterminating fleas in natural foci of plague (review of the literature)].		1985	3887116
[experimental studies on the behavior of intracranial pressure under prostacyclin application].	in recent experimental studies the beneficial therapeutic effect of the arachidonic acid metabolite prostacyclin (pg 12) has been demonstrated for the treatment of different states of impaired brain perfusion following subarachnoid hemorrhage or focal cerebral ischemia. prostacyclin has been recommended especially for the possible prevention and treatment of cerebral vasospasm after aneurysm rupture. because prostacyclin is a potent vasodilator of the cerebral circulation it is an essential prer ...	1985	3887195
[experimental studies on the behavior of intracranial pressure under prostacyclin application].	in recent experimental studies the beneficial therapeutic effect of the arachidonic acid metabolite prostacyclin (pg 12) has been demonstrated for the treatment of different states of impaired brain perfusion following subarachnoid hemorrhage or focal cerebral ischemia. prostacyclin has been recommended especially for the possible prevention and treatment of cerebral vasospasm after aneurysm rupture. because prostacyclin is a potent vasodilator of the cerebral circulation it is an essential prer ...	1985	3887195
ethanol-induced genotoxicity.	unrelated, nondenatured, ethanol preparations, derived synthetically or by fermentation, were found to induce qualitatively similar concentration-dependent toxic and genotoxic effects as measured by rk mutatest. in this system ethanol was found genotoxic above a threshold concentration of 18-19% (v/v) when rk+ selector cells were transiently exposed for 10 min before selection for rk- survivors at 42 degrees c.	1985	3889601
repair of dna single-strand breaks in x-irradiated yeast. ii. kinetics of repair as measured by the dna-unwinding method.	the kinetics of disappearance of single-strand breaks (ssb) from the dna of x-irradiated stationary yeast cells under liquid-holding conditions was found to proceed in a dose-independent manner up to a dose of at least 2400 gy, and was found to be complete after incubation of cells for 1 h. this was deduced from data for a yeast wild-type (wt) haploid and diploid strain as well as for rad52 haploid cells defective in dna double-strand break (dsb) repair. in all cases an initial fast repair compo ...	1985	3889609
tiapamil--a new calcium antagonist.	calcium antagonists are an important new modality in cardiovascular therapy. tiapamil, a congener of verapamil, is undergoing clinical and laboratory evaluation. we have undertaken experimental studies on the anti-arrhythmic properties of tiapamil, and on the intravenous use of this agent in patients with acute myocardial infarction. reasons are given for suggesting that tiapamil warrants further clinical evaluation, after which it may join the more established calcium antagonists as a valuable ...	1985	3890216
a new serine protease which preferentially recognizes p-guanidino-l-phenylalanyl residue in ascitic plasma from ehrlich ascites tumor-bearing mice.	a new enzyme which hydrolyzes anilide substrates of p-guanidino-l-phenylalanine in preference to those of arginine was found in the ascitic plasma from ehrlich ascites tumor-bearing mice. the activity of this enzyme on n alpha-benzyloxycarbonyl-p-guanidino-l-phenylalanine p-nitroanilide was strongly inhibited by diisopropyl fluorophosphate and phenylmethanesulfonyl fluoride but not by sulfhydryl-reactive reagents and metal chelating agents. peptide substrates containing p-guanidino-l-phenylalani ...	1985	3890849
a new serine protease which preferentially recognizes p-guanidino-l-phenylalanyl residue in ascitic plasma from ehrlich ascites tumor-bearing mice.	a new enzyme which hydrolyzes anilide substrates of p-guanidino-l-phenylalanine in preference to those of arginine was found in the ascitic plasma from ehrlich ascites tumor-bearing mice. the activity of this enzyme on n alpha-benzyloxycarbonyl-p-guanidino-l-phenylalanine p-nitroanilide was strongly inhibited by diisopropyl fluorophosphate and phenylmethanesulfonyl fluoride but not by sulfhydryl-reactive reagents and metal chelating agents. peptide substrates containing p-guanidino-l-phenylalani ...	1985	3890849
