Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| advances in the diagnosis and treatment of human papillomavirus infections. | during the past decade, much has been learned about the natural history of hpv. most infections occur early in one's sexual life. the overwhelming majority of infections are cleared by the host immune system and never present as warts or neoplasia. certain patient behavior such as smoking, frequent sex with many different partners, other std infections, especially hiv, and immune-suppressive drugs promote hpv expression and cause persistent infection. persistent hpv infection is very strongly re ... | 1999 | 10370842 |
| a matched prospective study of human immunodeficiency virus serostatus, human papillomavirus dna, and cervical lesions detected by cytology and colposcopy. | to compare the prevalence and type of human papillomavirus (hpv) infections in the genital tract of human-immunodeficiency-virus- (hiv) seropositive and -seronegative women matched for cytology and to examine prospectively the relationship of hpv dna, colposcopic findings and cervical squamous intraepithelial lesions (sil) in these matched seropositive and seronegative cohorts. | 1999 | 10371475 |
| activation of rna polymerase iii transcription in cells transformed by simian virus 40. | rna polymerase (pol) iii transcription is abnormally active in fibroblasts that have been transformed by simian virus 40 (sv40). this report presents evidence that two separate components of the general pol iii transcription apparatus, tfiiib and tfiiic2, are deregulated following sv40 transformation. tfiiic2 subunits are expressed at abnormally high levels in sv40-transformed cells, an effect which is observed at both protein and mrna levels. in untransformed fibroblasts, tfiiib is subject to r ... | 1999 | 10373542 |
| detection of human papilloma virus and epstein-barr virus dna in nasopharyngeal carcinoma by polymerase chain reaction. | nasopharyngeal carcinoma (npc) is an unique epithelial malignancy which occurs at a high frequency in certain regions of southeast asia. human papilloma virus (hpv) and epstein-barr virus (ebv) have both been identified in tissue specimens from npc. nevertheless, the association between viral infection and npc remains unclear. the purpose of this study was to demonstrate that simultaneous infection with ebv and hpv can occur in npc. eighty-eight fresh tissue samples which contained sufficient an ... | 1999 | 10375867 |
| evaluation of the risk of cervical intraepithelial neoplasia and human papilloma virus infection in renal transplant patients receiving immunosuppressive therapy. | to investigate the risk of cervical intraepithelial neoplasia and the coexistence of human papilloma virus (hpv) infection in renal transplant patients receiving immunosuppressive therapy. | 1999 | 10376431 |
| the nature of the attenuation of salmonella typhimurium strains expressing human papillomavirus type 16 virus-like particles determines the systemic and mucosal antibody responses in nasally immunized mice. | we have recently shown by using a recombinant salmonella typhimurium phopc strain in mice the feasibility of using a salmonella-based vaccine to prevent infection by the genital human papillomavirus type 16 (hpv16). here, we compare the hpv16-specific antibody responses elicited by nasal immunization with recombinant s. typhimurium strains harboring attenuations that, in contrast to phopc, are suitable for human use. for this purpose, chi4989 (deltacya deltacrp) and chi4990 [deltacya delta(crp-c ... | 1999 | 10377159 |
| [transformation of immortalized rat embryonic fibroblasts by the e7 gene of human type 16 papillomavirus]. | 1999 | 10377575 | |
| rapid dna mutation identification and fingerprinting using base excision sequence scanning. | base excision sequence scanning (bess) is a new polymerase chain reaction (pcr)-based mutation scanning method that locates and identifies all dna mutations. the bess method consists of two procedures that generate "t" (bess t-scan) and "g" ladders (bess g-tracker) analogous to t and g ladders of dideoxy sequencing. the bess procedures are simple to perform and require no special equipment or gels, no reaction optimization beyond pcr, and no heteroduplex formation. the samples are analyzed on st ... | 1999 | 10380756 |
| implication of p53 in growth arrest and apoptosis induced by the synthetic retinoid cd437 in human lung cancer cells. | cd437 is a novel retinoid that can induce apoptosis in a variety of tumor cell types by an unknown mechanism. we found that cd437 up-regulated the expression of p21(waf1/cip1), bax, and killer/dr5 and induced g1 arrest and rapid apoptosis in three human non-small cell lung carcinoma cell lines with wild-type p53 but not in five cell lines with mutant p53, suggesting a role for p53 in the effects of cd437. using h460 cells in which wild-type p53 protein was degraded by transfection of the human p ... | 1999 | 10383141 |
| p16/prb pathway alterations are required for bypassing senescence in human prostate epithelial cells. | the cell cycle regulatory genes p16/cdkn2 and rb are frequently deleted in prostate cancers. in this study, we examined the role of alterations in p16 and prb during growth, senescence, and immortalization in vitro of human prostate epithelial cells (hpecs). hpecs are established from normal prostate tissues and cultured on collagen-coated dishes. our results show that p16 is reproducibly elevated at senescence in hpecs. hpecs are immortalized using human papilloma virus 16 e6 and/or e7 as molec ... | 1999 | 10383161 |
| the human papillomavirus type 11 upstream regulatory region triggers hair-follicle-specific gene expression in transgenic mice. | we have generated transgenic mice carrying the urr of the human papillomavirus type 11 ligated in front of the escherichia coli beta-galactosidase coding region sequence. using x-gal staining to demonstrate beta-galactosidase production, we observed a hair-specific transcription of the reporter gene. this transcription was limited to the epithelial cells of the hair bulge region. the transgene was developmentally regulated, as no lacz staining was demonstrated during embryogenesis and specific s ... | 1999 | 10383735 |
| squamous cell carcinoma of the vulva in brazil: prognostic importance of host and viral variables. | certain clinicopathologic features of vulvar squamous cell carcinoma have been correlated with adverse prognosis. however, few large-scale studies have addressed their role in patient survival. this study examined the relationship between multiple variables and prognosis in a large group of vulvar cancers in brazil. | 1999 | 10385552 |