a possible role for natural killer cells in providing protection against plasmodium berghei in early stages of infection.	beige mutant mice, which are deficient in natural killer (nk) cells, exhibited a significantly higher parasitaemia than the parental c57bl/6 strain between days 4 and 10 after infection with plasmodium berghei. within 8-12 days after infection 70% of beige mice were dead but no deaths occurred in the parental strain until day 16. the median survival time of the beige mice (10 days) was significantly lower than that of the parental strain (22 days). it appears that nk cells may be protective in t ...	1985	3891603
modulation of polymorphonucleate-mediated cytotoxicity against candida albicans by thymosin alpha 1.	in the present work we analyze the effects of thymosin alpha 1 treatment on the number and the candidacidal activity of murine polymorphonuclear leukocytes. the data we obtained showed that the treatment with thymosin alpha 1 (100 micrograms/kg s.c.) 10, 8, 6, 4 and 2 days before the assay may result in a significant numerical augmentation of circulating polymorphonucleates in the peripheral blood, as well as of their candidacidal activity when measured in vitro in both a 4-h cytotoxicity assay ...	1985	3892779
an improved indirect enzyme-linked immunosorbent assay (elisa) for the detection of antisperm antibodies.	an indirect enzyme-linked immunosorbent assay (elisa) was used for the detection of antisperm antibodies which provides a sensitivity and ease of quantitation that is not available with conventional bioassay systems. a standardized protocol was developed in which washed whole sperm were coated onto polystyrene microelisa plates at a density of 1 x 10(5) sperm per well using a commercially available spray fixative. urease was employed in the enzyme-antiimmunoglobulin conjugate to minimize nonspec ...	1985	3893172
protection against yersinia infection induced by non-virulence-plasmid-encoded antigens.	specific immunity against yersinia was induced by plasmid-encoded antigens not associated with virulence. mice were immunised with viable bacteria from a virulence-plasmid-cured strain of y. pseudotuberculosis. this antigenic stimulation generated specific protection against virulence-plasmid-harbouring strains of y. pseudotuberculosis and y. pestis, demonstrating that protection can be generated by organisms lacking plasmid-encoded virulence antigens.	1985	3900406
role of a plasmid in the pathogenicity of yersinia species.		1985	3902382
properties of an escherichia coli mutant deficient in phosphoenolpyruvate carboxylase catalytic activity.	a mutant escherichia coli (ppcc-) which was unable to grow on glucose as a sole carbon source was isolated. this mutant had very low levels of phosphoenolpyruvate carboxylase activity (approximately 5% of the wild type). goat immunoglobulin g prepared against wild-type phosphoenolypyruvate carboxylase cross-reacted with the ppcc- enzyme. the amount of enzyme protein in the mutant cells was similar to that found in wild-type cells, but it had greatly diminished specific activity. the catalyticall ...	1985	3902793
splenectomy in experimental pancreas transplantation.		1985	3904093
[taxonomy of the causative agents of plague and pseudotuberculosis].		1985	3904284
[use of monoclonal antibodies for the production of a plague antibody erythrocyte diagnostic agent].	monoclonal antibodies to yersinia pestis capsular antigen were fixed onto the surface of formulated sheep red blood cells. the preparation thus obtained was compared with commercial antibody erythrocyte diagnosticum in the passive hemagglutination test aimed at the search for the capsular antigen in the suspensions of yersinia pestis museum cultures and in the antigen neutralization test aimed at the search for antibodies in the sera of wild and laboratory animals having had plague. monoclonal e ...	1985	3904286
[use of beta-lactamase from bacillus licheniformis 749/c for solid-phase immunoenzyme analysis].	beta-lactamase from bacillus licheniformis 749/c was used as a marker for elisa. conjugates of the beta-lactamase with the capsule antigen of the plague causative agent and with the monoclonal antibodies to it were prepared by glutaric aldehyde "linking". the conjugates preserved high immunospecific and beta-lactamase activity. high sensitivity of the modification of elisa allowed detecting up to 8 ng/ml of the capsule antigen of the plague causative agent. the enzymatic activity of the beta-lac ...	1985	3904622
morphological and functional changes in several endocrine glands induced by hypothyroidism in the rat.	the effect of hypothyroidism upon the morphology and the function of several endocrine glands was studied in radiothyroidectomized male rats. it was found that t3, t4, insulin, prolactin and corticosterone levels were significantly lower in hypothyroid rats. tsh levels were significantly higher in these animals while no changes were depicted in testosterone levels. the administration of t4 drew back to normal range the above-mentioned altered serum hormone levels. the studies performed with ligh ...	1985	3907250
establishment of pregnancy and its immunological implications in the pig.	the consideration of the fetus as a semi-allograft by virtue of its paternally inherited histocompatibility antigens is generally accepted. many hypotheses have been put forward to explain the immunological acceptance of the conceptus by the maternal immune system, but no single theory has proved to be adequate. most studies so far have been carried out with laboratory rodents and men but corresponding investigations of farm animals and especially the pig are limited. because striking difference ...	1985	3910828
calcitonin cell population and distribution in the thyroid gland of the rat.	calcitonin-containing cells (c cells) were identified in male wistar white rats using an immunoperoxidase technique. they occupied a central position within the thyroid; very few were found peripherally, inferiorly, and superiorly; and none were present in the isthmus. the number of calcitonin-containing cells present per gram of body weight increased with age up to 70 days and had declined by 100 days. determining the true total c-cell count through the entire thyroid is a very laborious proced ...	1985	3910845
relation between antibiotic resistance and number of plasmids in enterotoxigenic and non-enterotoxigenic escherichia coli strains.	we have investigated the relation between antibiotic resistance and the number of plasmids contained in 44 enterotoxigenic (etec) and 69 non-enterotoxigenic (non-etec) escherichia coli strains. both etec and non-etec strains were resistant to one or more antibiotics at a similar rate, but multiple resistance in resistant non-etec strains was higher than in resistant etec strains, showing a significant difference (p less than 0.001) when subjected to student's t test. among the etec strains, thos ...	1985	3911036
[evaluation of the sensitivity of haemophilus to antibiotics by the modified api-atb system].	a simple micromethod in a liquid medium using the api-atb system was developed for testing the susceptibility of haemophilus to antibiotics. to evaluate this method, 50 strains, including 12 beta-lactamase producers, were studied. results were compared to those obtained using mic determination in a liquid medium (reference) and an agar diffusion method (routine). for all three techniques, a mueller-hinton medium enriched in hemoglobin and nad was used, and cultures were incubated at 37 degrees c ...	1985	3911154
[comparative study of lipoteichoic acid from streptococcus pyogenes type 29 isolated by different methods].	a comparative study of lipoteichoic acid preparations extracted from streptococcus pyogenes with cold and hot phenol and trichloracetic acid was made. the most delicate way for isolation of lipoteichoic acid from the given microorganisms is cold phenol extraction.	1985	3911202
photochemical modifications of lac repressor: effect of effectors binding on tryptophan photooxidation.		1985	3911222
[changes in the oxygen-dependent metabolic activity of polymorphonuclear leukocytes from the peripheral blood and macrophages from the peritoneal exudate in the experimental infection of mice with the causative agent of plague].	the subcutaneous infection of c57bl/6j mice and noninbred white mice with 40 ld100 of y. pestis virulent strain has been found to produce synchronous changes in the oxygen-dependent metabolism (odm) of peripheral blood neutrophils in the spontaneous or zymosan-, e. coli- and y. pestis-stimulated variants of the nbt test. these changes can be divided into three phases: (i) the phase of a sharp drop in odm activity; (ii) the phase of the increase of this activity, occurring simultaneously with the ...	1985	3911675
[preparation and use of magnetic sorbents for studying microorganism antigens].	in this work simple techniques for obtaining polyacrylamide sorbents with magnetic properties are described. these techniques have permitted obtaining block and microgranulated sorbents with the immobilization of antibodies from plague antiserum in the cellular gel structure for the specific sorption of killed and live yersinia pestis cells and their first fraction; pig brain gangliosides have also been incorporated into the gel structure with a view to the sorption of cholera toxin from the fil ...	1985	3911684