| the human papillomavirus e7 oncoprotein abrogates signaling mediated by interferon-alpha. | greater than 95% of all cervical carcinomas have been found to be associated with "high-risk" human papillomavirus (mainly types 16 and 18) infections, with the viral e6 and e7 oncoproteins essential for neoplastic development and maintenance. interferon-alpha (ifnalpha) is used in the treatment of hpv infections yet both in vivo and in vitro data suggest that the virus has developed mechanisms to avoid the effects of interferon. here we show that the hpv16 e7 oncoprotein is able to inhibit the ... | 1999 | 10388655 |
| uniform distribution of hpv 16 e6 and e7 variants in patients with normal histology, cervical intra-epithelial neoplasia and cervical cancer. | high-risk human papillomaviruses (hpv), particularly hpv 16, are associated with invasive cervical cancer (icc), and persistent high-risk hpv infection is considered to be a marker for progressive cervical intra-epithelial neoplasia (cin). however, most high-risk, hpv-infected, pre-cancerous lesions will not progress to invasion. several reports suggest that specific hpv 16 e6 and/or e7 sequence variations may be associated with a high risk for progression. no data from german patients have so f ... | 1999 | 10389753 |
| cloning of e6 and e7 genes of human papilloma virus type 18 and transformation potential of e7 gene and its mutants. | e6 and e7 genes of human papilloma virus type 18 have been subcloned from plasmid pc7, carrying an insert of dna from squamous cell carcinoma of cervix. both genes in comparison to prototype variant contain one mutation that changes asparagine to leucine. in the case of e6 gene this mutation is mapped in codon 129, in the case of e7 the same change aac to aaa mapped in codon 92. in addition both genes contain few point mutations that do not change the aminoacid sequences of the protein. two muta ... | 1999 | 10403700 |
| sialadenoma papilliferum of the palate: case report and literature review. | sialadenoma papilliferum (sp) is a rare tumor of salivary gland ducts which bears a strong histologic resemblance to the more common syringocystadenoma papilliferum (scap). we report a case occurring on the palate of a 50-year-old man, and review the clinical and histologic features of this tumor. because of the histologic similarities between these two tumors and squamous papillomas, polymerase chain reaction (pcr) for human papilloma virus (hpv) dna was performed on this tumor and on two cases ... | 1999 | 10408352 |
| screening for genital human papillomavirus: results from an international validation study on human papillomavirus sampling techniques. | the objective of this study was to determine the validity of human papillomavirus (hpv) detection using exfoliated cervical cells compared with cervical biopsy specimens in women with normal cervix and to assess whether hpv detection rates using exfoliated cells is dependent on the number and order in which cervical scrapes are taken. women undergoing hysterectomy for reasons other than cervical cancer were recruited in three hospitals in countries with varying risks of cervical cancer. after in ... | 1999 | 10408790 |
| human papillomavirus infection and other risk factors for cervical intraepithelial neoplasia in japan. | various risk factors were investigated in 167 cervical intra-epithelial neoplasia (cin) case and control pairs in japan. cin cases showed evidence of nine known risk factors including smoking and sexual behaviour. however, after adjustment for papillomavirus infection, the highest determinant, the only remaining risk factors were: being married, early age at first pregnancy and multiparity. | 1999 | 10408876 |
| establishment of an immortalized fetal intrapulmonary artery endothelial cell line. | the investigation of fetal pulmonary endothelial cell gene expression and function has been limited by the requirement for primary cells. in an effort to establish an immortalized cell line, ovine fetal pulmonary artery endothelial cells (paecs; passage 5) were permanently transfected with the e6 and e7 open reading frames of human papillomavirus type 16, and phenotypes related to nitric oxide (no) production were evaluated up to passage 28. acetylated low-density lipoprotein uptake, endothelial ... | 1999 | 10409236 |
| clinical, histopathologic, and molecular aspects of cutaneous human papillomavirus infections. | human papillomaviruses comprise a large family of double stranded dna viruses that are the etiologic agents of benign warts and anogenital cancers. at least 82 different human papillomavirus types have been identified and many remain yet uncharacterized. the development of new molecular techniques in recent years has led to an increased understanding of human papillomaviruses and their roles in carcinogenesis. several clinicopathologic entities arising from human papillomavirus infection encount ... | 1999 | 10410856 |
| human papillomavirus 18 oncoproteins e6 and e7 enhance irradiation- and chemotherapeutic agent-induced apoptosis in p53 and rb mutated cervical cancer cell lines. | tumor suppressor genes p53 and rb are important in cell cycle control. necessity of wild type p53 is implicated in irradiation-induced apoptosis. numerous tumor cells carry p53 mutations and yet are sensitive to irradiation or chemotherapeutic agents. therefore p53-and rb-independent pathways must exist to account for irradiation-induced apoptosis. we evaluated the apoptotic response of a p53- and rb-mutated, human papilloma virus (hpv) negative cervical cancer cell line (c33a), and c33a cell li ... | 1999 | 10410876 |
| genital bowen disease associated with an unusual human papillomavirus type 57b. | 1999 | 10411173 | |
| perianal bowen's disease and anal intraepithelial neoplasia: review of the literature. | the aim of this study was to review the literature with regard to perianal bowen's disease and anal intraepithelial neoplasia. | 1999 | 10411443 |
| human papillomavirus type 31 oncoproteins e6 and e7 are required for the maintenance of episomes during the viral life cycle in normal human keratinocytes. | the e6 and e7 oncoproteins of the high-risk human papillomavirus (hpv) types are able to immortalize human keratinocytes in vitro and likely contribute to the development of anogenital malignancies in vivo. the role of these oncoproteins in the productive viral life cycle, however, is not known. to begin to examine these possible roles, mutations in e6 were introduced in the context of the complete hpv 31 genome. although transfected wild-type hpv 31 genomes, as well as genomes containing an e6 ... | 1999 | 10411895 |
| short-term follow up of cervical squamous intraepithelial lesions associated with hiv and human papillomavirus infections in africa. | a prospective study in gynaecology clinics was conducted in abidjan, côte d'ivoire, to assess the short-term evolution of squamous intraepithelial lesions (sils). of 94 women with a cytological diagnosis of sil, 38 were infected with hiv. the average follow-up period after the initial smear was 5 months. detection of human papillomavirus (hpv) by polymerase chain reaction (pcr) was performed at both the time of enrolment and final follow-up smear. there were 39 cases of persistent sils. hiv-posi ... | 1999 | 10414878 |