[serine trypsin-like proteinase from the cells of the causative agent of plague yersinia pestis].		1985	3912146
[purification of the dna-dependent rna-polymerase of yersinia pestis and its characteristic properties].		1985	3916962
biosynthesis of cholestanol from bile acid intermediates in the rabbit and the rat.	biliary 7 alpha-hydroxy-4-cholesten-3-one (an intermediate in bile acid biosynthesis) may be 7 alpha-dehydroxylated in the gut and further metabolized to cholestanol (skrede, s., and björkhem, i. (1982) j. biol. chem. 257, 8363-8367). we have now evaluated the quantitative importance of pathway(s) to cholestanol with 7 alpha-hydroxylated c27 steroids as intermediates. after feeding conventionally fed rabbits or rats or germ-free rats with [7 alpha-3h]cholesterol and [4-14c]cholesterol, tissue ch ...	1985	3917436
modification of bronchial hyperreactivity after treatment with sodium cromoglycate during pollen season.	repeated bronchial histamine challenges before, during, and after the birch pollen season were performed in 22 allergic patients with bronchial hyperreactivity (bhr) treated for 6 wk with sodium cromoglycate (scg), 20 mg, four times a day, or placebo in a double-blind, randomized group comparison. clinical assessments of the asthmatic symptom score and peak expiratory flow revealed less symptoms and less use of bronchodilators in the scg group. responsiveness to histamine was significantly incre ...	1985	3920301
intracellular gas supersaturation tolerances of erythrocytes and resealed ghosts.	intact mammalian, avian, and amphibian erythrocytes were saturated with up to 300 atm nitrogen or argon gas and rapidly decompressed. despite the profuse nucleation of gas bubbles in the suspending fluid, no evidence of intracellular gas bubble nucleation was found; all or most of the cells remained intact and little or no hemoglobin escaped. internal bubbles were similarly absent from resealed ghosts of human erythrocytes as shown by lack of disintegration and by retention of an entrapped fluor ...	1985	3921076
regulation of cardiac vulnerability by the cerebral defense system.	psychosocial stressors are risk factors for sudden cardiac death. a theoretical model of the brain mechanism that links defined environmental events (stressors) to cardiac vulnerability (initiation of ventricular fibrillation) has been developed. in the model, a stressor event evokes a set of electrochemical responses in the frontal cortex. depending on the state of acquisition of these electrochemical responses to the stressor, activity will or will not be initiated in the frontocortical-brains ...	1985	3923079
effect of mannitol on cerebral blood flow and cerebral perfusion pressure in human head injury.	patients with severe head injury frequently have evidence of elevated intracranial pressure (icp) and ischemic neuronal damage at autopsy. mannitol has been used clinically to reduce icp with varying success, and it is possible that it is more effective in some types of head injury than in others. the aim of the present study was to determine the effect of mannitol on icp, cerebral perfusion pressure (cpp), and cerebral blood flow (cbf) in patients with severe head injury, and to discover if the ...	1985	3925092
[characteristics of the capsular antigen preparations isolated from a broth culture of yersinia pestis strain ev].	the physico-chemical and immunobiological study of the preparation of capsular antigen, isolated by a high-yield method from the culture fluid of y. pestis grown in nutrient broth at 37 degrees c and practically free from the admixture of polysaccharide and nucleic acids, has been made. the study has revealed that this capsular antigen is protein with a molecular weight of 1.65-1.69 x 10(6) d and sedimentation constant equal to 13.4s; its viscosity is 220 ml/g and its isoelectric point is at ph ...	1985	3925663
pressure- and temperature-dependent adhesion of pseudomonas aeruginosa to hep-2 cells.	pseudomonas aeruginosa bacteria adhere to human epitheloid (hep-2) cells in culture. under normal atmospheric conditions (1 ata), this adhesion increased significantly when the temperature rose from 22 to 37 degrees c. under hyperbaric atmosphere (= air, 7 ata) conditions, a similar, significant enhancement of bacterial adhesion to the cells was noted when the temperature rose. if the temperature was kept stable at 22 or 37 degrees c and the pressure was increased from 1 to 7 ata, a pressure-ind ...	1985	3925713
[pharmacological study of benzylpenicillin-benzamido-methyl ester. i. in vivo chemotherapeutic properties].		1985	3925715
the effects of aerosols of carbamylcholine, serotonin and propranolol on the ventilatory response to co2 in guinea pigs and comparison with the effects of histamine and sulfuric acid.	a new method is introduced to recognize the acute pulmonary effect of airborne contaminants in guinea pigs. the method avoids anaesthesia and invasive techniques and requires minimal restraint of the animal. to recognize the acute pulmonary effect, the inspiratory volume of the animal was increased by continuous inhalation of a mixture containing 10% co2 in 18% o2 and 72% n2. once a plateau increase in inspiratory volume was reached, the aerosol to be studied was added to this mixture and its ef ...	1985	3925716
limitations, risks and complications of laser recanalization: a cautious approach warranted.		1985	3925749
effect of naloxone on luteinizing hormone, follicle-stimulating hormone, and prolactin secretion in the different phases of the estrous cycle.	it is becoming increasingly clear that the effects exerted by opioid agonists and antagonists on the release of gonadotropins and of prl may vary according to the endocrine milieu. to investigate this issue further, female rats with a regular 4-day estrous cycle have been injected sc with the opioid antagonist naloxone at different hours of the day, during each of the various days of the estrous cycle. the animals were killed 20 min after the sc administration of naloxone (2.5 mg/kg dissolved in ...	1985	3926461
experimental intestinal obstruction in cattle. part i: changes in the clinical picture.		1985	3927617
spectroscopic studies of pyruvate oxidase flavoprotein from escherichia coli trapped in the lipid-activated form by cross-linking.	pyruvate oxidase is a flavoprotein dehydrogenase isolated from escherichia coli which catalyzes the oxidative decarboxylation of pyruvate to acetate plus co2. the maximal turnover of the enzyme, measured using a ferricyanide reductase assay, is increased 20-to 30-fold by either of two methods. proteolysis in the presence of the substrate (pyruvate) and cofactor (mg2+-thiamin pyrophosphate) results in cleavage at a single locus near the carboxyl terminus and concomitant activation. phospholipids ...	1985	3928620
identification of proteolytic pseudomonads isolated from raw milk.	proteolytic pseudomonads isolated from raw milk were classified by numerical taxonomy. unweighted pair-group average-linkage cluster analysis was used to cluster 49 bacterial strains, of which 26 were pseudomonas species, as described in the bergey's manual of determinative bacteriology, based on 52 characters. the milk isolates resided in two clusters; one containing pseudomonas fluorescens and the other pseudomonas fragi. the isolates identified with pseudomonas fluorescens hydrolyzed milk pro ...	1985	3930583
synthesis of dihomoprostaglandins from adrenic acid (7,10,13,16-docosatetraenoic acid) by human endothelial cells.	human umbilical vein endothelial cells were found to contain adrenic acid (22:4) in their cellular lipids. since this fatty acid may be metabolized by cyclooxygenase in the kidney, the metabolism of adrenic acid was studied in endothelial cell cultures. [14c]adrenic acid was metabolized to several more polar metabolites. two of these metabolites co-migrated on hplc with 1 alpha,1 beta-dihomo-8-ketoprostaglandin f1 alpha (the metabolite of 1 alpha, 1 beta-dihomoprostaglandin i2) and 1 alpha,1 bet ...	1985	3931686
penetration of aflatoxins through isolated human epidermis.	the purpose of this study was to determine if aflatoxin b1 (afb1) could penetrate through isolated human epidermis (stratum corneum plus viable epidermis). [14c]afb1 (7.5-9.3 micrograms) was applied to the stratum corneum of epidermal disks mounted in teflon diffusion cells. [14c]afb1 penetrated chemically unaltered through the isolated epidermis. chloroform-extractable radioactivity accounted for 82.5 +/- 3.7% of the total penetrating radioactivity in the receptor fluid of the diffusion cells. ...	1985	3932668
goat's milk: legal, disease risk and microbiological considerations.		1985	3934373
goat's milk: legal, disease risk and microbiological considerations.		1985	3934373
plague in the united states, 1984.		1985	3934524
follicle regulatory protein noncompetitively inhibits microsomal 3 beta-hydroxysteroid dehydrogenase activity.	a heat- and trypsin-labile follicular fluid protein (frp) extracted from both human and porcine follicular fluid has been shown to modulate ovarian steroidogenesis. to further investigate the effects of frp, its effect on the kinetics of 3 beta-hydroxysteroid dehydrogenase activity (3 beta-hsd) was evaluated in cell-free microsomal preparations from human placenta. test fractions of follicular fluid protein were preincubated with placental microsomes followed by the addition of various substrate ...	1985	3935862