| does plantar epidermoid cyst with human papillomavirus infection originate from the eccrine dermal duct? | 1999 | 10417539 | |
| mucosal oncogenic human papillomaviruses and extragenital bowen disease. | genital bowen disease is known to have a strong association with human papillomavirus (hpv) type 16. on the other hand, previous studies of extragenital bowen disease (ebd) that have used different hybridization techniques have produced discordant results in the detection of mucosal oncogenic hpv. | 1999 | 10421264 |
| pleiotropic effects of human papillomavirus type 16 e6 oncogene expression in human epithelial cell lines. | two human papillomavirus (hpv)-negative epithelial cell lines, hacat and c33a, were transfected with hpv-16 e6 and analysed for functional consequences which are relevant to invasive tumour progression. after transfection with e6, both cell lines invaded collagen matrices, in contrast to vector-transfected control cells. the e6-expressing cells showed a marked increase in expression of the beta1 integrin subunit, with no or relatively minor alterations in the levels of a range of integrin subuni ... | 1999 | 10423139 |
| collection devices for human papillomavirus. | human papillomavirus (hpv) testing has relied to date on samples collected by experienced health professionals. self-administered testing devices could allow hpv testing to occur in large-scale epidemiologic studies of primary care screening populations. the purpose of this study is to determine whether a self-collection device for cervicovaginal hpv infection could be developed. | 1999 | 10428251 |
| colposcopy in pregnancy: directed brush cytology compared with cervical biopsy. | to evaluate colposcopically directed brush cytology as a substitute for directed biopsy of acetowhite lesions identified during pregnancy. | 1999 | 10432127 |
| oncological complications of human immunodeficiency virus disease and hematologic consequences of their treatment. | despite advances in antiretroviral therapy and in the treatment and prevention of opportunistic infections, oncological and consequent hematologic complications of human immunodeficiency virus (hiv) infection continue to occur and are of significant clinical importance. virus-related tumors (e.g., kaposi's sarcoma, induced by human herpesvirus 8; non-hodgkin's lymphoma, linked to epstein-barr virus; and anogenital tumors, linked to human papillomavirus) are frequent in patients with hiv-induced ... | 1999 | 10433562 |
| mdm2 binds p73 alpha without targeting degradation. | the function of the p53 tumor suppressor protein is regulated by interaction with mdm2, which targets p53 for ubiquitin dependent degradation. we show here that like p53, p73 alpha forms an interaction with mdm2, both in vitro and in cells, but this does not result in the degradation of the p73 alpha protein. the human papillomavirus e6 protein also fails to degrade p73 alpha, suggesting that the mechanisms governing p73 alpha stability are distinct from those known to regulate p53 stability. ho ... | 1999 | 10435614 |
| expression of human papillomavirus 16 e2 protein in schizosaccharomyces pombe delays the initiation of mitosis. | infection by some types of human papillomavirus (hpv) is associated with the development of cervical cancer. analysis of viral dna from cervical tumours shows that the e2 gene is frequently disrupted during integration into the host cell's dna. it has therefore been suggested that loss of e2p is an important step in malignant transformation. expression of e2p in the fission yeast schizosaccharomyces pombe retards the g2-m transition, by delaying activation of cdc2p kinase. in contrast, s phase p ... | 1999 | 10435625 |
| regulation of human papillomavirus type 31 polyadenylation during the differentiation-dependent life cycle. | the l1 and l2 capsid genes of human papillomavirus type 31 (hpv-31) are expressed late in the differentiation-dependent life cycle from a promoter located in the e7 open reading frame (orf) of the early region. these late hpv genes are transcribed by rna polymerase ii which reads through the region containing early polyadenylation signals and proceeds to a poly(a) site downstream of l1. in this study, we have investigated the mechanisms regulating differentiation-dependent polyadenylation and re ... | 1999 | 10438805 |
| human papillomavirus type 16 e7 dna vaccine: mutation in the open reading frame of e7 enhances specific cytotoxic t-lymphocyte induction and antitumor activity. | a human papillomavirus type 16 e7 dna vaccine with the open reading frame encoding mutations in two zinc-binding motifs expressed a rapidly degraded e7 protein. this vaccine induced a significantly stronger e7-specific cytotoxic t-lymphocyte response and better tumor protection in mice than did a wild-type e7 dna vaccine expressing a stable e7 protein. | 1999 | 10438884 |
| nuclear import of hpv11 l1 capsid protein is mediated by karyopherin alpha2beta1 heterodimers. | l1 major capsid proteins of human papillomaviruses (hpvs) enter the nuclei of host cells at two times during the viral life cycle: 1) after infection and 2) later during the productive phase, when they assemble the replicated hpv genomic dna into infectious virions. l1 proteins are stable in two oligomeric configurations: as homopentameric capsomers, and as capsids composed of 72 capsomers. we found that intact l1 capsids of hpv type 11 cannot enter the nucleus, suggesting that capsid disassembl ... | 1999 | 10440932 |
| new concepts on the role of human papillomavirus in cell cycle regulation. | human papillomaviruses (hpvs) are strictly host-specific and also show a distinct tropism to squamous epithelial cells. upon hpv infection, only a portion of the virus reaching the nucleus seems to undergo replication, suggesting that hpv replication remains confined to a small number of cells. hpvs critically depend on the cellular machinery for the replication of their genome. viral replication is restricted to differentiated keratinocytes that are normally growth arrested. hence, hpvs have de ... | 1999 | 10442672 |
| [detection of human papillomavirus types in tumors and inflammations of the eye]. | infections by the human papillomavirus occur in the skin and in the mucosa. they may play a significant role in the neoplastic transformation of cells. at least half of all the epithelial tumours of the ocular surface and the lacrimal drainage system have been shown to be hpv associated. however, in ca. 50% of these tumors only a limited number of mucosal hpv types could be detected by commercially available test systems. this study reports of hpv specific sequences in putatively hpv associated ... | 1999 | 10448632 |
| human papillomavirus is a necessary cause of invasive cervical cancer worldwide. | a recent report that 93 per cent of invasive cervical cancers worldwide contain human papillomavirus (hpv) may be an underestimate, due to sample inadequacy or integration events affecting the hpv l1 gene, which is the target of the polymerase chain reaction (pcr)-based test which was used. the formerly hpv-negative cases from this study have therefore been reanalyzed for hpv serum antibodies and hpv dna. serology for hpv 16 vlps, e6, and e7 antibodies was performed on 49 of the 66 cases which w ... | 1999 | 10451482 |
| loss of retinoblastoma protein expression is frequent in small cell neuroendocrine carcinoma of the cervix and is unrelated to hpv type. | we have previously identified an inverse relationship between p53 and retinoblastoma protein (prb) immunoreactivity in non-small cell carcinoma of the cervix. because prb is infrequently expressed in small cell carcinoma of the lung, we analyzed 25 small cell neuroendocrine carcinomas of the cervix to test the hypotheses that 1) lack of prb expression is associated with the neuroendocrine phenotype in human papillomavirus (hpv)-associated cervical carcinoma and 2) the inverse relationship betwee ... | 1999 | 10452502 |
| the g(2) checkpoint is maintained by redundant pathways. | while p53 activity is critical for a dna damage-induced g(1) checkpoint, its role in the g(2) checkpoint has not been compelling because cells lacking p53 retain the ability to arrest in g(2) following dna damage. comparison between normal human foreskin fibroblasts (hffs) and hffs in which p53 was eliminated by transduction with human papillomavirus type 16 e6 showed that treatment with adriamycin initiated arrest in g(2) with active cyclin b/cdc2 kinase, regardless of p53 status. both e6-trans ... | 1999 | 10454534 |
| genetic susceptibility to hpv infection and cervical cancer. | squamous cell carcinoma of the cervix (sccc) is one of the leading causes of death in developing countries. infection with high-risk human papillomavirus (hpv) is the major risk factor to develop malignant lesions in the cervix. polymorphisms of the mhc and p53 genes seem to influence the outcome of hpv infection and progression to sccc, although controversial data have been reported. mhc are highly polymorphic genes that encode molecules involved in antigen presentation, playing a key role in i ... | 1999 | 10454752 |
| the scymv1 hairpin ribozyme: targeting rules and cleavage of heterologous rna. | the catalytic center of the rna from the negative strand of the satellite rna of chicory yellow mottle virus type 1 (scymv1) is in the hairpin ribozyme family, has catalytic activity, and cleaves substrates before a preferred gua sequence. this is different from that of the satellite rna from the negative strand of tobacco ringspot virus (strsv) which prefers a guc sequence at the site of cleavage. the scymv1 hairpin ribozyme has now been developed for cleaving heterologous rna substrates. when ... | 1999 | 10455414 |
| cellular transcription factors regulate human papillomavirus type 16 gene expression by binding to a subset of the dna sequences recognized by the viral e2 protein. | human papillomavirus type 16 (hpv-16) is a dna tumour virus that has been implicated in the development of cervical cancer. the hpv-16 e2 protein binds to four sites that are present upstream of the viral p97 promoter and regulates transcription of the e6 and e7 oncogenes. here, it is shown that cellular transcription factors bind to two of these e2 sites. one cellular e2 site-binding factor, which is here named cef-1, binds tightly to e2 site 1. cef-2, an unrelated cellular e2 site-binding fact ... | 1999 | 10466807 |
| papillomavirus e2 induces p53-independent apoptosis in hela cells. | we have previously shown that expression of the papillomavirus e2 protein in hela cells induces p53 accumulation and causes both cell cycle arrest and apoptosis. in contrast to growth arrest, onset of apoptosis was not correlated with an increase of p53 transcriptional activity. in the present study, we conducted biochemical and genetic experiments in order to determine whether e2-induced apoptosis was independent of p53 induction. we showed that e2 did not alter the transcription of bax, a know ... | 1999 | 10467398 |
| induction of cellular immunosuppression by the human papillomavirus type 16 e7 oncogenic protein. | the human papillomavirus type 16 (hpv-16) e7 oncogenic protein is found in the culture supernatant of siha cells, a cervical carcinoma cell line. extracellular e7 protein, acting as a viral toxin in human immune cells, induces the overproduction of the immune suppressive ifn alpha cytokine by apcs, and inhibits the t-cell response to recall and allogenic antigens. these effects should be taken into account for the design of anti-human cervical carcinoma vaccines. | 1999 | 10472433 |
| th1 cytokine patterns in cervical human papillomavirus infection. | the host's immune response to cervical human papillomavirus (hpv) infection is poorly understood. in a longitudinal cohort of women with cervical hpv infections, defined by pcr-based hpv dna testing, we used exfoliated cervical cells and reverse transcription-pcr to examine the cervical mucosal mrna expression of cytokines involved in regulating cell-mediated immunity. we identified seven hpv-positive subjects who were found to have cleared their hpv infections 4 months later. in all seven, a t- ... | 1999 | 10473530 |
| characterization of the regulatory domains of the human skn-1a/epoc-1/oct-11 pou transcription factor. | the skn-1a pou transcription factor is primarily expressed in keratinocytes of murine embryonic and adult epidermis. although some pou factors expressed in a tissue-specific manner are important for normal differentiation, the biological function of skn-1a remains unknown. previous in vitro studies indicate that skn-1a has the ability to transactivate markers of keratinocyte differentiation. in this study, we have characterized skn-1a's transactivation domain(s) and engineered a dominant negativ ... | 1999 | 10473598 |
| characterization of immortalized rabbit lacrimal gland epithelial cells. | to establish an immortalized lacrimal gland epithelial cell line, the orbital lacrimal glands of normal new zealand white rabbits were multiply injected with an immortalizing amphotropic retroviral vector (lxsn16e6e7) containing the e6 and e7 genes of human papillomavirus type 16. lacrimal glands were removed after 2 d and acinar epithelial cells were isolated and cultured on matrigel-coated 60 mm2 plates containing dmem-f12 supplemented with 5% nu-serum v. transformed cells were selected in g41 ... | 1999 | 10478799 |
| allelic losses at chromosome 3p are seen in human papilloma virus 16 associated transitional cell carcinoma of the cervix. | transitional cell carcinomas (tccs) of the cervix are rare neoplasms of the female genital tract. although these tumors display urothelial differentiation, there is controversy regarding their histogenetic relationship to squamous cell carcinomas (scc) of the cervix versus transitional cell carcinomas of the bladder. | 1999 | 10479494 |