inhibition of the differentiation of candida albicans by the chelator 1,10-phenanthroline.	several chelators were examined for their ability to prevent the synchronous release of 24- to 48-hour stationary phase singlet cells of the dimorphic yeast candida albicans into either the mycelial or the budding phenotypes (in a defined liquid medium at 37 degrees c; at ph 6.5 or ph 4.5, respectively). the only chelator that was found to inhibit mycelium formation completely and to restrict bud formation to about 10% was 1,10-phenanthroline at minimal concentrations of 50 microm and 230 microm ...	1985	3937057
comparative efficiency of primary light conversion in photosynthetic bacteria rhodospirillum rubrum and rhodopseudomonas viridis.		1985	3939722
expression of antigens encoded by the virulence plasmid of yersinia enterocolitica under different growth conditions.	the expression of polypeptides of the virulence plasmid of yersinia enterocolitica serotype o:3 was studied with the immunoblotting technique and specifically absorbed antisera to y. enterocolitica o:3. at least 16 polypeptides were apparently specified by the virulence plasmid when plasmid-bearing bacterial were grown at 37 degrees c or intraperitoneally in semipermeable capsules. the different growth media used (also with added ca2+) had quantitatively or qualitatively only a little influence ...	1985	3965395
survival and growth of yersinia pestis within macrophages and an effect of the loss of the 47-megadalton plasmid on growth in macrophages.	the survival and growth of yersinia pestis cells within mouse peritoneal cavities and within mouse peritoneal macrophages maintained in vitro was examined. two strains were used which differed only in that one (kim) contained the 47-megadalton plasmid associated with virulence and the second (kim1) lacked this plasmid. the kim cells, but not the kim1 cells, acquired some resistance to phagocytosis during growth at 37 degrees c which was not evident when cells were grown at 26 degrees c. whether ...	1985	3965398
the effect of heat shock on the cell cycle regulation of tubulin expression in physarum polycephalum.	in the myxomycete physarum polycephalum, tubulin synthesis is subject to mitotic cycle control. virtually all tubulin synthesis is limited to a 2-h period immediately preceding mitosis, and the peak of tubulin protein synthesis is accompanied by a parallel increase in the level of tubulin mrna. the mechanism by which the accumulation of tubulin mrna is turned on and off is not clear. to probe the relationship between tubulin regulation and cell cycle controls, we have used heat shocks to delay m ...	1985	3968184
reversible and irreversible interactions between elastin and plasma lipoproteins.	the interactions between radiolabeled, human plasma lipoproteins and elastin derived from bovine ligamentum nuchae were investigated using a washout technique. the interaction was characterised by ki, a coefficient of irreversible binding, and kr, the reversible partition coefficient. for both low-density lipoproteins (ldl) and high-density lipoproteins (hdl) the ki values decreased as total lipoprotein concentration increased, suggesting that the binding is saturable, and were similar in magnit ...	1985	3970975
lesioning of the rat basal forebrain leads to memory impairments in passive and active avoidance tasks.	effects of the bilateral electrolytic lesioning of the basal forebrain (bf), including the ventral globus pallidus, on passive or active avoidance tasks, were studied in male wistar rats. a severe deficit in acquisition of passive avoidance response was produced by the lesioning in the posterior level of bf. the retention of the passive avoidance response was markedly disrupted with post-training lesioning. time-dependent but only slight recovery from the memory impairments was observed in the p ...	1985	3971182
some problems associated with adenylate cyclase bioassays for parathyroid hormone.	a renal adenylate cyclase system was assessed for its suitability in the performance of parathyroid hormone (pth) bioassays. membrane preparations from 1 week old chicks were found to be more sensitive to pth than material from humans, dogs or rats. because of the presence of non-specific inhibitors and stimulators of adenylate cyclase in human serum, each serum sample was assayed in the presence and in the absence of a specific pth inhibitor and the difference was used to calculate pth activity ...	1985	3971661