| [human papillomavirus oncogenes and immortalization of cultured human cells]. | 1999 | 10483302 | |
| human papillomavirus and prognoses of patients with cancers of the upper aerodigestive tract. | some studies have shown that human papillomavirus (hpv) infection may play not only an etiologic role in anogenital cancers but also a role in the clinical outcome. the objective of the current study was to determine whether detection of hpv dna in primary squamous cell carcinomas of the upper aerodigestive tract (uadt) is a prognostic factor in patients with the disease. | 1999 | 10223228 |
| interlaboratory agreement of different human papillomavirus dna detection and typing assays in cervical scrapes. | 1999 | 10225460 | |
| prevalence of human papilloma virus dna in head and neck cancers carrying wild-type or mutant p53 tumor suppressor genes. | the normal function of the p53 tumor suppressor protein can be perturbed by non-mutational mechanisms. the e6 protein encoded by high risk strains of human papilloma virus (hpv) targets the p53 protein resulting in enhanced degradation via the ubiquitin pathway. we have used nested pcr for detecting the presence of hpv dna in 58 primary head and neck tumors and 15 metastatic lymph nodes, which had been prescreened for p53 mutations in exons 5 to 8. hpv dna sequences were detected in 12 tumors (2 ... | 1999 | 10226517 |
| chimeric papillomavirus virus-like particles induce a murine self-antigen-specific protective and therapeutic antitumor immune response. | the use of chimeric virus-like particles represents a new strategy for delivering tumor antigens to the immune system for the initiation of antitumor immune responses. immunization of dba/2 mice with the p1a peptide derived from the p815 tumor-associated antigen p1a induced specific t-cell tolerance, resulting in progression of a regressor p815 cell line in all animals. however, immunization with a human papillomavirus type 16 l1 virus-like particle containing the p1a peptide in the absence of a ... | 1999 | 10227378 |
| human papillomavirus types 16 and 39 in a vulval carcinoma occurring in a woman with hailey-hailey disease. | a woman with hailey-hailey disease, suffering from carcinoma of the vulva, was examined by histology and for the presence of human papillomavirus (hpv) dna by polymerase chain reaction (pcr) and in situ hybridization. our diagnosis by histological examination revealed the vulval carcinoma to be a squamous cell carcinoma (scc), adjacent to lesions of hailey-hailey disease and severe dysplasia/carcinoma in situ [vulval intraepithelial neoplasia (vin) iii]. the pcr with consensus primers for the l1 ... | 1999 | 10233276 |
| allelic loss in human papillomavirus-positive and -negative vulvar squamous cell carcinomas. | vulvar squamous cell carcinoma (vscc) is a biologically and morphologically diverse disease, consisting of human papillomavirus (hpv)-positive and -negative tumors that differ in their morphological phenotypes and associated vulvar mucosal disorders. this study analyzed the frequencies of allelic loss (loss of heterozygosity (loh)) in hpv-positive and -negative vsccs to identify potential targets for the study of preinvasive diseases, to determine whether hpv status influenced patterns of loh, a ... | 1999 | 10233839 |
| characterization of a major neutralizing epitope on human papillomavirus type 16 l1. | persistent infection with human papillomavirus type 16 (hpv-16) is strongly associated with the development of cervical cancer. neutralizing epitopes present on the major coat protein, l1, have not been well characterized, although three neutralizing monoclonal antibodies (mabs) had been identified by using hpv-16 pseudovirions (r. b. roden et al., j. virol. 71:6247-6252, 1997). here, two of these mabs (h16.v5 and h16.e70) were demonstrated to neutralize authentic hpv-16 in vitro, while the thir ... | 1999 | 10233949 |
| trichostatin a up-regulates human papillomavirus type 11 upstream regulatory region-e6 promoter activity in undifferentiated primary human keratinocytes. | human papillomavirus (hpv) gene expression in squamous epithelia is differentiation dependent in benign patient lesions and in organotypic raft cultures of primary human keratinocytes (phks). using the lacz reporter in raft cultures, we previously showed that this transcriptional regulation of the hpv type 11 (hpv-11) enhancer-promoter located in the upstream regulatory region (urr) appears to have resulted from coordination between the transcription transactivators ap1, oct1, and sp1 in differe ... | 1999 | 10233965 |
| identifying women with cervical neoplasia: using human papillomavirus dna testing for equivocal papanicolaou results. | a papanicolaou (pap) test result of atypical squamous cells of undetermined significance (ascus) presents a clinical challenge. only 5% to 10% of women with ascus harbor serious cervical disease, but more than one third of the high-grade squamous intraepithelial lesions (hsils) in screening populations are identified from ascus pap test results. | 1999 | 10235153 |
| resolution of recalcitrant hand warts in an hiv-infected patient treated with potent antiretroviral therapy. | human papilloma virus (hpv)-related cutaneous manifestations occur with increased frequency and severity among hiv-infected persons. in this report, we describe an hiv-infected man with persistent, severe cutaneous hand warts that did not respond to multiple therapies, including liquid nitrogen cryotherapy, topical dinitrochlorobenzene, topical podophyllin, and intralesional interferon-alfa injections. approximately 1 year after starting a potent protease inhibitor-containing antiretroviral regi ... | 1999 | 10321624 |
| induction of the p53-target gene gadd45 in hpv-positive cancer cells. | the e6 oncoprotein of human papillomaviruses (hpvs) has the potential to functionally antagonize p53. in several experimental model systems, ectopic expression of e6 can block the genotoxic induction of the growth inhibitory p53 target gene gadd45, suggesting that the inactivation of this pathway may play a major role for hpv-associated cell transformation. here, we investigated whether this reflects the regulation of gadd45 expression in carcinoma-derived hpv-positive cells. we found that the g ... | 1999 | 10327059 |
| is human papillomavirus testing of value in clinical practice? | the objective of this review was to evaluate the current status of human papillomavirus testing in predicting the presence of high-grade or invasive disease in the cervix in women with papanicolaou smears reported as atypical squamous cells of undetermined significance (ascus) or low-grade squamous intraepithelial lesion. this is a review of many of the studies already published evaluating the utility of human papillomavirus testing as part of the triage for patients whose papanicolaou smears we ... | 1999 | 10329854 |
| cervical human papillomavirus deoxyribonucleic acid persists throughout pregnancy and decreases in the postpartum period. | our goal was to determine the persistence of human papillomavirus infection of the cervix in a prospectively evaluated cohort of pregnant women observed from the first trimester until after delivery. | 1999 | 10329863 |
| target site selection for an rna-cleaving catalytic dna. | a small catalytic dna, known as the 10-23 dna enzyme or deoxyribozyme, has been shown to efficiently hydrolyze rna at purine-pyrimidine (r-y) junctions in vitro. although these potentially cleavable junctions are ubiquitous, they are often protected from deoxyribozyme activity by rna secondary structure. we have developed a multiplex cleavage assay for screening the entire length of a target rna molecule for deoxyribozyme cleavage sites that are efficient, both in terms of kinetics and accessibi ... | 1999 | 10331809 |
| hpv in situ hybridization with catalyzed signal amplification and polymerase chain reaction in establishing cerebellar metastasis of a cervical carcinoma. | we report an unusual case of cerebellar metastasis from a cervical adenosquamous carcinoma in which molecular techniques assisted in establishing the correct diagnosis. the patient was a 43-year-old woman with surgically unresectable cervical carcinoma diagnosed 2 years before presenting with neurological symptoms. a magnetic resonance imaging scan showed a large, enhancing cerebellar lesion with significant brain stem compression. the excised cerebellar tumor resembled a small cell carcinoma an ... | 1999 | 10333232 |
| thin-layer cytology meets viral hybrid capture. | this commentary addresses the benefits of liquid-based thin-layer cervical cytology, and the applicability of residual samples for human papilloma virus assay. the potential for use of this technology in wide-scale screening as well as the many unanswered issues, in particular cost-benefit ratio and financing, are also discussed. | 1999 | 10342014 |
| a survey of seroprevalence of human papillomavirus types 16, 18 and 33 among children. | the importance and natural history of hpv infections in childhood is incompletely understood. we performed a survey for presence of serum antibodies to hpv capsids among 1031 children aged 0 to 13 years, resident in stockholm, sweden. the hpv seroprevalence among these children was 3.0% for hpv16, 0.6% for hpv18 and 2.7% for hpv33. by comparison, among simultaneously analyzed positive control panels comprising women with cin or healthy women with type-specific cervical hpv dna, seroprevalence of ... | 1999 | 9935145 |
| hpv 16 antibody prevalence in jamaica and the united states reflects differences in cervical cancer rates. | human papillomavirus (hpv) is widely accepted as the primary etiologic agent in the development of cervical cancer. dna of a particular hpv type, hpv 16, is found in about half of tumors tested. inconsistent with this causal relationship, however, population-based studies of hpv dna prevalence have often failed to find high rates of anogenital hpv infection in countries with high cervical cancer rates. to examine this issue, we used serology to compare hpv 16 exposure in healthy volunteer blood ... | 1999 | 9935171 |
| the chromatin structure of the long control region of human papillomavirus type 16 represses viral oncoprotein expression. | the long control region (lcr) of human papillomavirus type 16 (hpv-16) has a size of 850 bp (about 12% of the viral genome) and regulates transcription and replication of the viral dna. the 5' segment of the lcr contains transcription termination signals and a nuclear matrix attachment region, the central segment contains an epithelial cell-specific enhancer, and the 3' segment contains the replication origin and the e6 promoter. here we report observations on the chromatin organization of this ... | 1999 | 9971771 |
| detection and quantitation of human papillomavirus by using the fluorescent 5' exonuclease assay. | a method for the detection and quantitation of oncogenic human papillomavirus (hpv) was developed by using the fluorescent 5' exonuclease assay. the method is based on the amplification of a 180-bp fragment from the 3' part of the e1 open reading frame in a single pcr with type-specific probes for hpv types 16, 18, 31, 33, and 35. the probes can be used separately or in combinations of up to three probes per assay. quantitation over a range of 10(1) to 10(6) initial hpv copies was possible by us ... | 1999 | 9986801 |
| differential gene expression in neoplastic and human papillomavirus-immortalized oral keratinocytes. | we have previously demonstrated that normal human oral keratinocytes immortalized by transfection with human papillomavirus type-16 dna became tumorigenic after exposure to a chemical carcinogen. in an effort to detect differentially regulated genes associated with this transition from the immortal to the malignant phenotype, we employed representational differences analysis (a pcr-coupled subtractive hybridization technique). after analysing 50 colonies, 12 putative messages were identified. no ... | 1999 | 9989835 |
| laser conization of cervical intraepithelial neoplasia grade 3: free resection margins indicative of lesion-free survival. | various grades of cervical intraepithelial neoplasia may occur following laser conization for grade 3 lesions. the aim of this study was to assess lesion-free survival after laser conization in cases with/without free resection margins, and to test whether detection of human papillomavirus infection and/or p53 expression in the cone lesion were useful predictors of lesion-free survival. | 1999 | 9926893 |
| photodynamic therapy sensitivity is not altered in human tumor cells after abrogation of p53 function. | photodynamic therapy (pdt) is an effective local cancer treatment that induces cytotoxicity through the intracellular generation of reactive oxygen species. the current study investigated whether abrogation of wild-type p53 expression modified the sensitivity of tumor cells to pdt-mediated oxidative stress. in these experiments, human colon (ls513) and breast (mcf-7) carcinoma cells exhibiting a wild-type p53 phenotype were directly compared to ls513 and mcf-7 cells with abrogated p53 function i ... | 1999 | 9927042 |
| modulation of type m2 pyruvate kinase activity by the human papillomavirus type 16 e7 oncoprotein. | we report here that the e7 oncoprotein encoded by the oncogenic human papillomavirus (hpv) type 16 binds to the glycolytic enzyme type m2 pyruvate kinase (m2-pk). m2-pk occurs in a tetrameric form with a high affinity to its substrate phosphoenolpyruvate and a dimeric form with a low affinity to phosphoenolpyruvate, and the transition between both conformations regulates the glycolytic flux in tumor cells. the glycolytic intermediate fructose 1, 6-bisphosphate induces the reassociation of the di ... | 1999 | 9990017 |