effects of neuronal activity on inositol phospholipid metabolism in the rat autonomic nervous system.	the effect of nerve stimulation on inositol phospholipid hydrolysis in autonomic tissue was assessed by direct measurement of [3h]inositol phosphate production in ganglia that had been preincubated with [3h]inositol. within minutes, stimulation of the preganglionic nerve increased the [3h]inositol phosphate content of the superior cervical sympathetic ganglion indicating increased hydrolysis of inositol phospholipids. this effect was blocked in a low ca2+, high mg2+ medium. it was also greatly r ...	1985	3973589
[current insights into an old problem: bvd (bovine virus diarrhea)].	a number of recent papers on the epidemiology of bovine virus diarrhoea and the pathogenesis of mucosal disease resulted in new insights, but also gave rise to several questions. prior to dealing with these questions the new views are discussed, and the possible damaging effects of bvd infection are briefly reviewed.	1985	3975890
ultrastructural changes in the spleen of the natterjack, bufo calamita, after antigenic stimulation.	in the present study comparative aspects of the ultrastructure of the spleen were analyzed in non-immunized and t-dependent antigen-challenged natterjacks, bufo calamita. special attention is focused on the role of the non-lymphoid components in the splenic immunoreactivity. ten days after primary immunization with sheep erythrocytes, splenic lymphoid follicles increase considerably in number and size. by that time, lymphoblasts, medium and large lymphocytes abound in the periphery of the white ...	1985	3978698
subunits of bovine lutropin. hormonal and immunological activity after separation with reversed-phase high-performance liquid chromatography.	by means of reversed-phase high-performance liquid chromatography, we have fractionated bovine lutropin (lh) standard preparations. the highly purified niamdd-blh-4 was fractionated into two components, while the less pure nih-lh-b9 revealed three distinct peaks. the eluted material was further characterized by in vitro bioassay and by homologous radioimmunoassay for bovine lh, ovine lh-alpha and ovine lh-beta subunits. the material with the shortest retention time possessed almost no lh-activit ...	1985	3980634
evaluation of the micro-id system for the identification of yersinia pestis.	one hundred isolates of yersinia pestis identified by conventional means were tested by the micro-id system to assess its reliability for distinguishing y. pestis from other members of the family enterobacteriaceae. the micro-id system gave y. pestis as a choice for the identification of 89 of these cultures, although not always as the first choice. most nitrate-negative strains of y. pestis keyed out with yersinia pseudotuberculosis as first choice and y. pestis as second or fourth choice.	1985	3980695
metastatic nontraumatic clostridium septicum osteomyelitis.	nontraumatic clostridial infections are rare, but need to be diagnosed and treated early or high morbidity and mortality rates result. we believe that this is the first reported case of metastatic nontraumatic clostridium septicum osteomyelitis. early treatment with surgical debridement and parenteral antibiotics without hyperbaric oxygen was used. an associated occult rectal malignancy was discovered and treated.	1985	3980945
[chromatin activation and various problems of genetic activity regulation in eukaryotic cells].	in this paper the results of the investigation of activated chromatin are considered in terms of modern ideas of molecular mechanisms of genetic activity regulation. particular attention is given to the results obtained in the laboratory for functional morphology of chromosomes at the institute of molecular biology in 1973-1984.	1985	3982409
repression and inhibition of cephalosporin synthetases in streptomyces clavuligerus by inorganic phosphate.	cephalosporin production by growing cells of streptomyces clavuligerus was reduced by 100 mm inorganic phosphate. resting cell production was repressed by prior growth in high phosphate and inhibited by phosphate. the cell-free activity of desacetoxycephalosporin c synthetase (ring expansion activity) was repressed by prior growth in high phosphate and inhibited by phosphate. isopenicillin n synthetase (cyclase) was inhibited but not repressed. penicillin epimerase was neither inhibited nor repr ...	1985	3985747