| expression of the e6 and e7 genes of human papillomavirus (hpv16) extends the life span of human myoblasts. | primary human myoblasts (satellite cells), like other human cells, have a limited life span in vitro. here we show that expression of the e6e7 early region from human papillomavirus type 16 can greatly extend the life span of both fetal and satellite cell-derived myoblasts and release them from dependence on the growth factors normally necessary for their proliferation. expression of either the e6 or the e7 gene alone was not sufficient to confer this phenotype, although expression of e7 did del ... | 1999 | 10094825 |
| dna replication specificity and functional e2 interaction of the e1 proteins of human papillomavirus types 1a and 18 are determined by their carboxyl-terminal halves. | replication of most papillomaviruses (pvs) requires the viral-encoded e1 and e2 proteins that bind to the origin of replication (ori) containing the e1- and e2-binding sites and help recruit host replication factors during the initiation of dna replication. we studied the ability of heterologous e1 and e2 proteins to interact in vivo and support replication, using the human papillomavirus (hpv) types 1a and 18 as model systems. the e1 protein of hpv-1a in combination with hpv-18 e2 supported hig ... | 1999 | 10191198 |
| ccaat displacement protein binds to and negatively regulates human papillomavirus type 6 e6, e7, and e1 promoters. | expression of human papillomavirus genes increases as the target cell, the keratinocyte, differentiates. ccaat displacement protein (cdp) is a cellular protein which has been shown in other cell types to negatively regulate gene expression in undifferentiated cells but not in differentiated cells. we have previously shown that a 66-bp purine-thymidine-rich sequence (the 66-mer) binds cdp and negatively regulates the human papillomavirus type 6 (hpv-6) e6 promoter (s. pattison, d. g. skalnik, and ... | 1999 | 10196318 |
| [research on the oncogenesis of cervical epithelial cells co-induced by human papillomavirus and human cytomegalovirus]. | the carcinogenesis of the human cervical carcinoma is known closely associated with human papillomavirus (hpv). the purpose of this article is to identify whether another sexually transmitted factor, human cytomegalovirus (hcmv) plays as a co-factor role in the carcinogenesis. | 1999 | 11869507 |
| [effects of human papillomavirus infection on pregnant women and chorionic trophoblasts]. | to evaluate the effects of human papilloma virus (hpv) infection on pregnant women and chorionic trophoblasts. | 1999 | 11360592 |
| [relationship between histopathologic observation of cervical condyloma and human papillomavirus infection]. | to study the relationship between histopathologic changes of cervical condyloma and different subtypes human papillomavirus (hpv) infection. | 1999 | 11360614 |
| lack of human papillomavirus types 16 and 18 dna in esophageal squamous carcinoma cell lines. | to assess the role of human papillomavirus (hpv) in the carcinogenesis of the esophageal carcinomas in a high-incidence geographical area in northern china. | 1999 | 11601333 |
| clinicians should check women with hpv for hiv. | studies presented at the 6th conference on retroviruses and opportunistic infections indicate that women with genital ulcers or sexually transmitted diseases such as human papilloma virus (hpv) should be tested for hiv. genital ulcers could provide a portal of entry for the virus, as well as help transmit the virus to others. appropriate treatment and sexual abstinence are recommended to prevent the spread of hiv and genital ulcers, which are most often caused by herpes or syphilis. another grou ... | 1999 | 11366219 |
| annual colposcopies and pap smears recommended for women with hiv. | a new study presented by annekathryn goodman of massachusetts general hospital indicates that hiv positive women should receive annual colposcopies, along with pap smears, to detect abnormal cell growth early. the recommendation is due to the fact that hiv positive women are more likely to have false-negative pap smears than hiv negative women. in a related development, the fda approved a new dna-based blood test to detect human papillomavirus, which is associated with cervical and anal cancer. ... | 1999 | 11366700 |
| hiv transmission. | a presentation at the 39th interscience conference on antimicrobial agents and chemotherapy (icaac) noted that people with genital warts have significantly higher levels of semen viral load than those without warts. genital warts are caused by the human papillomavirus (hpv) and are very common among the general population. higher levels of hiv in semen may expose partners of hiv-positive men with genital warts to a greater risk of becoming infected. another session presented statistics showing t ... | 1999 | 11367261 |
| gene gun-mediated dna vaccination induces antitumor immunity against human papillomavirus type 16 e7-expressing murine tumor metastases in the liver and lungs. | dna vaccination has emerged as an attractive approach for tumor immunotherapy. the aim of this study was to evaluate the potency of dna vaccines in preventing and treating the liver and lung metastases of a human papillomavirus-16 (hpv-16) e7-expressing murine tumor (tc-1). we used the gene gun method to vaccinate c57bl/6 mice intradermally with dna vaccines containing the hpv-16 e7 gene, the e7 gene linked to the sorting signals of the lysosome-associated membrane protein-1 (sig/e7/ lamp-1), or ... | 1999 | 10637448 |
| [critical review of colpo-histological results in cervix pathology]. | the objective of this paper was to evaluate the role of squamous metaplasia in the determination of certain colposcopic appearances. | 1999 | 10638161 |
| absence of human papillomavirus sequences in early stage prostate cancer. | an etiological role for human papilloma viruses (hpvs) has been proposed for a variety of malignancies, and especially in cervical carcinomas. the role of hpvs in prostate cancer remains controversial. in particular, using primers derived from the l1 gene of hpv, it has been suggested that hpv sequences are associated with prostate malignancies. pcr and southern blot hybridization was used to detect the presence of hpv dna in fresh tissue from patients undergoing radical prostatectomy for prosta ... | 1999 | 11180776 |
| infection and cervical intraepithelial neoplasia. | the impact of sexually transmitted diseases (stds) on the development of cervical intraepithelial neoplasia (cin) has been increasingly recognized over the last 20 years. much attention has been focused on human papillomavirus (hpv) and the potential for screening for certain hpv types alongside standard cervical cytology in the hope of identifying those females at particular risk of developing high grade cin or invasive carcinoma. some infections, for example herpes simplex virus (hsv), have be ... | 1999 | 11240764 |
| papillomavirus virus-like particles as anticancer vaccines. | papillomavirus virus-like particles (vlps) are empty, non-replicative, non-infectious particles that retain conformationally correct epitopes for the generation of antibody responses to the viral capsid proteins. chimeric human papillomavirus (hpv) virus-like particles incorporating non-structural virus proteins offer an exciting approach for combined prophylactic and therapeutic vaccines against hpv-induced lesions. both hpv vlps and chimeric vlps can induce potent humoral and cellular immune r ... | 1999 | 11249689 |
| prediction of recurrent and residual cervical dysplasia by human papillomavirus detection among patients with abnormal cytology. | to determine the discriminative capacity of human papillomavirus (hpv) dna testing for recurrent and residual cervical dysplasia, 43 patients with abnormal cytology after treatment for cervical dysplasia were tested for the presence of hpv dna by pcr. an endocervical curettage was performed in all patients for histological examination. sixteen of the 43 patients showed moderate or severe dysplasia. the hpv test was positive in all 16 patients with recurrent or residual dysplasia and negative in ... | 1999 | 10021301 |
| combination therapy with podophyllin and vidarabine for human papillomavirus positive cervical intraepithelial neoplasia. | our aim was to establish an effective non-surgical treatment for cervical intraepithelial neoplasia (cin) through inactivation of human papillomavirus (hpv), the major etiological agent for this disease. we show that vidarabine, a dna polymerase inhibitor, suppressed growth and hpv gene expression in human cervical keratinocytes immortalized by hpv or in cervical cancer cell lines. expression of hpv-16 e6 and e7 proteins in normal cervical keratinocytes sensitized cells to apoptosis in the prese ... | 1999 | 10022988 |
| evaluation of human papillomavirus-consensus primers for hpv detection by the polymerase chain reaction. | cervical cancer is one of the most frequently found cancers in women and appears to have a viral aetiology. substantial evidence points to the human papillomaviruses (hpv) as the infectious agents and there is considerable interest in identifying and accurately typing the viruses. since hpvs now comprise more than 100 different hpv types, the polymerase chain reaction (pcr) has been the preferred methodology for virus identification and typing on isolated dna. in that context, five commonly empl ... | 1999 | 10024428 |
| a possible involvement of aberrant expression of the fhit gene in the carcinogenesis of squamous cell carcinoma of the uterine cervix. | to investigate involvement of an aberrant expression of the fhit (fragile histidine triad) gene in the process of carcinogenesis and progression in cervical carcinoma, we examined its expression by the reverse transcriptase polymerase chain reaction (rt-pcr) and cdna sequence method in 32 cervical invasive carcinomas (25 squamous cell carcinomas and seven adeno- or adenosquamous carcinomas) and 18 of its precursor lesions [four low-grade and 14 high-grade cervical intraepithelial neoplasias (cin ... | 1999 | 10027335 |
| cervicovaginal human papillomavirus infection in human immunodeficiency virus-1 (hiv)-positive and high-risk hiv-negative women. | human papillomavirus (hpv) infection is associated with precancerous cervical squamous intraepithelial lesions commonly seen among women infected with human immunodeficiency virus-1 (hiv). we characterized hpv infection in a large cohort of hiv-positive and hiv-negative women participating in the women's interagency hiv study to determine the prevalence of and risk factors for cervicovaginal hpv infection in hiv-positive women. | 1999 | 10037100 |
| the psychosexual impact of human papillomavirus cervical infections. | occult human papillomavirus (hpv) infection, present in approximately 20% of women in the united states, is usually sexually transmitted, associated with substantial health risks, and unpredictable in its resolution. the potential for adverse psychosexual alterations due to hpv infection in women considered at low risk for bacterial sexually transmitted diseases is substantial, but data is lacking. | 1999 | 10037541 |
| two posttranscriptional pathways that regulate p21(cip1/waf1/sdi1) are identified by hpv16-e6 interaction and correlate with life span and cellular senescence. | the p21((cip1/waf1/sdi1)) protein is a cyclin-dependent kinase inhibitor that is induced in normal human fibroblasts (nhf) following dna damage, following serum stimulation, and at cellular senescence. expression of the human papilloma virus 16 e6 oncoprotein in nhf cells results in the loss of the p21 protein, independent of mrna level under most conditions. the p21 protein levels in nhf-e6 cells remained low following dna damage or serum stimulation even though mrna levels increased. in contra ... | 1999 | 10047459 |
| the introduction of dominant-negative p53 mutants suppresses temperature shift-induced senescence in immortal human fibroblasts expressing a thermolabile sv40 large t antigen. | immortal human fibroblasts, svts8 cells, which express a heat-labile sv40 large t antigen, induces a senescence-like phenomenon in response to upward shift in temperature. cells with arrested division show strong induction of senescence-associated beta-galactosidase. we examined how p53 and prb are involved in this phenomenon since they are major targets of the t antigen. transfection of cells with plasmids encoding the wild-type t antigen or human papilloma virus type 16 e6/e7 proteins complete ... | 1999 | 10050041 |
| human papilloma virus type 16 e6 gene expression in oral exophytic epithelial lesions as detected by in situ rtpcr. | we adopted an in situ reverse transcriptase polymerase chain reaction method of detecting and determining the frequency of early (e6) gene expression of human papilloma virus type 16 at the individual cell level in a sample of oral exophytic lesions with various degrees of epithelial hyperplasia and dysplasia in immunosuppressed and immunocompetent patients. | 1999 | 10052376 |
| risk factors for abnormal anal cytology in young heterosexual women. | although anal cancers are up to four times more common in women than men, little is known about the natural history of anal human papillomavirus (hpv) infections and hpv-related anal lesions in women. this study reports on the prevalence of and risks for anal cytological abnormalities over a 1-year period in a cohort of young women participating in a study of the natural history of cervical hpv infection. in addition to their regularly scheduled sexual behavior interviews and cervical testing, c ... | 1999 | 10067816 |